MC08g1777 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1777
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionpfkB-like carbohydrate kinase family protein
LocationMC08: 25859949 .. 25866036 (+)
RNA-Seq ExpressionMC08g1777
SyntenyMC08g1777
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGCTTTATTCTGCCGTCCAAAAAGTTAATGGCACTTGGCAAACGATAAACTACGCGAATTTCCCCATCGCTTTTTCCGCTAAACACTCCCACTTTTCCAACGCCATTGCCTCTCTGCAACTCCTTCTTCCTCACTCTGAAGCTCATTTTCACCAATTCTTCAGTTTTCCCACTGACCCATTTCGTGTCCAGCTTCTTCTGGACCCCCCAATTGCTCTCTTGAACCCCTTAACCGGGAATTATTGCGTTTGAAGTTTCACTGATTCAGTGTTTTACTTCTTAGTGTTAGTTCTCACGGTGGGTCGAGGTTGTTTTGGTCTGGTTTGGGTCGATGATGGCCCTTCATTCAGCAGCATTCTGCTTCCATGGCGTTCCTAATGTTACTCATGAATTGTTCACAAACAGGCAATGGGCGGTGAAATCGTCATTTGGGTCTTTGAGAACTCGTGTAGTTTCAGAATCTCGGTTGAGGGTGCAAGGTAGACAGACTCCTCTGTAATTGAGTGTTGAATTTGATCTGTAATGACTTCTCTGTTTTTGTCCTTTTTAGATGATGTATCAAACTAAGCTTGTGTCTAGTGTTAGTTTTTGTTTAGTAAATTAGTGAGAATTTAGTATGTTATTGGAGCAGAAATTTTATGTTTAAATCCTGTTTTAGTTATTTTTATATGGGAATTTTCTTCGTGTTCATTTGGCCCATGTGATAGAAGTGAGTTCGCAGGTAAAAGATATGGGAGGAGTGTTAAGAATTGGTGTAATGGAGAAAATGAGATTGAAGTTCGTTTTTGTAACTTTGAAGATAAAAAACCAAGTGAAATTTGATCAAACATCTACCAATTATTTTCTCTCCATTTAACATTATTTTCAATTTATTAGATTTTTCTGCTAATTTATGAGCTCACAAATGTGGAAAGTCTTTGAGTCTTGAGAATATGTTAAAGAGATTAAATTTTTAATAACCTATCCGCTTACGTTTGTTAATTTGGTGACAATTTATCATTGTGTGAGCTGAAACCTTCTATTTCTTACTCTCAGTGAAAGCGTTTTCTGGAGATGGTGTTTTTGCGGAGTCAAATGATTCATCTCTTGTAGTTTGTTTTGGAGAAATGTTGATTGATTTTGTCCCTACTACTAGTGGACTATCTTTAGCTGAGGCACCTGCATTCAAAAAGGCTCCAGGTGGTGCTCCTGCTAATGTTGCTGTTGGCATATCTCGTCTTGGTGGCTCATCTGCTTTTATAGGCAAGGTAATATAATAAATGTTGCTATGGGTTTTTTATCTGCAATAAATATATACAAACCGTTAAATTATAATCTGTGGTTTATTAAAATAAATATTCTCAATGGTGTTTAGACGAGTACACCTCGATCTTATGGGACAACTGGTTAGCCATGCAAGTACAACATTTGTTTGCCAAAGAAATGGCTTGAACCCATTCCTCTAACCTAGTCAGTGGTTATTATTCAGACCTCTTTCATTGGTGAAAACTTGTTTACTTCTGTGTAAATAGAAGGTACACTTATTTTTGGAGGAGGAATCAAGCCTTTTAACATTTTGCTTCCACTAATTTATTTGGTCAGATCTTAGTTTTTCATATTGAATCACACTTGTTGCCATATGGTACTTCTACAGTTAGAGAAGATAATGAAATAATGAAAGGAAGATACTTTTTCCCATGGAGAGTTTCTTAAGGTTTTCTTAACAACATTCACAGGTTGGTGCAGATGAATTTGGTTACATGCTTGCTGATATATTGAAGGAAAACAATGTGAGAAATGAAGGGATGCTGTTTGATCCTGGGGCACGAACGGCTTTAGCATTTGTTACTCTGCGAAATGATGGGGAACGCGAGTTCATGTTTTACCGCAACCCTAGTGCTGACATGTTACTTCAAGAATCTGAACTCAATTATGATTTGATCAGGAAGGTAAGAATGATTTACGAAGTAAATTTTAGATAGCAAATAAGAAAATGCAATAGTTTGCAACGATTATAGAAGAGTTTGTTTTATTATTTTATCCGTAAGTGTCCAGACTAGCATGTGCTTGTGCACATCTATATTAACCTTACTAGACAACCGTGTGACTCTAAAACATAATATATTAGGTTATAGACAAAATTTGTAGGATATTAATCTTAGGTAAGTGGTTGCCATACTTTGATTTGAACTATTTTCTCTAAGCTCTTTACTTTTTTCGCTTGCCTTGATGACCTTTAGGCCAATCCATGTTGGTTGATCATATAAGAGTAATTATAGTTTATTTAAAATTCAAAGTTCAATATCTTAATCTACCACTAAATCCAAAAATTTAAGAAGAGGGTTAGAATAAGGTCATTTTCTCTCACTCCTTTGGGAGTTTGTATCCTTGAACTTTTTCTCCTTTTCATATATCAATGAAAAGTTATTTCTTGTTCTAGAAAAATACAAGTCATTTTGCTCTATACTATGTCAAACTACTAAATCTAAAAATTTAAGCCGATAGATTATCCATATTTAATCTTTTATTCCTCCCTTGGGGGGGTTGTCACTGAGCTTATATTTGATCTTATTTCACAGGCGAAGATCTTTCATTATGGTTCTATAAGTCTTATTACTGAACCATGCAAGTCGGCTCACATTGCTGCAGCAAAAGCTGCAAAAGATGCTGGTGCAGTTCTTTCCTATGATCCTAACCTGAGGCTTCCACTGTGGCCTTCTGCAGACAGTGCCATAGAAGGAATTCTAAGCATATGGGATATCGCAGATATTATTAAGGTAGTTTTCATTTGTTCCTTTTTTGCCATGAAATCATCCGGATAACCTATTAATGAAATGTAGATATGTAGGCATAGCCTGTCTGGTCAATCTGAATACATAAATCAAATATCAATTATCAAATTTAAAAAAGAAAATTAAGGGAAAGCTGACATATGTACTGTAATGTACATTTGCAGATAAGTGAAGAAGAGATTAAATTTCTGACAAGAGGAGAAGATCCATTTGATGATGCTGTTGTTCGTAAACTGTTTCATCCAAATCTTAAATTACTTCTGGTCACTGAAGGTCCTGGTGGTTGTAGATATTATACCAAGGTAAGAATCTCAAACTATGTATTCCAAAAAAAAAAAAAAAAATAATGATAATAAAAAAACCAAGGACTAAATAGCCAAGCTTAAAAATATGTTCTGATGTTGCACGTCATACTGTTTTTGTCATGGCAGTTTAATTTGGTTATTGACTACCAAAACTGATATTTATTGAAGGCCTTTAGGTAGAGTTTATAGAAAATATATGGGGTGGATTCGAAGTAGACAAGATCCTACCAATATGGAGAGATATGAAGTCGGACCTGTTTTTCTAACAAGTGAGTACAAGAGTCATGTCCAAGACTTAGCCATGTAAGATAAAATCCTCCTATACCAAATAAAAGACTCCTATACCAAATAAAAGATTGGTGAACCTCAGGGGACAAAGTCACGTCATCAATATCCTTAGTATCCTAATCATATTTCTAACACTTATCCTAATGCTATCTCTAACAACCTTTTTCTAGGGGAAGATTGTTAGGATATTGCCAAAATCGCACATTAGTTGGGACTAGGAATGAGGCAAACCATTGGTTTATTTAAAAGTATACTATCCTCATTAACATGAGACATTTTGGATAAGGTACAAATGTAAATCTAAGAGGACTAGTTCAAAGCGAACAATGTGTGGGAATGAGGAGCTAAACCTGTTGTCCTAAATTTATCATCATGACATATTTAAATATTAAACATCTTGAGATTCAAAATGAGATGGCTCCTCACCACTGCACATTAGCAGAAAAAAGTGAGTGAATACCCAGTCCCATGCAAGTTTCTCAATCGGTTTCCTTTATCCTGTTGCAGAAATTTGCCATATACAGTCTGCCTTTACACTATTCTTGACACCTACTAATTATTTCATTACTTTAATATCTAATCATCGAAGTAGTTAGTAATTGATCATATTTGGGTCTAGATCTCATCCAAATTACATGCATAAGATTTTACCAAGAACTCTTTAGATTACCAATCTAAACTCAATCCTTTAATATGTAACATTTTTATATATTTGAAAATGTAGATTTGATATCGAGGATTGTGATGGATCCCAAACAACTAGAAATATATTGAAAGATAAAAGATAGAAGAACTACAATAACTACTTAGTAATTCATGGTACTTGGACTCTTCCCTCTCTTGAGTAATCTAACTCAAGCCTAAACTACACTAAACAATTGTCTTCCTCAATCCTTTCCTGCTTACTCTATTTATAACAAATCTCCCTGACAACAACTAATTACTAATATACTCTTAATACCCTTATAATATTCTGAATTCTATCCTAAAAGCATTTCTATCAGATTGTACTAGGAAAGACGAGATTTCAGGCACAGGCCCGAACAAATACTTTAATTGAAAATGCATGTGATGCATGTTATGCAGGAATTCAGTGGAAGAGTGAAGGGTCTAAAGGTGGATGCAGTTGACACCACTGGTGCAGGGGATGCCTTTGTTGCTGGCATATTATCACAGCTAGCCACCGACCTCTCTTTGCTTCAGGTACCTTTTCAGAATTGAAAAACTGTGCATATTTTGAGGAGTACTGAACACCTCTTAATTGCCTTTTTAGTCCTTTAGCGAACTTCCTCCCAATGTTTTAAAAGGCTGAAGGTGCTTTTGGGGCGCCTTCTTTTAACTTGACAAGTGTTAAATTGTCATTAAACTTGAAAAGTGGCTAGTAATGTTAATTCGGAAAGAAATGACAATATAAGAGTTTAGATACGTGGTCTTCTGCTATGATACTAGTGGGGATGTCAAAACTTCATTTTGAAATATGAACTCGAGGATTTATATATATGGTACAACCTCCAGGCTAGATTTGAGGTTCTTTTAAACTATCACTAAACTAAGATAAGCTATATTTTACCTCATTTAGATATTCTTAACAATGAGATGCATAGAGCTGCAATCCTAAGATAAGCTATATTTTACCTCATTTAGATATTCTTAACCATGCAATGCATAGAGCTGCAATCCTGAACTTTAGTATGCAACAAGAGAGAAAATATTGAGAAGATATTCTATTTGTACAAATATGGTATAGCATATAGGTGTGTTTAATCTTTGTTTATTACTCTTTGTATTTTAAACCGTTCTAATATTGATAGAAGTTTTATTTTTCGACTGTAATGTTGTCTCTTGTGTCTATCTATTACTTGAACTTTAAAAAATTTCTGATAGGTCATTAAACTTTTAACTTTATGTCTAATAAATCTCTACATTAACTTGCGTACATAGGATGTTGGGTGTATTATTTAAAAGTTATGTAGCAAGATGATTTTGGCAAATTTAGGCATTTGATGGTGTAGAATAGAATGTTTTTTATGATCCATTTTTTTGTTTCGTTTGAGAGATAAAAATTTACTAGATACAAAAGTGAAAGTTTATAGATCTATTAGAATTTTTTAAAAGTACAGGGAACTATTCATTCAAAAAAATTACAGCCCAAAGGTTCAAAAGGATCAAATTTTAATTTAACCTTATTTATTCAAAAAAATTACTGTGTCTTGTCAGAAGAATGGCAAATGAAATAACAGTTTGTGTCTGTCTTTGAACATTGTAGAAAGAGGACAAATTGAGAGATGCTCTTCTGTTTGCCAATGCTTGTGGTGCATTGACTGTCATGGAGAGAGGGGCCATCCCTGCTTTGCCAAACAAAGAAGCTGTACTTAATGCAATCTTCAAAACTGTGTCCTAAGTCCCTGTTTCTCTCTCTAGGATCTGTATGTATAATACCCAAGACCTAATTTCCACAAATTTTGGGCAGATTTTGTAATTTCTCTCAATTAGGTTTAGCTGTTTGTGAAGTTGGGTTGTTGGTTCTGTTGTTTGGATCTTCCATTGTATTTGAAATGATTATGTCTCATTCATTTATGGTAATGAAACAACATCAATAATATGGCTTCTATTGCTGTAATGTGCTGAGTCTCGTGATGGTTTCTTGCCCGACCATGCCCCTCGGATTTTGATTCTATCTATTGCTGAGTGGACATGGCGAGTTATGTTGTTCTATTTTCTTC

mRNA sequence

AAAGCTTTATTCTGCCGTCCAAAAAGTTAATGGCACTTGGCAAACGATAAACTACGCGAATTTCCCCATCGCTTTTTCCGCTAAACACTCCCACTTTTCCAACGCCATTGCCTCTCTGCAACTCCTTCTTCCTCACTCTGAAGCTCATTTTCACCAATTCTTCAGTTTTCCCACTGACCCATTTCGTGTCCAGCTTCTTCTGGACCCCCCAATTGCTCTCTTGAACCCCTTAACCGGGAATTATTGCGTTTGAAGTTTCACTGATTCAGTGTTTTACTTCTTAGTGTTAGTTCTCACGGTGGGTCGAGGTTGTTTTGGTCTGGTTTGGGTCGATGATGGCCCTTCATTCAGCAGCATTCTGCTTCCATGGCGTTCCTAATGTTACTCATGAATTGTTCACAAACAGGCAATGGGCGGTGAAATCGTCATTTGGGTCTTTGAGAACTCGTGTAGTTTCAGAATCTCGGTTGAGGGTGCAAGTGAAAGCGTTTTCTGGAGATGGTGTTTTTGCGGAGTCAAATGATTCATCTCTTGTAGTTTGTTTTGGAGAAATGTTGATTGATTTTGTCCCTACTACTAGTGGACTATCTTTAGCTGAGGCACCTGCATTCAAAAAGGCTCCAGGTGGTGCTCCTGCTAATGTTGCTGTTGGCATATCTCGTCTTGGTGGCTCATCTGCTTTTATAGGCAAGGTTGGTGCAGATGAATTTGGTTACATGCTTGCTGATATATTGAAGGAAAACAATGTGAGAAATGAAGGGATGCTGTTTGATCCTGGGGCACGAACGGCTTTAGCATTTGTTACTCTGCGAAATGATGGGGAACGCGAGTTCATGTTTTACCGCAACCCTAGTGCTGACATGTTACTTCAAGAATCTGAACTCAATTATGATTTGATCAGGAAGGCGAAGATCTTTCATTATGGTTCTATAAGTCTTATTACTGAACCATGCAAGTCGGCTCACATTGCTGCAGCAAAAGCTGCAAAAGATGCTGGTGCAGTTCTTTCCTATGATCCTAACCTGAGGCTTCCACTGTGGCCTTCTGCAGACAGTGCCATAGAAGGAATTCTAAGCATATGGGATATCGCAGATATTATTAAGATAAGTGAAGAAGAGATTAAATTTCTGACAAGAGGAGAAGATCCATTTGATGATGCTGTTGTTCGTAAACTGTTTCATCCAAATCTTAAATTACTTCTGGTCACTGAAGGTCCTGGTGGTTGTAGATATTATACCAAGGAATTCAGTGGAAGAGTGAAGGGTCTAAAGGTGGATGCAGTTGACACCACTGGTGCAGGGGATGCCTTTGTTGCTGGCATATTATCACAGCTAGCCACCGACCTCTCTTTGCTTCAGAAAGAGGACAAATTGAGAGATGCTCTTCTGTTTGCCAATGCTTGTGGTGCATTGACTGTCATGGAGAGAGGGGCCATCCCTGCTTTGCCAAACAAAGAAGCTGTACTTAATGCAATCTTCAAAACTGTGTCCTAAGTCCCTGTTTCTCTCTCTAGGATCTGTATGTATAATACCCAAGACCTAATTTCCACAAATTTTGGGCAGATTTTGTAATTTCTCTCAATTAGGTTTAGCTGTTTGTGAAGTTGGGTTGTTGGTTCTGTTGTTTGGATCTTCCATTGTATTTGAAATGATTATGTCTCATTCATTTATGGTAATGAAACAACATCAATAATATGGCTTCTATTGCTGTAATGTGCTGAGTCTCGTGATGGTTTCTTGCCCGACCATGCCCCTCGGATTTTGATTCTATCTATTGCTGAGTGGACATGGCGAGTTATGTTGTTCTATTTTCTTC

Coding sequence (CDS)

ATGATGGCCCTTCATTCAGCAGCATTCTGCTTCCATGGCGTTCCTAATGTTACTCATGAATTGTTCACAAACAGGCAATGGGCGGTGAAATCGTCATTTGGGTCTTTGAGAACTCGTGTAGTTTCAGAATCTCGGTTGAGGGTGCAAGTGAAAGCGTTTTCTGGAGATGGTGTTTTTGCGGAGTCAAATGATTCATCTCTTGTAGTTTGTTTTGGAGAAATGTTGATTGATTTTGTCCCTACTACTAGTGGACTATCTTTAGCTGAGGCACCTGCATTCAAAAAGGCTCCAGGTGGTGCTCCTGCTAATGTTGCTGTTGGCATATCTCGTCTTGGTGGCTCATCTGCTTTTATAGGCAAGGTTGGTGCAGATGAATTTGGTTACATGCTTGCTGATATATTGAAGGAAAACAATGTGAGAAATGAAGGGATGCTGTTTGATCCTGGGGCACGAACGGCTTTAGCATTTGTTACTCTGCGAAATGATGGGGAACGCGAGTTCATGTTTTACCGCAACCCTAGTGCTGACATGTTACTTCAAGAATCTGAACTCAATTATGATTTGATCAGGAAGGCGAAGATCTTTCATTATGGTTCTATAAGTCTTATTACTGAACCATGCAAGTCGGCTCACATTGCTGCAGCAAAAGCTGCAAAAGATGCTGGTGCAGTTCTTTCCTATGATCCTAACCTGAGGCTTCCACTGTGGCCTTCTGCAGACAGTGCCATAGAAGGAATTCTAAGCATATGGGATATCGCAGATATTATTAAGATAAGTGAAGAAGAGATTAAATTTCTGACAAGAGGAGAAGATCCATTTGATGATGCTGTTGTTCGTAAACTGTTTCATCCAAATCTTAAATTACTTCTGGTCACTGAAGGTCCTGGTGGTTGTAGATATTATACCAAGGAATTCAGTGGAAGAGTGAAGGGTCTAAAGGTGGATGCAGTTGACACCACTGGTGCAGGGGATGCCTTTGTTGCTGGCATATTATCACAGCTAGCCACCGACCTCTCTTTGCTTCAGAAAGAGGACAAATTGAGAGATGCTCTTCTGTTTGCCAATGCTTGTGGTGCATTGACTGTCATGGAGAGAGGGGCCATCCCTGCTTTGCCAAACAAAGAAGCTGTACTTAATGCAATCTTCAAAACTGTGTCCTAA

Protein sequence

MMALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKVGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADSAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYYTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALTVMERGAIPALPNKEAVLNAIFKTVS
Homology
BLAST of MC08g1777 vs. ExPASy Swiss-Prot
Match: Q9C524 (Probable fructokinase-6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g66430 PE=2 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.1e-159
Identity = 289/382 (75.65%), Postives = 325/382 (85.08%), Query Frame = 0

Query: 6   SAAFCFHG--VPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAESN 65
           +  FCF G    +  H L + R  ++K++  S     +S SR  ++ +A S DG    + 
Sbjct: 7   TTTFCFSGPTFRSTPHSLTSKRPISIKATTSS--PSRLSNSRSNLKGRALSSDG---STQ 66

Query: 66  DSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKVGA 125
           +S  VVCFGEMLIDFVPTTSGLSLA+APAFKKAPGGAPANVAVGI+RLGGSSAFIGKVG 
Sbjct: 67  ESPYVVCFGEMLIDFVPTTSGLSLADAPAFKKAPGGAPANVAVGIARLGGSSAFIGKVGE 126

Query: 126 DEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQESE 185
           DEFGYMLA+ILK+NNV N+GM FDPGARTALAFVTL N+GEREFMFYRNPSADMLL+ESE
Sbjct: 127 DEFGYMLANILKDNNVNNDGMRFDPGARTALAFVTLTNEGEREFMFYRNPSADMLLEESE 186

Query: 186 LNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADSAI 245
           L++DLI+KAKIFHYGSISLITEPCKSAHI+AAKAAK+AG +LSYDPNLRLPLWPSAD+A 
Sbjct: 187 LDFDLIKKAKIFHYGSISLITEPCKSAHISAAKAAKEAGVILSYDPNLRLPLWPSADNAR 246

Query: 246 EGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYYTK 305
           E ILSIW+ ADIIKISEEEI FLT+GEDP+DD VVRKLFHP LKLLLVTEGP GCRYYTK
Sbjct: 247 EEILSIWETADIIKISEEEIVFLTKGEDPYDDNVVRKLFHPKLKLLLVTEGPEGCRYYTK 306

Query: 306 EFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALTVM 365
           +FSGRV GLKVD VDTTGAGDAFVAGILSQLA DLSLLQ E++LR+AL+FANACGALTV 
Sbjct: 307 DFSGRVHGLKVDVVDTTGAGDAFVAGILSQLANDLSLLQDEERLREALMFANACGALTVK 366

Query: 366 ERGAIPALPNKEAVLNAIFKTV 386
            RGAIPALP KEAV  A+ K V
Sbjct: 367 VRGAIPALPTKEAVHEALLKAV 383

BLAST of MC08g1777 vs. ExPASy Swiss-Prot
Match: Q9FLH8 (Probable fructokinase-7 OS=Arabidopsis thaliana OX=3702 GN=At5g51830 PE=1 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.3e-133
Identity = 238/325 (73.23%), Postives = 281/325 (86.46%), Query Frame = 0

Query: 63  NDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKVG 122
           +  +LVVCFGEMLIDFVPT  G+SLAEAPAFKKAPGGAPANVAVG+SRLGGSSAFIGKVG
Sbjct: 19  DSETLVVCFGEMLIDFVPTVGGVSLAEAPAFKKAPGGAPANVAVGVSRLGGSSAFIGKVG 78

Query: 123 ADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQES 182
            DEFG MLADIL+ NNV N GM FD  ARTALAFVTLR DGEREF+F+R+PSADMLL ES
Sbjct: 79  DDEFGRMLADILRLNNVDNSGMRFDHNARTALAFVTLRGDGEREFLFFRHPSADMLLLES 138

Query: 183 ELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADSA 242
           EL+ +LI+KAKIFHYGSISLI EPC+S  + A K AK AG++LSYDPNLRLPLWPS ++A
Sbjct: 139 ELDKNLIQKAKIFHYGSISLIEEPCRSTQLVAMKIAKAAGSLLSYDPNLRLPLWPSEEAA 198

Query: 243 IEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVV-RKLFHPNLKLLLVTEGPGGCRYY 302
            + I+SIW++AD+IKISE+EI FLT G+DP+DD VV +KLFHPNLKLL+V+EGP GCRYY
Sbjct: 199 RKEIMSIWNLADVIKISEDEITFLTGGDDPYDDDVVLQKLFHPNLKLLVVSEGPNGCRYY 258

Query: 303 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 362
           T+EF GRV G+KV  VDTTGAGDAFV+G+L+ LA+DL+LL+ E KLR+ALLFANACGA+T
Sbjct: 259 TQEFKGRVGGVKVKPVDTTGAGDAFVSGLLNSLASDLTLLKDEKKLREALLFANACGAIT 318

Query: 363 VMERGAIPALPNKEAVLNAIFKTVS 387
           V ERGAIPA+P+ +AV + +  T S
Sbjct: 319 VTERGAIPAMPSMDAVQDLLSSTRS 343

BLAST of MC08g1777 vs. ExPASy Swiss-Prot
Match: O82616 (Probable fructokinase-5 OS=Arabidopsis thaliana OX=3702 GN=At4g10260 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.2e-118
Identity = 213/309 (68.93%), Postives = 252/309 (81.55%), Query Frame = 0

Query: 64  DSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKVGA 123
           ++ L+V FGEMLIDFVP TSG+SLAE+  F KAPGGAPANVA  I++LGG SAFIGK G 
Sbjct: 3   NTPLIVSFGEMLIDFVPDTSGVSLAESTGFLKAPGGAPANVACAITKLGGKSAFIGKFGD 62

Query: 124 DEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQESE 183
           DEFG+ML +ILK+N V +EG+ FD  ARTALAFVTL+ DGEREFMFYRNPSADMLL+ESE
Sbjct: 63  DEFGHMLVNILKKNGVNSEGVCFDTNARTALAFVTLKKDGEREFMFYRNPSADMLLKESE 122

Query: 184 LNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADSAI 243
           LN DLI+KAKIFHYGSISLI+EPC++AH+AA K AKDAG +LSYDPN+RLPLWPS ++AI
Sbjct: 123 LNKDLIKKAKIFHYGSISLISEPCRTAHMAAMKTAKDAGVLLSYDPNVRLPLWPSTEAAI 182

Query: 244 EGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYYTK 303
           EGI SIW+ ADIIK+S++E+ FLTRG+   DD VV  L H  LKLL+VT+G  GCRYYTK
Sbjct: 183 EGIKSIWNEADIIKVSDDEVTFLTRGDAEKDD-VVLSLMHDKLKLLIVTDGEKGCRYYTK 242

Query: 304 EFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALTVM 363
           +F GRV G  V AVDTTGAGD+FV   L  L  D S+L  E KL++AL FANACGA+   
Sbjct: 243 KFKGRVPGYAVKAVDTTGAGDSFVGAFLVSLGKDGSILDDEGKLKEALAFANACGAVCTT 302

Query: 364 ERGAIPALP 373
           ++GAIPALP
Sbjct: 303 QKGAIPALP 310

BLAST of MC08g1777 vs. ExPASy Swiss-Prot
Match: Q9M1B9 (Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.0e-117
Identity = 210/318 (66.04%), Postives = 252/318 (79.25%), Query Frame = 0

Query: 60  AESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIG 119
           A + + SL+V FGEMLIDFVPT SG+SLA+AP F KAPGGAPANVA+ ISRLGG +AF+G
Sbjct: 3   ASNGEKSLIVSFGEMLIDFVPTVSGVSLADAPGFIKAPGGAPANVAIAISRLGGRAAFVG 62

Query: 120 KVGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLL 179
           K+G DEFG+MLA ILK+N V  EG+ FD GARTALAFVTLR+DGEREFMFYRNPSADMLL
Sbjct: 63  KLGDDEFGHMLAGILKQNGVSAEGINFDTGARTALAFVTLRSDGEREFMFYRNPSADMLL 122

Query: 180 QESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA 239
           +  ELN D+IR AK+FHYGSISLI EPC+SAH+ A + AK+AGA+LSYDPNLRLPLWPS 
Sbjct: 123 RPDELNLDVIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGALLSYDPNLRLPLWPSK 182

Query: 240 DSAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCR 299
           + A + ILSIWD A++IK+S+EE+ FLT G D  DD     L+H NLKLLLVT G  GCR
Sbjct: 183 EEAQKQILSIWDKAEVIKVSDEELMFLT-GSDKVDDETALSLWHSNLKLLLVTLGEKGCR 242

Query: 300 YYTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGA 359
           YYTK F G V    VDAVDTTGAGD+FV  +L ++  D ++L+ E +LR+ L  ANACGA
Sbjct: 243 YYTKSFRGSVDPFHVDAVDTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRLANACGA 302

Query: 360 LTVMERGAIPALPNKEAV 378
           +T  ++GAIPALP +  V
Sbjct: 303 ITTTKKGAIPALPTESEV 319

BLAST of MC08g1777 vs. ExPASy Swiss-Prot
Match: Q9SID0 (Probable fructokinase-1 OS=Arabidopsis thaliana OX=3702 GN=At2g31390 PE=2 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 3.8e-117
Identity = 210/324 (64.81%), Postives = 255/324 (78.70%), Query Frame = 0

Query: 60  AESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIG 119
           A + D  L+V FGEMLIDFVPT SG+SLAEAP F KAPGGAPANVA+ +SRLGG SAF+G
Sbjct: 2   ASNGDKGLIVSFGEMLIDFVPTESGVSLAEAPGFLKAPGGAPANVAIAVSRLGGRSAFVG 61

Query: 120 KVGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLL 179
           K+G DEFG+MLA IL++N V ++G+ FD GARTALAFVTLR DG+REFMFYRNPSADMLL
Sbjct: 62  KLGDDEFGHMLAGILRKNGVDDQGINFDTGARTALAFVTLRADGDREFMFYRNPSADMLL 121

Query: 180 QESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA 239
           +  ELN DLIR AK+FHYGSISLI EPC+SAH+ A + AK+AGA+LSYDPNLR PLWPS 
Sbjct: 122 RPDELNLDLIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGALLSYDPNLREPLWPSK 181

Query: 240 DSAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCR 299
           + A   I+SIWD A+IIK+S+ E++FLT G +  DD     L+HPNLKLLLVT G  GCR
Sbjct: 182 EEAKTQIMSIWDKAEIIKVSDVELEFLT-GSNKIDDETALTLWHPNLKLLLVTLGEKGCR 241

Query: 300 YYTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGA 359
           YYTK F G V    V+AVDTTGAGD+FV  +L+Q+  D S+L+ E++LR  L FANACGA
Sbjct: 242 YYTKTFKGAVDPFHVNAVDTTGAGDSFVGALLNQIVDDRSVLEDEERLRKVLRFANACGA 301

Query: 360 LTVMERGAIPALPNKEAVLNAIFK 384
           +T  ++GAIPALP+   V + + K
Sbjct: 302 ITTTKKGAIPALPSDAEVRSFLEK 324

BLAST of MC08g1777 vs. NCBI nr
Match: XP_022143431.1 (probable fructokinase-6, chloroplastic [Momordica charantia])

HSP 1 Score: 756 bits (1952), Expect = 3.24e-275
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 0

Query: 1   MMALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFA 60
           MMALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFA
Sbjct: 1   MMALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFA 60

Query: 61  ESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGK 120
           ESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGK
Sbjct: 61  ESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGK 120

Query: 121 VGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQ 180
           VGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQ
Sbjct: 121 VGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQ 180

Query: 181 ESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAD 240
           ESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAD
Sbjct: 181 ESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAD 240

Query: 241 SAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRY 300
           SAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRY
Sbjct: 241 SAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRY 300

Query: 301 YTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGAL 360
           YTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGAL
Sbjct: 301 YTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGAL 360

Query: 361 TVMERGAIPALPNKEAVLNAIFKTVS 386
           TVMERGAIPALPNKEAVLNAIFKTVS
Sbjct: 361 TVMERGAIPALPNKEAVLNAIFKTVS 386

BLAST of MC08g1777 vs. NCBI nr
Match: XP_038890970.1 (probable fructokinase-6, chloroplastic [Benincasa hispida])

HSP 1 Score: 701 bits (1808), Expect = 2.65e-253
Identity = 359/384 (93.49%), Postives = 369/384 (96.09%), Query Frame = 0

Query: 2   MALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAE 61
           MALHS AFCFHGVP VTHE   ++QWAVKSS G  R RVVS+S+L+V+VKAFSGDG   E
Sbjct: 1   MALHSGAFCFHGVPTVTHESLRHKQWAVKSS-GFSRPRVVSKSQLKVKVKAFSGDGGLVE 60

Query: 62  SNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKV 121
           SNDSSLVVCFGEMLIDFVPT SGLSLAEAPAFKKAPGGAPANVAVGI+RLGGSSAFIGKV
Sbjct: 61  SNDSSLVVCFGEMLIDFVPTISGLSLAEAPAFKKAPGGAPANVAVGIARLGGSSAFIGKV 120

Query: 122 GADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 181
           GADEFGYMLADILKENNV NEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE
Sbjct: 121 GADEFGYMLADILKENNVCNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 180

Query: 182 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 241
           SE+NYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS
Sbjct: 181 SEINYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 240

Query: 242 AIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 301
           AIEGILSIWDIADIIKISEEEI FLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY
Sbjct: 241 AIEGILSIWDIADIIKISEEEINFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 300

Query: 302 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 361
           TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLR+ALLFANACGALT
Sbjct: 301 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLREALLFANACGALT 360

Query: 362 VMERGAIPALPNKEAVLNAIFKTV 385
           VMERGAIPALPNK+AVLNAIFKTV
Sbjct: 361 VMERGAIPALPNKDAVLNAIFKTV 383

BLAST of MC08g1777 vs. NCBI nr
Match: XP_023526609.1 (probable fructokinase-6, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526611.1 probable fructokinase-6, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526612.1 probable fructokinase-6, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo] >XP_023526613.1 probable fructokinase-6, chloroplastic isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 698 bits (1801), Expect = 3.09e-252
Identity = 356/385 (92.47%), Postives = 369/385 (95.84%), Query Frame = 0

Query: 2   MALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAE 61
           MALHS AFCFHG P V HE   +RQW VKSS G LR+RVVS+S L+V+VKAFSGDG   E
Sbjct: 1   MALHSGAFCFHGFPTVAHESLRHRQWVVKSS-GFLRSRVVSKSHLKVKVKAFSGDGGLVE 60

Query: 62  SNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKV 121
           SNDSSLVVCFGEMLIDFVPTT+GLSLAEAPAFKKAPGGAPANVAVGI+RLGGSSAFIGKV
Sbjct: 61  SNDSSLVVCFGEMLIDFVPTTNGLSLAEAPAFKKAPGGAPANVAVGIARLGGSSAFIGKV 120

Query: 122 GADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 181
           GADEFGYMLADILKENNV NEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE
Sbjct: 121 GADEFGYMLADILKENNVSNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 180

Query: 182 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 241
           SELNYDLI+KAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA+S
Sbjct: 181 SELNYDLIKKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAES 240

Query: 242 AIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 301
           AIEGILSIWDIADIIKISEEEI FLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY
Sbjct: 241 AIEGILSIWDIADIIKISEEEISFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 300

Query: 302 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 361
           TKEFSGRVKGLKVDA+DTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT
Sbjct: 301 TKEFSGRVKGLKVDAIDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 360

Query: 362 VMERGAIPALPNKEAVLNAIFKTVS 386
           VMERGAIPALP+K+AVL+AIFKTVS
Sbjct: 361 VMERGAIPALPDKDAVLDAIFKTVS 384

BLAST of MC08g1777 vs. NCBI nr
Match: XP_022982732.1 (probable fructokinase-6, chloroplastic [Cucurbita maxima])

HSP 1 Score: 697 bits (1800), Expect = 4.39e-252
Identity = 357/385 (92.73%), Postives = 367/385 (95.32%), Query Frame = 0

Query: 2   MALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAE 61
           MALHS AFCFHG P V HE    RQW VKSS G LR+RVVS+S L+V+VKAFSGDG   E
Sbjct: 1   MALHSGAFCFHGFPTVAHESLRRRQWVVKSS-GFLRSRVVSKSHLKVKVKAFSGDGGLVE 60

Query: 62  SNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKV 121
           SNDSSLVVCFGEMLIDFVPTT+GLSLAEAPAFKKAPGGAPANVAVGI+RLGG SAFIGKV
Sbjct: 61  SNDSSLVVCFGEMLIDFVPTTNGLSLAEAPAFKKAPGGAPANVAVGIARLGGLSAFIGKV 120

Query: 122 GADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 181
           GADEFGYMLADILKENNV NEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE
Sbjct: 121 GADEFGYMLADILKENNVSNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 180

Query: 182 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 241
           SELNYDLI+KAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA+S
Sbjct: 181 SELNYDLIKKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAES 240

Query: 242 AIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 301
           AIEGILSIWDIADIIKISEEEI FLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY
Sbjct: 241 AIEGILSIWDIADIIKISEEEISFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 300

Query: 302 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 361
           TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT
Sbjct: 301 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 360

Query: 362 VMERGAIPALPNKEAVLNAIFKTVS 386
           VMERGAIPALP+K+AVLNAIFKTVS
Sbjct: 361 VMERGAIPALPDKDAVLNAIFKTVS 384

BLAST of MC08g1777 vs. NCBI nr
Match: KAG6581213.1 (putative fructokinase-6, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 697 bits (1800), Expect = 4.39e-252
Identity = 355/385 (92.21%), Postives = 368/385 (95.58%), Query Frame = 0

Query: 2   MALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAE 61
           MALHS AFCFHG P V HE   +RQW VKSS G LR+RVVS+S L+ +VKAFSGDG   E
Sbjct: 1   MALHSGAFCFHGFPTVAHESLRHRQWVVKSS-GFLRSRVVSKSHLKFKVKAFSGDGGLVE 60

Query: 62  SNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKV 121
           SNDSSLVVCFGEMLIDFVPTT+GLSLAEAPAFKKAPGGAPANVAVGI+RLGGSSAFIGKV
Sbjct: 61  SNDSSLVVCFGEMLIDFVPTTNGLSLAEAPAFKKAPGGAPANVAVGIARLGGSSAFIGKV 120

Query: 122 GADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 181
           GADEFGYMLADILKENNV NEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE
Sbjct: 121 GADEFGYMLADILKENNVSNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 180

Query: 182 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 241
           SELNYDLI+KAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA+S
Sbjct: 181 SELNYDLIKKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAES 240

Query: 242 AIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 301
           AIEGILSIWDIADIIK+SEEEI FLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY
Sbjct: 241 AIEGILSIWDIADIIKVSEEEINFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 300

Query: 302 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 361
           TKEFSGRVKGLKVDA+DTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT
Sbjct: 301 TKEFSGRVKGLKVDAIDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 360

Query: 362 VMERGAIPALPNKEAVLNAIFKTVS 386
           VMERGAIPALP+K+AVLNAIFKTVS
Sbjct: 361 VMERGAIPALPDKDAVLNAIFKTVS 384

BLAST of MC08g1777 vs. ExPASy TrEMBL
Match: A0A6J1CQ82 (probable fructokinase-6, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013308 PE=3 SV=1)

HSP 1 Score: 756 bits (1952), Expect = 1.57e-275
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 0

Query: 1   MMALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFA 60
           MMALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFA
Sbjct: 1   MMALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFA 60

Query: 61  ESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGK 120
           ESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGK
Sbjct: 61  ESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGK 120

Query: 121 VGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQ 180
           VGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQ
Sbjct: 121 VGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQ 180

Query: 181 ESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAD 240
           ESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAD
Sbjct: 181 ESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAD 240

Query: 241 SAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRY 300
           SAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRY
Sbjct: 241 SAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRY 300

Query: 301 YTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGAL 360
           YTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGAL
Sbjct: 301 YTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGAL 360

Query: 361 TVMERGAIPALPNKEAVLNAIFKTVS 386
           TVMERGAIPALPNKEAVLNAIFKTVS
Sbjct: 361 TVMERGAIPALPNKEAVLNAIFKTVS 386

BLAST of MC08g1777 vs. ExPASy TrEMBL
Match: A0A6J1IXD2 (probable fructokinase-6, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111481512 PE=3 SV=1)

HSP 1 Score: 697 bits (1800), Expect = 2.12e-252
Identity = 357/385 (92.73%), Postives = 367/385 (95.32%), Query Frame = 0

Query: 2   MALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAE 61
           MALHS AFCFHG P V HE    RQW VKSS G LR+RVVS+S L+V+VKAFSGDG   E
Sbjct: 1   MALHSGAFCFHGFPTVAHESLRRRQWVVKSS-GFLRSRVVSKSHLKVKVKAFSGDGGLVE 60

Query: 62  SNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKV 121
           SNDSSLVVCFGEMLIDFVPTT+GLSLAEAPAFKKAPGGAPANVAVGI+RLGG SAFIGKV
Sbjct: 61  SNDSSLVVCFGEMLIDFVPTTNGLSLAEAPAFKKAPGGAPANVAVGIARLGGLSAFIGKV 120

Query: 122 GADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 181
           GADEFGYMLADILKENNV NEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE
Sbjct: 121 GADEFGYMLADILKENNVSNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 180

Query: 182 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 241
           SELNYDLI+KAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA+S
Sbjct: 181 SELNYDLIKKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAES 240

Query: 242 AIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 301
           AIEGILSIWDIADIIKISEEEI FLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY
Sbjct: 241 AIEGILSIWDIADIIKISEEEISFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 300

Query: 302 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 361
           TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT
Sbjct: 301 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 360

Query: 362 VMERGAIPALPNKEAVLNAIFKTVS 386
           VMERGAIPALP+K+AVLNAIFKTVS
Sbjct: 361 VMERGAIPALPDKDAVLNAIFKTVS 384

BLAST of MC08g1777 vs. ExPASy TrEMBL
Match: A0A6J1FA53 (probable fructokinase-6, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442220 PE=3 SV=1)

HSP 1 Score: 694 bits (1790), Expect = 7.09e-251
Identity = 354/385 (91.95%), Postives = 366/385 (95.06%), Query Frame = 0

Query: 2   MALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAE 61
           MALHS AFCFHG P V HE   +RQW VKSS G LR+RVVS+S L+ +VKAFSGDG   E
Sbjct: 1   MALHSGAFCFHGFPTVAHESLRHRQWVVKSS-GFLRSRVVSKSHLKFKVKAFSGDGGLVE 60

Query: 62  SNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKV 121
           SNDS LVVCFGEMLIDFVPTT+GLSLAEAPAFKKAPGGAPANVAVGI+RLGGSSAFIGKV
Sbjct: 61  SNDSPLVVCFGEMLIDFVPTTNGLSLAEAPAFKKAPGGAPANVAVGIARLGGSSAFIGKV 120

Query: 122 GADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 181
           GADEFGYMLADILKENNV  EGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE
Sbjct: 121 GADEFGYMLADILKENNVSGEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 180

Query: 182 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 241
           SELNYDLI+KAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA+S
Sbjct: 181 SELNYDLIKKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSAES 240

Query: 242 AIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 301
           AIEGILSIWDIADIIKISEEEI FLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY
Sbjct: 241 AIEGILSIWDIADIIKISEEEISFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 300

Query: 302 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 361
           TKEFSGRVKGLKVDA+DTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT
Sbjct: 301 TKEFSGRVKGLKVDAIDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 360

Query: 362 VMERGAIPALPNKEAVLNAIFKTVS 386
           VMERGAIPALP+K+AVLNAIFKTVS
Sbjct: 361 VMERGAIPALPDKDAVLNAIFKTVS 384

BLAST of MC08g1777 vs. ExPASy TrEMBL
Match: A0A1S3C5X9 (probable fructokinase-6, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103496836 PE=3 SV=1)

HSP 1 Score: 686 bits (1771), Expect = 5.57e-248
Identity = 352/385 (91.43%), Postives = 367/385 (95.32%), Query Frame = 0

Query: 2   MALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAE 61
           MAL+S AFCFHGVP VT E   +++WAVKS +G  R+RVVS+S+L+V+VKAFSGDG   E
Sbjct: 1   MALYSGAFCFHGVPTVTLESLRHKEWAVKS-YGLSRSRVVSKSQLKVKVKAFSGDGGSVE 60

Query: 62  SNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKV 121
           SNDSSLVVCFGEMLIDFVPT SGLSLAEAPAFKKAPGGAPANVAVGI+RLGGSSAFIGKV
Sbjct: 61  SNDSSLVVCFGEMLIDFVPTISGLSLAEAPAFKKAPGGAPANVAVGIARLGGSSAFIGKV 120

Query: 122 GADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 181
           GADEFGYMLADILKENNV NEGMLFDPGARTALAFVTLR+DGEREFMFYRNPSADMLLQE
Sbjct: 121 GADEFGYMLADILKENNVCNEGMLFDPGARTALAFVTLRSDGEREFMFYRNPSADMLLQE 180

Query: 182 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 241
           SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAK AKDAGAVLSYDPNLRLPLWPSADS
Sbjct: 181 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKTAKDAGAVLSYDPNLRLPLWPSADS 240

Query: 242 AIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 301
           AIEGILSIWD ADIIKISEEEI FLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY
Sbjct: 241 AIEGILSIWDTADIIKISEEEISFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 300

Query: 302 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 361
           TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLL+KEDKLRDALLFANACGALT
Sbjct: 301 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLEKEDKLRDALLFANACGALT 360

Query: 362 VMERGAIPALPNKEAVLNAIFKTVS 386
           V ERGAIPALPNK+AVLNAIFKTV+
Sbjct: 361 VKERGAIPALPNKDAVLNAIFKTVA 384

BLAST of MC08g1777 vs. ExPASy TrEMBL
Match: A0A0A0L7Q6 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G345390 PE=3 SV=1)

HSP 1 Score: 682 bits (1761), Expect = 1.86e-246
Identity = 351/385 (91.17%), Postives = 366/385 (95.06%), Query Frame = 0

Query: 2   MALHSAAFCFHGVPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAE 61
           MAL+S AFCFHGVP VT E   ++QWAV+S +G  R+RVVS+S+L+V+VKAFSGDG   E
Sbjct: 1   MALYSGAFCFHGVPTVTLESLRHKQWAVRS-YGISRSRVVSKSQLKVKVKAFSGDGGSVE 60

Query: 62  SNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKV 121
           SNDSSLVVCFGEMLIDFVPT SGLSLAEAPAFKKAPGGAPANVAVGI+RLGGSSAFIGKV
Sbjct: 61  SNDSSLVVCFGEMLIDFVPTISGLSLAEAPAFKKAPGGAPANVAVGIARLGGSSAFIGKV 120

Query: 122 GADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQE 181
           GADEFGYMLADILKENNV NEGMLFDPGARTALAFVTLR+DGEREFMFYRNPSADMLLQE
Sbjct: 121 GADEFGYMLADILKENNVCNEGMLFDPGARTALAFVTLRSDGEREFMFYRNPSADMLLQE 180

Query: 182 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 241
           SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS
Sbjct: 181 SELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADS 240

Query: 242 AIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 301
           AIEGILSIW+ ADIIKISEEEI FLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY
Sbjct: 241 AIEGILSIWETADIIKISEEEISFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYY 300

Query: 302 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 361
           TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT
Sbjct: 301 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 360

Query: 362 VMERGAIPALPNKEAVLNAIFKTVS 386
           V ERGAIPALP K+AV NAIF+TVS
Sbjct: 361 VKERGAIPALPTKDAVQNAIFETVS 384

BLAST of MC08g1777 vs. TAIR 10
Match: AT1G66430.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 564.3 bits (1453), Expect = 7.5e-161
Identity = 289/382 (75.65%), Postives = 325/382 (85.08%), Query Frame = 0

Query: 6   SAAFCFHG--VPNVTHELFTNRQWAVKSSFGSLRTRVVSESRLRVQVKAFSGDGVFAESN 65
           +  FCF G    +  H L + R  ++K++  S     +S SR  ++ +A S DG    + 
Sbjct: 7   TTTFCFSGPTFRSTPHSLTSKRPISIKATTSS--PSRLSNSRSNLKGRALSSDG---STQ 66

Query: 66  DSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKVGA 125
           +S  VVCFGEMLIDFVPTTSGLSLA+APAFKKAPGGAPANVAVGI+RLGGSSAFIGKVG 
Sbjct: 67  ESPYVVCFGEMLIDFVPTTSGLSLADAPAFKKAPGGAPANVAVGIARLGGSSAFIGKVGE 126

Query: 126 DEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQESE 185
           DEFGYMLA+ILK+NNV N+GM FDPGARTALAFVTL N+GEREFMFYRNPSADMLL+ESE
Sbjct: 127 DEFGYMLANILKDNNVNNDGMRFDPGARTALAFVTLTNEGEREFMFYRNPSADMLLEESE 186

Query: 186 LNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADSAI 245
           L++DLI+KAKIFHYGSISLITEPCKSAHI+AAKAAK+AG +LSYDPNLRLPLWPSAD+A 
Sbjct: 187 LDFDLIKKAKIFHYGSISLITEPCKSAHISAAKAAKEAGVILSYDPNLRLPLWPSADNAR 246

Query: 246 EGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYYTK 305
           E ILSIW+ ADIIKISEEEI FLT+GEDP+DD VVRKLFHP LKLLLVTEGP GCRYYTK
Sbjct: 247 EEILSIWETADIIKISEEEIVFLTKGEDPYDDNVVRKLFHPKLKLLLVTEGPEGCRYYTK 306

Query: 306 EFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALTVM 365
           +FSGRV GLKVD VDTTGAGDAFVAGILSQLA DLSLLQ E++LR+AL+FANACGALTV 
Sbjct: 307 DFSGRVHGLKVDVVDTTGAGDAFVAGILSQLANDLSLLQDEERLREALMFANACGALTVK 366

Query: 366 ERGAIPALPNKEAVLNAIFKTV 386
            RGAIPALP KEAV  A+ K V
Sbjct: 367 VRGAIPALPTKEAVHEALLKAV 383

BLAST of MC08g1777 vs. TAIR 10
Match: AT5G51830.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 477.6 bits (1228), Expect = 9.2e-135
Identity = 238/325 (73.23%), Postives = 281/325 (86.46%), Query Frame = 0

Query: 63  NDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKVG 122
           +  +LVVCFGEMLIDFVPT  G+SLAEAPAFKKAPGGAPANVAVG+SRLGGSSAFIGKVG
Sbjct: 19  DSETLVVCFGEMLIDFVPTVGGVSLAEAPAFKKAPGGAPANVAVGVSRLGGSSAFIGKVG 78

Query: 123 ADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQES 182
            DEFG MLADIL+ NNV N GM FD  ARTALAFVTLR DGEREF+F+R+PSADMLL ES
Sbjct: 79  DDEFGRMLADILRLNNVDNSGMRFDHNARTALAFVTLRGDGEREFLFFRHPSADMLLLES 138

Query: 183 ELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADSA 242
           EL+ +LI+KAKIFHYGSISLI EPC+S  + A K AK AG++LSYDPNLRLPLWPS ++A
Sbjct: 139 ELDKNLIQKAKIFHYGSISLIEEPCRSTQLVAMKIAKAAGSLLSYDPNLRLPLWPSEEAA 198

Query: 243 IEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVV-RKLFHPNLKLLLVTEGPGGCRYY 302
            + I+SIW++AD+IKISE+EI FLT G+DP+DD VV +KLFHPNLKLL+V+EGP GCRYY
Sbjct: 199 RKEIMSIWNLADVIKISEDEITFLTGGDDPYDDDVVLQKLFHPNLKLLVVSEGPNGCRYY 258

Query: 303 TKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALT 362
           T+EF GRV G+KV  VDTTGAGDAFV+G+L+ LA+DL+LL+ E KLR+ALLFANACGA+T
Sbjct: 259 TQEFKGRVGGVKVKPVDTTGAGDAFVSGLLNSLASDLTLLKDEKKLREALLFANACGAIT 318

Query: 363 VMERGAIPALPNKEAVLNAIFKTVS 387
           V ERGAIPA+P+ +AV + +  T S
Sbjct: 319 VTERGAIPAMPSMDAVQDLLSSTRS 343

BLAST of MC08g1777 vs. TAIR 10
Match: AT4G10260.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 427.9 bits (1099), Expect = 8.4e-120
Identity = 213/309 (68.93%), Postives = 252/309 (81.55%), Query Frame = 0

Query: 64  DSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIGKVGA 123
           ++ L+V FGEMLIDFVP TSG+SLAE+  F KAPGGAPANVA  I++LGG SAFIGK G 
Sbjct: 3   NTPLIVSFGEMLIDFVPDTSGVSLAESTGFLKAPGGAPANVACAITKLGGKSAFIGKFGD 62

Query: 124 DEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLLQESE 183
           DEFG+ML +ILK+N V +EG+ FD  ARTALAFVTL+ DGEREFMFYRNPSADMLL+ESE
Sbjct: 63  DEFGHMLVNILKKNGVNSEGVCFDTNARTALAFVTLKKDGEREFMFYRNPSADMLLKESE 122

Query: 184 LNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSADSAI 243
           LN DLI+KAKIFHYGSISLI+EPC++AH+AA K AKDAG +LSYDPN+RLPLWPS ++AI
Sbjct: 123 LNKDLIKKAKIFHYGSISLISEPCRTAHMAAMKTAKDAGVLLSYDPNVRLPLWPSTEAAI 182

Query: 244 EGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCRYYTK 303
           EGI SIW+ ADIIK+S++E+ FLTRG+   DD VV  L H  LKLL+VT+G  GCRYYTK
Sbjct: 183 EGIKSIWNEADIIKVSDDEVTFLTRGDAEKDD-VVLSLMHDKLKLLIVTDGEKGCRYYTK 242

Query: 304 EFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGALTVM 363
           +F GRV G  V AVDTTGAGD+FV   L  L  D S+L  E KL++AL FANACGA+   
Sbjct: 243 KFKGRVPGYAVKAVDTTGAGDSFVGAFLVSLGKDGSILDDEGKLKEALAFANACGAVCTT 302

Query: 364 ERGAIPALP 373
           ++GAIPALP
Sbjct: 303 QKGAIPALP 310

BLAST of MC08g1777 vs. TAIR 10
Match: AT3G59480.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 424.9 bits (1091), Expect = 7.1e-119
Identity = 210/318 (66.04%), Postives = 252/318 (79.25%), Query Frame = 0

Query: 60  AESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIG 119
           A + + SL+V FGEMLIDFVPT SG+SLA+AP F KAPGGAPANVA+ ISRLGG +AF+G
Sbjct: 3   ASNGEKSLIVSFGEMLIDFVPTVSGVSLADAPGFIKAPGGAPANVAIAISRLGGRAAFVG 62

Query: 120 KVGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLL 179
           K+G DEFG+MLA ILK+N V  EG+ FD GARTALAFVTLR+DGEREFMFYRNPSADMLL
Sbjct: 63  KLGDDEFGHMLAGILKQNGVSAEGINFDTGARTALAFVTLRSDGEREFMFYRNPSADMLL 122

Query: 180 QESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA 239
           +  ELN D+IR AK+FHYGSISLI EPC+SAH+ A + AK+AGA+LSYDPNLRLPLWPS 
Sbjct: 123 RPDELNLDVIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGALLSYDPNLRLPLWPSK 182

Query: 240 DSAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCR 299
           + A + ILSIWD A++IK+S+EE+ FLT G D  DD     L+H NLKLLLVT G  GCR
Sbjct: 183 EEAQKQILSIWDKAEVIKVSDEELMFLT-GSDKVDDETALSLWHSNLKLLLVTLGEKGCR 242

Query: 300 YYTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGA 359
           YYTK F G V    VDAVDTTGAGD+FV  +L ++  D ++L+ E +LR+ L  ANACGA
Sbjct: 243 YYTKSFRGSVDPFHVDAVDTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRLANACGA 302

Query: 360 LTVMERGAIPALPNKEAV 378
           +T  ++GAIPALP +  V
Sbjct: 303 ITTTKKGAIPALPTESEV 319

BLAST of MC08g1777 vs. TAIR 10
Match: AT2G31390.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 422.9 bits (1086), Expect = 2.7e-118
Identity = 210/324 (64.81%), Postives = 255/324 (78.70%), Query Frame = 0

Query: 60  AESNDSSLVVCFGEMLIDFVPTTSGLSLAEAPAFKKAPGGAPANVAVGISRLGGSSAFIG 119
           A + D  L+V FGEMLIDFVPT SG+SLAEAP F KAPGGAPANVA+ +SRLGG SAF+G
Sbjct: 2   ASNGDKGLIVSFGEMLIDFVPTESGVSLAEAPGFLKAPGGAPANVAIAVSRLGGRSAFVG 61

Query: 120 KVGADEFGYMLADILKENNVRNEGMLFDPGARTALAFVTLRNDGEREFMFYRNPSADMLL 179
           K+G DEFG+MLA IL++N V ++G+ FD GARTALAFVTLR DG+REFMFYRNPSADMLL
Sbjct: 62  KLGDDEFGHMLAGILRKNGVDDQGINFDTGARTALAFVTLRADGDREFMFYRNPSADMLL 121

Query: 180 QESELNYDLIRKAKIFHYGSISLITEPCKSAHIAAAKAAKDAGAVLSYDPNLRLPLWPSA 239
           +  ELN DLIR AK+FHYGSISLI EPC+SAH+ A + AK+AGA+LSYDPNLR PLWPS 
Sbjct: 122 RPDELNLDLIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGALLSYDPNLREPLWPSK 181

Query: 240 DSAIEGILSIWDIADIIKISEEEIKFLTRGEDPFDDAVVRKLFHPNLKLLLVTEGPGGCR 299
           + A   I+SIWD A+IIK+S+ E++FLT G +  DD     L+HPNLKLLLVT G  GCR
Sbjct: 182 EEAKTQIMSIWDKAEIIKVSDVELEFLT-GSNKIDDETALTLWHPNLKLLLVTLGEKGCR 241

Query: 300 YYTKEFSGRVKGLKVDAVDTTGAGDAFVAGILSQLATDLSLLQKEDKLRDALLFANACGA 359
           YYTK F G V    V+AVDTTGAGD+FV  +L+Q+  D S+L+ E++LR  L FANACGA
Sbjct: 242 YYTKTFKGAVDPFHVNAVDTTGAGDSFVGALLNQIVDDRSVLEDEERLRKVLRFANACGA 301

Query: 360 LTVMERGAIPALPNKEAVLNAIFK 384
           +T  ++GAIPALP+   V + + K
Sbjct: 302 ITTTKKGAIPALPSDAEVRSFLEK 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C5241.1e-15975.65Probable fructokinase-6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g66... [more]
Q9FLH81.3e-13373.23Probable fructokinase-7 OS=Arabidopsis thaliana OX=3702 GN=At5g51830 PE=1 SV=1[more]
O826161.2e-11868.93Probable fructokinase-5 OS=Arabidopsis thaliana OX=3702 GN=At4g10260 PE=2 SV=1[more]
Q9M1B91.0e-11766.04Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1[more]
Q9SID03.8e-11764.81Probable fructokinase-1 OS=Arabidopsis thaliana OX=3702 GN=At2g31390 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022143431.13.24e-275100.00probable fructokinase-6, chloroplastic [Momordica charantia][more]
XP_038890970.12.65e-25393.49probable fructokinase-6, chloroplastic [Benincasa hispida][more]
XP_023526609.13.09e-25292.47probable fructokinase-6, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] >... [more]
XP_022982732.14.39e-25292.73probable fructokinase-6, chloroplastic [Cucurbita maxima][more]
KAG6581213.14.39e-25292.21putative fructokinase-6, chloroplastic, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
A0A6J1CQ821.57e-275100.00probable fructokinase-6, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1IXD22.12e-25292.73probable fructokinase-6, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114815... [more]
A0A6J1FA537.09e-25191.95probable fructokinase-6, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A1S3C5X95.57e-24891.43probable fructokinase-6, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103496836 P... [more]
A0A0A0L7Q61.86e-24691.17PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G345390 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G66430.17.5e-16175.65pfkB-like carbohydrate kinase family protein [more]
AT5G51830.19.2e-13573.23pfkB-like carbohydrate kinase family protein [more]
AT4G10260.18.4e-12068.93pfkB-like carbohydrate kinase family protein [more]
AT3G59480.17.1e-11966.04pfkB-like carbohydrate kinase family protein [more]
AT2G31390.12.7e-11864.81pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002139Ribokinase/fructokinasePRINTSPR00990RIBOKINASEcoord: 93..112
score: 46.11
coord: 289..300
score: 34.26
coord: 248..263
score: 27.78
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 64..384
e-value: 4.6E-105
score: 353.6
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 68..379
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 67..373
e-value: 8.5E-77
score: 258.4
NoneNo IPR availablePANTHERPTHR43085:SF38PUTATIVE-RELATEDcoord: 48..384
NoneNo IPR availablePANTHERPTHR43085HEXOKINASE FAMILY MEMBERcoord: 48..384
NoneNo IPR availableCDDcd01167bac_FRKcoord: 67..368
e-value: 2.30826E-120
score: 348.857
IPR002173Carbohydrate/purine kinase, PfkB, conserved sitePROSITEPS00583PFKB_KINASES_1coord: 98..122
IPR002173Carbohydrate/purine kinase, PfkB, conserved sitePROSITEPS00584PFKB_KINASES_2coord: 318..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1777.1MC08g1777.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046835 carbohydrate phosphorylation
biological_process GO:0006000 fructose metabolic process
biological_process GO:0019252 starch biosynthetic process
cellular_component GO:0005829 cytosol
molecular_function GO:0008865 fructokinase activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor