MC08g1738 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g1738
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	aspartic proteinase PCS1
Location	MC08: 25468654 .. 25469145 (+)
RNA-Seq Expression	MC08g1738
Synteny	MC08g1738

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCGCCTACACGCTCCCGCTGCTGGGGATCAAGATCGGCGGGAGGCCGCTCAACATCTCCGCCGCGGCGTTCCGGCCCGACCAGACCGGGTCCGGGCAGACCATGATCGACTCCGGCTCGGACCTGACGTATCTCGTGGGCGAGGCGTACGACAAGGTGAAAGCGGAGATCGTGAGTCTGGTGGGGCCCAGGATGAAGAAGGGTTACGTGTACGCCAACGTGGCCGACATGTGTTTCGACGGCGTTGACGCGGCGGCGGTGGGGCCCAGGATTGGCGACATGTCGTTCGTGTTTGCCAAGGGGGTGGAGATTGTGGTGCGGAATGAGAGGATTTTTACGGAGGTGGAGGCCGGAGTGAAGTGCGTGGGGATCGGACGGTCGGATTTGCTCGGGATTGCGAGTAATATAATCGGGAACGTCCATCAGCAAAATATGTGGGTGGAGTACGATGTGACCAATAGGAGAGTTGGGTTCGGTGAAGCTGACTGCAGC

mRNA sequence

TTCGCCTACACGCTCCCGCTGCTGGGGATCAAGATCGGCGGGAGGCCGCTCAACATCTCCGCCGCGGCGTTCCGGCCCGACCAGACCGGGTCCGGGCAGACCATGATCGACTCCGGCTCGGACCTGACGTATCTCGTGGGCGAGGCGTACGACAAGGTGAAAGCGGAGATCGTGAGTCTGGTGGGGCCCAGGATGAAGAAGGGTTACGTGTACGCCAACGTGGCCGACATGTGTTTCGACGGCGTTGACGCGGCGGCGGTGGGGCCCAGGATTGGCGACATGTCGTTCGTGTTTGCCAAGGGGGTGGAGATTGTGGTGCGGAATGAGAGGATTTTTACGGAGGTGGAGGCCGGAGTGAAGTGCGTGGGGATCGGACGGTCGGATTTGCTCGGGATTGCGAGTAATATAATCGGGAACGTCCATCAGCAAAATATGTGGGTGGAGTACGATGTGACCAATAGGAGAGTTGGGTTCGGTGAAGCTGACTGCAGC

Coding sequence (CDS)

TTCGCCTACACGCTCCCGCTGCTGGGGATCAAGATCGGCGGGAGGCCGCTCAACATCTCCGCCGCGGCGTTCCGGCCCGACCAGACCGGGTCCGGGCAGACCATGATCGACTCCGGCTCGGACCTGACGTATCTCGTGGGCGAGGCGTACGACAAGGTGAAAGCGGAGATCGTGAGTCTGGTGGGGCCCAGGATGAAGAAGGGTTACGTGTACGCCAACGTGGCCGACATGTGTTTCGACGGCGTTGACGCGGCGGCGGTGGGGCCCAGGATTGGCGACATGTCGTTCGTGTTTGCCAAGGGGGTGGAGATTGTGGTGCGGAATGAGAGGATTTTTACGGAGGTGGAGGCCGGAGTGAAGTGCGTGGGGATCGGACGGTCGGATTTGCTCGGGATTGCGAGTAATATAATCGGGAACGTCCATCAGCAAAATATGTGGGTGGAGTACGATGTGACCAATAGGAGAGTTGGGTTCGGTGAAGCTGACTGCAGC

Protein sequence

FAYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLVGPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVVRNERIFTEVEAGVKCVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS

Homology

[Close]

BLAST of MC08g1738 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 7.5e-22
Identity = 57/175 (32.57%), Postives = 94/175 (53.71%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVS-- 61
           AYT+ L GIK+ G+ L I  +   PD TG+GQTM+DSG+  T+L+G  Y  +++  ++  
Sbjct: 269 AYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRT 328

Query: 62  --LVGPRMKKGYVYANVADMCFDGVDA---AAVGPRIGDMSFVFAKGVEIVVRNERIFTE 121
             ++       +V+    D+C+        + +  R+  +S VF +G EI V  + +   
Sbjct: 329 NGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVF-EGAEIAVSGQPLLYR 388

Query: 122 V------EAGVKCVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADC 164
           V         V C   G SDL+G+ + +IG+ HQQNMW+E+D+   R+G    +C
Sbjct: 389 VPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVEC 442

[Close]

BLAST of MC08g1738 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.1e-12
Identity = 51/163 (31.29%), Postives = 77/163 (47.24%), Query Frame = 0


Query: 3   YTLPLLGIKIGGRPLNISAAAFR-PDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 62
           Y + L G+ +G   L I  +AF      G+G  +IDSG+ LTY V  AY  V+ E +S +
Sbjct: 280 YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQI 339

Query: 63  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVF-AKGVEIVVRNERIFTEVEAGVK 122
              +  G   ++  D+CF      +    +   +FV    G ++ + +E  F     G+ 
Sbjct: 340 NLPVVNG--SSSGFDLCF---QTPSDPSNLQIPTFVMHFDGGDLELPSENYFISPSNGLI 399

Query: 123 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADC 164
           C+ +G S       +I GN+ QQNM V YD  N  V F  A C
Sbjct: 400 CLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of MC08g1738 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.6e-11
Identity = 52/163 (31.90%), Postives = 80/163 (49.08%), Query Frame = 0


Query: 3   YTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLVG 62
           Y + L GI +GG  L I ++ F+    G+G  +IDSG+ LTYL  +AY+ V       + 
Sbjct: 281 YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQIN 340

Query: 63  -PRMKKGYVYANVADMCF-DGVDAAAVGPRIGDMSFVFAKGVEIVVRNERIFTEVEAGVK 122
            P + +    ++    CF    D + V  ++ ++S  F  GV + +  + I      GV 
Sbjct: 341 LPTVDES---SSGLSTCFQQPSDGSTV--QVPEISMQFDGGV-LNLGEQNILISPAEGVI 400

Query: 123 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADC 164
           C+ +G S  LGI  +I GN+ QQ   V YD+ N  V F    C
Sbjct: 401 CLAMGSSSQLGI--SIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of MC08g1738 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 6.5e-10
Identity = 49/163 (30.06%), Postives = 78/163 (47.85%), Query Frame = 0


Query: 3   YTLPLLGIKIGG-RPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 62
           Y + LLGI +GG R   ++A+ F+ DQ G+G  +IDSG+ +T L+  AY  +       V
Sbjct: 330 YYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAY--IAMRDAFRV 389

Query: 63  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVVRNERIFTEVEAGVKC 122
           G +  K     ++ D CFD  +   V      + F   +G ++ +        V+   K 
Sbjct: 390 GAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF---RGADVSLPATNYLIPVDTNGKF 449

Query: 123 VGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 165
                  + G+  +IIGN+ QQ   V YD+ + RVGF    C+
Sbjct: 450 CFAFAGTMGGL--SIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of MC08g1738 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-09
Identity = 42/161 (26.09%), Postives = 75/161 (46.58%), Query Frame = 0


Query: 3   YTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLVG 62
           Y + L G  +GG  + +  A F  D +GSG  ++D G+ +T L  +AY+ ++   + L  
Sbjct: 345 YYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLT- 404

Query: 63  PRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVVRNERIFTEVEAGVKCV 122
             +KKG    ++ D C+D    + V        F   K +++  +N  I  + ++G  C 
Sbjct: 405 VNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVD-DSGTFCF 464

Query: 123 GIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADC 164
               +     + +IIGNV QQ   + YD++   +G     C
Sbjct: 465 AFAPT---SSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of MC08g1738 vs. NCBI nr
Match: KAG6606985.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 238 bits (607), Expect = 2.80e-74
Identity = 120/164 (73.17%), Postives = 134/164 (81.71%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GI+I    LNIS A F+PD +GSGQTMIDSGSDLTYLV EAY+KV+AEIV LV
Sbjct: 245 AYTLPMKGIRIAKNQLNISPAVFKPDPSGSGQTMIDSGSDLTYLVDEAYNKVRAEIVKLV 304

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           GP MKKGY YA+VADMCFDG  AAA G  IGDM F F  GVEI+V + E + TEVE GV+
Sbjct: 305 GPMMKKGYEYASVADMCFDGAVAAAAGQGIGDMWFEFENGVEILVGKGEGLLTEVEKGVR 364

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVGIGRSD LG  SNIIGNVHQQNMWVEYD+TN+RVGFG A+CS
Sbjct: 365 CVGIGRSDRLGTGSNIIGNVHQQNMWVEYDLTNKRVGFGGAECS 408

[Close]

BLAST of MC08g1738 vs. NCBI nr
Match: KAG6607567.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 233 bits (595), Expect = 2.99e-74
Identity = 119/164 (72.56%), Postives = 132/164 (80.49%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GI+IG   LNIS A F+PD +G+GQTMIDSGSDLTYLV EAY KV+ E+V LV
Sbjct: 106 AYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRLV 165

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           GP MKKGY YA VADMCFDG  AAAVG RIGDM F F  GVEI+V + E + TEVE GVK
Sbjct: 166 GPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGVK 225

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVGIGRSD L   SNIIGNVHQQNMWVEYD++N+RVGFG A CS
Sbjct: 226 CVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCS 269

[Close]

BLAST of MC08g1738 vs. NCBI nr
Match: XP_023525882.1 (aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 236 bits (602), Expect = 1.59e-73
Identity = 119/164 (72.56%), Postives = 132/164 (80.49%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GI+I    LNIS   F+PD +GSGQTMIDSGSDLTYLV EAY+KV+AEIV LV
Sbjct: 245 AYTLPMKGIRIAKNQLNISPTVFKPDPSGSGQTMIDSGSDLTYLVDEAYNKVRAEIVRLV 304

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           GP MKKGY YA+VADMCFDG  AAA G  IGDM F F  GVEI+V + E + TEVE GVK
Sbjct: 305 GPMMKKGYEYASVADMCFDGAVAAAAGQGIGDMWFEFENGVEILVGKGEGLLTEVEKGVK 364

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           C+GIGRSD LG  SNIIGNVHQQNMWVEYD TN+RVGFG A+CS
Sbjct: 365 CIGIGRSDRLGTGSNIIGNVHQQNMWVEYDFTNKRVGFGGAECS 408

[Close]

BLAST of MC08g1738 vs. NCBI nr
Match: XP_008457122.1 (PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0047379.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa] >TYK14056.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa])

HSP 1 Score: 236 bits (602), Expect = 2.51e-73
Identity = 120/164 (73.17%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GIKI G+ LNIS AAF+PD  GSGQTMIDSGSDLTYLV EAY+KVK E+V LV
Sbjct: 263 AYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLV 322

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           G +MKKGYVYA VADMCFD    A VG RIG +SF F  GVEI+V R E + TEVE GVK
Sbjct: 323 GAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFDNGVEILVGRGEGVLTEVEKGVK 382

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVG GRS+ LGI SNIIG VHQQNMWVEYD+TNRR+GFG A+CS
Sbjct: 383 CVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGFGGAECS 426

[Close]

BLAST of MC08g1738 vs. NCBI nr
Match: KAG6607601.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 233 bits (595), Expect = 3.02e-73
Identity = 119/164 (72.56%), Postives = 132/164 (80.49%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GI+IG   LNIS A F+PD +G+GQTMIDSGSDLTYLV EAY KV+ E+V LV
Sbjct: 182 AYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRLV 241

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           GP MKKGY YA VADMCFDG  AAAVG RIGDM F F  GVEI+V + E + TEVE GVK
Sbjct: 242 GPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGVK 301

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVGIGRSD L   SNIIGNVHQQNMWVEYD++N+RVGFG A CS
Sbjct: 302 CVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCS 345

[Close]

BLAST of MC08g1738 vs. ExPASy TrEMBL
Match: A0A5A7U1M2 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00330 PE=3 SV=1)

HSP 1 Score: 236 bits (602), Expect = 1.22e-73
Identity = 120/164 (73.17%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GIKI G+ LNIS AAF+PD  GSGQTMIDSGSDLTYLV EAY+KVK E+V LV
Sbjct: 263 AYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLV 322

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           G +MKKGYVYA VADMCFD    A VG RIG +SF F  GVEI+V R E + TEVE GVK
Sbjct: 323 GAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFDNGVEILVGRGEGVLTEVEKGVK 382

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVG GRS+ LGI SNIIG VHQQNMWVEYD+TNRR+GFG A+CS
Sbjct: 383 CVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGFGGAECS 426

[Close]

BLAST of MC08g1738 vs. ExPASy TrEMBL
Match: A0A1S3C4D2 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1)

HSP 1 Score: 236 bits (602), Expect = 1.22e-73
Identity = 120/164 (73.17%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GIKI G+ LNIS AAF+PD  GSGQTMIDSGSDLTYLV EAY+KVK E+V LV
Sbjct: 263 AYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLV 322

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           G +MKKGYVYA VADMCFD    A VG RIG +SF F  GVEI+V R E + TEVE GVK
Sbjct: 323 GAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEFDNGVEILVGRGEGVLTEVEKGVK 382

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVG GRS+ LGI SNIIG VHQQNMWVEYD+TNRR+GFG A+CS
Sbjct: 383 CVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIGFGGAECS 426

[Close]

BLAST of MC08g1738 vs. ExPASy TrEMBL
Match: A0A6J1F166 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438397 PE=3 SV=1)

HSP 1 Score: 232 bits (592), Expect = 9.29e-73
Identity = 118/164 (71.95%), Postives = 131/164 (79.88%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GI+IG   LNIS A F+PD +G+GQTMIDSGSDLTYLV EAY KV+ E+V LV
Sbjct: 210 AYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRLV 269

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           GP MKKGY YA VADMCFDG  AAAVG RIGDM F F  GVEI+V + E + TEVE GVK
Sbjct: 270 GPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGVK 329

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVGIGRSD L   SNIIGNVHQQNMWVEYD+ N+R+GFG A CS
Sbjct: 330 CVGIGRSDRLVTESNIIGNVHQQNMWVEYDLINKRIGFGVAKCS 373

[Close]

BLAST of MC08g1738 vs. ExPASy TrEMBL
Match: A0A6J1ICY2 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471416 PE=3 SV=1)

HSP 1 Score: 233 bits (595), Expect = 9.68e-73
Identity = 118/164 (71.95%), Postives = 132/164 (80.49%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GI+IG   LNIS A F+PD +G+GQTMIDSGSDLTYLV EAY KV+AE+V LV
Sbjct: 249 AYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVRAEMVRLV 308

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           GP MKKGY YA VADMCFDG  AA VG RIGDM F F  GVEI+V + E + TEVE GVK
Sbjct: 309 GPMMKKGYEYAAVADMCFDGAVAAVVGRRIGDMWFQFENGVEILVGKGEGLLTEVEKGVK 368

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVGIGRSD L   SNIIGNVHQQNMWVEYD++N+R+GFG A CS
Sbjct: 369 CVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCS 412

[Close]

BLAST of MC08g1738 vs. ExPASy TrEMBL
Match: A0A6J1ICW1 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471394 PE=3 SV=1)

HSP 1 Score: 233 bits (595), Expect = 1.08e-72
Identity = 118/164 (71.95%), Postives = 132/164 (80.49%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYTLP+ GI+IG   LNIS A F+PD +G+GQTMIDSGSDLTYLV EAY KV+AE+V LV
Sbjct: 253 AYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVRAEMVRLV 312

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVV-RNERIFTEVEAGVK 121
           GP MKKGY YA VADMCFDG  AA VG RIGDM F F  GVEI+V + E + TEVE GVK
Sbjct: 313 GPMMKKGYEYAAVADMCFDGAVAAVVGRRIGDMWFQFENGVEILVGKGEGLLTEVEKGVK 372

Query: 122 CVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 164
           CVGIGRSD L   SNIIGNVHQQNMWVEYD++N+R+GFG A CS
Sbjct: 373 CVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCS 416

[Close]

BLAST of MC08g1738 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 221.9 bits (564), Expect = 3.9e-58
Identity = 105/163 (64.42%), Postives = 129/163 (79.14%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYT+P++GI+ G + LNIS + FRPD  GSGQTM+DSGS+ T+LV  AYDKV+AEI++ V
Sbjct: 266 AYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRV 325

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVVRNERIFTEVEAGVKC 121
           G R+KKGYVY   ADMCFDG + A +   IGD+ FVF +GVEI+V  ER+   V  G+ C
Sbjct: 326 GRRLKKGYVYGGTADMCFDG-NVAMIPRLIGDLVFVFTRGVEILVPKERVLVNVGGGIHC 385

Query: 122 VGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADCS 165
           VGIGRS +LG ASNIIGNVHQQN+WVE+DVTNRRVGF +ADCS
Sbjct: 386 VGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADCS 427

[Close]

BLAST of MC08g1738 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 219.2 bits (557), Expect = 2.5e-57
Identity = 103/162 (63.58%), Postives = 125/162 (77.16%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYT+PL GI+IG + LNI  + FRPD  GSGQTM+DSGS+ T+LV  AYDKVK EIV LV
Sbjct: 277 AYTVPLQGIRIGQKRLNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLV 336

Query: 62  GPRMKKGYVYANVADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVVRNERIFTEVEAGVKC 121
           G R+KKGYVY + ADMCFDG  +  +G  IGD+ F F +GVEI+V  + +   V  G+ C
Sbjct: 337 GSRLKKGYVYGSTADMCFDGNHSMEIGRLIGDLVFEFGRGVEILVEKQSLLVNVGGGIHC 396

Query: 122 VGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADC 164
           VGIGRS +LG ASNIIGNVHQQN+WVE+DVTNRRVGF +A+C
Sbjct: 397 VGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAEC 438

[Close]

BLAST of MC08g1738 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.1e-23
Identity = 61/177 (34.46%), Postives = 97/177 (54.80%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLV 61
           AYT+ L GI++G + L++  + F PD TG+GQTM+DSG+  T+L+G  Y  +K E ++  
Sbjct: 258 AYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEFITQT 317

Query: 62  GPRMK----KGYVYANVADMC----------FDGVDAAAVGPRIGDMSFVFAKGVEIVVR 121
              ++      +V+    D+C          F G+   ++  R  +MS     G +++ R
Sbjct: 318 KSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMS---VSGQKLLYR 377

Query: 122 NERIFTEVEAGVKCVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGF-GEADC 164
                +E +  V C   G SDLLGI + +IG+ HQQN+W+E+D+   RVGF G   C
Sbjct: 378 VNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRC 431

[Close]

BLAST of MC08g1738 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 105.1 bits (261), Expect = 5.3e-23
Identity = 57/175 (32.57%), Postives = 94/175 (53.71%), Query Frame = 0


Query: 2   AYTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVS-- 61
           AYT+ L GIK+ G+ L I  +   PD TG+GQTM+DSG+  T+L+G  Y  +++  ++  
Sbjct: 269 AYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRT 328

Query: 62  --LVGPRMKKGYVYANVADMCFDGVDA---AAVGPRIGDMSFVFAKGVEIVVRNERIFTE 121
             ++       +V+    D+C+        + +  R+  +S VF +G EI V  + +   
Sbjct: 329 NGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVF-EGAEIAVSGQPLLYR 388

Query: 122 V------EAGVKCVGIGRSDLLGIASNIIGNVHQQNMWVEYDVTNRRVGFGEADC 164
           V         V C   G SDL+G+ + +IG+ HQQNMW+E+D+   R+G    +C
Sbjct: 389 VPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVEC 442

[Close]

BLAST of MC08g1738 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-10
Identity = 46/168 (27.38%), Postives = 74/168 (44.05%), Query Frame = 0


Query: 3   YTLPLLGIKIGGRPLNISAAAFRPDQTGSGQTMIDSGSDLTYLVGEAYDKVKAEIVSLVG 62
           Y + +  I +GG+ L+I    +     G G T+IDSG+ L+Y    AY+ +K +      
Sbjct: 367 YYIQIKSILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAE--- 426

Query: 63  PRMKKGY-VYAN--VADMCFDGVDAAAVGPRIGDMSFVFAKGVEIVVRNERIFTEVEAGV 122
            +MK+ Y ++ +  V D CF+          + ++   F  G       E  F  +   +
Sbjct: 427 -KMKENYPIFRDFPVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDL 486

Query: 123 KCVGIGRSDLLGIAS---NIIGNVHQQNMWVEYDVTNRRVGFGEADCS 165
            C+ I     LG      +IIGN  QQN  + YD    R+GF    C+
Sbjct: 487 VCLAI-----LGTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKCA 525

The following BLAST results are available for this feature:

BLAST of MC08g1738 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	7.5e-22	32.57	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C3	4.1e-12	31.29	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.6e-11	31.90	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	6.5e-10	30.06	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LS40	2.5e-09	26.09	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of MC08g1738 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606985.1	2.80e-74	73.17	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6607567.1	2.99e-74	72.56	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023525882.1	1.59e-73	72.56	aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo]	[more]
XP_008457122.1	2.51e-73	73.17	PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0047379.1 aspartic protei...	[more]
KAG6607601.1	3.02e-73	72.56	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MC08g1738 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U1M2	1.22e-73	73.17	Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3C4D2	1.22e-73	73.17	aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1	[more]
A0A6J1F166	9.29e-73	71.95	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438397 PE=3...	[more]
A0A6J1ICY2	9.68e-73	71.95	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471416 PE=3 S...	[more]
A0A6J1ICW1	1.08e-72	71.95	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111471394 PE=3 S...	[more]

BLAST of MC08g1738 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G66180.1	3.9e-58	64.42	Eukaryotic aspartyl protease family protein	[more]
AT5G37540.1	2.5e-57	63.58	Eukaryotic aspartyl protease family protein	[more]
AT2G39710.1	1.1e-23	34.46	Eukaryotic aspartyl protease family protein	[more]
AT5G02190.1	5.3e-23	32.57	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	1.8e-10	27.38	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 1..164 e-value: 1.0E-37 score: 131.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 2..163
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 2..159 e-value: 3.7E-35 score: 121.1
None	No IPR available	PANTHER	PTHR47965:SF51	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 1..164
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 1..164
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 1..159 score: 18.695501

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g1738.1	MC08g1738.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity