MC08g1910 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1910
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionmuscle M-line assembly protein unc-89-like isoform X2
LocationMC08: 27300277 .. 27302955 (-)
RNA-Seq ExpressionMC08g1910
SyntenyMC08g1910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAGTATGAATTTGTCAATGGCCACTAACAGAACTGATGGTGGTAATCTGAGAAGATTTTCGATGGGAAAAGCAAGCTTATCGGGTATCGATGATCAAATTAGTTCTCGTAGATCCTCTACTGGTTCTTGTCATGATTTTTGCAAGTATGGACATAAACATTCATTAGAAACGAAGGCCAGAGTTCCCTTACTGAAAAGAGCAATGAAAAAGTCACTCAATGGCCAAAATTCAGACCTGGTTGTAGCCATGCCCAAGAAAGGGAAGCCGCCCCCTGTTCCTGTGACCAAGTTGAAGACTTCGCCTGATTTGGGGACATGTATTACTGGTGGCATCGACGTGGTAAAACGGGTGGTGCCTGTAAATTCTCCGGCCAGGCGAAGTCCAGTAGAGATTGTAGATATGAATGAAAGTAAGAAACATACAGTGCCAGTAAATTCTCCTACCAGGCGTAATTCAATAGGGATTGAAATTATGAATGAAAATAAGGAAAGGGTGGCACCTGTAACTTCCCCGAGTAGGCGAAGTCTGGTAGAGATTGAAGTTATGAATGAAAGTAAGGAACAAGTAGTGCCTGTAAATTCTTCTAGCAGGCAAAGTCCAGCAGAAATTGAAGTTATGAACGAAAGTAAGAAATGTGTAGTGCCAGTAAATTCTTCTACAAGGCAAAGCTCATTAGGGACTGAAGTTATGAATGAAAATAAGGAAAGAGTGGCAGCTGTAACTTCTCCGAGCAGGCGAAGTTCAGTAGGGGTTGAGGTTATGAATGAAAGTAAGGAACGAGTAGTACCCGTAAATTCTTCTAGCAGGCAAAGTCCAGTAGAGATTGAAGTTATGAACGAAGGTAAGAAGCGTGTAGTGCCAGTAAATTTTTCTACAAGGCGAAGTTCATTAGGGCCTGAAGTTATGAATGAAAATAAGGAAAGAGTGGCAGCTGCAACTTCTCCGAGTAGGCGAAATTCAGTAAAGATTGAAGCTATGAATGAAAGTAAGGGACGAGTAGTGCCTATAAATTCTTCTAGCAGGCAATGTCCAGTAGATATTGAAGTTGTGAATGAAAGTAAGAAACGTGTAGTGCCAGTAAATTCTTCTACCAGGCGAATTTCATTAGGGATTGAAGTTATGAAGGAAAATAAGGAAAGAGTGGCAGCTGTAACTTCTCCGAGTAGGCGAAGTCCGGTAAAGATTGAAGTTATGAATGAAAGTAAGGAACGAGTAGTGCCTATAAATTCTTCTAGCAGGCAATGTCCAGTAGATATTGAAGTTGTGAACGAAAGTAAGAAACGTGTAGTGCCAGTAAATTCTTCTACCAGGCGAAGTTCATTAGTGATTGAAGCTATGAATGAAAATAAGGAAAGAGTGGCAGCTCTAGCATCTTCGAGTAGGCAAAGTCCGGTAGAGATTGAAGTTATAAATGAAAGTAAGGAACAAGTAGTGCCCGTGACTTCTTCTAGCATGCAAAGTCCAGCAGAGACTGAAGTTATGAATGAAAGTAAGGAACTGGTAGTGCCCGTAAATTCTCCTAGTAGGCAAAATCCATCAAAGATTGAAGTTACGAAGGAACGTAAGAAACCGCTGGTCAAGGCAAAAACTTCGCCCAAAACCCATAAACCCAAAATTCATCTAACCACAAAGCAGGTAGTTTTCTCGCCTAGAAAATCTGCCAACTCTCCAAAACAAGCCTTGATCAATAATGGAGAAGTGAGAGTTTCCAAGAGGCTCAACAGTTTGTTGAAGCCGAAAACTTTGAAAGAAAAATCAATGATCTCTTCTGGCTCTTTTGGGAATATTGCAGTCCACAGACACGACAGTTCTGAGACAGGTGAAGGATCAAGGACCCCTAAACGAATTGGAACAAGAATGGCGGGAAATCAAGTAGTGAAGTTGGATCCAAAATCTATTGATGCTGTTGGAGATTCACCTGCGATAAAGAACAAGAACACGAGAGTTGTTTCTCGTGTTATAACTCAGAACAAAATGAGAAGAGCTCCGACCAAGGAAGTGCAGATTGTTGAACCACAGGAGAAAACCTTGTATGTCATCAATACGGAAACTAAGAACACCAATGTTATGGAATCTGACCACAATGAAAAGCATGGTTTGAATCTGCGTCCAAAGCAGCCATCATCACCATACCAGTCATCATCCTTAGCAAATTGCTCATCTCTTTCACCCCCGAAGGAAGACTGCAGTGGAACTAAATCTACAAGAAGCAAAGCAAATGCCACGTCTTCGGGACTGAACAAACAGGGTGGCATAAAAATAGGCCGGTCCCCTAGAATGCTTCAAACCAAAGGAAAGGATTCTTCATCTTTGAGCGTAAGTTTCAAGAAGGGAAAGGCAGTCGATCTTCATTCCGAAAATCCTAGTCCAACGAGGCTCAAATTTATAAGAGGAAGATCGTTCGGGGACAACGAAAAGAGCAAGGATGGACAGAGAACAAGCTTCAAGAAGGTAGTAAACAAGGGGATTTCCAAGGATTCCATACCATCATCAGAGAAAGTAGTTTTAAGACATCAAAATGTGCAGGGGAAGAAAGATACCCAGGTTTTGTTTAATAAAGTCATAGCGGAAACTGCGGGAAAACTCGTTCGAAACCAGAAGGGTAAGGTGAAGGCCTTGGTAGGAGCATTTGAGAAAGTGATCTCCCTCCAAGACAAGAAACCTTCTGTAAGAACC

mRNA sequence

GGGAGTATGAATTTGTCAATGGCCACTAACAGAACTGATGGTGGTAATCTGAGAAGATTTTCGATGGGAAAAGCAAGCTTATCGGGTATCGATGATCAAATTAGTTCTCGTAGATCCTCTACTGGTTCTTGTCATGATTTTTGCAAGTATGGACATAAACATTCATTAGAAACGAAGGCCAGAGTTCCCTTACTGAAAAGAGCAATGAAAAAGTCACTCAATGGCCAAAATTCAGACCTGGTTGTAGCCATGCCCAAGAAAGGGAAGCCGCCCCCTGTTCCTGTGACCAAGTTGAAGACTTCGCCTGATTTGGGGACATGTATTACTGGTGGCATCGACGTGGTAAAACGGGTGGTGCCTGTAAATTCTCCGGCCAGGCGAAGTCCAGTAGAGATTGTAGATATGAATGAAAGTAAGAAACATACAGTGCCAGTAAATTCTCCTACCAGGCGTAATTCAATAGGGATTGAAATTATGAATGAAAATAAGGAAAGGGTGGCACCTGTAACTTCCCCGAGTAGGCGAAGTCTGGTAGAGATTGAAGTTATGAATGAAAGTAAGGAACAAGTAGTGCCTGTAAATTCTTCTAGCAGGCAAAGTCCAGCAGAAATTGAAGTTATGAACGAAAGTAAGAAATGTGTAGTGCCAGTAAATTCTTCTACAAGGCAAAGCTCATTAGGGACTGAAGTTATGAATGAAAATAAGGAAAGAGTGGCAGCTGTAACTTCTCCGAGCAGGCGAAGTTCAGTAGGGGTTGAGGTTATGAATGAAAGTAAGGAACGAGTAGTACCCGTAAATTCTTCTAGCAGGCAAAGTCCAGTAGAGATTGAAGTTATGAACGAAGGTAAGAAGCGTGTAGTGCCAGTAAATTTTTCTACAAGGCGAAGTTCATTAGGGCCTGAAGTTATGAATGAAAATAAGGAAAGAGTGGCAGCTGCAACTTCTCCGAGTAGGCGAAATTCAGTAAAGATTGAAGCTATGAATGAAAGTAAGGGACGAGTAGTGCCTATAAATTCTTCTAGCAGGCAATGTCCAGTAGATATTGAAGTTGTGAATGAAAGTAAGAAACGTGTAGTGCCAGTAAATTCTTCTACCAGGCGAATTTCATTAGGGATTGAAGTTATGAAGGAAAATAAGGAAAGAGTGGCAGCTGTAACTTCTCCGAGTAGGCGAAGTCCGGTAAAGATTGAAGTTATGAATGAAAGTGAAGGATCAAGGACCCCTAAACGAATTGGAACAAGAATGGCGGGAAATCAAGTAGTGAAGTTGGATCCAAAATCTATTGATGCTGTTGGAGATTCACCTGCGATAAAGAACAAGAACACGAGAGTTGTTTCTCGTGTTATAACTCAGAACAAAATGAGAAGAGCTCCGACCAAGGAAGTGCAGATTGTTGAACCACAGGAGAAAACCTTGTATGTCATCAATACGGAAACTAAGAACACCAATGTTATGGAATCTGACCACAATGAAAAGCATGGTTTGAATCTGCGTCCAAAGCAGCCATCATCACCATACCAGTCATCATCCTTAGCAAATTGCTCATCTCTTTCACCCCCGAAGGAAGACTGCAGTGGAACTAAATCTACAAGAAGCAAAGCAAATGCCACGTCTTCGGGACTGAACAAACAGGGTGGCATAAAAATAGGCCGGTCCCCTAGAATGCTTCAAACCAAAGGAAAGGATTCTTCATCTTTGAGCGTAAGTTTCAAGAAGGGAAAGGCAGTCGATCTTCATTCCGAAAATCCTAGTCCAACGAGGCTCAAATTTATAAGAGGAAGATCGTTCGGGGACAACGAAAAGAGCAAGGATGGACAGAGAACAAGCTTCAAGAAGGTAGTAAACAAGGGGATTTCCAAGGATTCCATACCATCATCAGAGAAAGTAGTTTTAAGACATCAAAATGTGCAGGGGAAGAAAGATACCCAGGTTTTGTTTAATAAAGTCATAGCGGAAACTGCGGGAAAACTCGTTCGAAACCAGAAGGGTAAGGTGAAGGCCTTGGTAGGAGCATTTGAGAAAGTGATCTCCCTCCAAGACAAGAAACCTTCTGTAAGAACC

Coding sequence (CDS)

GGGAGTATGAATTTGTCAATGGCCACTAACAGAACTGATGGTGGTAATCTGAGAAGATTTTCGATGGGAAAAGCAAGCTTATCGGGTATCGATGATCAAATTAGTTCTCGTAGATCCTCTACTGGTTCTTGTCATGATTTTTGCAAGTATGGACATAAACATTCATTAGAAACGAAGGCCAGAGTTCCCTTACTGAAAAGAGCAATGAAAAAGTCACTCAATGGCCAAAATTCAGACCTGGTTGTAGCCATGCCCAAGAAAGGGAAGCCGCCCCCTGTTCCTGTGACCAAGTTGAAGACTTCGCCTGATTTGGGGACATGTATTACTGGTGGCATCGACGTGGTAAAACGGGTGGTGCCTGTAAATTCTCCGGCCAGGCGAAGTCCAGTAGAGATTGTAGATATGAATGAAAGTAAGAAACATACAGTGCCAGTAAATTCTCCTACCAGGCGTAATTCAATAGGGATTGAAATTATGAATGAAAATAAGGAAAGGGTGGCACCTGTAACTTCCCCGAGTAGGCGAAGTCTGGTAGAGATTGAAGTTATGAATGAAAGTAAGGAACAAGTAGTGCCTGTAAATTCTTCTAGCAGGCAAAGTCCAGCAGAAATTGAAGTTATGAACGAAAGTAAGAAATGTGTAGTGCCAGTAAATTCTTCTACAAGGCAAAGCTCATTAGGGACTGAAGTTATGAATGAAAATAAGGAAAGAGTGGCAGCTGTAACTTCTCCGAGCAGGCGAAGTTCAGTAGGGGTTGAGGTTATGAATGAAAGTAAGGAACGAGTAGTACCCGTAAATTCTTCTAGCAGGCAAAGTCCAGTAGAGATTGAAGTTATGAACGAAGGTAAGAAGCGTGTAGTGCCAGTAAATTTTTCTACAAGGCGAAGTTCATTAGGGCCTGAAGTTATGAATGAAAATAAGGAAAGAGTGGCAGCTGCAACTTCTCCGAGTAGGCGAAATTCAGTAAAGATTGAAGCTATGAATGAAAGTAAGGGACGAGTAGTGCCTATAAATTCTTCTAGCAGGCAATGTCCAGTAGATATTGAAGTTGTGAATGAAAGTAAGAAACGTGTAGTGCCAGTAAATTCTTCTACCAGGCGAATTTCATTAGGGATTGAAGTTATGAAGGAAAATAAGGAAAGAGTGGCAGCTGTAACTTCTCCGAGTAGGCGAAGTCCGGTAAAGATTGAAGTTATGAATGAAAGTGAAGGATCAAGGACCCCTAAACGAATTGGAACAAGAATGGCGGGAAATCAAGTAGTGAAGTTGGATCCAAAATCTATTGATGCTGTTGGAGATTCACCTGCGATAAAGAACAAGAACACGAGAGTTGTTTCTCGTGTTATAACTCAGAACAAAATGAGAAGAGCTCCGACCAAGGAAGTGCAGATTGTTGAACCACAGGAGAAAACCTTGTATGTCATCAATACGGAAACTAAGAACACCAATGTTATGGAATCTGACCACAATGAAAAGCATGGTTTGAATCTGCGTCCAAAGCAGCCATCATCACCATACCAGTCATCATCCTTAGCAAATTGCTCATCTCTTTCACCCCCGAAGGAAGACTGCAGTGGAACTAAATCTACAAGAAGCAAAGCAAATGCCACGTCTTCGGGACTGAACAAACAGGGTGGCATAAAAATAGGCCGGTCCCCTAGAATGCTTCAAACCAAAGGAAAGGATTCTTCATCTTTGAGCGTAAGTTTCAAGAAGGGAAAGGCAGTCGATCTTCATTCCGAAAATCCTAGTCCAACGAGGCTCAAATTTATAAGAGGAAGATCGTTCGGGGACAACGAAAAGAGCAAGGATGGACAGAGAACAAGCTTCAAGAAGGTAGTAAACAAGGGGATTTCCAAGGATTCCATACCATCATCAGAGAAAGTAGTTTTAAGACATCAAAATGTGCAGGGGAAGAAAGATACCCAGGTTTTGTTTAATAAAGTCATAGCGGAAACTGCGGGAAAACTCGTTCGAAACCAGAAGGGTAAGGTGAAGGCCTTGGTAGGAGCATTTGAGAAAGTGATCTCCCTCCAAGACAAGAAACCTTCTGTAAGAACC

Protein sequence

GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKARVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVPVNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEIEVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVPVNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNESEGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKMRRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANCSSLSPPKEDCSGTKSTRSKANATSSGLNKQGGIKIGRSPRMLQTKGKDSSSLSVSFKKGKAVDLHSENPSPTRLKFIRGRSFGDNEKSKDGQRTSFKKVVNKGISKDSIPSSEKVVLRHQNVQGKKDTQVLFNKVIAETAGKLVRNQKGKVKALVGAFEKVISLQDKKPSVRT
Homology
BLAST of MC08g1910 vs. NCBI nr
Match: XP_022144538.1 (flocculation protein FLO11-like isoform X1 [Momordica charantia] >XP_022144539.1 flocculation protein FLO11-like isoform X1 [Momordica charantia])

HSP 1 Score: 1206 bits (3119), Expect = 0.0
Identity = 687/893 (76.93%), Postives = 687/893 (76.93%), Query Frame = 0

Query: 1   GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA 60
           GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA
Sbjct: 4   GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA 63

Query: 61  RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP 120
           RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP
Sbjct: 64  RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP 123

Query: 121 VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI 180
           VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI
Sbjct: 124 VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI 183

Query: 181 EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA 240
           EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA
Sbjct: 184 EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA 243

Query: 241 VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP 300
           VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP
Sbjct: 244 VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP 303

Query: 301 EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP 360
           EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP
Sbjct: 304 EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP 363

Query: 361 VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNES------------------ 420
           VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNES                  
Sbjct: 364 VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNESKERVVPINSSSRQCPVDI 423

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 424 EVVNESKKRVVPVNSSTRRSSLVIEAMNENKERVAALASSSRQSPVEIEVINESKEQVVP 483

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 484 VTSSSMQSPAETEVMNESKELVVPVNSPSRQNPSKIEVTKERKKPLVKAKTSPKTHKPKI 543

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 544 HLTTKQVVFSPRKSANSPKQALINNGEVRVSKRLNSLLKPKTLKEKSMISSGSFGNIAVH 603

Query: 601 --------EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM 660
                   EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM
Sbjct: 604 RHDSSETGEGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM 663

Query: 661 RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC 687
           RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC
Sbjct: 664 RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC 723

BLAST of MC08g1910 vs. NCBI nr
Match: XP_022144540.1 (flocculation protein FLO11-like isoform X2 [Momordica charantia])

HSP 1 Score: 1200 bits (3105), Expect = 0.0
Identity = 684/890 (76.85%), Postives = 684/890 (76.85%), Query Frame = 0

Query: 1   GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA 60
           GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA
Sbjct: 4   GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA 63

Query: 61  RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP 120
           RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP
Sbjct: 64  RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP 123

Query: 121 VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI 180
           VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI
Sbjct: 124 VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI 183

Query: 181 EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA 240
           EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA
Sbjct: 184 EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA 243

Query: 241 VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP 300
           VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP
Sbjct: 244 VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP 303

Query: 301 EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP 360
           EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP
Sbjct: 304 EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP 363

Query: 361 VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNES------------------ 420
           VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNES                  
Sbjct: 364 VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNESKERVVPINSSSRQCPVDI 423

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 424 EVVNESKKRVVPVNSSTRRSSLVIEAMNENKERVAALASSSRQSPVEIEVINESKEQVVP 483

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 484 VTSSSMQSPAETEVMNESKELVVPVNSPSRQNPSKIEVTKERKKPLVKAKTSPKTHKPKI 543

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 544 HLTTKQVVFSPRKSANSPKQALINNGEVRVSKRLNSLLKPKTLKEKSMISSGSFGNIAVH 603

Query: 601 --------EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM 660
                   EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM
Sbjct: 604 RHDSSETGEGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM 663

Query: 661 RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC 684
           RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC
Sbjct: 664 RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC 723

BLAST of MC08g1910 vs. NCBI nr
Match: KAG6607113.1 (hypothetical protein SDJN03_00455, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 580 bits (1494), Expect = 3.90e-195
Identity = 383/768 (49.87%), Postives = 483/768 (62.89%), Query Frame = 0

Query: 1   GSMNLSMA--TNRTDGGNLRRFSMGKASLSGIDDQ--ISSRRSSTGSCHDFCKYGHKHSL 60
           GSM+LSM+  T+R+DG  LRR SMGKA    ID+Q     RRSS GSCHD CKYGH HSL
Sbjct: 4   GSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSL 63

Query: 61  ETKARVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVK 120
           ETKARVPLLKRAMKK+L+ QNSD  V +P+K     V VTK K SP  GTCI+GG DV+K
Sbjct: 64  ETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHS--VRVTKSKVSPSSGTCISGGTDVIK 123

Query: 121 RVVPVNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGI-------------------- 180
           RVVP++SP+RR PVE    ++SK+    V +P R++   +                    
Sbjct: 124 RVVPISSPSRR-PVETGVASKSKEQATLVKAPNRQSETEVTSKSKEQATLVKAPNGQSET 183

Query: 181 EIMNENKERVAPVTSPSRRSLV-----------EIEVMNESKEQVVPVNSSSRQSPAEIE 240
           E+ +E+KE   PV SPS++  V            IEV++ESKE +VPVNS +RQ    IE
Sbjct: 184 EVTSESKEPEVPVGSPSKKIEVIMVLPVNSPTKRIEVISESKELIVPVNSPTRQ----IE 243

Query: 241 VMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVTSPSRRSSVGVEVMNESKERVVPV 300
           V++ESK+  VP+NS TRQ     EV++E+KE V  + SP+R+    +EV++ES+E VVPV
Sbjct: 244 VISESKELAVPLNSPTRQ----IEVISESKELVVPLNSPTRQ----IEVISESQELVVPV 303

Query: 301 NSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEVMNENKERVAAATSPSRRNSVKIE 360
           NS +RQSPVE+EV +E  +RVVP N ++R SS+  E  ++N ERV   TSPS R+ V+I 
Sbjct: 304 NSPTRQSPVEMEVSSESSERVVPENSTSRHSSVEIEATSKNIERVVPETSPSGRSRVEIG 363

Query: 361 AMNESKGRVVPINSS------SRQCPVDIEVVNESKKRVVP------------------- 420
             +E K  VV   SS      +R+ P+  E +NE K++V                     
Sbjct: 364 VRSEIKEPVVVAESSLSRQGSNRRSPLKSEAMNEGKEQVAKTKTKPITSKPKFHKTTKQV 423

Query: 421 VNSSTRRISL------------GIEVMKENKERVAAVTSPSRRSPVKIEVMNES---EGS 480
           V SST+  S             G  + K    +  +VTS      +     N S   EG+
Sbjct: 424 VYSSTKSESSPKQALASVAEAGGDSLSKPKALKAKSVTSSGPSGNIAAHSNNNSKSCEGA 483

Query: 481 RTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKMRRAPTKEVQIV 540
            T K  G ++    ++  DP S+D V + PAIKNKN++VVS+V +QNK RRA  KE   V
Sbjct: 484 GTLKGNGIKVVEKSIITTDPNSVDTVVNLPAIKNKNSKVVSQVKSQNKTRRAQAKEASSV 543

Query: 541 EPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANCSSLSPPKEDCS 600
           E QEK L+VIN ETK T ++ESD N+KHGL    K P+S      LAN  SLSP +ED  
Sbjct: 544 ESQEKILHVINVETKKTKLVESDQNDKHGLKGYQKSPTS------LANGPSLSPLREDSG 603

Query: 601 GTKSTRSKANATSSGLNKQGGIKI---------GRSPRMLQTKGKDSSSLSVSFKKGKAV 660
           GTK T+ +ANAT SG  K GGIK          G+SPRMLQTKGKDSSS S+SF+  K +
Sbjct: 604 GTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPRMLQTKGKDSSSFSLSFRNKKVI 663

Query: 661 DLHSENPSPTRLKFIRGRSFGDNEKSKDGQRTSFKKVVNKGISKDSIPSSEKVVLRHQNV 684
           DL S+   PTRLKF+ G+S GDN+KSKDG+RTS KK + KGISK+S P SEKVVLRHQ+V
Sbjct: 664 DLDSKRHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGIVKGISKNSTPPSEKVVLRHQDV 723

BLAST of MC08g1910 vs. NCBI nr
Match: XP_022949115.1 (muscle M-line assembly protein unc-89-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 577 bits (1488), Expect = 5.89e-194
Identity = 387/791 (48.93%), Postives = 486/791 (61.44%), Query Frame = 0

Query: 1   GSMNLSMA--TNRTDGGNLRRFSMGKASLSGIDDQ--ISSRRSSTGSCHDFCKYGHKHSL 60
           GSM+LSM+  T+R+DG  LRR SMGKA    ID+Q     RRSS GSCHD CKYGH HSL
Sbjct: 4   GSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSL 63

Query: 61  ETKARVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVK 120
           ETKARVPLLKRAMKK+L+ QNSD  V +P+K     V VTK K SP  GTCI+GG DV+K
Sbjct: 64  ETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHS--VRVTKSKVSPSSGTCISGGTDVIK 123

Query: 121 RVVPVNSPARR-----------------------SPVEIVDMN----------------- 180
           RVVP++SP+RR                       S  E+   +                 
Sbjct: 124 RVVPISSPSRRPVETGVTSKSKEQATLVKAPNRQSETEVTSKSKEQATLVKAPNGQSETE 183

Query: 181 ---ESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLV-----------EIEV 240
              ESK+  VPV SPT++    IE+++ENK  V P+ SPS++  V            IEV
Sbjct: 184 VTSESKEPEVPVGSPTKQ----IEVISENKALVVPMNSPSKKIEVIMVLPVNSPTKRIEV 243

Query: 241 MNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVT 300
           ++ESKE +VPVNS +RQ    IEV++ESK+  VP+NS TRQ     EV++E+KE V  + 
Sbjct: 244 ISESKELIVPVNSPTRQ----IEVISESKELAVPLNSPTRQ----IEVISESKELVVPLN 303

Query: 301 SPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEV 360
           SP+R+    +EV++ES+E VVPVNS +RQSPVE+EV +E  +RVVP N ++R SS+  E 
Sbjct: 304 SPTRQ----IEVISESQELVVPVNSPTRQSPVEMEVSSESSERVVPENSTSRHSSVEIEA 363

Query: 361 MNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSS------SRQCPVDIEVVNESKK 420
            ++N ERV   TSPS R+ V+I   +E K  VV   SS      +R+ P+  E +NE K+
Sbjct: 364 TSKNMERVVPETSPSGRSRVEIGVRSEIKEPVVVAESSLSRQGSNRRSPLKSEAMNEGKE 423

Query: 421 RVVP-------------------VNSSTRRISL------------GIEVMKENKERVAAV 480
           +V                     V SST+  S             G  + K    +  +V
Sbjct: 424 QVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALASVAEAGGDSLSKPKALKAKSV 483

Query: 481 TSPSRRSPVKIEVMNES---EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNT 540
           TS      +     N S   EG+ T K  GT++    ++  DP S+D V + PAIKNKN+
Sbjct: 484 TSSGPSGNIAAHSNNNSKSCEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAIKNKNS 543

Query: 541 RVVSRVITQNKMRRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQP 600
           +VVS+V +QNK RRA  KE   VE QEK L+VIN ETK T ++ESD N+KHGL    K P
Sbjct: 544 KVVSQVKSQNKTRRAQAKEASSVESQEKILHVINVETKKTKLVESDQNDKHGLKGYQKSP 603

Query: 601 SSPYQSSSLANCSSLSPPKEDCSGTKSTRSKANATSSGLNKQGGIKI---------GRSP 660
           +S      LAN  SLSP +ED  GTK T+ +ANAT SG  K GGIK          G+SP
Sbjct: 604 TS------LANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSP 663

Query: 661 RMLQTKGKDSSSLSVSFKKGKAVDLHSENPSPTRLKFIRGRSFGDNEKSKDGQRTSFKKV 684
           RMLQTKGKDSSS S+SF+  K +DL S+   PTRLKF+  +S GDN+KSKDG+RTS KK 
Sbjct: 664 RMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEVKSLGDNQKSKDGRRTSLKKG 723

BLAST of MC08g1910 vs. NCBI nr
Match: XP_023523723.1 (uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo] >XP_023523724.1 uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 577 bits (1487), Expect = 9.75e-194
Identity = 387/800 (48.38%), Postives = 491/800 (61.38%), Query Frame = 0

Query: 1   GSMNLSMA--TNRTDGGNLRRFSMGKASLSGIDDQIS--SRRSSTGSCHDFCKYGHKHSL 60
           GSM+LSM+  T+R+DG  LRR SMGKA    ID+Q S   RRSS GSCHD CKYGH HSL
Sbjct: 4   GSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNSFRDRRSSIGSCHDICKYGHNHSL 63

Query: 61  ETKARVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVK 120
           ETKARVPLLKRAMKK+L+ QNSD  V +P+K     V VTK K SP  GTCI+GG DV+K
Sbjct: 64  ETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHS--VRVTKSKVSPSSGTCISGGTDVIK 123

Query: 121 RVVPVNSPARR------------------------------------------------- 180
           RVVP++SP+RR                                                 
Sbjct: 124 RVVPISSPSRRPVETGVKSKSKEQATLVKAPNRQSETEVTSKSKEQATVVKAPNGQSETE 183

Query: 181 -------------SPVEIVDM-NESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPS 240
                        SP + +++ +E+K   VP+NSPT++    IE+++E+ E V PV SP+
Sbjct: 184 VTSESKEPEVPMGSPTKQIEVISENKALVVPMNSPTKK----IEVISESNELVLPVNSPT 243

Query: 241 RRSLVEIEVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNE 300
           +R    IEV++ESKE +VPVNS ++Q    IEV++ESK+ VVP++S TRQ     EV++E
Sbjct: 244 KR----IEVISESKELIVPVNSPTKQ----IEVISESKELVVPLHSPTRQ----IEVISE 303

Query: 301 NKERVAAVTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFST 360
           +KE V  + SP+RR    +EV+ ESKE VVPVNS +RQSPVE+EV +E  +RVVP N ++
Sbjct: 304 SKELVVPLNSPTRR----IEVICESKELVVPVNSPTRQSPVEMEVSSESSERVVPENSTS 363

Query: 361 RRSSLGPEVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSS------SRQCPVD 420
           R SS+  E  ++N E+V   T PSRR+ V+I    ESK  VV   SS      +R+ P+ 
Sbjct: 364 RHSSVEIEATSKNMEQVTPETFPSRRSRVEIGVRRESKEPVVVAESSLSRQGSNRRSPLK 423

Query: 421 IEVVNESKKRVVP-------------------VNSSTRRISL------------GIEVMK 480
            E +NE K+ V                     V SST+  S             G  + K
Sbjct: 424 SEAMNEGKELVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSK 483

Query: 481 ENKERVAAVTSPSRRSPVKIEVMNES---EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGD 540
               +  +VTS      +     N S   EG+ T K  GT++    ++ +DP S+D V +
Sbjct: 484 PKALKAKSVTSSGPSGNIAAHSNNISKSGEGAGTLKGNGTKVVEKSIITMDPNSVDTVVN 543

Query: 541 SPAIKNKNTRVVSRVITQNKMRRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKH 600
            PAIKNKN++VVS+V +QNK RRA  KE   VE QEK L+VIN ETK + ++ESD N+KH
Sbjct: 544 LPAIKNKNSKVVSQVKSQNKTRRAQAKEASSVESQEKILHVINVETKKSKLLESDQNDKH 603

Query: 601 GLNLRPKQPSSPYQSSSLANCSSLSPPKEDCSGTKSTRSKANATSSGLNKQGGIKI---- 660
           GL    K PSS      LAN  SLSP +ED  GTK T+ +ANAT SG  K GGIK     
Sbjct: 604 GLKGYQKSPSS------LANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDP 663

Query: 661 -----GRSPRMLQTKGKDSSSLSVSFKKGKAVDLHSENPSPTRLKFIRGRSFGDNEKSKD 684
                G+SPRMLQTKGKDSSS S+SF+  K +DL S++  PTRLKF+ G+S GDN+KSKD
Sbjct: 664 NGVKKGKSPRMLQTKGKDSSSFSLSFRNKKVIDLDSKSHGPTRLKFMEGKSLGDNQKSKD 723

BLAST of MC08g1910 vs. ExPASy TrEMBL
Match: A0A6J1CSL1 (flocculation protein FLO11-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014195 PE=4 SV=1)

HSP 1 Score: 1206 bits (3119), Expect = 0.0
Identity = 687/893 (76.93%), Postives = 687/893 (76.93%), Query Frame = 0

Query: 1   GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA 60
           GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA
Sbjct: 4   GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA 63

Query: 61  RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP 120
           RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP
Sbjct: 64  RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP 123

Query: 121 VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI 180
           VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI
Sbjct: 124 VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI 183

Query: 181 EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA 240
           EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA
Sbjct: 184 EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA 243

Query: 241 VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP 300
           VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP
Sbjct: 244 VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP 303

Query: 301 EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP 360
           EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP
Sbjct: 304 EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP 363

Query: 361 VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNES------------------ 420
           VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNES                  
Sbjct: 364 VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNESKERVVPINSSSRQCPVDI 423

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 424 EVVNESKKRVVPVNSSTRRSSLVIEAMNENKERVAALASSSRQSPVEIEVINESKEQVVP 483

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 484 VTSSSMQSPAETEVMNESKELVVPVNSPSRQNPSKIEVTKERKKPLVKAKTSPKTHKPKI 543

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 544 HLTTKQVVFSPRKSANSPKQALINNGEVRVSKRLNSLLKPKTLKEKSMISSGSFGNIAVH 603

Query: 601 --------EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM 660
                   EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM
Sbjct: 604 RHDSSETGEGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM 663

Query: 661 RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC 687
           RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC
Sbjct: 664 RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC 723

BLAST of MC08g1910 vs. ExPASy TrEMBL
Match: A0A6J1CRX0 (flocculation protein FLO11-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014195 PE=4 SV=1)

HSP 1 Score: 1200 bits (3105), Expect = 0.0
Identity = 684/890 (76.85%), Postives = 684/890 (76.85%), Query Frame = 0

Query: 1   GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA 60
           GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA
Sbjct: 4   GSMNLSMATNRTDGGNLRRFSMGKASLSGIDDQISSRRSSTGSCHDFCKYGHKHSLETKA 63

Query: 61  RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP 120
           RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP
Sbjct: 64  RVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVVP 123

Query: 121 VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI 180
           VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI
Sbjct: 124 VNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEI 183

Query: 181 EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA 240
           EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA
Sbjct: 184 EVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAA 243

Query: 241 VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP 300
           VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP
Sbjct: 244 VTSPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGP 303

Query: 301 EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP 360
           EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP
Sbjct: 304 EVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVP 363

Query: 361 VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNES------------------ 420
           VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNES                  
Sbjct: 364 VNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNESKERVVPINSSSRQCPVDI 423

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 424 EVVNESKKRVVPVNSSTRRSSLVIEAMNENKERVAALASSSRQSPVEIEVINESKEQVVP 483

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 484 VTSSSMQSPAETEVMNESKELVVPVNSPSRQNPSKIEVTKERKKPLVKAKTSPKTHKPKI 543

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 544 HLTTKQVVFSPRKSANSPKQALINNGEVRVSKRLNSLLKPKTLKEKSMISSGSFGNIAVH 603

Query: 601 --------EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM 660
                   EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM
Sbjct: 604 RHDSSETGEGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKM 663

Query: 661 RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC 684
           RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC
Sbjct: 664 RRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANC 723

BLAST of MC08g1910 vs. ExPASy TrEMBL
Match: A0A6J1GBV9 (muscle M-line assembly protein unc-89-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452563 PE=4 SV=1)

HSP 1 Score: 577 bits (1488), Expect = 2.85e-194
Identity = 387/791 (48.93%), Postives = 486/791 (61.44%), Query Frame = 0

Query: 1   GSMNLSMA--TNRTDGGNLRRFSMGKASLSGIDDQ--ISSRRSSTGSCHDFCKYGHKHSL 60
           GSM+LSM+  T+R+DG  LRR SMGKA    ID+Q     RRSS GSCHD CKYGH HSL
Sbjct: 4   GSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSL 63

Query: 61  ETKARVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVK 120
           ETKARVPLLKRAMKK+L+ QNSD  V +P+K     V VTK K SP  GTCI+GG DV+K
Sbjct: 64  ETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHS--VRVTKSKVSPSSGTCISGGTDVIK 123

Query: 121 RVVPVNSPARR-----------------------SPVEIVDMN----------------- 180
           RVVP++SP+RR                       S  E+   +                 
Sbjct: 124 RVVPISSPSRRPVETGVTSKSKEQATLVKAPNRQSETEVTSKSKEQATLVKAPNGQSETE 183

Query: 181 ---ESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLV-----------EIEV 240
              ESK+  VPV SPT++    IE+++ENK  V P+ SPS++  V            IEV
Sbjct: 184 VTSESKEPEVPVGSPTKQ----IEVISENKALVVPMNSPSKKIEVIMVLPVNSPTKRIEV 243

Query: 241 MNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVT 300
           ++ESKE +VPVNS +RQ    IEV++ESK+  VP+NS TRQ     EV++E+KE V  + 
Sbjct: 244 ISESKELIVPVNSPTRQ----IEVISESKELAVPLNSPTRQ----IEVISESKELVVPLN 303

Query: 301 SPSRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEV 360
           SP+R+    +EV++ES+E VVPVNS +RQSPVE+EV +E  +RVVP N ++R SS+  E 
Sbjct: 304 SPTRQ----IEVISESQELVVPVNSPTRQSPVEMEVSSESSERVVPENSTSRHSSVEIEA 363

Query: 361 MNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSS------SRQCPVDIEVVNESKK 420
            ++N ERV   TSPS R+ V+I   +E K  VV   SS      +R+ P+  E +NE K+
Sbjct: 364 TSKNMERVVPETSPSGRSRVEIGVRSEIKEPVVVAESSLSRQGSNRRSPLKSEAMNEGKE 423

Query: 421 RVVP-------------------VNSSTRRISL------------GIEVMKENKERVAAV 480
           +V                     V SST+  S             G  + K    +  +V
Sbjct: 424 QVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALASVAEAGGDSLSKPKALKAKSV 483

Query: 481 TSPSRRSPVKIEVMNES---EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNT 540
           TS      +     N S   EG+ T K  GT++    ++  DP S+D V + PAIKNKN+
Sbjct: 484 TSSGPSGNIAAHSNNNSKSCEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAIKNKNS 543

Query: 541 RVVSRVITQNKMRRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQP 600
           +VVS+V +QNK RRA  KE   VE QEK L+VIN ETK T ++ESD N+KHGL    K P
Sbjct: 544 KVVSQVKSQNKTRRAQAKEASSVESQEKILHVINVETKKTKLVESDQNDKHGLKGYQKSP 603

Query: 601 SSPYQSSSLANCSSLSPPKEDCSGTKSTRSKANATSSGLNKQGGIKI---------GRSP 660
           +S      LAN  SLSP +ED  GTK T+ +ANAT SG  K GGIK          G+SP
Sbjct: 604 TS------LANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSP 663

Query: 661 RMLQTKGKDSSSLSVSFKKGKAVDLHSENPSPTRLKFIRGRSFGDNEKSKDGQRTSFKKV 684
           RMLQTKGKDSSS S+SF+  K +DL S+   PTRLKF+  +S GDN+KSKDG+RTS KK 
Sbjct: 664 RMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEVKSLGDNQKSKDGRRTSLKKG 723

BLAST of MC08g1910 vs. ExPASy TrEMBL
Match: A0A6J1GB51 (muscle M-line assembly protein unc-89-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452563 PE=4 SV=1)

HSP 1 Score: 571 bits (1471), Expect = 2.05e-191
Identity = 387/820 (47.20%), Postives = 489/820 (59.63%), Query Frame = 0

Query: 1   GSMNLSMA--TNRTDGGNLRRFSMGKASLSGIDDQ--ISSRRSSTGSCHDFCKYGHKHSL 60
           GSM+LSM+  T+R+DG  LRR SMGKA    ID+Q     RRSS GSCHD CKYGH HSL
Sbjct: 4   GSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSL 63

Query: 61  ETKARVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVK 120
           ETKARVPLLKRAMKK+L+ QNSD  V +P+K     V VTK K SP  GTCI+GG DV+K
Sbjct: 64  ETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHS--VRVTKSKVSPSSGTCISGGTDVIK 123

Query: 121 RVVPVNSPARRSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRS 180
           RVVP++SP+RR PVE    ++SK+    V +P R++    E+ +++KE+   V +P+R+S
Sbjct: 124 RVVPISSPSRR-PVETGVTSKSKEQATLVKAPNRQSET--EVTSKSKEQATLVKAPNRQS 183

Query: 181 LVEIEVMNESKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKE 240
             E EV ++SKEQ   V + + QS  E EV +ESK+  VPV S T+Q     EV++ENK 
Sbjct: 184 --ETEVTSKSKEQATLVKAPNGQS--ETEVTSESKEPEVPVGSPTKQ----IEVISENKA 243

Query: 241 RVAAVTSPSRRSSV-----------GVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKR 300
            V  + SPS++  V            +EV++ESKE +VPVNS +RQ    IEV++E K+ 
Sbjct: 244 LVVPMNSPSKKIEVIMVLPVNSPTKRIEVISESKELIVPVNSPTRQ----IEVISESKEL 303

Query: 301 VVPVNFSTRRSSLGPEVMNENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCP 360
            VP+N  TR+     EV++E+KE V    SP+R+    IE ++ES+  VVP+NS +RQ P
Sbjct: 304 AVPLNSPTRQI----EVISESKELVVPLNSPTRQ----IEVISESQELVVPVNSPTRQSP 363

Query: 361 VDIEVVNESKKRVVPVNSSTRRISLGIEVMKENKERVAAVTSPS---------------- 420
           V++EV +ES +RVVP NS++R  S+ IE   +N ERV   TSPS                
Sbjct: 364 VEMEVSSESSERVVPENSTSRHSSVEIEATSKNMERVVPETSPSGRSRVEIGVRSEIKEP 423

Query: 421 --------------RRSPVKIEVMNES--------------------------------- 480
                         RRSP+K E MNE                                  
Sbjct: 424 VVVAESSLSRQGSNRRSPLKSEAMNEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSE 483

Query: 481 -------------------------------------------------EGSRTPKRIGT 540
                                                            EG+ T K  GT
Sbjct: 484 SSPKQALASVAEAGGDSLSKPKALKAKSVTSSGPSGNIAAHSNNNSKSCEGAGTLKGNGT 543

Query: 541 RMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKMRRAPTKEVQIVEPQEKTLY 600
           ++    ++  DP S+D V + PAIKNKN++VVS+V +QNK RRA  KE   VE QEK L+
Sbjct: 544 KVVEKSIITTDPNSVDTVVNLPAIKNKNSKVVSQVKSQNKTRRAQAKEASSVESQEKILH 603

Query: 601 VINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANCSSLSPPKEDCSGTKSTRSK 660
           VIN ETK T ++ESD N+KHGL    K P+S      LAN  SLSP +ED  GTK T+ +
Sbjct: 604 VINVETKKTKLVESDQNDKHGLKGYQKSPTS------LANGPSLSPLREDSGGTKYTKYE 663

Query: 661 ANATSSGLNKQGGIKI---------GRSPRMLQTKGKDSSSLSVSFKKGKAVDLHSENPS 684
           ANAT SG  K GGIK          G+SPRMLQTKGKDSSS S+SF+  K +DL S+   
Sbjct: 664 ANATVSGSKKHGGIKKEDDPNGVKKGKSPRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHG 723

BLAST of MC08g1910 vs. ExPASy TrEMBL
Match: A0A6J1K8S1 (muscle M-line assembly protein unc-89-like isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111492710 PE=4 SV=1)

HSP 1 Score: 567 bits (1462), Expect = 1.32e-190
Identity = 380/778 (48.84%), Postives = 486/778 (62.47%), Query Frame = 0

Query: 1   GSMNLSMA--TNRTDGGNLRRFSMGKASLSGIDDQIS--SRRSSTGSCHDFCKYGHKHSL 60
           GSM+LSM+  T+R+DG  LRR SMGKA    ID+Q S   RRSS GSCHD CKYGH HSL
Sbjct: 4   GSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNSFRDRRSSIGSCHDICKYGHNHSL 63

Query: 61  ETKARVPLLKRAMKKSLNGQNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVK 120
           ETKARVPLLKRAMKK+L+ QNSD  V +P+K     V VTK K SP   TCI+GG DV+K
Sbjct: 64  ETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHS--VCVTKSKVSPSSKTCISGGTDVIK 123

Query: 121 RVVPVNSPARR----------------------------------------SPVEIVDM- 180
           RVVP++SP+RR                                        SP + +++ 
Sbjct: 124 RVVPISSPSRRLVETGVMSKSKEQATLVKAPNGQSETEVTSESKEPEAPVGSPTKQIEVI 183

Query: 181 NESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRRSLVEIEVMNESKEQVVPVNS 240
           +E+K+  VP+NSPT++    IE+++E+ E V PV SP++R    IEV++ESKE +VPVNS
Sbjct: 184 SENKELVVPMNSPTKK----IEVISESNEVVLPVNSPTKR----IEVISESKELIVPVNS 243

Query: 241 SSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKERVAAVTSPSRRSSVGVEVM 300
            +RQ    IEV++ESK+ V+P+NS TRQ     EV++ +KE V  + SP+R+    +EV+
Sbjct: 244 PTRQ----IEVISESKELVMPLNSPTRQ----IEVISGSKELVVPLNSPTRQ----IEVI 303

Query: 301 NESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEVMNENKERVAAATS 360
           +ESKE VVPVNS +RQSPVE+EV +E  +RVVP N ++R SS+  E  ++N ERV   T 
Sbjct: 304 SESKELVVPVNSPTRQSPVEMEVSSESSERVVPENSTSRHSSVEIEATSKNMERVVPETF 363

Query: 361 PSRRNSVKIEAMNESKGRVVPINSS------SRQCPVDIEVVNESKKRVVP--------- 420
           PSRR+ V+I   +ESK  VV   SS      +R+ P+  E +NE K+ V           
Sbjct: 364 PSRRSRVEIGVGSESKEPVVVAESSLRRQGSNRRSPLKSEAMNEGKELVAKTKTKPITSK 423

Query: 421 ----------VNSSTRRISL------------GIEVMKENKERVAAVTSPSRRSPVKIEV 480
                     V SST+  S             G  ++K    +  +V S      +    
Sbjct: 424 PKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLLKPKAFKEKSVISSGSSGNIAAHS 483

Query: 481 MNES---EGSRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKMR 540
            N S   EG+ T K  G ++    ++ +DP S+D V + PAIK KN++ VS+V +QNK R
Sbjct: 484 NNSSKSGEGAGTLKGNGMKVVEKSIITMDPNSVDTVVNLPAIK-KNSKAVSQVKSQNKTR 543

Query: 541 RAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANCS 600
           R   KE   VE QEK L+VIN ETK   ++ESD N+KHGL    K PSS      L N  
Sbjct: 544 RIQAKEASSVESQEKILHVINVETKKNKLVESDQNDKHGLKGYQKSPSS------LTNGP 603

Query: 601 SLSPPKEDCSGTKSTRSKANATSSGLNKQGGIKI---------GRSPRMLQTKGKDSSSL 660
           SLSP +ED  GTK T+ +ANAT SG  K GGIK          G+SPRMLQTKGKDSSS 
Sbjct: 604 SLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDLNGVKKGKSPRMLQTKGKDSSSF 663

Query: 661 SVSFKKGKAVDLHSENPSPTRLKFIRGRSFGDNEKSKDGQRTSFKKVVNKGISKDSIPSS 684
           S+SF+  K +DL S++  PTRLKF+ G+S GDN+KSKDG+RTS KK + KGISK+S P S
Sbjct: 664 SLSFRNKKVIDLDSKSHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGIVKGISKNSTPPS 723

BLAST of MC08g1910 vs. TAIR 10
Match: AT5G39380.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 99.0 bits (245), Expect = 1.6e-20
Identity = 109/318 (34.28%), Postives = 163/318 (51.26%), Query Frame = 0

Query: 376 KENKERVAA--VTSP--SRRSPVKIEVMNESEGSRTPKRIGTRMAGNQVVKLDPK-SIDA 435
           K N E+V A  V  P   +R+  K E     +G+ + +    +        L P+ S+  
Sbjct: 197 KVNHEKVVASPVLKPKMGKRNDGKDEDGKVKKGTVSSRVASKKAPVTPRASLSPRLSVRL 256

Query: 436 VGDSPAIKNKNTRVVSRVITQNKMRRAP------TKEVQIVEPQEKTLYVINTETKNTNV 495
            G S   K+++ +  S    QN+  R         K++     +EKTL+V+  ET N  V
Sbjct: 257 AGSSSLRKSQSLKAASSSSRQNQKPRPVNRTDEFNKQLDDYPVEEKTLHVVEMETTNNVV 316

Query: 496 MESDHNEKHGLNLRPKQPSSPYQSSSLANCSSLSPPKEDCSGTKSTRSKANATSSGLNKQ 555
            E+D N++ G       P  P QS+   +  ++S  +E    + S  +++     GL+  
Sbjct: 317 SENDQNQQ-GFVEPFLPPLPPTQSTPKDDECTVSETEEYEYTSGSNEAESEEEEIGLS-- 376

Query: 556 GGIKIGRSPRMLQTKGKDSSSLSVSFKKGKAVDLHSENPSPTRLKFIRGRSFGDNEKSKD 615
            G K  R+ R  +    D ++  + F++G  VD  +      +LKF RGR  G+ +K++D
Sbjct: 377 NGEKKPRAARK-EGDSADEAARKLRFRRGTIVDPDTVGEKARKLKFRRGRGLGE-DKAQD 436

Query: 616 GQ-RTSFKKVVNKGISKDSI-PSSEKVVLRHQNVQGKKDTQVLFNKVIAETAGKLVRNQK 675
            Q R SFKK   + I ++ +    EKVVLRHQ+VQ +KD Q LFN VI ETA KLV  +K
Sbjct: 437 AQVRRSFKK--REDIREEEVNEDGEKVVLRHQDVQ-EKDAQGLFNNVIEETASKLVEARK 496

Query: 676 GKVKALVGAFEKVISLQD 681
            KVKALVGAFE VISLQ+
Sbjct: 497 SKVKALVGAFETVISLQE 506

BLAST of MC08g1910 vs. TAIR 10
Match: AT5G15430.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 94.0 bits (232), Expect = 5.1e-19
Identity = 123/400 (30.75%), Postives = 189/400 (47.25%), Query Frame = 0

Query: 315 SPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVPVN-----SSTRR-- 374
           SPSRR    +   + +K +V   N SS  C V     + + ++VV V+      ST+R  
Sbjct: 96  SPSRRCD-SVGGFDHAKSQV--RNFSSGVCDVKKSHADGTNEKVVSVSESRLADSTKRKK 155

Query: 375 -------ISLG-IEVMKENKERVAAVTSPS---------RRSPVKIEVMNESEGSRTPKR 434
                  +S G  + + E K RV A+   +         RRS VK + MN    +   K+
Sbjct: 156 KKKKTVYVSRGRAKEIVEQKRRVTALKLKAVAQTAEIALRRSTVKRKKMNGGSKAAEQKK 215

Query: 435 IGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKMRRAPTKEVQIVEPQEK 494
                    V+ L   S+ + G S  +K K       V  +   +    K   +VE  EK
Sbjct: 216 --------AVMALRRASMSSKGCSRCLKTKKESNSLSVPLKKTRKHVGDKCKDLVE--EK 275

Query: 495 TLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSLANCSSLSPPKEDCSGTKST 554
           TLYVI  ET +  ++ES+ N++  ++     P S  +S     C   +  +++ S  +  
Sbjct: 276 TLYVIKMETVD-EIVESELNQRCVMDSPIDDPKSE-KSQDKGECIE-TEHEDESSQEEED 335

Query: 555 RSKANATSSGLNKQGGIKIGRSPRMLQTKGKDSSSLSVSFKKGKAVDLHSENPSPTRLKF 614
             + +   S    +   + G+S           +++ +  ++GK +D  SE  SP +LKF
Sbjct: 336 EEEEDENVSVSEDKNTTREGKSKAFSAESAITGNAMKLRIRRGKIIDFGSEGNSPRKLKF 395

Query: 615 IRGRSF-GDNEKSKDGQRTSFK-KVVNKGISKDSIPSSEKVVLRHQNVQGKKDTQV-LFN 674
            RG+   G +  SK G R   K K  N    K+      +VVL+HQ+ + K++++V LFN
Sbjct: 396 KRGKIISGADTTSKSGGRRRLKTKGTNLSNDKEQ-QRKPRVVLKHQDTEKKRESRVLLFN 455

Query: 675 KVIAETAGKLVRNQKGKVKALVGAFEKVISLQDKKPSVRT 688
           KVI ETA KLV+ +K KVKALVGAFE VISLQ+K  S  T
Sbjct: 456 KVIKETANKLVQTRKSKVKALVGAFESVISLQEKTSSATT 478


HSP 2 Score: 31.2 bits (69), Expect = 4.1e+00
Identity = 99/484 (20.45%), Postives = 189/484 (39.05%), Query Frame = 0

Query: 18  RRFSMGKASLSGIDDQI--SSRRSSTGSCHDFCKYGHKHSLETKARVPLLKRAMKKSLNG 77
           RR S GK S     +++  +  RS TGSCHD CKYG K   E K RVP  KR  +     
Sbjct: 19  RRISTGKLSFLYTQEKVVPNYLRSPTGSCHDACKYGRKDESEDKPRVPHRKRVSRSFSGA 78

Query: 78  QNSDLVVAMPKKGKPPPVPVTKLKTSPDLGTCITGGIDVVKRVV---------PVNSPAR 137
            N D     P + K     +TK   SP       GG D  K  V            S A 
Sbjct: 79  INLD----SPLRKK----ALTKPLLSPSRRCDSVGGFDHAKSQVRNFSSGVCDVKKSHAD 138

Query: 138 RSPVEIVDMNESKKHTVPVNSPTRRNSIGIEIMNENKERVAPVTSPSRR-SLVEIEVMNE 197
            +  ++V ++ES+     +   T+R     + +  ++ R   +    RR + ++++ + +
Sbjct: 139 GTNEKVVSVSESR-----LADSTKRKKKKKKTVYVSRGRAKEIVEQKRRVTALKLKAVAQ 198

Query: 198 SKEQVVPVNSSSRQSPAEIEVMNESKKCVVPVNSSTRQSSLGTEVMNENKE-RVAAVTSP 257
           + E  +  ++  R+         E KK V+ +  ++  S   +  +   KE    +V   
Sbjct: 199 TAEIALRRSTVKRKKMNGGSKAAEQKKAVMALRRASMSSKGCSRCLKTKKESNSLSVPLK 258

Query: 258 SRRSSVGVEVMNESKERVVPVNSSSRQSPVEIEVMNEGKKRVVPVNFSTRRSSLGPEVMN 317
             R  VG +  +  +E+ + V    +   V+  V +E  +R V        S +      
Sbjct: 259 KTRKHVGDKCKDLVEEKTLYV---IKMETVDEIVESELNQRCV------MDSPIDDPKSE 318

Query: 318 ENKERVAAATSPSRRNSVKIEAMNESKGRVVPINSSSRQCPVDIEVVNESKKRVVPVNSS 377
           +++++     +     S + E   E +   V ++        D     E K +     S+
Sbjct: 319 KSQDKGECIETEHEDESSQEEEDEEEEDENVSVSE-------DKNTTREGKSKAFSAESA 378

Query: 378 TRRISLGIEVMKENKERVAAVTSPSRRSPVK-------IEVMNESEGSRTPKRIGTRMAG 437
               ++ + + +       +  +  R+   K        +  ++S G R  K  GT ++ 
Sbjct: 379 ITGNAMKLRIRRGKIIDFGSEGNSPRKLKFKRGKIISGADTTSKSGGRRRLKTKGTNLSN 438

Query: 438 NQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVI--TQNKM---RRAPTKEV-----QIVEP 472
           ++  +  P+ +    D+   +     + ++VI  T NK+   R++  K +      ++  
Sbjct: 439 DKEQQRKPRVVLKHQDTEKKRESRVLLFNKVIKETANKLVQTRKSKVKALVGAFESVISL 473

BLAST of MC08g1910 vs. TAIR 10
Match: AT5G61260.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 87.0 bits (214), Expect = 6.2e-17
Identity = 95/302 (31.46%), Postives = 140/302 (46.36%), Query Frame = 0

Query: 399 MNESEGSRTPKRIGTRMAGNQVVKLDP-------KSIDAVGDSPAIKNKNTRVVSRVITQ 458
           +  ++ +R  K   + ++G+ VVK  P        +   VG S     KN + V +  T 
Sbjct: 195 VKSNDRARKNKETESGLSGSAVVKKVPALRTDKSSTSTGVGSSKVCAPKNLKNVEKAKT- 254

Query: 459 NKMRRAPTKEVQIVEPQEKTLYVINTETKNTNVMESDHNEKHGLNLRPKQPSSPYQSSSL 518
                  T+ +   + +EKT+ V+ +  K                   KQPSS  ++   
Sbjct: 255 -------TQTISGEDVKEKTVCVVESSVKGVK--------------SEKQPSSEKKTMKS 314

Query: 519 ANCSSLSPPKEDCSGTKSTRSKANATSSGLNK----------QGGIKIGRSPRMLQTKGK 578
            N S  + PK   S TK    K    S+GL K          +   K  +  R  +T  K
Sbjct: 315 GNKSLSTTPKRGSSPTKQIPGK---ISTGLTKKKETGSADVVEANPKPEKKVRPKKTGVK 374

Query: 579 DSSSLSVSFKKGKAVDLHSENPSPTRLKFIRGRSFGDNEKSKDGQRTSFK-KVVNKGISK 638
            S +  ++FKKGK +D   E+ SP  +KF + R   + +   +G++ + K + +      
Sbjct: 375 VSLAQQMTFKKGKVLDPKPEDSSPRWIKF-KKRVVQELKTQSEGKKKNLKDRRLGVETKT 434

Query: 639 DSIPSS--EKVVLRHQNVQGKKDTQVLFNKVIAETAGKLVRNQKGKVKALVGAFEKVISL 681
           DS   S  EKVVLRH+ V+GKK    LFN VI ET  KL + +K KVKAL+GAFE VISL
Sbjct: 435 DSCEGSKREKVVLRHRKVEGKKKMITLFNNVIEETVNKLTKVRKHKVKALIGAFETVISL 470

BLAST of MC08g1910 vs. TAIR 10
Match: AT5G07820.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 78.6 bits (192), Expect = 2.2e-14
Identity = 118/431 (27.38%), Postives = 184/431 (42.69%), Query Frame = 0

Query: 286 VVPVNFSTRRSSLGPEVMNENKERVAAATSPSRRNSVKIEAMNESKGRVV-PINSSSRQC 345
           V PV  + ++++L   V+N+   R++   SP    S  ++  N+ KG++V P+       
Sbjct: 167 VSPVVRTVKKTNL---VVNK-ASRISQNKSPKEDLSKNLK--NKEKGKIVEPVRCDDVLE 226

Query: 346 PVDIEVVNESKKRVVPVNSSTRRISLGIEVMKENKERVAAVTSPSRRSPVKIEVMNESEG 405
             D+EV   S+   +  N S++  +L      +NKE+ A +  P R   V          
Sbjct: 227 KTDLEVKKVSR---ISENKSSKEDTL------KNKEK-AKIDEPVRCDDV---------- 286

Query: 406 SRTPKRIGTRMAGNQVVKLDPKSIDAVGDSPAIKNKNTRVVSRVITQNKMRRAPTKEVQI 465
                             L+  S+DA   S   +NKN++       +NK +    + V+ 
Sbjct: 287 ------------------LEKTSLDAQKVSRISENKNSKEERLKNLKNKEKTNIDEPVRP 346

Query: 466 VEPQEKTLYVINT---------ETKNTNVMESDHNE---------KHGLNLRPKQP---- 525
            +  EKTLYV+ +          TK+  + E+  +          K  L+L P  P    
Sbjct: 347 DDAVEKTLYVVESSVEKKKKKMSTKSVKISETQQSSEKKIIRSTGKKSLSLLPSLPPPSE 406

Query: 526 ----SSPYQSSSLANCSSLSPPKEDCSGTKSTRSKANATSSGLNKQGGIKIGRSPRMLQT 585
               S P       + S  S P++  SG+ +  +     S    K+ G+K+   P     
Sbjct: 407 VVTGSDPRPIRQTTSRSKTSLPEKKQSGSANLVTNPKPESKIRPKRIGLKVTPPP----- 466

Query: 586 KGKDSSSLSVSFKKGKAVDLHSENPSPTRLKF---------IRGRSFGDNEKSKDGQRTS 645
                +   ++FKKGK ++   E+ + T +KF         +R       +KS   +R  
Sbjct: 467 ---PPTKQQMNFKKGKVLEPKPEDSTTTSIKFKKIVVQEPKLRTSDVNKKKKSLKDKREG 526

Query: 646 FKKVVNKGISKDSIPSSEKVVLRHQNVQGKKDTQVLFNKVIAETAGKLVRNQKGKVKALV 681
             K+  +G         EKVVLRH+ V+ KK  Q LFN VI ET  KL   +K KVKALV
Sbjct: 527 VGKINGEG-------KREKVVLRHRKVEVKKKLQTLFNNVIEETVNKLEEVRKSKVKALV 538

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022144538.10.076.93flocculation protein FLO11-like isoform X1 [Momordica charantia] >XP_022144539.1... [more]
XP_022144540.10.076.85flocculation protein FLO11-like isoform X2 [Momordica charantia][more]
KAG6607113.13.90e-19549.87hypothetical protein SDJN03_00455, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022949115.15.89e-19448.93muscle M-line assembly protein unc-89-like isoform X2 [Cucurbita moschata][more]
XP_023523723.19.75e-19448.38uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo] >XP_023523724.... [more]
Match NameE-valueIdentityDescription
A0A6J1CSL10.076.93flocculation protein FLO11-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1CRX00.076.85flocculation protein FLO11-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1GBV92.85e-19448.93muscle M-line assembly protein unc-89-like isoform X2 OS=Cucurbita moschata OX=3... [more]
A0A6J1GB512.05e-19147.20muscle M-line assembly protein unc-89-like isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1K8S11.32e-19048.84muscle M-line assembly protein unc-89-like isoform X5 OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
AT5G39380.11.6e-2034.28Plant calmodulin-binding protein-related [more]
AT5G15430.15.1e-1930.75Plant calmodulin-binding protein-related [more]
AT5G61260.16.2e-1731.46Plant calmodulin-binding protein-related [more]
AT5G07820.12.2e-1427.38Plant calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 564..677
e-value: 2.3E-23
score: 93.6
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 569..677
e-value: 1.3E-27
score: 96.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 500..545
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 488..569
NoneNo IPR availablePANTHERPTHR33349:SF1EMB|CAB62594.1coord: 261..684
coord: 10..265
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 261..684
coord: 10..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1910.1MC08g1910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding