MC08g1658 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1658 vs. ExPASy Swiss-Prot
Match: Q0WQ75 (Vacuolar protein sorting-associated protein 51 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS51 PE=1 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 2.7e-279
Identity = 516/792 (65.15%), Postives = 632/792 (79.80%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           M  +  PMDEKAKRMRDLLSSFY+PD S+S S  GSS    +  D IN+TSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTS--GSS--INASFDNINSTSFDADQYMDL 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           +++KSNLE LLQRHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM
Sbjct: 61  MIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           +QLL+KI+ VQS+SDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY
Sbjct: 121 DQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
            DAVRFYTGAMPI K YGD+SF+DC+RASEEAI I++KNLQ KLFSDSESIQ RAEAAVL
Sbjct: 181 GDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLD PVDSLK KLLEKLEQS   LQ+  E+ +STLV+   SS +   +       HE 
Sbjct: 241 LKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEE-ASTLVEDDDSSNDTESNDQHPAKIHED 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           +VR F+EA+RAYR IF DS+ +L KL + L   HF+  E +IKK++ AAD LG+F I+W 
Sbjct: 301 AVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWE 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           +V+L+ EVL +A L D S +AAQV +KQ+V   FS L Q+ISD L +    ++E ++   
Sbjct: 361 DVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGEL 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           L++ LEAS+K VLQG+ ++  DFRQLL++++G+ I  ++LI  W+Q+G Q+FFR+L  +F
Sbjct: 421 LKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           ++LSG+ +S +      E   ++K+ AGL+LVLAQ+SVFIEQ  IPR+TE        EI
Sbjct: 481 LVLSGKTSSSND----IEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
            ASFSGG  + +E GPAF+P E+CR+F +A EK L  YI+ R+Q++S+LL KRF+TPNWV
Sbjct: 541 AASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGN-RKHRRTDSNGSTTTTSSRSNPIREE 660
           KHKEPREVHM+VD+ L E+E +G EVKQVLP+G  RKH+RTDSNGS TTTSSRSN +  +
Sbjct: 601 KHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHND 660

Query: 661 KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQT 720
           K+ RSN+QRARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+LQE+VRLQT
Sbjct: 661 KMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQT 720

Query: 721 FNRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLT 780
           FNRSGFQQIQLD+QFL+ PLKE  +DEAAIDFLLDEVIVAASERCLD +PLEPPILDKL 
Sbjct: 721 FNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLI 775

Query: 781 QAKLAKARDLNS 792
           QAKLAK+++ N+
Sbjct: 781 QAKLAKSKEHNN 775

BLAST of MC08g1658 vs. ExPASy Swiss-Prot
Match: Q54KG3 (Vacuolar protein sorting-associated protein 51 homolog OS=Dictyostelium discoideum OX=44689 GN=vps51 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.6e-61
Identity = 221/810 (27.28%), Postives = 394/810 (48.64%), Query Frame = 0

Query: 11  KAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSILVQKSNLEGL 70
           ++KR+R+LL ++Y P     GS + S N    PL+ I+  SFN + Y   +V+ S L  L
Sbjct: 147 ESKRVRNLLKTYYGPGIG-EGSDSSSMN---DPLN-IDGPSFNLNSYFDSIVKSSTLNQL 206

Query: 71  LQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILVV 130
           +Q+  +M +EI+ LD D++ LVY+NY KFI+ATD IK+M  N+  ME  M  L + + ++
Sbjct: 207 IQKDNQMVSEIRTLDGDMKTLVYDNYTKFINATDIIKKMKTNVENMEEGMALLSKNMDLI 266

Query: 131 QSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGA 190
            + S+ +N++L  +R+ I++L   +   +K+QF+  LP+ L  C+  +AY  AVR+Y   
Sbjct: 267 TNCSEKINSTLSVRRDRIDQLSGLQKFFQKLQFLTALPSSLNHCLAMQAYNQAVRYYNSN 326

Query: 191 MPIFKAYGD-SSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVD 250
             I K Y    SF++ +   +  +  +   L E+L S S S     E+A +L  L  PV+
Sbjct: 327 SGILKQYSHIPSFQNIQNECDSIMKTMKDKLYERLSSLSTSQTDCVESAEVLMDLLEPVE 386

Query: 251 SLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEASVREFAEAV 310
            ++ K LE  +  T+ L  N+E  S   +  +   KE N +           + E++  +
Sbjct: 387 LVRSKYLESRKHHTITLLENLEKKSVEHI--TDYIKELNAN----------FLSEYSYNI 446

Query: 311 RAYRVIF------ADSDR-------QLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFG 370
            +Y+ +F      +DS +       QL    +DL  K+ +  +  +       + +    
Sbjct: 447 TSYKSLFINRLDGSDSKKERQQSLLQLEDFSKDLFNKYLNIAKSKLSSFKDPIEKIMALE 506

Query: 371 IIWTNVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHS----RK 430
           II ++V  LG  L+     +         V   +   F  L + I + + Q++S    R+
Sbjct: 507 IINSDVSRLGSELSS---HEKITNIINSTVHDQIDFYFDNLQKTIKEHIHQLNSTLNERR 566

Query: 431 EEGLQEYSLQLALEASKKEVLQGSMDVLLDFRQL----LEDQSGLIINQRNLIIDWVQEG 490
           +E L+ ++LQ   +A+ K ++    D++L F  L    L  ++  + + ++ I   +Q  
Sbjct: 567 DEVLEGHNLQELSDATSKAIVN---DIILLFANLKPFFLPTETQFLSSYQDTIFTKIQVK 626

Query: 491 FQEFFRALVGRFMLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRI 550
            Q+FF  LV    L            ++   +  E+     +LVL+ I ++ E   I  +
Sbjct: 627 LQQFFLFLVNIHFL--------EYLDIIATTSNREQFSGRFLLVLSSICLYFENKGITLV 686

Query: 551 TEARHRCKIFEITASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISI 610
            +         IT +  G       +  +F   ++C+  R  G + L+++  + SQ++  
Sbjct: 687 VQLMSEF----ITVAKQGAKKELNVF--SFNAPDLCKRVRETGLQILNVFTRLSSQKLDK 746

Query: 611 LLTKRFRT---PNWVKHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGN------RKHRR 670
           +L K   +    NW+  KEPR+V    D+ L+E+    +E  ++LP  N      + H R
Sbjct: 747 ILKKGLESIPNNNWLVLKEPRDVRSVNDIYLEEILKFQNETSKLLPVINHHHHSLKSHSR 806

Query: 671 TDSNG-STTTTSSRSNPIREEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGS 730
           T S G S ++ +S SN     + N S +  + S    T    LF++K++    V+F   S
Sbjct: 807 TGSGGNSVSSNNSSSNTPDGHRRNPSASSSSSSNNTNT---TLFEKKVD---HVDFNTLS 866

Query: 731 VVTTAVKLFLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIV 789
           V+   +KL LK+  E +RL+TF  +G  QIQ+D+ +L+  L +      + D LL E   
Sbjct: 867 VLIAIIKLSLKSFNESLRLKTFGTNGCHQIQIDLHYLKLSLYDLFGSN-SFDNLLQECEN 912

BLAST of MC08g1658 vs. ExPASy Swiss-Prot
Match: Q155U0 (Vacuolar protein sorting-associated protein 51 homolog OS=Danio rerio OX=7955 GN=vps51 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.1e-57
Identity = 221/843 (26.22%), Postives = 383/843 (45.43%), Query Frame = 0

Query: 13  KRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDA--INTTSFNPDQYMSILVQKSNLEGL 72
           +R+  +L  +Y  +        G +   A  LD   IN   F+P+ Y++ L ++ +L  L
Sbjct: 16  RRVHSMLKLYYGLNEE------GKATEQAESLDPCDINGPHFDPEIYLNKLRKECSLTEL 75

Query: 73  LQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILVV 132
           +     M  +I++LD+D+Q LVYENYNKFISATDTI++M N+   ME  M+ L   +  +
Sbjct: 76  MDHESCMVKQIRSLDSDMQTLVYENYNKFISATDTIRKMKNDFKKMEDEMDCLSANMAAI 135

Query: 133 QSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGA 192
              S  ++ +L ++   I KL     LLRK+QF+++LPARL KC++ +AYA AV  +  A
Sbjct: 136 TEFSARISGTLQDQHAQITKLSGVHTLLRKLQFLFELPARLNKCLELQAYAQAVSSHRRA 195

Query: 193 MPIFKAYGD-SSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVD 252
             + + Y    SF+  +      +  + + L++K      S +  +E   LL QLD P +
Sbjct: 196 RCVLQQYSHMPSFRGIQDDCHVIMEQLAQQLRQKFRDGGSSAKDLSECVELLLQLDEPAE 255

Query: 253 SLKVKLL----EKLEQSTMDLQLNVED----------LSSTLVDRSGSSKEGNVSQSVYG 312
            L  K L     +LE     L+  ++D          +  T    +  S   +VS     
Sbjct: 256 ELCDKFLSHAQSRLEADLQGLEAELKDSAVTDTGAGSVQKTSPGSNPVSPSSSVSNPFLS 315

Query: 313 ASHEASVREFAE------------AVRAYRVIFAD---------------SDRQLIKLVQ 372
            +    + EF +             + +Y+ +F +               ++ +L   V 
Sbjct: 316 PAAGTDILEFIDRGCNEFVSNLCLVIASYQELFINRPQESELASKNIPEMANGKLHVFVD 375

Query: 373 DLITKHFDATEQFIKKQIYAAD---LLGVFGIIWTNVLLLGEVLNDAGLP----DYSMKA 432
            L  ++F   E+ I+++    D   L+         +  + ++L  + +P    +  ++A
Sbjct: 376 TLAARYFSLVERRIQEEKGVGDNSLLVRALDRFHRRLQAISKLLPGSAVPSQGTEIVVRA 435

Query: 433 AQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYSLQLALEASKKEVLQG--SMDV 492
           A+  +KQY+    S L     D+LT V  R+       S+  A  +    ++ G  S D 
Sbjct: 436 ARERIKQYL----SALQTFYHDSLTDV--RQALAAPRLSVGGASASGGGALVGGASSKDA 495

Query: 493 LLDFRQLLEDQSGLIINQRNLII---------------------DWVQEGFQEFFRALVG 552
                +LL   S  I+NQ   ++                     ++  +G +E       
Sbjct: 496 PPSLPELLTSLSNFILNQLKSVLASVHLFTAKDITFSNKPYFKGEFCSQGVREGLVVSFI 555

Query: 553 RFMLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIF 612
           +F+  S R    S G       +       L+L+L+++ +  E   I  I        + 
Sbjct: 556 KFICQSSRQYCESAGD------RGGSTPPALLLLLSRLCLDYETSTISYILTLTDEQFLV 615

Query: 613 EITASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPN 672
           +              + P    + +C   R A +K L+ Y+ ++   IS +L K   T +
Sbjct: 616 Q-------------HHTPVTPVTALCAEAREAAQKLLNHYVKVQGLIISQMLRKSVETRD 675

Query: 673 WVKHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIRE 732
           WV   EPR V   +  ++++  +I  +V  +  EG RK   +DS+  T +  S S   ++
Sbjct: 676 WVNTIEPRNVRAVMKRVVEDTTSIDVQVGLLYEEGVRKAHSSDSSKRTFSVYSSSR--QQ 735

Query: 733 EKLNRSNTQRARSQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRL 781
            +   S T  A     L +++ KLF ++I+IF+ VEF + SV+T  +K+ LKT  E VRL
Sbjct: 736 IRYAPSYTPSAPMDTNLLSNIHKLFSERIDIFSPVEFNKVSVLTGIIKISLKTFLECVRL 795

BLAST of MC08g1658 vs. ExPASy Swiss-Prot
Match: Q4V9Y0 (Vacuolar protein sorting-associated protein 51 homolog OS=Xenopus tropicalis OX=8364 GN=vps51 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 4.1e-57
Identity = 209/766 (27.28%), Postives = 366/766 (47.78%), Query Frame = 0

Query: 39  RYASPLDAINTTSFNPDQYMSILVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNK 98
           R+  P D ++   FNP+ Y++ L ++S+L  L+    +M  +I++LD+++Q LVYENYNK
Sbjct: 37  RHLEPTD-VDGVHFNPELYLTKLRKESSLSQLMDVEADMVRQIRSLDSEMQTLVYENYNK 96

Query: 99  FISATDTIKRMNNNIVGMETNMEQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLL 158
           FISATDTI++M N+   ME  M+ L   + V+   S  ++++L E+ + I KL     LL
Sbjct: 97  FISATDTIRKMKNDFKKMEDEMDGLATNMAVITEFSARISSTLQERHQQITKLSGVHTLL 156

Query: 159 RKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKAYGD-SSFKDCKRASEEAIAIVL 218
           RK+QF+++LPARL KCI+  AYA AV +++ A  +   Y    SF   +   +  +A + 
Sbjct: 157 RKLQFLFELPARLKKCIELGAYAQAVSYHSKARSVLHQYQHMPSFHGIQTDCQAIMAGLA 216

Query: 219 KNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKVKLLE----KLEQSTMDLQLNVEDL 278
             L+++    + S Q  +E   +L  L+ P   L  + L     +L     DLQ + + L
Sbjct: 217 DTLRQRFRDPASSPQDLSECVEMLLNLEEPAHLLCDEFLAHGRGRLASHLSDLQESGDIL 276

Query: 279 SSTLVDRSGSSKEGNVSQS-VYGASHEASVREFAEAVRAYRVIFADSDRQLIKLVQDLIT 338
               VDR      G +S + +  AS+++   + A +          ++ +L   +++L T
Sbjct: 277 E--FVDRGCG---GFISDACLLAASYQSLFSKEAGSTAQM------AEAKLTSFLEELST 336

Query: 339 KHFDATEQFIKKQIYAADLLGVFGIIWTNVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTC 398
            +F+  E+ ++++    D          N LL+  +       D   +  Q   K    C
Sbjct: 337 GYFELVEKRLRQEKSLGD----------NSLLVRAL-------DRFHRRLQAPSKLVPGC 396

Query: 399 TFSR-----LLQNISDALTQVHSRKEEGLQEYSLQLALEASKKEVLQGSMDVLLDFRQLL 458
            F+R     +++   + L Q     ++  Q     +    +   +    M  L D   LL
Sbjct: 397 GFNRRGTEIVVRAAQERLAQYLQALKDFFQGCLTDVRQALAAPRLPGKEMPALGD---LL 456

Query: 459 EDQSGLIINQRNLIIDWVQEGFQEFFRALVGRFMLLSGRNNSYSQGQVLTEATQAEKVVA 518
              S  ++NQ   ++  V       F A    F      +  Y +G+  ++  +   +VA
Sbjct: 457 AGLSASVLNQIKTVLAAV-----HLFTAKDVAF-----SDKPYFKGEFCSQGVREGLIVA 516

Query: 519 GLVLVLAQISVFIE------QIAIPRITEARHR-CKIFEITA-----SFSGGGIRGYEYG 578
            +  V      F E          P +     R C  +E +      + +     G ++ 
Sbjct: 517 FIKSVCQTARQFCEIPGEKGTSTPPALLLLLSRLCLDYETSTISYILTLTDEQFLGQDHS 576

Query: 579 PAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWVKHKEPREVHMFVDLL 638
           P    S +C + RS  +  L+ Y+  +   +S +L K   T +WV   EPR V   +  +
Sbjct: 577 PVTPVSSLCSLARSTAQTLLNQYVKSQGLVVSQMLRKSVETRDWVTTIEPRNVRAVMKRV 636

Query: 639 LQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEKLNRSNTQRARSQL-L 698
           ++++  +  +V  +  EG RK   +DS+  T +  S S    + +  +S T  A     L
Sbjct: 637 VEDITGVDVQVGLLYEEGVRKAHSSDSSKRTFSVYSSSR--LQGRYAQSYTPSAPMDTNL 696

Query: 699 ETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTFNRSGFQQIQLDMQF 758
            +++ KLF ++I+IF+ V+F + S++T  +K+ LKT  E VRL+TF R G QQIQ+D  +
Sbjct: 697 LSNIQKLFSERIDIFSTVQFNKVSILTGIIKISLKTFLECVRLRTFGRYGLQQIQVDCHY 756

Query: 759 LRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 781
           L+  L  F  DE  +  LLDEV+ +A+ RCLDP P+E  +++ + +
Sbjct: 757 LQLYLWRFVSDENLVHCLLDEVVGSAAHRCLDPAPMEQSVIEVICE 758

BLAST of MC08g1658 vs. ExPASy Swiss-Prot
Match: Q3UVL4 (Vacuolar protein sorting-associated protein 51 homolog OS=Mus musculus OX=10090 GN=Vps51 PE=1 SV=2)

HSP 1 Score: 222.6 bits (566), Expect = 1.6e-56
Identity = 207/787 (26.30%), Postives = 365/787 (46.38%), Query Frame = 0

Query: 10  EKAKRMRDLLSSFYS-PDASMSGSPTGSSNRYASPLDA--INTTSFNPDQYMSILVQKSN 69
           E+ ++   +L  +Y   +  ++G P G       PLD   +N   F+P+ Y+  L ++  
Sbjct: 28  ERRRKAHGMLKLYYGLSEGEVAGHPAG-----PDPLDPTDLNGAHFDPEVYLDKLRRECP 87

Query: 70  LEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEK 129
           L  L+    +M  +I+ LD+D+Q LVYENYNKFISATDTI++M N+   ME  M++L   
Sbjct: 88  LAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATN 147

Query: 130 ILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRF 189
           + V+ + S  ++ +L ++ E I KL     LLRK+QF+++LP+RL KC++  AY  AVR+
Sbjct: 148 MAVITNFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRY 207

Query: 190 YTGAMPIFKAYGD-SSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLD 249
              A  + + Y    SF+  +   +   A + + L+++          +AE   LL  L 
Sbjct: 208 QGRARAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGCSGAPEQAECVELLLALG 267

Query: 250 FPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEASVREF 309
            P + L        E+     +  +E+  S+L    G S                 V   
Sbjct: 268 EPAEEL-------CEEFLAHARGRLEEELSSLEAELGPSPPAPDVLEFTDRGGNGFVGGL 327

Query: 310 AEAVRAYRVIFADSD----RQLIKLVQDLITKHFDATEQFIKKQIYAAD---LLGVFGII 369
            +   AY+ +FA        +L    Q+L  ++F   E+ + ++   +D   L+      
Sbjct: 328 CQVAAAYQELFAAQGPAGAEKLAAFAQELGGRYFALVERRLAQEQGGSDNSLLVRALDRF 387

Query: 370 WTNVLLLGEVLNDAGLPDYSM----KAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEE 429
              +   G +L  AGL + +     + A+  +  ++    +  L +++D    + + +  
Sbjct: 388 HRRLRAPGALLAAAGLSESATEIVERVARERLSHHLQGLKAAFLSSLTDVRQALAAPRLA 447

Query: 430 GLQEYSLQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFR 489
           G +  SL   L      +L   +   L    L   +     N+     ++  +G +E   
Sbjct: 448 GKEGPSLAELLANVASSIL-SHIKTSLASVHLFTAKEVSFSNKPYFRGEFCSQGVREGLI 507

Query: 490 ALVGRFMLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHR 549
               R M  + ++   S G+      +       L+L+L+++ +  E   I  I      
Sbjct: 508 VGFIRSMCQTAQSFCDSPGE------KGGATPPALLLLLSRLCLDYETATISYILTLTDE 567

Query: 550 CKIFEITASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRF 609
             + +             +  P    S +C   R    + L  Y+ ++   IS +L K  
Sbjct: 568 QFLVQ-------------DQSPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRKSV 627

Query: 610 RTPNWVKHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSN 669
            T +W+   EPR V   +  ++++  AI  +V  +  EG RK + +DS+  T +  S S 
Sbjct: 628 ETRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSSSR 687

Query: 670 PIREEKLNRSNTQRARSQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQE 729
             ++ +   S T  A     L +++ KLF ++I++F+ VEF + SV+T  +K+ LKTL E
Sbjct: 688 --QQGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLE 747

Query: 730 FVRLQTFNRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPP 781
            VRL+TF R G QQ+Q+D  FL+  L  F  DE  +  LLDEV+ +A+ RC DPVP+EP 
Sbjct: 748 CVRLRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPS 780

BLAST of MC08g1658 vs. NCBI nr
Match: XP_022155269.1 (vacuolar protein sorting-associated protein 51 homolog [Momordica charantia])

HSP 1 Score: 1475 bits (3818), Expect = 0.0
Identity = 781/794 (98.36%), Postives = 784/794 (98.74%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGA+ + +AYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGALSVCQAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT
Sbjct: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS
Sbjct: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF
Sbjct: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITE        EI
Sbjct: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
           TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV
Sbjct: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660
           KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK
Sbjct: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660

Query: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720
           LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF
Sbjct: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720

Query: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780
           NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ
Sbjct: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780

Query: 781 AKLAKARDLNSMSP 794
           AKLAKARDLNSMSP
Sbjct: 781 AKLAKARDLNSMSP 786

BLAST of MC08g1658 vs. NCBI nr
Match: XP_038887792.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887793.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887794.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887795.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887796.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 1356 bits (3510), Expect = 0.0
Identity = 716/794 (90.18%), Postives = 749/794 (94.33%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEIDDVPMDEKAKRMRDLLSSFY+PDASMS S  GSSNRY SPL+AINTTSFNPDQYMSI
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYAPDASMSSSSMGSSNRYTSPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKIL VQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSF+DCKRASEE IA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEVIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLK KLLEKLEQST+DLQ+N EDLSS LV+  GSSK+G+ S+ VYGASHEA
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQVNAEDLSSALVN--GSSKDGSTSELVYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREFAEAVRAYRVIFADSDRQLIKL QDL+TKHFDATEQFIKKQI AADLL VFGIIWT
Sbjct: 301 SVREFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICAADLLLVFGIIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           +VLLLGEVLNDAGLPDYS+KAAQVAVKQYV C FSRLLQ+ISDALTQVH+RK+EG QEYS
Sbjct: 361 DVLLLGEVLNDAGLPDYSLKAAQVAVKQYVKCKFSRLLQDISDALTQVHTRKKEGAQEYS 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           LQLALEA+KK VLQGSMDVLLDFRQLLED+SGLIINQR+ I+DWVQEGFQ+FFRALV RF
Sbjct: 421 LQLALEATKKAVLQGSMDVLLDFRQLLEDKSGLIINQRDSIVDWVQEGFQDFFRALVDRF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           MLLSG+NNSYSQ QVLTEATQAEKV+AGLVLVLAQISVFIEQ AIPRITE        EI
Sbjct: 481 MLLSGKNNSYSQSQVLTEATQAEKVIAGLVLVLAQISVFIEQTAIPRITE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
            ASFSGGGIRGYEYGPAFVP+EICRMFR+AGEKFLHLYINMRSQRISIL TKRFRTPNWV
Sbjct: 541 AASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISILSTKRFRTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660
           KHKEPREVHMFVDL LQE+EA+GSEVKQ+LP+G RKHRRTDSNGSTT  SSRSNP+REEK
Sbjct: 601 KHKEPREVHMFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTT--SSRSNPLREEK 660

Query: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720
           LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKL LKTLQEFVR QTF
Sbjct: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTLVKLSLKTLQEFVRFQTF 720

Query: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780
           NRSGFQQIQLDMQFLRTPLKE ADDEAAIDFLLDEVIVAASERCLD +PLEPPILDKL Q
Sbjct: 721 NRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQ 780

Query: 781 AKLAKARDLNSMSP 794
           AKLAKA+D NS+SP
Sbjct: 781 AKLAKAKDQNSISP 782

BLAST of MC08g1658 vs. NCBI nr
Match: XP_008461504.1 (PREDICTED: vacuolar protein sorting-associated protein 51 homolog [Cucumis melo] >XP_008461505.1 PREDICTED: vacuolar protein sorting-associated protein 51 homolog [Cucumis melo])

HSP 1 Score: 1355 bits (3507), Expect = 0.0
Identity = 716/794 (90.18%), Postives = 750/794 (94.46%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEIDDVPMDEKAKRMRDLLSSFYSPDASMS SPTGSSNRYASPL+AINTTSFNPDQYM+I
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKIL VQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSF+DCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLKVKLLEKLEQST+DLQLN E+L+S LV+   SSK+GN S+ VYGASHEA
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVN--ASSKDGNTSELVYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREF EAVRAYRVIFADSDRQLIKL QDL+TKHFD+TEQFIKKQI AADLL VFG IWT
Sbjct: 301 SVREFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           +VLLLGEVLNDAGL DYS+KAAQVAVKQYVT TFSRLLQ+ISDALTQVH+RK+EG+QEYS
Sbjct: 361 DVLLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYS 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           LQL LEASKK VLQGSMD+LLDFRQLLEDQSGLIINQR+ I+DWVQEGFQ+FFRALV RF
Sbjct: 421 LQLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           MLLSG+NNSY+Q Q LTEATQAEKV AGLVLVLAQISVFIEQ AIPRITE        EI
Sbjct: 481 MLLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
            ASFSGGGIRGYEYGPAFVP+EICRMFR+AGEKFLHLYINMRSQRIS+LLTKRFRTPNWV
Sbjct: 541 AASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660
           KHKEPREVHMFVDL LQE+EA+GSEVKQ+LPEG RKHRRTDSNGSTT  SSRSNP+REEK
Sbjct: 601 KHKEPREVHMFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTT--SSRSNPLREEK 660

Query: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720
           LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKL LKTLQEFVRLQTF
Sbjct: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTF 720

Query: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780
           NRSGFQQIQLDMQFLRTPLKE ADDEAAIDFLLDEVIVAASERCLD +PLEPPILDKL Q
Sbjct: 721 NRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQ 780

Query: 781 AKLAKARDLNSMSP 794
           AKLAKA+D N M+P
Sbjct: 781 AKLAKAKDQNPMTP 782

BLAST of MC08g1658 vs. NCBI nr
Match: XP_004139639.1 (vacuolar protein sorting-associated protein 51 homolog [Cucumis sativus] >XP_011659140.1 vacuolar protein sorting-associated protein 51 homolog [Cucumis sativus] >KGN44805.2 hypothetical protein Csa_015985 [Cucumis sativus])

HSP 1 Score: 1353 bits (3502), Expect = 0.0
Identity = 714/794 (89.92%), Postives = 752/794 (94.71%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEI+DVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPL+AINTTSFNPDQYM+I
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKIL VQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSF+DCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLKVKLLEKLEQST+DLQLN E+L++ LV+   SSK+GN S+ VYGASHEA
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTTALVN--ASSKDGNSSELVYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREF EAVRAYRVIFADSDRQLIKL QDL+TKHFD+TEQFIKKQI AADLL VFGIIWT
Sbjct: 301 SVREFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGIIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           +VLL GEVLNDAGL DYS+KAAQVAVKQYVT TFSRLLQ+ISDALTQVH+RK+EG+QEYS
Sbjct: 361 DVLLFGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYS 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           LQL LEASKK VLQGSMDVLL+FRQLLEDQSGLIINQR+ I+DWVQEGFQ+FFRALV RF
Sbjct: 421 LQLELEASKKAVLQGSMDVLLEFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           MLLSG+NNSY+Q Q LTEATQAEKV+AGLVLVLAQISVFIEQ AIPRITE        EI
Sbjct: 481 MLLSGKNNSYTQSQALTEATQAEKVIAGLVLVLAQISVFIEQTAIPRITE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
            ASFSGGGIRGYEYGPAFVP+EICRMFR+AGEKFLHLYINMRSQRIS+LLTKRFRTPNWV
Sbjct: 541 AASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660
           K+KEPREVHMFVDL LQE+EA+GSEVKQ+LPEG RKHRRTDSNGSTT  SSRSNP+REEK
Sbjct: 601 KYKEPREVHMFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTT--SSRSNPLREEK 660

Query: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720
           LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKL LKTLQEFVRLQTF
Sbjct: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTF 720

Query: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780
           NRSGFQQIQLDMQFLRTPLKE ADDEAAIDFLLDEVIVAASERCLD +PLEPPILDKL Q
Sbjct: 721 NRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQ 780

Query: 781 AKLAKARDLNSMSP 794
           AKLAKA+D N M+P
Sbjct: 781 AKLAKAKDQNPMTP 782

BLAST of MC08g1658 vs. NCBI nr
Match: KAG6577400.1 (Vacuolar protein sorting-associated protein 51-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1346 bits (3483), Expect = 0.0
Identity = 714/795 (89.81%), Postives = 747/795 (93.96%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEID+ PMDEK KRMRDLLSSFYSPDAS SGS  GSSNRYASPL+AINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKIL VQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSF+DCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLK KLLEKLEQST+DLQLN EDLSS LV   GSSK+GNVS+SVYGASHEA
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVI--GSSKDGNVSESVYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREFAEA+RAYRVIFADSDRQLIKL QDL+TKHFDA EQFI+KQI AADLL VFGIIWT
Sbjct: 301 SVREFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRK-EEGLQEY 420
           +VLLLGEVLNDAGLPDYS+KAAQVAVKQYVTCTFSRLLQ+ISDAL QVH+RK EEG+QEY
Sbjct: 361 DVLLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEY 420

Query: 421 SLQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGR 480
           SLQLALEASKK VLQGSMDVLLDFRQLLEDQSGL I+QR+ I+DWVQEGFQ+FFRALV R
Sbjct: 421 SLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDR 480

Query: 481 FMLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFE 540
           FMLLSG+N+S+SQ QVL EATQAEKVVAGLVLVLAQ+SVFIEQ AIPRITE        E
Sbjct: 481 FMLLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITE--------E 540

Query: 541 ITASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNW 600
           I ASFSGGG RGYEYGPAFVP+EICRMFR+AGEKFLHLYINMRSQRIS+LLTKRFRTPNW
Sbjct: 541 IAASFSGGGTRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNW 600

Query: 601 VKHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREE 660
           VKHKEPREVHMFVDL LQE+EAIGSEVKQ+LP+G RKHRRTDSNGSTT  SSRSNP+REE
Sbjct: 601 VKHKEPREVHMFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTT--SSRSNPLREE 660

Query: 661 KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQT 720
           KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTL EFVRLQT
Sbjct: 661 KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQT 720

Query: 721 FNRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLT 780
           FNRSGFQQ+QLDMQFLRTPLKE ADDEAAIDFLLDEVIVAASERCLD +PLEPPILDKL 
Sbjct: 721 FNRSGFQQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLI 780

Query: 781 QAKLAKARDLNSMSP 794
           QAKLAKA+D N  SP
Sbjct: 781 QAKLAKAKDQNPTSP 783

BLAST of MC08g1658 vs. ExPASy TrEMBL
Match: A0A6J1DPR9 (Vacuolar protein sorting-associated protein 51 homolog OS=Momordica charantia OX=3673 GN=LOC111022412 PE=3 SV=1)

HSP 1 Score: 1475 bits (3818), Expect = 0.0
Identity = 781/794 (98.36%), Postives = 784/794 (98.74%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGA+ + +AYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGALSVCQAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT
Sbjct: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS
Sbjct: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF
Sbjct: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITE        EI
Sbjct: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
           TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV
Sbjct: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660
           KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK
Sbjct: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660

Query: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720
           LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF
Sbjct: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720

Query: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780
           NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ
Sbjct: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780

Query: 781 AKLAKARDLNSMSP 794
           AKLAKARDLNSMSP
Sbjct: 781 AKLAKARDLNSMSP 786

BLAST of MC08g1658 vs. ExPASy TrEMBL
Match: A0A1S3CES5 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucumis melo OX=3656 GN=LOC103500085 PE=3 SV=1)

HSP 1 Score: 1355 bits (3507), Expect = 0.0
Identity = 716/794 (90.18%), Postives = 750/794 (94.46%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEIDDVPMDEKAKRMRDLLSSFYSPDASMS SPTGSSNRYASPL+AINTTSFNPDQYM+I
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKIL VQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSF+DCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLKVKLLEKLEQST+DLQLN E+L+S LV+   SSK+GN S+ VYGASHEA
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVN--ASSKDGNTSELVYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREF EAVRAYRVIFADSDRQLIKL QDL+TKHFD+TEQFIKKQI AADLL VFG IWT
Sbjct: 301 SVREFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           +VLLLGEVLNDAGL DYS+KAAQVAVKQYVT TFSRLLQ+ISDALTQVH+RK+EG+QEYS
Sbjct: 361 DVLLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYS 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           LQL LEASKK VLQGSMD+LLDFRQLLEDQSGLIINQR+ I+DWVQEGFQ+FFRALV RF
Sbjct: 421 LQLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           MLLSG+NNSY+Q Q LTEATQAEKV AGLVLVLAQISVFIEQ AIPRITE        EI
Sbjct: 481 MLLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
            ASFSGGGIRGYEYGPAFVP+EICRMFR+AGEKFLHLYINMRSQRIS+LLTKRFRTPNWV
Sbjct: 541 AASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660
           KHKEPREVHMFVDL LQE+EA+GSEVKQ+LPEG RKHRRTDSNGSTT  SSRSNP+REEK
Sbjct: 601 KHKEPREVHMFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTT--SSRSNPLREEK 660

Query: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720
           LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKL LKTLQEFVRLQTF
Sbjct: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTF 720

Query: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780
           NRSGFQQIQLDMQFLRTPLKE ADDEAAIDFLLDEVIVAASERCLD +PLEPPILDKL Q
Sbjct: 721 NRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQ 780

Query: 781 AKLAKARDLNSMSP 794
           AKLAKA+D N M+P
Sbjct: 781 AKLAKAKDQNPMTP 782

BLAST of MC08g1658 vs. ExPASy TrEMBL
Match: A0A6J1EMM4 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111435972 PE=3 SV=1)

HSP 1 Score: 1345 bits (3480), Expect = 0.0
Identity = 713/795 (89.69%), Postives = 747/795 (93.96%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEID+ PMDEK KRMRDLLSSFYSPDAS SGS  GSSNRYASPL+AINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKIL VQSRSDGVNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSF+DCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLK KLLEKLEQST+DLQLN EDLSS LV   GSSK+GNVS+SVYGASHEA
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVI--GSSKDGNVSESVYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREFAEA+RAYRVIFADSDRQLIKL QDL+TKHFDATEQFI+KQI AADLL VFGIIWT
Sbjct: 301 SVREFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRK-EEGLQEY 420
           +VLLLGEVLNDAGLPDYS+KAAQVAVKQYVTCTFSRLLQ+ISDAL QVH+RK EEG+QEY
Sbjct: 361 DVLLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEY 420

Query: 421 SLQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGR 480
           SLQLALEASKK VLQGSMDVLLDFRQLLEDQSGL I+QR+ I+DWVQEGFQ+FFRALV R
Sbjct: 421 SLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDR 480

Query: 481 FMLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFE 540
           FMLLSG+N+S+SQ QVL EATQAEKVVAGLVLVLAQ+SVFIEQ AIPRITE        E
Sbjct: 481 FMLLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITE--------E 540

Query: 541 ITASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNW 600
           I ASFSGGG RGYEYGPAFVP+EICRMFR+AGEKFLHLYINMRSQRIS+LLTKRFRTPNW
Sbjct: 541 IAASFSGGGTRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNW 600

Query: 601 VKHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREE 660
           VKHKEPREVHMFVDL LQE+EA+GSEVKQ+LP+G RKHRRTDSNGSTT  SSRSNP+REE
Sbjct: 601 VKHKEPREVHMFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTT--SSRSNPLREE 660

Query: 661 KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQT 720
           KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTL EFVRLQT
Sbjct: 661 KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQT 720

Query: 721 FNRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLT 780
           FNRSGFQQ+QLDMQFLRTPLKE ADDEAAIDFLLDEVIVAASERCLD +PLEPPILDKL 
Sbjct: 721 FNRSGFQQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLI 780

Query: 781 QAKLAKARDLNSMSP 794
           QAKLAKA+D N  SP
Sbjct: 781 QAKLAKAKDQNPTSP 783

BLAST of MC08g1658 vs. ExPASy TrEMBL
Match: A0A6J1JAN2 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483261 PE=3 SV=1)

HSP 1 Score: 1340 bits (3469), Expect = 0.0
Identity = 712/795 (89.56%), Postives = 745/795 (93.71%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEID+  MDEK KRMRDLLSSFYSPDAS SGS  GSSNRYASPL+AINTTSFNPDQYMSI
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKIL VQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSF+DCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLK KLLEKLEQST+DLQLN EDLSS LV   GSSK+GNVS+SVYGASHE 
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVI--GSSKDGNVSESVYGASHEG 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREFAEA+RAYRVIFADSDRQLIKL QDL+TKHF+A EQFIKKQI AADLL VFGIIWT
Sbjct: 301 SVREFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFNAIEQFIKKQICAADLLRVFGIIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRK-EEGLQEY 420
           +VLLLGEVLNDAGLPDYS+KAAQVAVKQYVTC FSRLLQ+ISDAL QVH+RK EEG+QEY
Sbjct: 361 DVLLLGEVLNDAGLPDYSLKAAQVAVKQYVTCMFSRLLQDISDALAQVHTRKKEEGVQEY 420

Query: 421 SLQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGR 480
           SLQLALEASKK VLQGSMDVLLDFRQLLEDQSGL I+QR+ I+DWVQEGFQ+FFRALV R
Sbjct: 421 SLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDR 480

Query: 481 FMLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFE 540
           FMLLSG+N+SYSQ QVL EATQAEKVVAGLVLVLAQISVFIEQ AIPRITE        E
Sbjct: 481 FMLLSGKNSSYSQSQVLPEATQAEKVVAGLVLVLAQISVFIEQTAIPRITE--------E 540

Query: 541 ITASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNW 600
           I ASFSGGG RGYEYGPAFVP+EICRMFR+AGEKFLHLYINMRSQRIS+LLTKRFRTPNW
Sbjct: 541 IAASFSGGGTRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNW 600

Query: 601 VKHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREE 660
           VKHKEPREVHMFVDL LQE+EAIGSEVKQ+LP+G RKHRRTDSNGSTT  SSRSNP+REE
Sbjct: 601 VKHKEPREVHMFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTT--SSRSNPLREE 660

Query: 661 KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQT 720
           KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTL EF+RLQT
Sbjct: 661 KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFIRLQT 720

Query: 721 FNRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLT 780
           FNRSGFQQ+QLDMQFLRTPLKE ADDEAAIDFLLDEVIVAASERCLD +PLEPPILDKL 
Sbjct: 721 FNRSGFQQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLI 780

Query: 781 QAKLAKARDLNSMSP 794
           +AKLAKA+D N MSP
Sbjct: 781 KAKLAKAKDQNPMSP 783

BLAST of MC08g1658 vs. ExPASy TrEMBL
Match: A0A6J1K9H7 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111492889 PE=3 SV=1)

HSP 1 Score: 1329 bits (3440), Expect = 0.0
Identity = 704/794 (88.66%), Postives = 743/794 (93.58%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           MEI+DVPMDEKAKRMRDLLSSFYSPDASMSGS TGSSNRYASPL+ INTTSFNPDQYMSI
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTGSSNRYASPLETINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQK+NLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKANLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKIL VQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSF+DCKRASEEAIAIVLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLDFPVDSLK KLLEKLEQST+DLQLN +DLSS L+    S KE N S+S YGASHEA
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNADDLSSGLLI--ASPKERNDSESAYGASHEA 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           SVREFAEAVRAYRVIFADSDRQLIKL QDL+TKHFDA EQFIKKQI+AADLL VFGIIWT
Sbjct: 301 SVREFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWT 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           +VLLLGEVLNDA L DYS+KAAQVAVKQYVTCTFSRLLQNISDALT+VH+RK+E +QEYS
Sbjct: 361 DVLLLGEVLNDACLHDYSLKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYS 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           LQLALEA KK VLQGSMDVLLDFR LLE+QSGLIINQR+ I+DWVQEGFQ+FFR LV RF
Sbjct: 421 LQLALEAGKKAVLQGSMDVLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRELVDRF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           MLLSG+NNSYSQ QVLTEA QA+KVVAGLVLVLAQIS+FIEQ AI RITE        EI
Sbjct: 481 MLLSGKNNSYSQSQVLTEANQADKVVAGLVLVLAQISIFIEQTAIHRITE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
            ASFSGGG+RGYEYGPAFVP+EICRMFR+AGEKFLHLYINMRSQRIS+LLTKRFRTPNWV
Sbjct: 541 AASFSGGGVRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGNRKHRRTDSNGSTTTTSSRSNPIREEK 660
           KHKEPREVHMFVDL LQE+EA+GSEV+Q+L +G RKHRRT+SNGSTT  SSRS P+REEK
Sbjct: 601 KHKEPREVHMFVDLFLQELEAVGSEVRQILLQGTRKHRRTNSNGSTT--SSRSGPLREEK 660

Query: 661 LNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQTF 720
           LNRS+ QRARSQLLE+HLAKLFKQKIEIFTRVEFTQGSVVTTAVKL LKTLQEFVRLQT+
Sbjct: 661 LNRSSAQRARSQLLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTY 720

Query: 721 NRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLTQ 780
           NRSGFQQIQLDMQFLRTPLKE ADDEAAIDFLLDEVIVAASERCLDP+PLEPPILDKL Q
Sbjct: 721 NRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQ 780

Query: 781 AKLAKARDLNSMSP 794
           AKLAKARD NS+SP
Sbjct: 781 AKLAKARDQNSISP 782

BLAST of MC08g1658 vs. TAIR 10
Match: AT4G02030.1 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 962.6 bits (2487), Expect = 1.9e-280
Identity = 516/792 (65.15%), Postives = 632/792 (79.80%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           M  +  PMDEKAKRMRDLLSSFY+PD S+S S  GSS    +  D IN+TSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTS--GSS--INASFDNINSTSFDADQYMDL 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           +++KSNLE LLQRHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM
Sbjct: 61  MIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNM 120

Query: 121 EQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           +QLL+KI+ VQS+SDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY
Sbjct: 121 DQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
            DAVRFYTGAMPI K YGD+SF+DC+RASEEAI I++KNLQ KLFSDSESIQ RAEAAVL
Sbjct: 181 GDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSSTLVDRSGSSKEGNVSQSVYGASHEA 300
           LKQLD PVDSLK KLLEKLEQS   LQ+  E+ +STLV+   SS +   +       HE 
Sbjct: 241 LKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEE-ASTLVEDDDSSNDTESNDQHPAKIHED 300

Query: 301 SVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHFDATEQFIKKQIYAADLLGVFGIIWT 360
           +VR F+EA+RAYR IF DS+ +L KL + L   HF+  E +IKK++ AAD LG+F I+W 
Sbjct: 301 AVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWE 360

Query: 361 NVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFSRLLQNISDALTQVHSRKEEGLQEYS 420
           +V+L+ EVL +A L D S +AAQV +KQ+V   FS L Q+ISD L +    ++E ++   
Sbjct: 361 DVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGEL 420

Query: 421 LQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLIINQRNLIIDWVQEGFQEFFRALVGRF 480
           L++ LEAS+K VLQG+ ++  DFRQLL++++G+ I  ++LI  W+Q+G Q+FFR+L  +F
Sbjct: 421 LKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQF 480

Query: 481 MLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQISVFIEQIAIPRITEARHRCKIFEI 540
           ++LSG+ +S +      E   ++K+ AGL+LVLAQ+SVFIEQ  IPR+TE        EI
Sbjct: 481 LVLSGKTSSSND----IEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTE--------EI 540

Query: 541 TASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFLHLYINMRSQRISILLTKRFRTPNWV 600
            ASFSGG  + +E GPAF+P E+CR+F +A EK L  YI+ R+Q++S+LL KRF+TPNWV
Sbjct: 541 AASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWV 600

Query: 601 KHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGN-RKHRRTDSNGSTTTTSSRSNPIREE 660
           KHKEPREVHM+VD+ L E+E +G EVKQVLP+G  RKH+RTDSNGS TTTSSRSN +  +
Sbjct: 601 KHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHND 660

Query: 661 KLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLFLKTLQEFVRLQT 720
           K+ RSN+QRARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+LQE+VRLQT
Sbjct: 661 KMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQT 720

Query: 721 FNRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLDEVIVAASERCLDPVPLEPPILDKLT 780
           FNRSGFQQIQLD+QFL+ PLKE  +DEAAIDFLLDEVIVAASERCLD +PLEPPILDKL 
Sbjct: 721 FNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLI 775

Query: 781 QAKLAKARDLNS 792
           QAKLAK+++ N+
Sbjct: 781 QAKLAKSKEHNN 775

BLAST of MC08g1658 vs. TAIR 10
Match: AT4G02030.2 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 948.7 bits (2451), Expect = 2.9e-276
Identity = 516/817 (63.16%), Postives = 632/817 (77.36%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLDAINTTSFNPDQYMSI 60
           M  +  PMDEKAKRMRDLLSSFY+PD S+S S  GSS    +  D IN+TSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTS--GSS--INASFDNINSTSFDADQYMDL 60

Query: 61  L-------------------------VQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYEN 120
           +                         ++KSNLE LLQRHV+MAAEIKNLDTDLQMLVYEN
Sbjct: 61  MIRLFDLGLSDLPAMVDSSELNWVYEIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYEN 120

Query: 121 YNKFISATDTIKRMNNNIVGMETNMEQLLEKILVVQSRSDGVNTSLFEKREHIEKLHRTR 180
           YNKFISATDTIKRM +NI GME NM+QLL+KI+ VQS+SDGVNTSLFEKREHIEKLHRTR
Sbjct: 121 YNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTR 180

Query: 181 NLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKAYGDSSFKDCKRASEEAIAI 240
           NLLRKVQFIYDLPARL KCIK+EAY DAVRFYTGAMPI K YGD+SF+DC+RASEEAI I
Sbjct: 181 NLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEI 240

Query: 241 VLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKVKLLEKLEQSTMDLQLNVEDLSS 300
           ++KNLQ KLFSDSESIQ RAEAAVLLKQLD PVDSLK KLLEKLEQS   LQ+  E+ +S
Sbjct: 241 IIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEE-AS 300

Query: 301 TLVDRSGSSKEGNVSQSVYGASHEASVREFAEAVRAYRVIFADSDRQLIKLVQDLITKHF 360
           TLV+   SS +   +       HE +VR F+EA+RAYR IF DS+ +L KL + L   HF
Sbjct: 301 TLVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFPDSEERLFKLARALTAMHF 360

Query: 361 DATEQFIKKQIYAADLLGVFGIIWTNVLLLGEVLNDAGLPDYSMKAAQVAVKQYVTCTFS 420
           +  E +IKK++ AAD LG+F I+W +V+L+ EVL +A L D S +AAQV +KQ+V   FS
Sbjct: 361 EYMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFS 420

Query: 421 RLLQNISDALTQVHSRKEEGLQEYSLQLALEASKKEVLQGSMDVLLDFRQLLEDQSGLII 480
            L Q+ISD L +    ++E ++   L++ LEAS+K VLQG+ ++  DFRQLL++++G+ I
Sbjct: 421 HLQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFI 480

Query: 481 NQRNLIIDWVQEGFQEFFRALVGRFMLLSGRNNSYSQGQVLTEATQAEKVVAGLVLVLAQ 540
             ++LI  W+Q+G Q+FFR+L  +F++LSG+ +S +      E   ++K+ AGL+LVLAQ
Sbjct: 481 KMKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSND----IEGKSSDKIHAGLILVLAQ 540

Query: 541 ISVFIEQIAIPRITEARHRCKIFEITASFSGGGIRGYEYGPAFVPSEICRMFRSAGEKFL 600
           +SVFIEQ  IPR+TE        EI ASFSGG  + +E GPAF+P E+CR+F +A EK L
Sbjct: 541 LSVFIEQKVIPRVTE--------EIAASFSGGNSQAFENGPAFIPGELCRVFHAASEKLL 600

Query: 601 HLYINMRSQRISILLTKRFRTPNWVKHKEPREVHMFVDLLLQEMEAIGSEVKQVLPEGN- 660
             YI+ R+Q++S+LL KRF+TPNWVKHKEPREVHM+VD+ L E+E +G EVKQVLP+G  
Sbjct: 601 QHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTF 660

Query: 661 RKHRRTDSNGSTTTTSSRSNPIREEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEF 720
           RKH+RTDSNGS TTTSSRSN +  +K+ RSN+QRARSQL ETHLAKLFKQK+EIFT+VEF
Sbjct: 661 RKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEF 720

Query: 721 TQGSVVTTAVKLFLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEFADDEAAIDFLLD 780
           TQ SVVTT VKL LK+LQE+VRLQTFNRSGFQQIQLD+QFL+ PLKE  +DEAAIDFLLD
Sbjct: 721 TQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLD 780

Query: 781 EVIVAASERCLDPVPLEPPILDKLTQAKLAKARDLNS 792
           EVIVAASERCLD +PLEPPILDKL QAKLAK+++ N+
Sbjct: 781 EVIVAASERCLDVIPLEPPILDKLIQAKLAKSKEHNN 800