MC08g1511 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1511
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionmetalloendoproteinase 2-MMP
LocationMC08: 20872059 .. 20873168 (-)
RNA-Seq ExpressionMC08g1511
SyntenyMC08g1511
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCATTGTCCTCCTCCTTATTTCCCTCTCCCTCTTCTCCCCTCCAATAATCAACGCCCACTTGTTCCCCAACGCCTCCGCCATCCCCTCGTGGCTCAACAACACCAAATGCCTTTGGGACCAATTCCAGAAGCTCAAAGGATGCCGCCCCGGCGACCGCCTCCAAGGCCTCTCCTCTCTCAAGACTTACTTCAAACACTTCGGCTACATCCCTAATCCCAATCCCAACTCCAATTTCACCGATCTCTTCGACGATATTCTCCACTCCGCCCTCACCACCTACCAGAAGAATTTCAATCTCAACGTCTCCGGCGAGCTCGATGACGACACTCTCCGCCAGATCCAGCTCCCCAGATGCGGCGTCGCCGATATAATCAATGGCTCCAGTTCTATGAACTCCGGGAACCCCGCTGCCCATGCCGCTTCCGGTTCTGCTCGCTTCCACTCTGTTTCTCACTACTCTTTCTTCCCAGGGAAGCCGCGCTGGCCGGCGGGTCGCCGGGATCTGACTTATGCCTTCGCGCCGGAGAATCAGCTCAGCGCTGAGGTGAAGGCTGTGTTCGGCCGGGCGTTCGGGCGGTGGGCGGCGGTGACGCCGTTGACTTTCACGGAGGTTCAGTCGTTCAGGGCGGCGGATATTAGGATTGGGTTCTTCGCTGGAGATCACGGCGATGGGGAGCCGTTCGACGGCGTTTTGGGGACTCTGGCGCACGCGTTTTCGCCGCCGAGTGGGCACTTCCATCTGGACGGCGACGAGAGCTGGGTGGTCTCCGGGGACCTGGGGACGGCTCCGGCGGCGGCGATTGACCTGGAGTCGGTGGCGGTGCACGAGATTGGGCATTTGTTGGGGCTGGGCCACTCCTCCGTCGAGGAATCGATTATGTTCCCGACGATCACGTCGCGGACGAGGAAGGTGGATCTCGCCGCCGACGACGTTGCCGGAGTGCAGGAGCTATACGGCGGCAATCCTAACGGCGACAACTCGACGACGACGACGATGCCGCCGTCGTCCGTACAGGAGAGGGACACGTCCAAAAACGGGGCTGCCACGCTGCTCAGCCTCTGGTGGGGCCCACTTTTACTTACTGCCGTTGAACTTTTCCTGATGAGA

mRNA sequence

TTCATTGTCCTCCTCCTTATTTCCCTCTCCCTCTTCTCCCCTCCAATAATCAACGCCCACTTGTTCCCCAACGCCTCCGCCATCCCCTCGTGGCTCAACAACACCAAATGCCTTTGGGACCAATTCCAGAAGCTCAAAGGATGCCGCCCCGGCGACCGCCTCCAAGGCCTCTCCTCTCTCAAGACTTACTTCAAACACTTCGGCTACATCCCTAATCCCAATCCCAACTCCAATTTCACCGATCTCTTCGACGATATTCTCCACTCCGCCCTCACCACCTACCAGAAGAATTTCAATCTCAACGTCTCCGGCGAGCTCGATGACGACACTCTCCGCCAGATCCAGCTCCCCAGATGCGGCGTCGCCGATATAATCAATGGCTCCAGTTCTATGAACTCCGGGAACCCCGCTGCCCATGCCGCTTCCGGTTCTGCTCGCTTCCACTCTGTTTCTCACTACTCTTTCTTCCCAGGGAAGCCGCGCTGGCCGGCGGGTCGCCGGGATCTGACTTATGCCTTCGCGCCGGAGAATCAGCTCAGCGCTGAGGTGAAGGCTGTGTTCGGCCGGGCGTTCGGGCGGTGGGCGGCGGTGACGCCGTTGACTTTCACGGAGGTTCAGTCGTTCAGGGCGGCGGATATTAGGATTGGGTTCTTCGCTGGAGATCACGGCGATGGGGAGCCGTTCGACGGCGTTTTGGGGACTCTGGCGCACGCGTTTTCGCCGCCGAGTGGGCACTTCCATCTGGACGGCGACGAGAGCTGGGTGGTCTCCGGGGACCTGGGGACGGCTCCGGCGGCGGCGATTGACCTGGAGTCGGTGGCGGTGCACGAGATTGGGCATTTGTTGGGGCTGGGCCACTCCTCCGTCGAGGAATCGATTATGTTCCCGACGATCACGTCGCGGACGAGGAAGGTGGATCTCGCCGCCGACGACGTTGCCGGAGTGCAGGAGCTATACGGCGGCAATCCTAACGGCGACAACTCGACGACGACGACGATGCCGCCGTCGTCCGTACAGGAGAGGGACACGTCCAAAAACGGGGCTGCCACGCTGCTCAGCCTCTGGTGGGGCCCACTTTTACTTACTGCCGTTGAACTTTTCCTGATGAGA

Coding sequence (CDS)

TTCATTGTCCTCCTCCTTATTTCCCTCTCCCTCTTCTCCCCTCCAATAATCAACGCCCACTTGTTCCCCAACGCCTCCGCCATCCCCTCGTGGCTCAACAACACCAAATGCCTTTGGGACCAATTCCAGAAGCTCAAAGGATGCCGCCCCGGCGACCGCCTCCAAGGCCTCTCCTCTCTCAAGACTTACTTCAAACACTTCGGCTACATCCCTAATCCCAATCCCAACTCCAATTTCACCGATCTCTTCGACGATATTCTCCACTCCGCCCTCACCACCTACCAGAAGAATTTCAATCTCAACGTCTCCGGCGAGCTCGATGACGACACTCTCCGCCAGATCCAGCTCCCCAGATGCGGCGTCGCCGATATAATCAATGGCTCCAGTTCTATGAACTCCGGGAACCCCGCTGCCCATGCCGCTTCCGGTTCTGCTCGCTTCCACTCTGTTTCTCACTACTCTTTCTTCCCAGGGAAGCCGCGCTGGCCGGCGGGTCGCCGGGATCTGACTTATGCCTTCGCGCCGGAGAATCAGCTCAGCGCTGAGGTGAAGGCTGTGTTCGGCCGGGCGTTCGGGCGGTGGGCGGCGGTGACGCCGTTGACTTTCACGGAGGTTCAGTCGTTCAGGGCGGCGGATATTAGGATTGGGTTCTTCGCTGGAGATCACGGCGATGGGGAGCCGTTCGACGGCGTTTTGGGGACTCTGGCGCACGCGTTTTCGCCGCCGAGTGGGCACTTCCATCTGGACGGCGACGAGAGCTGGGTGGTCTCCGGGGACCTGGGGACGGCTCCGGCGGCGGCGATTGACCTGGAGTCGGTGGCGGTGCACGAGATTGGGCATTTGTTGGGGCTGGGCCACTCCTCCGTCGAGGAATCGATTATGTTCCCGACGATCACGTCGCGGACGAGGAAGGTGGATCTCGCCGCCGACGACGTTGCCGGAGTGCAGGAGCTATACGGCGGCAATCCTAACGGCGACAACTCGACGACGACGACGATGCCGCCGTCGTCCGTACAGGAGAGGGACACGTCCAAAAACGGGGCTGCCACGCTGCTCAGCCTCTGGTGGGGCCCACTTTTACTTACTGCCGTTGAACTTTTCCTGATGAGA

Protein sequence

FIVLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGVADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAATLLSLWWGPLLLTAVELFLMR
Homology
BLAST of MC08g1511 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 6.7e-111
Identity = 202/354 (57.06%), Postives = 251/354 (70.90%), Query Frame = 0

Query: 2   IVLLLISLSLFSPPIINAHLFPNASAIPSWL--NNTKCLWDQFQKLKGCRPGDRLQGLSS 61
           I + ++ L  F+P  ++A  + N+SAIP  L  N T   W+ F    GC  G +  GL  
Sbjct: 4   ICVFMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYDGLYM 63

Query: 62  LKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRC 121
           LK YF+HFGYI   N + NFTD FDDIL +A+  YQ+NF LNV+G LD+ TL+ + +PRC
Sbjct: 64  LKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVVIPRC 123

Query: 122 GVADIINGSSSMNSGNPAAHA--ASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPEN 181
           G  D++NG+S+M+SG        A    RFH+V HYSFFPG+PRWP  RRDLTYAF P N
Sbjct: 124 GNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFDPRN 183

Query: 182 QLSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAH 241
            L+ EVK+VF RAF RW  VTPLTFT V+ F  +DI IGF++G+HGDGEPFDG + TLAH
Sbjct: 184 ALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAH 243

Query: 242 AFSPPSGHFHLDGDESWVVSGDLG---TAPAAAIDLESVAVHEIGHLLGLGHSSVEESIM 301
           AFSPP+GHFHLDG+E+W+VSG+ G    + + A+DLESVAVHEIGHLLGLGHSSVE SIM
Sbjct: 244 AFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIM 303

Query: 302 FPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGA 349
           +PTI +  RKVDL  DDV GVQ LYG NPN + S +   PP S Q+RDT  +GA
Sbjct: 304 YPTIRTGRRKVDLTTDDVEGVQYLYGANPNFNGSRS---PPPSTQQRDTGDSGA 354

BLAST of MC08g1511 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.5e-107
Identity = 206/354 (58.19%), Postives = 248/354 (70.06%), Query Frame = 0

Query: 1   FIVLLLISLSLFSPPIINAHLFPNASAI-PSWLNNTKCLWDQFQKLKGCRPGDRLQGLSS 60
           F V   +SL L   P  +A  FPN++A+ PS  N T+  WD F    GC  G  + GL  
Sbjct: 3   FCVFGFLSLFLIVSP-ASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYR 62

Query: 61  LKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRC 120
           +K YF+ FGYIP    + NFTD FDDIL +A+  YQ NFNLNV+GELD  T++ I +PRC
Sbjct: 63  IKKYFQRFGYIPE-TFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRC 122

Query: 121 GVADIINGSSSMNSGNPAAHAASGS-ARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQ 180
           G  D++NG+S M+ G       + S    H+V  Y+ FPG+PRWP  RRDLTYAF P+N 
Sbjct: 123 GNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNP 182

Query: 181 LSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHA 240
           L+ EVK+VF RAFGRW+ VT L FT  +SF  +DI IGF+ GDHGDGEPFDGVLGTLAHA
Sbjct: 183 LTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHA 242

Query: 241 FSPPSGHFHLDGDESWVVSGDLGT--APAAAIDLESVAVHEIGHLLGLGHSSVEESIMFP 300
           FSPPSG FHLD DE+WVVSGDL +  +  AA+DLESVAVHEIGHLLGLGHSSVEESIM+P
Sbjct: 243 FSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYP 302

Query: 301 TITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQ-ERDTSKNGAA 350
           TIT+  RKVDL  DDV G+Q LYG NPN +    TT PPS+ + +RDT    AA
Sbjct: 303 TITTGKRKVDLTNDDVEGIQYLYGANPNFNG---TTSPPSTTKHQRDTGGFSAA 351

BLAST of MC08g1511 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 4.8e-101
Identity = 193/358 (53.91%), Postives = 236/358 (65.92%), Query Frame = 0

Query: 3   VLLLISLSLFSPPIINAHLFPNASAIP--SWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 62
           +LL I +  F+   I+A  + N S+IP   +LN T+  W+ F KL GC  G+ + GLS L
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKL 63

Query: 63  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 122
           K YF+ FGYI       N TD FDD+L SA+ TYQKNFNL V+G+LD  TLRQI  PRCG
Sbjct: 64  KQYFRRFGYI---TTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 123

Query: 123 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 182
             D+I+G S MN G              +   YSFFPGKPRWP  +RDLTYAFAP+N L+
Sbjct: 124 NPDLIDGVSEMNGGK----------ILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLT 183

Query: 183 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 242
            EVK VF RAF RWA VTPL FT  +S   ADI IGFF+G+HGDGEPFDG +GTLAHA S
Sbjct: 184 DEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASS 243

Query: 243 PPSGHFHLDGDESWVVS-GDLG---TAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFP 302
           PP+G  HLDGDE W++S G++          +DLESVAVHEIGHLLGLGHSSVE++IMFP
Sbjct: 244 PPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFP 303

Query: 303 TITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAATLLSL 355
            I+   RKV+LA DD+ G+Q LYGGNPNGD   +     S     D+ +     ++SL
Sbjct: 304 AISGGDRKVELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQSTGGDSVRRWRGWMISL 348

BLAST of MC08g1511 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.7e-75
Identity = 147/317 (46.37%), Postives = 195/317 (61.51%), Query Frame = 0

Query: 39  WDQFQKLKGCRPGDRLQGLSSLKTYFKHFGYIPNPNPNSN-FTDLFDDILHSALTTYQKN 98
           W  F +L   + G  + G+S LK Y   FGY+   N  S  F+D+FD  L SA++ YQ+N
Sbjct: 52  WHDFSRLVDVQIGSHVSGVSELKRYLHRFGYV---NDGSEIFSDVFDGPLESAISLYQEN 111

Query: 99  FNLNVSGELDDDTLRQIQLPRCGVADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFP 158
             L ++G LD  T+  + LPRCGV+D               H    +   H+ +HY++F 
Sbjct: 112 LGLPITGRLDTSTVTLMSLPRCGVSD--------------THMTINNDFLHTTAHYTYFN 171

Query: 159 GKPRWPAGRRDLTYAFAPENQL----SAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADI 218
           GKP+W   R  LTYA +  ++L    S +VK VF RAF +W++V P++F EV  F  AD+
Sbjct: 172 GKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADL 231

Query: 219 RIGFFAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDGDESWVVSGDLGTAPAAAIDLESV 278
           +IGF+AGDHGDG PFDGVLGTLAHAF+P +G  HLD  E+W+V  DL  +   A+DLESV
Sbjct: 232 KIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESV 291

Query: 279 AVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTM 338
           A HEIGHLLGLGHSS E ++M+P++  RT+KVDL  DDVAGV +LYG NP          
Sbjct: 292 ATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPNPK-------LR 342

Query: 339 PPSSVQERDTSKNGAAT 351
             S  Q  D+ KNG  +
Sbjct: 352 LDSLTQSEDSIKNGTVS 342

BLAST of MC08g1511 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 3.8e-74
Identity = 149/308 (48.38%), Postives = 190/308 (61.69%), Query Frame = 0

Query: 19  AHLFPNASAIPSWLNNTKCLWD--QFQKLKGCRPGDRLQGLSSLKTYFKHFGYIPNPNPN 78
           A L+  A+++PS   +    WD     K     PG   +GLS++K YF H GYIPN    
Sbjct: 14  ATLYFLATSLPSVSAHGPYAWDGEATYKFTTYHPGQNYKGLSNVKNYFHHLGYIPNA--- 73

Query: 79  SNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGVADIINGSSSMNSGNP 138
            +F D FDD L SA+ TYQKN+NLNV+G+ D +TL+QI  PRCGV DII  ++   S   
Sbjct: 74  PHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDIIINTNKTTS--- 133

Query: 139 AAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSAEVKAVFGRAFGRWAA 198
                     F  +S Y+FF   PRW AG   LTYAF+PE +L    K+   RAF +W  
Sbjct: 134 ----------FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTP 193

Query: 199 VTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDGDESWVV 258
           V  + F E  S+  A+I+I F + +HGD  PFDG  G L HAF+P  G  H D DE WV 
Sbjct: 194 VVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVA 253

Query: 259 SGDLGTAP-AAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAADDVAGV 318
           SGD+  +P  +A DLESVAVHEIGHLLGLGHSS   +IM+P+I  RTRKV+LA DD+ G+
Sbjct: 254 SGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGI 305

Query: 319 QELYGGNP 324
           ++LYG NP
Sbjct: 314 RKLYGINP 305

BLAST of MC08g1511 vs. NCBI nr
Match: XP_022999578.1 (metalloendoproteinase 2-MMP [Cucurbita maxima])

HSP 1 Score: 569 bits (1467), Expect = 8.06e-202
Identity = 287/371 (77.36%), Postives = 317/371 (85.44%), Query Frame = 0

Query: 3   VLLLISLSLFSPPI-INAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLK 62
           +L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCR GD L GLS+LK
Sbjct: 7   ILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRHGDHLTGLSNLK 66

Query: 63  TYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGV 122
           TY   FGYIPN +P SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG+
Sbjct: 67  TYLNKFGYIPN-SP-SNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGI 126

Query: 123 ADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSA 182
           ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+ 
Sbjct: 127 ADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTD 186

Query: 183 EVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSP 242
           EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFSP
Sbjct: 187 EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSP 246

Query: 243 PSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 302
           PSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR
Sbjct: 247 PSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 306

Query: 303 TRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTSKNGAATLLSLWWGPL 362
           TRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD SK GA  +L+ WW PL
Sbjct: 307 TRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSKGGAPKVLNSWWSPL 366

Query: 363 LLTAVELFLMR 370
           ++ AVELFLMR
Sbjct: 367 VVAAVELFLMR 372

BLAST of MC08g1511 vs. NCBI nr
Match: XP_022946065.1 (metalloendoproteinase 5-MMP-like [Cucurbita moschata])

HSP 1 Score: 568 bits (1465), Expect = 1.69e-201
Identity = 288/372 (77.42%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 3   VLLLISLSLFSPPI-INAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLK 62
           +L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCRPGD L GLS+LK
Sbjct: 7   ILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLNGLSNLK 66

Query: 63  TYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGV 122
           TY   FGYIPN +P SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG+
Sbjct: 67  TYLNKFGYIPN-SP-SNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGI 126

Query: 123 ADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSA 182
           ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+ 
Sbjct: 127 ADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTD 186

Query: 183 EVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSP 242
           EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFSP
Sbjct: 187 EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSP 246

Query: 243 PSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 302
           PSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR
Sbjct: 247 PSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 306

Query: 303 TRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTS-KNGAATLLSLWWGP 362
           TRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD S K GA  +L+ WW P
Sbjct: 307 TRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSP 366

Query: 363 LLLTAVELFLMR 370
           L++ AVELFLMR
Sbjct: 367 LVVAAVELFLMR 373

BLAST of MC08g1511 vs. NCBI nr
Match: KAG7029995.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568 bits (1464), Expect = 2.39e-201
Identity = 288/372 (77.42%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 3   VLLLISLSLFSPPI-INAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLK 62
           +L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCRPGD L GLS+LK
Sbjct: 7   ILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLK 66

Query: 63  TYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGV 122
           TY   FGYIPN +P SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG+
Sbjct: 67  TYLNKFGYIPN-SP-SNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGI 126

Query: 123 ADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSA 182
           ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+ 
Sbjct: 127 ADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTD 186

Query: 183 EVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSP 242
           EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFSP
Sbjct: 187 EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSP 246

Query: 243 PSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 302
           PSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR
Sbjct: 247 PSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 306

Query: 303 TRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTS-KNGAATLLSLWWGP 362
           TRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD S K GA  +L+ WW P
Sbjct: 307 TRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSP 366

Query: 363 LLLTAVELFLMR 370
           L++ AVELFLMR
Sbjct: 367 LVVAAVELFLMR 373

BLAST of MC08g1511 vs. NCBI nr
Match: XP_023546725.1 (metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566 bits (1458), Expect = 1.96e-200
Identity = 287/372 (77.15%), Postives = 317/372 (85.22%), Query Frame = 0

Query: 3   VLLLISLSLFSPPI-INAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLK 62
           +L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCRPGD L GLS+LK
Sbjct: 7   ILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLK 66

Query: 63  TYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGV 122
           TY   FGYIPN +P SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG+
Sbjct: 67  TYLNKFGYIPN-SP-SNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGI 126

Query: 123 ADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSA 182
           ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+ 
Sbjct: 127 ADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTN 186

Query: 183 EVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSP 242
           EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFSP
Sbjct: 187 EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSP 246

Query: 243 PSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 302
           PSGHFHLD DESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR
Sbjct: 247 PSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 306

Query: 303 TRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTS-KNGAATLLSLWWGP 362
           TRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD S K GA  +L+ WW P
Sbjct: 307 TRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSP 366

Query: 363 LLLTAVELFLMR 370
           L++ AVELFLMR
Sbjct: 367 LVVAAVELFLMR 373

BLAST of MC08g1511 vs. NCBI nr
Match: XP_038889498.1 (metalloendoproteinase 2-MMP [Benincasa hispida])

HSP 1 Score: 563 bits (1452), Expect = 1.16e-199
Identity = 284/369 (76.96%), Postives = 311/369 (84.28%), Query Frame = 0

Query: 5   LLISLSLF--SPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLKT 64
           LLISLSL   + PI +AH+FPN S+IPSWLN T C W+QFQKLKGCRPGDRL GLS+LKT
Sbjct: 4   LLISLSLLLLAYPI-SAHIFPNVSSIPSWLNKTNCAWNQFQKLKGCRPGDRLNGLSNLKT 63

Query: 65  YFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGVA 124
           Y K FGYIP   P+ NFTDLFD +L SAL TYQ NFNLNV+G LDD TL QIQLPRCG+ 
Sbjct: 64  YLKQFGYIP---PSPNFTDLFDPLLQSALQTYQTNFNLNVTGLLDDQTLAQIQLPRCGIP 123

Query: 125 DIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSAE 184
           DIIN SSSMNSG P  H+A+    FHSVSHYSFFPG+P WP+ RR LTYAFAPEN LS E
Sbjct: 124 DIINNSSSMNSGKPTGHSAT---HFHSVSHYSFFPGRPTWPSHRRHLTYAFAPENPLSDE 183

Query: 185 VKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSPP 244
           VK VF RAF RWAAVTPLTF+ V+SFR ADIRIGF+AGDHGDGEPFDGVLGTLAHAFSPP
Sbjct: 184 VKGVFARAFARWAAVTPLTFSPVESFRTADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPP 243

Query: 245 SGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRT 304
           SGHFHLDGDESWVVSGDL +AP AAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRT
Sbjct: 244 SGHFHLDGDESWVVSGDLRSAPMAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRT 303

Query: 305 RKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAAT-LLSLWWGPLLL 364
           RKVDLAADD+ G+QELYGGNP+G+ +  +T  P SVQERD S+NGAA  LLS WW PLL+
Sbjct: 304 RKVDLAADDINGIQELYGGNPDGNLTPPSTPSPPSVQERDMSRNGAAPRLLSFWWSPLLI 363

Query: 365 TAVELFLMR 370
            AVE FL+R
Sbjct: 364 AAVEFFLIR 365

BLAST of MC08g1511 vs. ExPASy TrEMBL
Match: A0A6J1KK37 (metalloendoproteinase 2-MMP OS=Cucurbita maxima OX=3661 GN=LOC111493905 PE=3 SV=1)

HSP 1 Score: 569 bits (1467), Expect = 3.90e-202
Identity = 287/371 (77.36%), Postives = 317/371 (85.44%), Query Frame = 0

Query: 3   VLLLISLSLFSPPI-INAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLK 62
           +L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCR GD L GLS+LK
Sbjct: 7   ILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRHGDHLTGLSNLK 66

Query: 63  TYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGV 122
           TY   FGYIPN +P SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG+
Sbjct: 67  TYLNKFGYIPN-SP-SNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGI 126

Query: 123 ADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSA 182
           ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+ 
Sbjct: 127 ADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTD 186

Query: 183 EVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSP 242
           EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFSP
Sbjct: 187 EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSP 246

Query: 243 PSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 302
           PSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR
Sbjct: 247 PSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 306

Query: 303 TRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTSKNGAATLLSLWWGPL 362
           TRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD SK GA  +L+ WW PL
Sbjct: 307 TRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSKGGAPKVLNSWWSPL 366

Query: 363 LLTAVELFLMR 370
           ++ AVELFLMR
Sbjct: 367 VVAAVELFLMR 372

BLAST of MC08g1511 vs. ExPASy TrEMBL
Match: A0A6J1G2R8 (metalloendoproteinase 5-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111450264 PE=3 SV=1)

HSP 1 Score: 568 bits (1465), Expect = 8.16e-202
Identity = 288/372 (77.42%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 3   VLLLISLSLFSPPI-INAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLK 62
           +L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCRPGD L GLS+LK
Sbjct: 7   ILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLNGLSNLK 66

Query: 63  TYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGV 122
           TY   FGYIPN +P SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG+
Sbjct: 67  TYLNKFGYIPN-SP-SNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGI 126

Query: 123 ADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSA 182
           ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+ 
Sbjct: 127 ADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTD 186

Query: 183 EVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSP 242
           EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFSP
Sbjct: 187 EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSP 246

Query: 243 PSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 302
           PSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR
Sbjct: 247 PSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSR 306

Query: 303 TRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTS-KNGAATLLSLWWGP 362
           TRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD S K GA  +L+ WW P
Sbjct: 307 TRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSP 366

Query: 363 LLLTAVELFLMR 370
           L++ AVELFLMR
Sbjct: 367 LVVAAVELFLMR 373

BLAST of MC08g1511 vs. ExPASy TrEMBL
Match: A0A5D3DJB2 (Metalloendoproteinase 2-MMP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00360 PE=3 SV=1)

HSP 1 Score: 555 bits (1431), Expect = 7.89e-197
Identity = 279/371 (75.20%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   FIVLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 60
           F+++  +SL +F  PI +A +FPN S+IPSWLN T C WDQF+KLKGCRPGDRL GLS+L
Sbjct: 3   FLLITSLSLLVFLNPI-SADIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRLNGLSNL 62

Query: 61  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 120
           KTY  HFGYIP   P+ NFTDLFD +L SAL TYQ NFNLN +G LDD TL QIQLPRCG
Sbjct: 63  KTYLNHFGYIP---PSPNFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCG 122

Query: 121 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 180
           + DIIN S+SMNSG  + H       FHSVSHY+FFPG+P WPA RRDLTYAFAPEN LS
Sbjct: 123 IPDIINNSTSMNSGRHSPH-------FHSVSHYTFFPGRPIWPAHRRDLTYAFAPENPLS 182

Query: 181 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 240
            EVKAVF RAF RWAAVTPLTF+ V+SFR ADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 183 NEVKAVFARAFARWAAVTPLTFSAVESFRTADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 242

Query: 241 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLDGDESWVVSGDL +AP AAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 243 PPSGHFHLDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 302

Query: 301 RTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAA-TLLSLWWGPL 360
           RTRKVDLAADD+ G+QELYGGNPNG+ S  ++    SVQERD S+NGAA  LL  WW PL
Sbjct: 303 RTRKVDLAADDINGIQELYGGNPNGNLSPPSSSMTPSVQERDMSRNGAAHRLLGFWWSPL 362

Query: 361 LLTAVELFLMR 370
           L+ AVELFLMR
Sbjct: 363 LIAAVELFLMR 362

BLAST of MC08g1511 vs. ExPASy TrEMBL
Match: A0A1S3CPI6 (metalloendoproteinase 2-MMP OS=Cucumis melo OX=3656 GN=LOC103503333 PE=3 SV=1)

HSP 1 Score: 555 bits (1431), Expect = 7.89e-197
Identity = 279/371 (75.20%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   FIVLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 60
           F+++  +SL +F  PI +A +FPN S+IPSWLN T C WDQF+KLKGCRPGDRL GLS+L
Sbjct: 3   FLLITSLSLLVFLNPI-SADIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRLNGLSNL 62

Query: 61  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 120
           KTY  HFGYIP   P+ NFTDLFD +L SAL TYQ NFNLN +G LDD TL QIQLPRCG
Sbjct: 63  KTYLNHFGYIP---PSPNFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCG 122

Query: 121 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 180
           + DIIN S+SMNSG  + H       FHSVSHY+FFPG+P WPA RRDLTYAFAPEN LS
Sbjct: 123 IPDIINNSTSMNSGRHSPH-------FHSVSHYTFFPGRPIWPAHRRDLTYAFAPENPLS 182

Query: 181 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 240
            EVKAVF RAF RWAAVTPLTF+ V+SFR ADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 183 NEVKAVFARAFARWAAVTPLTFSAVESFRTADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 242

Query: 241 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLDGDESWVVSGDL +AP AAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 243 PPSGHFHLDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 302

Query: 301 RTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAA-TLLSLWWGPL 360
           RTRKVDLAADD+ G+QELYGGNPNG+ S  ++    SVQERD S+NGAA  LL  WW PL
Sbjct: 303 RTRKVDLAADDINGIQELYGGNPNGNLSPPSSSMTPSVQERDMSRNGAAHRLLGFWWSPL 362

Query: 361 LLTAVELFLMR 370
           L+ AVELFLMR
Sbjct: 363 LIAAVELFLMR 362

BLAST of MC08g1511 vs. ExPASy TrEMBL
Match: A0A0A0KRY9 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G484630 PE=3 SV=1)

HSP 1 Score: 552 bits (1422), Expect = 2.14e-195
Identity = 277/371 (74.66%), Postives = 307/371 (82.75%), Query Frame = 0

Query: 1   FIVLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 60
           F+++  +SL LF  PI +AH+FPN S+IPSWLN T C WDQF+KLKGCRPGDR  GLS+L
Sbjct: 3   FLLITSLSLLLFLNPI-SAHIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRFNGLSNL 62

Query: 61  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 120
           KTY   FGYIP+   + NFTDLFD +L SAL TYQ NFNLN +G LDD TL QIQLPRCG
Sbjct: 63  KTYLNRFGYIPS---SPNFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCG 122

Query: 121 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 180
           + DIIN S+SMNSG P       S  FHSVSHYSFFPG+P WPA RRDLTYAFAPEN LS
Sbjct: 123 IPDIINNSTSMNSGQPTGRQ---SPHFHSVSHYSFFPGRPIWPAHRRDLTYAFAPENPLS 182

Query: 181 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 240
            EVKAVF RAF RWAAVTPLTF+ V+SFR+ADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 183 NEVKAVFARAFARWAAVTPLTFSAVESFRSADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 242

Query: 241 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLDGDESWVVSGDL +AP AAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 243 PPSGHFHLDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 302

Query: 301 RTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAATL-LSLWWGPL 360
           RTRKVDLAADD+ G+QELYGGNPNG+ S  ++    SVQER+ S+NGAA   L  WW P+
Sbjct: 303 RTRKVDLAADDINGIQELYGGNPNGNLSPPSSSTTPSVQEREMSRNGAAPRPLRFWWSPV 362

Query: 361 LLTAVELFLMR 370
           L+ AVELFLMR
Sbjct: 363 LIAAVELFLMR 366

BLAST of MC08g1511 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 4.7e-112
Identity = 202/354 (57.06%), Postives = 251/354 (70.90%), Query Frame = 0

Query: 2   IVLLLISLSLFSPPIINAHLFPNASAIPSWL--NNTKCLWDQFQKLKGCRPGDRLQGLSS 61
           I + ++ L  F+P  ++A  + N+SAIP  L  N T   W+ F    GC  G +  GL  
Sbjct: 4   ICVFMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYDGLYM 63

Query: 62  LKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRC 121
           LK YF+HFGYI   N + NFTD FDDIL +A+  YQ+NF LNV+G LD+ TL+ + +PRC
Sbjct: 64  LKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVVIPRC 123

Query: 122 GVADIINGSSSMNSGNPAAHA--ASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPEN 181
           G  D++NG+S+M+SG        A    RFH+V HYSFFPG+PRWP  RRDLTYAF P N
Sbjct: 124 GNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFDPRN 183

Query: 182 QLSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAH 241
            L+ EVK+VF RAF RW  VTPLTFT V+ F  +DI IGF++G+HGDGEPFDG + TLAH
Sbjct: 184 ALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAH 243

Query: 242 AFSPPSGHFHLDGDESWVVSGDLG---TAPAAAIDLESVAVHEIGHLLGLGHSSVEESIM 301
           AFSPP+GHFHLDG+E+W+VSG+ G    + + A+DLESVAVHEIGHLLGLGHSSVE SIM
Sbjct: 244 AFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIM 303

Query: 302 FPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGA 349
           +PTI +  RKVDL  DDV GVQ LYG NPN + S +   PP S Q+RDT  +GA
Sbjct: 304 YPTIRTGRRKVDLTTDDVEGVQYLYGANPNFNGSRS---PPPSTQQRDTGDSGA 354

BLAST of MC08g1511 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 391.0 bits (1003), Expect = 1.1e-108
Identity = 206/354 (58.19%), Postives = 248/354 (70.06%), Query Frame = 0

Query: 1   FIVLLLISLSLFSPPIINAHLFPNASAI-PSWLNNTKCLWDQFQKLKGCRPGDRLQGLSS 60
           F V   +SL L   P  +A  FPN++A+ PS  N T+  WD F    GC  G  + GL  
Sbjct: 3   FCVFGFLSLFLIVSP-ASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYR 62

Query: 61  LKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRC 120
           +K YF+ FGYIP    + NFTD FDDIL +A+  YQ NFNLNV+GELD  T++ I +PRC
Sbjct: 63  IKKYFQRFGYIPE-TFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRC 122

Query: 121 GVADIINGSSSMNSGNPAAHAASGS-ARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQ 180
           G  D++NG+S M+ G       + S    H+V  Y+ FPG+PRWP  RRDLTYAF P+N 
Sbjct: 123 GNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNP 182

Query: 181 LSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHA 240
           L+ EVK+VF RAFGRW+ VT L FT  +SF  +DI IGF+ GDHGDGEPFDGVLGTLAHA
Sbjct: 183 LTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHA 242

Query: 241 FSPPSGHFHLDGDESWVVSGDLGT--APAAAIDLESVAVHEIGHLLGLGHSSVEESIMFP 300
           FSPPSG FHLD DE+WVVSGDL +  +  AA+DLESVAVHEIGHLLGLGHSSVEESIM+P
Sbjct: 243 FSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYP 302

Query: 301 TITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQ-ERDTSKNGAA 350
           TIT+  RKVDL  DDV G+Q LYG NPN +    TT PPS+ + +RDT    AA
Sbjct: 303 TITTGKRKVDLTNDDVEGIQYLYGANPNFNG---TTSPPSTTKHQRDTGGFSAA 351

BLAST of MC08g1511 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 369.4 bits (947), Expect = 3.4e-102
Identity = 193/358 (53.91%), Postives = 236/358 (65.92%), Query Frame = 0

Query: 3   VLLLISLSLFSPPIINAHLFPNASAIP--SWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 62
           +LL I +  F+   I+A  + N S+IP   +LN T+  W+ F KL GC  G+ + GLS L
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKL 63

Query: 63  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 122
           K YF+ FGYI       N TD FDD+L SA+ TYQKNFNL V+G+LD  TLRQI  PRCG
Sbjct: 64  KQYFRRFGYI---TTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 123

Query: 123 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 182
             D+I+G S MN G              +   YSFFPGKPRWP  +RDLTYAFAP+N L+
Sbjct: 124 NPDLIDGVSEMNGGK----------ILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLT 183

Query: 183 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 242
            EVK VF RAF RWA VTPL FT  +S   ADI IGFF+G+HGDGEPFDG +GTLAHA S
Sbjct: 184 DEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASS 243

Query: 243 PPSGHFHLDGDESWVVS-GDLG---TAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFP 302
           PP+G  HLDGDE W++S G++          +DLESVAVHEIGHLLGLGHSSVE++IMFP
Sbjct: 244 PPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFP 303

Query: 303 TITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAATLLSL 355
            I+   RKV+LA DD+ G+Q LYGGNPNGD   +     S     D+ +     ++SL
Sbjct: 304 AISGGDRKVELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQSTGGDSVRRWRGWMISL 348

BLAST of MC08g1511 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.5e-76
Identity = 147/317 (46.37%), Postives = 195/317 (61.51%), Query Frame = 0

Query: 39  WDQFQKLKGCRPGDRLQGLSSLKTYFKHFGYIPNPNPNSN-FTDLFDDILHSALTTYQKN 98
           W  F +L   + G  + G+S LK Y   FGY+   N  S  F+D+FD  L SA++ YQ+N
Sbjct: 52  WHDFSRLVDVQIGSHVSGVSELKRYLHRFGYV---NDGSEIFSDVFDGPLESAISLYQEN 111

Query: 99  FNLNVSGELDDDTLRQIQLPRCGVADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFP 158
             L ++G LD  T+  + LPRCGV+D               H    +   H+ +HY++F 
Sbjct: 112 LGLPITGRLDTSTVTLMSLPRCGVSD--------------THMTINNDFLHTTAHYTYFN 171

Query: 159 GKPRWPAGRRDLTYAFAPENQL----SAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADI 218
           GKP+W   R  LTYA +  ++L    S +VK VF RAF +W++V P++F EV  F  AD+
Sbjct: 172 GKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADL 231

Query: 219 RIGFFAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDGDESWVVSGDLGTAPAAAIDLESV 278
           +IGF+AGDHGDG PFDGVLGTLAHAF+P +G  HLD  E+W+V  DL  +   A+DLESV
Sbjct: 232 KIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESV 291

Query: 279 AVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTM 338
           A HEIGHLLGLGHSS E ++M+P++  RT+KVDL  DDVAGV +LYG NP          
Sbjct: 292 ATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPNPK-------LR 342

Query: 339 PPSSVQERDTSKNGAAT 351
             S  Q  D+ KNG  +
Sbjct: 352 LDSLTQSEDSIKNGTVS 342

BLAST of MC08g1511 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.1e-65
Identity = 133/276 (48.19%), Postives = 172/276 (62.32%), Query Frame = 0

Query: 57  LSSLKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQL 116
           +  +K + + +GY+P  N  S+     D     AL  YQKN  L ++G+ D DTL QI L
Sbjct: 50  IPEIKRHLQQYGYLPQ-NKESD-----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILL 109

Query: 117 PRCGVADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRD----LTYA 176
           PRCG  D +                  +A FH+   Y +FPG+PRW    RD    LTYA
Sbjct: 110 PRCGFPDDVEPK---------------TAPFHTGKKYVYFPGRPRW---TRDVPLKLTYA 169

Query: 177 FAPEN----QLSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPF 236
           F+ EN        +++ VF RAFG+WA+V P++F E + +  ADI+IGFF GDHGDGEPF
Sbjct: 170 FSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPF 229

Query: 237 DGVLGTLAHAFSPPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSS 296
           DGVLG LAH FSP +G  HLD  E+W V  D     + A+DLESVAVHEIGH+LGLGHSS
Sbjct: 230 DGVLGVLAHTFSPENGRLHLDKAETWAVDFD-EEKSSVAVDLESVAVHEIGHVLGLGHSS 289

Query: 297 VEESIMFPTITSRTRKVDLAADDVAGVQELYGGNPN 325
           V+++ M+PT+  R++KV+L  DDV GVQ LYG NPN
Sbjct: 290 VKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTNPN 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5XF516.7e-11157.06Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
O045291.5e-10758.19Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q9ZUJ54.8e-10153.91Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
O235077.7e-7546.37Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
P291363.8e-7448.38Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022999578.18.06e-20277.36metalloendoproteinase 2-MMP [Cucurbita maxima][more]
XP_022946065.11.69e-20177.42metalloendoproteinase 5-MMP-like [Cucurbita moschata][more]
KAG7029995.12.39e-20177.42Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_023546725.11.96e-20077.15metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo][more]
XP_038889498.11.16e-19976.96metalloendoproteinase 2-MMP [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1KK373.90e-20277.36metalloendoproteinase 2-MMP OS=Cucurbita maxima OX=3661 GN=LOC111493905 PE=3 SV=... [more]
A0A6J1G2R88.16e-20277.42metalloendoproteinase 5-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111450264 P... [more]
A0A5D3DJB27.89e-19775.20Metalloendoproteinase 2-MMP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3CPI67.89e-19775.20metalloendoproteinase 2-MMP OS=Cucumis melo OX=3656 GN=LOC103503333 PE=3 SV=1[more]
A0A0A0KRY92.14e-19574.66ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G484630 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G24140.14.7e-11257.06Matrixin family protein [more]
AT1G70170.11.1e-10858.19matrix metalloproteinase [more]
AT1G59970.13.4e-10253.91Matrixin family protein [more]
AT4G16640.15.5e-7646.37Matrixin family protein [more]
AT2G45040.11.1e-6548.19Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 187..202
score: 52.52
coord: 114..127
score: 44.6
coord: 211..239
score: 61.56
coord: 273..298
score: 56.85
coord: 307..320
score: 58.8
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 158..321
e-value: 3.9E-46
score: 169.3
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 166..320
e-value: 1.0E-47
score: 161.9
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 47..326
e-value: 3.0E-77
score: 261.3
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 57..113
e-value: 2.9E-9
score: 37.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 319..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 321..343
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 3..352
NoneNo IPR availablePANTHERPTHR10201:SF260MATRIX METALLOPROTEINASEcoord: 3..352
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 155..323
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 161..320
e-value: 1.09596E-68
score: 211.294
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 49..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1511.1MC08g1511.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity