MC08g1720 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1720 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.6e-58
Identity = 159/454 (35.02%), Postives = 228/454 (50.22%), Query Frame = 0

Query: 5   IVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFY----HNNESSAGGRRRSH-S 64
           I  K  IKPSS TP  LR    S  D  +   Y+ C FFY       E S      SH +
Sbjct: 12  ICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSD------------- 124
             L+ SLS+ L  YYP+AG+L D A+V+CND GA F    ++C MS+             
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 VTKNDEIWKLLCPGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWAS 184
           V   D  W   C G       +L VQ+++F CGG AI V  SHKI D  +   F+NDW+S
Sbjct: 132 VLPKDLPWANNCEGGN-----LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSS 191

Query: 185 IARNHRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVK 244
           + R+H      L  SP+F   S F   +    +        +    S L E      V K
Sbjct: 192 VTRDHT--TTTLVPSPRFVGDSVFSTKKYGSLI--------TPQILSDLNE-----CVQK 251

Query: 245 RFAFESPKIAALKAMVSEK--VENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMV 304
           R  F + K+ AL+A V+E+  V+NPTR +++SAL++K A  ++       + PS L+  +
Sbjct: 252 RLIFPTDKLDALRAKVAEESGVKNPTRAEVVSALLFKCATKASSS-----MLPSKLVHFL 311

Query: 305 NIRKRVVPPLPEALKGNIITFFVVPL--QEEVELWEVVRDMKRNFEEFC--DKYPKN--- 364
           NIR  + P LP    GN+ + F +     +++EL  +VR++++  E     D+  +N   
Sbjct: 312 NIRTMIKPRLPRNAIGNLSSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELI 371

Query: 365 YEAVEWCSLHKLYMEESFGRMAQNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSI 424
            E VE     KL  E   G   +N Y CS+ C    Y VDFGWG+P  + + +   +N+ 
Sbjct: 372 LEVVESMREGKLPFENMDG--YENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAF 431

Query: 425 GLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSF 432
            L D K G+G+EA V L K+ M+ FE+N+ELL F
Sbjct: 432 FLKDYKAGQGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of MC08g1720 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 7.7e-58
Identity = 158/454 (34.80%), Postives = 227/454 (50.00%), Query Frame = 0

Query: 5   IVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFY----HNNESSAGGRRRSH-S 64
           +  K  IKPSS TP  LR    S  D  +   Y+ C FFY       E S      SH +
Sbjct: 12  VCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSD------------- 124
             L+ SLS+ L  YYP+AG+L D A+V+CND GA F    ++C MS+             
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 VTKNDEIWKLLCPGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWAS 184
           V   D  W   C G       +L VQ+++F CGG AI V  SHKI D  +   F+NDW+S
Sbjct: 132 VLPKDLPWANNCEGGN-----LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSS 191

Query: 185 IARNHRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVK 244
           + R+H      L  SP+F   S F            +    +    S L E      V K
Sbjct: 192 VTRDHT--TTTLVPSPRFVGDSVFST--------KTYGSLTTPQILSDLNE-----CVQK 251

Query: 245 RFAFESPKIAALKAMVSEK--VENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMV 304
           R  F + K+ AL+A V+E+  V+NPTR +++SAL++K A  ++       + PS L+  +
Sbjct: 252 RLIFPTDKLDALRAKVAEESGVKNPTRAEVVSALLFKCATKASSS-----MLPSKLVHFL 311

Query: 305 NIRKRVVPPLPEALKGNIITFFVVPL--QEEVELWEVVRDMKRNFEEFC--DKYPKN--- 364
           NIR  + P LP    GN+ + F +     +++EL  +VR++++  E     D+  +N   
Sbjct: 312 NIRTMIKPRLPRNTIGNLSSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELI 371

Query: 365 YEAVEWCSLHKLYMEESFGRMAQNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSI 424
            E VE     KL  E   G   +N Y CS+ C    Y VDFGWG+P  + + +   +N+ 
Sbjct: 372 LEVVESMREGKLPFENMDG--YENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAF 431

Query: 425 GLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSF 432
            L D K G+G+EA V L K+ M+ FE+N+ELL F
Sbjct: 432 FLKDYKAGQGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of MC08g1720 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.2e-57
Identity = 158/454 (34.80%), Postives = 231/454 (50.88%), Query Frame = 0

Query: 5   IVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFY----HNNESSAGGRRRSH-S 64
           + +K  IKPSS TP  LR    S  D  +   Y+ C FFY       E S      SH +
Sbjct: 12  VCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSD------------- 124
             L+ SLS+ L  YYP+AG+L D A+V+CND GA F    ++C MS+             
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 VTKNDEIWKLLCPGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWAS 184
           V   D  W   C G       +L VQ+++F CGG AI V  SHKI D  +   F+NDW+S
Sbjct: 132 VLPKDLPWANNCEGGN-----LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSS 191

Query: 185 IARNHRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVK 244
           + R+ R    L+P SP+F   S F   +    +        +    S L +      V K
Sbjct: 192 VTRD-RTTTTLVP-SPRFVGDSVFSTQKYGSLI--------TPQILSDLNQ-----CVQK 251

Query: 245 RFAFESPKIAALKAMVSEK--VENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMV 304
           R  F + K+ AL+A V+E+  V+NPTR +++SAL++K A  ++       + PS L+  +
Sbjct: 252 RLIFPTDKLDALRAKVAEESGVKNPTRAEVVSALLFKCATKASSS-----MLPSKLVHFL 311

Query: 305 NIRKRVVPPLPEALKGNIITFFVVPL--QEEVELWEVVRDMKRNFEEFC--DKYPKN--- 364
           NIR  + P LP    GN+ + F +     +++EL  +VR++++  E     D+  +N   
Sbjct: 312 NIRTMIKPRLPRNAIGNLSSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELI 371

Query: 365 YEAVEWCSLHKLYMEESFGRMAQNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSI 424
            E VE     KL  E   G   +N Y CS+ C    Y VDFGWG+P  + + +   +N+ 
Sbjct: 372 LEVVESMREGKLPFENMDG--YKNVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAF 431

Query: 425 GLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSF 432
            L D K G+G+EA V L K+ M+ FE+N+ELL F
Sbjct: 432 FLKDYKAGQGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of MC08g1720 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.4e-54
Identity = 144/440 (32.73%), Postives = 230/440 (52.27%), Query Frame = 0

Query: 2   EFEIVSKESIKPSSPTPPELR-HLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 61
           + +I+S+E I+PSSPTP  L+ H    L    + CH +  + FY  N+  +   R   SE
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCH-IPIILFY-PNQLDSNLDRAQRSE 63

Query: 62  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKN---DEIWKLL 121
            LK SLS VLT +YP AGR+   +SV+CND+G  F EA +   +S+  KN   DE+ + L
Sbjct: 64  NLKRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYL 123

Query: 122 ----CPG---DGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARN 181
                PG    G + +  L+V+++ F+CGG AI V +SHKI+D ++ ATF+N W +  + 
Sbjct: 124 PFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQ- 183

Query: 182 HRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAF 241
                E   V P F+  S   P  ES P                L +   ++IV+KRF F
Sbjct: 184 ----EETDIVQPNFDLGSHHFPPMESIPA------------PEFLPD---ENIVMKRFVF 243

Query: 242 ESPKIAALKAMVSE---KVENPTRTQLLSALIYKAAVISTGGL--GKQPLPPSSLLQMVN 301
           +  K+ ALKA ++    +V+N +R Q++ A+I+K  +  T      K  L  +   Q VN
Sbjct: 244 DKEKLEALKAQLASSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAA---QAVN 303

Query: 302 IRKRVVPPLPEALKGNIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCS 361
           +R R+ PP P++  GNI T      +E+ +  ++V  +K +  +  D++ K         
Sbjct: 304 LRSRMNPPFPQSAMGNIATMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVK--------E 363

Query: 362 LHKLYMEESFGRMAQNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDG 421
           L K      +    Q  +  SSWC +G Y++DFGWGKP  +    +  +N + LMD ++ 
Sbjct: 364 LQKGVTYLDYEAEPQELFSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNE 410

Query: 422 EGMEAVVCLDKEVMAVFEKN 426
           +GMEA + + ++ M++   +
Sbjct: 424 DGMEAWISMAEDEMSMLSSD 410

BLAST of MC08g1720 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.5e-53
Identity = 147/471 (31.21%), Postives = 237/471 (50.32%), Query Frame = 0

Query: 4   EIVSKESIKPSSPTPPELRHLSPSLFDAPIPC-HYVECVFFYHNNESSAGGRRRSHS--E 63
           E++SKE+IKP++PTP +L++ + SL D   P  +YV  + FY    +++ G    H   +
Sbjct: 10  EVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDLD 69

Query: 64  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSD-VTKNDEIWKLLCP 123
            LK SLS+ L H+YP AGR+ID   V+C+D G  F +  +R  M + +++ D     L P
Sbjct: 70  LLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQPDVPLSQLLP 129

Query: 124 GDGTEIN----PILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHH 183
            +    +     ++ VQ+  F CGG AIC  +SHKI+D  T +TF+  WAS  +  R   
Sbjct: 130 SEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTSRSGG 189

Query: 184 ELLPVS-----PQFNASSFFPPG-RESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFA 243
               V+     P F+++S FPP  R + P G++        FSS   +   D  V KRF 
Sbjct: 190 STAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEI-----PFSSTPEDTEDDKTVSKRFV 249

Query: 244 FESPKIAALKAMV------SEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQM 303
           F+  KI +++  +      + K    TR +++++LI+K+ + ST   G  P+    +   
Sbjct: 250 FDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVMKSTPA-GFLPV----VHHA 309

Query: 304 VNIRKRVVPPLPEALKGNII--------------------TFFVVPLQEEV---ELWEVV 363
           VN+RK++ PPL +   GN+                     T      + +V   EL + +
Sbjct: 310 VNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHELHDFI 369

Query: 364 RDMKRNFEEF------CDKYPKNYEAVEWCSLHKLYMEESFGRMAQNFYLCSSWCNVGLY 423
             M+   ++        +K  +N+ +    S+ K+   E       NF++ SSWC +GLY
Sbjct: 370 AQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDVEVI-----NFWI-SSWCRMGLY 429

BLAST of MC08g1720 vs. NCBI nr
Match: XP_022143303.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 858 bits (2218), Expect = 2.55e-314
Identity = 420/436 (96.33%), Postives = 421/436 (96.56%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 60
           MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE
Sbjct: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 60

Query: 61  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCPG 120
           ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCPG
Sbjct: 61  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCPG 120

Query: 121 DGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLPV 180
           DGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLPV
Sbjct: 121 DGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLPV 180

Query: 181 SPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAFESPKIAALKA 240
           SPQFNASSFFPPGRESCPL               +REQRRDHIVVKRFAFESPKIAALKA
Sbjct: 181 SPQFNASSFFPPGRESCPL---------------VREQRRDHIVVKRFAFESPKIAALKA 240

Query: 241 MVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALKG 300
           MVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALKG
Sbjct: 241 MVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALKG 300

Query: 301 NIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMAQ 360
           NIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMAQ
Sbjct: 301 NIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMAQ 360

Query: 361 NFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDGEGMEAVVCLDKEVMA 420
           NFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDGEGMEAVVCLDKEVMA
Sbjct: 361 NFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDGEGMEAVVCLDKEVMA 420

Query: 421 VFEKNQELLSFGCLKS 436
           VFEKNQELLSFGCLKS
Sbjct: 421 VFEKNQELLSFGCLKS 421

BLAST of MC08g1720 vs. NCBI nr
Match: XP_022143301.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 701 bits (1809), Expect = 5.21e-252
Identity = 349/438 (79.68%), Postives = 379/438 (86.53%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNN-ESSAGGRRRSHS 60
           MEFEIV+KESI+PSSPTPPELRHL  S FDA +PC Y++CVFFYHNN + SA  RRRSHS
Sbjct: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60

Query: 61  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCP 120
           E LK SLSEVLTHYYPFAGRLIDGASVECN+ GATFTEANL+CPMSDVTK+DEIWKLLCP
Sbjct: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120

Query: 121 GDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLP 180
            DGTEINPILSVQLTRFQCGGEAICVFLSHK+SD++TFATFMNDWASIAR+      LLP
Sbjct: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGA---LLP 180

Query: 181 VSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAFESPKIAALK 240
           VSPQFNA+SFFPPGR+  PL               +REQR D+ VVKRF FE  KI ALK
Sbjct: 181 VSPQFNAASFFPPGRKPFPL---------------VREQRVDNTVVKRFVFEGSKITALK 240

Query: 241 AMVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALK 300
            MVSEKVENPTR +LLSALIYKA VIS GG  K+P PPSSLLQ+VNIRKRVVPPLPEALK
Sbjct: 241 VMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALK 300

Query: 301 GNIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMA 360
           GNIITF+VVPLQEEVELWEVVRDMKRNFEEFCDKYP+NYEA EW SLHKL+MEESFGRMA
Sbjct: 301 GNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEESFGRMA 360

Query: 361 QNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAK-DGEGMEAVVCLDKEV 420
           QNFY CSSWCNVGLYEVDFG GKPAWITMPDI+R+NSI LMD K + EGMEA+VCLDK+V
Sbjct: 361 QNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDV 420

Query: 421 MAVFEKNQELLSFGCLKS 436
           MA FE+N+ELL FGCLKS
Sbjct: 421 MAAFERNEELLCFGCLKS 420

BLAST of MC08g1720 vs. NCBI nr
Match: KAG6581251.1 (Tabersonine-19-hydroxy-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 414 bits (1064), Expect = 1.93e-138
Identity = 241/462 (52.16%), Postives = 303/462 (65.58%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNE---SSAGGRRRS 60
           ME EIVSKE+IKP S TPP+L  L+ S FD   P  Y   ++FY NN    SS+G R R 
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRGRL 60

Query: 61  HSEELKESLSEVLTHYYPFAGRLIDGAS-VECNDAGATFTEANLRCPMSDVTK-----ND 120
               LK+SLS+ LT YYPFAGRL DG + ++CND GATF EA LRCPMS+V       +D
Sbjct: 61  ----LKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHD 120

Query: 121 EIWKLLC----PGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASI 180
           EI KLLC     G     +P+LSVQLT+F+CGGE + V L+HK++DL TFA FMNDWASI
Sbjct: 121 EILKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASI 180

Query: 181 ARNHRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKR 240
           AR+  G    + V P F+A+S F P  +    G++ S    +       ++R +++  +R
Sbjct: 181 ARSSGGIDPPV-VCPLFHAASLFRPELDG---GVDPSEGEGSG-----SKKREENVRSRR 240

Query: 241 FAFESPKIAALKAMVSEKVENPTRTQLLSALIYKAAVIS----TGGLGKQPLPPSSLLQM 300
             FE  KI ALKAMVS+KVENPTR Q+L+A IYKA V +    +GGL ++P   +S+LQ 
Sbjct: 241 MVFEGSKIRALKAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREP---TSILQF 300

Query: 301 VNIRKRVVPPLPEALKGNIITFF-----VVPLQEEVELWEVVRDMKRNFEEFCDKYPKNY 360
           +N+R RV PPLP  L GNI++FF         Q E+ELW VV DMKRNFEEFC K P+NY
Sbjct: 301 INLRSRVEPPLPGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNY 360

Query: 361 EAVEWCSLHKLYMEESFGR-MAQNF---YLCSSWCNVGLYEVDFGWGKPAWITMPDIIRR 420
              E  S  K +++ESF   M  N    Y CSSWC   LYE DFGWG P WI +P  + +
Sbjct: 361 RDEEGSSALKSWIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSK 420

Query: 421 NSIGLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSFGCLKS 436
           N+IGLMDAKDGEG+EA+VCLDK+ MAVFE N+ELLSF  LK+
Sbjct: 421 NTIGLMDAKDGEGIEAIVCLDKDEMAVFETNEELLSFCHLKT 446

BLAST of MC08g1720 vs. NCBI nr
Match: XP_022935661.1 (vinorine synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 412 bits (1059), Expect = 1.10e-137
Identity = 240/462 (51.95%), Postives = 302/462 (65.37%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNE---SSAGGRRRS 60
           ME EIVSKE+IKP S TPP+L  L+ S FD   P  Y   ++FY NN    SS+G R R 
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRGRL 60

Query: 61  HSEELKESLSEVLTHYYPFAGRLIDGAS-VECNDAGATFTEANLRCPMSDVTK-----ND 120
               LK+SLS+ LT YYPFAGRL DG + ++CND GATF EA LRCPMS+V       +D
Sbjct: 61  ----LKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHD 120

Query: 121 EIWKLLC----PGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASI 180
           EI KLLC     G     +P+LSVQLT+F+CGGE + V L+HK++DL TFA FMNDWASI
Sbjct: 121 EILKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASI 180

Query: 181 ARNHRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKR 240
           AR+  G    + V P F+A+S F P  +    G++ S    +       ++R +++  +R
Sbjct: 181 ARSSGGIDPPV-VCPLFHAASLFRPELDG---GVDPSEGEGSG-----SKKREENVRSRR 240

Query: 241 FAFESPKIAALKAMVSEKVENPTRTQLLSALIYKAAVIS----TGGLGKQPLPPSSLLQM 300
             FE  KI ALKAMVS+KVENPTR Q+L+A IYKA V +    +GGL ++P   +S+LQ 
Sbjct: 241 MVFEGSKIRALKAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREP---TSILQF 300

Query: 301 VNIRKRVVPPLPEALKGNIITFF-----VVPLQEEVELWEVVRDMKRNFEEFCDKYPKNY 360
           +N+R RV PPLP  L GNI++FF         Q E+ELW VV DMKRNFEEFC K P+NY
Sbjct: 301 INLRSRVEPPLPGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNY 360

Query: 361 EAVEWCSLHKLYMEESFGR-MAQNF---YLCSSWCNVGLYEVDFGWGKPAWITMPDIIRR 420
              E     K +++ESF   M  N    Y CSSWC   LYE DFGWG P WI +P  + +
Sbjct: 361 RDEEGSPALKSWIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSK 420

Query: 421 NSIGLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSFGCLKS 436
           N+IGLMDAKDGEG+EA+VCLDK+ MAVFE N+ELLSF  LK+
Sbjct: 421 NTIGLMDAKDGEGIEAIVCLDKDEMAVFETNEELLSFCHLKT 446

BLAST of MC08g1720 vs. NCBI nr
Match: XP_023528950.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 411 bits (1057), Expect = 2.21e-137
Identity = 242/462 (52.38%), Postives = 301/462 (65.15%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNE---SSAGGRRRS 60
           ME EIVSKE+IKP S TPP+L  L+ S FD   P  Y   ++FY NN    SS+G R R 
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRGRL 60

Query: 61  HSEELKESLSEVLTHYYPFAGRLIDGAS-VECNDAGATFTEANLRCPMSDVTK-----ND 120
               LK+SLS+ LT YYPFAGRL DG + ++CND GATF EA LRCPMS+V       +D
Sbjct: 61  ----LKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFLEAKLRCPMSEVMNTFDLHHD 120

Query: 121 EIWKLLC----PGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASI 180
           EI KLLC     G+    +P+LSVQLT+F+CGGE + V L+HK++DL TFA FMNDWASI
Sbjct: 121 EILKLLCFEDIKGNVQTFDPLLSVQLTQFECGGEIMYVSLAHKVADLATFANFMNDWASI 180

Query: 181 ARNHRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKR 240
           AR+  G    + V P F+A+S F P        LN     S    S  ++ R +++  +R
Sbjct: 181 ARSSGGSDPPV-VCPLFHAASLFRPE-------LNGGGDPSEGEGSGSKK-REENVRSRR 240

Query: 241 FAFESPKIAALKAMVSEKVENPTRTQLLSALIYKAAVIS----TGGLGKQPLPPSSLLQM 300
             FE  KI ALKAMVS+KVENPTR Q+L+A IYKA V +    +GG  ++P   +S+LQ 
Sbjct: 241 MVFEGSKINALKAMVSKKVENPTRVQVLTAFIYKAMVSTKNALSGGFSREP---TSILQF 300

Query: 301 VNIRKRVVPPLPEALKGNIITFF-----VVPLQEEVELWEVVRDMKRNFEEFCDKYPKNY 360
           +N+R RV PPLP  L GNI++FF         Q E+ELW VV DMKRNFEEFC K P+NY
Sbjct: 301 INLRSRVEPPLPGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNY 360

Query: 361 EAVEWCSLHKLYMEESFGR-MAQNF---YLCSSWCNVGLYEVDFGWGKPAWITMPDIIRR 420
              E  S  K +++ESF   M  N    Y CSSWC   LYE DFGWG P WI +P  + +
Sbjct: 361 RDEEGSSGLKSWIKESFETWMIDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSK 420

Query: 421 NSIGLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSFGCLKS 436
           N+IGLMDAKDGEG+EA+VCLDK+ MAVFE N+ELLSF  LK+
Sbjct: 421 NTIGLMDAKDGEGIEAIVCLDKDEMAVFETNEELLSFCHLKT 446

BLAST of MC08g1720 vs. ExPASy TrEMBL
Match: A0A6J1CQD5 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111013207 PE=3 SV=1)

HSP 1 Score: 858 bits (2218), Expect = 1.24e-314
Identity = 420/436 (96.33%), Postives = 421/436 (96.56%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 60
           MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE
Sbjct: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 60

Query: 61  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCPG 120
           ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCPG
Sbjct: 61  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCPG 120

Query: 121 DGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLPV 180
           DGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLPV
Sbjct: 121 DGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLPV 180

Query: 181 SPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAFESPKIAALKA 240
           SPQFNASSFFPPGRESCPL               +REQRRDHIVVKRFAFESPKIAALKA
Sbjct: 181 SPQFNASSFFPPGRESCPL---------------VREQRRDHIVVKRFAFESPKIAALKA 240

Query: 241 MVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALKG 300
           MVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALKG
Sbjct: 241 MVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALKG 300

Query: 301 NIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMAQ 360
           NIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMAQ
Sbjct: 301 NIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMAQ 360

Query: 361 NFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDGEGMEAVVCLDKEVMA 420
           NFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDGEGMEAVVCLDKEVMA
Sbjct: 361 NFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDGEGMEAVVCLDKEVMA 420

Query: 421 VFEKNQELLSFGCLKS 436
           VFEKNQELLSFGCLKS
Sbjct: 421 VFEKNQELLSFGCLKS 421

BLAST of MC08g1720 vs. ExPASy TrEMBL
Match: A0A6J1CPW0 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111013206 PE=3 SV=1)

HSP 1 Score: 701 bits (1809), Expect = 2.52e-252
Identity = 349/438 (79.68%), Postives = 379/438 (86.53%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNN-ESSAGGRRRSHS 60
           MEFEIV+KESI+PSSPTPPELRHL  S FDA +PC Y++CVFFYHNN + SA  RRRSHS
Sbjct: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60

Query: 61  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCP 120
           E LK SLSEVLTHYYPFAGRLIDGASVECN+ GATFTEANL+CPMSDVTK+DEIWKLLCP
Sbjct: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120

Query: 121 GDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLP 180
            DGTEINPILSVQLTRFQCGGEAICVFLSHK+SD++TFATFMNDWASIAR+      LLP
Sbjct: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGA---LLP 180

Query: 181 VSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAFESPKIAALK 240
           VSPQFNA+SFFPPGR+  PL               +REQR D+ VVKRF FE  KI ALK
Sbjct: 181 VSPQFNAASFFPPGRKPFPL---------------VREQRVDNTVVKRFVFEGSKITALK 240

Query: 241 AMVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALK 300
            MVSEKVENPTR +LLSALIYKA VIS GG  K+P PPSSLLQ+VNIRKRVVPPLPEALK
Sbjct: 241 VMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALK 300

Query: 301 GNIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMA 360
           GNIITF+VVPLQEEVELWEVVRDMKRNFEEFCDKYP+NYEA EW SLHKL+MEESFGRMA
Sbjct: 301 GNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEESFGRMA 360

Query: 361 QNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAK-DGEGMEAVVCLDKEV 420
           QNFY CSSWCNVGLYEVDFG GKPAWITMPDI+R+NSI LMD K + EGMEA+VCLDK+V
Sbjct: 361 QNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDV 420

Query: 421 MAVFEKNQELLSFGCLKS 436
           MA FE+N+ELL FGCLKS
Sbjct: 421 MAAFERNEELLCFGCLKS 420

BLAST of MC08g1720 vs. ExPASy TrEMBL
Match: A0A6J1F5C1 (vinorine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442451 PE=3 SV=1)

HSP 1 Score: 412 bits (1059), Expect = 5.34e-138
Identity = 240/462 (51.95%), Postives = 302/462 (65.37%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNE---SSAGGRRRS 60
           ME EIVSKE+IKP S TPP+L  L+ S FD   P  Y   ++FY NN    SS+G R R 
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRGRL 60

Query: 61  HSEELKESLSEVLTHYYPFAGRLIDGAS-VECNDAGATFTEANLRCPMSDVTK-----ND 120
               LK+SLS+ LT YYPFAGRL DG + ++CND GATF EA LRCPMS+V       +D
Sbjct: 61  ----LKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHD 120

Query: 121 EIWKLLC----PGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASI 180
           EI KLLC     G     +P+LSVQLT+F+CGGE + V L+HK++DL TFA FMNDWASI
Sbjct: 121 EILKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASI 180

Query: 181 ARNHRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKR 240
           AR+  G    + V P F+A+S F P  +    G++ S    +       ++R +++  +R
Sbjct: 181 ARSSGGIDPPV-VCPLFHAASLFRPELDG---GVDPSEGEGSG-----SKKREENVRSRR 240

Query: 241 FAFESPKIAALKAMVSEKVENPTRTQLLSALIYKAAVIS----TGGLGKQPLPPSSLLQM 300
             FE  KI ALKAMVS+KVENPTR Q+L+A IYKA V +    +GGL ++P   +S+LQ 
Sbjct: 241 MVFEGSKIRALKAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREP---TSILQF 300

Query: 301 VNIRKRVVPPLPEALKGNIITFF-----VVPLQEEVELWEVVRDMKRNFEEFCDKYPKNY 360
           +N+R RV PPLP  L GNI++FF         Q E+ELW VV DMKRNFEEFC K P+NY
Sbjct: 301 INLRSRVEPPLPGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNY 360

Query: 361 EAVEWCSLHKLYMEESFGR-MAQNF---YLCSSWCNVGLYEVDFGWGKPAWITMPDIIRR 420
              E     K +++ESF   M  N    Y CSSWC   LYE DFGWG P WI +P  + +
Sbjct: 361 RDEEGSPALKSWIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSK 420

Query: 421 NSIGLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSFGCLKS 436
           N+IGLMDAKDGEG+EA+VCLDK+ MAVFE N+ELLSF  LK+
Sbjct: 421 NTIGLMDAKDGEGIEAIVCLDKDEMAVFETNEELLSFCHLKT 446

BLAST of MC08g1720 vs. ExPASy TrEMBL
Match: A0A0A0KV47 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001560 PE=3 SV=1)

HSP 1 Score: 399 bits (1024), Expect = 8.87e-133
Identity = 222/461 (48.16%), Postives = 291/461 (63.12%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 60
           M+ EIVSKE+IKP   TPPEL+     LFD   P  Y+  ++FY N+         SHS+
Sbjct: 2   MQLEIVSKETIKPLFSTPPELKLHHLCLFDRHAPYLYLHFLYFYQNS---------SHSK 61

Query: 61  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVT-----KNDEIWK 120
            LK SLS+ LT YYPFAGRL D  S++CND GAT  EA LRCPMS+       ++DE  K
Sbjct: 62  LLKHSLSKALTFYYPFAGRLKDDCSIDCNDMGATILEARLRCPMSEFMNVYNLRHDEALK 121

Query: 121 LLC---PGDGTE---INPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIAR 180
           L+C     DG++    NP+L VQLTRF+CGGE +CVFLSHK++D  TF  FMN WAS++R
Sbjct: 122 LVCFDDMNDGSKDQRYNPLLCVQLTRFECGGEVLCVFLSHKLADASTFTNFMNHWASLSR 181

Query: 181 NHRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFA 240
                  LLP  P+F+A S F        LG                 +R  ++V KR  
Sbjct: 182 TGDHDMPLLPP-PRFDAGSLFEAATVDDVLGEGSKI-----------VKREKNLVSKRMV 241

Query: 241 FESPKIAALKAMVSEKVENPTRTQLLSALIYKAAVISTGGL-----GKQPLPPSSLLQMV 300
           F++ KIAALKA V +KVENP+R Q+L+A IYKAA+ ++         + P+    L   +
Sbjct: 242 FKASKIAALKATVCDKVENPSRVQILAAFIYKAAISASKSTLSIHHSRTPITNYLLRIAI 301

Query: 301 NIRKRVVPPLPEALKGNIITFFVVPL-----QEEVELWEVVRDMKRNFEEFCDKYPKNYE 360
           N+R R++PP+P  L GNII+FF+        Q+E+EL  ++ DMKRN +EFC+KYP+NY+
Sbjct: 302 NLRTRLIPPMPATLSGNIISFFLTSATTTAEQKEIELSSLLSDMKRNMKEFCNKYPRNYK 361

Query: 361 AVEWCSLHKLYMEESFGRMA----QNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRN 420
           A EW SL+KL+++ES  RM     Q  Y CSSWC    Y+ DFGWGKP WIT P I+ +N
Sbjct: 362 AEEWSSLYKLHIKESMERMNNPEDQIVYACSSWCRFPFYDADFGWGKPVWITTPTILSKN 421

Query: 421 SIGLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSFGCLKS 436
            I LMDAKDGEG+EA V L++E MAVFE+N+ELLS+  L+S
Sbjct: 422 VIILMDAKDGEGIEAFVSLEEEEMAVFEQNEELLSYCELES 441

BLAST of MC08g1720 vs. ExPASy TrEMBL
Match: A0A6J1F342 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111441702 PE=3 SV=1)

HSP 1 Score: 395 bits (1014), Expect = 7.66e-131
Identity = 232/476 (48.74%), Postives = 299/476 (62.82%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNE---SSAGGRRRS 60
           ME EI+ KE+IKP + T PELR+L   LFD   P  Y++ ++FY NN    SSAG RRR 
Sbjct: 1   MELEILCKETIKPITSTSPELRNLPRCLFDQQAPNIYMQLLYFYTNNNAVVSSAGCRRRL 60

Query: 61  HSEELKESLSEVLTHYYPFAGRLIDGAS-VECNDAGATFTEANLRCPMSDVTKN-----D 120
               LK+SLS+ LT YYPFAGRL DG + ++CND GA F+EA LRC M +   N     +
Sbjct: 61  ----LKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGAIFSEAKLRCSMLEFMNNFNLERE 120

Query: 121 EIWKLLCP----GDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASI 180
           EI KL       G+    NP+L +QLT+F+CGG+ + VF SHKI+DL +   FMNDWASI
Sbjct: 121 EILKLTFAVDINGNDPTFNPLLLIQLTQFECGGDVMFVFCSHKIADLASLTNFMNDWASI 180

Query: 181 ARNHRGHHELLPVSPQFNASSFFPPGRESC--PLGLNHSCHRSNYFSSHLREQRRDHIVV 240
           AR+     +L  V+P+ N  S+FPP  ++   P G           S  +R  R +++  
Sbjct: 181 ARSS-DDGDLPVVNPEINGVSYFPPELDAGGDPFGGGDG-------SGTVR--RDENVCS 240

Query: 241 KRFAFESPKIAALKAMVSEKVENPTRTQLLSALIYKA---AVISTGGLGKQPL------- 300
           KR  FE  KIAALKAMVSE+VENPTR Q+L+A IYKA   A  S  G  K+ L       
Sbjct: 241 KRLVFEGSKIAALKAMVSEEVENPTRVQILTAFIYKAVLSAKFSATGYNKKILTFVCGAN 300

Query: 301 -------PPSSLLQMVNIRKRVVPPLPEALKGNIITFFVVPL----QEEVELWEVVRDMK 360
                  P +SLLQ++N+R+RV PPLPE L GNIITFF+       + E+EL  +V DMK
Sbjct: 301 LFYKGSRPATSLLQLINLRRRVEPPLPETLTGNIITFFIASWTAAEKREMELCNMVGDMK 360

Query: 361 RNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMA----QNFYLCSSWCNVGLYEVDFGW 420
            +FEEFC  +P+NY    W SLHKL+ +ES         ++ Y C+SWC   +YE DFGW
Sbjct: 361 TSFEEFCKTFPRNYRDEAWSSLHKLHAKESMETWRNPGERSVYTCTSWCRFPMYEADFGW 420

Query: 421 GKPAWITMPDIIRRNSIGLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSFGCLKS 436
           GKP WIT+P+   +N I LMDAK+G+G+EA+V LDKE MA FE+NQELLSF  LK+
Sbjct: 421 GKPEWITVPEAPWKNMILLMDAKNGDGIEAMVSLDKEEMAAFEQNQELLSFCELKT 462

BLAST of MC08g1720 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 240.0 bits (611), Expect = 3.7e-63
Identity = 162/456 (35.53%), Postives = 243/456 (53.29%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 60
           M  ++VS++ IKPSSPTP  L+    SL +   P  +   VFFY  N S    +     +
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSI---KPTEQLQ 60

Query: 61  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDV---TKNDEIWKLL 120
            LK+SLSE LTH+YP AGRL    S++CND+GA F EA +  P+S++     +D + +L+
Sbjct: 61  MLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLI 120

Query: 121 CPG-DGTEINP-ILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHH 180
               D  E    +L  Q + F+CG  +I V +SHK++D  +   FM  WA+I  + RG  
Sbjct: 121 PTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAI--SSRGSI 180

Query: 181 ELLPVSPQFNASSFFPPGR--ESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAFESP 240
           + +  +P F+    FPPG   E+ P  +               E   +  + KRF F+S 
Sbjct: 181 KTIG-APVFDTVKIFPPGNFSETSPAPVVEP------------EIMMNQTLSKRFIFDSS 240

Query: 241 KIAALKAMVSE-KVENPTRTQLLSALIYKAAVIST---GGLGKQPLPPSSLLQMVNIRKR 300
            I AL+A  S  +V  PTR + +SALI+K+A+ +T    G  K    PS L   V++R R
Sbjct: 241 SIQALQAKASSFEVNQPTRVEAVSALIWKSAMKATRTVSGTSK----PSILANSVSLRSR 300

Query: 301 VVPPLPEALKGNIITFFVVPLQEEVE------LWEVVRDMKRNFEEF-CDKYPKNYEAVE 360
           V PP  +   GN++++F    +E +       L   +R  K+ F +    K   N  A E
Sbjct: 301 VSPPFTKNSIGNLVSYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATE 360

Query: 361 -WCSLHKLYMEESFGRMAQ---NFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIG 420
             CS    Y +E+   +A    +FY+ SS C  GLYE DFGWGKP W+  P + ++N + 
Sbjct: 361 IICS----YQKEAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVT 420

Query: 421 LMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSFGCL 435
           L+D K+  G+EA V L+++ M +FE+++ELL F  L
Sbjct: 421 LLDTKEAGGIEAWVNLNEQEMNLFEQDRELLQFASL 430

BLAST of MC08g1720 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 220.3 bits (560), Expect = 3.0e-57
Identity = 155/457 (33.92%), Postives = 234/457 (51.20%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 60
           M+ E +SKE IKPSSPTP  L+ L  S++D  +P  Y     FY  N+  +   +   S 
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLIS---QEHTSH 65

Query: 61  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANL-RCPMSDVTK---NDEIWKL 120
           +LK SLSE LT +YP AGR I G +V+C D GA F +A +  CP+++  K    D + +L
Sbjct: 66  KLKTSLSETLTKFYPLAGR-ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQL 125

Query: 121 L------CPGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARN 180
           L       P       P+L V+ T F CGG AI + ++HKI+D  + +TF+  WA+ AR 
Sbjct: 126 LPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARG 185

Query: 181 HRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAF 240
                 +   SP F  ++F+PP  E+  L  +    + +              + KRF F
Sbjct: 186 ENDAAAM--ESPVFAGANFYPPANEAFKLPADEQAGKRS-------------SITKRFVF 245

Query: 241 ESPKIAAL--KAMVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRK 300
           E+ K+  L  KA   E V+ PTR + ++ALI+K  V S+            L+Q+ N+R 
Sbjct: 246 EASKVEDLRTKAASEETVDQPTRVESVTALIWKCFVASS---KTTTCDHKVLVQLANLRS 305

Query: 301 RVVPPLPEALKGNIITFFVV---PLQEEVELWEVVRDMKRNFEE----FCDKYPKNYEAV 360
           ++   L E+  GN++   VV       EV++ E VRD+++  EE      D+   +  + 
Sbjct: 306 KIPSLLQESSIGNLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSS 365

Query: 361 EWCSLHKLYMEESFGRMAQNF---YLCSSWCNVGLYEVDFGWGKPAWIT-MPDIIRRNSI 420
              S     M  ++ R++      Y  SSWC + LYE  FGW  P W+      +  N  
Sbjct: 366 MIGSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLA 425

Query: 421 GLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSFGCL 435
            L+D+KDG+G+EA V L +E M+ FE+N ELL+F  +
Sbjct: 426 MLIDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATM 440

BLAST of MC08g1720 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 213.8 bits (543), Expect = 2.8e-55
Identity = 148/457 (32.39%), Postives = 233/457 (50.98%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNNESSAGGRRRSHSE 60
           M+ E ++KE IKPSS TP +L+ L  S++D  +P  Y     FY  ++  +       S 
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLIS---PEQSSH 63

Query: 61  ELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANL-RCPMSDVTKNDE---IWKL 120
           +LK SL+E LT +YP AGR I G +++CND GA F +A +   P+SD  ++ +   + +L
Sbjct: 64  KLKTSLAETLTKFYPLAGR-IKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQL 123

Query: 121 L------CPGDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARN 180
           L       P +     P+L V+ T F CGG AI + ++HKI+D  + +TF+  WA++AR 
Sbjct: 124 LPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARG 183

Query: 181 HRGHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAF 240
             G    +   P F A++F+PP  E     ++ + ++ +              + KRF F
Sbjct: 184 EAGD---IVADPVFAAANFYPPANELFKFPVDENANKISQ-------------ITKRFVF 243

Query: 241 ESPKIAAL--KAMVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRK 300
            + K+  L  KA   + V  P R + ++AL++KA V +             L+Q  N+R 
Sbjct: 244 SASKLEELRTKAASEDFVGRPKRVESVTALLWKAFVAAASS-NTNACDMKVLIQPSNLRP 303

Query: 301 RVVPPLPEALKGNIITFFVV---PLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCS 360
           ++   L E+L GNI+   VV     QEE+++ + VRD+++  ++      +N    E  S
Sbjct: 304 KIPSLLAESLIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDL-----QNVIKEEGGS 363

Query: 361 LHKLYMEESFGRMAQNF----------YLCSSWCNVGLYEVDFGWGKPAWIT-MPDIIRR 420
              +   +    M  N+          Y  SSWC + LYE  FGWG P WI      +  
Sbjct: 364 SSAMIGSKLANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLE 423

Query: 421 NSIGLMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSF 432
           N   L+D+KDG+G+EA V L +E M   E+N ELL+F
Sbjct: 424 NVTMLIDSKDGQGIEAFVTLPEENMLSLEQNTELLAF 434

BLAST of MC08g1720 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.6e-53
Identity = 156/451 (34.59%), Postives = 235/451 (52.11%), Query Frame = 0

Query: 4   EIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFY-HNNESSAGGRRRSHSEEL 63
           EI+S+E +KPSSPTP + R L+ SL D      Y   + FY  + ++  G      S +L
Sbjct: 6   EILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLKL 65

Query: 64  KESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTK--NDEIWKLLCPG 123
           K+SLS+ L  +YP AGR+I G+ VECND GA F EA +   +S+  K    E  +LL P 
Sbjct: 66  KKSLSKTLPIFYPLAGRII-GSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLELLIPV 125

Query: 124 DGTEIN----PILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHE 183
           +         P+L +Q   F CGG  I + +SHKI+D  + A F+  WA  +R       
Sbjct: 126 EAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRGLG---- 185

Query: 184 LLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAFESPKIA 243
            + + P F AS  FP   +  P          +       E      V KRF F++ KI 
Sbjct: 186 -ITLIPSFTASEVFPKPLDELP----------SKPMDRKEEVEEMSCVTKRFVFDASKIK 245

Query: 244 ALKAMVSEK-VENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQ-MVNIRKRVVPPL 303
            L+A  S   V+NPTR + ++AL ++  V     L     P +S+LQ +VN+R + V  L
Sbjct: 246 KLRAKASRNLVKNPTRVEAVTALFWR-CVTKVSRLSSL-TPRTSVLQILVNLRGK-VDSL 305

Query: 304 PEALKGNIITFFVVPLQEEV--ELWEVVRDMKRNFEEF---CDKYPKN----YEAVEWCS 363
            E   GN+++  ++  +E     + +VV +++R  E F   C +  K+    +E +E   
Sbjct: 306 CENTIGNMLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFELLE--E 365

Query: 364 LHKLYMEESFGR-MAQNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIR-RNSIGLMDAK 423
           + K+Y     GR    + ++ +SWC +GLY+ DFGWGKP W+T       +N + L+D K
Sbjct: 366 IGKVY-----GRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTK 425

Query: 424 DGEGMEAVVCLDKEVMAVFEKNQELLSFGCL 435
           DGEG+EA + L +E M++FE +QELL    L
Sbjct: 426 DGEGIEAWITLTEEQMSLFECDQELLESASL 430

BLAST of MC08g1720 vs. TAIR 10
Match: AT5G23970.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 198.7 bits (504), Expect = 9.4e-51
Identity = 149/453 (32.89%), Postives = 224/453 (49.45%), Query Frame = 0

Query: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFY--HNNESSAGGRRRSH 60
           M+ E++SKE IKP+SP    L+ LS SLFD  +P  YV  +FFY  H+N+          
Sbjct: 1   MKLELLSKEVIKPASPN--HLQTLSLSLFDQFLPSTYVSAIFFYDDHSNQEDI------- 60

Query: 61  SEELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKN----DEIW 120
            + LK SLS+ L+ +YP AG++ DG +V CND GA FTEA     +SD  +N    D I 
Sbjct: 61  -QRLKSSLSQTLSLFYPLAGQIKDGVTVHCNDEGALFTEARAEIFLSDFLRNPSDADLIQ 120

Query: 121 KLLCPGD--GTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHR 180
           K +   D    E  P+L V++  F+  G A+ V +SHKI D  + +TF+  W   ++ + 
Sbjct: 121 KFIVSPDHADPETWPLLHVKVIFFKDKGFAVAVSVSHKICDAASLSTFVCSWTKASKGYA 180

Query: 181 GHHELLPVSPQFNASSFFPPGRESCPLGLNHSCHRSNYFSSHLREQRRDHIVVKRFAFES 240
                  V P+F  + F+PP   S              F   L  + +     KRF F S
Sbjct: 181 D-----TVDPEFVGADFYPPADISIE------------FPPLLVHETKSK--TKRFVFGS 240

Query: 241 PKIAALK--AMVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRV 300
             I  LK  A   ++V   TR + ++AL+ +  +   G      +   ++ Q +++R RV
Sbjct: 241 LMIEKLKNRASSGKRVPQATRIESITALLLR-CMTKAGHSKSGKVKEFAITQTMDLRPRV 300

Query: 301 VPP-LPEALKGNIITFFVVPLQEE-----VELWEVVRDMKRNFEEFCDKYPKNYE----A 360
               LP    GN   FF +PL +E     +E+ E V  +++  +E  +    + E    +
Sbjct: 301 SSSLLPHKAIGN---FFFLPLLKESSESKMEIEETVSKLQKTKQELNELIRNDSEDAKSS 360

Query: 361 VEWCSLHKLYMEESFGRMAQNF--YLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIG 420
           VE        M  S   ++     Y  SSWC +  YE +FGWGKP W+  PD + +  + 
Sbjct: 361 VEAKERIASAMLSSLCEISPEMETYAVSSWCRMSFYEANFGWGKPVWVA-PDSVDKTQVV 419

Query: 421 LMDAKDGEGMEAVVCLDKEVMAVFEKNQELLSF 432
           LMD+KD EG+EA V L +  MA +E + ELL +
Sbjct: 421 LMDSKDSEGVEARVTLPETDMAKYEHDSELLVY 419