MC08g1719 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1719 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.6e-55
Identity = 159/457 (34.79%), Postives = 237/457 (51.86%), Query Frame = 0

Query: 5   IVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRR---SH-S 64
           I +K  I+PSS TP  LR   LSF D S+   Y+ C FFY    Q   DS+     SH +
Sbjct: 12  ICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV---TKSDEIWKL 124
            +L+ SLS+ L  YYP+AG+L D A+V+CN++GA F    ++C MS++     +     +
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 LCPED-----GTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIAR-- 184
           + P+D       E   +L VQ+++F CGG AI V  SHK+ D  +   F+NDW+S+ R  
Sbjct: 132 VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTRDH 191

Query: 185 SPGALLPVSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTV 244
           +   L+P SP+F   S F           + K   + I P  L            ++  V
Sbjct: 192 TTTTLVP-SPRFVGDSVF-----------STKKYGSLITPQIL----------SDLNECV 251

Query: 245 VKRFVFEGSKITALKVMVSEK--VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQ 304
            KR +F   K+ AL+  V+E+  V+NPTR E++SAL++K    ++         PS L+ 
Sbjct: 252 QKRLIFPTDKLDALRAKVAEESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVH 311

Query: 305 VVNIRKRVVPPLPEALKGNIITFYVVPL--QEEVELWEVVRDMKRNFEEFC--DKYPQN- 364
            +NIR  + P LP    GN+ + + +     +++EL  +VR++++  E     D+  QN 
Sbjct: 312 FLNIRTMIKPRLPRNAIGNLSSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNE 371

Query: 365 --YEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKN 424
              E  E +   KL  E   G   +N YTCS+ C    Y VDFG G+P  + + +   KN
Sbjct: 372 LILEVVESMREGKLPFENMDG--YENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKN 431

Query: 425 SIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 439
           +  L D K   +G+EA V L K  M+ FERNEELL F
Sbjct: 432 AFFLKDYKAG-QGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of MC08g1719 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.1e-55
Identity = 158/457 (34.57%), Postives = 237/457 (51.86%), Query Frame = 0

Query: 5   IVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRR---SH-S 64
           + NK  I+PSS TP  LR   LSF D S+   Y+ C FFY    Q   DS+     SH +
Sbjct: 12  VCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV---TKSDEIWKL 124
            +L+ SLS+ L  YYP+AG+L D A+V+CN++GA F    ++C MS++     +     +
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 LCPED-----GTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIA--R 184
           + P+D       E   +L VQ+++F CGG AI V  SHK+ D  +   F+NDW+S+   R
Sbjct: 132 VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTRDR 191

Query: 185 SPGALLPVSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTV 244
           +   L+P SP+F   S F           + +   + I P  L            ++  V
Sbjct: 192 TTTTLVP-SPRFVGDSVF-----------STQKYGSLITPQIL----------SDLNQCV 251

Query: 245 VKRFVFEGSKITALKVMVSEK--VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQ 304
            KR +F   K+ AL+  V+E+  V+NPTR E++SAL++K    ++         PS L+ 
Sbjct: 252 QKRLIFPTDKLDALRAKVAEESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVH 311

Query: 305 VVNIRKRVVPPLPEALKGNIITFYVVPL--QEEVELWEVVRDMKRNFEEFC--DKYPQN- 364
            +NIR  + P LP    GN+ + + +     +++EL  +VR++++  E     D+  QN 
Sbjct: 312 FLNIRTMIKPRLPRNAIGNLSSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNE 371

Query: 365 --YEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKN 424
              E  E +   KL  E   G   +N YTCS+ C    Y VDFG G+P  + + +   KN
Sbjct: 372 LILEVVESMREGKLPFENMDG--YKNVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKN 431

Query: 425 SIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 439
           +  L D K   +G+EA V L K  M+ FERNEELL F
Sbjct: 432 AFFLKDYKAG-QGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of MC08g1719 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 6.2e-55
Identity = 157/457 (34.35%), Postives = 236/457 (51.64%), Query Frame = 0

Query: 5   IVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRR---SH-S 64
           + +K  I+PSS TP  LR   LSF D S+   Y+ C FFY    Q   DS+     SH +
Sbjct: 12  VCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV---TKSDEIWKL 124
            +L+ SLS+ L  YYP+AG+L D A+V+CN++GA F    ++C MS++     +     +
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 LCPED-----GTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIAR-- 184
           + P+D       E   +L VQ+++F CGG AI V  SHK+ D  +   F+NDW+S+ R  
Sbjct: 132 VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTRDH 191

Query: 185 SPGALLPVSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTV 244
           +   L+P SP+F   S F           + K   +   P  L            ++  V
Sbjct: 192 TTTTLVP-SPRFVGDSVF-----------STKTYGSLTTPQIL----------SDLNECV 251

Query: 245 VKRFVFEGSKITALKVMVSEK--VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQ 304
            KR +F   K+ AL+  V+E+  V+NPTR E++SAL++K    ++         PS L+ 
Sbjct: 252 QKRLIFPTDKLDALRAKVAEESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVH 311

Query: 305 VVNIRKRVVPPLPEALKGNIITFYVVPL--QEEVELWEVVRDMKRNFEEFC--DKYPQN- 364
            +NIR  + P LP    GN+ + + +     +++EL  +VR++++  E     D+  QN 
Sbjct: 312 FLNIRTMIKPRLPRNTIGNLSSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNE 371

Query: 365 --YEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKN 424
              E  E +   KL  E   G   +N YTCS+ C    Y VDFG G+P  + + +   KN
Sbjct: 372 LILEVVESMREGKLPFENMDG--YENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKN 431

Query: 425 SIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 439
           +  L D K   +G+EA V L K  M+ FERNEELL F
Sbjct: 432 AFFLKDYKAG-QGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of MC08g1719 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 6.4e-52
Identity = 136/440 (30.91%), Postives = 231/440 (52.50%), Query Frame = 0

Query: 2   EFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHSE 61
           + +I+++E I+PSSPTP  L+   LS  D  +   ++  + FY   NQ  ++  R   SE
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFY--PNQLDSNLDRAQRSE 63

Query: 62  VLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKS---DEIWKLL 121
            LK SLS VLT +YP AGR+   +SV+CN+ G  F EA +   +S+  K+   DE+ + L
Sbjct: 64  NLKRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYL 123

Query: 122 -------CPEDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARS 181
                    E G + +  L+V+++ F+CGG AI V +SHK++D ++ ATF+N W +  + 
Sbjct: 124 PFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQE 183

Query: 182 PGALLPVSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTVV 241
              +  V P F+  S   P  +  P        A   LP                +N V+
Sbjct: 184 ETDI--VQPNFDLGSHHFPPMESIP--------APEFLP---------------DENIVM 243

Query: 242 KRFVFEGSKITALKVMVSE---KVENPTRIELLSALIYKAVV-ISAGGFEKRPPPPSSLL 301
           KRFVF+  K+ ALK  ++    +V+N +R++++ A+I+K  + ++   F+ +    ++  
Sbjct: 244 KRFVFDKEKLEALKAQLASSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAA-- 303

Query: 302 QVVNIRKRVVPPLPEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAA 361
           Q VN+R R+ PP P++  GNI T      +E+ +  ++V  +K +  +  D++ +     
Sbjct: 304 QAVNLRSRMNPPFPQSAMGNIATMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVK----- 363

Query: 362 EWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMD 421
               L K      +    Q  ++ SSWC +G Y++DFG GKP  +    +  KN + LMD
Sbjct: 364 ---ELQKGVTYLDYEAEPQELFSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMD 405

Query: 422 LKHELEGMEALVCLDKDVMA 428
            ++E +GMEA + + +D M+
Sbjct: 424 TRNE-DGMEAWISMAEDEMS 405

BLAST of MC08g1719 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 7.3e-48
Identity = 146/483 (30.23%), Postives = 237/483 (49.07%), Query Frame = 0

Query: 4   EIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS--E 63
           E+++KE+I+P++PTP +L++  LS  D   P  Y   +  ++     ++      H   +
Sbjct: 10  EVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDLD 69

Query: 64  VLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSD-VTKSDEIWKLLCP 123
           +LK SLS+ L H+YP AGR+ID   V+C++ G  F +  ++  M + +++ D     L P
Sbjct: 70  LLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQPDVPLSQLLP 129

Query: 124 EDGTEIN----PILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWAS---IARSPG 183
            +    +     ++ VQ+  F CGG AIC  +SHK++D  T +TF+  WAS    +RS G
Sbjct: 130 SEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTSRSGG 189

Query: 184 ALLPVS-----PQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDN 243
           +   V+     P F++AS FPP       ER         +PF+          +   D 
Sbjct: 190 STAAVTDQKLIPSFDSASLFPPS------ERLTSPSGMSEIPFS------STPEDTEDDK 249

Query: 244 TVVKRFVFEGSKITA----LKVMVSE--KVENPTRIELLSALIYKAVVISAGGFEKRPPP 303
           TV KRFVF+ +KIT+    L+V++ +  K    TR+E++++LI+K+V+ S          
Sbjct: 250 TVSKRFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVMKST--------- 309

Query: 304 PSSLLQV----VNIRKRVVPPLPEALKGNII--------------------TFYVVPLQE 363
           P+  L V    VN+RK++ PPL +   GN+                     T      + 
Sbjct: 310 PAGFLPVVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSES 369

Query: 364 EV---ELWEVVRDMKRNFEEF------CDKYPQNYEAAEWISLHKLFMEESFGRMAQNFY 423
           +V   EL + +  M+   ++        +K  QN+ +    S+ K+   E       NF+
Sbjct: 370 QVVLHELHDFIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDVEVI-----NFW 429

Query: 424 TCSSWCNVGLYEVDFGCGKPAWITMPDIVR--KNSIVLMDLKHELEGMEALVCLDKDVMA 431
             SSWC +GLYE+DFG GKP W+T+   ++  KN   + D K   EG+E      +D MA
Sbjct: 430 -ISSWCRMGLYEIDFGWGKPIWVTVDPNIKPNKNCFFMNDTKCG-EGIEVWASFLEDDMA 464

BLAST of MC08g1719 vs. NCBI nr
Match: XP_022143301.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 844 bits (2181), Expect = 1.34e-308
Identity = 418/441 (94.78%), Postives = 418/441 (94.78%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS
Sbjct: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120
           EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP
Sbjct: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120

Query: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP 180
           EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP
Sbjct: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP 180

Query: 181 QFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTVVKRFVFEGSK 240
           QFNAASFFPPGRKPFPL                       VREQRVDNTVVKRFVFEGSK
Sbjct: 181 QFNAASFFPPGRKPFPL-----------------------VREQRVDNTVVKRFVFEGSK 240

Query: 241 ITALKVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPL 300
           ITALKVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPL
Sbjct: 241 ITALKVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPL 300

Query: 301 PEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEES 360
           PEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEES
Sbjct: 301 PEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEES 360

Query: 361 FGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVC 420
           FGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVC
Sbjct: 361 FGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVC 418

Query: 421 LDKDVMAAFERNEELLCFGCL 441
           LDKDVMAAFERNEELLCFGCL
Sbjct: 421 LDKDVMAAFERNEELLCFGCL 418

BLAST of MC08g1719 vs. NCBI nr
Match: XP_022143303.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 693 bits (1788), Expect = 1.04e-248
Identity = 348/444 (78.38%), Postives = 376/444 (84.68%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           MEFEIV+KESI+PSSPTPPELRHL  S FDA +PC Y++CVFFYHNN + SA  RRRSHS
Sbjct: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNN-ESSAGGRRRSHS 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120
           E LK SLSEVLTHYYPFAGRLIDGASVECN+ GATFTEANL+CPMSDVTK+DEIWKLLCP
Sbjct: 61  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCP 120

Query: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGA---LLP 180
            DGTEINPILSVQLTRFQCGGEAICVFLSHK+SD++TFATFMNDWASIAR+      LLP
Sbjct: 121 GDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLP 180

Query: 181 VSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTVVKRFVFE 240
           VSPQFNA+SFFPPGR+  PL                       VREQR D+ VVKRF FE
Sbjct: 181 VSPQFNASSFFPPGRESCPL-----------------------VREQRRDHIVVKRFAFE 240

Query: 241 GSKITALKVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVV 300
             KI ALK MVSEKVENPTR +LLSALIYKA VIS GG  K+P PPSSLLQ+VNIRKRVV
Sbjct: 241 SPKIAALKAMVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVV 300

Query: 301 PPLPEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFM 360
           PPLPEALKGNIITF+VVPLQEEVELWEVVRDMKRNFEEFCDKYP+NYEA EW SLHKL+M
Sbjct: 301 PPLPEALKGNIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYM 360

Query: 361 EESFGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEA 420
           EESFGRMAQNFY CSSWCNVGLYEVDFG GKPAWITMPDI+R+NSI LMD K   EGMEA
Sbjct: 361 EESFGRMAQNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDG-EGMEA 419

Query: 421 LVCLDKDVMAAFERNEELLCFGCL 441
           +VCLDK+VMA FE+N+ELL FGCL
Sbjct: 421 VVCLDKEVMAVFEKNQELLSFGCL 419

BLAST of MC08g1719 vs. NCBI nr
Match: KAG6581251.1 (Tabersonine-19-hydroxy-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 393 bits (1010), Expect = 3.36e-130
Identity = 234/467 (50.11%), Postives = 294/467 (62.96%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           ME EIV+KE+I+P S TPP+L  L LS+FD   P  Y   ++FY NNN   + S  R   
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRG-- 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----DEI 120
            +LK SLS+ LT YYPFAGRL DG + ++CN++GATF EA L+CPMS+V  +     DEI
Sbjct: 61  RLLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEI 120

Query: 121 WKLLCPED----GTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIAR 180
            KLLC ED        +P+LSVQLT+F+CGGE + V L+HK++D+ TFA FMNDWASIAR
Sbjct: 121 LKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIAR 180

Query: 181 SPGALLP--VSPQFNAASFFPP----GRKPFPLERNLKFRANRILPFNLLFLFLKLVREQ 240
           S G + P  V P F+AAS F P    G  P   E +                      ++
Sbjct: 181 SSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGS--------------------KK 240

Query: 241 RVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSALIYKAVVIS----AGGFEKRP 300
           R +N   +R VFEGSKI ALK MVS+KVENPTR+++L+A IYKA+V +    +GG  + P
Sbjct: 241 REENVRSRRMVFEGSKIRALKAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREP 300

Query: 301 PPPSSLLQVVNIRKRVVPPLPEALKGNIITFY-----VVPLQEEVELWEVVRDMKRNFEE 360
              +S+LQ +N+R RV PPLP  L GNI++F+         Q E+ELW VV DMKRNFEE
Sbjct: 301 ---TSILQFINLRSRVEPPLPGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEE 360

Query: 361 FCDKYPQNYEAAEWISLHKLFMEESFGR-MAQNF---YTCSSWCNVGLYEVDFGCGKPAW 420
           FC K P+NY   E  S  K +++ESF   M  N    Y CSSWC   LYE DFG G P W
Sbjct: 361 FCRKLPRNYRDEEGSSALKSWIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIW 420

Query: 421 ITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 438
           I +P  + KN+I LMD K   EG+EA+VCLDKD MA FE NEELL F
Sbjct: 421 IAVPAFLSKNTIGLMDAKDG-EGIEAIVCLDKDEMAVFETNEELLSF 441

BLAST of MC08g1719 vs. NCBI nr
Match: XP_023528950.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 392 bits (1007), Expect = 9.54e-130
Identity = 235/467 (50.32%), Postives = 294/467 (62.96%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           ME EIV+KE+I+P S TPP+L  L LS+FD   P  Y   ++FY NNN   + S  R   
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRG-- 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----DEI 120
            +LK SLS+ LT YYPFAGRL DG + ++CN++GATF EA L+CPMS+V  +     DEI
Sbjct: 61  RLLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFLEAKLRCPMSEVMNTFDLHHDEI 120

Query: 121 WKLLCPED----GTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIAR 180
            KLLC ED        +P+LSVQLT+F+CGGE + V L+HK++D+ TFA FMNDWASIAR
Sbjct: 121 LKLLCFEDIKGNVQTFDPLLSVQLTQFECGGEIMYVSLAHKVADLATFANFMNDWASIAR 180

Query: 181 SPGALLP--VSPQFNAASFFPP----GRKPFPLERNLKFRANRILPFNLLFLFLKLVREQ 240
           S G   P  V P F+AAS F P    G  P   E +                      ++
Sbjct: 181 SSGGSDPPVVCPLFHAASLFRPELNGGGDPSEGEGSGS--------------------KK 240

Query: 241 RVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSALIYKAVVIS----AGGFEKRP 300
           R +N   +R VFEGSKI ALK MVS+KVENPTR+++L+A IYKA+V +    +GGF + P
Sbjct: 241 REENVRSRRMVFEGSKINALKAMVSKKVENPTRVQVLTAFIYKAMVSTKNALSGGFSREP 300

Query: 301 PPPSSLLQVVNIRKRVVPPLPEALKGNIITFY-----VVPLQEEVELWEVVRDMKRNFEE 360
              +S+LQ +N+R RV PPLP  L GNI++F+         Q E+ELW VV DMKRNFEE
Sbjct: 301 ---TSILQFINLRSRVEPPLPGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEE 360

Query: 361 FCDKYPQNYEAAEWISLHKLFMEESFGR-MAQNF---YTCSSWCNVGLYEVDFGCGKPAW 420
           FC K P+NY   E  S  K +++ESF   M  N    Y CSSWC   LYE DFG G P W
Sbjct: 361 FCRKLPRNYRDEEGSSGLKSWIKESFETWMIDNDGRKYGCSSWCRFPLYEADFGWGTPIW 420

Query: 421 ITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 438
           I +P  + KN+I LMD K   EG+EA+VCLDKD MA FE NEELL F
Sbjct: 421 IAVPAFLSKNTIGLMDAKDG-EGIEAIVCLDKDEMAVFETNEELLSF 441

BLAST of MC08g1719 vs. NCBI nr
Match: XP_022935661.1 (vinorine synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 391 bits (1005), Expect = 1.91e-129
Identity = 233/467 (49.89%), Postives = 293/467 (62.74%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           ME EIV+KE+I+P S TPP+L  L LS+FD   P  Y   ++FY NNN   + S  R   
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRG-- 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----DEI 120
            +LK SLS+ LT YYPFAGRL DG + ++CN++GATF EA L+CPMS+V  +     DEI
Sbjct: 61  RLLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEI 120

Query: 121 WKLLCPED----GTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIAR 180
            KLLC ED        +P+LSVQLT+F+CGGE + V L+HK++D+ TFA FMNDWASIAR
Sbjct: 121 LKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIAR 180

Query: 181 SPGALLP--VSPQFNAASFFPP----GRKPFPLERNLKFRANRILPFNLLFLFLKLVREQ 240
           S G + P  V P F+AAS F P    G  P   E +                      ++
Sbjct: 181 SSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGS--------------------KK 240

Query: 241 RVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSALIYKAVVIS----AGGFEKRP 300
           R +N   +R VFEGSKI ALK MVS+KVENPTR+++L+A IYKA+V +    +GG  + P
Sbjct: 241 REENVRSRRMVFEGSKIRALKAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREP 300

Query: 301 PPPSSLLQVVNIRKRVVPPLPEALKGNIITFY-----VVPLQEEVELWEVVRDMKRNFEE 360
              +S+LQ +N+R RV PPLP  L GNI++F+         Q E+ELW VV DMKRNFEE
Sbjct: 301 ---TSILQFINLRSRVEPPLPGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEE 360

Query: 361 FCDKYPQNYEAAEWISLHKLFMEESFGR-MAQNF---YTCSSWCNVGLYEVDFGCGKPAW 420
           FC K P+NY   E     K +++ESF   M  N    Y CSSWC   LYE DFG G P W
Sbjct: 361 FCRKLPRNYRDEEGSPALKSWIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIW 420

Query: 421 ITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 438
           I +P  + KN+I LMD K   EG+EA+VCLDKD MA FE NEELL F
Sbjct: 421 IAVPAFLSKNTIGLMDAKDG-EGIEAIVCLDKDEMAVFETNEELLSF 441

BLAST of MC08g1719 vs. ExPASy TrEMBL
Match: A0A6J1CPW0 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111013206 PE=3 SV=1)

HSP 1 Score: 844 bits (2181), Expect = 6.50e-309
Identity = 418/441 (94.78%), Postives = 418/441 (94.78%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS
Sbjct: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120
           EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP
Sbjct: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120

Query: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP 180
           EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP
Sbjct: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP 180

Query: 181 QFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTVVKRFVFEGSK 240
           QFNAASFFPPGRKPFPL                       VREQRVDNTVVKRFVFEGSK
Sbjct: 181 QFNAASFFPPGRKPFPL-----------------------VREQRVDNTVVKRFVFEGSK 240

Query: 241 ITALKVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPL 300
           ITALKVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPL
Sbjct: 241 ITALKVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPL 300

Query: 301 PEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEES 360
           PEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEES
Sbjct: 301 PEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEES 360

Query: 361 FGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVC 420
           FGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVC
Sbjct: 361 FGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVC 418

Query: 421 LDKDVMAAFERNEELLCFGCL 441
           LDKDVMAAFERNEELLCFGCL
Sbjct: 421 LDKDVMAAFERNEELLCFGCL 418

BLAST of MC08g1719 vs. ExPASy TrEMBL
Match: A0A6J1CQD5 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111013207 PE=3 SV=1)

HSP 1 Score: 693 bits (1788), Expect = 5.02e-249
Identity = 348/444 (78.38%), Postives = 376/444 (84.68%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           MEFEIV+KESI+PSSPTPPELRHL  S FDA +PC Y++CVFFYHNN + SA  RRRSHS
Sbjct: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYHNN-ESSAGGRRRSHS 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120
           E LK SLSEVLTHYYPFAGRLIDGASVECN+ GATFTEANL+CPMSDVTK+DEIWKLLCP
Sbjct: 61  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCP 120

Query: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGA---LLP 180
            DGTEINPILSVQLTRFQCGGEAICVFLSHK+SD++TFATFMNDWASIAR+      LLP
Sbjct: 121 GDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLP 180

Query: 181 VSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTVVKRFVFE 240
           VSPQFNA+SFFPPGR+  PL                       VREQR D+ VVKRF FE
Sbjct: 181 VSPQFNASSFFPPGRESCPL-----------------------VREQRRDHIVVKRFAFE 240

Query: 241 GSKITALKVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVV 300
             KI ALK MVSEKVENPTR +LLSALIYKA VIS GG  K+P PPSSLLQ+VNIRKRVV
Sbjct: 241 SPKIAALKAMVSEKVENPTRTQLLSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVV 300

Query: 301 PPLPEALKGNIITFYVVPLQEEVELWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFM 360
           PPLPEALKGNIITF+VVPLQEEVELWEVVRDMKRNFEEFCDKYP+NYEA EW SLHKL+M
Sbjct: 301 PPLPEALKGNIITFFVVPLQEEVELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYM 360

Query: 361 EESFGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEA 420
           EESFGRMAQNFY CSSWCNVGLYEVDFG GKPAWITMPDI+R+NSI LMD K   EGMEA
Sbjct: 361 EESFGRMAQNFYLCSSWCNVGLYEVDFGWGKPAWITMPDIIRRNSIGLMDAKDG-EGMEA 419

Query: 421 LVCLDKDVMAAFERNEELLCFGCL 441
           +VCLDK+VMA FE+N+ELL FGCL
Sbjct: 421 VVCLDKEVMAVFEKNQELLSFGCL 419

BLAST of MC08g1719 vs. ExPASy TrEMBL
Match: A0A6J1F5C1 (vinorine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442451 PE=3 SV=1)

HSP 1 Score: 391 bits (1005), Expect = 9.26e-130
Identity = 233/467 (49.89%), Postives = 293/467 (62.74%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           ME EIV+KE+I+P S TPP+L  L LS+FD   P  Y   ++FY NNN   + S  R   
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRG-- 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----DEI 120
            +LK SLS+ LT YYPFAGRL DG + ++CN++GATF EA L+CPMS+V  +     DEI
Sbjct: 61  RLLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEI 120

Query: 121 WKLLCPED----GTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIAR 180
            KLLC ED        +P+LSVQLT+F+CGGE + V L+HK++D+ TFA FMNDWASIAR
Sbjct: 121 LKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIAR 180

Query: 181 SPGALLP--VSPQFNAASFFPP----GRKPFPLERNLKFRANRILPFNLLFLFLKLVREQ 240
           S G + P  V P F+AAS F P    G  P   E +                      ++
Sbjct: 181 SSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGS--------------------KK 240

Query: 241 RVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSALIYKAVVIS----AGGFEKRP 300
           R +N   +R VFEGSKI ALK MVS+KVENPTR+++L+A IYKA+V +    +GG  + P
Sbjct: 241 REENVRSRRMVFEGSKIRALKAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREP 300

Query: 301 PPPSSLLQVVNIRKRVVPPLPEALKGNIITFY-----VVPLQEEVELWEVVRDMKRNFEE 360
              +S+LQ +N+R RV PPLP  L GNI++F+         Q E+ELW VV DMKRNFEE
Sbjct: 301 ---TSILQFINLRSRVEPPLPGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEE 360

Query: 361 FCDKYPQNYEAAEWISLHKLFMEESFGR-MAQNF---YTCSSWCNVGLYEVDFGCGKPAW 420
           FC K P+NY   E     K +++ESF   M  N    Y CSSWC   LYE DFG G P W
Sbjct: 361 FCRKLPRNYRDEEGSPALKSWIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIW 420

Query: 421 ITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 438
           I +P  + KN+I LMD K   EG+EA+VCLDKD MA FE NEELL F
Sbjct: 421 IAVPAFLSKNTIGLMDAKDG-EGIEAIVCLDKDEMAVFETNEELLSF 441

BLAST of MC08g1719 vs. ExPASy TrEMBL
Match: A0A0A0KV47 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001560 PE=3 SV=1)

HSP 1 Score: 377 bits (969), Expect = 2.15e-124
Identity = 211/465 (45.38%), Postives = 286/465 (61.51%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           M+ EIV+KE+I+P   TPPEL+  +L  FD   P  YL  ++FY N+          SHS
Sbjct: 2   MQLEIVSKETIKPLFSTPPELKLHHLCLFDRHAPYLYLHFLYFYQNS----------SHS 61

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVT-----KSDEIW 120
           ++LKHSLS+ LT YYPFAGRL D  S++CN++GAT  EA L+CPMS+       + DE  
Sbjct: 62  KLLKHSLSKALTFYYPFAGRLKDDCSIDCNDMGATILEARLRCPMSEFMNVYNLRHDEAL 121

Query: 121 KLLCPEDGTE------INPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIA 180
           KL+C +D  +       NP+L VQLTRF+CGGE +CVFLSHKL+D  TF  FMN WAS++
Sbjct: 122 KLVCFDDMNDGSKDQRYNPLLCVQLTRFECGGEVLCVFLSHKLADASTFTNFMNHWASLS 181

Query: 181 RSPGALLPV--SPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVD 240
           R+    +P+   P+F+A S F        L    K                     +R  
Sbjct: 182 RTGDHDMPLLPPPRFDAGSLFEAATVDDVLGEGSKI-------------------VKREK 241

Query: 241 NTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSALIYKAVVISAGG----FEKRPPPP 300
           N V KR VF+ SKI ALK  V +KVENP+R+++L+A IYKA + ++         R P  
Sbjct: 242 NLVSKRMVFKASKIAALKATVCDKVENPSRVQILAAFIYKAAISASKSTLSIHHSRTPIT 301

Query: 301 SSLLQV-VNIRKRVVPPLPEALKGNIITFYVVPL-----QEEVELWEVVRDMKRNFEEFC 360
           + LL++ +N+R R++PP+P  L GNII+F++        Q+E+EL  ++ DMKRN +EFC
Sbjct: 302 NYLLRIAINLRTRLIPPMPATLSGNIISFFLTSATTTAEQKEIELSSLLSDMKRNMKEFC 361

Query: 361 DKYPQNYEAAEWISLHKLFMEESFGRMA----QNFYTCSSWCNVGLYEVDFGCGKPAWIT 420
           +KYP+NY+A EW SL+KL ++ES  RM     Q  Y CSSWC    Y+ DFG GKP WIT
Sbjct: 362 NKYPRNYKAEEWSSLYKLHIKESMERMNNPEDQIVYACSSWCRFPFYDADFGWGKPVWIT 421

Query: 421 MPDIVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 438
            P I+ KN I+LMD K   EG+EA V L+++ MA FE+NEELL +
Sbjct: 422 TPTILSKNVIILMDAKDG-EGIEAFVSLEEEEMAVFEQNEELLSY 436

BLAST of MC08g1719 vs. ExPASy TrEMBL
Match: A0A6J1F342 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111441702 PE=3 SV=1)

HSP 1 Score: 371 bits (953), Expect = 1.46e-121
Identity = 222/481 (46.15%), Postives = 296/481 (61.54%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQC--SADSRRRS 60
           ME EI+ KE+I+P + T PELR+L    FD   P  Y+Q ++FY NNN    SA  RRR 
Sbjct: 1   MELEILCKETIKPITSTSPELRNLPRCLFDQQAPNIYMQLLYFYTNNNAVVSSAGCRRR- 60

Query: 61  HSEVLKHSLSEVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----D 120
              +LK SLS+ LT YYPFAGRL DG + ++CN++GA F+EA L+C M +   +     +
Sbjct: 61  ---LLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGAIFSEAKLRCSMLEFMNNFNLERE 120

Query: 121 EIWKLLCPED--GTE--INPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASI 180
           EI KL    D  G +   NP+L +QLT+F+CGG+ + VF SHK++D+ +   FMNDWASI
Sbjct: 121 EILKLTFAVDINGNDPTFNPLLLIQLTQFECGGDVMFVFCSHKIADLASLTNFMNDWASI 180

Query: 181 ARSP--GALLPVSPQFNAASFFPP----GRKPFPLERNLKFRANRILPFNLLFLFLKLVR 240
           ARS   G L  V+P+ N  S+FPP    G  PF         +  +              
Sbjct: 181 ARSSDDGDLPVVNPEINGVSYFPPELDAGGDPFGGGDG----SGTV-------------- 240

Query: 241 EQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSALIYKAVV---ISAGGFEKR 300
            +R +N   KR VFEGSKI ALK MVSE+VENPTR+++L+A IYKAV+    SA G+ K+
Sbjct: 241 -RRDENVCSKRLVFEGSKIAALKAMVSEEVENPTRVQILTAFIYKAVLSAKFSATGYNKK 300

Query: 301 --------------PPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPL----QEEVE 360
                           P +SLLQ++N+R+RV PPLPE L GNIITF++       + E+E
Sbjct: 301 ILTFVCGANLFYKGSRPATSLLQLINLRRRVEPPLPETLTGNIITFFIASWTAAEKREME 360

Query: 361 LWEVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEESFGRMA----QNFYTCSSWCNV 420
           L  +V DMK +FEEFC  +P+NY    W SLHKL  +ES         ++ YTC+SWC  
Sbjct: 361 LCNMVGDMKTSFEEFCKTFPRNYRDEAWSSLHKLHAKESMETWRNPGERSVYTCTSWCRF 420

Query: 421 GLYEVDFGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLC 438
            +YE DFG GKP WIT+P+   KN I+LMD K+  +G+EA+V LDK+ MAAFE+N+ELL 
Sbjct: 421 PMYEADFGWGKPEWITVPEAPWKNMILLMDAKNG-DGIEAMVSLDKEEMAAFEQNQELLS 457

BLAST of MC08g1719 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 226.1 bits (575), Expect = 5.5e-59
Identity = 155/455 (34.07%), Postives = 232/455 (50.99%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           M  ++V+++ I+PSSPTP  L+   LS  +   P  +   VFFY  NN      +     
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSI----KPTEQL 60

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV---TKSDEIWKL 120
           ++LK SLSE LTH+YP AGRL    S++CN+ GA F EA +  P+S++     SD + +L
Sbjct: 61  QMLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQL 120

Query: 121 L-CPEDGTEINP-ILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGAL 180
           +    D  E    +L  Q + F+CG  +I V +SHKL+D  +   FM  WA+I+ S G++
Sbjct: 121 IPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAIS-SRGSI 180

Query: 181 LPV-SPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTVVKRF 240
             + +P F+    FPPG           F      P         +  E  ++ T+ KRF
Sbjct: 181 KTIGAPVFDTVKIFPPG----------NFSETSPAPV--------VEPEIMMNQTLSKRF 240

Query: 241 VFEGSKITALKVMVSE-KVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIR 300
           +F+ S I AL+   S  +V  PTR+E +SALI+K+  + A         PS L   V++R
Sbjct: 241 IFDSSSIQALQAKASSFEVNQPTRVEAVSALIWKS-AMKATRTVSGTSKPSILANSVSLR 300

Query: 301 KRVVPPLPEALKGNIITFYVVPLQEEVE------LWEVVRDMKRNFEEF-CDKYPQNYEA 360
            RV PP  +   GN+++++    +E +       L   +R  K+ F +    K   N  A
Sbjct: 301 SRVSPPFTKNSIGNLVSYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNA 360

Query: 361 AEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIVLM 420
            E I  ++    +       +FY  SS C  GLYE DFG GKP W+  P + +KN + L+
Sbjct: 361 TEIICSYQKEAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLL 420

Query: 421 DLKHELEGMEALVCLDKDVMAAFERNEELLCFGCL 442
           D K E  G+EA V L++  M  FE++ ELL F  L
Sbjct: 421 DTK-EAGGIEAWVNLNEQEMNLFEQDRELLQFASL 430

BLAST of MC08g1719 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 210.3 bits (534), Expect = 3.1e-54
Identity = 146/460 (31.74%), Postives = 238/460 (51.74%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           M+ E + KE I+PSS TP +L+ L LS +D  +P  Y     FY  ++  S +      S
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPE----QSS 63

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANL-QCPMSDVTKSDE---IWK 120
             LK SL+E LT +YP AGR I G +++CN+ GA F +A +   P+SD  +S +   + +
Sbjct: 64  HKLKTSLAETLTKFYPLAGR-IKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQ 123

Query: 121 LL------CPEDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIAR 180
           LL       P +     P+L V+ T F CGG AI + ++HK++D  + +TF+  WA++AR
Sbjct: 124 LLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMAR 183

Query: 181 SPGALLPVSPQFNAASFFPPGRK--PFPLERNLKFRANRILPFNLLFLFLKLVREQRVDN 240
                +   P F AA+F+PP  +   FP++ N    AN+I                   +
Sbjct: 184 GEAGDIVADPVFAAANFYPPANELFKFPVDEN----ANKI-------------------S 243

Query: 241 TVVKRFVFEGSKITALKVMVSEK--VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSL 300
            + KRFVF  SK+  L+   + +  V  P R+E ++AL++KA V +A            L
Sbjct: 244 QITKRFVFSASKLEELRTKAASEDFVGRPKRVESVTALLWKAFVAAASS-NTNACDMKVL 303

Query: 301 LQVVNIRKRVVPPLPEALKGNIITFYVV---PLQEEVELWEVVRDMKRNFEEFCDKYPQN 360
           +Q  N+R ++   L E+L GNI+   VV     QEE+++ + VRD+++  ++  +   + 
Sbjct: 304 IQPSNLRPKIPSLLAESLIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEE 363

Query: 361 YEAAEWISLHKL--FMEESFGRMAQNF---YTCSSWCNVGLYEVDFGCGKPAWITMPDIV 420
             ++  +   KL   M  ++ +++      YT SSWC + LYE  FG G P WI      
Sbjct: 364 GGSSSAMIGSKLANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAP 423

Query: 421 RKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCF 439
              ++ ++    + +G+EA V L ++ M + E+N ELL F
Sbjct: 424 MLENVTMLIDSKDGQGIEAFVTLPEENMLSLEQNTELLAF 434

BLAST of MC08g1719 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 209.1 bits (531), Expect = 7.0e-54
Identity = 155/465 (33.33%), Postives = 236/465 (50.75%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           M+ E ++KE I+PSSPTP  L+ L LS +D  +P  Y     FY  N+  S +      S
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHT----S 65

Query: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANL-QCPMSDVTKS---DEIWK 120
             LK SLSE LT +YP AGR I G +V+C + GA F +A +  CP+++  K    D + +
Sbjct: 66  HKLKTSLSETLTKFYPLAGR-ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQ 125

Query: 121 LL------CPEDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIAR 180
           LL       P       P+L V+ T F CGG AI + ++HK++D  + +TF+  WA+ AR
Sbjct: 126 LLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATAR 185

Query: 181 SPG-ALLPVSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNT 240
               A    SP F  A+F+PP  + F L  +                      +    ++
Sbjct: 186 GENDAAAMESPVFAGANFYPPANEAFKLPAD---------------------EQAGKRSS 245

Query: 241 VVKRFVFEGSKITAL--KVMVSEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLL 300
           + KRFVFE SK+  L  K    E V+ PTR+E ++ALI+K  V S+   +        L+
Sbjct: 246 ITKRFVFEASKVEDLRTKAASEETVDQPTRVESVTALIWKCFVASS---KTTTCDHKVLV 305

Query: 301 QVVNIRKRVVPPLPEALKGNIITFYVV---PLQEEVELWEVVRDMKRNFEE----FCDKY 360
           Q+ N+R ++   L E+  GN++   VV       EV++ E VRD+++  EE      D+ 
Sbjct: 306 QLANLRSKIPSLLQESSIGNLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEG 365

Query: 361 PQNYEAAEWISLHKLFMEESFGRMAQNF---YTCSSWCNVGLYEVDFGCGKPAWIT-MPD 420
             +  ++   S     M  ++ R++      YT SSWC + LYE  FG   P W+     
Sbjct: 366 GSSDSSSMIGSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVS 425

Query: 421 IVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCFGCL 442
            V  N  +L+D K + +G+EA V L ++ M++FE+N ELL F  +
Sbjct: 426 PVLGNLAMLIDSK-DGQGIEAFVTLPEENMSSFEQNPELLAFATM 440

BLAST of MC08g1719 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 192.2 bits (487), Expect = 8.9e-49
Identity = 146/452 (32.30%), Postives = 228/452 (50.44%), Query Frame = 0

Query: 1   MEFEIVNKESIRPSSPT---PPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRR 60
           +E  + ++E ++PS      PP   H +LSF D   P  ++  +FFYHN    S D  R 
Sbjct: 3   LEITVTSQELVKPSPRNLNHPP--CHHHLSFLDQLAPPIFMPFLFFYHNKTNLS-DKERS 62

Query: 61  SHSEVLKHSLSEVLTHYYPFAGRLID-GASVECNNVGATFTEANLQCPMSDVTKSDEIWK 120
            H   +K SLSE+L  YYP AGR+ + G  V CN+VG +F EA   C MS + ++    +
Sbjct: 63  DH---IKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNE 122

Query: 121 L--LCPEDGTEINPI-LSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPG 180
           L  L P +  E++ + L+VQLT F+CGG A+ + LSHKL D ++   F+N WA+ AR   
Sbjct: 123 LNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQT 182

Query: 181 ALLPVSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVDNTVVKR 240
             + ++P F+ A  FP    P  +E                   L +      +N V +R
Sbjct: 183 DEI-ITPSFDLAKMFP----PCDIEN------------------LNMATGITKENIVTRR 242

Query: 241 FVFEGSKITALKVMVS-EKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNI 300
           FVF  S + +L+   S  K    TR+E+LS  I+   + S    + +     +L+  VN+
Sbjct: 243 FVFLRSSVESLRERFSGNKKIRATRVEVLSVFIWSRFMAST-NHDDKTGKIYTLIHPVNL 302

Query: 301 RKRVVPPLPEALKGNIITFYV-VPL-------QEEVELWEVVRDMKRNFEEFCDKYPQNY 360
           R++  P +P+ + GNI+ F V VP+       +E+  L + +R+  R  +    K  Q  
Sbjct: 303 RRQADPDIPDNMFGNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQED 362

Query: 361 EAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVRKNSIV 420
                  L+K       G +    ++ +S C   +YE DFG GKP W+    +  KN + 
Sbjct: 363 NRGHLEFLNKQASGFVNGEIVS--FSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVA 421

Query: 421 LMDLKHELEGMEALVCLDKDVMAAFERNEELL 437
            +D K E +G+EA + LD++ M+ FE +EELL
Sbjct: 423 FIDTK-EGDGIEAWINLDQNDMSRFEADEELL 421

BLAST of MC08g1719 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 191.0 bits (484), Expect = 2.0e-48
Identity = 146/446 (32.74%), Postives = 228/446 (51.12%), Query Frame = 0

Query: 4   EIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHSEVL 63
           EI+++E ++PSSPTP + R L LS  D      Y   + FY  + Q          S  L
Sbjct: 6   EILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLKL 65

Query: 64  KHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKS--DEIWKLLCPE 123
           K SLS+ L  +YP AGR+I G+ VECN+ GA F EA +   +S+  K    E  +LL P 
Sbjct: 66  KKSLSKTLPIFYPLAGRII-GSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLELLIPV 125

Query: 124 DGTEIN----PILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLP 183
           +         P+L +Q   F CGG  I + +SHK++D  + A F+  WA  +R  G  + 
Sbjct: 126 EAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRGLG--IT 185

Query: 184 VSPQFNAASFFPPGRKPFPLERNLKFRANRILPFNLLFLFLKLVREQRVD--NTVVKRFV 243
           + P F A+  FP      PL+          LP         + R++ V+  + V KRFV
Sbjct: 186 LIPSFTASEVFPK-----PLDE---------LPSK------PMDRKEEVEEMSCVTKRFV 245

Query: 244 FEGSKITALKVMVSEK-VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRK 303
           F+ SKI  L+   S   V+NPTR+E ++AL ++ V           P  S L  +VN+R 
Sbjct: 246 FDASKIKKLRAKASRNLVKNPTRVEAVTALFWRCVT-KVSRLSSLTPRTSVLQILVNLRG 305

Query: 304 RVVPPLPEALKGNIITFYVVPLQEEV--ELWEVVRDMKRNFEEFCDKYPQNYEAAEWISL 363
           + V  L E   GN+++  ++  +E     + +VV +++R  E F     +  +++  I  
Sbjct: 306 K-VDSLCENTIGNMLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFE 365

Query: 364 HKLFMEESFGR-MAQNFYTCSSWCNVGLYEVDFGCGKPAWITMPDIVR-KNSIVLMDLKH 423
               + + +GR    + +  +SWC +GLY+ DFG GKP W+T       KN ++L+D K 
Sbjct: 366 LLEEIGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTK- 425

Query: 424 ELEGMEALVCLDKDVMAAFERNEELL 437
           + EG+EA + L ++ M+ FE ++ELL
Sbjct: 426 DGEGIEAWITLTEEQMSLFECDQELL 425