MC08g1877 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1877
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAbhydrolase_3 domain-containing protein
LocationMC08: 27009536 .. 27010561 (-)
RNA-Seq ExpressionMC08g1877
SyntenyMC08g1877
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCATTTCCATCGATCCAAGGCTCAAACTCCAGATGCCCAAATCCCATCTCCAGTGTGCATCTACGGCAGAGGAGATTGAAGGGCTTATTCGAGTTTATGCAGATGGGCATGTCGAAAGGCCCCCAATCATCCCAAATGTACCCTGCAGCACCACAGCATCAAACAAAGGAGTGTCTGCAAAAGATGTTCTCATAGACAATTTCACAAACACTTGGGCGCGCATATACATTGCCAACTCTCAACGAAAGCTCCCCCTTCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCCGCTGCCTGGAGCTGTTACCATGAGTTCCTCCTCGATCTAGCTTCCAAAGCAAAATGCGCAATCCTCTCCATCAACTACCGTCTTTCACCTGAAAATCGTCTCCCCGCCGCTTACGAGGACGGATTCAACGCGCTCATGTGGTTGAAACAGCAAGCAATCAGCGAGCCAAATGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAACTTGTTCCTCGCCGGCGACAGCGCCGGCGCTAACATAGCATACAACATCGCCACACGGCCAGGGCTCAACGAACCCGCAACCCTAAAACCTCTAACCCTAAAGGGCGTAATCCTGATCCAACCTTTCTTCGGCGGAGAATCGCGAACGAAATCAGAAAAGCTCGCCGCTCAGCCCTCCACCTCCGCGCTAACTCTTGCCACCGCCGACACATATTGGCGCCTGTCCCTGCCGCCGGGGGCCAGCCGCGACCATCCATGGAGCAACCCGCTGGTAAATGGCGGGACAAAGCTGAGAGATACGAAGCTTCCGCCGTCGATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAATTCTGTGCGGCCATGGCTGCCGCCGGGAAGAAAGTGGAGAGAGTGATTTTCAAAGGAGTTGGACATGGATTCCAAATTTTGAACAAAGACCAAATCTCCGCCATACGAACCCAAGAAATGATGGTTTCTATTCAGAATTTCATTCACCAA

mRNA sequence

ATGGCTGCCATTTCCATCGATCCAAGGCTCAAACTCCAGATGCCCAAATCCCATCTCCAGTGTGCATCTACGGCAGAGGAGATTGAAGGGCTTATTCGAGTTTATGCAGATGGGCATGTCGAAAGGCCCCCAATCATCCCAAATGTACCCTGCAGCACCACAGCATCAAACAAAGGAGTGTCTGCAAAAGATGTTCTCATAGACAATTTCACAAACACTTGGGCGCGCATATACATTGCCAACTCTCAACGAAAGCTCCCCCTTCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCCGCTGCCTGGAGCTGTTACCATGAGTTCCTCCTCGATCTAGCTTCCAAAGCAAAATGCGCAATCCTCTCCATCAACTACCGTCTTTCACCTGAAAATCGTCTCCCCGCCGCTTACGAGGACGGATTCAACGCGCTCATGTGGTTGAAACAGCAAGCAATCAGCGAGCCAAATGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAACTTGTTCCTCGCCGGCGACAGCGCCGGCGCTAACATAGCATACAACATCGCCACACGGCCAGGGCTCAACGAACCCGCAACCCTAAAACCTCTAACCCTAAAGGGCGTAATCCTGATCCAACCTTTCTTCGGCGGAGAATCGCGAACGAAATCAGAAAAGCTCGCCGCTCAGCCCTCCACCTCCGCGCTAACTCTTGCCACCGCCGACACATATTGGCGCCTGTCCCTGCCGCCGGGGGCCAGCCGCGACCATCCATGGAGCAACCCGCTGGTAAATGGCGGGACAAAGCTGAGAGATACGAAGCTTCCGCCGTCGATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAATTCTGTGCGGCCATGGCTGCCGCCGGGAAGAAAGTGGAGAGAGTGATTTTCAAAGGAGTTGGACATGGATTCCAAATTTTGAACAAAGACCAAATCTCCGCCATACGAACCCAAGAAATGATGGTTTCTATTCAGAATTTCATTCACCAA

Coding sequence (CDS)

ATGGCTGCCATTTCCATCGATCCAAGGCTCAAACTCCAGATGCCCAAATCCCATCTCCAGTGTGCATCTACGGCAGAGGAGATTGAAGGGCTTATTCGAGTTTATGCAGATGGGCATGTCGAAAGGCCCCCAATCATCCCAAATGTACCCTGCAGCACCACAGCATCAAACAAAGGAGTGTCTGCAAAAGATGTTCTCATAGACAATTTCACAAACACTTGGGCGCGCATATACATTGCCAACTCTCAACGAAAGCTCCCCCTTCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCCGCTGCCTGGAGCTGTTACCATGAGTTCCTCCTCGATCTAGCTTCCAAAGCAAAATGCGCAATCCTCTCCATCAACTACCGTCTTTCACCTGAAAATCGTCTCCCCGCCGCTTACGAGGACGGATTCAACGCGCTCATGTGGTTGAAACAGCAAGCAATCAGCGAGCCAAATGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAACTTGTTCCTCGCCGGCGACAGCGCCGGCGCTAACATAGCATACAACATCGCCACACGGCCAGGGCTCAACGAACCCGCAACCCTAAAACCTCTAACCCTAAAGGGCGTAATCCTGATCCAACCTTTCTTCGGCGGAGAATCGCGAACGAAATCAGAAAAGCTCGCCGCTCAGCCCTCCACCTCCGCGCTAACTCTTGCCACCGCCGACACATATTGGCGCCTGTCCCTGCCGCCGGGGGCCAGCCGCGACCATCCATGGAGCAACCCGCTGGTAAATGGCGGGACAAAGCTGAGAGATACGAAGCTTCCGCCGTCGATGGTGTGCATATCGGAGTTGGACATACTGGTGGACAGAAACTTGGAATTCTGTGCGGCCATGGCTGCCGCCGGGAAGAAAGTGGAGAGAGTGATTTTCAAAGGAGTTGGACATGGATTCCAAATTTTGAACAAAGACCAAATCTCCGCCATACGAACCCAAGAAATGATGGTTTCTATTCAGAATTTCATTCACCAA

Protein sequence

MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGVSAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAGANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAAGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ
Homology
BLAST of MC08g1877 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.8e-95
Identity = 179/329 (54.41%), Postives = 223/329 (67.78%), Query Frame = 0

Query: 18  HLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGVSAKDVLIDNFTNTWARI 77
           H +     EEIEGLI+V+ DG VERPPI+P V  +   S+K  +A D+ + N  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFDIKLSN--DTWTRV 81

Query: 78  YI-----ANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPE 137
           YI     A+    LPLLVYFHGGGFCVGSAAWSCYH+FL  LA KA+C I+S+NYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 138 NRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAGANIAYNIATRPG 197
           +RLPAAY+DG N + WL +Q IS    +  WL  CN SN+FLAGDSAGANIAY +A R  
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI- 201

Query: 198 LNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGAS 257
           +        L LKG+ILI PFFGGESRT SEK      +SALTL+ +D YWRL+LP GAS
Sbjct: 202 MASGKYANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGAS 261

Query: 258 RDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAAGKKVERVIFKGV 317
           RDHPW NPL++        KLP +MV ++E DIL +RNLE C  M + GK+VE ++  GV
Sbjct: 262 RDHPWCNPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGV 321

Query: 318 GHGFQILNKDQISAIRTQEMMVSIQNFIH 342
           GH F IL+   +S  R  +MM  + NFIH
Sbjct: 322 GHAFHILDNSSVSRDRIHDMMCRLHNFIH 342

BLAST of MC08g1877 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.7e-82
Identity = 151/321 (47.04%), Postives = 211/321 (65.73%), Query Frame = 0

Query: 26  EEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGVSAKDVLIDNFTNTWARIYI-----A 85
           +E+EGLI+VY DGHVER  ++P V  S      GV+  DV+ID  TN WAR+Y+      
Sbjct: 25  DEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVPMTTTK 84

Query: 86  NSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPENRLPAAYE 145
           +S  KLPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C ++S+NYRL+PEN LPAAYE
Sbjct: 85  SSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYE 144

Query: 146 DGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAGANIAYNIATRPGLNEPATLK 205
           DG NA++WL     ++      W   C+   +FLAGDSAG NIA  +A R    E   LK
Sbjct: 145 DGVNAILWL-----NKARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPEDLALK 204

Query: 206 PLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGASRDHPWSNP 265
              ++G ILIQPF+ GE RT+SE+      T+ LTLA++D +WR+SLP GA+R+HP+  P
Sbjct: 205 ---IEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKP 264

Query: 266 LVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAAGKKVERVIFKGVGHGFQILN 325
           +      ++ + +  ++VC++E+D+L+D N+E C         ++RV+ KGVGH F IL 
Sbjct: 265 V---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDV---IKRVLHKGVGHAFHILG 324

Query: 326 KDQISAIRTQEMMVSIQNFIH 342
           K Q++   T EM+  I  FIH
Sbjct: 325 KSQLAHTTTLEMLCQIDAFIH 330

BLAST of MC08g1877 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.9e-49
Identity = 114/298 (38.26%), Postives = 170/298 (57.05%), Query Frame = 0

Query: 25  AEEIEGLIRVYADGHVERPPIIPNVPCSTT-ASNKGVSAKDVLIDNFTNTWARIY---IA 84
           AE+  GL+++ ++G V R   I  +       +N+ V  KD +     N   R+Y    A
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  NSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPENRLPAAYE 144
           +++  LP++V+FHGGGFC GS +W  +H F L LAS     ++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAISEPNEHKWWLG-NCNPSNLFLAGDSAGANIAYNIATRPGLNEPATL 204
           D    L WL  QA+S+   H +  G + +   +F+ GDS+G NIA+ +A R G +    L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGASRDHPWSN 264
            P+ ++G +L+ PFFGGE RT SE     PS + L+L   D +WRLSLP GA+RDH  +N
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 250

Query: 265 PLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAM-AAAGKKVERVIFKGVGHGF 317
           P       L    L P +V +   ++L DR  E+   +    GK+V+ + F+   HGF
Sbjct: 251 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of MC08g1877 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.6e-42
Identity = 109/320 (34.06%), Postives = 170/320 (53.12%), Query Frame = 0

Query: 37  DGHVERPPIIPNVPCSTTAS-----NKGVSAKDVLIDNFTNTWARIYIAN--------SQ 96
           DG + R   + N PC+         N  VS KD+ ++   +TW R+Y+ +        S 
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVS-KDLPVNQLKSTWLRLYLPSSAVNEGNVSS 80

Query: 97  RKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPENRLPAAYEDGF 156
           +KLP++VY+HGGGF + S     +H+F  ++A      ++S +YRL+PE+RLPAAY+DG 
Sbjct: 81  QKLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGV 140

Query: 157 NALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAGANIAYNIATRPGLNEPATLKPLT 216
            AL W+K       ++ +W   + + SN+FL G SAG N+AYN+  R  ++  + L PL 
Sbjct: 141 EALDWIK------TSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLR-SVDSVSDLSPLQ 200

Query: 217 LKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGASRDHPWSNPLVN 276
           ++G+IL  PFFGGE R++SE      +         D  W LSLP G  RDH +SNP V 
Sbjct: 201 IRGLILHHPFFGGEERSESE--IRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 277 GGTKLRDT--KLPPSMVCI-SELDILVDRNLEFCAAMAAAGKKVERVIFKGVGHGFQILN 336
            G++  +   +L   ++ I  E D ++D   +    M   G +V      G  HG +I +
Sbjct: 261 DGSEKLEKIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIRD 320

Query: 337 KDQISAIRTQEMMVSIQNFI 341
                  + + + +SI+NFI
Sbjct: 321 PS-----KRKTLFLSIKNFI 323

BLAST of MC08g1877 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 8.8e-41
Identity = 110/341 (32.26%), Postives = 168/341 (49.27%), Query Frame = 0

Query: 14  MPKSHLQCASTAEEIEGLIR-----VYADGHVERPPIIPNVPCS-TTASNKGVSAKDVLI 73
           M   HL+   +++    L++     +  D  + RP  IP+   S    S+  V  KD+ +
Sbjct: 1   MSNDHLETTGSSDPNTNLLKYLPIVLNPDRTITRPIQIPSTAASPDPTSSSPVLTKDLAL 60

Query: 74  DNFTNTWARIYIAN----SQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAIL 133
           +   NT+ R+++      +  KLPL+VYFHGGGF + SAA + +H+F  ++A  A   I 
Sbjct: 61  NPLHNTFVRLFLPRHALYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIA 120

Query: 134 SINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAGANI 193
           S++YRL+PE+RLPAAY+D   AL W+K       +  +W     + SN F+ G+SAG NI
Sbjct: 121 SVDYRLAPEHRLPAAYDDAMEALQWIKD------SRDEWLTNFADFSNCFIMGESAGGNI 180

Query: 194 AYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYW 253
           AY+   R        L PL +KG++L +P FGG  RT SE   A  + S L     D  W
Sbjct: 181 AYHAGLRAAAVADELL-PLKIKGLVLDEPGFGGSKRTGSELRLA--NDSRLPTFVLDLIW 240

Query: 254 RLSLPPGASRDHPWSNPLVNGGTKLRDTKLPP----SMVCISELDILVDRNLEFCAAMAA 313
            LSLP GA RDH + NP           K+       MV     D ++DR +E    +  
Sbjct: 241 ELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLEK 300

Query: 314 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFI 341
            G  V      G  H  ++ + +     + ++  V ++ F+
Sbjct: 301 KGVDVVAQFDVGGYHAVKLEDPE-----KAKQFFVILKKFV 327

BLAST of MC08g1877 vs. NCBI nr
Match: XP_022155020.1 (probable carboxylesterase 17 [Momordica charantia])

HSP 1 Score: 698 bits (1801), Expect = 1.58e-253
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
           AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATAD 240
           ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATAD
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATAD 245

Query: 241 TYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAA 300
           TYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAA
Sbjct: 246 TYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAA 305

Query: 301 GKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
           GKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ
Sbjct: 306 GKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of MC08g1877 vs. NCBI nr
Match: XP_022998605.1 (probable carboxylesterase 17 [Cucurbita maxima])

HSP 1 Score: 597 bits (1538), Expect = 1.41e-213
Identity = 295/343 (86.01%), Postives = 311/343 (90.67%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAA+ IDPRLKLQM K HLQ  +T+EEIEGLIRVYADGHVERPPIIPNV CSTTASNKGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
            ILSINYRLSPENRLPAAYEDGFNA+MWLKQQAI+ P EHKWWLGNCNPS LFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPS-TSALTLATA 240
           ANIAYN ATR G NE   LKP TLKG+ILIQPFFGGESRTKSEKLAA PS TSALTL+ +
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 300
           DTYWRLSLPP A+RDHPWSNPLVNGG KL++ KLPP MVCISELDIL+DRNLEFCAAMAA
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
           AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 343

BLAST of MC08g1877 vs. NCBI nr
Match: XP_022948283.1 (probable carboxylesterase 17 [Cucurbita moschata])

HSP 1 Score: 595 bits (1535), Expect = 4.87e-213
Identity = 294/343 (85.71%), Postives = 310/343 (90.38%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAA+ IDPRLKLQM K HLQ   T+EEIEGLIRVYADGHVERPPIIPNV CSTTAS+KGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
            ILSINYRLSPENRLPAAY+DGFNALMWLKQQAI+ P EHKWWLGNCNPS LFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPS-TSALTLATA 240
           ANI+YN ATR G NE   LKPLTLKG+ILIQPFFGGESRTKSEKLAA PS TSALTL+ A
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 300
           DTYWRLSLPP A+RDHPWSNPLVNGG KL++ KLPP MVCISELDIL+DRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
           AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 348

BLAST of MC08g1877 vs. NCBI nr
Match: XP_038904086.1 (probable carboxylesterase 17 [Benincasa hispida])

HSP 1 Score: 595 bits (1535), Expect = 7.04e-213
Identity = 292/341 (85.63%), Postives = 307/341 (90.03%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MA ISIDPRLKLQMPK H+Q  +T EEIEGLIRVYADGHVER PIIPNV C+TTAS+KGV
Sbjct: 17  MATISIDPRLKLQMPKLHIQNEATVEEIEGLIRVYADGHVERFPIIPNVSCNTTASDKGV 76

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           +AKDVLIDNFTNTWARIY+ANSQR LPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC
Sbjct: 77  TAKDVLIDNFTNTWARIYVANSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 136

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
            ILS+NYRLSPENRLPAAYEDGFN LMWLKQ AI+ P EHKWWLGNCN S LFLAGDSAG
Sbjct: 137 VILSVNYRLSPENRLPAAYEDGFNTLMWLKQLAITNPIEHKWWLGNCNQSKLFLAGDSAG 196

Query: 181 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPST-SALTLATA 240
           ANIAYNIA R GLNEP  LKPL LKG+ILIQPFFGGESRTKSEKLA QPS+ SALTL  A
Sbjct: 197 ANIAYNIAARVGLNEPTILKPLNLKGIILIQPFFGGESRTKSEKLATQPSSASALTLDAA 256

Query: 241 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 300
           DTYWRLSLPPGA+RDH WSNPLVNGG KLR+TK+PP MVCISELDILVDRNLEFCAAM A
Sbjct: 257 DTYWRLSLPPGANRDHTWSNPLVNGGVKLRETKVPPLMVCISELDILVDRNLEFCAAMTA 316

Query: 301 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFI 340
           AGKKVERVI KGVGH FQILNKDQIS+ R +EMMV IQ FI
Sbjct: 317 AGKKVERVILKGVGHSFQILNKDQISSARRREMMVCIQRFI 357

BLAST of MC08g1877 vs. NCBI nr
Match: XP_023523365.1 (probable carboxylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592 bits (1527), Expect = 8.06e-212
Identity = 291/343 (84.84%), Postives = 309/343 (90.09%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAA+ +DPRLKLQM K HLQ   T+EEIEGLIRVYADGHVERPPIIPNV CSTTAS+KGV
Sbjct: 6   MAAMYVDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
            ILSINYRLSPENRLPAAY+DGFNALMWLKQQAI+ P EHKWW GNCNPS LFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWSGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPS-TSALTLATA 240
           ANI YN ATR G NE   LKPLTLKG+ILIQPFFGGESRTKSEKLAA PS TSALTL+ +
Sbjct: 186 ANIVYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 245

Query: 241 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 300
           DTYWRLSLPP A+RDHPWSNPLVNGG KL++TKLPP MVCISELDIL+DRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKETKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
           AGKKV+RVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 306 AGKKVDRVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 348

BLAST of MC08g1877 vs. ExPASy TrEMBL
Match: A0A6J1DL90 (probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4 SV=1)

HSP 1 Score: 698 bits (1801), Expect = 7.64e-254
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
           AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATAD 240
           ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATAD
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATAD 245

Query: 241 TYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAA 300
           TYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAA
Sbjct: 246 TYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAA 305

Query: 301 GKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
           GKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ
Sbjct: 306 GKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of MC08g1877 vs. ExPASy TrEMBL
Match: A0A6J1KCZ3 (probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV=1)

HSP 1 Score: 597 bits (1538), Expect = 6.84e-214
Identity = 295/343 (86.01%), Postives = 311/343 (90.67%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAA+ IDPRLKLQM K HLQ  +T+EEIEGLIRVYADGHVERPPIIPNV CSTTASNKGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
            ILSINYRLSPENRLPAAYEDGFNA+MWLKQQAI+ P EHKWWLGNCNPS LFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPS-TSALTLATA 240
           ANIAYN ATR G NE   LKP TLKG+ILIQPFFGGESRTKSEKLAA PS TSALTL+ +
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 300
           DTYWRLSLPP A+RDHPWSNPLVNGG KL++ KLPP MVCISELDIL+DRNLEFCAAMAA
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
           AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 343

BLAST of MC08g1877 vs. ExPASy TrEMBL
Match: A0A6J1G8X8 (probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 SV=1)

HSP 1 Score: 595 bits (1535), Expect = 2.36e-213
Identity = 294/343 (85.71%), Postives = 310/343 (90.38%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAA+ IDPRLKLQM K HLQ   T+EEIEGLIRVYADGHVERPPIIPNV CSTTAS+KGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
            ILSINYRLSPENRLPAAY+DGFNALMWLKQQAI+ P EHKWWLGNCNPS LFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPS-TSALTLATA 240
           ANI+YN ATR G NE   LKPLTLKG+ILIQPFFGGESRTKSEKLAA PS TSALTL+ A
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 300
           DTYWRLSLPP A+RDHPWSNPLVNGG KL++ KLPP MVCISELDIL+DRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
           AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 348

BLAST of MC08g1877 vs. ExPASy TrEMBL
Match: A0A2N9EQ59 (Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4850 PE=4 SV=1)

HSP 1 Score: 470 bits (1210), Expect = 5.87e-164
Identity = 227/345 (65.80%), Postives = 271/345 (78.55%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAAIS+DPR KLQ+ K+H Q     EEIEGLI+V  DGHVERPPI+P VPC T A  +GV
Sbjct: 1   MAAISLDPRFKLQVGKNHHQHGGVVEEIEGLIKVCMDGHVERPPIVPIVPC-TMALERGV 60

Query: 61  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 120
           +A DV+ID  T+ WARIY+ +   KLPLLVYFHGGGFCVGSAAWSCY+EFL +LASKA C
Sbjct: 61  TANDVVIDKCTSLWARIYVPSCSGKLPLLVYFHGGGFCVGSAAWSCYNEFLSNLASKANC 120

Query: 121 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 180
            I+S+NYRL+PENRLPAAYEDGFN LMW+KQQA++  NEHKWWL +CN +++FL GDS+G
Sbjct: 121 VIISVNYRLAPENRLPAAYEDGFNTLMWVKQQALNGSNEHKWWLRHCNLTSMFLGGDSSG 180

Query: 181 ANIAYNIATR---PGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLA 240
           ANIAYN+ATR    G +E   ++PL LKG ILIQPFFGGE+RT SEK   QP+ S LTL 
Sbjct: 181 ANIAYNVATRLGSSGTSESTIIRPLCLKGTILIQPFFGGEARTWSEKYTNQPANSGLTLL 240

Query: 241 TADTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAM 300
            +DTYWRLSLP G +RD+PW NPL NG  KLR+ +LP  MVC+SE DIL DRNLEFC A+
Sbjct: 241 VSDTYWRLSLPIGTNRDNPWCNPLANGMAKLREFRLPSIMVCVSETDILKDRNLEFCTAL 300

Query: 301 AAAGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
             AGK+VE VI+KGVGH FQIL+  Q+S  RT EMM  I+ FI++
Sbjct: 301 TNAGKRVETVIYKGVGHAFQILHNSQLSQPRTHEMMAHIKAFINE 344

BLAST of MC08g1877 vs. ExPASy TrEMBL
Match: F6HSF1 (Abhydrolase_3 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s0006g03180 PE=4 SV=1)

HSP 1 Score: 466 bits (1200), Expect = 1.81e-162
Identity = 227/343 (66.18%), Postives = 269/343 (78.43%), Query Frame = 0

Query: 1   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 60
           MAAIS+DPRL LQM K+  Q     E++EGLIRVY DGHVERP I+PNVPC T A   GV
Sbjct: 1   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPC-TVALELGV 60

Query: 61  SAKDVLIDNFTNTWARIYIANSQR-KLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAK 120
           + KDV+I+ ++N WAR Y+ +    KLPLLVYFHGGGFCVGSAAW+CYH FL DLASKA 
Sbjct: 61  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 120

Query: 121 CAILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSA 180
           C I+S+NYRL+PENRLPAAYEDGFNA+MW+K QA++   E KWWL  CN S+LFL GDSA
Sbjct: 121 CLIMSVNYRLAPENRLPAAYEDGFNAVMWVKNQALNGAGEQKWWLSRCNLSSLFLTGDSA 180

Query: 181 GANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATA 240
           GANIAYN+ATR G ++   LKPL+LKG ILIQPFFGGE+RT SE  + QP  SALTL+ +
Sbjct: 181 GANIAYNVATRLGSSDTTFLKPLSLKGTILIQPFFGGEARTGSENHSTQPPNSALTLSAS 240

Query: 241 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 300
           DTYWRLSLP GA+RDHP  NPL NG TKLR  +LPP+MVCIS+ DIL DRNL+FC AMA 
Sbjct: 241 DTYWRLSLPLGANRDHPCCNPLANGSTKLRTLQLPPTMVCISDTDILKDRNLQFCTAMAN 300

Query: 301 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 342
           AGK++E VI+KGVGH FQ+L    +S  RT+EM+  I+ FI Q
Sbjct: 301 AGKRLETVIYKGVGHAFQVLQNSDLSQPRTKEMISHIRAFITQ 342

BLAST of MC08g1877 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 350.1 bits (897), Expect = 2.0e-96
Identity = 179/329 (54.41%), Postives = 223/329 (67.78%), Query Frame = 0

Query: 18  HLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGVSAKDVLIDNFTNTWARI 77
           H +     EEIEGLI+V+ DG VERPPI+P V  +   S+K  +A D+ + N  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFDIKLSN--DTWTRV 81

Query: 78  YI-----ANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPE 137
           YI     A+    LPLLVYFHGGGFCVGSAAWSCYH+FL  LA KA+C I+S+NYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 138 NRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAGANIAYNIATRPG 197
           +RLPAAY+DG N + WL +Q IS    +  WL  CN SN+FLAGDSAGANIAY +A R  
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI- 201

Query: 198 LNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGAS 257
           +        L LKG+ILI PFFGGESRT SEK      +SALTL+ +D YWRL+LP GAS
Sbjct: 202 MASGKYANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGAS 261

Query: 258 RDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAAGKKVERVIFKGV 317
           RDHPW NPL++        KLP +MV ++E DIL +RNLE C  M + GK+VE ++  GV
Sbjct: 262 RDHPWCNPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGV 321

Query: 318 GHGFQILNKDQISAIRTQEMMVSIQNFIH 342
           GH F IL+   +S  R  +MM  + NFIH
Sbjct: 322 GHAFHILDNSSVSRDRIHDMMCRLHNFIH 342

BLAST of MC08g1877 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 307.0 bits (785), Expect = 1.9e-83
Identity = 151/321 (47.04%), Postives = 211/321 (65.73%), Query Frame = 0

Query: 26  EEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGVSAKDVLIDNFTNTWARIYI-----A 85
           +E+EGLI+VY DGHVER  ++P V  S      GV+  DV+ID  TN WAR+Y+      
Sbjct: 25  DEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVPMTTTK 84

Query: 86  NSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPENRLPAAYE 145
           +S  KLPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C ++S+NYRL+PEN LPAAYE
Sbjct: 85  SSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYE 144

Query: 146 DGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAGANIAYNIATRPGLNEPATLK 205
           DG NA++WL     ++      W   C+   +FLAGDSAG NIA  +A R    E   LK
Sbjct: 145 DGVNAILWL-----NKARNDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPEDLALK 204

Query: 206 PLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGASRDHPWSNP 265
              ++G ILIQPF+ GE RT+SE+      T+ LTLA++D +WR+SLP GA+R+HP+  P
Sbjct: 205 ---IEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKP 264

Query: 266 LVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAAAGKKVERVIFKGVGHGFQILN 325
           +      ++ + +  ++VC++E+D+L+D N+E C         ++RV+ KGVGH F IL 
Sbjct: 265 V---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDV---IKRVLHKGVGHAFHILG 324

Query: 326 KDQISAIRTQEMMVSIQNFIH 342
           K Q++   T EM+  I  FIH
Sbjct: 325 KSQLAHTTTLEMLCQIDAFIH 330

BLAST of MC08g1877 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.8e-50
Identity = 114/298 (38.26%), Postives = 170/298 (57.05%), Query Frame = 0

Query: 25  AEEIEGLIRVYADGHVERPPIIPNVPCSTT-ASNKGVSAKDVLIDNFTNTWARIY---IA 84
           AE+  GL+++ ++G V R   I  +       +N+ V  KD +     N   R+Y    A
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  NSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPENRLPAAYE 144
           +++  LP++V+FHGGGFC GS +W  +H F L LAS     ++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAISEPNEHKWWLG-NCNPSNLFLAGDSAGANIAYNIATRPGLNEPATL 204
           D    L WL  QA+S+   H +  G + +   +F+ GDS+G NIA+ +A R G +    L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGASRDHPWSN 264
            P+ ++G +L+ PFFGGE RT SE     PS + L+L   D +WRLSLP GA+RDH  +N
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 250

Query: 265 PLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAM-AAAGKKVERVIFKGVGHGF 317
           P       L    L P +V +   ++L DR  E+   +    GK+V+ + F+   HGF
Sbjct: 251 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of MC08g1877 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.8e-50
Identity = 114/298 (38.26%), Postives = 170/298 (57.05%), Query Frame = 0

Query: 25  AEEIEGLIRVYADGHVERPPIIPNVPCSTT-ASNKGVSAKDVLIDNFTNTWARIY---IA 84
           AE+  GL+++ ++G V R   I  +       +N+ V  KD +     N   R+Y    A
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  NSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPENRLPAAYE 144
           +++  LP++V+FHGGGFC GS +W  +H F L LAS     ++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAISEPNEHKWWLG-NCNPSNLFLAGDSAGANIAYNIATRPGLNEPATL 204
           D    L WL  QA+S+   H +  G + +   +F+ GDS+G NIA+ +A R G +    L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGASRDHPWSN 264
            P+ ++G +L+ PFFGGE RT SE     PS + L+L   D +WRLSLP GA+RDH  +N
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 250

Query: 265 PLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAM-AAAGKKVERVIFKGVGHGF 317
           P       L    L P +V +   ++L DR  E+   +    GK+V+ + F+   HGF
Sbjct: 251 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of MC08g1877 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 174.9 bits (442), Expect = 1.1e-43
Identity = 109/320 (34.06%), Postives = 170/320 (53.12%), Query Frame = 0

Query: 37  DGHVERPPIIPNVPCSTTAS-----NKGVSAKDVLIDNFTNTWARIYIAN--------SQ 96
           DG + R   + N PC+         N  VS KD+ ++   +TW R+Y+ +        S 
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVS-KDLPVNQLKSTWLRLYLPSSAVNEGNVSS 80

Query: 97  RKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKCAILSINYRLSPENRLPAAYEDGF 156
           +KLP++VY+HGGGF + S     +H+F  ++A      ++S +YRL+PE+RLPAAY+DG 
Sbjct: 81  QKLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGV 140

Query: 157 NALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAGANIAYNIATRPGLNEPATLKPLT 216
            AL W+K       ++ +W   + + SN+FL G SAG N+AYN+  R  ++  + L PL 
Sbjct: 141 EALDWIK------TSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLR-SVDSVSDLSPLQ 200

Query: 217 LKGVILIQPFFGGESRTKSEKLAAQPSTSALTLATADTYWRLSLPPGASRDHPWSNPLVN 276
           ++G+IL  PFFGGE R++SE      +         D  W LSLP G  RDH +SNP V 
Sbjct: 201 IRGLILHHPFFGGEERSESE--IRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 277 GGTKLRDT--KLPPSMVCI-SELDILVDRNLEFCAAMAAAGKKVERVIFKGVGHGFQILN 336
            G++  +   +L   ++ I  E D ++D   +    M   G +V      G  HG +I +
Sbjct: 261 DGSEKLEKIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIRD 320

Query: 337 KDQISAIRTQEMMVSIQNFI 341
                  + + + +SI+NFI
Sbjct: 321 PS-----KRKTLFLSIKNFI 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFR72.8e-9554.41Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9SX252.7e-8247.04Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9FG133.9e-4938.26Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q9LVB81.6e-4234.06Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1[more]
Q0ZPV78.8e-4132.26Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022155020.11.58e-253100.00probable carboxylesterase 17 [Momordica charantia][more]
XP_022998605.11.41e-21386.01probable carboxylesterase 17 [Cucurbita maxima][more]
XP_022948283.14.87e-21385.71probable carboxylesterase 17 [Cucurbita moschata][more]
XP_038904086.17.04e-21385.63probable carboxylesterase 17 [Benincasa hispida][more]
XP_023523365.18.06e-21284.84probable carboxylesterase 17 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DL907.64e-254100.00probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4... [more]
A0A6J1KCZ36.84e-21486.01probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV... [more]
A0A6J1G8X82.36e-21385.71probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 ... [more]
A0A2N9EQ595.87e-16465.80Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS... [more]
F6HSF11.81e-16266.18Abhydrolase_3 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s000... [more]
Match NameE-valueIdentityDescription
AT5G16080.12.0e-9654.41carboxyesterase 17 [more]
AT1G68620.11.9e-8347.04alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.12.8e-5038.26alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.22.8e-5038.26alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.11.1e-4334.06carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 89..317
e-value: 5.1E-56
score: 189.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 17..341
e-value: 5.3E-78
score: 264.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 24..321
NoneNo IPR availablePANTHERPTHR23024:SF409CARBOXYLESTERASE 6-RELATEDcoord: 14..340
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 14..340
IPR033140Lipase, GDXG, putative serine active sitePROSITEPS01174LIPASE_GDXG_SERcoord: 172..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1877.1MC08g1877.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity