MC08g1837 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1837
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description2-hydroxyacyl-CoA lyase
LocationMC08: 26627585 .. 26630691 (-)
RNA-Seq ExpressionMC08g1837
SyntenyMC08g1837
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATTTTCGTCAACGTGGAAACAGTGGAAATTCCGTACCGGCGATTTATAAATTCACTCGACAATTAAATCGGGCTTCGTAATCGTATCTCAAAATTATTTAATTCCCCTTCACTCTCTCTCTGTACACTACATTTTCCGACCGGCCCACCGGGAAATCTCAGCTCCGATGGGGGATTCCGCCGGAGATCTTCAGAATGACCTCAAATTTCACGGTCCGATCGACGGAAACGTCCTCGCCGCCATGGCGCTCGCCCGCTGCGGCGTCGACCGGATGTTCGGCGTCGTCGGCATCCCCGTTACCTCTCTGGCCACTCGCGCCGTCTCGCTCGGGATCCGATTCATCGCTTTCCACAACGAACAGTCGGCTGGCTATGCCGCTTCTGCTTATGGCTATCTAACGGGTCGCCCTGGTGTGCTTCTCACTGTCTCTGGCCCCGGATGTGTCCATGGCCTCGCGGGGGTTTCCAACGCCATGGCTAATGCTTGGCCCATGGTCATGATCTCTGGCTCCTGTGATCAGAGAGATTTCGGCCGTGGTGATTTTCAGGAGCTTGATCAAATCGAAGCTGTGAAACCCTTTTCGAAATTCTCCGTCAAGGCCAAGGACATCTCCGAGATTCCGAATTGCGTCGCCCAGGTTCTTAGTTCCGCCGCTTCAGGCCGGCCCGGCGGGTGCTACTTTGATCTTCCGTCGGATGTTCTGCACCAGACAGTTTCCGAGTCTGAAGCCGAGAGATTATTGACCGAAGCGGAGAAATCTGCTCGCCGCGAGGAGATTCACAGAGTTCCTAATTCGCAGATTCAGGAAGCAATTTCGTTGCTGAAAAACGCGGAGAGGCCATTGATCGTGTTCGGTAAAGGAGCGGCGATTGCGCGGGCTGAGGGGCCGCTGAAGAAGCTCGTGGAGAGCACTGGAATCCCATTTCTCCCGACTCCAATGGGGAAAGGCTTGCTGTCCGATACGCACGAGCTCGCGGCCACTGCCGCGAGGTCCTTAGCCATTGGAAAGTGCGATGTTGCGCTTGTGGTTGGGGCGAGGCTTAATTGGCTGTTGCACTTTGGGGAGCCGCCTAAATGGTCCAAGGACGTGAAGTTCATTTTGGTTGATATTTCCGAGGAAGAGGTTGAGTTAAGAAAGCCCCGTTTGGGTTTGATCGGAGATGCCAAACAGGTCTTGGAATCCATAAACAAGGAGATTAAGGATGACCCATTTTCTCTTGGGAAGTCTCACCCATGGGTGGAAGCCATTTCGCAGAAGGCAAAAGAAAATGTGTCGAAAATGGAAGTTCAGTTGGCGAGGGATGTTGTGCCATTCAATTTCTTGACGCCAATGAGAATCATTAGAGATGCCATTTCGGCTCAGGGTAGCCCTGCTCCCATATTGGTTTCTGAAGGGGCCAATACAATGGATGTTGGTCGCTCAGTTTTGGTTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACGTGGGGGACAATGGGGGTTGGCTTGGGCTATTGCATTGCTGCTGCAGTTGCTTCGCCCGATCGATTGGTAGTTGCCGTGGAAGGCGATTCTGGATTCGGGTTTAGCGCCATGGAAGTTGAGGTATCCATTTCTCCCTTTTCTTTTATCCTTCACCATTACGTATACTCACAATTGATTAGAATGTTGTATGATCAATGTCTTCGTCTGTCTGTACTAATTCCAATTGAAGACTGTTCTGTATATTAAAAGCATTGTTTGGTTGCCTCAAAATGTTGCCTCAAGTCAATCATCATGGTTAGAGTTACTTCTTGGTTTGCAAATGTCTGTTTGTCCATTAGAAACATGGTAAAGGATTGGTTAGTATGTTTTATTTGAGGTCAAAAGACATGTGTTGGTCTCTGGTAATCAGTGGTTTTTAGAATTATTCTAGGACATGTTTCATAGCCTCTCTTGAAAGGAGTTACCTCCTTGTTATGGCGGTTGGGTTTTAGATGACATTCTATTTTCTTTTTAGGTTCTGTTTGTTGTTTGTCTCACAGTGAAGCATTAGATTTCCCATGTTTTTCTGCACTTATATTAGGTAGGCCCTTGTATTTTTACTATTTTACTCGTTTTATCACCATAGAGTATTCGGACTCAACTAGTCTCACAAAATAACTACCTTACTACAATATTGACATCGAGATAATATCTTAGGTCTTAGGTCAGTGGCTACCATAGTTGACGTATAAATATTTATTAATTCACTTGTTAATAGGGCCAGTCTTGTTAATAGGGCATCAATGGATCTGACGAACAAAATTATGTACAGATCACCATCTTTTAAATCTGAAATTTAGATTTTGCTCCCAATCTGGCTGATGATTTTTTTAACCTATGTTGAAGTTCTAGATGTTGTTTCTGTATCTGATTTTTGTCTGTAGAGCTGTACATCTTACTTTGGTTTTGGGCTTCATTGTTGTAGAATTAGATTATTCCTTGTGTTCTTTCTGAAGAGCTCCTTATAATCTTTGGAGTTCATATATTAAGTTCCAAAACATATTTGCAATTGAGATTTCAGTAACAAACAGATGGTTTCATTTTCATTTCCTCTTTACAAATTTCTAATCTTGCTCATGCTTTTTTTTGAACTTAAACAGACGCTGGTTCGATACCAGTTGCCAGTAGTTGTGATAGTTTTCAACAATGGCGGTGTATATGGCGGTGACCGGAGAACGCCACAAGAAATCACCGGACCCTATAAGCATGACCCTGCGCCCACCTCATTCGTCCCATCAGCAAATTACCACAAAATGATCGAGGCTTTCGGAGGAAAAGGATACTATGTCGAAACACCCGAAGAACTAAAATCCGCACTAGCTGAGTCCTTTTCTGCTCGAAAACCAGCAGTCGTAAACGTCAAGATCGATCCTTTTGCCGGTGCGGAAAGTGGGAGGCTGCAACATAAGAACTGAAGATAAGTCTTTGAACTCCTTTGTTGTTTGTTCTTCACCTGAGAGACTCATTTCTGCCAATCAATTAGGTTTGCAGCAGAGTTTCAAGCAAAGCTTAGGATTTTTTTTTTTCCACAATATTATGTGAAATTATGAACTTGAATAAAGTTATATGTAACTTGTAAAGCCAGCAGTTGGAGATGT

mRNA sequence

GTATTTTCGTCAACGTGGAAACAGTGGAAATTCCGTACCGGCGATTTATAAATTCACTCGACAATTAAATCGGGCTTCGTAATCGTATCTCAAAATTATTTAATTCCCCTTCACTCTCTCTCTGTACACTACATTTTCCGACCGGCCCACCGGGAAATCTCAGCTCCGATGGGGGATTCCGCCGGAGATCTTCAGAATGACCTCAAATTTCACGGTCCGATCGACGGAAACGTCCTCGCCGCCATGGCGCTCGCCCGCTGCGGCGTCGACCGGATGTTCGGCGTCGTCGGCATCCCCGTTACCTCTCTGGCCACTCGCGCCGTCTCGCTCGGGATCCGATTCATCGCTTTCCACAACGAACAGTCGGCTGGCTATGCCGCTTCTGCTTATGGCTATCTAACGGGTCGCCCTGGTGTGCTTCTCACTGTCTCTGGCCCCGGATGTGTCCATGGCCTCGCGGGGGTTTCCAACGCCATGGCTAATGCTTGGCCCATGGTCATGATCTCTGGCTCCTGTGATCAGAGAGATTTCGGCCGTGGTGATTTTCAGGAGCTTGATCAAATCGAAGCTGTGAAACCCTTTTCGAAATTCTCCGTCAAGGCCAAGGACATCTCCGAGATTCCGAATTGCGTCGCCCAGGTTCTTAGTTCCGCCGCTTCAGGCCGGCCCGGCGGGTGCTACTTTGATCTTCCGTCGGATGTTCTGCACCAGACAGTTTCCGAGTCTGAAGCCGAGAGATTATTGACCGAAGCGGAGAAATCTGCTCGCCGCGAGGAGATTCACAGAGTTCCTAATTCGCAGATTCAGGAAGCAATTTCGTTGCTGAAAAACGCGGAGAGGCCATTGATCGTGTTCGGTAAAGGAGCGGCGATTGCGCGGGCTGAGGGGCCGCTGAAGAAGCTCGTGGAGAGCACTGGAATCCCATTTCTCCCGACTCCAATGGGGAAAGGCTTGCTGTCCGATACGCACGAGCTCGCGGCCACTGCCGCGAGGTCCTTAGCCATTGGAAAGTGCGATGTTGCGCTTGTGGTTGGGGCGAGGCTTAATTGGCTGTTGCACTTTGGGGAGCCGCCTAAATGGTCCAAGGACGTGAAGTTCATTTTGGTTGATATTTCCGAGGAAGAGGTTGAGTTAAGAAAGCCCCGTTTGGGTTTGATCGGAGATGCCAAACAGGTCTTGGAATCCATAAACAAGGAGATTAAGGATGACCCATTTTCTCTTGGGAAGTCTCACCCATGGGTGGAAGCCATTTCGCAGAAGGCAAAAGAAAATGTGTCGAAAATGGAAGTTCAGTTGGCGAGGGATGTTGTGCCATTCAATTTCTTGACGCCAATGAGAATCATTAGAGATGCCATTTCGGCTCAGGGTAGCCCTGCTCCCATATTGGTTTCTGAAGGGGCCAATACAATGGATGTTGGTCGCTCAGTTTTGGTTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACGTGGGGGACAATGGGGGTTGGCTTGGGCTATTGCATTGCTGCTGCAGTTGCTTCGCCCGATCGATTGGTAGTTGCCGTGGAAGGCGATTCTGGATTCGGGTTTAGCGCCATGGAAGTTGAGACGCTGGTTCGATACCAGTTGCCAGTAGTTGTGATAGTTTTCAACAATGGCGGTGTATATGGCGGTGACCGGAGAACGCCACAAGAAATCACCGGACCCTATAAGCATGACCCTGCGCCCACCTCATTCGTCCCATCAGCAAATTACCACAAAATGATCGAGGCTTTCGGAGGAAAAGGATACTATGTCGAAACACCCGAAGAACTAAAATCCGCACTAGCTGAGTCCTTTTCTGCTCGAAAACCAGCAGTCGTAAACGTCAAGATCGATCCTTTTGCCGGTGCGGAAAGTGGGAGGCTGCAACATAAGAACTGAAGATAAGTCTTTGAACTCCTTTGTTGTTTGTTCTTCACCTGAGAGACTCATTTCTGCCAATCAATTAGGTTTGCAGCAGAGTTTCAAGCAAAGCTTAGGATTTTTTTTTTTCCACAATATTATGTGAAATTATGAACTTGAATAAAGTTATATGTAACTTGTAAAGCCAGCAGTTGGAGATGT

Coding sequence (CDS)

ATGGGGGATTCCGCCGGAGATCTTCAGAATGACCTCAAATTTCACGGTCCGATCGACGGAAACGTCCTCGCCGCCATGGCGCTCGCCCGCTGCGGCGTCGACCGGATGTTCGGCGTCGTCGGCATCCCCGTTACCTCTCTGGCCACTCGCGCCGTCTCGCTCGGGATCCGATTCATCGCTTTCCACAACGAACAGTCGGCTGGCTATGCCGCTTCTGCTTATGGCTATCTAACGGGTCGCCCTGGTGTGCTTCTCACTGTCTCTGGCCCCGGATGTGTCCATGGCCTCGCGGGGGTTTCCAACGCCATGGCTAATGCTTGGCCCATGGTCATGATCTCTGGCTCCTGTGATCAGAGAGATTTCGGCCGTGGTGATTTTCAGGAGCTTGATCAAATCGAAGCTGTGAAACCCTTTTCGAAATTCTCCGTCAAGGCCAAGGACATCTCCGAGATTCCGAATTGCGTCGCCCAGGTTCTTAGTTCCGCCGCTTCAGGCCGGCCCGGCGGGTGCTACTTTGATCTTCCGTCGGATGTTCTGCACCAGACAGTTTCCGAGTCTGAAGCCGAGAGATTATTGACCGAAGCGGAGAAATCTGCTCGCCGCGAGGAGATTCACAGAGTTCCTAATTCGCAGATTCAGGAAGCAATTTCGTTGCTGAAAAACGCGGAGAGGCCATTGATCGTGTTCGGTAAAGGAGCGGCGATTGCGCGGGCTGAGGGGCCGCTGAAGAAGCTCGTGGAGAGCACTGGAATCCCATTTCTCCCGACTCCAATGGGGAAAGGCTTGCTGTCCGATACGCACGAGCTCGCGGCCACTGCCGCGAGGTCCTTAGCCATTGGAAAGTGCGATGTTGCGCTTGTGGTTGGGGCGAGGCTTAATTGGCTGTTGCACTTTGGGGAGCCGCCTAAATGGTCCAAGGACGTGAAGTTCATTTTGGTTGATATTTCCGAGGAAGAGGTTGAGTTAAGAAAGCCCCGTTTGGGTTTGATCGGAGATGCCAAACAGGTCTTGGAATCCATAAACAAGGAGATTAAGGATGACCCATTTTCTCTTGGGAAGTCTCACCCATGGGTGGAAGCCATTTCGCAGAAGGCAAAAGAAAATGTGTCGAAAATGGAAGTTCAGTTGGCGAGGGATGTTGTGCCATTCAATTTCTTGACGCCAATGAGAATCATTAGAGATGCCATTTCGGCTCAGGGTAGCCCTGCTCCCATATTGGTTTCTGAAGGGGCCAATACAATGGATGTTGGTCGCTCAGTTTTGGTTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACGTGGGGGACAATGGGGGTTGGCTTGGGCTATTGCATTGCTGCTGCAGTTGCTTCGCCCGATCGATTGGTAGTTGCCGTGGAAGGCGATTCTGGATTCGGGTTTAGCGCCATGGAAGTTGAGACGCTGGTTCGATACCAGTTGCCAGTAGTTGTGATAGTTTTCAACAATGGCGGTGTATATGGCGGTGACCGGAGAACGCCACAAGAAATCACCGGACCCTATAAGCATGACCCTGCGCCCACCTCATTCGTCCCATCAGCAAATTACCACAAAATGATCGAGGCTTTCGGAGGAAAAGGATACTATGTCGAAACACCCGAAGAACTAAAATCCGCACTAGCTGAGTCCTTTTCTGCTCGAAAACCAGCAGTCGTAAACGTCAAGATCGATCCTTTTGCCGGTGCGGAAAGTGGGAGGCTGCAACATAAGAACTGA

Protein sequence

MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRDFGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLHQTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEAISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN
Homology
BLAST of MC08g1837 vs. ExPASy Swiss-Prot
Match: Q9LF46 (2-hydroxyacyl-CoA lyase OS=Arabidopsis thaliana OX=3702 GN=HACL PE=1 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 2.8e-265
Identity = 452/561 (80.57%), Postives = 501/561 (89.30%), Query Frame = 0

Query: 18  IDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 77
           IDGNVL A +L+  GV  MFGVVGIPVTSLA+RA++LGIRFIAFHNEQSAGYAASAYGYL
Sbjct: 12  IDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMALGIRFIAFHNEQSAGYAASAYGYL 71

Query: 78  TGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRDFGRGDFQELDQIEAVKP 137
           TG+PG+LLTVSGPGCVHGLAG+SNA  N WPMVMISGSCDQRD GRGDFQELDQIEAVK 
Sbjct: 72  TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQIEAVKA 131

Query: 138 FSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLHQTVSESEAERLLTEAEK 197
           FSK S KAKD+ EIP+CV++VL  A SGRPGGCY D+P+DVL Q +SESEA++L+ E E+
Sbjct: 132 FSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKLVDEVER 191

Query: 198 SARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTP 257
           S + E I     S+I+ A+SLL+ AERPLIVFGKGAA +RAE  LKKLVE TGIPFLPTP
Sbjct: 192 SRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLPTP 251

Query: 258 MGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDISE 317
           MGKGLL DTHE +ATAARSLAIGKCDVALVVGARLNWLLHFGE PKW KDVKFILVD+SE
Sbjct: 252 MGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVKFILVDVSE 311

Query: 318 EEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEAISQKAKENVSKMEVQLA 377
           EE+ELRKP LG++GDAK V+  +N+EIKDDPF LGKS+ WVE+IS+KAKEN  KME+QLA
Sbjct: 312 EEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEKMEIQLA 371

Query: 378 RDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGT 437
           +DVVPFNFLTPMRIIRDAI A   P+P++VSEGANTMDVGRSVLVQ EPRTRLDAGTWGT
Sbjct: 372 KDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGT 431

Query: 438 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYGGD 497
           MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY L VV+IVFNNGGVYGGD
Sbjct: 432 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNGGVYGGD 491

Query: 498 RRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALAESFSARKPA 557
           RR P+EI+GP+K DPAPTSFVP+A YHK+IEAFGGKGY VETP+ELKSALAESF+ARKPA
Sbjct: 492 RRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESFAARKPA 551

Query: 558 VVNVKIDPFAGAESGRLQHKN 579
           VVNV IDPFAGAESGRLQHKN
Sbjct: 552 VVNVIIDPFAGAESGRLQHKN 572

BLAST of MC08g1837 vs. ExPASy Swiss-Prot
Match: Q0JMH0 (2-hydroxyacyl-CoA lyase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0505400 PE=3 SV=3)

HSP 1 Score: 865.9 bits (2236), Expect = 2.5e-250
Identity = 422/565 (74.69%), Postives = 490/565 (86.73%), Query Frame = 0

Query: 18  IDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 77
           +DG+ LA  ALA  G   MFGVVGIPVTSLA+RA + G+RF+AF NEQSAGYAA+AYG+L
Sbjct: 13  VDGSALAGRALAAAGARHMFGVVGIPVTSLASRAAAAGVRFLAFRNEQSAGYAAAAYGFL 72

Query: 78  TGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRDFGRGDFQELDQIEAVKP 137
           TG PG+LLTVSGPGCVHGLAG+S+A ANAWP++M+SGSC Q D GRGDFQELDQI A KP
Sbjct: 73  TGSPGLLLTVSGPGCVHGLAGLSHATANAWPLLMVSGSCSQPDAGRGDFQELDQIAATKP 132

Query: 138 FSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLHQTVSESEAERLL----T 197
           F K +VKA  I++IP  V Q L++  SGRPGGCY D+PSDVLHQT++ESEA  L+     
Sbjct: 133 FIKIAVKATTIADIPRLVFQALAATVSGRPGGCYLDIPSDVLHQTLTESEAAALIDAAAA 192

Query: 198 EAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVESTGIPF 257
           ++ KS      H+  +  I++A  LL+ AERPL+VFGKGAA +RAE  + KLV++TGIPF
Sbjct: 193 DSAKSDSSPPKHKSLDEGIEKAAELLRRAERPLVVFGKGAAYSRAEDAIWKLVDTTGIPF 252

Query: 258 LPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILV 317
           LPTPMGKG++ DTH L+ATAARSLAIG+CDVALVVGARLNWLLHFGEPPKWSKDVKFILV
Sbjct: 253 LPTPMGKGVVPDTHPLSATAARSLAIGQCDVALVVGARLNWLLHFGEPPKWSKDVKFILV 312

Query: 318 DISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEAISQKAKENVSKME 377
           D+ EEE+ELRKP +G++GDAK+V+E IN+EIKD PF L  SHPWVEAI++KA++NV KME
Sbjct: 313 DVCEEEIELRKPHVGIVGDAKRVVELINREIKDQPFCLAPSHPWVEAITKKARDNVLKME 372

Query: 378 VQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAG 437
            QLA+DVVPFNFLTP+RIIRDAI A+G+PAP++VSEGANTMDVGR+VLVQ EPRTRLDAG
Sbjct: 373 AQLAKDVVPFNFLTPLRIIRDAILAEGNPAPVVVSEGANTMDVGRAVLVQNEPRTRLDAG 432

Query: 438 TWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGV 497
           TWGTMGVGLG+C+AAAVA PDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNN GV
Sbjct: 433 TWGTMGVGLGFCVAAAVAEPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNNGV 492

Query: 498 YGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALAESFSA 557
           YGGDRR+P EITGPYK DPAPTSFVP+A YHKM+EAFGGKGY VETP+ELKSAL+ESF A
Sbjct: 493 YGGDRRSPDEITGPYKDDPAPTSFVPAAGYHKMMEAFGGKGYLVETPDELKSALSESFRA 552

Query: 558 RKPAVVNVKIDPFAGAESGRLQHKN 579
           RKPAV+NV IDP+AGAESGR+QHKN
Sbjct: 553 RKPAVINVIIDPYAGAESGRMQHKN 577

BLAST of MC08g1837 vs. ExPASy Swiss-Prot
Match: P0AFI1 (Oxalyl-CoA decarboxylase OS=Escherichia coli O157:H7 OX=83334 GN=oxc PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-127
Identity = 244/563 (43.34%), Postives = 359/563 (63.77%), Query Frame = 0

Query: 19  DGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLT 78
           DG  +   AL +  +D ++GVVGIPVT +A  A + GIR+I F +EQSAGYAA+A G+LT
Sbjct: 9   DGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAASGFLT 68

Query: 79  GRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQR--DFGRGDFQELDQIEAVK 138
            +PG+ LTVS PG ++GL  ++NA  N +PM+MISGS D+   D  +GD++ELDQ+ A K
Sbjct: 69  QKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELDQMNAAK 128

Query: 139 PFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLHQTVSESEAERLLTEAE 198
           P++K + +     ++   +A+ +  + SGRPGG Y DLP++VL  T+ + EA   + + E
Sbjct: 129 PYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEALTTIVKVE 188

Query: 199 KSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPT 258
             +    +   P S +  AISLL  AERPLI+ GKGAA ++A+  L++ +ES  IPFLP 
Sbjct: 189 NPS--PALLPCPKS-VTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESAQIPFLPM 248

Query: 259 PMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDIS 318
            M KG+L DTH L+A AARS A+   DV ++VGARLNWLL  G+   W+ D +FI +DI 
Sbjct: 249 SMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGK-KGWAADTQFIQLDIE 308

Query: 319 EEEVELRKP-RLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEAISQKAKENVSKMEVQ 378
            +E++  +P  + ++GD    ++ +  E+K + F+      W + ++   ++N  KM  +
Sbjct: 309 PQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFT--TPLVWRDILNIHKQQNAQKMHEK 368

Query: 379 LARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTW 438
           L+ D  P N+   +  +RD +  + +    LV+EGANT+D  R+++   +PR RLD GTW
Sbjct: 369 LSTDTQPLNYFNALSAVRDVL--RENQDIYLVNEGANTLDNARNIIDMYKPRRRLDCGTW 428

Query: 439 GTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYG 498
           G MG+G+GY I A+V S    VVA+EGDS FGFS ME+ET+ RY LPV +++FNNGG+Y 
Sbjct: 429 GVMGIGMGYAIGASVTSGSP-VVAIEGDSAFGFSGMEIETICRYNLPVTIVIFNNGGIYR 488

Query: 499 GDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALAESFSARK 558
           GD     +++G     P+PT  +  A Y K+++AF G GY V T +EL+ AL     +RK
Sbjct: 489 GD---GVDLSG--AGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTTGIQSRK 548

Query: 559 PAVVNVKIDPFAGAESGRLQHKN 579
           P ++NV IDP AG ESG +   N
Sbjct: 549 PTIINVVIDPAAGTESGHITKLN 557

BLAST of MC08g1837 vs. ExPASy Swiss-Prot
Match: P0AFI0 (Oxalyl-CoA decarboxylase OS=Escherichia coli (strain K12) OX=83333 GN=oxc PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-127
Identity = 244/563 (43.34%), Postives = 359/563 (63.77%), Query Frame = 0

Query: 19  DGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLT 78
           DG  +   AL +  +D ++GVVGIPVT +A  A + GIR+I F +EQSAGYAA+A G+LT
Sbjct: 9   DGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAASGFLT 68

Query: 79  GRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQR--DFGRGDFQELDQIEAVK 138
            +PG+ LTVS PG ++GL  ++NA  N +PM+MISGS D+   D  +GD++ELDQ+ A K
Sbjct: 69  QKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELDQMNAAK 128

Query: 139 PFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLHQTVSESEAERLLTEAE 198
           P++K + +     ++   +A+ +  + SGRPGG Y DLP++VL  T+ + EA   + + E
Sbjct: 129 PYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEALTTIVKVE 188

Query: 199 KSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPT 258
             +    +   P S +  AISLL  AERPLI+ GKGAA ++A+  L++ +ES  IPFLP 
Sbjct: 189 NPS--PALLPCPKS-VTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESAQIPFLPM 248

Query: 259 PMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDIS 318
            M KG+L DTH L+A AARS A+   DV ++VGARLNWLL  G+   W+ D +FI +DI 
Sbjct: 249 SMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGK-KGWAADTQFIQLDIE 308

Query: 319 EEEVELRKP-RLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEAISQKAKENVSKMEVQ 378
            +E++  +P  + ++GD    ++ +  E+K + F+      W + ++   ++N  KM  +
Sbjct: 309 PQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFT--TPLVWRDILNIHKQQNAQKMHEK 368

Query: 379 LARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTW 438
           L+ D  P N+   +  +RD +  + +    LV+EGANT+D  R+++   +PR RLD GTW
Sbjct: 369 LSTDTQPLNYFNALSAVRDVL--RENQDIYLVNEGANTLDNARNIIDMYKPRRRLDCGTW 428

Query: 439 GTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYG 498
           G MG+G+GY I A+V S    VVA+EGDS FGFS ME+ET+ RY LPV +++FNNGG+Y 
Sbjct: 429 GVMGIGMGYAIGASVTSGSP-VVAIEGDSAFGFSGMEIETICRYNLPVTIVIFNNGGIYR 488

Query: 499 GDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALAESFSARK 558
           GD     +++G     P+PT  +  A Y K+++AF G GY V T +EL+ AL     +RK
Sbjct: 489 GD---GVDLSG--AGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTTGIQSRK 548

Query: 559 PAVVNVKIDPFAGAESGRLQHKN 579
           P ++NV IDP AG ESG +   N
Sbjct: 549 PTIINVVIDPAAGTESGHITKLN 557

BLAST of MC08g1837 vs. ExPASy Swiss-Prot
Match: Q8CHM7 (2-hydroxyacyl-CoA lyase 1 OS=Rattus norvegicus OX=10116 GN=Hacl1 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 5.7e-125
Identity = 242/558 (43.37%), Postives = 350/558 (62.72%), Query Frame = 0

Query: 18  IDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 77
           + G  + A AL    V+ MFGVVGIPVT +A  A  LGI++I   NEQ+A YAASA GYL
Sbjct: 17  VSGAKVIAQALKTQDVEYMFGVVGIPVTEIALAAQELGIKYIGMRNEQAACYAASAVGYL 76

Query: 78  TGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRDFGRGDFQELDQIEAVKP 137
           TGRPGV L VSGPG +H L G++NA  N WP+++I GS ++     G FQE  Q+EA + 
Sbjct: 77  TGRPGVCLVVSGPGLIHALGGMANANMNCWPLIVIGGSSERNQEAMGAFQEFPQVEACRL 136

Query: 138 FSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLHQTVSESEAERLLTEAEK 197
           +SKFS +   I  IP  + + + S+  GRPG CY D+P+D++  T+ ES       + ++
Sbjct: 137 YSKFSARPSSIQHIPFVIEKAVRSSIYGRPGACYIDVPADLV--TLQESTTS---IKYKE 196

Query: 198 SARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTP 257
                 +     S ++ A S+L++A++PL++ GKGAA + AE  ++KLVE   +PFLPTP
Sbjct: 197 CCMPPPLSLAETSAVRAAASVLRSAKQPLLIIGKGAAYSHAEDSIRKLVELCNLPFLPTP 256

Query: 258 MGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDISE 317
           MGKG++ D H     AARS A+   DV ++ GARLNW+LHFG PP++  DVKFI +DI  
Sbjct: 257 MGKGVVPDNHPNCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQADVKFIQIDICA 316

Query: 318 EEVELR-KPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEAISQKAKENVSKMEVQL 377
           EE+    +P + L+GD   V + + ++   +P+       W E + +K K N +  +   
Sbjct: 317 EELGNNVRPSVTLLGDVNAVSKQLLEQFVKNPWQYPTDSKWWETLREKRKNNEAVSKELA 376

Query: 378 ARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWG 437
           ++  +P N+ T    +++ +         +VSEGANTMD+GR+VL    PR RLDAG++G
Sbjct: 377 SKKSLPMNYYTVFYHVQEQLPRN----CFIVSEGANTMDIGRTVLQNYLPRHRLDAGSFG 436

Query: 438 TMGVGLGYCIAAAVA----SPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGG 497
           TMGVGLG+ IAAAV     SP + V+ VEGDS FGFS MEVET+ RY LP++++V NN G
Sbjct: 437 TMGVGLGFAIAAAVVAKERSPGQRVICVEGDSAFGFSGMEVETICRYNLPIIILVVNNNG 496

Query: 498 VYGG-DRRTPQEI---TGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALA 557
           +Y G D  T  +I    G     P P   +P+++Y +++ AFGGKGY+V+TPEEL+ +L 
Sbjct: 497 IYQGFDADTWGKILNFQGTATTIP-PMCLLPNSHYEQVMTAFGGKGYFVQTPEELQDSLR 556

Query: 558 ESF-SARKPAVVNVKIDP 566
           ++     KP ++N+ I+P
Sbjct: 557 QALKDTSKPCLINIMIEP 564

BLAST of MC08g1837 vs. NCBI nr
Match: XP_022158505.1 (2-hydroxyacyl-CoA lyase [Momordica charantia])

HSP 1 Score: 1145 bits (2962), Expect = 0.0
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG
Sbjct: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578

BLAST of MC08g1837 vs. NCBI nr
Match: XP_023524913.1 (2-hydroxyacyl-CoA lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1088 bits (2813), Expect = 0.0
Identity = 543/578 (93.94%), Postives = 563/578 (97.40%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAGD++ DLKFHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQIEAVKPFSKFSVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QTVSESEAERLL  AE+ + RE IHRVPNSQI+EAISLLK+AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVESTGIPFLPTPMGKGLL DTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFILVDI +EEVELRKP LGLIGDAKQ+LESINKEIKDDPF LGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKA++NV+KMEVQLARDVVPFNF+TPMRIIRDAISA GSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSAL+ESFSAR PAV+NVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 578

BLAST of MC08g1837 vs. NCBI nr
Match: KAG7036762.1 (2-hydroxyacyl-CoA lyase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1086 bits (2809), Expect = 0.0
Identity = 542/578 (93.77%), Postives = 562/578 (97.23%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAGD++ DLKFHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVRADLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQIEAVKPFSKFSVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QTV+ESEAERLL  AE+ + RE IHRVPNSQI+EAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVESTGIPFLPTPMGKGLL DTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFIL+DI +EEVELRKP LGLIGDAKQ+LESINKEIKDDPF LGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILIDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKA++NV+KMEVQLARDVVPFNF+TPMRIIRDAISA GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSAL+ESFSAR PAV+NVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of MC08g1837 vs. NCBI nr
Match: KAG6607061.1 (2-hydroxyacyl-CoA lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1085 bits (2806), Expect = 0.0
Identity = 542/578 (93.77%), Postives = 561/578 (97.06%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAGD++ DLKFHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVRADLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQIEAVKPFSKFSVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QTV+ESEAERLL  AE+ + RE IHRVPNSQI+EAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVESTGIPFLPTPMGKGLL DTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFIL+DI  EEVELRKP LGLIGDAKQ+LESINKEIKDDPF LGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILIDICMEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKA++NV+KMEVQLARDVVPFNF+TPMRIIRDAISA GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSAL+ESFSAR PAV+NVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of MC08g1837 vs. NCBI nr
Match: XP_038893948.1 (2-hydroxyacyl-CoA lyase [Benincasa hispida])

HSP 1 Score: 1085 bits (2805), Expect = 0.0
Identity = 544/578 (94.12%), Postives = 559/578 (96.71%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDS+ DLQNDLKFHGPIDGNV AAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSSEDLQNDLKFHGPIDGNVFAAMALARYGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTG PGVLLTVSGPGCVHGLAGVSNAM NAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGSPGVLLTVSGPGCVHGLAGVSNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQ+EAVKPFSK SVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QTVSESEAERLL  AE+   RE IHRVPNSQI+EAISLLK+AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVSESEAERLLAAAEEFTHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVESTGIPFLPTPMGKGLL DTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFILVDI +EEVELRKP LGLIGDAK+VLESINKEIKDDPF LGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKAK+NV+KMEVQLARDVVPFNFLTPMRIIRDAISA GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLARDVVPFNFLTPMRIIRDAISALGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMG+GLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGIGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSA+YHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSALAESFSARKPAVVNVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of MC08g1837 vs. ExPASy TrEMBL
Match: A0A6J1DW15 (2-hydroxyacyl-CoA lyase OS=Momordica charantia OX=3673 GN=LOC111024979 PE=3 SV=1)

HSP 1 Score: 1145 bits (2962), Expect = 0.0
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG
Sbjct: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578

BLAST of MC08g1837 vs. ExPASy TrEMBL
Match: A0A6J1KDD5 (2-hydroxyacyl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111492838 PE=3 SV=1)

HSP 1 Score: 1084 bits (2803), Expect = 0.0
Identity = 539/578 (93.25%), Postives = 562/578 (97.23%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAGD++ DLKFHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQIEAVKPFSKFSVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QTV+E+EAERLL  AE+ + RE IHRVPNSQI+EAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTETEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVESTGIPFLPTPMGKGLL DTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFILVDI +EEVE+RKP LGLIGDAKQ+LESINKEIKDDPF LGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVEMRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKA++NV+KMEVQLARDVVPFNF+TPMRIIRDAI+A GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAITALGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPV+VIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVIVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSAL+ESFSAR PAV+NVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of MC08g1837 vs. ExPASy TrEMBL
Match: A0A6J1GAR5 (2-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111452318 PE=3 SV=1)

HSP 1 Score: 1082 bits (2799), Expect = 0.0
Identity = 540/578 (93.43%), Postives = 561/578 (97.06%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAGD++ DLKFHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQIEAVKPFSKFSVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QTV+ESEAE LL  AE+ + RE IHRVPNSQI+EAISLLK+AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAEGLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           P+KK VESTGIPFLPTPMGKGLL DTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PMKKFVESTGIPFLPTPMGKGLLPDTHKLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFILVDI +EEVELRKP LGLIGDAKQ+LESINKEIKDDPF LGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKA++NV+KMEVQLARDVVPFNF+TPMRIIRDAISA GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSAL+ESFSAR PAV+NVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of MC08g1837 vs. ExPASy TrEMBL
Match: A0A5A7VDZ6 (2-hydroxyacyl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2030G00330 PE=3 SV=1)

HSP 1 Score: 1071 bits (2770), Expect = 0.0
Identity = 537/578 (92.91%), Postives = 557/578 (96.37%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSA  LQNDL+FHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQ+EAVKPFSK SVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QT+SESEAERLL  AE+ A RE I RVPNSQI+EAISLLK+AERPLIVFGKGAAIARAEG
Sbjct: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVE+TGIPFLPTPMGKGLL DTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFILVDI +EEVELRKP LGLIGDAK+VLESINKEIKDDPF LGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKAK+NV+KMEVQLA+DVVPFNFLTPMRIIRDAI A GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+Y KMIEAFGGKGYYVE+P
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSALAESFSARKPAVVNVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of MC08g1837 vs. ExPASy TrEMBL
Match: A0A1S3B9H9 (2-hydroxyacyl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103487508 PE=3 SV=1)

HSP 1 Score: 1071 bits (2770), Expect = 0.0
Identity = 537/578 (92.91%), Postives = 557/578 (96.37%), Query Frame = 0

Query: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSA  LQNDL+FHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQ+EAVKPFSK SVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240
           QT+SESEAERLL  AE+ A RE I RVPNSQI+EAISLLK+AERPLIVFGKGAAIARAEG
Sbjct: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVE+TGIPFLPTPMGKGLL DTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360
           PPKWSKDVKFILVDI +EEVELRKP LGLIGDAK+VLESINKEIKDDPF LGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360

Query: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420
           ISQKAK+NV+KMEVQLA+DVVPFNFLTPMRIIRDAI A GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480

Query: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540
           LPVVVIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+Y KMIEAFGGKGYYVE+P
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578
           EELKSALAESFSARKPAVVNVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of MC08g1837 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 915.6 bits (2365), Expect = 2.0e-266
Identity = 452/561 (80.57%), Postives = 501/561 (89.30%), Query Frame = 0

Query: 18  IDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 77
           IDGNVL A +L+  GV  MFGVVGIPVTSLA+RA++LGIRFIAFHNEQSAGYAASAYGYL
Sbjct: 12  IDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMALGIRFIAFHNEQSAGYAASAYGYL 71

Query: 78  TGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRDFGRGDFQELDQIEAVKP 137
           TG+PG+LLTVSGPGCVHGLAG+SNA  N WPMVMISGSCDQRD GRGDFQELDQIEAVK 
Sbjct: 72  TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQIEAVKA 131

Query: 138 FSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLHQTVSESEAERLLTEAEK 197
           FSK S KAKD+ EIP+CV++VL  A SGRPGGCY D+P+DVL Q +SESEA++L+ E E+
Sbjct: 132 FSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKLVDEVER 191

Query: 198 SARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTP 257
           S + E I     S+I+ A+SLL+ AERPLIVFGKGAA +RAE  LKKLVE TGIPFLPTP
Sbjct: 192 SRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLPTP 251

Query: 258 MGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDISE 317
           MGKGLL DTHE +ATAARSLAIGKCDVALVVGARLNWLLHFGE PKW KDVKFILVD+SE
Sbjct: 252 MGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVKFILVDVSE 311

Query: 318 EEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEAISQKAKENVSKMEVQLA 377
           EE+ELRKP LG++GDAK V+  +N+EIKDDPF LGKS+ WVE+IS+KAKEN  KME+QLA
Sbjct: 312 EEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEKMEIQLA 371

Query: 378 RDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGT 437
           +DVVPFNFLTPMRIIRDAI A   P+P++VSEGANTMDVGRSVLVQ EPRTRLDAGTWGT
Sbjct: 372 KDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGT 431

Query: 438 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYGGD 497
           MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY L VV+IVFNNGGVYGGD
Sbjct: 432 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNGGVYGGD 491

Query: 498 RRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALAESFSARKPA 557
           RR P+EI+GP+K DPAPTSFVP+A YHK+IEAFGGKGY VETP+ELKSALAESF+ARKPA
Sbjct: 492 RRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESFAARKPA 551

Query: 558 VVNVKIDPFAGAESGRLQHKN 579
           VVNV IDPFAGAESGRLQHKN
Sbjct: 552 VVNVIIDPFAGAESGRLQHKN 572

BLAST of MC08g1837 vs. TAIR 10
Match: AT3G48560.1 (chlorsulfuron/imidazolinone resistant 1 )

HSP 1 Score: 168.3 bits (425), Expect = 1.8e-41
Identity = 135/493 (27.38%), Postives = 227/493 (46.04%), Query Frame = 0

Query: 17  PIDGNVLAAMALARCGVDRMF---GVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASA 76
           P  G  +   AL R GV+ +F   G   + +    TR+ S  IR +   +EQ   +AA  
Sbjct: 96  PRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSS--IRNVLPRHEQGGVFAAEG 155

Query: 77  YGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRDFGRGDFQELDQIE 136
           Y   +G+PG+ +  SGPG  + ++G+++A+ ++ P+V I+G   +R  G   FQE   +E
Sbjct: 156 YARSSGKPGICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPIVE 215

Query: 137 AVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLHQTV--SESEAERL 196
             +  +K +    D+ +IP  + +    A SGRPG    D+P D+  Q    +  +A RL
Sbjct: 216 VTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRL 275

Query: 197 LTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVESTGI 256
                +  +  E     +S +++ + L+  +++P++  G G   +  E  L + VE TGI
Sbjct: 276 PGYMSRMPKPPE-----DSHLEQIVRLISESKKPVLYVGGGCLNSSDE--LGRFVELTGI 335

Query: 257 PFLPTPMGKGLLSDTHELA-------ATAARSLAIGKCDVALVVGARLNWLLHFGEPPKW 316
           P   T MG G      EL+        T   + A+   D+ L  G R +  +  G+   +
Sbjct: 336 PVASTLMGLGSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRV-TGKLEAF 395

Query: 317 SKDVKFILVDISEEEVELRK-PRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEAISQ 376
           +   K + +DI   E+   K P + + GD K  L+ +NK +++             A   
Sbjct: 396 ASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLEN------------RAEEL 455

Query: 377 KAKENVSKMEVQLARDVVPFNFLT------PMRIIRDAISAQGSPAPILVSEGANTMDVG 436
           K    V + E+ + +   P +F T      P   I+         A I    G + M   
Sbjct: 456 KLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAA 515

Query: 437 RSVLVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLV 491
           +    + +PR  L +G  G MG GL   I A+VA+PD +VV ++GD  F  +  E+ T+ 
Sbjct: 516 QFYNYK-KPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIR 565

BLAST of MC08g1837 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.5e-08
Identity = 119/574 (20.73%), Postives = 216/574 (37.63%), Query Frame = 0

Query: 25  AMALARCGVDRMFGVVG-IPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLTGRPG- 84
           A  L + GV  +F V G   +T L        +  I   NE +AGYAA  Y    G    
Sbjct: 52  ARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYARSRGVGAC 111

Query: 85  -VLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRDFGRG----------DFQELDQ 144
            V  TV G   ++ +AG   A +   P++ I G  +  D+G            DF +  +
Sbjct: 112 VVTFTVGGLSVLNAIAG---AYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQ--E 171

Query: 145 IEAVKPFSKFSVKAKDISEIPNCVAQVLSSA-ASGRPGGCYFDLPSD---VLHQTVSESE 204
           +   +  + +     ++ +    + + +S+A    +P   Y  +  +   + H T S   
Sbjct: 172 LRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKP--VYISVSCNLAAIPHHTFSRDP 231

Query: 205 AERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEGPLKKLVE 264
               L     +    E      + ++  +  L  A +P++V G    +A+A     +L +
Sbjct: 232 VPFSLAPRLSNKMGLE------AAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELAD 291

Query: 265 STGIPFLPTPMGKGLLSDTHE--------LAATAARSLAIGKCDVALVVGARLNWLLHFG 324
           ++G      P  KG + + H           +T   S  +   D  +  G   N     G
Sbjct: 292 ASGYALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVG 351

Query: 325 EPPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVE 384
                 K+ K I+V      V    P  G I         ++K +K              
Sbjct: 352 YSLLLKKE-KAIVVQPDRITV-ANGPTFGCI-LMSDFFRELSKRVKR------------- 411

Query: 385 AISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRS 444
             ++ A EN  ++ V   +  +      P+R+       Q     +L SE A   + G S
Sbjct: 412 --NETAYENYHRIFVPEGKP-LKCESREPLRVNTMFQHIQ----KMLSSETAVIAETGDS 471

Query: 445 VLVQTEPRTRLDAG-----TWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVE 504
                + +     G      +G++G  +G  +  A ASP++ V+A  GD  F  +  ++ 
Sbjct: 472 WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQVTVQDIS 531

Query: 505 TLVRYQLPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAF-GGK 564
           T++R     ++ + NNGG          EI     HD  P + + + NY  +++A   G+
Sbjct: 532 TMLRNGQKTIIFLINNGGY-----TIEVEI-----HD-GPYNVIKNWNYTGLVDAIHNGE 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LF462.8e-26580.572-hydroxyacyl-CoA lyase OS=Arabidopsis thaliana OX=3702 GN=HACL PE=1 SV=1[more]
Q0JMH02.5e-25074.692-hydroxyacyl-CoA lyase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0505400... [more]
P0AFI11.2e-12743.34Oxalyl-CoA decarboxylase OS=Escherichia coli O157:H7 OX=83334 GN=oxc PE=3 SV=1[more]
P0AFI01.2e-12743.34Oxalyl-CoA decarboxylase OS=Escherichia coli (strain K12) OX=83333 GN=oxc PE=1 S... [more]
Q8CHM75.7e-12543.372-hydroxyacyl-CoA lyase 1 OS=Rattus norvegicus OX=10116 GN=Hacl1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022158505.10.0100.002-hydroxyacyl-CoA lyase [Momordica charantia][more]
XP_023524913.10.093.942-hydroxyacyl-CoA lyase-like [Cucurbita pepo subsp. pepo][more]
KAG7036762.10.093.772-hydroxyacyl-CoA lyase [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6607061.10.093.772-hydroxyacyl-CoA lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038893948.10.094.122-hydroxyacyl-CoA lyase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1DW150.0100.002-hydroxyacyl-CoA lyase OS=Momordica charantia OX=3673 GN=LOC111024979 PE=3 SV=1[more]
A0A6J1KDD50.093.252-hydroxyacyl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111492838 PE=3 SV=1[more]
A0A6J1GAR50.093.432-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111452318 PE=3 ... [more]
A0A5A7VDZ60.092.912-hydroxyacyl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B9H90.092.912-hydroxyacyl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103487508 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G17380.12.0e-26680.57Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT3G48560.11.8e-4127.38chlorsulfuron/imidazolinone resistant 1 [more]
AT4G33070.11.5e-0820.73Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 25..185
e-value: 3.8E-40
score: 137.3
NoneNo IPR availableGENE3D3.40.50.970coord: 364..578
e-value: 1.0E-64
score: 219.5
NoneNo IPR availableGENE3D3.40.50.970coord: 18..199
e-value: 1.7E-43
score: 150.1
NoneNo IPR availableGENE3D3.40.50.1220coord: 208..362
e-value: 1.8E-37
score: 130.5
NoneNo IPR availablePANTHERPTHR43710:SF22-HYDROXYACYL-COA LYASE 1coord: 18..578
NoneNo IPR availableCDDcd07035TPP_PYR_POX_likecoord: 27..175
e-value: 4.86485E-46
score: 157.308
NoneNo IPR availableCDDcd02004TPP_BZL_OCoD_HPCLcoord: 385..564
e-value: 3.3538E-83
score: 255.535
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 212..339
e-value: 3.3E-32
score: 111.1
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 412..561
e-value: 8.2E-29
score: 100.4
IPR045025TPP-binding domain containing protein HACL1-likePANTHERPTHR437102-HYDROXYACYL-COA LYASEcoord: 18..578
IPR000399TPP-binding enzyme, conserved sitePROSITEPS00187TPP_ENZYMEScoord: 446..465
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 209..366
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 20..187
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 392..567

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1837.1MC08g1837.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001561 fatty acid alpha-oxidation
cellular_component GO:0005777 peroxisome
molecular_function GO:0016829 lyase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0003824 catalytic activity