MC08g1675 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1675
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionFumarate hydratase
LocationMC08: 24740012 .. 24745657 (+)
RNA-Seq ExpressionMC08g1675
SyntenyMC08g1675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCGTTTTGATGGGCCGCCTGAATGGGCCGAAGGGAAGAAAAGTCCGGTCCGAGGCAGGCGCAGCCATGATTATGAAAGCCATTCCACTTTCACTTTCACATTTCAACATTTTCCGACAAAAGGAAATCGCCGTATTCTCTCAGAACTTCCTCTGAAAGCTCTAACGTCACCTGCCATCTCTCCGGCGCCTAGCGCCTTATCGCCGCCCATTTCTCCCTTGAGGATTCCACCTTCCGGACCTCCATCGTTAATCTCCTCTTCTACTCGCAAGATCACTCGCTCATTCTTCTTGCCAGAAGAATGGCTGCCGCGTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTCGGGATGGCGCGTTGTGCTACTGCGTCTTTGAGATCTTACTCCACGTCTTTTAGGGAGGAAAGAGATACGTTTGGACCGATTAACGTTCCCTCCGACAGGTTCGTGTGTGTCTCTCTCTCTCTCGTTCATCTGGTTTTTGGATTTGCTTGACTAACACTTGCTTTTGTTTTCCATTTCCTCAAAGGAAATTGTGATAAATGAAGTGAAATAAGTATTTTAATTACGTTGTTTGCCTTTTTAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTCGAGATCGGAGGAGACCGTGAGCGGATGCCTGAACCTATAGTTCGTGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTCCATTCGTTTTCAAATTAAGTTATAATGTGATGTTTGATTTTTTGTGCTACAGTTCGTATTTTGCAATTGAGATGAGATATTATTGCTTGGAAATGGTTCTACTGTTCATATTGAATCTGTTTTTGTAGGTAAACATGGAATATGGTCTTGATCCAACTATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCCGAGGGAAAGTTAAATGATCACTTTCCGCTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCCAATGAGGTTAGGAGAACTACTCTGTTTTATCTTGATTGTGAGGTGCCAAGAGCGAAGGTTCAATCTATTAAGGATCTTATTGAAAGTCTTACATATTTCTTTCTTTTACATGAATCTCTTTCTTTGATCACGTTGTCGTGGTATTTTTTACCCAAGGAAGCATGAAAGGCTAGGTCACTGTTTTTATGTTAGGTTTGAGTTCTTATTAACGGCAGATGTGATGGCATGTGGTATTTAATCCCTTAATATACAACTCTCCCATGGGAGAGATTTGCTTTCCTTGGAAAATTAGGTTCCGTCAATCGCCATTCTTGAATCTAATGCAAAGTTCAAATCTAATTATTTCCTGAACTCCATGGCTTAAATTTATAATGCCATGTACGTGAAGTATGCGGCTTCCAAACTAGTGGTTACTGATGGTTGAATTGAAGACGTTGTAAGATGACTACAGTTTTTTTCACCCCCTCCCTTCCCTATGTAGTCAAATTCACCGAGTCTTCCTAAAGTACAGTCTCTTGAGAACAGAAAAGCATTTCCTCCAATAAGATATACATTTCCCTTCAATAATATTTGAAGTATCTAATATGAATGTTATGCGCACATGATTGCAGAACATAGGGTAATATGTTGAGTAATCTTCTTATAACGTCTCTGAGGGTATATTTTGTTAGTTGATTACTTGAATGGTACAAAATTGTGGACATACTATTTTTGTGGCAGGTCATTGCTAACAGAGCATCAGAGATCCTTGGCCATAAACGTGGTGAGAAGTTTGTGCACCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTTAGTCATTTGCTTTCTTAAGTTATTTTGTGTATTGTTATTCCAACTCTTTTTCTGTAAATAACCAATAAAGTTTAGCAGTTTTCCATGGCAAAACTTCTTTTTTCCATAGAAGTTTTAATGATCAAAGTTTATGCCATTACTACTTTGGTCAAATTTTTATGCCATTACTACTTTTATTTGTCTCAATTGACAAGAGTTTTTTCCTCACTTCTGATGATTTCTACTGATTTCCTTTTACAGGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCATACCAAAATTGAAAACTTTGCATTCATCACTAAACTCAAAGGTTTGATTTTTATTAGAGATACGATGCCCAGAATTTCAAATTTTTGTTTGCATCTTTACATGTTTTAAATAATCCGACCTTAATTTATTTACAGAAGCCTGTTTGAGTCATAATCTTAGGTCATGTTTGTTTGATTGCATGGATTAAGCTGAATGTCTAAAAAGCATGGAAAGCATTAGGAGATCATAACATAGTTTGAATCTGATTTACCATCTGTTAGTTTGAACACTTCTTAACTGAGTTGTATGGTAGTGATTGTATGTGTTTATTTACACTCCCTGCTCCTTTGCATTTTATTTGCCCTTTGCACCTTAATTTAAGTCTGTAATTCCTTATATTACTCTGTTATGATCCAAACAGTCCATTGAATTCAAAGATATTGTTAAGATTGGACGCACTCACACTCAAGATGCCACACCATTAACCCTTGGGCAAGAGTTCAGCGGCTATACCACACAAGTATGCTTTCTTTCTCTTTGTGGTTGTGAAAGTTTTGGTGGTCCTGATGCCTATTGAAAAGAAGAAATAGAGTACGATGCATATAAATAATTTTCTGATTGGTGTGTGCTTGGGCTGCAGGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGGTTGTGATGCTTGTAATTGTTCCAGGTATTTTTTCAATTCCTTTTGCTATTTTTATTTTTGACACTTCAGAATTCCATTTGTACAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGGTATATTTTCTTTCGAATTAGTATGAATAAGAAAAAATATCTAGTTTTCTTATATTGTGCTTCAAGACCAGACGGATGAGCGTATTTCTTATTTTGAGATTGGCGCTGTCTATTATTCAGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAATTTCCCGTTTGTGACGGCAGAAAACAAGTTTGAAGCTCTGGTTAGTCAATTGAAGGAGCATCATCTTCCCTCTCTTTTGTTTTTTATTGCACAGTTTGGTGTTGCCTTCCTTCATACATGGAACCATTTTTTTATGTTGGAATATTTTGTTTTGCTAGGCGGCACATGACGCTTTTGTTGAGACTAGTGGGGCTCTTAACACAGTTGCTACTTCCTTGATGAAGATTGCTAATGACATACGCCTGTTGGGAAGGTTTATATGCATTAATTTCGTCGATCCTAGCACTGTATCATTTCTTGTCTTTCTTTATCTAATATTTCCCCCCTCATTTCATGTATACAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAATGAGCCAGGCAGTAGTATTATGCCAGTAAATCTCTCTGTCTCTCTCTCTGTGTTTTGGGGGCACATGCGTGTCTGTCTGTCTTTCAGAAGCTATGTTTCAATGCTTTGGGCTTGTTAAGCTTAACAATTTTTTCTGGTTCTAGGGGAAGGTCAACCCTACTCAGTGCGAGGCTCTCACTATGGTCTGTGCTCAGGTATAGTTACCTTCTAGCATAGTTCATTATGTGATGCTGAAAGAGGAATTAGTTAATATAGATCCAACTAATTATGTTGGTTTCCATTTGTTCCCCAAAAATTTTGAGTTACCATTCTAAGCTATGCATACTCTGGTTATCAGTGACTAGTTTCATATATGTAATATATTGTCATTCTTGAACCTTAATTAGTTCAATGTGTTCCAACAAATTTTTGTTAATATTTTGTTTATTTGTTTTAAATAAATCAATTAAAAGTTTGTTTCTAATTTTTTAATTAAAAAAAAATATATATTAGATGCGATCGACAGTTCTTCATGTTTTACAGGTTATGGGAAACCATGTGGCAGTAACGGTTGGTGGATCAAATGGACACTTTGAGCTTAATGTATTTAAGCCAATGATCGCCAATGCTCTTTTGCATGTAAGTTCTGTAGTATCTCTTTATCGTTGTTATTTCTAGATATTATGTTTAAATAATGATATCATAAACTAAACTATTATCTTGTGGTTGCACCATCTGCTATGAGATTCATTTATCATATTGTCGATCACTCGATCTGCTTATCGTCCTCAAATGACATGCACGCACGCACATATATATATAGAGTTAGAAATAAATATTCTGGATTTATTGAACCTTCATCTCGAACTCTGGCTCTGCAGTCATTGAGATTATTAGGAGATGCTTCTGCTTCATTTGAAAAGAACTGTGTAAGAGGAATTCAAGCTAACACGGAAAGAATTTCGAAATTATTGCGTGAGGTTAGTTTTATGTTAATATTTGAAAAAGATAATTTTATGCTACACCGCTTGTGTTGAAGATTTTGGCTGATGATGATTCTTGTGTATATCTCTGCACTTAAATTCGATGGCCTTGCAGTCGCTGATGCTCGTCACAGCTTTGAACCCTGTAAGTGATCATAACTAGAAAGCTCACATTTTCCTTAGGCTTGGAACATTGTTTGATAACGTGTTCATGCATACTGTTTCCAGAAAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCCCACAAGGAAGGATCCACTTTGAAGGTATGAATTGCGTTTGAATTACATTTATAATCCTAATTGTATAGGTTTAATCTTATTTTCATCTCTAAACATTTCATGCTTATACTGTTTCTAGTCTTTAAACTTTCAAACATTTTTTTTTATCTCTAAAGTTGTGCATAAAGAACTATTTAGTCAAAGTCACTCATTTTTAAATAAATTAGTTAATAGAAGTTTGATATGGGTATCTCCTTATTCATTAGCCTAATCAATAGGCATCTGTAAGTCATGTGTTGTCAGTTGGGTGTCAAGTTTTCAAATGCATTTAGGTTCTTGTTAAATGATTTAGGGAAATTCAAATGGTAAGCACATTAGATTGTCTGGACTAAAAATAGGACATTTGTAAGTTTAGAGAATATATAAATACACAACCAAAATAGGATTTAAACTCATATATCTTTTTTTAGTACAACATGTGGATAGAGGATCGAACTCACAATCTCTTGGTTGGAGATATATGCCAATTACCGCTGAGCTAGCTTTGGTCCATATTTTTATTATTACCTAAATTGTCAAATGGCTCGTTTTGGTATATAATTTTGTAGGACGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGAGTTTGATAAACATGTGGTGCCTGAGAAGATGATTGGTCCATCTGATTGATAAAAAGTTGTTTTGTGTTTGGCTCATTGTTTCATCAAAATAATTTTGATAGGTACTTTCATTTTTCTCCTAAAAGGAAGAAGAAATCCATTTTTATGAGGATTGAACTGGATAATAAAAAGACCACATATAATAGGATCTCTCCTAAGGATTGTGGTCGTTCCTCTTGTATTTTCTAGAAAATTCTCTCACCGGAAGTAATTAATAATTTATTTTGGATACATTTTTTGTGGTACGTTGATTATTGCATCTCTTTGGTGCGTTTTTCTTATAAATGGTGGAT

mRNA sequence

GGCGTTTTGATGGGCCGCCTGAATGGGCCGAAGGGAAGAAAAGTCCGGTCCGAGGCAGGCGCAGCCATGATTATGAAAGCCATTCCACTTTCACTTTCACATTTCAACATTTTCCGACAAAAGGAAATCGCCGTATTCTCTCAGAACTTCCTCTGAAAGCTCTAACGTCACCTGCCATCTCTCCGGCGCCTAGCGCCTTATCGCCGCCCATTTCTCCCTTGAGGATTCCACCTTCCGGACCTCCATCGTTAATCTCCTCTTCTACTCGCAAGATCACTCGCTCATTCTTCTTGCCAGAAGAATGGCTGCCGCGTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTCGGGATGGCGCGTTGTGCTACTGCGTCTTTGAGATCTTACTCCACGTCTTTTAGGGAGGAAAGAGATACGTTTGGACCGATTAACGTTCCCTCCGACAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTCGAGATCGGAGGAGACCGTGAGCGGATGCCTGAACCTATAGTTCGTGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTAAACATGGAATATGGTCTTGATCCAACTATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCCGAGGGAAAGTTAAATGATCACTTTCCGCTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCCAATGAGGTCATTGCTAACAGAGCATCAGAGATCCTTGGCCATAAACGTGGTGAGAAGTTTGTGCACCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCATACCAAAATTGAAAACTTTGCATTCATCACTAAACTCAAAGTCCATTGAATTCAAAGATATTGTTAAGATTGGACGCACTCACACTCAAGATGCCACACCATTAACCCTTGGGCAAGAGTTCAGCGGCTATACCACACAAGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAATTTCCCGTTTGTGACGGCAGAAAACAAGTTTGAAGCTCTGGCGGCACATGACGCTTTTGTTGAGACTAGTGGGGCTCTTAACACAGTTGCTACTTCCTTGATGAAGATTGCTAATGACATACGCCTGTTGGGAAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAATGAGCCAGGCAGTAGTATTATGCCAGGGAAGGTCAACCCTACTCAGTGCGAGGCTCTCACTATGGTCTGTGCTCAGGTTATGGGAAACCATGTGGCAGTAACGGTTGGTGGATCAAATGGACACTTTGAGCTTAATGTATTTAAGCCAATGATCGCCAATGCTCTTTTGCATTCATTGAGATTATTAGGAGATGCTTCTGCTTCATTTGAAAAGAACTGTGTAAGAGGAATTCAAGCTAACACGGAAAGAATTTCGAAATTATTGCGTGAGTCGCTGATGCTCGTCACAGCTTTGAACCCTAAAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCCCACAAGGAAGGATCCACTTTGAAGGACGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGAGTTTGATAAACATGTGGTGCCTGAGAAGATGATTGGTCCATCTGATTGATAAAAAGTTGTTTTGTGTTTGGCTCATTGTTTCATCAAAATAATTTTGATAGGTACTTTCATTTTTCTCCTAAAAGGAAGAAGAAATCCATTTTTATGAGGATTGAACTGGATAATAAAAAGACCACATATAATAGGATCTCTCCTAAGGATTGTGGTCGTTCCTCTTGTATTTTCTAGAAAATTCTCTCACCGGAAGTAATTAATAATTTATTTTGGATACATTTTTTGTGGTACGTTGATTATTGCATCTCTTTGGTGCGTTTTTCTTATAAATGGTGGAT

Coding sequence (CDS)

ATGGCTGCCGCGTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTCGGGATGGCGCGTTGTGCTACTGCGTCTTTGAGATCTTACTCCACGTCTTTTAGGGAGGAAAGAGATACGTTTGGACCGATTAACGTTCCCTCCGACAGATTATGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTCGAGATCGGAGGAGACCGTGAGCGGATGCCTGAACCTATAGTTCGTGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTAAACATGGAATATGGTCTTGATCCAACTATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCCGAGGGAAAGTTAAATGATCACTTTCCGCTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCCAATGAGGTCATTGCTAACAGAGCATCAGAGATCCTTGGCCATAAACGTGGTGAGAAGTTTGTGCACCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCATACCAAAATTGAAAACTTTGCATTCATCACTAAACTCAAAGTCCATTGAATTCAAAGATATTGTTAAGATTGGACGCACTCACACTCAAGATGCCACACCATTAACCCTTGGGCAAGAGTTCAGCGGCTATACCACACAAGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAATTTCCCGTTTGTGACGGCAGAAAACAAGTTTGAAGCTCTGGCGGCACATGACGCTTTTGTTGAGACTAGTGGGGCTCTTAACACAGTTGCTACTTCCTTGATGAAGATTGCTAATGACATACGCCTGTTGGGAAGTGGTCCACGTTGTGGTCTTGGTGAACTTATTCTTCCCGAAAATGAGCCAGGCAGTAGTATTATGCCAGGGAAGGTCAACCCTACTCAGTGCGAGGCTCTCACTATGGTCTGTGCTCAGGTTATGGGAAACCATGTGGCAGTAACGGTTGGTGGATCAAATGGACACTTTGAGCTTAATGTATTTAAGCCAATGATCGCCAATGCTCTTTTGCATTCATTGAGATTATTAGGAGATGCTTCTGCTTCATTTGAAAAGAACTGTGTAAGAGGAATTCAAGCTAACACGGAAAGAATTTCGAAATTATTGCGTGAGTCGCTGATGCTCGTCACAGCTTTGAACCCTAAAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCCCACAAGGAAGGATCCACTTTGAAGGACGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGAGTTTGATAAACATGTGGTGCCTGAGAAGATGATTGGTCCATCTGATTGA

Protein sequence

MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGPSD
Homology
BLAST of MC08g1675 vs. ExPASy Swiss-Prot
Match: P93033 (Fumarate hydratase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FUM1 PE=1 SV=2)

HSP 1 Score: 862.1 bits (2226), Expect = 3.1e-249
Identity = 438/490 (89.39%), Postives = 456/490 (93.06%), Query Frame = 0

Query: 5   YVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFE 64
           YV SRR S        R AT SLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQNFE
Sbjct: 4   YVASRRLSGGTTVTALRYAT-SLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQNFE 63

Query: 65  IGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 124
           IGG+RERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV
Sbjct: 64  IGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 123

Query: 125 WQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAAT 184
           WQTGSGTQSNMNANEVIANRA+EILG KRGEK VHPNDHVNRSQSSNDTFPTVMHIAAAT
Sbjct: 124 WQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAAAT 183

Query: 185 EINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDR 244
           EINSR IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG++R
Sbjct: 184 EINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGLNR 243

Query: 245 VLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAF 304
           V CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA 
Sbjct: 244 VTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAC 303

Query: 305 VETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALT 364
           VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEALT
Sbjct: 304 VETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEALT 363

Query: 365 MVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQAN 424
           MVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN
Sbjct: 364 MVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIEAN 423

Query: 425 TERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHV 484
            ERISKLL ESLMLVT+LNPKIGYDNAAAVAK AHKEG TLK+AAL LGVL+ EEFD  V
Sbjct: 424 RERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDTLV 483

Query: 485 VPEKMIGPSD 495
           VPEKMIGPSD
Sbjct: 484 VPEKMIGPSD 492

BLAST of MC08g1675 vs. ExPASy Swiss-Prot
Match: Q9FI53 (Fumarate hydratase 2 OS=Arabidopsis thaliana OX=3702 GN=FUM2 PE=1 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 2.3e-244
Identity = 422/467 (90.36%), Postives = 441/467 (94.43%), Query Frame = 0

Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIE 207
           ILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRL 327
           TKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVT+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLRESLMLVTALNPKIG 447
           LNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN ERISKLL ESLMLVT+LNPKIG
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIG 452

Query: 448 YDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGPSD 495
           YDNAAAVAK AHKEG TLK AA+KLGVL+ EEFD  VVPEKMIGPSD
Sbjct: 453 YDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGPSD 499

BLAST of MC08g1675 vs. ExPASy Swiss-Prot
Match: P14408 (Fumarate hydratase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fh PE=1 SV=2)

HSP 1 Score: 671.8 bits (1732), Expect = 6.0e-192
Identity = 338/476 (71.01%), Postives = 392/476 (82.35%), Query Frame = 0

Query: 19  MARCATASLRSYS-TSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIV 78
           + RCA   +R  S  SFR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P++
Sbjct: 31  LPRCAPNVVRMASQNSFRIEYDTFGELKVPTDKYYGAQTVRSTMNFKIGGATERMPIPVI 90

Query: 79  RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNA 138
           +AFG+LK+ AA+VN EYGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN 
Sbjct: 91  KAFGILKRAAAEVNQEYGLDPKIASAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNV 150

Query: 139 NEVIANRASEILGHKRG-EKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKT 198
           NEVI+NRA E+LG + G +K VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ 
Sbjct: 151 NEVISNRAIEMLGGELGSKKPVHPNDHVNKSQSSNDTFPTAMHIAAALEVHQVLLPGLQK 210

Query: 199 LHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLA 258
           LH +L++KS EF  ++KIGRTHTQDA PLTLGQEFSGY  QV+Y ++R+   +PR+Y+LA
Sbjct: 211 LHDALSAKSKEFAQVIKIGRTHTQDAVPLTLGQEFSGYVQQVQYAMERIKAAMPRIYELA 270

Query: 259 QGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVAT 318
            GGTAVGTGLNT+ GF  K+AA VA  T  PFVTA NKFEALAAHDA VE SGA+NT A 
Sbjct: 271 AGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTAC 330

Query: 319 SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 378
           SLMKIANDIR LGSGPR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVA
Sbjct: 331 SLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVA 390

Query: 379 VTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLRESL 438
           VTVGGSNGHFELNVFKPM+   +LHS RLLGDAS SF +NCV GIQANTERI+KL+ ESL
Sbjct: 391 VTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESL 450

Query: 439 MLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGP 493
           MLVTALNP IGYD AA +AKTAHK GSTLK+ A++LG L+ E+FD+ V P+ M+GP
Sbjct: 451 MLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 506

BLAST of MC08g1675 vs. ExPASy Swiss-Prot
Match: Q60HF9 (Fumarate hydratase, mitochondrial OS=Macaca fascicularis OX=9541 GN=FH PE=2 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 1.0e-191
Identity = 335/469 (71.43%), Postives = 386/469 (82.30%), Query Frame = 0

Query: 25  ASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLK 84
           A+  +   SFR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+++AFG+LK
Sbjct: 41  AARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILK 100

Query: 85  KCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANR 144
           + AA+VN +YGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NEVI+NR
Sbjct: 101 RAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNR 160

Query: 145 ASEILGHKRGEKF-VHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNS 204
           A E+LG + G K  VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ LH +L++
Sbjct: 161 AIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDA 220

Query: 205 KSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVG 264
           KS EF  I+KIGRTHTQDA PLTLGQEFSGY  QVKY + R+   +PR+Y+LA GGTAVG
Sbjct: 221 KSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAVTRIKAAMPRIYELAAGGTAVG 280

Query: 265 TGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIAN 324
           TGLNT+ GF  K+AA VA  T  PFVTA NKFEALAAHDA VE SGA+NT A SLMKIAN
Sbjct: 281 TGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIAN 340

Query: 325 DIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSN 384
           DIR LGSGPR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVAVTVGGSN
Sbjct: 341 DIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSN 400

Query: 385 GHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLRESLMLVTALN 444
           GHFELNVFKPM+   +LHS RLLGDAS SF +NCV GIQANTERI+KL+ ESLMLVTALN
Sbjct: 401 GHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALN 460

Query: 445 PKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGP 493
           P IGYD AA +AKTAHK GSTLK+ A++LG L+ E+FD+ V P+ M+GP
Sbjct: 461 PHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 509

BLAST of MC08g1675 vs. ExPASy Swiss-Prot
Match: P07954 (Fumarate hydratase, mitochondrial OS=Homo sapiens OX=9606 GN=FH PE=1 SV=3)

HSP 1 Score: 670.2 bits (1728), Expect = 1.7e-191
Identity = 335/469 (71.43%), Postives = 386/469 (82.30%), Query Frame = 0

Query: 25  ASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLK 84
           A+  +   SFR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+++AFG+LK
Sbjct: 41  AARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILK 100

Query: 85  KCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANR 144
           + AA+VN +YGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NEVI+NR
Sbjct: 101 RAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNR 160

Query: 145 ASEILGHKRGEKF-VHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNS 204
           A E+LG + G K  VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ LH +L++
Sbjct: 161 AIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDA 220

Query: 205 KSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVG 264
           KS EF  I+KIGRTHTQDA PLTLGQEFSGY  QVKY + R+   +PR+Y+LA GGTAVG
Sbjct: 221 KSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVG 280

Query: 265 TGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIAN 324
           TGLNT+ GF  K+AA VA  T  PFVTA NKFEALAAHDA VE SGA+NT A SLMKIAN
Sbjct: 281 TGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIAN 340

Query: 325 DIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSN 384
           DIR LGSGPR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVAVTVGGSN
Sbjct: 341 DIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSN 400

Query: 385 GHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLRESLMLVTALN 444
           GHFELNVFKPM+   +LHS RLLGDAS SF +NCV GIQANTERI+KL+ ESLMLVTALN
Sbjct: 401 GHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALN 460

Query: 445 PKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGP 493
           P IGYD AA +AKTAHK GSTLK+ A++LG L+ E+FD+ V P+ M+GP
Sbjct: 461 PHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 509

BLAST of MC08g1675 vs. NCBI nr
Match: XP_022155265.1 (fumarate hydratase 1, mitochondrial [Momordica charantia])

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF
Sbjct: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480

Query: 481 DKHVVPEKMIGPSD 494
           DKHVVPEKMIGPSD
Sbjct: 481 DKHVVPEKMIGPSD 494

BLAST of MC08g1675 vs. NCBI nr
Match: XP_022984048.1 (fumarate hydratase 1, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 949 bits (2454), Expect = 0.0
Identity = 480/494 (97.17%), Postives = 488/494 (98.79%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFGMAR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFLTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQAN ERISKLL ESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLK+AALKLGVLSEE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSD 494
           +K+VVPEKMIGPSD
Sbjct: 481 NKYVVPEKMIGPSD 494

BLAST of MC08g1675 vs. NCBI nr
Match: XP_023528894.1 (fumarate hydratase 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 946 bits (2445), Expect = 0.0
Identity = 478/494 (96.76%), Postives = 486/494 (98.38%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFGMAR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFLTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDAS SFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASGSFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQAN ERISKLL ESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLK+AALKLGVLSEE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSD 494
           +K+VVPEKMIGPSD
Sbjct: 481 NKYVVPEKMIGPSD 494

BLAST of MC08g1675 vs. NCBI nr
Match: XP_004152237.1 (fumarate hydratase 1, mitochondrial [Cucumis sativus] >KGN52832.1 hypothetical protein Csa_014636 [Cucumis sativus])

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 475/494 (96.15%), Postives = 484/494 (97.98%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSS SHLFGMAR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQAN ERISKLL ESLMLVTALNPKIGYDNAAAVAK AHKEGSTLK+AALKLGVLSEE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSD 494
           DKHVVPEKMIGPSD
Sbjct: 481 DKHVVPEKMIGPSD 494

BLAST of MC08g1675 vs. NCBI nr
Match: KAA0044396.1 (fumarate hydratase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 475/493 (96.35%), Postives = 482/493 (97.77%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFGMAR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPT+GKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTVGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQAN ERISKLL ESLMLVTALNPKIGYDNAAAVAK AHKEG TLK+AALKLGVL EEEF
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEEF 480

Query: 481 DKHVVPEKMIGPS 493
           DKHVVPEKMIGPS
Sbjct: 481 DKHVVPEKMIGPS 493

BLAST of MC08g1675 vs. ExPASy TrEMBL
Match: A0A6J1DLZ0 (Fumarate hydratase OS=Momordica charantia OX=3673 GN=LOC111022407 PE=3 SV=1)

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF
Sbjct: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480

Query: 481 DKHVVPEKMIGPSD 494
           DKHVVPEKMIGPSD
Sbjct: 481 DKHVVPEKMIGPSD 494

BLAST of MC08g1675 vs. ExPASy TrEMBL
Match: A0A6J1J9G0 (Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482474 PE=3 SV=1)

HSP 1 Score: 949 bits (2454), Expect = 0.0
Identity = 480/494 (97.17%), Postives = 488/494 (98.79%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFGMAR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPF+TAENKFEALAA
Sbjct: 241 GIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFLTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQAN ERISKLL ESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLK+AALKLGVLSEE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSD 494
           +K+VVPEKMIGPSD
Sbjct: 481 NKYVVPEKMIGPSD 494

BLAST of MC08g1675 vs. ExPASy TrEMBL
Match: A0A5A7TQL4 (Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001250 PE=3 SV=1)

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 475/493 (96.35%), Postives = 482/493 (97.77%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFGMAR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPT+GKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTVGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQAN ERISKLL ESLMLVTALNPKIGYDNAAAVAK AHKEG TLK+AALKLGVL EEEF
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEEF 480

Query: 481 DKHVVPEKMIGPS 493
           DKHVVPEKMIGPS
Sbjct: 481 DKHVVPEKMIGPS 493

BLAST of MC08g1675 vs. ExPASy TrEMBL
Match: A0A0A0KWM0 (Fumarate hydratase OS=Cucumis sativus OX=3659 GN=Csa_4G001990 PE=3 SV=1)

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 475/494 (96.15%), Postives = 484/494 (97.98%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSS SHLFGMAR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRG+KFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGDKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQAN ERISKLL ESLMLVTALNPKIGYDNAAAVAK AHKEGSTLK+AALKLGVLSEE+F
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAALKLGVLSEEDF 480

Query: 481 DKHVVPEKMIGPSD 494
           DKHVVPEKMIGPSD
Sbjct: 481 DKHVVPEKMIGPSD 494

BLAST of MC08g1675 vs. ExPASy TrEMBL
Match: A0A5D3E117 (Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001270 PE=3 SV=1)

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 475/493 (96.35%), Postives = 482/493 (97.77%), Query Frame = 0

Query: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFGMAR AT SLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180
           PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 121 PLVVWQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 180

Query: 181 AAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240
           AAATEINS+FIPKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY
Sbjct: 181 AAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 240

Query: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAA 300
           GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PF+TAENKFEALAA
Sbjct: 241 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFMTAENKFEALAA 300

Query: 301 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360
           HDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC
Sbjct: 301 HDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 360

Query: 361 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 420
           EALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDASASFEKNCVRG
Sbjct: 361 EALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDASASFEKNCVRG 420

Query: 421 IQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEF 480
           IQAN ERISKLL ESLMLVTALNPKIGYDNAAAVAK AHKEG TLK+AALKLGVL EEEF
Sbjct: 421 IQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGCTLKEAALKLGVLCEEEF 480

Query: 481 DKHVVPEKMIGPS 493
           DKHVVPEKMIGPS
Sbjct: 481 DKHVVPEKMIGPS 493

BLAST of MC08g1675 vs. TAIR 10
Match: AT2G47510.1 (fumarase 1 )

HSP 1 Score: 862.1 bits (2226), Expect = 2.2e-250
Identity = 438/490 (89.39%), Postives = 456/490 (93.06%), Query Frame = 0

Query: 5   YVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFE 64
           YV SRR S        R AT SLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQNFE
Sbjct: 4   YVASRRLSGGTTVTALRYAT-SLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQNFE 63

Query: 65  IGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 124
           IGG+RERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV
Sbjct: 64  IGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 123

Query: 125 WQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAAT 184
           WQTGSGTQSNMNANEVIANRA+EILG KRGEK VHPNDHVNRSQSSNDTFPTVMHIAAAT
Sbjct: 124 WQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAAAT 183

Query: 185 EINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDR 244
           EINSR IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG++R
Sbjct: 184 EINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGLNR 243

Query: 245 VLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAF 304
           V CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA 
Sbjct: 244 VTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAC 303

Query: 305 VETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALT 364
           VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEALT
Sbjct: 304 VETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEALT 363

Query: 365 MVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQAN 424
           MVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN
Sbjct: 364 MVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIEAN 423

Query: 425 TERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHV 484
            ERISKLL ESLMLVT+LNPKIGYDNAAAVAK AHKEG TLK+AAL LGVL+ EEFD  V
Sbjct: 424 RERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDTLV 483

Query: 485 VPEKMIGPSD 495
           VPEKMIGPSD
Sbjct: 484 VPEKMIGPSD 492

BLAST of MC08g1675 vs. TAIR 10
Match: AT2G47510.2 (fumarase 1 )

HSP 1 Score: 862.1 bits (2226), Expect = 2.2e-250
Identity = 438/490 (89.39%), Postives = 456/490 (93.06%), Query Frame = 0

Query: 5   YVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFE 64
           YV SRR S        R AT SLRSYSTSFREERDTFGPI VPSD+LWGAQTQRSLQNFE
Sbjct: 4   YVASRRLSGGTTVTALRYAT-SLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQNFE 63

Query: 65  IGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 124
           IGG+RERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV
Sbjct: 64  IGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVV 123

Query: 125 WQTGSGTQSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAAT 184
           WQTGSGTQSNMNANEVIANRA+EILG KRGEK VHPNDHVNRSQSSNDTFPTVMHIAAAT
Sbjct: 124 WQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNRSQSSNDTFPTVMHIAAAT 183

Query: 185 EINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDR 244
           EINSR IP LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQVKYG++R
Sbjct: 184 EINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGLNR 243

Query: 245 VLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAF 304
           V CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA 
Sbjct: 244 VTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDAC 303

Query: 305 VETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALT 364
           VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIMPGKVNPTQCEALT
Sbjct: 304 VETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEALT 363

Query: 365 MVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQAN 424
           MVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN
Sbjct: 364 MVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIEAN 423

Query: 425 TERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHV 484
            ERISKLL ESLMLVT+LNPKIGYDNAAAVAK AHKEG TLK+AAL LGVL+ EEFD  V
Sbjct: 424 RERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDTLV 483

Query: 485 VPEKMIGPSD 495
           VPEKMIGPSD
Sbjct: 484 VPEKMIGPSD 492

BLAST of MC08g1675 vs. TAIR 10
Match: AT5G50950.2 (FUMARASE 2 )

HSP 1 Score: 845.9 bits (2184), Expect = 1.7e-245
Identity = 422/467 (90.36%), Postives = 441/467 (94.43%), Query Frame = 0

Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIE 207
           ILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRL 327
           TKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVT+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLRESLMLVTALNPKIG 447
           LNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN ERISKLL ESLMLVT+LNPKIG
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIG 452

Query: 448 YDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIGPSD 495
           YDNAAAVAK AHKEG TLK AA+KLGVL+ EEFD  VVPEKMIGPSD
Sbjct: 453 YDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGPSD 499

BLAST of MC08g1675 vs. TAIR 10
Match: AT5G50950.1 (FUMARASE 2 )

HSP 1 Score: 805.8 bits (2080), Expect = 1.9e-233
Identity = 404/455 (88.79%), Postives = 424/455 (93.19%), Query Frame = 0

Query: 28  RSYSTSFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRASE 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRA+E
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVIANRAAE 152

Query: 148 ILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIE 207
           ILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR IP LK LHSSL SKS E
Sbjct: 153 ILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSLESKSFE 212

Query: 208 FKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLN 267
           FKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLAQGGTAVGTGLN
Sbjct: 213 FKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLAQGGTAVGTGLN 272

Query: 268 TKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRL 327
           TKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR 
Sbjct: 273 TKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIATSLMKIANDIRF 332

Query: 328 LGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFE 387
           LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVT+GGSNGHFE
Sbjct: 333 LGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTIGGSNGHFE 392

Query: 388 LNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKLLRESLMLVTALNPKIG 447
           LNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN ERISKLL ESLMLVT+LNPKIG
Sbjct: 393 LNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIG 452

Query: 448 YDNAAAVAKTAHKEGSTLK--DAALKLGVLSEEEF 481
           YDNAAAVAK AHKEG TLK  +  L    L++ EF
Sbjct: 453 YDNAAAVAKRAHKEGCTLKVNNKLLTFSSLNKSEF 487

BLAST of MC08g1675 vs. TAIR 10
Match: AT5G50950.3 (FUMARASE 2 )

HSP 1 Score: 760.8 bits (1963), Expect = 7.0e-220
Identity = 381/423 (90.07%), Postives = 399/423 (94.33%), Query Frame = 0

Query: 72  MPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGT 131
           MPEPIVRAFGVLKKCAAKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGT
Sbjct: 1   MPEPIVRAFGVLKKCAAKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGT 60

Query: 132 QSNMNANEVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFI 191
           QSNMNANEVIANRA+EILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR I
Sbjct: 61  QSNMNANEVIANRAAEILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLI 120

Query: 192 PKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPR 251
           P LK LHSSL SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR
Sbjct: 121 PSLKNLHSSLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPR 180

Query: 252 MYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFVTAENKFEALAAHDAFVETSGAL 311
           +YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETN PFVTAENKFEALAAHDA VETSG+L
Sbjct: 181 IYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSL 240

Query: 312 NTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVM 371
           NT+ATSLMKIANDIR LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVM
Sbjct: 241 NTIATSLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVM 300

Query: 372 GNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANTERISKL 431
           GNHVAVT+GGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN ERISKL
Sbjct: 301 GNHVAVTIGGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKL 360

Query: 432 LRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAALKLGVLSEEEFDKHVVPEKMIG 491
           L ESLMLVT+LNPKIGYDNAAAVAK AHKEG TLK AA+KLGVL+ EEFD  VVPEKMIG
Sbjct: 361 LHESLMLVTSLNPKIGYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIG 420

Query: 492 PSD 495
           PSD
Sbjct: 421 PSD 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P930333.1e-24989.39Fumarate hydratase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FUM1 PE=1... [more]
Q9FI532.3e-24490.36Fumarate hydratase 2 OS=Arabidopsis thaliana OX=3702 GN=FUM2 PE=1 SV=1[more]
P144086.0e-19271.01Fumarate hydratase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fh PE=1 SV=2[more]
Q60HF91.0e-19171.43Fumarate hydratase, mitochondrial OS=Macaca fascicularis OX=9541 GN=FH PE=2 SV=1[more]
P079541.7e-19171.43Fumarate hydratase, mitochondrial OS=Homo sapiens OX=9606 GN=FH PE=1 SV=3[more]
Match NameE-valueIdentityDescription
XP_022155265.10.0100.00fumarate hydratase 1, mitochondrial [Momordica charantia][more]
XP_022984048.10.097.17fumarate hydratase 1, mitochondrial-like isoform X2 [Cucurbita maxima][more]
XP_023528894.10.096.76fumarate hydratase 1, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_004152237.10.096.15fumarate hydratase 1, mitochondrial [Cucumis sativus] >KGN52832.1 hypothetical p... [more]
KAA0044396.10.096.35fumarate hydratase 1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1DLZ00.0100.00Fumarate hydratase OS=Momordica charantia OX=3673 GN=LOC111022407 PE=3 SV=1[more]
A0A6J1J9G00.097.17Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482474 PE=3 SV=1[more]
A0A5A7TQL40.096.35Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00... [more]
A0A0A0KWM00.096.15Fumarate hydratase OS=Cucumis sativus OX=3659 GN=Csa_4G001990 PE=3 SV=1[more]
A0A5D3E1170.096.35Fumarate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0... [more]
Match NameE-valueIdentityDescription
AT2G47510.12.2e-25089.39fumarase 1 [more]
AT2G47510.22.2e-25089.39fumarase 1 [more]
AT5G50950.21.7e-24590.36FUMARASE 2 [more]
AT5G50950.11.9e-23388.79FUMARASE 2 [more]
AT5G50950.37.0e-22090.07FUMARASE 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000362Fumarate lyase familyPRINTSPR00149FUMRATELYASEcoord: 301..328
score: 38.17
coord: 347..363
score: 66.75
coord: 164..182
score: 38.81
coord: 210..228
score: 55.26
IPR005677Fumarate hydratase, class IITIGRFAMTIGR00979TIGR00979coord: 34..491
e-value: 2.6E-229
score: 759.6
IPR005677Fumarate hydratase, class IIPANTHERPTHR11444ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASEcoord: 17..494
IPR005677Fumarate hydratase, class IIHAMAPMF_00743FumaraseCcoord: 34..492
score: 88.012772
IPR005677Fumarate hydratase, class IICDDcd01362Fumarase_classIIcoord: 35..489
e-value: 0.0
score: 891.853
IPR018951Fumarase C, C-terminalPFAMPF10415FumaraseC_Ccoord: 438..491
e-value: 1.8E-24
score: 85.9
NoneNo IPR availableGENE3D1.10.40.30coord: 437..494
e-value: 1.5E-27
score: 97.6
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 170..436
e-value: 3.4E-107
score: 359.7
NoneNo IPR availablePANTHERPTHR11444:SF24FUMARATE HYDRATASE, MITOCHONDRIALcoord: 17..494
IPR022761Fumarate lyase, N-terminalPFAMPF00206Lyase_1coord: 42..372
e-value: 5.6E-117
score: 390.6
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 35..169
e-value: 2.6E-63
score: 214.2
IPR020557Fumarate lyase, conserved sitePROSITEPS00163FUMARATE_LYASEScoord: 347..356
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 34..490

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1675.1MC08g1675.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006106 fumarate metabolic process
biological_process GO:0006108 malate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005739 mitochondrion
cellular_component GO:0045239 tricarboxylic acid cycle enzyme complex
molecular_function GO:0004333 fumarate hydratase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016829 lyase activity