MC08g1756 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g1756
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	MC08: 25641717 .. 25643555 (+)
RNA-Seq Expression	MC08g1756
Synteny	MC08g1756

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGAAAGGACCAGTAAAAGTCATCTTTCTCAGAGTGAAGAGACCGATTGGCATTCGTTGTGGTAGCAGAAGAAGAAGAACATCAAAAATGGCGGATTCTAGAGCATCGGACGGCGAAAGAGGGAAACGAAGTCCGGTTAGCGGCAGAGGGAAGACCGAGGCCATGAACGTCATATTCTCTGATCCCCAACTTCTCGATTGCTATATTTGCTGCGAGCCCTTGTCCGCACCTGTTTTCCAGGTCCGATTAAGCTCTTTTCTGCTTGTTCATATGGATGTTTCTGATGCTTTGTTCTTCTCGCTCTGTTTTGTTTTTCTATTTTTGAGTTCCGTAGTTGGAATTTGGAGACCTTGAAATCCCCCTCTCTGTGTGGTTTTTATCTTCTCTTTTTGGAATTTGTAGTTTGGCCTGTGGATTTCAATGAAAGTTTTGGATATTGATTAAAGGAAAAAGTGTGGTTCGGTTGGTTATTTTGAGGAAATATGGCATAGTTATGAACTGTTCAGTGCTGGTTGAGCTTTTGTAGCCAAATATAATTATAGACTTGTGGTTTTAAATGTTTTGATGTTAACAATCTCCAAAAAATGAAGATTATTCTGGATATTATGGTGACAGAGTCGAGGAATTGTTGGAATTGAAATGAAATCTTAAGCTGAATTGCTTAATTTGTTACATTATCTCAAGTAAATTTCAAAATCAAAACATGTTTATTTGGTATTTTCTTCACCTTTCCATGCAAAATTTGGGGCTCAATCTTGTGGATTATAGGATTTGAAACATCAATATAGAAGAAAATGAGGCTTGCATTTGTGTATATAATGTCTTGTTTGTTTGTTGCTCATGAAACTATATATTTTTTTTCATACTATGATTGAATGTTGGTGTTGGATGAATTAGTGTGAGAATGGGCACATTGCTTGCTCTAGCTGCTGCACCAAAGTTCAGAATAAATGCCCTAGCTGCAGCTTGTCAATTGGTTATATACGTTGCAGAGCGATCGAAAAGGTTCTTGAATCAATCAAGATACCCTGCCACAACTCAATATACGGATGCAAAATGACAATGACTTTGAACGAGAGAAATGACCATGGGTCACTGTGTACATATGAACCTTGCTCGTGCCCGGTATCCGATTGCTTATTTGTTGGTTCGTCCGAGAAGTTGTGCTCACACTTCAGCAAAAAGCACAAGAACTCAGCCAAAAGCTTCTCATACAATTCGAGGTTCACTCTATGCTTGAACAGTGGTGATAGTTACCGCATCCTTCAAGCGGAGAAAGATGGAGCTTTGTTCTTCCTCTCTTTCACTTTTGAAATCTTCGGGAATGCGGTAACTGTGAGTCGGATTGGACCATCGTCGTCTGAGAAAAAGTTTTGCTATGAAATCAAAGCGAAAACACAGGGGAGCATTCTTTGTTTGCAATCAATCGCGAAGGAGATCCAAGGTCGGGTTGAAGTTCCTCCTTCCAACAGATCACTTTTGATTCCGAACGAGTTCTTTGGATCTTCAGCCCAGACGATCTTGGAGTTTAGGATTTGGCCTTCATACTGAAAACAAACACTGGGGATCAACACAGGTGCACATACTTTTGCAAGAAAGCTGATTATTTATAAACTGAGTTGAATTATGTTGGCTGGATTTGTTCTTGGTTACCTAATCTTTGCATCTGAATTTGTGTTTATTTATTTTTTTTTTTGCAAAGGACTTGGTGCCTACTTTTGTACATCTTATCATATTTTGTCTTAGATATCTTGAAATATGAAGCATTGCATTTTGTATTTGAGGAAAGATGTATGTTTATAGAGGAAAGATGTATGTTTATAGGAAGATAACTTAACT

mRNA sequence

CAGAAAGGACCAGTAAAAGTCATCTTTCTCAGAGTGAAGAGACCGATTGGCATTCGTTGTGGTAGCAGAAGAAGAAGAACATCAAAAATGGCGGATTCTAGAGCATCGGACGGCGAAAGAGGGAAACGAAGTCCGGTTAGCGGCAGAGGGAAGACCGAGGCCATGAACGTCATATTCTCTGATCCCCAACTTCTCGATTGCTATATTTGCTGCGAGCCCTTGTCCGCACCTGTTTTCCAGTGTGAGAATGGGCACATTGCTTGCTCTAGCTGCTGCACCAAAGTTCAGAATAAATGCCCTAGCTGCAGCTTGTCAATTGGTTATATACGTTGCAGAGCGATCGAAAAGGTTCTTGAATCAATCAAGATACCCTGCCACAACTCAATATACGGATGCAAAATGACAATGACTTTGAACGAGAGAAATGACCATGGGTCACTGTGTACATATGAACCTTGCTCGTGCCCGGTATCCGATTGCTTATTTGTTGGTTCGTCCGAGAAGTTGTGCTCACACTTCAGCAAAAAGCACAAGAACTCAGCCAAAAGCTTCTCATACAATTCGAGGTTCACTCTATGCTTGAACAGTGGTGATAGTTACCGCATCCTTCAAGCGGAGAAAGATGGAGCTTTGTTCTTCCTCTCTTTCACTTTTGAAATCTTCGGGAATGCGGTAACTGTGAGTCGGATTGGACCATCGTCGTCTGAGAAAAAGTTTTGCTATGAAATCAAAGCGAAAACACAGGGGAGCATTCTTTGTTTGCAATCAATCGCGAAGGAGATCCAAGGTCGGGTTGAAGTTCCTCCTTCCAACAGATCACTTTTGATTCCGAACGAGTTCTTTGGATCTTCAGCCCAGACGATCTTGGAGTTTAGGATTTGGCCTTCATACTGAAAACAAACACTGGGGATCAACACAGGTGCACATACTTTTGCAAGAAAGCTGATTATTTATAAACTGAGTTGAATTATGTTGGCTGGATTTGTTCTTGGTTACCTAATCTTTGCATCTGAATTTGTGTTTATTTATTTTTTTTTTTGCAAAGGACTTGGTGCCTACTTTTGTACATCTTATCATATTTTGTCTTAGATATCTTGAAATATGAAGCATTGCATTTTGTATTTGAGGAAAGATGTATGTTTATAGAGGAAAGATGTATGTTTATAGGAAGATAACTTAACT

Coding sequence (CDS)

ATGGCGGATTCTAGAGCATCGGACGGCGAAAGAGGGAAACGAAGTCCGGTTAGCGGCAGAGGGAAGACCGAGGCCATGAACGTCATATTCTCTGATCCCCAACTTCTCGATTGCTATATTTGCTGCGAGCCCTTGTCCGCACCTGTTTTCCAGTGTGAGAATGGGCACATTGCTTGCTCTAGCTGCTGCACCAAAGTTCAGAATAAATGCCCTAGCTGCAGCTTGTCAATTGGTTATATACGTTGCAGAGCGATCGAAAAGGTTCTTGAATCAATCAAGATACCCTGCCACAACTCAATATACGGATGCAAAATGACAATGACTTTGAACGAGAGAAATGACCATGGGTCACTGTGTACATATGAACCTTGCTCGTGCCCGGTATCCGATTGCTTATTTGTTGGTTCGTCCGAGAAGTTGTGCTCACACTTCAGCAAAAAGCACAAGAACTCAGCCAAAAGCTTCTCATACAATTCGAGGTTCACTCTATGCTTGAACAGTGGTGATAGTTACCGCATCCTTCAAGCGGAGAAAGATGGAGCTTTGTTCTTCCTCTCTTTCACTTTTGAAATCTTCGGGAATGCGGTAACTGTGAGTCGGATTGGACCATCGTCGTCTGAGAAAAAGTTTTGCTATGAAATCAAAGCGAAAACACAGGGGAGCATTCTTTGTTTGCAATCAATCGCGAAGGAGATCCAAGGTCGGGTTGAAGTTCCTCCTTCCAACAGATCACTTTTGATTCCGAACGAGTTCTTTGGATCTTCAGCCCAGACGATCTTGGAGTTTAGGATTTGGCCTTCATACTGA

Protein sequence

MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPPSNRSLLIPNEFFGSSAQTILEFRIWPSY

Homology

[Close]

BLAST of MC08g1756 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.4e-49
Identity = 99/252 (39.29%), Postives = 143/252 (56.75%), Query Frame = 0


Query: 15  SPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCS 74
           S  S   K   ++V   DP +LDC ICCEPL  P+FQC+NGH+AC+ CCTKV+N+CPSC+
Sbjct: 90  SQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCT 149

Query: 75  LSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFV 134
           L IGY+RCRA+EKV+E+ ++ C N+ YGCK + +   R  H  +C + PCSCP+ DC + 
Sbjct: 150 LPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYT 209

Query: 135 GSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGN 194
           G  + L +H   +HK+   SF +N+R T+ L+      ILQ E DG +  +     +   
Sbjct: 210 GYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQEENDGHVIVVQVFRALHAV 269

Query: 195 AVTVSRIGP-SSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPPSNRSLLIPNEFFG 254
            V+VS I P +    +    +   T  S+L    + K IQ      P +  +LIP+  F 
Sbjct: 270 YVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQKVTNEHPEDGFMLIPSYLF- 329

Query: 255 SSAQTILEFRIW 266
            S    L  +IW
Sbjct: 330 -SGNDNLNLQIW 339

[Close]

BLAST of MC08g1756 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.2e-38
Identity = 85/228 (37.28%), Postives = 128/228 (56.14%), Query Frame = 0


Query: 27  NVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRCRAIE 86
           + + +D  +LDC IC + L  PVFQC NGH+ACSSCC K++NKCP+C+L +G+IRCRA+E
Sbjct: 34  SAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPVGHIRCRAME 93

Query: 87  KVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCSHFSK 146
           +VLES+ +PC  +  GC  T+     + H  +C + PCSCPV  C + GS + L  H+  
Sbjct: 94  RVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEHYDL 153

Query: 147 KHKNSAKSFSYN--SRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRIGPS 206
            H   + ++S+N  S     +   D   I +  +   LF +    E  G  V+VS I PS
Sbjct: 154 THSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCIAPS 213

Query: 207 SSE-KKFCYEIKAKT-QGSILCLQS-IAKEIQGRVEVPPSNRSLLIPN 250
           + E  +F Y +   T +G  +  QS   K++       P +  +LIP+
Sbjct: 214 APEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPH 261

[Close]

BLAST of MC08g1756 vs. ExPASy Swiss-Prot
Match: Q9FKD5 (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.6e-37
Identity = 89/256 (34.77%), Postives = 137/256 (53.52%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACS 60
           ++++  S G+ G+R       K +   ++  D  +LDC ICCE L++P+FQC+NGH+AC 
Sbjct: 6   ISEALISQGDGGERV-----AKRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACG 65

Query: 61  SCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCT 120
           SCC K+ NKCP+C+L +G+ R RA+E VLESI IPC N  +GC  +    + + H   C 
Sbjct: 66  SCCPKLSNKCPACTLPVGHSRSRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECI 125

Query: 121 YEPCSCPVSDCLFVGSSEKLCSHFSKKHKN----SAKSFSYNSRFTLCLNSGDSYRILQA 180
           +  CSCP S C + GS + L +H+   H      + K F   + FT  +   D   I + 
Sbjct: 126 FSQCSCPSSVCDYTGSYKDLYAHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRV 185

Query: 181 EKDGALFFLSFTFEIFGNAVTVSRIGPSSSE-KKFCYEIKAKTQGSILCLQS--IAKEIQ 240
            +   L  +    E  G  VTVS I PS+ E  +F Y++     G  +  +S  + +  +
Sbjct: 186 HEKKLLLAVQCFREPCGVYVTVSFIAPSAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCK 245

Query: 241 GRVEVPPSNRSLLIPN 250
             +E P  N  +LIP+
Sbjct: 246 VSIETPQEN-FMLIPH 255

[Close]

BLAST of MC08g1756 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.3e-36
Identity = 86/243 (35.39%), Postives = 128/243 (52.67%), Query Frame = 0


Query: 27  NVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRCRAIE 86
           + +  D ++LDC IC E  + P+FQC+NGH+ACSSCC K+ NKCP+C+  +G+ RCRA+E
Sbjct: 40  STMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGHNRCRAME 99

Query: 87  KVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCSHFSK 146
            VLESI IPC N+  GCK  ++  +   H   C +  C+CP  DC +  S + L +H+  
Sbjct: 100 SVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKDLYTHYRI 159

Query: 147 KHK--NSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRIGPS 206
            H   N   +F  +   ++ +N      I        LF +    E +G  VTVS I PS
Sbjct: 160 THMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYGVYVTVSCIAPS 219

Query: 207 SSE-KKFCYEIKAKTQGSILCLQS--IAKEIQGRVEVPPSNRSLLIPNEFFGSSAQTILE 265
           S E  ++ Y +     G  +  QS  + + ++   + P  N  +LIPN         +LE
Sbjct: 220 SPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQEN-FMLIPNSLLRGD---VLE 278

[Close]

BLAST of MC08g1756 vs. ExPASy Swiss-Prot
Match: Q9C9M0 (E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g66650 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 5.1e-36
Identity = 89/232 (38.36%), Postives = 122/232 (52.59%), Query Frame = 0


Query: 27  NVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRCRAIE 86
           +V   +  +L+C  C +PL  P+FQC NGH+AC  CC K++ +C  C L IG +RCRA+E
Sbjct: 75  SVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRCRAME 134

Query: 87  KVLESIKIPCHNSIYGCKMTMTL-NERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCSHFS 146
           KV+++  + C N+IYGCK + T  N+   H  +C + PCSCP+ DC ++G  + L +HF 
Sbjct: 135 KVIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLINHFR 194

Query: 147 KKHKNS---AKSFSYNSR--FTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSR 206
             HK S     SF ++    F L L+S D   I   EK G LF +       G   TVS 
Sbjct: 195 ATHKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGSHGVYATVSH 254

Query: 207 IGPSSSE-KKFCYEIKAKTQGSILCLQSIAKEIQ--GRVEVPPSNRSLLIPN 250
           I P   E +KF   +      S L L    K IQ     E  P    LLIP+
Sbjct: 255 IAPMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIPS 306

[Close]

BLAST of MC08g1756 vs. NCBI nr
Match: XP_022143325.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 552 bits (1423), Expect = 1.45e-198
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACS 60
           MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACS
Sbjct: 1   MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACS 60

Query: 61  SCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCT 120
           SCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCT
Sbjct: 61  SCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCT 120

Query: 121 YEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDG 180
           YEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDG
Sbjct: 121 YEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDG 180

Query: 181 ALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPP 240
           ALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPP
Sbjct: 181 ALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPP 240

Query: 241 SNRSLLIPNEFFGSSAQTILEFRIWPSY 268
           SNRSLLIPNEFFGSSAQTILEFRIWPSY
Sbjct: 241 SNRSLLIPNEFFGSSAQTILEFRIWPSY 268

[Close]

BLAST of MC08g1756 vs. NCBI nr
Match: XP_038893917.1 (E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida] >XP_038893986.1 E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida])

HSP 1 Score: 422 bits (1084), Expect = 6.90e-147
Identity = 204/269 (75.84%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPV--SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIA 60
           MADSR SD E  +R  +  S R K EAMNVIF+DPQ+LDCYICCE LS PVFQCENGHI+
Sbjct: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60

Query: 61  CSSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSL 120
           CSSCCTK+QNKCPSC+L+IG IRCRAIEKVLESIKIPC N+IYGCKMTM+LNE  DH + 
Sbjct: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120

Query: 121 CTYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEK 180
           CT+EPC CP+ DC FVGS+EKL  HFSKKHKN++KSFSYN+RFT+CLN+GDS+RILQAE 
Sbjct: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180

Query: 181 DGALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEV 240
           D  LF LS++FEIFGNAVTV+RIGP SSE+KFCYEIKAKTQGS+LC QSIAKEIQG  +V
Sbjct: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240

Query: 241 PPSNRSLLIPNEFFGSSAQTILEFRIWPS 267
           PPS   LLIPNE+FGSS QTILE RIWP+
Sbjct: 241 PPSKGWLLIPNEYFGSSTQTILEIRIWPA 268

[Close]

BLAST of MC08g1756 vs. NCBI nr
Match: XP_004140738.2 (E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus] >KGN57437.1 hypothetical protein Csa_011184 [Cucumis sativus])

HSP 1 Score: 422 bits (1084), Expect = 6.90e-147
Identity = 201/269 (74.72%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPV-SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60
           M DSR+SDG   +RS V S RG    +NVIF+DPQ+LDCYICCEPLS PVFQCENGHIAC
Sbjct: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60

Query: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120
           SSCCTK QNKCPSC+L+IGYIRCRAIEKVLESIK+PC N+IYGCK  M LN  NDH SLC
Sbjct: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120

Query: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180
            YEPCSCP+ +C FVGS+E+L  HF+KKHK+SAK FSYN+RFT+CLN+GD++RIL+AE D
Sbjct: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180

Query: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240
           G LFFLS+TFEIFGNAVT++RIGP SSEKKFCYEIKAKT GS+L LQSIAKEIQG ++VP
Sbjct: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240

Query: 241 PSNRSLLIPNEFFGSSAQTILEFRIWPSY 268
           PS  SLLIPNE+FGSS QT+LE  IWP++
Sbjct: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPAH 269

[Close]

BLAST of MC08g1756 vs. NCBI nr
Match: XP_022997791.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima])

HSP 1 Score: 413 bits (1062), Expect = 1.60e-143
Identity = 201/269 (74.72%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPVS-GRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60
           MADSR+SDGE+ +R  V+  RGK EAM VIF+DPQ+LDCYICCEPLS PV+QCENGHI+C
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120
           SSCC+KV NKCPSCSL+IGYIRCRAIEKVLESIKIPC NS YGCK+TM LN+ N+H + C
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180
            YEPCSCP+SDC +V S+E L SHF K+H++SAKSF YNSRFT+CLNSGD YRI QAE D
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240
            ALF LS++FEIFGNAVT+SRIG   SEKKFCY+IKAK+QGS+L LQS+AKEIQ   E P
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PSNRSLLIPNEFFGSS-AQTILEFRIWPS 267
           P   SLLIPNEFFGSS AQTILE RIWPS
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of MC08g1756 vs. NCBI nr
Match: KAG6606996.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 410 bits (1053), Expect = 3.75e-142
Identity = 198/269 (73.61%), Postives = 228/269 (84.76%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPVS-GRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60
           MADSR+SDGE+ +R  V+  RGK EAM VIF+DPQ+LDCYICCEPLS PV+QCENGHI+C
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120
           SSCC+KV NKCPSCSL+IGYIRCRAIEKVLESIKIPC NS YGCK+TM LN+ N+H + C
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180
            YEPCSCP+SDC +V S+E L SHF K+H++SAKSF YNSRFT+CLNSGD YRI QAE D
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240
            ALF LS++FEIFGNAVT+SRIG    EKKFCY+IKAK+QGS+L LQS+A EI+  +E P
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PSNRSLLIPNEFFGSS-AQTILEFRIWPS 267
           P   SLLIPNEFFGSS AQTILE RIWPS
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of MC08g1756 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 552 bits (1423), Expect = 7.01e-199
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACS 60
           MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACS
Sbjct: 1   MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACS 60

Query: 61  SCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCT 120
           SCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCT
Sbjct: 61  SCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCT 120

Query: 121 YEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDG 180
           YEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDG
Sbjct: 121 YEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDG 180

Query: 181 ALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPP 240
           ALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPP
Sbjct: 181 ALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPP 240

Query: 241 SNRSLLIPNEFFGSSAQTILEFRIWPSY 268
           SNRSLLIPNEFFGSSAQTILEFRIWPSY
Sbjct: 241 SNRSLLIPNEFFGSSAQTILEFRIWPSY 268

[Close]

BLAST of MC08g1756 vs. ExPASy TrEMBL
Match: A0A0A0L9Z6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G185660 PE=3 SV=1)

HSP 1 Score: 422 bits (1084), Expect = 3.34e-147
Identity = 201/269 (74.72%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPV-SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60
           M DSR+SDG   +RS V S RG    +NVIF+DPQ+LDCYICCEPLS PVFQCENGHIAC
Sbjct: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60

Query: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120
           SSCCTK QNKCPSC+L+IGYIRCRAIEKVLESIK+PC N+IYGCK  M LN  NDH SLC
Sbjct: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120

Query: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180
            YEPCSCP+ +C FVGS+E+L  HF+KKHK+SAK FSYN+RFT+CLN+GD++RIL+AE D
Sbjct: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180

Query: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240
           G LFFLS+TFEIFGNAVT++RIGP SSEKKFCYEIKAKT GS+L LQSIAKEIQG ++VP
Sbjct: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240

Query: 241 PSNRSLLIPNEFFGSSAQTILEFRIWPSY 268
           PS  SLLIPNE+FGSS QT+LE  IWP++
Sbjct: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPAH 269

[Close]

BLAST of MC08g1756 vs. ExPASy TrEMBL
Match: A0A6J1K8G5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 PE=3 SV=1)

HSP 1 Score: 413 bits (1062), Expect = 7.75e-144
Identity = 201/269 (74.72%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPVS-GRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60
           MADSR+SDGE+ +R  V+  RGK EAM VIF+DPQ+LDCYICCEPLS PV+QCENGHI+C
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120
           SSCC+KV NKCPSCSL+IGYIRCRAIEKVLESIKIPC NS YGCK+TM LN+ N+H + C
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180
            YEPCSCP+SDC +V S+E L SHF K+H++SAKSF YNSRFT+CLNSGD YRI QAE D
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240
            ALF LS++FEIFGNAVT+SRIG   SEKKFCY+IKAK+QGS+L LQS+AKEIQ   E P
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PSNRSLLIPNEFFGSS-AQTILEFRIWPS 267
           P   SLLIPNEFFGSS AQTILE RIWPS
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of MC08g1756 vs. ExPASy TrEMBL
Match: A0A6J1GBP1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508 PE=3 SV=1)

HSP 1 Score: 408 bits (1048), Expect = 9.42e-138
Identity = 197/269 (73.23%), Postives = 227/269 (84.39%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPVS-GRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60
           MADSR+SDGE+ +R  V+  RGK EAM VIF+DPQ+LDCYICCEPLS PV+QCENGHI+C
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120
           SSCC+KV NKCPSCSL+IGYIRCRAIEKVLESIKIPC NS YGCK+TM LN+ N+H + C
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180
            YEPCSCP+SDC +V S+E L SHF K+H +SAKSF YNSRFT+CLNSGD YRI QAE D
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240
            A+F LS++FEIFGNAVT+SRIG    EKKFCY+IKAK+QGS+L LQS+A EI+  +E P
Sbjct: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PSNRSLLIPNEFFGSS-AQTILEFRIWPS 267
           P   SLLIPNEFFGSS AQTILE RIWPS
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of MC08g1756 vs. ExPASy TrEMBL
Match: A0A6J1KAW2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 PE=3 SV=1)

HSP 1 Score: 390 bits (1002), Expect = 1.05e-134
Identity = 190/269 (70.63%), Postives = 220/269 (81.78%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPV-SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60
           MA SR+SDGE+ +RS V S R K E M VI +DPQ+LDCYICC PLS PV+QCENGHI+C
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60

Query: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120
           SSCC+KV NKCPSCSL IGY+RCRAIEKVLESIK+PC NS YGCK T  LN+ N+H +LC
Sbjct: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180
            Y+PCSCP+SDC +V S+E L SHF K+H++SAKS SYNSRFT+CLNS D Y I QAE D
Sbjct: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240
           G LF LS++FE FG+AVT+ RIGP SSEKKFCY+IKAK+ G++L L S+AKEIQ   E P
Sbjct: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240

Query: 241 PSNRSLLIPNEFFGSS-AQTILEFRIWPS 267
           PS  SLLIPNEFFGSS AQTILE RIWPS
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of MC08g1756 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-50
Identity = 99/252 (39.29%), Postives = 143/252 (56.75%), Query Frame = 0


Query: 15  SPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCS 74
           S  S   K   ++V   DP +LDC ICCEPL  P+FQC+NGH+AC+ CCTKV+N+CPSC+
Sbjct: 90  SQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCT 149

Query: 75  LSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFV 134
           L IGY+RCRA+EKV+E+ ++ C N+ YGCK + +   R  H  +C + PCSCP+ DC + 
Sbjct: 150 LPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYT 209

Query: 135 GSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGN 194
           G  + L +H   +HK+   SF +N+R T+ L+      ILQ E DG +  +     +   
Sbjct: 210 GYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQEENDGHVIVVQVFRALHAV 269

Query: 195 AVTVSRIGP-SSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPPSNRSLLIPNEFFG 254
            V+VS I P +    +    +   T  S+L    + K IQ      P +  +LIP+  F 
Sbjct: 270 YVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQKVTNEHPEDGFMLIPSYLF- 329

Query: 255 SSAQTILEFRIW 266
            S    L  +IW
Sbjct: 330 -SGNDNLNLQIW 339

[Close]

BLAST of MC08g1756 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 159.8 bits (403), Expect = 3.0e-39
Identity = 85/228 (37.28%), Postives = 128/228 (56.14%), Query Frame = 0


Query: 27  NVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRCRAIE 86
           + + +D  +LDC IC + L  PVFQC NGH+ACSSCC K++NKCP+C+L +G+IRCRA+E
Sbjct: 34  SAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPVGHIRCRAME 93

Query: 87  KVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCSHFSK 146
           +VLES+ +PC  +  GC  T+     + H  +C + PCSCPV  C + GS + L  H+  
Sbjct: 94  RVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEHYDL 153

Query: 147 KHKNSAKSFSYN--SRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRIGPS 206
            H   + ++S+N  S     +   D   I +  +   LF +    E  G  V+VS I PS
Sbjct: 154 THSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCIAPS 213

Query: 207 SSE-KKFCYEIKAKT-QGSILCLQS-IAKEIQGRVEVPPSNRSLLIPN 250
           + E  +F Y +   T +G  +  QS   K++       P +  +LIP+
Sbjct: 214 APEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPH 261

[Close]

BLAST of MC08g1756 vs. TAIR 10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 157.9 bits (398), Expect = 1.1e-38
Identity = 89/256 (34.77%), Postives = 137/256 (53.52%), Query Frame = 0


Query: 1   MADSRASDGERGKRSPVSGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACS 60
           ++++  S G+ G+R       K +   ++  D  +LDC ICCE L++P+FQC+NGH+AC 
Sbjct: 6   ISEALISQGDGGERV-----AKRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACG 65

Query: 61  SCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCT 120
           SCC K+ NKCP+C+L +G+ R RA+E VLESI IPC N  +GC  +    + + H   C 
Sbjct: 66  SCCPKLSNKCPACTLPVGHSRSRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECI 125

Query: 121 YEPCSCPVSDCLFVGSSEKLCSHFSKKHKN----SAKSFSYNSRFTLCLNSGDSYRILQA 180
           +  CSCP S C + GS + L +H+   H      + K F   + FT  +   D   I + 
Sbjct: 126 FSQCSCPSSVCDYTGSYKDLYAHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRV 185

Query: 181 EKDGALFFLSFTFEIFGNAVTVSRIGPSSSE-KKFCYEIKAKTQGSILCLQS--IAKEIQ 240
            +   L  +    E  G  VTVS I PS+ E  +F Y++     G  +  +S  + +  +
Sbjct: 186 HEKKLLLAVQCFREPCGVYVTVSFIAPSAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCK 245

Query: 241 GRVEVPPSNRSLLIPN 250
             +E P  N  +LIP+
Sbjct: 246 VSIETPQEN-FMLIPH 255

[Close]

BLAST of MC08g1756 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 154.8 bits (390), Expect = 9.5e-38
Identity = 86/243 (35.39%), Postives = 128/243 (52.67%), Query Frame = 0


Query: 27  NVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRCRAIE 86
           + +  D ++LDC IC E  + P+FQC+NGH+ACSSCC K+ NKCP+C+  +G+ RCRA+E
Sbjct: 40  STMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGHNRCRAME 99

Query: 87  KVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCSHFSK 146
            VLESI IPC N+  GCK  ++  +   H   C +  C+CP  DC +  S + L +H+  
Sbjct: 100 SVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKDLYTHYRI 159

Query: 147 KHK--NSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRIGPS 206
            H   N   +F  +   ++ +N      I        LF +    E +G  VTVS I PS
Sbjct: 160 THMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYGVYVTVSCIAPS 219

Query: 207 SSE-KKFCYEIKAKTQGSILCLQS--IAKEIQGRVEVPPSNRSLLIPNEFFGSSAQTILE 265
           S E  ++ Y +     G  +  QS  + + ++   + P  N  +LIPN         +LE
Sbjct: 220 SPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQEN-FMLIPNSLLRGD---VLE 278

[Close]

BLAST of MC08g1756 vs. TAIR 10
Match: AT1G66650.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 152.9 bits (385), Expect = 3.6e-37
Identity = 89/232 (38.36%), Postives = 122/232 (52.59%), Query Frame = 0


Query: 27  NVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRCRAIE 86
           +V   +  +L+C  C +PL  P+FQC NGH+AC  CC K++ +C  C L IG +RCRA+E
Sbjct: 75  SVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRCRAME 134

Query: 87  KVLESIKIPCHNSIYGCKMTMTL-NERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCSHFS 146
           KV+++  + C N+IYGCK + T  N+   H  +C + PCSCP+ DC ++G  + L +HF 
Sbjct: 135 KVIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLINHFR 194

Query: 147 KKHKNS---AKSFSYNSR--FTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSR 206
             HK S     SF ++    F L L+S D   I   EK G LF +       G   TVS 
Sbjct: 195 ATHKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGSHGVYATVSH 254

Query: 207 IGPSSSE-KKFCYEIKAKTQGSILCLQSIAKEIQ--GRVEVPPSNRSLLIPN 250
           I P   E +KF   +      S L L    K IQ     E  P    LLIP+
Sbjct: 255 IAPMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIPS 306

The following BLAST results are available for this feature:

BLAST of MC08g1756 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84K34	2.4e-49	39.29	E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9FKD9	4.2e-38	37.28	Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FKD5	1.6e-37	34.77	Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FKD7	1.3e-36	35.39	E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3...	[more]
Q9C9M0	5.1e-36	38.36	E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g6...	[more]

BLAST of MC08g1756 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143325.1	1.45e-198	100.00	E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia]	[more]
XP_038893917.1	6.90e-147	75.84	E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida] >XP_038893986.1 E3 ...	[more]
XP_004140738.2	6.90e-147	74.72	E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus] >KGN57437.1 hypotheti...	[more]
XP_022997791.1	1.60e-143	74.72	E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima]	[more]
KAG6606996.1	3.75e-142	73.61	E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of MC08g1756 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNI1	7.01e-199	100.00	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]
A0A0A0L9Z6	3.34e-147	74.72	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G185660 PE...	[more]
A0A6J1K8G5	7.75e-144	74.72	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 P...	[more]
A0A6J1GBP1	9.42e-138	73.23	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508...	[more]
A0A6J1KAW2	1.05e-134	70.63	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 P...	[more]

BLAST of MC08g1756 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37930.1	1.7e-50	39.29	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37870.1	3.0e-39	37.28	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37910.1	1.1e-38	34.77	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37890.1	9.5e-38	35.39	Protein with RING/U-box and TRAF-like domains	[more]
AT1G66650.1	3.6e-37	38.36	Protein with RING/U-box and TRAF-like domains	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 86..144 e-value: 7.0E-14 score: 53.8
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 80..236 e-value: 6.8E-21 score: 75.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..18
None	No IPR available	PANTHER	PTHR46632:SF16	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 17..266
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 91..253
IPR044286	E3 ubiquitin-protein ligase SINA-like, plant	PANTHER	PTHR46632	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 17..266
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 91..149 score: 13.221327
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 38..74 score: 8.638745

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g1756.1	MC08g1756.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0008270	zinc ion binding