MC08g1683 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1683
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionEpoxide hydrolase 2
LocationMC08: 24807550 .. 24809257 (+)
RNA-Seq ExpressionMC08g1683
SyntenyMC08g1683
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAACCAGAACAGAATGGAGACCATCGAGCACACCACCGTCAAAACCAACGGCGTGAAGATCCACGTGGCCGCCGTGGGAAGCGGGCCGCCGGTGCTCCTCCTCCACGGGTTCCCGGAGCTGTGGTACTCGTGGCGGCACCAGATGGTGTACCTGGCCTCCGCCGGGTACCGCGCGATCGCCCCGGACCTCCGCGGGTACGGGGACTCGGACGCGCCGCCGTCGGCCGGCGCGTACACGGCGCTGCACATAGTGGGCGATTTGATCGGGGTTCTGGACGACCTGGGCATCGAGAAGGTGCTGTTGGTGGGCCACGATTGGGGGGCTTTAATTGCATGGTATTTTTGTCTCTTCAGACCCGATCGGATCAAGGCGCTGGTGAATTTGAGCGTCCAGTTCTTCCCCAGAGATCCCACCACGCCGTTTATTCAAGGCTTCAAAGCTGCTCTTGGGGATCAGTTATATATGGTCAGATTTCAGGTAAATCAACGTTAACCCTCCGTTTGGAATCTCGGATTGGTAGGGAATTTCAAAATCAGAGTTAATTTGAAATCAATTCCATCGTAAAATTCATGGTGGTGGGCGTGTTGTTTCATAGGAACCTGGAAAGGCAGAGGCAGAGTTTGCGACGGTGGACATAAGAGAGTTTTTTAAGAACGTCCTCTCCAATAGGGACCCACGTGCTCCATATTTGCCCAATGAGGTCTTGTTCGAAGGGGTCCCGCCGCCGTCGCTTGCCCCGTGGCTAACGCCGGAGGATATCGACGTTTACGCCGCCAAGTTCGCCCAGACTGGCTTCACCGGCGGCCTCAATTACTACCGAGCTTTTGACCGGTCGGTAACTCTAACTTCTAACGACAACCCTCTCAGTTTACTTACAGTTACTCCAAATGGTTTAAAAATTTTAATGCAGTTGGTTAATGGTTGAAAGTGGATATGGTAATTTGGTGCAGAACTTGGGAGCTGACGGCGCCGTGGACGGCGGCGAAGATTCGCGTTCCGGTGAAGTTCATAGTGGGGGATTTGGATCTGACCTACCATTTTCCAGGAGCTCGGGAGTACATCAATGGCGCCGCCTTCAAAAACGACGTCCCTGGTTTGGAAGAAGTGGTTGTAATTGAAGACGCTTCCCACTTCATTAACCAAGAAAAGCCCCATGAAATCAACTCCCACATCCATAATTTCTTCAAGAAATTTTGTTAAATCATATCATGCGTCTCTGTTTTTGTGTGTTTTCATTGGTGATGGATTCTCTCCAACTTTTCTACAATAAGGATCGAGAAAGGGAATAAAGTACTTCGGCCCAAATTTGCTTGATATATATTAATTAAAATATCGCGGGTTCGAATACCACTTTCGTATTTTTGTTGTATTAAAAAACTCTTGTATTTGGTTGTATTAATTAAAATATATATTAATGAATACTTGTTTGTCAAGACATTAGAAATTGAGACATTTATCATTATTTTCATGGCAAATGAAATTTGCAGTTTCTTTTGAGGTTGGACATATATAGATTATAAAATAAATATGTATTTTGAAATAGAGTGAGAAATTTAAAATTATAAAATAAATATGTATTTTGAAATAGAGTGAGAAATTTAAAATTATAAAATAAATATGTATTTTGAAATAGAGTGAGAAATTTAAAATTATAAAATAAATATGTATTTTGAAATAGAGTGAGAAATTTAAAATTTAATTTTTC

mRNA sequence

CACAACCAGAACAGAATGGAGACCATCGAGCACACCACCGTCAAAACCAACGGCGTGAAGATCCACGTGGCCGCCGTGGGAAGCGGGCCGCCGGTGCTCCTCCTCCACGGGTTCCCGGAGCTGTGGTACTCGTGGCGGCACCAGATGGTGTACCTGGCCTCCGCCGGGTACCGCGCGATCGCCCCGGACCTCCGCGGGTACGGGGACTCGGACGCGCCGCCGTCGGCCGGCGCGTACACGGCGCTGCACATAGTGGGCGATTTGATCGGGGTTCTGGACGACCTGGGCATCGAGAAGGTGCTGTTGGTGGGCCACGATTGGGGGGCTTTAATTGCATGGTATTTTTGTCTCTTCAGACCCGATCGGATCAAGGCGCTGGTGAATTTGAGCGTCCAGTTCTTCCCCAGAGATCCCACCACGCCGTTTATTCAAGGCTTCAAAGCTGCTCTTGGGGATCAGTTATATATGGTCAGATTTCAGGAACCTGGAAAGGCAGAGGCAGAGTTTGCGACGGTGGACATAAGAGAGTTTTTTAAGAACGTCCTCTCCAATAGGGACCCACGTGCTCCATATTTGCCCAATGAGGTCTTGTTCGAAGGGGTCCCGCCGCCGTCGCTTGCCCCGTGGCTAACGCCGGAGGATATCGACGTTTACGCCGCCAAGTTCGCCCAGACTGGCTTCACCGGCGGCCTCAATTACTACCGAGCTTTTGACCGAACTTGGGAGCTGACGGCGCCGTGGACGGCGGCGAAGATTCGCGTTCCGGTGAAGTTCATAGTGGGGGATTTGGATCTGACCTACCATTTTCCAGGAGCTCGGGAGTACATCAATGGCGCCGCCTTCAAAAACGACGTCCCTGGTTTGGAAGAAGTGGTTGTAATTGAAGACGCTTCCCACTTCATTAACCAAGAAAAGCCCCATGAAATCAACTCCCACATCCATAATTTCTTCAAGAAATTTTGTTAAATCATATCATGCGTCTCTGTTTTTGTGTGTTTTCATTGGTGATGGATTCTCTCCAACTTTTCTACAATAAGGATCGAGAAAGGGAATAAAGTACTTCGGCCCAAATTTGCTTGATATATATTAATTAAAATATCGCGGGTTCGAATACCACTTTCGTATTTTTGTTGTATTAAAAAACTCTTGTATTTGGTTGTATTAATTAAAATATATATTAATGAATACTTGTTTGTCAAGACATTAGAAATTGAGACATTTATCATTATTTTCATGGCAAATGAAATTTGCAGTTTCTTTTGAGGTTGGACATATATAGATTATAAAATAAATATGTATTTTGAAATAGAGTGAGAAATTTAAAATTATAAAATAAATATGTATTTTGAAATAGAGTGAGAAATTTAAAATTATAAAATAAATATGTATTTTGAAATAGAGTGAGAAATTTAAAATTATAAAATAAATATGTATTTTGAAATAGAGTGAGAAATTTAAAATTTAATTTTTC

Coding sequence (CDS)

CACAACCAGAACAGAATGGAGACCATCGAGCACACCACCGTCAAAACCAACGGCGTGAAGATCCACGTGGCCGCCGTGGGAAGCGGGCCGCCGGTGCTCCTCCTCCACGGGTTCCCGGAGCTGTGGTACTCGTGGCGGCACCAGATGGTGTACCTGGCCTCCGCCGGGTACCGCGCGATCGCCCCGGACCTCCGCGGGTACGGGGACTCGGACGCGCCGCCGTCGGCCGGCGCGTACACGGCGCTGCACATAGTGGGCGATTTGATCGGGGTTCTGGACGACCTGGGCATCGAGAAGGTGCTGTTGGTGGGCCACGATTGGGGGGCTTTAATTGCATGGTATTTTTGTCTCTTCAGACCCGATCGGATCAAGGCGCTGGTGAATTTGAGCGTCCAGTTCTTCCCCAGAGATCCCACCACGCCGTTTATTCAAGGCTTCAAAGCTGCTCTTGGGGATCAGTTATATATGGTCAGATTTCAGGAACCTGGAAAGGCAGAGGCAGAGTTTGCGACGGTGGACATAAGAGAGTTTTTTAAGAACGTCCTCTCCAATAGGGACCCACGTGCTCCATATTTGCCCAATGAGGTCTTGTTCGAAGGGGTCCCGCCGCCGTCGCTTGCCCCGTGGCTAACGCCGGAGGATATCGACGTTTACGCCGCCAAGTTCGCCCAGACTGGCTTCACCGGCGGCCTCAATTACTACCGAGCTTTTGACCGAACTTGGGAGCTGACGGCGCCGTGGACGGCGGCGAAGATTCGCGTTCCGGTGAAGTTCATAGTGGGGGATTTGGATCTGACCTACCATTTTCCAGGAGCTCGGGAGTACATCAATGGCGCCGCCTTCAAAAACGACGTCCCTGGTTTGGAAGAAGTGGTTGTAATTGAAGACGCTTCCCACTTCATTAACCAAGAAAAGCCCCATGAAATCAACTCCCACATCCATAATTTCTTCAAGAAATTTTGTTAA

Protein sequence

HNQNRMETIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLRGYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNRDPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEKPHEINSHIHNFFKKFC
Homology
BLAST of MC08g1683 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 4.8e-57
Identity = 129/317 (40.69%), Postives = 172/317 (54.26%), Query Frame = 0

Query: 14  VKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLRGYGDSD 73
           V TNGV++ V   G    P V+L HGFPEL YSWRHQ+  LA AGY  +APD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 74  APPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKALVNLSV 133
            P +  AY    +  DL+G+LDD+G E+ + VGHDWGA++ W   L   DR+ A+  LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 134 QFFPRDPTTPFIQGFKAALGDQ-LYMVRFQEPGKAEAEFATVDIREFFKNVLSNRDPRAP 193
              PR    P  Q F++  G+   Y++ FQEPG A+AE      R   + +   R P   
Sbjct: 129 PALPRAQVPP-TQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 194 YLPNEVLFEG-------VPPPS-LAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWE 253
                +L  G       +P P+ L  W++ E++D Y  +F +TGFTGGLN+YR FDR WE
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNWE 248

Query: 254 LTAPWTAAKIRVPVKFIVGDLDLTYHF---PGAREYINGAAFKNDVPGLEEVVVIEDASH 313
            TA      I VP  FI G  D    F     A E I+G       P  E  V+I+ A H
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG-------PYRE--VLIDGAGH 308

Query: 314 FINQEKPHEINSHIHNF 317
           ++ QE+P E+ + +  F
Sbjct: 309 WLQQERPGEVTAALLEF 315

BLAST of MC08g1683 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.1e-51
Identity = 112/301 (37.21%), Postives = 167/301 (55.48%), Query Frame = 0

Query: 18  GVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLRGYGDSDAPPSAG 77
           GV++H   +GSGP V L HGFPE W+SWR+Q+  LA AG+R +A D++GYG+S APP   
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 78  AYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKALVNLSVQFFPRD 137
            Y+   +  D++  L+ LG+ + + +GHDWG ++ W   LF P+R++A+ +L+  F P +
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 138 PTTPFIQGFKA--ALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNRDPRAPYLPNE 197
           P    ++  KA      QLY   FQEPG AEAE    ++   FKN     D       N 
Sbjct: 367 PNVSPMEIIKANPVFDYQLY---FQEPGVAEAELEQ-NLDRTFKNFFRAHD-ETFLTTNR 426

Query: 198 V-----LFEGVP-PPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTAPWTA 257
           V     LF G P  PSL+  +T EDI  Y  +F ++GF G LN+YR  +R W+     + 
Sbjct: 427 VRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSG 486

Query: 258 AKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEKPHEI 311
            KI +P   +  + DL  H P   +++     +N +P L+    I+D  H+   +KP E+
Sbjct: 487 RKILIPALMVTAENDLVLH-PKMSKHM-----ENWIPHLKR-GHIKDCGHWTQIDKPAEL 535

BLAST of MC08g1683 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 4.4e-50
Identity = 110/307 (35.83%), Postives = 166/307 (54.07%), Query Frame = 0

Query: 11  HTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLRGYGDS 70
           + TVK   V++H   +GSGP V L HGFPE WYSWR+Q+  LA AGYR +A D++GYG+S
Sbjct: 241 YVTVKPR-VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGES 300

Query: 71  DAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKALVNLS 130
            APP    Y    +  +++  LD LG+ + + +GHDWG ++ WY  LF P+R++A+ +L+
Sbjct: 301 SAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLN 360

Query: 131 VQFFPRDPTTPFIQGFKA--ALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNRDPR 190
             F P +P    ++  KA      QLY   FQEPG AEAE    ++   FK++    D  
Sbjct: 361 TPFIPANPNMSPLESIKANPVFDYQLY---FQEPGVAEAELEQ-NLSRTFKSLFRASDES 420

Query: 191 APYLPNEV----LFEGVP-PPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWEL 250
              +        LF   P  PSL+  +T E+I  Y  +F ++GF G LN+YR  +R W+ 
Sbjct: 421 VLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKW 480

Query: 251 TAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQ 310
                  KI +P   +  + D     P   +++     ++ +P L+    IED  H+   
Sbjct: 481 ACKSLGRKILIPALMVTAEKDFVL-VPQMSQHM-----EDWIPHLKR-GHIEDCGHWTQM 535

BLAST of MC08g1683 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 1.0e-46
Identity = 106/308 (34.42%), Postives = 164/308 (53.25%), Query Frame = 0

Query: 11  HTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLRGYGDS 70
           + TVK  G+++H   +GSGP + L HGFPE W+SWR+Q+  LA AG+R +A D++GYGDS
Sbjct: 239 YVTVKP-GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDS 298

Query: 71  DAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKALVNLS 130
            +PP    Y    +  +++  LD LGI + + +GHDW  ++ W   LF P+R++A+ +L+
Sbjct: 299 SSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLN 358

Query: 131 VQFFPRDPTTPFIQGFKA--ALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNRDPR 190
             F P DP    ++  ++      QLY   FQEPG AEAE    ++   FK+     D  
Sbjct: 359 TPFMPPDPDVSPMKVIRSIPVFNYQLY---FQEPGVAEAELEK-NMSRTFKSFFRASDET 418

Query: 191 ---APYLPNE---VLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWE 250
              A +   E   +L      P+L+   T E+I+ Y  +F +TGF G LN+YR  +R W+
Sbjct: 419 GFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWK 478

Query: 251 LTAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFIN 310
            +      KI VP   +  + D+      ++        +  +P L+    IED  H+  
Sbjct: 479 WSCKGLGRKILVPALMVTAEKDIVLRPEMSKN------MEKWIPFLKR-GHIEDCGHWTQ 534

BLAST of MC08g1683 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.0e-46
Identity = 103/316 (32.59%), Postives = 166/316 (52.53%), Query Frame = 0

Query: 11  HTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLRGYGDS 70
           + TVK  G+++H   +GSGP + L HGFPE W+SWR+Q+  LA AG+R +A D++GYGDS
Sbjct: 239 YVTVKP-GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDS 298

Query: 71  DAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKALVNLS 130
            +PP    Y    +  +++  L+ LGI + + +GHDW  ++ W   LF P+R++A+ +L+
Sbjct: 299 SSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLN 358

Query: 131 VQFFPRDPTTPFIQGFKA--ALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNRDPR 190
               P +P    ++  ++      QLY   FQEPG AEAE    ++   FK+     D  
Sbjct: 359 TPLMPPNPEVSPMEVIRSIPVFNYQLY---FQEPGVAEAELEK-NMSRTFKSFFRTSDDM 418

Query: 191 APYLPNEV-----LFEGVP-PPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWE 250
                N+      +  G P  P ++   T E+I+ Y  +F ++GF G LN+YR  +R W+
Sbjct: 419 GLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWK 478

Query: 251 LTAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFIN 310
            +      KI VP   +  + D+      ++        +N +P L+    IED  H+  
Sbjct: 479 WSCKALGRKILVPALMVTAEKDIVLRPEMSKN------MENWIPFLKR-GHIEDCGHWTQ 538

Query: 311 QEKPHEINSHIHNFFK 319
            EKP E+N  +  + K
Sbjct: 539 IEKPAEVNQILIKWLK 542

BLAST of MC08g1683 vs. NCBI nr
Match: XP_022155322.1 (uncharacterized protein LOC111022455 [Momordica charantia])

HSP 1 Score: 659 bits (1700), Expect = 4.35e-239
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 65
           METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR
Sbjct: 1   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 60

Query: 66  GYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 125
           GYGDS+APPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA
Sbjct: 61  GYGDSEAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 120

Query: 126 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 185
           LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR
Sbjct: 121 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 180

Query: 186 DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA 245
           DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA
Sbjct: 181 DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA 240

Query: 246 PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK 305
           PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK
Sbjct: 241 PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK 300

Query: 306 PHEINSHIHNFFKKFC 321
           PHEINSHIHNFFKKFC
Sbjct: 301 PHEINSHIHNFFKKFC 316

BLAST of MC08g1683 vs. NCBI nr
Match: XP_038905173.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 568 bits (1463), Expect = 6.25e-203
Identity = 263/318 (82.70%), Postives = 290/318 (91.19%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPD 65
           M+TI HTTV+TNG+K+HVAA G  +GPPVLLLHGFPELWYSWRHQ+V+L+SAGYR IAPD
Sbjct: 1   MDTIHHTTVQTNGIKLHVAAAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSAGYRVIAPD 60

Query: 66  LRGYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRI 125
           L GYGDSDAP S+ +YTALHIVGDL+G+LD+LGIEKVLLVGHDWGALIAWYFCLFRPD+I
Sbjct: 61  LHGYGDSDAPSSSDSYTALHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120

Query: 126 KALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLS 185
           KA V LSVQFFPR+P TPFI+GFKA LGDQ YMVRFQEPGKAE EFATVDIREFFKN LS
Sbjct: 121 KASVILSVQFFPRNPKTPFIEGFKAVLGDQFYMVRFQEPGKAEEEFATVDIREFFKNTLS 180

Query: 186 NRDPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWEL 245
           NRDPRAPYLP EV FEGVPPP LAPWLTPEDID YA KF+ TGFTG LNYYRAFDRTWEL
Sbjct: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPEDIDFYAQKFSHTGFTGSLNYYRAFDRTWEL 240

Query: 246 TAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQ 305
           TAPWT A+I VPVKFIVGDLDLTYHFPGA++YI+G AFK DVPGL+EV+V++DASHFINQ
Sbjct: 241 TAPWTGAQIEVPVKFIVGDLDLTYHFPGAQKYIHGDAFKKDVPGLDEVIVMKDASHFINQ 300

Query: 306 EKPHEINSHIHNFFKKFC 321
           E+PHEIN HIH+FF KFC
Sbjct: 301 ERPHEINCHIHDFFNKFC 318

BLAST of MC08g1683 vs. NCBI nr
Match: XP_022948972.1 (uncharacterized protein LOC111452460 [Cucurbita moschata])

HSP 1 Score: 558 bits (1438), Expect = 3.74e-199
Identity = 253/316 (80.06%), Postives = 289/316 (91.46%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 65
           ME I+HTTVKTNG+ +H+AAVG+GPPVLLLHGFPE WYSWRHQ++YL+S GYRAIAPDLR
Sbjct: 1   MEAIQHTTVKTNGINLHIAAVGTGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPDLR 60

Query: 66  GYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 125
           GYGDSDAPPSA AYT LHIVGDL+G+LD+LGIEKVLLVGHDWGA+IAWY CLFRPDRIKA
Sbjct: 61  GYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGHDWGAIIAWYLCLFRPDRIKA 120

Query: 126 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 185
            V LSVQFFPR+PTTPF+Q FK+ LGDQ YMVRFQ+PG+AE EFATVDI++FF +V+SNR
Sbjct: 121 AVILSVQFFPRNPTTPFVQAFKSVLGDQFYMVRFQQPGQAEEEFATVDIKKFFNDVMSNR 180

Query: 186 DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA 245
           DPRAPYLP EV FEG+PPP  A WLTPEDIDVYA KF++TGFTGGLNYYRAF+RTWELTA
Sbjct: 181 DPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADKFSRTGFTGGLNYYRAFNRTWELTA 240

Query: 246 PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK 305
            WT ++IRVPVK+IVGDLDLTYHFPGA+EYI+G  FK DVPGLEEVVV++D SHFINQE+
Sbjct: 241 AWTKSEIRVPVKYIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVVVMKDTSHFINQER 300

Query: 306 PHEINSHIHNFFKKFC 321
           P+E N+HIH+FFKKFC
Sbjct: 301 PNETNAHIHDFFKKFC 316

BLAST of MC08g1683 vs. NCBI nr
Match: XP_023525204.1 (uncharacterized protein LOC111788875 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 552 bits (1423), Expect = 7.21e-197
Identity = 250/316 (79.11%), Postives = 285/316 (90.19%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 65
           ME I+HT VKTNG+ +H+AAVG+GPPVLLLHGFPE WYSWRHQ++YL+S GYRAIAPDLR
Sbjct: 1   MEAIQHTAVKTNGINLHIAAVGTGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPDLR 60

Query: 66  GYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 125
           GYGDSDAPPSA AYT LHIVGDL+G+LD+LGIEKVLLVGHDWGA+IAWY CLFRPDRIKA
Sbjct: 61  GYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGHDWGAIIAWYLCLFRPDRIKA 120

Query: 126 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 185
            V LSVQ FPR+PTTPF++ FK+ LGDQ YMVRFQ+PG+AE EF TVDI++FFK+V+SNR
Sbjct: 121 AVILSVQVFPRNPTTPFVEAFKSVLGDQFYMVRFQQPGQAEEEFGTVDIKKFFKDVMSNR 180

Query: 186 DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA 245
           DPRAPYLP EV FEG+PPP    WLTPEDIDVYA  F++TGFTGGLNYYRAFDRTWELTA
Sbjct: 181 DPRAPYLPGEVKFEGLPPPPPTTWLTPEDIDVYADMFSRTGFTGGLNYYRAFDRTWELTA 240

Query: 246 PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK 305
            WT A+I+VPVKFIVGDLDLTYHFPGA++YI+G  FK DVPGLEEVVV+ED SHFINQE+
Sbjct: 241 AWTRAEIKVPVKFIVGDLDLTYHFPGAQQYIHGDGFKRDVPGLEEVVVMEDTSHFINQER 300

Query: 306 PHEINSHIHNFFKKFC 321
           P+EIN+HIH+FF KFC
Sbjct: 301 PNEINAHIHDFFNKFC 316

BLAST of MC08g1683 vs. NCBI nr
Match: XP_004152243.1 (uncharacterized protein LOC101204575 [Cucumis sativus] >KGN52824.1 hypothetical protein Csa_014427 [Cucumis sativus])

HSP 1 Score: 551 bits (1421), Expect = 1.57e-196
Identity = 252/318 (79.25%), Postives = 285/318 (89.62%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPD 65
           METI H TV+TNG+ +HVA  G  +GPPVLLLHGFPELWYSWRHQ+++L+S GYR IAPD
Sbjct: 1   METINHITVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSVGYRVIAPD 60

Query: 66  LRGYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRI 125
           LRGYGDSDAPPS+  YTALHIVGD++G+L++LGI+KVLLVGHDWGALIAWYFCLFRPDRI
Sbjct: 61  LRGYGDSDAPPSSDTYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 126 KALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLS 185
           KA V LSVQFFPR+P   F++GFKA LGDQ YMVRFQEPGKAE EFA+VDIREFFKNV+S
Sbjct: 121 KASVILSVQFFPRNPKVSFVEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 186 NRDPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWEL 245
           NRDP APYLP E  FEGVPPPSLAPWLTP+DID YA KF+ +GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEEKFEGVPPPSLAPWLTPQDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 246 TAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQ 305
           TAPWTAA+I+VPVKFIVGDLDLTYHFPG ++YING AF+ DVPGLEEV+V++D SHFINQ
Sbjct: 241 TAPWTAAEIKVPVKFIVGDLDLTYHFPGGQDYINGDAFRKDVPGLEEVIVMKDTSHFINQ 300

Query: 306 EKPHEINSHIHNFFKKFC 321
           E+P EIN HIH+FF KFC
Sbjct: 301 ERPDEINCHIHDFFNKFC 318

BLAST of MC08g1683 vs. ExPASy TrEMBL
Match: A0A6J1DP12 (uncharacterized protein LOC111022455 OS=Momordica charantia OX=3673 GN=LOC111022455 PE=4 SV=1)

HSP 1 Score: 659 bits (1700), Expect = 2.11e-239
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 65
           METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR
Sbjct: 1   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 60

Query: 66  GYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 125
           GYGDS+APPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA
Sbjct: 61  GYGDSEAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 120

Query: 126 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 185
           LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR
Sbjct: 121 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 180

Query: 186 DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA 245
           DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA
Sbjct: 181 DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA 240

Query: 246 PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK 305
           PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK
Sbjct: 241 PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK 300

Query: 306 PHEINSHIHNFFKKFC 321
           PHEINSHIHNFFKKFC
Sbjct: 301 PHEINSHIHNFFKKFC 316

BLAST of MC08g1683 vs. ExPASy TrEMBL
Match: A0A6J1GAQ3 (uncharacterized protein LOC111452460 OS=Cucurbita moschata OX=3662 GN=LOC111452460 PE=4 SV=1)

HSP 1 Score: 558 bits (1438), Expect = 1.81e-199
Identity = 253/316 (80.06%), Postives = 289/316 (91.46%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 65
           ME I+HTTVKTNG+ +H+AAVG+GPPVLLLHGFPE WYSWRHQ++YL+S GYRAIAPDLR
Sbjct: 1   MEAIQHTTVKTNGINLHIAAVGTGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPDLR 60

Query: 66  GYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 125
           GYGDSDAPPSA AYT LHIVGDL+G+LD+LGIEKVLLVGHDWGA+IAWY CLFRPDRIKA
Sbjct: 61  GYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGHDWGAIIAWYLCLFRPDRIKA 120

Query: 126 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 185
            V LSVQFFPR+PTTPF+Q FK+ LGDQ YMVRFQ+PG+AE EFATVDI++FF +V+SNR
Sbjct: 121 AVILSVQFFPRNPTTPFVQAFKSVLGDQFYMVRFQQPGQAEEEFATVDIKKFFNDVMSNR 180

Query: 186 DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA 245
           DPRAPYLP EV FEG+PPP  A WLTPEDIDVYA KF++TGFTGGLNYYRAF+RTWELTA
Sbjct: 181 DPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADKFSRTGFTGGLNYYRAFNRTWELTA 240

Query: 246 PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK 305
            WT ++IRVPVK+IVGDLDLTYHFPGA+EYI+G  FK DVPGLEEVVV++D SHFINQE+
Sbjct: 241 AWTKSEIRVPVKYIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVVVMKDTSHFINQER 300

Query: 306 PHEINSHIHNFFKKFC 321
           P+E N+HIH+FFKKFC
Sbjct: 301 PNETNAHIHDFFKKFC 316

BLAST of MC08g1683 vs. ExPASy TrEMBL
Match: A0A0A0KV84 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001910 PE=4 SV=1)

HSP 1 Score: 551 bits (1421), Expect = 7.58e-197
Identity = 252/318 (79.25%), Postives = 285/318 (89.62%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPD 65
           METI H TV+TNG+ +HVA  G  +GPPVLLLHGFPELWYSWRHQ+++L+S GYR IAPD
Sbjct: 1   METINHITVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSVGYRVIAPD 60

Query: 66  LRGYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRI 125
           LRGYGDSDAPPS+  YTALHIVGD++G+L++LGI+KVLLVGHDWGALIAWYFCLFRPDRI
Sbjct: 61  LRGYGDSDAPPSSDTYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 126 KALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLS 185
           KA V LSVQFFPR+P   F++GFKA LGDQ YMVRFQEPGKAE EFA+VDIREFFKNV+S
Sbjct: 121 KASVILSVQFFPRNPKVSFVEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 186 NRDPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWEL 245
           NRDP APYLP E  FEGVPPPSLAPWLTP+DID YA KF+ +GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEEKFEGVPPPSLAPWLTPQDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 246 TAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQ 305
           TAPWTAA+I+VPVKFIVGDLDLTYHFPG ++YING AF+ DVPGLEEV+V++D SHFINQ
Sbjct: 241 TAPWTAAEIKVPVKFIVGDLDLTYHFPGGQDYINGDAFRKDVPGLEEVIVMKDTSHFINQ 300

Query: 306 EKPHEINSHIHNFFKKFC 321
           E+P EIN HIH+FF KFC
Sbjct: 301 ERPDEINCHIHDFFNKFC 318

BLAST of MC08g1683 vs. ExPASy TrEMBL
Match: A0A1S3BXU7 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494759 PE=4 SV=1)

HSP 1 Score: 551 bits (1419), Expect = 1.53e-196
Identity = 256/318 (80.50%), Postives = 287/318 (90.25%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPD 65
           METI+HT V+TNGV +HVA  G  +GPPVLLLHGFPELWYSWR Q+++L+SAGYR IAPD
Sbjct: 1   METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 60

Query: 66  LRGYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRI 125
           LRGYGDSDAPPS+ +YTALHIVGD++G+LD+ GI+KVLLVGHDWGALIAWY CLFRPDRI
Sbjct: 61  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 120

Query: 126 KALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLS 185
           KA V LSVQFFPR+P   FI+GFKA LGDQ YMVRFQEPGKAE EFA+VDIREFFKNV+S
Sbjct: 121 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 186 NRDPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWEL 245
           NRDP APYLP EV FEGVPPPSLAPWLTP+DID YA KF+ +GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 246 TAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQ 305
           TAPWT A+I+VPVKFIVGDLDLTYHFPGA++YING AF+ DVPGLEEV+VI DASHFINQ
Sbjct: 241 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 300

Query: 306 EKPHEINSHIHNFFKKFC 321
           E+P EINSHIH+FF+ FC
Sbjct: 301 ERPVEINSHIHDFFRNFC 318

BLAST of MC08g1683 vs. ExPASy TrEMBL
Match: A0A6J1KD67 (uncharacterized protein LOC111492796 OS=Cucurbita maxima OX=3661 GN=LOC111492796 PE=4 SV=1)

HSP 1 Score: 550 bits (1418), Expect = 2.02e-196
Identity = 250/316 (79.11%), Postives = 284/316 (89.87%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 65
           ME I HTTVKTNG+ +H+AAVG+GPPVLLLHGFPE WYSWRHQ++YL+S GYRAIAPDLR
Sbjct: 1   MEAIHHTTVKTNGINLHIAAVGTGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPDLR 60

Query: 66  GYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 125
           GYGDSDAPPSA AYT LHIVGDL+G+LD+LGIEKVLLVG+DWGA+IAWY CLFRPDRIKA
Sbjct: 61  GYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGNDWGAIIAWYLCLFRPDRIKA 120

Query: 126 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 185
            V LSVQFFPR+PTTPF++ FK+ LGDQ YMVRFQ+PG+AE EF TVDI++FF  V+SNR
Sbjct: 121 AVILSVQFFPRNPTTPFVEAFKSVLGDQFYMVRFQQPGQAEEEFGTVDIKKFFNEVMSNR 180

Query: 186 DPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELTA 245
           DPRAPYLP EV FEG+PPP  A WLTPEDIDVYA  F++TGFTGGLNYYRAFDRTWELTA
Sbjct: 181 DPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADMFSRTGFTGGLNYYRAFDRTWELTA 240

Query: 246 PWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQEK 305
            WT A+I+VPVKFIVGDLDLTYHFPGA+EYI+G  FK DVPGLEEVVV++D SHFINQE+
Sbjct: 241 AWTRAEIKVPVKFIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVVVMKDTSHFINQER 300

Query: 306 PHEINSHIHNFFKKFC 321
           P+E N+HIH+FF KFC
Sbjct: 301 PNETNAHIHDFFNKFC 316

BLAST of MC08g1683 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 431.8 bits (1109), Expect = 4.8e-121
Identity = 190/316 (60.13%), Postives = 242/316 (76.58%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVGSGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLR 65
           ME IEHTT+ TNG+ +HVA++GSGP +L +HGFP+LWYSWRHQ+V  A+ GYRAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 66  GYGDSDAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRIKA 125
           GYGDSDAPPS  +YT LHIVGDL+G+LD LG+++V LVGHDWGA++AW+ C+ RPDR+ A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 126 LVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLSNR 185
           LVN SV F PR+P+   +  F+A  GD  Y+ RFQEPG+ E +FA VD ++      ++R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 186 DPRAPYLPNEVLFEGVP-PPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWELT 245
           +PR P +P  V F G+P PPSL  WLT +D+  Y  KF+Q GFTGGLNYYRA + +WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 246 APWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQE 305
           APWT  +I+VPVKFIVGDLD+TY+ PG +EYI+    K  VP L+EVVV+E   HF++QE
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 306 KPHEINSHIHNFFKKF 321
           KP E+  HI+ FFKKF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of MC08g1683 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 3.4e-106
Identity = 179/323 (55.42%), Postives = 225/323 (69.66%), Query Frame = 0

Query: 6   METIEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPD 65
           ME I+H  V  NG+ +H+A  G   GP VLLLHGFP+LWY+WRHQ+  L+S GYRA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 66  LRGYGDSDAPPSAGAYTALHIVGDLIGVLDDL--GIEKVLLVGHDWGALIAWYFCLFRPD 125
           LRGYGDSD+P S   YT L++VGDL+ +LD +    EKV LVGHDWGA+I W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 126 RIKALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNV 185
           +I   V LSV +  R+P    +QGFKA  GD  Y+ RFQEPGK E E A+ D R F +N+
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 186 LSNRDPRAPYLPNEVLFEGVPPPS-----LAPWLTPEDIDVYAAKFAQTGFTGGLNYYRA 245
            + R    P LP +  F   P P+     L  W + +D+D Y +KF + GFTGGLNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 246 FDRTWELTAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIED 305
            D  WELTAPWT AKI+VPVKF+ GD D+ Y  PG +EYI+G  F  DVP L+E+VVIED
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 306 ASHFINQEKPHEINSHIHNFFKK 320
           A HF+NQEKP E+ +HI++FF K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of MC08g1683 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 359.0 bits (920), Expect = 4.0e-99
Identity = 168/327 (51.38%), Postives = 220/327 (67.28%), Query Frame = 0

Query: 3   QNRMETIEHTTVKTNGVKIHVA-----AVGSGPPVLLLHGFPELWYSWRHQMVYLASAGY 62
           ++R++ +EH T+K NG+ +HVA       G  P +L LHGFPELWY+WRHQMV L+S GY
Sbjct: 48  KSRLDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGY 107

Query: 63  RAIAPDLRGYGDSDAPPSAGAYTALHIVGDLIGVLDDL--GIEKVLLVGHDWGALIAWYF 122
           R IAPDLRGYGD++AP     YT L++ GD++ ++D +  G + V +VGHDWGA+IAW  
Sbjct: 108 RTIAPDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQL 167

Query: 123 CLFRPDRIKALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIR 182
           C +RP+++KALVN+SV F PR+P    +   +   GD  Y+ RFQ+ G+ E EF  +   
Sbjct: 168 CQYRPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTE 227

Query: 183 EFFKNVLSNRDPRAPYLPNEVLFEGV--PPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNY 242
              K  L+ + P    LP +  F+       +L  WLT ED+D Y  K+   GFTG +NY
Sbjct: 228 NVLKEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINY 287

Query: 243 YRAFDRTWELTAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVV 302
           YR  DR WELTAPWT AKIRVPVKFI+GD DLTY+FPGA+EYING  FK DVP L+E VV
Sbjct: 288 YRNIDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVV 347

Query: 303 IEDASHFINQEKPHEINSHIHNFFKKF 321
           ++   HF+++E P  IN HIHNFF KF
Sbjct: 348 LKGLGHFLHEENPDVINQHIHNFFHKF 374

BLAST of MC08g1683 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 350.5 bits (898), Expect = 1.4e-96
Identity = 178/324 (54.94%), Postives = 216/324 (66.67%), Query Frame = 0

Query: 9   IEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLRG 68
           +EH  V+ NG+ IHVA  G   GP VLLLHGFPELWYSWRHQ+  LA+ GYRA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 69  YGDSDAPPSAGAYTALHIVGDLIGVLDDLGI---EKVLLVGHDWGALIAWYFCLFRPDRI 128
           YGDSDAP    +YT  +IVGDLI V+  L     EKV +VGHDWGALIAWY CLFRPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 129 KALVNLSV--QFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNV 188
           KALVNLSV   F P DP+   +   +A  GD  Y+ RFQE G  EAE A V      K +
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 189 LSNRDPRAPYLPNEVLF-----EGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRA 248
           L+ R P    +P +  F     E +P PS   WLT ED+  + +KF + GF+G +NYYR 
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPS---WLTEEDVAYFVSKFEEKGFSGPVNYYRN 240

Query: 249 FDRTWELTAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIED 308
           F+R  EL  PW  +KI+VP KF++G+LDL Y+ PG +EYI+G  FK DVP LEE VV+E 
Sbjct: 241 FNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEG 300

Query: 309 ASHFINQEKPHEINSHIHNFFKKF 321
            +HFINQEKP EI   I +F  KF
Sbjct: 301 VAHFINQEKPQEILQIILDFISKF 321

BLAST of MC08g1683 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.5e-93
Identity = 170/323 (52.63%), Postives = 214/323 (66.25%), Query Frame = 0

Query: 9   IEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPDLRG 68
           +EH  V+ NG+ IHVA  G   G  VLLLHGFPELWYSWRHQ+  LA+ GYRA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 69  YGDSDAPPSAGAYTALHIVGDLIGVLDDLGIE--KVLLVGHDWGALIAWYFCLFRPDRIK 128
           YGDSDAP    ++T  +IVGDL+ V+  L  E  KV +VGHDWGALIAWY CLFRPD++K
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 129 ALVNLSV--QFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVL 188
           ALVNLSV   F+P DP+   +   +A  G+  Y+ RFQE G  EAE A V      K +L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 189 SNRDPRAPYLPNEVLF-----EGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAF 248
           + R P    +P +  F     E +P PS   WLT ED+  + +KF + GF G +NYYR F
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPS---WLTEEDVAYFVSKFKEKGFCGPVNYYRNF 240

Query: 249 DRTWELTAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDA 308
           +R  EL  PW  +KI+VP KF++G+LDL Y+ PG +EYI+G  FK DVP +EE VV+E  
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGV 300

Query: 309 SHFINQEKPHEINSHIHNFFKKF 321
           +HF+NQEKP EI   I +F   F
Sbjct: 301 AHFLNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS04.8e-5740.69Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C25.1e-5137.21Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P349134.4e-5035.83Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349141.0e-4634.42Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
P802995.0e-4632.59Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_022155322.14.35e-23999.68uncharacterized protein LOC111022455 [Momordica charantia][more]
XP_038905173.16.25e-20382.70epoxide hydrolase A-like [Benincasa hispida][more]
XP_022948972.13.74e-19980.06uncharacterized protein LOC111452460 [Cucurbita moschata][more]
XP_023525204.17.21e-19779.11uncharacterized protein LOC111788875 [Cucurbita pepo subsp. pepo][more]
XP_004152243.11.57e-19679.25uncharacterized protein LOC101204575 [Cucumis sativus] >KGN52824.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
A0A6J1DP122.11e-23999.68uncharacterized protein LOC111022455 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1GAQ31.81e-19980.06uncharacterized protein LOC111452460 OS=Cucurbita moschata OX=3662 GN=LOC1114524... [more]
A0A0A0KV847.58e-19779.25AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001... [more]
A0A1S3BXU71.53e-19680.50bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494759 PE... [more]
A0A6J1KD672.02e-19679.11uncharacterized protein LOC111492796 OS=Cucurbita maxima OX=3661 GN=LOC111492796... [more]
Match NameE-valueIdentityDescription
AT4G02340.14.8e-12160.13alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.13.4e-10655.42alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.14.0e-9951.38alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.11.4e-9654.94soluble epoxide hydrolase [more]
AT2G26750.12.5e-9352.63alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 56..71
score: 47.41
coord: 102..115
score: 33.25
coord: 116..129
score: 26.85
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 30..136
e-value: 2.0E-23
score: 83.4
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 56..71
score: 63.64
coord: 116..129
score: 49.35
coord: 294..316
score: 38.93
coord: 102..115
score: 55.52
coord: 254..270
score: 38.77
coord: 36..54
score: 59.33
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 6..320
e-value: 1.2E-111
score: 375.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..319
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 6..320
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 6..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1683.1MC08g1683.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity