MC08g1904 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1904 vs. ExPASy Swiss-Prot
Match: P53536 (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Vicia faba OX=3906 GN=PHO1 PE=2 SV=2)

HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 753/1055 (71.37%), Postives = 858/1055 (81.33%), Query Frame = 0

Query: 35   KSKLLLVRTS--NWRSARR--------TFAVK-NVSSEPKQKLKDDPVADEESSIVASAF 94
            +S  L VRT+   +RS +R         F+VK    +E KQK+KD  V  +E+    S+F
Sbjct: 32   RSSSLFVRTNVIKYRSVKRNLEFRRRSAFSVKCGSGNEAKQKVKDQEV-QQEAKTSPSSF 91

Query: 95   TPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDALIINWNATYELYEKLNVK 154
             PD  SI SSIKYHAEFTPLFSP++F+LP+AF ATAQSVRDALIINWNATY+ YEKLNVK
Sbjct: 92   APDTTSIVSSIKYHAEFTPLFSPEKFELPQAFIATAQSVRDALIINWNATYDYYEKLNVK 151

Query: 155  QAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVACQEPDAALGNGGLGRLA 214
            QAYYLSMEFLQGRALLNAIGNLELTG YAEALS+L Y+LE+VA QEPDAALGNGGLGRLA
Sbjct: 152  QAYYLSMEFLQGRALLNAIGNLELTGPYAEALSQLSYKLEDVAHQEPDAALGNGGLGRLA 211

Query: 215  SCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWLEIGNPWEVVRNDIKYPI 274
            SCFLDSLATLNYPAWGYGLRY+YGLFKQ+ITKDGQEEVAE+WLE+GNPWE+VRND+ YP+
Sbjct: 212  SCFLDSLATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLEMGNPWEIVRNDVSYPV 271

Query: 275  KFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLRLWSTKAPTEDFDLSAFN 334
            +FYGKVV GSDGKK+W GGEDI+AVA+DVPIPGYKT++TINLRLWSTKA +E+FDL+AFN
Sbjct: 272  RFYGKVVSGSDGKKHWVGGEDIKAVAHDVPIPGYKTRSTINLRLWSTKAASEEFDLNAFN 331

Query: 335  AGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLKLIIEFLVRKIENFMKRN 394
            +G HT+ASEALA+A+K                                            
Sbjct: 332  SGRHTEASEALANAEK-------------------------------------------- 391

Query: 395  HLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQ 454
                                                   IC++LYPGD+SIEGK LRLKQ
Sbjct: 392  ---------------------------------------ICYILYPGDESIEGKTLRLKQ 451

Query: 455  QYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLS 514
            QYTLCSASLQDI+ARFERRSGA+  WE+FPEKVAVQMNDTHPTLCIPELMRIL+D+KGLS
Sbjct: 452  QYTLCSASLQDIIARFERRSGASVNWEDFPEKVAVQMNDTHPTLCIPELMRILIDIKGLS 511

Query: 515  WEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGT 574
            W++AW ITQRTVAYTNHTVLPEALEKWS++LM++LLPRHVEIIE IDEELI+TII+EYGT
Sbjct: 512  WKDAWNITQRTVAYTNHTVLPEALEKWSMDLMEKLLPRHVEIIEMIDEELIRTIIAEYGT 571

Query: 575  ADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVAST---------EEPEISNEVD 634
            AD  LL KKLKE+RILENV+LPA ++D++++ +E++ ++S          EE E S E  
Sbjct: 572  ADSDLLDKKLKEMRILENVELPAEFADILVKTKEATDISSEEVQISKEGGEEEETSKEGG 631

Query: 635  SADEENSEGQAKSIGED-------ETVEEEDEPESKGIQDKK---VEPIPPPPKMVRMAN 694
              +EE   G  +  G+D       + + E+D      I DKK    EP+P PPK+VRMAN
Sbjct: 632  EEEEEKEVGGGREEGDDGKEDEVEKAIAEKDGTVKSSIGDKKKKLPEPVPVPPKLVRMAN 691

Query: 695  LCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKLI 754
            LCV GGHAVNGVAEIHSEIVKD+VFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSK+I
Sbjct: 692  LCVVGGHAVNGVAEIHSEIVKDDVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKII 751

Query: 755  TNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAM 814
            T WIG+EDW+LNTEKL EL+ FADNEDLQTQWR AKRNNK+K  +FL+E+TGY+VSPD+M
Sbjct: 752  TQWIGTEDWILNTEKLAELRKFADNEDLQTQWREAKRNNKVKVAAFLRERTGYSVSPDSM 811

Query: 815  FDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKR 874
            FDIQVKRIHEYKRQLLNI GIVYRYKKMKEM+A ERKE FVPRVCIFGGKAFATYVQAKR
Sbjct: 812  FDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMNAAERKENFVPRVCIFGGKAFATYVQAKR 871

Query: 875  IVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSN 934
            IVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAE+LIPASELSQHISTAGMEASGTSN
Sbjct: 872  IVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAEMLIPASELSQHISTAGMEASGTSN 931

Query: 935  MKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEE 994
            MKFAMNGC+ IGTLDGANVEIR+EVGA+NFFLFGAKA EI GLRKERA GKF+PDPRFEE
Sbjct: 932  MKFAMNGCLQIGTLDGANVEIREEVGADNFFLFGAKAREIVGLRKERARGKFVPDPRFEE 991

Query: 995  VKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKW 1054
            VK++VRSGVFGSY+Y+E+IGSLEGNEGFGRADYFLVG+DFPSY+ECQE+VD+AYRDQKKW
Sbjct: 992  VKKFVRSGVFGSYNYDELIGSLEGNEGFGRADYFLVGQDFPSYLECQEEVDKAYRDQKKW 1002

Query: 1055 TRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVEL 1060
            TRMSILNTAGS KFSSDRTIHEYA++IWNI+PV+L
Sbjct: 1052 TRMSILNTAGSSKFSSDRTIHEYAREIWNIEPVKL 1002

BLAST of MC08g1904 vs. ExPASy Swiss-Prot
Match: P04045 (Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 739/1053 (70.18%), Postives = 837/1053 (79.49%), Query Frame = 0

Query: 12   NRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSSEPKQKLKDDPV 71
            N A  F+H +S    +  + +   SKL L +TS++R  +R F V N  SE        P+
Sbjct: 5    NGAHLFNHYSSNSRFIHFTSRNTSSKLFLTKTSHFRRPKRCFHVNNTLSEKIH----HPI 64

Query: 72   ADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDALIINWN 131
             ++      S+F PDAASI SSIKYHAEFTP+FSP+RF+LPKAFFATAQSVRD+L+INWN
Sbjct: 65   TEQGGESDLSSFAPDAASITSSIKYHAEFTPVFSPERFELPKAFFATAQSVRDSLLINWN 124

Query: 132  ATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVACQEPD 191
            ATY++YEKLN+KQAYYLSMEFLQGRALLNAIGNLELTGA+AEAL  LG+ LENVA QEPD
Sbjct: 125  ATYDIYEKLNMKQAYYLSMEFLQGRALLNAIGNLELTGAFAEALKNLGHNLENVASQEPD 184

Query: 192  AALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWLEIGNP 251
            AALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQ+ITKDGQEEVAE+WLEIG+P
Sbjct: 185  AALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLEIGSP 244

Query: 252  WEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLRLWSTK 311
            WEVVRND+ YPIKFYGKV  GSDGK+ W GGEDI+AVAYDVPIPGYKT+ TI+LRLWST+
Sbjct: 245  WEVVRNDVSYPIKFYGKVSTGSDGKRYWIGGEDIKAVAYDVPIPGYKTRTTISLRLWSTQ 304

Query: 312  APTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLKLIIEF 371
             P+ DFDLSAFNAGEHTKA EA A+A+K                                
Sbjct: 305  VPSADFDLSAFNAGEHTKACEAQANAEK-------------------------------- 364

Query: 372  LVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYLLQICHVLYPGD 431
                                                               IC++LYPGD
Sbjct: 365  ---------------------------------------------------ICYILYPGD 424

Query: 432  DSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPE 491
            +S EGKILRLKQQYTLCSASLQDI++RFERRSG   KWEEFPEKVAVQMNDTHPTLCIPE
Sbjct: 425  ESEEGKILRLKQQYTLCSASLQDIISRFERRSGDRIKWEEFPEKVAVQMNDTHPTLCIPE 484

Query: 492  LMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDE 551
            LMRIL+DLKGL+W EAW ITQRTVAYTNHTVLPEALEKWS ELMQ+LLPRHVEIIE IDE
Sbjct: 485  LMRILIDLKGLNWNEAWNITQRTVAYTNHTVLPEALEKWSYELMQKLLPRHVEIIEAIDE 544

Query: 552  ELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISN 611
            EL+  I+ +YG+ DL  L +KL  +RILEN DLP++ ++L I+P E SV   TE  E+ +
Sbjct: 545  ELVHEIVLKYGSMDLNKLEEKLTTMRILENFDLPSSVAELFIKP-EISVDDDTETVEVHD 604

Query: 612  EVDSAD-----EENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMVRMANLC 671
            +V+++D     +E+  G+  S+  +   E++ + ++        EP   PPK VRMANLC
Sbjct: 605  KVEASDKVVTNDEDDTGKKTSVKIEAAAEKDIDKKT----PVSPEPAVIPPKKVRMANLC 664

Query: 672  VAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKLITN 731
            V GGHAVNGVAEIHSEIVK+EVFN FY+LWPEKFQNKTNGVTPRRWIRFCNP LS +IT 
Sbjct: 665  VVGGHAVNGVAEIHSEIVKEEVFNDFYELWPEKFQNKTNGVTPRRWIRFCNPPLSAIITK 724

Query: 732  WIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFD 791
            W G+EDWVL TEKL EL+ FADNEDLQ +WR AKR+NK+K VSFLKEKTGY+V PDAMFD
Sbjct: 725  WTGTEDWVLKTEKLAELQKFADNEDLQNEWREAKRSNKIKVVSFLKEKTGYSVVPDAMFD 784

Query: 792  IQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIV 851
            IQVKRIHEYKRQLLNI GIVYRYKKMKEM+A ERK  FVPRVCIFGGKAFATYVQAKRIV
Sbjct: 785  IQVKRIHEYKRQLLNIFGIVYRYKKMKEMTAAERKTNFVPRVCIFGGKAFATYVQAKRIV 844

Query: 852  KFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMK 911
            KFITDVGAT+NHDPEIGDLLKV+FVPDYNVSVAELLIPAS+LS+HISTAGMEASGTSNMK
Sbjct: 845  KFITDVGATINHDPEIGDLLKVVFVPDYNVSVAELLIPASDLSEHISTAGMEASGTSNMK 904

Query: 912  FAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVK 971
            FAMNGCI IGTLDGANVEIR+EVG ENFFLFGA+AHEIAGLRKERA+GKF+PD RFEEVK
Sbjct: 905  FAMNGCIQIGTLDGANVEIREEVGEENFFLFGAQAHEIAGLRKERADGKFVPDERFEEVK 964

Query: 972  EYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTR 1031
            E+VRSG FGSY+Y+++IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WT 
Sbjct: 965  EFVRSGAFGSYNYDDLIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTT 965

Query: 1032 MSILNTAGSYKFSSDRTIHEYAKDIWNIKPVEL 1060
            MSILNTAGSYKFSSDRTIHEYAKDIWNI+ VE+
Sbjct: 1025 MSILNTAGSYKFSSDRTIHEYAKDIWNIEAVEI 965

BLAST of MC08g1904 vs. ExPASy Swiss-Prot
Match: P27598 (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Ipomoea batatas OX=4120 PE=2 SV=1)

HSP 1 Score: 1451.0 bits (3755), Expect = 0.0e+00
Identity = 729/1019 (71.54%), Postives = 825/1019 (80.96%), Query Frame = 0

Query: 42   RTSNWRSARRTFAVKNVSSEPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFT 101
            RT+  +  +RT  VK V  E KQ ++       E +++      DAASIASSIKYHAEF+
Sbjct: 30   RTAGLQRTKRTLLVKCVLDETKQTIQHVVTEKNEGTLL------DAASIASSIKYHAEFS 89

Query: 102  PLFSPDRFDLPKAFFATAQSVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNA 161
            P FSP+RF+LPKA+FATAQSVRDALI+NWNATY+ YEKLN+KQAYYLSMEFLQGRALLNA
Sbjct: 90   PAFSPERFELPKAYFATAQSVRDALIVNWNATYDYYEKLNMKQAYYLSMEFLQGRALLNA 149

Query: 162  IGNLELTGAYAEALSKLGYELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYG 221
            IGNLELTG YAEAL+KLG+ LENVA +EPDAALGNGGLGRLASCFLDSLATLNYPAWGYG
Sbjct: 150  IGNLELTGEYAEALNKLGHNLENVASKEPDAALGNGGLGRLASCFLDSLATLNYPAWGYG 209

Query: 222  LRYRYGLFKQKITKDGQEEVAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTG 281
            LRY+YGLFKQ+ITKDGQEEVAE+WLE+GNPWE++R D+ YP+KF+GKV+ GSDGKK+W G
Sbjct: 210  LRYKYGLFKQRITKDGQEEVAEDWLELGNPWEIIRMDVSYPVKFFGKVITGSDGKKHWIG 269

Query: 282  GEDIEAVAYDVPIPGYKTKNTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQ 341
            GEDI AVAYDVPIPGYKT+ TI+LRLWSTK P+EDFDL +FNAGEHTKA EA A+A+K  
Sbjct: 270  GEDILAVAYDVPIPGYKTRTTISLRLWSTKVPSEDFDLYSFNAGEHTKACEAQANAEK-- 329

Query: 342  LLDCTNSLLWFQLSLLKCIGLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKK 401
                                                                        
Sbjct: 330  ------------------------------------------------------------ 389

Query: 402  GPESSVCTLKFYKNGSSYLLQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFER 461
                                 IC++LYPGD+SIEGKILRLKQQYTLCSASLQDI+ARFER
Sbjct: 390  ---------------------ICYILYPGDESIEGKILRLKQQYTLCSASLQDIIARFER 449

Query: 462  RSGANKKWEEFPEKVAVQMNDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHT 521
            RSG   KWEEFPEKVAVQMNDTHPTLCIPEL+RIL+DLKGLSW+EAW ITQRTVAYTNHT
Sbjct: 450  RSGEYVKWEEFPEKVAVQMNDTHPTLCIPELIRILIDLKGLSWKEAWNITQRTVAYTNHT 509

Query: 522  VLPEALEKWSLELMQQLLPRHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILEN 581
            VLPEALEKWS ELM++LLPRH+EIIE IDE+LI  I+SEYGT+DL +L KKL ++RILEN
Sbjct: 510  VLPEALEKWSYELMEKLLPRHIEIIEMIDEQLINEIVSEYGTSDLDMLEKKLNDMRILEN 569

Query: 582  VDLPAAYSDLIIEPEESSVVASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPE 641
             D+P++ ++L  +P+E+S+V  +EE E+S +V +   E S+       +DE  E++ E E
Sbjct: 570  FDIPSSIANLFTKPKETSIVDPSEEVEVSGKVVTESVEVSDKVVTESEKDELEEKDTELE 629

Query: 642  SKGIQDKKVEPIPPPPKMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQ 701
                +D+   P P PPKMVRMANLCV GGHAVNGVAEIHS+IVK++VFN FY+LWPEKFQ
Sbjct: 630  ----KDEDPVPAPIPPKMVRMANLCVVGGHAVNGVAEIHSDIVKEDVFNDFYQLWPEKFQ 689

Query: 702  NKTNGVTPRRWIRFCNPDLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKR 761
            NKTNGVTPRRWIRFCNP LS +IT WIG+EDWVLNTEKL EL+ FADNEDLQ +WR AKR
Sbjct: 690  NKTNGVTPRRWIRFCNPALSNIITKWIGTEDWVLNTEKLAELRKFADNEDLQIEWRAAKR 749

Query: 762  NNKLKAVSFLKEKTGYTVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERK 821
            +NK+K  SFLKE+TGY+VSP+AMFDIQVKRIHEYKRQLLNILGIVYRYK+MKEMSA ER+
Sbjct: 750  SNKVKVASFLKERTGYSVSPNAMFDIQVKRIHEYKRQLLNILGIVYRYKQMKEMSARERE 809

Query: 822  EAFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAEL 881
              FVPRVCIFGGKAFATYVQAKRI KFITDVGAT+NHDPEIGDLLKVIFVPDYNVS AEL
Sbjct: 810  AKFVPRVCIFGGKAFATYVQAKRIAKFITDVGATINHDPEIGDLLKVIFVPDYNVSAAEL 869

Query: 882  LIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKA 941
            LIPAS LSQHISTAGMEASG SNMKFAMNGCILIGTLDGANVEIRQEVG ENFFLFGA+A
Sbjct: 870  LIPASGLSQHISTAGMEASGQSNMKFAMNGCILIGTLDGANVEIRQEVGEENFFLFGAEA 929

Query: 942  HEIAGLRKERAEGKFIPDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVG 1001
            HEIAGLRKERAEGKF+PD RFEEVKE+++ GVFGS  Y+E++GSLEGNEGFGR DYFLVG
Sbjct: 930  HEIAGLRKERAEGKFVPDERFEEVKEFIKRGVFGSNTYDELLGSLEGNEGFGRGDYFLVG 955

Query: 1002 KDFPSYIECQEQVDEAYRDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1061
            KDFPSYIECQE+VDEAYRDQK WTRMSILNTAGSYKFSSDRTIHEYAKDIWNI+PV  P
Sbjct: 990  KDFPSYIECQEKVDEAYRDQKIWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIQPVVFP 955

BLAST of MC08g1904 vs. ExPASy Swiss-Prot
Match: Q9LIB2 (Alpha-glucan phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=PHS1 PE=1 SV=1)

HSP 1 Score: 1449.1 bits (3750), Expect = 0.0e+00
Identity = 742/1054 (70.40%), Postives = 838/1054 (79.51%), Query Frame = 0

Query: 14   ADSFSHANSFPTLLGLSHKYGK--SKLLLVRTSNWRSA--RRTF-AVKNVSSEPKQKLKD 73
            A+     NS  +L+      GK  +++   R  + R +  RR+F +VK++SSEPK K+ D
Sbjct: 13   AEVLIQCNSLSSLVSRRCDDGKWRTRMFPARNRDLRPSPTRRSFLSVKSISSEPKAKVTD 72

Query: 74   DPVADEESSIVAS--AFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDAL 133
              +  E+   ++S   F PDAAS+ASSIKYHAEFTPLFSP++F+LPKAFFATAQSVRDAL
Sbjct: 73   AVLDSEQEVFISSMNPFAPDAASVASSIKYHAEFTPLFSPEKFELPKAFFATAQSVRDAL 132

Query: 134  IINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVA 193
            I+NWNATYE Y ++NVKQAYYLSMEFLQGRAL NA+GNL L  AY +AL +LG++LE+VA
Sbjct: 133  IMNWNATYEYYNRVNVKQAYYLSMEFLQGRALSNAVGNLGLNSAYGDALKRLGFDLESVA 192

Query: 194  CQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWL 253
             QEPD ALGNGGLGRLASCFLDS+ATLNYPAWGYGLRY+YGLFKQ+ITKDGQEE AE+WL
Sbjct: 193  SQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEAAEDWL 252

Query: 254  EIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLR 313
            E+ NPWE+VRND+ YPIKFYGKVV GSDGKK W GGEDI AVAYDVPIPGYKTK TINLR
Sbjct: 253  ELSNPWEIVRNDVSYPIKFYGKVVFGSDGKKRWIGGEDIVAVAYDVPIPGYKTKTTINLR 312

Query: 314  LWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLK 373
            LWSTKAP+EDFDLS++N+G+HT+A+EAL +A+K                           
Sbjct: 313  LWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEK--------------------------- 372

Query: 374  LIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYLLQICHV 433
                                                                    IC V
Sbjct: 373  --------------------------------------------------------ICFV 432

Query: 434  LYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPT 493
            LYPGD+S EGK LRLKQQYTLCSASLQDIVARFE RSG N  WEEFPEKVAVQMNDTHPT
Sbjct: 433  LYPGDESTEGKALRLKQQYTLCSASLQDIVARFETRSGGNVNWEEFPEKVAVQMNDTHPT 492

Query: 494  LCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEII 553
            LCIPELMRIL+DLKGLSWE+AWKITQRTVAYTNHTVLPEALEKWSLELM++LLPRHVEII
Sbjct: 493  LCIPELMRILMDLKGLSWEDAWKITQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEII 552

Query: 554  EQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEE 613
            E+IDEEL++TI+SEYGTAD  LL +KLK +RILENV+LP+A++D+I++P    V A    
Sbjct: 553  EKIDEELVRTIVSEYGTADPDLLEEKLKAMRILENVELPSAFADVIVKPVNKPVTAK--- 612

Query: 614  PEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMVRMANLC 673
             +  N V +  EE      K+ GE    EEEDE           EP   PPKMVRMANL 
Sbjct: 613  -DAQNGVKTEQEEE-----KTAGE----EEEDEVIP--------EPTVEPPKMVRMANLA 672

Query: 674  VAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKLITN 733
            V GGHAVNGVAEIHSEIVK +VFN F +LWPEKFQNKTNGVTPRRWIRFCNP LS +ITN
Sbjct: 673  VVGGHAVNGVAEIHSEIVKQDVFNDFVQLWPEKFQNKTNGVTPRRWIRFCNPYLSDIITN 732

Query: 734  WIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFD 793
            WIG+EDWVLNTEK+ EL+ FADNEDLQ++WR AK+ NKLK VS +KE+TGYTVSPDAMFD
Sbjct: 733  WIGTEDWVLNTEKVAELRKFADNEDLQSEWRAAKKKNKLKVVSLIKERTGYTVSPDAMFD 792

Query: 794  IQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIV 853
            IQ+KRIHEYKRQLLNILGIVYRYKKMKEMSA ER++AFVPRVCIFGGKAFATYVQAKRIV
Sbjct: 793  IQIKRIHEYKRQLLNILGIVYRYKKMKEMSASEREKAFVPRVCIFGGKAFATYVQAKRIV 852

Query: 854  KFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMK 913
            KFITDV +T+NHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMK
Sbjct: 853  KFITDVASTINHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMK 912

Query: 914  FAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVK 973
            F+MNGC+LIGTLDGANVEIR+EVG ENFFLFGAKA +I  LRKERAEGKF+PDP FEEVK
Sbjct: 913  FSMNGCVLIGTLDGANVEIREEVGEENFFLFGAKADQIVNLRKERAEGKFVPDPTFEEVK 962

Query: 974  EYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTR 1033
            ++V SGVFGS  Y+E+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WTR
Sbjct: 973  KFVGSGVFGSNSYDELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTR 962

Query: 1034 MSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1061
            MSI+NTAGS+KFSSDRTIHEYAKDIWNIK VELP
Sbjct: 1033 MSIMNTAGSFKFSSDRTIHEYAKDIWNIKQVELP 962

BLAST of MC08g1904 vs. ExPASy Swiss-Prot
Match: P53535 (Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 GN=STP-1 PE=1 SV=1)

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 713/1054 (67.65%), Postives = 832/1054 (78.94%), Query Frame = 0

Query: 18   SHANSFPTLLGLSHKY--GKSKLLLVRTS----NWRSARRTFAVKNVSSEPKQKLKDDPV 77
            S  NS  ++   ++ +    S +LL R      ++R  RR+F+V +V+S+ KQK KD   
Sbjct: 7    SGLNSISSISSFNNNFRSKNSNILLSRRRILLFSFRRRRRSFSVSSVASDQKQKTKD--- 66

Query: 78   ADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDALIINWN 137
            +  +       F PD+ S+ SSIKYHAEFTP FSP++F+LPKA++ATA+SVRD LIINWN
Sbjct: 67   SSSDEGFTLDVFQPDSTSVLSSIKYHAEFTPSFSPEKFELPKAYYATAESVRDTLIINWN 126

Query: 138  ATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVACQEPD 197
            ATYE YEK+NVKQAYYLSMEFLQGRALLNAIGNL LTG YA+AL+KLGY LE+VA QEPD
Sbjct: 127  ATYEFYEKMNVKQAYYLSMEFLQGRALLNAIGNLGLTGPYADALTKLGYSLEDVARQEPD 186

Query: 198  AALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWLEIGNP 257
            AALGNGGLGRLASCFLDS+ATLNYPAWGYGLRY+YGLFKQ ITKDGQEEVAENWLE+GNP
Sbjct: 187  AALGNGGLGRLASCFLDSMATLNYPAWGYGLRYQYGLFKQLITKDGQEEVAENWLEMGNP 246

Query: 258  WEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLRLWSTK 317
            WE+VRNDI YP+KFYGKV+ G+DG+K W GGEDI AVAYDVPIPGYKTK TINLRLW+TK
Sbjct: 247  WEIVRNDISYPVKFYGKVIEGADGRKEWAGGEDITAVAYDVPIPGYKTKTTINLRLWTTK 306

Query: 318  APTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLKLIIEF 377
               E FDL AFN G+H KA EA   A+K                                
Sbjct: 307  LAAEAFDLYAFNNGDHAKAYEAQKKAEK-------------------------------- 366

Query: 378  LVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYLLQICHVLYPGD 437
                                                               IC+VLYPGD
Sbjct: 367  ---------------------------------------------------ICYVLYPGD 426

Query: 438  DSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPTLCIPE 497
            +S+EGK LRLKQQYTLCSASLQDI+ARFE+RSG    W++FPEKVAVQMNDTHPTLCIPE
Sbjct: 427  ESLEGKTLRLKQQYTLCSASLQDIIARFEKRSGNAVNWDQFPEKVAVQMNDTHPTLCIPE 486

Query: 498  LMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDE 557
            L+RIL+D+KGLSW++AW+ITQRTVAYTNHTVLPEALEKWS  L+ +LLPRHVEII  IDE
Sbjct: 487  LLRILMDVKGLSWKQAWEITQRTVAYTNHTVLPEALEKWSFTLLGELLPRHVEIIAMIDE 546

Query: 558  ELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV---VASTEEPE 617
            EL+ TI++EYGT DL LL +KL ++RIL+NV++P++  +L+I+ EES+     A+ EE E
Sbjct: 547  ELLHTILAEYGTEDLDLLQEKLNQMRILDNVEIPSSVLELLIKAEESAADVEKAADEEQE 606

Query: 618  ISNEVDSADEENSEGQAKSIGEDETVE-EEDEPESKGIQDKKV-EPIPPPPKMVRMANLC 677
               + DS DEE    +A++  E+E  E ++ E E    + K++  P P  P++V MANLC
Sbjct: 607  EEGKDDSKDEETEAVKAETTNEEEETEVKKVEVEDSQAKIKRIFGPHPNKPQVVHMANLC 666

Query: 678  VAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKLITN 737
            V  GHAVNGVAEIHSEIVKDEVFN FYKLWPEKFQNKTNGVTPRRW+ FCNP+LS++IT 
Sbjct: 667  VVSGHAVNGVAEIHSEIVKDEVFNEFYKLWPEKFQNKTNGVTPRRWLSFCNPELSEIITK 726

Query: 738  WIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFD 797
            W GS+DW++NTEKL EL+ FADNE+LQ++WR AK NNK+K VS +KEKTGY VSPDAMFD
Sbjct: 727  WTGSDDWLVNTEKLAELRKFADNEELQSEWRKAKGNNKMKIVSLIKEKTGYVVSPDAMFD 786

Query: 798  IQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIV 857
            +Q+KRIHEYKRQLLNI GIVYRYKKMKEMS EERKE FVPRVCIFGGKAFATYVQAKRIV
Sbjct: 787  VQIKRIHEYKRQLLNIFGIVYRYKKMKEMSPEERKEKFVPRVCIFGGKAFATYVQAKRIV 846

Query: 858  KFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMK 917
            KFITDVG TVNHDPEIGDLLKV+FVPDYNVSVAE+LIP SELSQHISTAGMEASGTSNMK
Sbjct: 847  KFITDVGETVNHDPEIGDLLKVVFVPDYNVSVAEVLIPGSELSQHISTAGMEASGTSNMK 906

Query: 918  FAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVK 977
            F+MNGC+LIGTLDGANVEIR+EVG +NFFLFGA+AHEIAGLRKERAEGKF+PDPRFEEVK
Sbjct: 907  FSMNGCLLIGTLDGANVEIREEVGEDNFFLFGAQAHEIAGLRKERAEGKFVPDPRFEEVK 966

Query: 978  EYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTR 1037
             ++R+GVFG+Y+YEE++GSLEGNEG+GRADYFLVGKDFP YIECQ++VDEAYRDQKKWT+
Sbjct: 967  AFIRTGVFGTYNYEELMGSLEGNEGYGRADYFLVGKDFPDYIECQDKVDEAYRDQKKWTK 974

Query: 1038 MSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1061
            MSILNTAGS+KFSSDRTIH+YA+DIW I+PVELP
Sbjct: 1027 MSILNTAGSFKFSSDRTIHQYARDIWRIEPVELP 974

BLAST of MC08g1904 vs. NCBI nr
Match: XP_022144477.1 (alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic [Momordica charantia])

HSP 1 Score: 1906 bits (4937), Expect = 0.0
Identity = 977/1060 (92.17%), Postives = 977/1060 (92.17%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS
Sbjct: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY
Sbjct: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE
Sbjct: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADK                     
Sbjct: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADK--------------------- 360

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM
Sbjct: 421  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP
Sbjct: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV
Sbjct: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMV 660
            VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMV
Sbjct: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMV 660

Query: 661  RMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDL 720
            RMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDL
Sbjct: 661  RMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDL 720

Query: 721  SKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVS 780
            SKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVS
Sbjct: 721  SKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVS 780

Query: 781  PDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYV 840
            PDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYV
Sbjct: 781  PDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYV 840

Query: 841  QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS 900
            QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS
Sbjct: 841  QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS 900

Query: 901  GTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDP 960
            GTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDP
Sbjct: 901  GTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDP 960

Query: 961  RFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD 1020
            RFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD
Sbjct: 961  RFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD 977

Query: 1021 QKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            QKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP
Sbjct: 1021 QKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 977

BLAST of MC08g1904 vs. NCBI nr
Match: XP_038895156.1 (alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic [Benincasa hispida])

HSP 1 Score: 1725 bits (4468), Expect = 0.0
Identity = 888/1060 (83.77%), Postives = 922/1060 (86.98%), Query Frame = 0

Query: 3    ASSQLTVNLNRADSFSHANSFPT-LLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSSE 62
            A+SQ TV LNRADSFSH+NSFP  L+GLS +Y +SKLLLV TS WRS +RTF VKNVSSE
Sbjct: 2    ATSQFTVALNRADSFSHSNSFPPPLIGLSSRYRQSKLLLVPTSTWRSPKRTFLVKNVSSE 61

Query: 63   PKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQS 122
            PK K   DPVADEES I ASAF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQS
Sbjct: 62   PKLK---DPVADEESPIAASAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQS 121

Query: 123  VRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYE 182
            VRDALIINWN TYELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG+YAEAL+KLGYE
Sbjct: 122  VRDALIINWNETYELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGSYAEALNKLGYE 181

Query: 183  LENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEV 242
            LENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEEV
Sbjct: 182  LENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEEV 241

Query: 243  AENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKN 302
            AENWLEIGNPWE+ RNDIKY IKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKN
Sbjct: 242  AENWLEIGNPWEIFRNDIKYHIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKN 301

Query: 303  TINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIG 362
            TINLRLWSTKAPTEDFDLSAFNAGEHT+ASEALA+A+K                      
Sbjct: 302  TINLRLWSTKAPTEDFDLSAFNAGEHTRASEALANAEK---------------------- 361

Query: 363  LKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYLL 422
                                                                        
Sbjct: 362  ------------------------------------------------------------ 421

Query: 423  QICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMN 482
             ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVA FERRSG NKKWEEFPEKVAVQMN
Sbjct: 422  -ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVACFERRSGTNKKWEEFPEKVAVQMN 481

Query: 483  DTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPR 542
            DTHPTLCIPELMRILLDLKGLSWEEAW ITQRT+AYTNHTVLPEALEKWS ELMQ+LLPR
Sbjct: 482  DTHPTLCIPELMRILLDLKGLSWEEAWNITQRTMAYTNHTVLPEALEKWSFELMQKLLPR 541

Query: 543  HVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVV 602
            HVEIIE IDEELI+TIISEYG ADLKLL +KLKELRILENVDLPAAYSDL IEPEESS +
Sbjct: 542  HVEIIELIDEELIRTIISEYGKADLKLLREKLKELRILENVDLPAAYSDLFIEPEESSTI 601

Query: 603  ASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPP-KMV 662
             STE  + S E DS D+EN    AKS+ + E VE  D+PESK IQDK VEPIPPPP KMV
Sbjct: 602  VSTEVLKSSEEADSVDKENLSKLAKSVDKVEFVEANDDPESKDIQDKNVEPIPPPPPKMV 661

Query: 663  RMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDL 722
            RMANLCV GGHAVNGVAEIHSEIVKDEVFNAFY+LWPEKFQNKTNGVTPRRWIRFCNPDL
Sbjct: 662  RMANLCVVGGHAVNGVAEIHSEIVKDEVFNAFYELWPEKFQNKTNGVTPRRWIRFCNPDL 721

Query: 723  SKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVS 782
            SKLITNWIGSEDWVLNTEKLGELK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGYTVS
Sbjct: 722  SKLITNWIGSEDWVLNTEKLGELKEFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGYTVS 781

Query: 783  PDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYV 842
            PDAMFDIQVKRIHEYKRQLLNI GIVYRYKKMKEMSA+ERKE +VPRVCIFGGKAFATYV
Sbjct: 782  PDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMSAKERKETYVPRVCIFGGKAFATYV 841

Query: 843  QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS 902
            QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS
Sbjct: 842  QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS 901

Query: 903  GTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDP 962
            GTSNMKFAMNGCILIGTLDGANVEIRQEVGA+NFFLFGA+AHEIAGLRKERA GKFIPDP
Sbjct: 902  GTSNMKFAMNGCILIGTLDGANVEIRQEVGADNFFLFGAEAHEIAGLRKERAVGKFIPDP 961

Query: 963  RFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD 1022
            RFEEVKEYVRSGVFGSYDYE++IGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD
Sbjct: 962  RFEEVKEYVRSGVFGSYDYEDLIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD 975

Query: 1023 QKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            QK+WTRMSILNTAGSYKFSSDRTIHEYAKDIW+IKPVELP
Sbjct: 1022 QKRWTRMSILNTAGSYKFSSDRTIHEYAKDIWSIKPVELP 975

BLAST of MC08g1904 vs. NCBI nr
Match: TYK15871.1 (alpha-1,4 glucan phosphorylase L-1 isozyme [Cucumis melo var. makuwa])

HSP 1 Score: 1701 bits (4405), Expect = 0.0
Identity = 876/1063 (82.41%), Postives = 917/1063 (86.27%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAA+SQ T+ LN  DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS 
Sbjct: 1    MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EP  K   DPVAD+ES   A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61   EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121  SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181  ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241  VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDL+AFNAGEH++ASEALASA+K                     
Sbjct: 301  NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQM
Sbjct: 421  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQM 480

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLP
Sbjct: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLP 540

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIE IDEELI+TIISEYGTADLKLL +KLKELRILENVDLPAAYSDL IEPEESS 
Sbjct: 541  RHVEIIELIDEELIRTIISEYGTADLKLLREKLKELRILENVDLPAAYSDLFIEPEESST 600

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPP---P 660
            +ASTE  + S E DS D+EN    AK + +DE VE+ DE E K IQDKKVEP  PP   P
Sbjct: 601  IASTEVLKSSKEADSVDKENLSKLAKFVDKDEFVED-DEVECKDIQDKKVEPTSPPLPPP 660

Query: 661  KMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCN 720
            KMVRMANLCV GGHAVNGVAEIHSEIVKDEVFN+FYKLWPEKFQNKTNGVTPRRWI FCN
Sbjct: 661  KMVRMANLCVVGGHAVNGVAEIHSEIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCN 720

Query: 721  PDLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGY 780
            PDLSKLITNWIGSEDWVLNTEKLG LK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGY
Sbjct: 721  PDLSKLITNWIGSEDWVLNTEKLGGLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGY 780

Query: 781  TVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFA 840
            TVSPDAMFDIQVKRIHEYKRQLLNI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFA
Sbjct: 781  TVSPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFA 840

Query: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900
            TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM
Sbjct: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900

Query: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFI 960
            EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFI
Sbjct: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFI 960

Query: 961  PDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEA 1020
            PDPRFEEVKEYVRSGVFGSYDYEE+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEA
Sbjct: 961  PDPRFEEVKEYVRSGVFGSYDYEELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEA 976

Query: 1021 YRDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            YRDQK+WTRMSILNTAGSYKFSSDRTIHEYAKDIW+IKPVELP
Sbjct: 1021 YRDQKRWTRMSILNTAGSYKFSSDRTIHEYAKDIWSIKPVELP 976

BLAST of MC08g1904 vs. NCBI nr
Match: XP_008455590.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic-like [Cucumis melo])

HSP 1 Score: 1699 bits (4399), Expect = 0.0
Identity = 875/1063 (82.31%), Postives = 916/1063 (86.17%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAA+SQ T+ LN  DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS 
Sbjct: 1    MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EP  K   DPVAD+ES   A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61   EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121  SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181  ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241  VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDL+AFNAGEH++ASEALASA+K                     
Sbjct: 301  NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQM
Sbjct: 421  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQM 480

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLP
Sbjct: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLP 540

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIE IDEELI+TIISEYGTADLKLL +KLKELRILENVDLPAAYSDL IEPEESS 
Sbjct: 541  RHVEIIELIDEELIRTIISEYGTADLKLLREKLKELRILENVDLPAAYSDLFIEPEESST 600

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPP---P 660
            +ASTE  + S E DS D+EN    AK + +DE VE+ DE E K IQDKKVEP  PP   P
Sbjct: 601  IASTEVLKSSKEADSVDKENLSKLAKFVDKDEFVED-DEVECKDIQDKKVEPTSPPLPPP 660

Query: 661  KMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCN 720
            KMVRMANLCV GGHAVNGVAEIHSEIVKDEVFN+FYKLWPEKFQNKTNGVTPRRWI FCN
Sbjct: 661  KMVRMANLCVVGGHAVNGVAEIHSEIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCN 720

Query: 721  PDLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGY 780
            PDLSKLITNWIGSEDWVLNTEKLG LK FADNEDLQ QWR AKRNNKLKAVSFLKE TGY
Sbjct: 721  PDLSKLITNWIGSEDWVLNTEKLGGLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEXTGY 780

Query: 781  TVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFA 840
            TVSPDAMFDIQVKRIHEYKRQLLNI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFA
Sbjct: 781  TVSPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFA 840

Query: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900
            TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM
Sbjct: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900

Query: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFI 960
            EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFI
Sbjct: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFI 960

Query: 961  PDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEA 1020
            PDPRFEEVKEYVRSGVFGSYDYEE+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEA
Sbjct: 961  PDPRFEEVKEYVRSGVFGSYDYEELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEA 976

Query: 1021 YRDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            YRDQK+WTRMSILNTAGSYKFSSDRTIHEYAKDIW+IKPVELP
Sbjct: 1021 YRDQKRWTRMSILNTAGSYKFSSDRTIHEYAKDIWSIKPVELP 976

BLAST of MC08g1904 vs. NCBI nr
Match: KAA0053393.1 (alpha-1,4 glucan phosphorylase L-1 isozyme [Cucumis melo var. makuwa])

HSP 1 Score: 1697 bits (4396), Expect = 0.0
Identity = 874/1063 (82.22%), Postives = 916/1063 (86.17%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAA+SQ T+ LN  DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS 
Sbjct: 1    MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EP  K   DPVAD+ES   A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61   EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121  SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181  ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241  VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDL+AFNAGEH++ASEALASA+K                     
Sbjct: 301  NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQM
Sbjct: 421  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQM 480

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLP
Sbjct: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLP 540

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIE IDEELI+TIISEYGTADLKLL +KL ELRILENVDLPAAYSDL IEPEESS 
Sbjct: 541  RHVEIIELIDEELIRTIISEYGTADLKLLREKLNELRILENVDLPAAYSDLFIEPEESST 600

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPP---P 660
            +ASTE  + S E DS D+EN    AK + +DE VE+ DE E K IQDKKVEP  PP   P
Sbjct: 601  IASTEVLKSSKEADSVDKENLSKLAKFVDKDEFVED-DEVECKDIQDKKVEPTSPPLPPP 660

Query: 661  KMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCN 720
            KMVRMANLCV GGHAVNGVAEIHSEIVKDEVFN+FYKLWPEKFQNKTNGVTPRRWI FCN
Sbjct: 661  KMVRMANLCVVGGHAVNGVAEIHSEIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCN 720

Query: 721  PDLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGY 780
            PDLSKLITNWIGSEDWVLNTEKLG LK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGY
Sbjct: 721  PDLSKLITNWIGSEDWVLNTEKLGGLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGY 780

Query: 781  TVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFA 840
            TVSPDAMFDIQVKRIHEYKRQLLNI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFA
Sbjct: 781  TVSPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFA 840

Query: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900
            TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM
Sbjct: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900

Query: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFI 960
            EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFI
Sbjct: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFI 960

Query: 961  PDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEA 1020
            PDPRFEEVKEYVRSGVFGSYDYEE+IGSLEGNEGFGRADYFLVGKDFPSYIECQ++VDEA
Sbjct: 961  PDPRFEEVKEYVRSGVFGSYDYEELIGSLEGNEGFGRADYFLVGKDFPSYIECQKKVDEA 976

Query: 1021 YRDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            YRDQK+WTRMSILNTAGSYKFSSDRTIHEYAKDIW+IKPVELP
Sbjct: 1021 YRDQKRWTRMSILNTAGSYKFSSDRTIHEYAKDIWSIKPVELP 976

BLAST of MC08g1904 vs. ExPASy TrEMBL
Match: A0A6J1CTT4 (Alpha-1,4 glucan phosphorylase OS=Momordica charantia OX=3673 GN=LOC111014157 PE=3 SV=1)

HSP 1 Score: 1906 bits (4937), Expect = 0.0
Identity = 977/1060 (92.17%), Postives = 977/1060 (92.17%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS
Sbjct: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY
Sbjct: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE
Sbjct: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADK                     
Sbjct: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADK--------------------- 360

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM
Sbjct: 421  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP
Sbjct: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV
Sbjct: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMV 660
            VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMV
Sbjct: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMV 660

Query: 661  RMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDL 720
            RMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDL
Sbjct: 661  RMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDL 720

Query: 721  SKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVS 780
            SKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVS
Sbjct: 721  SKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVS 780

Query: 781  PDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYV 840
            PDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYV
Sbjct: 781  PDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYV 840

Query: 841  QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS 900
            QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS
Sbjct: 841  QAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEAS 900

Query: 901  GTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDP 960
            GTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDP
Sbjct: 901  GTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDP 960

Query: 961  RFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD 1020
            RFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD
Sbjct: 961  RFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRD 977

Query: 1021 QKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            QKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP
Sbjct: 1021 QKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 977

BLAST of MC08g1904 vs. ExPASy TrEMBL
Match: A0A5D3CVD4 (Alpha-1,4 glucan phosphorylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold637G00490 PE=3 SV=1)

HSP 1 Score: 1701 bits (4405), Expect = 0.0
Identity = 876/1063 (82.41%), Postives = 917/1063 (86.27%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAA+SQ T+ LN  DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS 
Sbjct: 1    MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EP  K   DPVAD+ES   A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61   EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121  SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181  ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241  VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDL+AFNAGEH++ASEALASA+K                     
Sbjct: 301  NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQM
Sbjct: 421  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQM 480

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLP
Sbjct: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLP 540

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIE IDEELI+TIISEYGTADLKLL +KLKELRILENVDLPAAYSDL IEPEESS 
Sbjct: 541  RHVEIIELIDEELIRTIISEYGTADLKLLREKLKELRILENVDLPAAYSDLFIEPEESST 600

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPP---P 660
            +ASTE  + S E DS D+EN    AK + +DE VE+ DE E K IQDKKVEP  PP   P
Sbjct: 601  IASTEVLKSSKEADSVDKENLSKLAKFVDKDEFVED-DEVECKDIQDKKVEPTSPPLPPP 660

Query: 661  KMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCN 720
            KMVRMANLCV GGHAVNGVAEIHSEIVKDEVFN+FYKLWPEKFQNKTNGVTPRRWI FCN
Sbjct: 661  KMVRMANLCVVGGHAVNGVAEIHSEIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCN 720

Query: 721  PDLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGY 780
            PDLSKLITNWIGSEDWVLNTEKLG LK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGY
Sbjct: 721  PDLSKLITNWIGSEDWVLNTEKLGGLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGY 780

Query: 781  TVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFA 840
            TVSPDAMFDIQVKRIHEYKRQLLNI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFA
Sbjct: 781  TVSPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFA 840

Query: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900
            TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM
Sbjct: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900

Query: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFI 960
            EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFI
Sbjct: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFI 960

Query: 961  PDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEA 1020
            PDPRFEEVKEYVRSGVFGSYDYEE+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEA
Sbjct: 961  PDPRFEEVKEYVRSGVFGSYDYEELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEA 976

Query: 1021 YRDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            YRDQK+WTRMSILNTAGSYKFSSDRTIHEYAKDIW+IKPVELP
Sbjct: 1021 YRDQKRWTRMSILNTAGSYKFSSDRTIHEYAKDIWSIKPVELP 976

BLAST of MC08g1904 vs. ExPASy TrEMBL
Match: A0A1S3C0V0 (Alpha-1,4 glucan phosphorylase OS=Cucumis melo OX=3656 GN=LOC103495729 PE=3 SV=1)

HSP 1 Score: 1699 bits (4399), Expect = 0.0
Identity = 875/1063 (82.31%), Postives = 916/1063 (86.17%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAA+SQ T+ LN  DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS 
Sbjct: 1    MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EP  K   DPVAD+ES   A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61   EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121  SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181  ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241  VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDL+AFNAGEH++ASEALASA+K                     
Sbjct: 301  NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQM
Sbjct: 421  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQM 480

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLP
Sbjct: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLP 540

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIE IDEELI+TIISEYGTADLKLL +KLKELRILENVDLPAAYSDL IEPEESS 
Sbjct: 541  RHVEIIELIDEELIRTIISEYGTADLKLLREKLKELRILENVDLPAAYSDLFIEPEESST 600

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPP---P 660
            +ASTE  + S E DS D+EN    AK + +DE VE+ DE E K IQDKKVEP  PP   P
Sbjct: 601  IASTEVLKSSKEADSVDKENLSKLAKFVDKDEFVED-DEVECKDIQDKKVEPTSPPLPPP 660

Query: 661  KMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCN 720
            KMVRMANLCV GGHAVNGVAEIHSEIVKDEVFN+FYKLWPEKFQNKTNGVTPRRWI FCN
Sbjct: 661  KMVRMANLCVVGGHAVNGVAEIHSEIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCN 720

Query: 721  PDLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGY 780
            PDLSKLITNWIGSEDWVLNTEKLG LK FADNEDLQ QWR AKRNNKLKAVSFLKE TGY
Sbjct: 721  PDLSKLITNWIGSEDWVLNTEKLGGLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEXTGY 780

Query: 781  TVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFA 840
            TVSPDAMFDIQVKRIHEYKRQLLNI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFA
Sbjct: 781  TVSPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFA 840

Query: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900
            TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM
Sbjct: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900

Query: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFI 960
            EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFI
Sbjct: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFI 960

Query: 961  PDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEA 1020
            PDPRFEEVKEYVRSGVFGSYDYEE+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEA
Sbjct: 961  PDPRFEEVKEYVRSGVFGSYDYEELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEA 976

Query: 1021 YRDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            YRDQK+WTRMSILNTAGSYKFSSDRTIHEYAKDIW+IKPVELP
Sbjct: 1021 YRDQKRWTRMSILNTAGSYKFSSDRTIHEYAKDIWSIKPVELP 976

BLAST of MC08g1904 vs. ExPASy TrEMBL
Match: A0A5A7UBY0 (Alpha-1,4 glucan phosphorylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold428G00580 PE=3 SV=1)

HSP 1 Score: 1697 bits (4396), Expect = 0.0
Identity = 874/1063 (82.22%), Postives = 916/1063 (86.17%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAA+SQ T+ LN  DSFSH+NSFP+L+GLS +Y +SKLLL+ TS+WRS +RTF VKNVS 
Sbjct: 1    MAATSQFTLALNPPDSFSHSNSFPSLIGLSSRYRQSKLLLLPTSSWRSPKRTFLVKNVSG 60

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EP  K   DPVAD+ES   A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 61   EPMLK---DPVADQESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 121  SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 180

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 181  ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 240

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWE+VRNDIKY IKFYGKVV GSDGKKNWTGGEDIEAVAYDVPIPGYKTK
Sbjct: 241  VAENWLEIGNPWEIVRNDIKYHIKFYGKVVTGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDL+AFNAGEH++ASEALASA+K                     
Sbjct: 301  NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 360

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQM
Sbjct: 421  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQM 480

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAW ITQRTVAYTNHTVLPEALEKW+ ELMQ+LLP
Sbjct: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWNITQRTVAYTNHTVLPEALEKWNYELMQRLLP 540

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIE IDEELI+TIISEYGTADLKLL +KL ELRILENVDLPAAYSDL IEPEESS 
Sbjct: 541  RHVEIIELIDEELIRTIISEYGTADLKLLREKLNELRILENVDLPAAYSDLFIEPEESST 600

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPP---P 660
            +ASTE  + S E DS D+EN    AK + +DE VE+ DE E K IQDKKVEP  PP   P
Sbjct: 601  IASTEVLKSSKEADSVDKENLSKLAKFVDKDEFVED-DEVECKDIQDKKVEPTSPPLPPP 660

Query: 661  KMVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCN 720
            KMVRMANLCV GGHAVNGVAEIHSEIVKDEVFN+FYKLWPEKFQNKTNGVTPRRWI FCN
Sbjct: 661  KMVRMANLCVVGGHAVNGVAEIHSEIVKDEVFNSFYKLWPEKFQNKTNGVTPRRWILFCN 720

Query: 721  PDLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGY 780
            PDLSKLITNWIGSEDWVLNTEKLG LK FADNEDLQ QWR AKRNNKLKAVSFLKEKTGY
Sbjct: 721  PDLSKLITNWIGSEDWVLNTEKLGGLKKFADNEDLQNQWRIAKRNNKLKAVSFLKEKTGY 780

Query: 781  TVSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFA 840
            TVSPDAMFDIQVKRIHEYKRQLLNI GIVYRYKKMKEMSA+ERKE ++PRVCIFGGKAFA
Sbjct: 781  TVSPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMSAKERKETYIPRVCIFGGKAFA 840

Query: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900
            TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM
Sbjct: 841  TYVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGM 900

Query: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFI 960
            EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFI
Sbjct: 901  EASGTSNMKFAMNGCILIGTLDGANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFI 960

Query: 961  PDPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEA 1020
            PDPRFEEVKEYVRSGVFGSYDYEE+IGSLEGNEGFGRADYFLVGKDFPSYIECQ++VDEA
Sbjct: 961  PDPRFEEVKEYVRSGVFGSYDYEELIGSLEGNEGFGRADYFLVGKDFPSYIECQKKVDEA 976

Query: 1021 YRDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            YRDQK+WTRMSILNTAGSYKFSSDRTIHEYAKDIW+IKPVELP
Sbjct: 1021 YRDQKRWTRMSILNTAGSYKFSSDRTIHEYAKDIWSIKPVELP 976

BLAST of MC08g1904 vs. ExPASy TrEMBL
Match: A0A0A0KXS1 (Alpha-1,4 glucan phosphorylase OS=Cucumis sativus OX=3659 GN=Csa_4G095050 PE=3 SV=1)

HSP 1 Score: 1687 bits (4368), Expect = 0.0
Identity = 872/1062 (82.11%), Postives = 911/1062 (85.78%), Query Frame = 0

Query: 1    MAASSQLTVNLNRADSFSHANSFPTLLGLSHKYGKSKLLLVRTSNWRSARRTFAVKNVSS 60
            MAA+SQ T+ LN   SFSH+ SFP+L+GLS +Y +SK LL+ TS+WRS +RTF V+NVSS
Sbjct: 57   MAATSQFTLALNPPHSFSHSYSFPSLIGLSSRYRQSKFLLLSTSSWRSPKRTFLVRNVSS 116

Query: 61   EPKQKLKDDPVADEESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 120
            EPK K   DPVADEES   A+AF PDA+SIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ
Sbjct: 117  EPKLK---DPVADEESPTAATAFAPDASSIASSIKYHAEFTPLFSPDRFDLPKAFFATAQ 176

Query: 121  SVRDALIINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGY 180
            SVRDALIINWN T+ELYE+LNVKQAYYLSMEFLQGRALLNAIGNLELTG YAEALSKLGY
Sbjct: 177  SVRDALIINWNETFELYERLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSKLGY 236

Query: 181  ELENVACQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEE 240
            ELENVA QEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRY+YGLFKQKITKDGQEE
Sbjct: 237  ELENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQKITKDGQEE 296

Query: 241  VAENWLEIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTK 300
            VAENWLEIGNPWE+VRNDIKY IKFYGKVVIGSDGKKNWTGGEDIEAVA+DVPIPGYKTK
Sbjct: 297  VAENWLEIGNPWEIVRNDIKYHIKFYGKVVIGSDGKKNWTGGEDIEAVAHDVPIPGYKTK 356

Query: 301  NTINLRLWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCI 360
            NTINLRLWSTKAPTEDFDL+AFNAGEH++ASEALASA+K                     
Sbjct: 357  NTINLRLWSTKAPTEDFDLAAFNAGEHSRASEALASAEK--------------------- 416

Query: 361  GLKWLKLIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYL 420
                                                                        
Sbjct: 417  ------------------------------------------------------------ 476

Query: 421  LQICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQM 480
              ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIV RF RRSGAN KWEEFPEKVAVQM
Sbjct: 477  --ICHVLYPGDDSIEGKILRLKQQYTLCSASLQDIVERFVRRSGANIKWEEFPEKVAVQM 536

Query: 481  NDTHPTLCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLP 540
            NDTHPTLCIPELMRILLDLKGLSWEEAW +TQRTVAYTNHTVLPEALEKW+ ELMQ+LLP
Sbjct: 537  NDTHPTLCIPELMRILLDLKGLSWEEAWNVTQRTVAYTNHTVLPEALEKWNFELMQRLLP 596

Query: 541  RHVEIIEQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSV 600
            RHVEIIE IDEELI+TIISEYGTADLKLL +KLKELRILENVDLPAAYSDL IEPEESS 
Sbjct: 597  RHVEIIELIDEELIRTIISEYGTADLKLLREKLKELRILENVDLPAAYSDLFIEPEESST 656

Query: 601  VASTEEPEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPP--K 660
            +ASTE  + S E DS D+            DE VE +DE ESKGIQDKKVEP PPPP  K
Sbjct: 657  IASTEVLKRSKEADSVDK------------DEFVEVDDELESKGIQDKKVEPTPPPPPPK 716

Query: 661  MVRMANLCVAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNP 720
            MVRMANL V GGHAVNGVAEIHSEIVKDEVFNAFYKLWP KFQNKTNGVTPRRWI FCNP
Sbjct: 717  MVRMANLSVVGGHAVNGVAEIHSEIVKDEVFNAFYKLWPGKFQNKTNGVTPRRWILFCNP 776

Query: 721  DLSKLITNWIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYT 780
            DLSKLITNWIGSEDWVLNTEKLG LK FAD+EDLQ QWR AKRNNKLKAVSFLKEKTGYT
Sbjct: 777  DLSKLITNWIGSEDWVLNTEKLGGLKKFADDEDLQNQWRIAKRNNKLKAVSFLKEKTGYT 836

Query: 781  VSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFAT 840
            VSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSA+ERKE +VPRVCIFGGKAFAT
Sbjct: 837  VSPDAMFDIQVKRIHEYKRQLLNILGIVYRYKKMKEMSAKERKETYVPRVCIFGGKAFAT 896

Query: 841  YVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGME 900
            YVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGME
Sbjct: 897  YVQAKRIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGME 956

Query: 901  ASGTSNMKFAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIP 960
            ASGTSNMKFAMNGCILIGTLDGANVEIRQEVGA+NFFLFGA+AHEIAGLRKERAEGKFIP
Sbjct: 957  ASGTSNMKFAMNGCILIGTLDGANVEIRQEVGADNFFLFGAEAHEIAGLRKERAEGKFIP 1016

Query: 961  DPRFEEVKEYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAY 1020
            DPRFEEVKEYVRSGVFGS DYEE+I SLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAY
Sbjct: 1017 DPRFEEVKEYVRSGVFGSCDYEELIASLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAY 1020

Query: 1021 RDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1060
            RDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIW+IKPVELP
Sbjct: 1077 RDQKKWTRMSILNTAGSYKFSSDRTIHEYAKDIWSIKPVELP 1020

BLAST of MC08g1904 vs. TAIR 10
Match: AT3G29320.1 (Glycosyl transferase, family 35 )

HSP 1 Score: 1449.1 bits (3750), Expect = 0.0e+00
Identity = 742/1054 (70.40%), Postives = 838/1054 (79.51%), Query Frame = 0

Query: 14   ADSFSHANSFPTLLGLSHKYGK--SKLLLVRTSNWRSA--RRTF-AVKNVSSEPKQKLKD 73
            A+     NS  +L+      GK  +++   R  + R +  RR+F +VK++SSEPK K+ D
Sbjct: 13   AEVLIQCNSLSSLVSRRCDDGKWRTRMFPARNRDLRPSPTRRSFLSVKSISSEPKAKVTD 72

Query: 74   DPVADEESSIVAS--AFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDAL 133
              +  E+   ++S   F PDAAS+ASSIKYHAEFTPLFSP++F+LPKAFFATAQSVRDAL
Sbjct: 73   AVLDSEQEVFISSMNPFAPDAASVASSIKYHAEFTPLFSPEKFELPKAFFATAQSVRDAL 132

Query: 134  IINWNATYELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVA 193
            I+NWNATYE Y ++NVKQAYYLSMEFLQGRAL NA+GNL L  AY +AL +LG++LE+VA
Sbjct: 133  IMNWNATYEYYNRVNVKQAYYLSMEFLQGRALSNAVGNLGLNSAYGDALKRLGFDLESVA 192

Query: 194  CQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWL 253
             QEPD ALGNGGLGRLASCFLDS+ATLNYPAWGYGLRY+YGLFKQ+ITKDGQEE AE+WL
Sbjct: 193  SQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEAAEDWL 252

Query: 254  EIGNPWEVVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLR 313
            E+ NPWE+VRND+ YPIKFYGKVV GSDGKK W GGEDI AVAYDVPIPGYKTK TINLR
Sbjct: 253  ELSNPWEIVRNDVSYPIKFYGKVVFGSDGKKRWIGGEDIVAVAYDVPIPGYKTKTTINLR 312

Query: 314  LWSTKAPTEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLK 373
            LWSTKAP+EDFDLS++N+G+HT+A+EAL +A+K                           
Sbjct: 313  LWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEK--------------------------- 372

Query: 374  LIIEFLVRKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYLLQICHV 433
                                                                    IC V
Sbjct: 373  --------------------------------------------------------ICFV 432

Query: 434  LYPGDDSIEGKILRLKQQYTLCSASLQDIVARFERRSGANKKWEEFPEKVAVQMNDTHPT 493
            LYPGD+S EGK LRLKQQYTLCSASLQDIVARFE RSG N  WEEFPEKVAVQMNDTHPT
Sbjct: 433  LYPGDESTEGKALRLKQQYTLCSASLQDIVARFETRSGGNVNWEEFPEKVAVQMNDTHPT 492

Query: 494  LCIPELMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEII 553
            LCIPELMRIL+DLKGLSWE+AWKITQRTVAYTNHTVLPEALEKWSLELM++LLPRHVEII
Sbjct: 493  LCIPELMRILMDLKGLSWEDAWKITQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEII 552

Query: 554  EQIDEELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEE 613
            E+IDEEL++TI+SEYGTAD  LL +KLK +RILENV+LP+A++D+I++P    V A    
Sbjct: 553  EKIDEELVRTIVSEYGTADPDLLEEKLKAMRILENVELPSAFADVIVKPVNKPVTAK--- 612

Query: 614  PEISNEVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMVRMANLC 673
             +  N V +  EE      K+ GE    EEEDE           EP   PPKMVRMANL 
Sbjct: 613  -DAQNGVKTEQEEE-----KTAGE----EEEDEVIP--------EPTVEPPKMVRMANLA 672

Query: 674  VAGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKLITN 733
            V GGHAVNGVAEIHSEIVK +VFN F +LWPEKFQNKTNGVTPRRWIRFCNP LS +ITN
Sbjct: 673  VVGGHAVNGVAEIHSEIVKQDVFNDFVQLWPEKFQNKTNGVTPRRWIRFCNPYLSDIITN 732

Query: 734  WIGSEDWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFD 793
            WIG+EDWVLNTEK+ EL+ FADNEDLQ++WR AK+ NKLK VS +KE+TGYTVSPDAMFD
Sbjct: 733  WIGTEDWVLNTEKVAELRKFADNEDLQSEWRAAKKKNKLKVVSLIKERTGYTVSPDAMFD 792

Query: 794  IQVKRIHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIV 853
            IQ+KRIHEYKRQLLNILGIVYRYKKMKEMSA ER++AFVPRVCIFGGKAFATYVQAKRIV
Sbjct: 793  IQIKRIHEYKRQLLNILGIVYRYKKMKEMSASEREKAFVPRVCIFGGKAFATYVQAKRIV 852

Query: 854  KFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMK 913
            KFITDV +T+NHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMK
Sbjct: 853  KFITDVASTINHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMK 912

Query: 914  FAMNGCILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVK 973
            F+MNGC+LIGTLDGANVEIR+EVG ENFFLFGAKA +I  LRKERAEGKF+PDP FEEVK
Sbjct: 913  FSMNGCVLIGTLDGANVEIREEVGEENFFLFGAKADQIVNLRKERAEGKFVPDPTFEEVK 962

Query: 974  EYVRSGVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTR 1033
            ++V SGVFGS  Y+E+IGSLEGNEGFGRADYFLVGKDFPSYIECQE+VDEAYRDQK+WTR
Sbjct: 973  KFVGSGVFGSNSYDELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTR 962

Query: 1034 MSILNTAGSYKFSSDRTIHEYAKDIWNIKPVELP 1061
            MSI+NTAGS+KFSSDRTIHEYAKDIWNIK VELP
Sbjct: 1033 MSIMNTAGSFKFSSDRTIHEYAKDIWNIKQVELP 962

BLAST of MC08g1904 vs. TAIR 10
Match: AT3G46970.1 (alpha-glucan phosphorylase 2 )

HSP 1 Score: 1025.0 bits (2649), Expect = 4.3e-299
Identity = 526/989 (53.19%), Postives = 653/989 (66.03%), Query Frame = 0

Query: 74   EESSIVASAFTPDAASIASSIKYHAEFTPLFSPDRFDLPKAFFATAQSVRDALIINWNAT 133
            E+ S  A+    DA  IA +I YHA+++P FSP +F   +A +ATA+S+RD LI  WN T
Sbjct: 14   EKISAKANPEADDATEIAGNIVYHAKYSPHFSPLKFGPEQALYATAESLRDRLIQLWNET 73

Query: 134  YELYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGAYAEALSKLGYELENVACQEPDAA 193
            Y  + K++ KQ YYLSME+LQGRAL NAIGNL L G YA+AL  LGYELE +A QE DAA
Sbjct: 74   YVHFNKVDPKQTYYLSMEYLQGRALTNAIGNLNLQGPYADALRTLGYELEEIAEQEKDAA 133

Query: 194  LGNGGLGRLASCFLDSLATLNYPAWGYGLRYRYGLFKQKITKDGQEEVAENWLEIGNPWE 253
            LGNGGLGRLASCFLDS+ATLN PAWGYGLRYR+GLFKQ ITK GQEE+ E+WLE  +PWE
Sbjct: 134  LGNGGLGRLASCFLDSMATLNLPAWGYGLRYRHGLFKQIITKKGQEEIPEDWLEKFSPWE 193

Query: 254  VVRNDIKYPIKFYGKVVIGSDGKKNWTGGEDIEAVAYDVPIPGYKTKNTINLRLWSTKAP 313
            +VR+D+ +P++F+GKV +  DG + W  G+ ++A+AYDVPIPGY TKNTI+LRLW  KA 
Sbjct: 194  IVRHDVVFPVRFFGKVQVNPDGSRKWVDGDVVQALAYDVPIPGYGTKNTISLRLWEAKAR 253

Query: 314  TEDFDLSAFNAGEHTKASEALASADKVQLLDCTNSLLWFQLSLLKCIGLKWLKLIIEFLV 373
             ED DL  FN GE+  A++  + A                                    
Sbjct: 254  AEDLDLFQFNEGEYELAAQLHSRAQ----------------------------------- 313

Query: 374  RKIENFMKRNHLTFQYFNEKFCFLKKKKGPESSVCTLKFYKNGSSYLLQICHVLYPGDDS 433
                                                            QIC VLYPGD +
Sbjct: 314  ------------------------------------------------QICTVLYPGDAT 373

Query: 434  IEGKILRLKQQYTLCSASLQDIVARFERRS--GANKKWEEFPEKVAVQMNDTHPTLCIPE 493
              GK+LRLKQQ+ LCSASLQDI++RF  RS    ++KW EFP KVAVQMNDTHPTL IPE
Sbjct: 374  ENGKLLRLKQQFFLCSASLQDIISRFHERSTTEGSRKWSEFPSKVAVQMNDTHPTLAIPE 433

Query: 494  LMRILLDLKGLSWEEAWKITQRTVAYTNHTVLPEALEKWSLELMQQLLPRHVEIIEQIDE 553
            LMR+L+D  GL W+EAW +T +TVAYTNHTVLPEALEKWS  LM +LLPRH+EIIE+ID+
Sbjct: 434  LMRLLMDDNGLGWDEAWDVTSKTVAYTNHTVLPEALEKWSQSLMWKLLPRHMEIIEEIDK 493

Query: 554  ELIQTIISEYGTADLKLLHKKLKELRILENVDLPAAYSDLIIEPEESSVVASTEEPEISN 613
              +QTI                                                      
Sbjct: 494  RFVQTI------------------------------------------------------ 553

Query: 614  EVDSADEENSEGQAKSIGEDETVEEEDEPESKGIQDKKVEPIPPPPKMVRMANLCVAGGH 673
                               D  V+ ED+  S  I D       P   +VRMANLCV   H
Sbjct: 554  ------------------RDTRVDLEDKISSLSILDNN-----PQKPVVRMANLCVVSSH 613

Query: 674  AVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKLITNWIGSE 733
             VNGVA++HS+I+K E+F  +  +WP KFQNKTNG+TPRRW+RFC+P+LS +IT W+ ++
Sbjct: 614  TVNGVAQLHSDILKAELFADYVSIWPNKFQNKTNGITPRRWLRFCSPELSDIITKWLKTD 673

Query: 734  DWVLNTEKLGELKMFADNEDLQTQWRTAKRNNKLKAVSFLKEKTGYTVSPDAMFDIQVKR 793
             W+ + + L  L+ FADNE+LQ++W +AK  NK +   +++  TG ++ P ++FDIQVKR
Sbjct: 674  KWITDLDLLTGLRQFADNEELQSEWASAKTANKKRLAQYIERVTGVSIDPTSLFDIQVKR 733

Query: 794  IHEYKRQLLNILGIVYRYKKMKEMSAEERKEAFVPRVCIFGGKAFATYVQAKRIVKFITD 853
            IHEYKRQL+NILG+VYR+KK+KEM  EERK+  VPR  + GGKAFATY  AKRIVK + D
Sbjct: 734  IHEYKRQLMNILGVVYRFKKLKEMKPEERKKT-VPRTVMIGGKAFATYTNAKRIVKLVND 793

Query: 854  VGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNG 913
            VG  VN DPE+ + LKV+FVP+YNV+VAE+LIP SELSQHISTAGMEASGTSNMKFA+NG
Sbjct: 794  VGDVVNSDPEVNEYLKVVFVPNYNVTVAEMLIPGSELSQHISTAGMEASGTSNMKFALNG 841

Query: 914  CILIGTLDGANVEIRQEVGAENFFLFGAKAHEIAGLRKERAEGKFIPDPRFEEVKEYVRS 973
            C++IGTLDGANVEIR+EVG ENFFLFGA A ++  LRKER +G F PDPRFEE K++V+S
Sbjct: 854  CLIIGTLDGANVEIREEVGEENFFLFGATADQVPRLRKEREDGLFKPDPRFEEAKQFVKS 841

Query: 974  GVFGSYDYEEMIGSLEGNEGFGRADYFLVGKDFPSYIECQEQVDEAYRDQKKWTRMSILN 1033
            GVFGSYDY  ++ SLEGN GFGR DYFLVG DFPSY++ Q +VDEAY+D+K W +MSIL+
Sbjct: 914  GVFGSYDYGPLLDSLEGNTGFGRGDYFLVGYDFPSYMDAQAKVDEAYKDRKGWLKMSILS 841

Query: 1034 TAGSYKFSSDRTIHEYAKDIWNIKPVELP 1061
            TAGS KFSSDRTI +YAK+IWNI+   +P
Sbjct: 974  TAGSGKFSSDRTIAQYAKEIWNIEACPVP 841