MC08g1370 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g1370
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectate lyase
Location	MC08: 13220294 .. 13222436 (+)
RNA-Seq Expression	MC08g1370
Synteny	MC08g1370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAGCAATGTTTCCAGCTTAGAGCAATAAATAAAAAAAAAAAGAACCCTCAGTTTTGGTCTGGCAAGGTCTTGAGTTTTACTTTGGCCAAAATTAAAACCTATCTGGGGTTTTTCCAAAGACCTTGACCAGCCCAAAAAAGCTTGCTTTGCTGAAAAAGCCAAAAAAAAAAAAAAAAAAAAAAAGGACACAACCTCCAGAAAACAACCAGAAAAAAGAGGTCAATTTTAAAGAAAGATGGCTGGTTTTAGAGTTGAGTTCTTGATGCTTTTGGTTTCGGTGGCTGTGATTCTCCCAACTCTCAGAGCTGGGATTGCTGAATATGATGAGTACTTGCTGCAAAAGGCTGAAGAAGCCAAAATGGCTTCTCTTGAGGCCTTCCATTCTGACCCCATGAATGTCACTGACCACTTCAACCTTCAAGTCCATTTGTATGTCCAAATTTTCTACCTTTTTTTTTATCTTTCGTTTTGCGGTTTAATAATATTTAGGTGTTTTTTGGTCAAAATTGTGTGTTGATTTGTTAAATTTTGGGTGGCAGAGCTTTGGAGGGGAGCAATAGCACGAGGAGGAGCATGAAGAAATACAACGGGCCGTGCATGGCCACAAACCCCATCGACCGGTGCTGGCGTTGCGACCCAAACTGGGCCAACAACCGTAAGAAACTTGCCACTTGTGTGCTTGGTTTTGGCCGAAAAACCCGAGGCGGTGTGAAGGGTAAGATCTATGTCGTCACTGATCCCTCTGACGCTGATGTCGTAAACCCTAAACCTGGAACCCTAAGATACGGGGCAGTCCAGAAGAAGCCATTGTGGATCATCTTTGGTCGGAGCATGATCATTACACTGTCCAAAGAGCTGCTTGTGGCCAGCCATAAGACCATCGATGCCCGCGGCGCCAATGTCCACATTGCCTTCGGTGCTGGTATCAGCATCCAATTCGCCAAGAATGTGATCATCCACGGCCTCCACATCCACCACATTATCGTTGCCACCGGCGGTCTGATCAGGGACTCCACCAACCATGTTGGCCTCCGAACAAACAGCGACGGTGACGGCGTCTCCATCTTCGGTTCCACCAACATCTGGCTCGACCATCTCTCCATGTCCAACTGCGCGGACGGACTCATCGACGCAATCCAAGGCTCCACTGCCATCACCATCTCAAACTCCCATTTCACCCATCACAACGACGTACCGAACCATATCCCTCTATCTTAACTTCGTCGCATTCCAACTTCTGAAACAAAATTTATATTTATTACTTTGTATCGCAGGTGATGTTGTTTGGTGCGAGCGACTCGTATCAGGGCGACTCAGTCATGCAAGTGACAGTAGCGTTCAACCATTTCGGTAAGGGATTGGTGCAGAGGATGCCCAGATGCAGATGGGGATTCTTCCACGTCGTGAACAACGATTACACCCACTGGCTCATGTACGCCATTGGTGGCAGCCAACACCCCACCATTATCAGCCAGGGCAATCGCTTCATTGCCCCACCCAACCCTGCCGCAAAACAGGTTCGTTTCATTTCTGAAGCCTAAAGTTTAAGCAGTTTAAGCAGATAGGTTACGATGAGTAAATTTGTGTGTTGTTGTCGGTTGCAGATTACAAACAGAAACTACGCGACCGAGGCTGTATGGAAGACATGGACATGGAGGTCAGAAGGAGATCTAATGATGAATGGAGCATACTTTGTGCAGTCTGGAAATCCAAGCAAACGAAGGCCATACAGCAGGCATGACATGATCAAGGCCAAGCCTGGAACTTTTGTTAGGAGGCTCACTCGTTTCTCTGGCTCACTCAACTGCTTTGTTGGTCGCCCTTGCTAAACGAACACCACCCACCCACAACACAACAACAACACAGCAATCACACCATTTTTTTTCTTTTTTTTTTTTAAATTATAAATTTCATCATTTGTTCTGAGTTTCTTTTTTACCTTTTTCTGAAAAAAAAAATAATAATAAGAGAGAAAGAGATAGAGGAAAGATAGAGAGGGTGAAGAGTCAATCAAAGCCAGCAAAGAGAAGAAGAGGAGAGAAAGAGAGAGAGAGAGGAGGGATTTAGTGTTGGTGTACTATTGATTCATTCATACATTCTTCACTTTGCTTTGGTTTCAATGAGAGATGTCCCATGTGCTTG

mRNA sequence

CAAGCAATGTTTCCAGCTTAGAGCAATAAATAAAAAAAAAAAGAACCCTCAGTTTTGGTCTGGCAAGGTCTTGAGTTTTACTTTGGCCAAAATTAAAACCTATCTGGGGTTTTTCCAAAGACCTTGACCAGCCCAAAAAAGCTTGCTTTGCTGAAAAAGCCAAAAAAAAAAAAAAAAAAAAAAAGGACACAACCTCCAGAAAACAACCAGAAAAAAGAGGTCAATTTTAAAGAAAGATGGCTGGTTTTAGAGTTGAGTTCTTGATGCTTTTGGTTTCGGTGGCTGTGATTCTCCCAACTCTCAGAGCTGGGATTGCTGAATATGATGAGTACTTGCTGCAAAAGGCTGAAGAAGCCAAAATGGCTTCTCTTGAGGCCTTCCATTCTGACCCCATGAATGTCACTGACCACTTCAACCTTCAAGTCCATTTAGCTTTGGAGGGGAGCAATAGCACGAGGAGGAGCATGAAGAAATACAACGGGCCGTGCATGGCCACAAACCCCATCGACCGGTGCTGGCGTTGCGACCCAAACTGGGCCAACAACCGTAAGAAACTTGCCACTTGTGTGCTTGGTTTTGGCCGAAAAACCCGAGGCGGTGTGAAGGGTAAGATCTATGTCGTCACTGATCCCTCTGACGCTGATGTCGTAAACCCTAAACCTGGAACCCTAAGATACGGGGCAGTCCAGAAGAAGCCATTGTGGATCATCTTTGGTCGGAGCATGATCATTACACTGTCCAAAGAGCTGCTTGTGGCCAGCCATAAGACCATCGATGCCCGCGGCGCCAATGTCCACATTGCCTTCGGTGCTGGTATCAGCATCCAATTCGCCAAGAATGTGATCATCCACGGCCTCCACATCCACCACATTATCGTTGCCACCGGCGGTCTGATCAGGGACTCCACCAACCATGTTGGCCTCCGAACAAACAGCGACGGTGACGGCGTCTCCATCTTCGGTTCCACCAACATCTGGCTCGACCATCTCTCCATGTCCAACTGCGCGGACGGACTCATCGACGCAATCCAAGGCTCCACTGCCATCACCATCTCAAACTCCCATTTCACCCATCACAACGACGTGATGTTGTTTGGTGCGAGCGACTCGTATCAGGGCGACTCAGTCATGCAAGTGACAGTAGCGTTCAACCATTTCGGTAAGGGATTGGTGCAGAGGATGCCCAGATGCAGATGGGGATTCTTCCACGTCGTGAACAACGATTACACCCACTGGCTCATGTACGCCATTGGTGGCAGCCAACACCCCACCATTATCAGCCAGGGCAATCGCTTCATTGCCCCACCCAACCCTGCCGCAAAACAGATTACAAACAGAAACTACGCGACCGAGGCTGTATGGAAGACATGGACATGGAGGTCAGAAGGAGATCTAATGATGAATGGAGCATACTTTGTGCAGTCTGGAAATCCAAGCAAACGAAGGCCATACAGCAGGCATGACATGATCAAGGCCAAGCCTGGAACTTTTGTTAGGAGGCTCACTCGTTTCTCTGGCTCACTCAACTGCTTTGTTGGTCGCCCTTGCTAAACGAACACCACCCACCCACAACACAACAACAACACAGCAATCACACCATTTTTTTTCTTTTTTTTTTTTAAATTATAAATTTCATCATTTGTTCTGAGTTTCTTTTTTACCTTTTTCTGAAAAAAAAAATAATAATAAGAGAGAAAGAGATAGAGGAAAGATAGAGAGGGTGAAGAGTCAATCAAAGCCAGCAAAGAGAAGAAGAGGAGAGAAAGAGAGAGAGAGAGGAGGGATTTAGTGTTGGTGTACTATTGATTCATTCATACATTCTTCACTTTGCTTTGGTTTCAATGAGAGATGTCCCATGTGCTTG

Coding sequence (CDS)

ATGGCTGGTTTTAGAGTTGAGTTCTTGATGCTTTTGGTTTCGGTGGCTGTGATTCTCCCAACTCTCAGAGCTGGGATTGCTGAATATGATGAGTACTTGCTGCAAAAGGCTGAAGAAGCCAAAATGGCTTCTCTTGAGGCCTTCCATTCTGACCCCATGAATGTCACTGACCACTTCAACCTTCAAGTCCATTTAGCTTTGGAGGGGAGCAATAGCACGAGGAGGAGCATGAAGAAATACAACGGGCCGTGCATGGCCACAAACCCCATCGACCGGTGCTGGCGTTGCGACCCAAACTGGGCCAACAACCGTAAGAAACTTGCCACTTGTGTGCTTGGTTTTGGCCGAAAAACCCGAGGCGGTGTGAAGGGTAAGATCTATGTCGTCACTGATCCCTCTGACGCTGATGTCGTAAACCCTAAACCTGGAACCCTAAGATACGGGGCAGTCCAGAAGAAGCCATTGTGGATCATCTTTGGTCGGAGCATGATCATTACACTGTCCAAAGAGCTGCTTGTGGCCAGCCATAAGACCATCGATGCCCGCGGCGCCAATGTCCACATTGCCTTCGGTGCTGGTATCAGCATCCAATTCGCCAAGAATGTGATCATCCACGGCCTCCACATCCACCACATTATCGTTGCCACCGGCGGTCTGATCAGGGACTCCACCAACCATGTTGGCCTCCGAACAAACAGCGACGGTGACGGCGTCTCCATCTTCGGTTCCACCAACATCTGGCTCGACCATCTCTCCATGTCCAACTGCGCGGACGGACTCATCGACGCAATCCAAGGCTCCACTGCCATCACCATCTCAAACTCCCATTTCACCCATCACAACGACGTGATGTTGTTTGGTGCGAGCGACTCGTATCAGGGCGACTCAGTCATGCAAGTGACAGTAGCGTTCAACCATTTCGGTAAGGGATTGGTGCAGAGGATGCCCAGATGCAGATGGGGATTCTTCCACGTCGTGAACAACGATTACACCCACTGGCTCATGTACGCCATTGGTGGCAGCCAACACCCCACCATTATCAGCCAGGGCAATCGCTTCATTGCCCCACCCAACCCTGCCGCAAAACAGATTACAAACAGAAACTACGCGACCGAGGCTGTATGGAAGACATGGACATGGAGGTCAGAAGGAGATCTAATGATGAATGGAGCATACTTTGTGCAGTCTGGAAATCCAAGCAAACGAAGGCCATACAGCAGGCATGACATGATCAAGGCCAAGCCTGGAACTTTTGTTAGGAGGCTCACTCGTTTCTCTGGCTCACTCAACTGCTTTGTTGGTCGCCCTTGCTAA

Protein sequence

MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVHLALEGSNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRLTRFSGSLNCFVGRPC

Homology

[Close]

BLAST of MC08g1370 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 8.3e-161
Identity = 272/468 (58.12%), Postives = 341/468 (72.86%), Query Frame = 0


Query: 5   RVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQ-- 64
           R+  LM    +AV++PT+R  I+E DEY  Q+A+EA+  +L+A+HSDP  + DHF+ +  
Sbjct: 5   RLSKLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHY 64

Query: 65  ------------------------VHLALEGSNSTRRSMK--------KYNGPCMATNPI 124
                                   + +    +NSTRRS++        K  GPC A+NPI
Sbjct: 65  DNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPI 124

Query: 125 DRCWRCDPNWANNRKKLATCVLGFGRKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAV 184
           D+CWRC  +WA  RKKL  CV GFG +T GG +G+IYVVT   D D+VNPKPGTLR+  +
Sbjct: 125 DKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVI 184

Query: 185 QKKPLWIIFGRSMIITLSKELLVASHKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIH 244
           QK+PLWIIF   M I L++ELL+ SHKTIDARGANVH+A GAGI++QF KNVIIHGLHIH
Sbjct: 185 QKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIH 244

Query: 245 HIIVATGGLIRDSTNHVGLRTNSDGDGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAI 304
           HI  ++GG+IRDS +H G+RT +DGDG+SI+GS+NIWLDH+SMS C DGLIDAI GST I
Sbjct: 245 HISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGI 304

Query: 305 TISNSHFTHHNDVMLFGASDSYQGDSVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDY 364
           TISNSHFTHHNDVML GA ++ + D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDY
Sbjct: 305 TISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDY 364

Query: 365 THWLMYAIGGSQHPTIISQGNRFIAPPN-PAAKQITNRNYATEAVWKTWTWRSEGDLMMN 424
           THW +YAIGGSQ PTI+S GNRFIAPP+ P  +++T R+YA+E  WK W WRS+ D+ MN
Sbjct: 365 THWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMN 424

Query: 425 GAYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           GAYF QSGNP  +  ++R  MIK K G  V +LT+++G+L+C VGR C
Sbjct: 425 GAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of MC08g1370 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 4.5e-159
Identity = 264/415 (63.61%), Postives = 326/415 (78.55%), Query Frame = 0


Query: 24  AGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVHLALEGSNSTRRSMKKYN-G 83
           A IAE+DEY  +K++ A+  + +A+   P  VT+HFN  VH + EG NSTRR+++    G
Sbjct: 22  ANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NSTRRNLRTNKLG 81

Query: 84  PCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRGGVKGKIYVVTDPSDADVVNPKP 143
            C+ATNPIDRCWRC  NW+ NRK L  CV GFGRKT GG  G+IYVVTDPSD  + +PK 
Sbjct: 82  QCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDSLTDPKF 141

Query: 144 GTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTIDARGANVHIAFGAGISIQFAKNV 203
           GTLR+G +Q +PLWIIFG+SM+I L +EL++ + KTID RGANV IA GA +++QF  NV
Sbjct: 142 GTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTVQFVHNV 201

Query: 204 IIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSIFGSTNIWLDHLSMSNCADGLID 263
           IIHG+HIH I    GGLIRDS  H G+RT SDGDG+SI GS+NIW+DH+S++ C+DGLID
Sbjct: 202 IIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARCSDGLID 261

Query: 264 AIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQVTVAFNHFGKGLVQRMPRCRWGF 323
            I GSTAITISN H T H+DVML GASD+Y  D +MQVTVAFNHFG+GLVQRMPRCR+GF
Sbjct: 262 VILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMPRCRYGF 321

Query: 324 FHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAKQITNRNYATEAVWKTWTWRS 383
            HVVNNDYTHW+MYA+GGSQHPTIISQGNR+IAP   AAK++T R+YA  A W  WTW+S
Sbjct: 322 VHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWSKWTWKS 381

Query: 384 EGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           +GDL ++GA+FV+SG P + + YS+ D+IKAKPGTFV+RLTRFSG+LNC     C
Sbjct: 382 QGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFSGALNCKENMEC 434

[Close]

BLAST of MC08g1370 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 6.6e-158
Identity = 267/467 (57.17%), Postives = 336/467 (71.95%), Query Frame = 0


Query: 9   LMLLVSVAVILPTLRAGIA-EYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQ----- 68
           L+ ++ +A ++PT+RA +A E DEY + KA EA+  +L A+H DP  + DHF+ +     
Sbjct: 9   LVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNS 68

Query: 69  -------------------VHLALEGSNSTRRSM------------KKYNGPCMATNPID 128
                              + +    +NSTRRS+             K  GPC A+NPID
Sbjct: 69  TDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPID 128

Query: 129 RCWRCDPNWANNRKKLATCVLGFGRKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQ 188
           +CWRC P+WA  RKKL  CV GFG +T GG +G+IYVVT P D D+VNP+PGTLR+  +Q
Sbjct: 129 KCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQ 188

Query: 189 KKPLWIIFGRSMIITLSKELLVASHKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIHH 248
           K+PLWI+F   M I LS+EL++ S KTIDARGANVHIA+GAGI++Q+  N+IIHGLH+HH
Sbjct: 189 KEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHH 248

Query: 249 IIVATGGLIRDSTNHVGLRTNSDGDGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAIT 308
           I+ ++GGLIRDS NH G R  +DGDG+SIFG+TNIWLDH+SMS C DGLIDAI GSTAIT
Sbjct: 249 IVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAIT 308

Query: 309 ISNSHFTHHNDVMLFGASDSYQGDSVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYT 368
           ISNSHFTHHNDVML GA ++   D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDYT
Sbjct: 309 ISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYT 368

Query: 369 HWLMYAIGGSQHPTIISQGNRFIAPPNPA-AKQITNRNYATEAVWKTWTWRSEGDLMMNG 428
           HW +YAIGGSQ PTI+S GNRFIAPP+    +++T R+YA+E+ WK W WRSE D+ MN 
Sbjct: 369 HWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNN 428

Query: 429 AYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           AYF QSGNP  +  +SR  MIK K G  V +LT+++G+L+C VG+ C
Sbjct: 429 AYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of MC08g1370 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 2.3e-155
Identity = 264/444 (59.46%), Postives = 324/444 (72.97%), Query Frame = 0


Query: 5   RVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVH 64
           +  FL L ++ A I+P+L A I  YDE   ++AEEAK  +   +   P NVT  FN ++ 
Sbjct: 7   KYSFLFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLR 66

Query: 65  LALE--------GSNSTRRSM--KKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGF 124
             ++         +NSTRR +  KKY GPCM TNPID+CWRCDPNWA+NRKKLA C +GF
Sbjct: 67  DTMKELKKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGF 126

Query: 125 GRKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVA 184
           G K  GG  G+ YVVTD SD D  +PKPGTLR+  +QK+PLWIIF R M I L +E+++ 
Sbjct: 127 GSKAIGGKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQ 186

Query: 185 SHKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSD 244
           S KTIDARG NVHI  GAGI++Q+ KNVIIHGLHIH I+   GG++RD+ +H+G+RT SD
Sbjct: 187 SDKTIDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSD 246

Query: 245 GDGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQG 304
           GDG+SIFG++ IW+DH+SM  C DGLIDA++GST ITISN HFT HN+VMLFGASDS   
Sbjct: 247 GDGISIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSI 306

Query: 305 DSVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFI 364
           D VMQ+T+AFNHFGK L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HPTII QGNRFI
Sbjct: 307 DQVMQITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFI 366

Query: 365 APPNPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPS-KRRPYSRHDMIKA 424
           APP+   KQ+T R Y  E+VW  WTWRSEG+L MNGAYF +SG+P    +    +D I A
Sbjct: 367 APPDIFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISA 426

Query: 425 KPGTFVRRLTRFSGSLNCFVGRPC 438
            P   V  +TRF+G L C  G+PC
Sbjct: 427 APAEDVTWMTRFAGVLGCKPGKPC 449

[Close]

BLAST of MC08g1370 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.0e-151
Identity = 256/447 (57.27%), Postives = 318/447 (71.14%), Query Frame = 0


Query: 8   FLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVHLAL 67
           F+     +A++ P +R  +A +D Y  Q+  +A   ++ ++   P+NVT+HFN  V++A+
Sbjct: 13  FVFFSSFLAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAV 72

Query: 68  EGS---NSTRRSM---------KKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFG 127
           + S   N TRR +          K +G C+A NPID CWRCD NWANNRKKLA CVLGFG
Sbjct: 73  DASESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFG 132

Query: 128 RKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVAS 187
           R+T GG  G IYVV D SD D++NPKPGTLR+   +  PLWIIF RSMII L +EL++ S
Sbjct: 133 RRTTGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITS 192

Query: 188 HKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDG 247
            KTID RGA V+I  GAG+++QF  NVIIH +++ HI+   GGLIRDS  H+GLRT SDG
Sbjct: 193 DKTIDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDG 252

Query: 248 DGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGD 307
           DG+S+FG+TNIW+DH+SM+ CADG+IDAI GSTA+TISNSHFT H +VMLFGA D +  D
Sbjct: 253 DGISLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVID 312

Query: 308 SVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIA 367
             MQ+TVAFNHFGK L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIA
Sbjct: 313 KKMQITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIA 372

Query: 368 PPNPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSG-----NPSKRRPYSRHDM 427
           PPN  AKQIT R Y     WK+W W+SEGD  +NGAYFVQSG     +   + P      
Sbjct: 373 PPNEEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFT 432

Query: 428 IKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           I+ KPGT VR+LT  +G L C +G  C
Sbjct: 433 IRPKPGTMVRKLTMDAGVLGCKLGEAC 459

[Close]

BLAST of MC08g1370 vs. NCBI nr
Match: XP_022152137.1 (probable pectate lyase 19 [Momordica charantia])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0


Query: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60
           MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN
Sbjct: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60

Query: 61  LQVHLALEGSNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRG 120
           LQVHLALEGSNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRG
Sbjct: 61  LQVHLALEGSNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRG 120

Query: 121 GVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTID 180
           GVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTID
Sbjct: 121 GVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTID 180

Query: 181 ARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSI 240
           ARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSI
Sbjct: 181 ARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSI 240

Query: 241 FGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQV 300
           FGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQV
Sbjct: 241 FGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQV 300

Query: 301 TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA 360
           TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA
Sbjct: 301 TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA 360

Query: 361 AKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVR 420
           AKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVR
Sbjct: 361 AKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVR 420

Query: 421 RLTRFSGSLNCFVGRPC 437
           RLTRFSGSLNCFVGRPC
Sbjct: 421 RLTRFSGSLNCFVGRPC 437

[Close]

BLAST of MC08g1370 vs. NCBI nr
Match: XP_004147462.2 (probable pectate lyase 7 [Cucumis sativus] >KGN59593.1 hypothetical protein Csa_001450 [Cucumis sativus])

HSP 1 Score: 811 bits (2095), Expect = 3.24e-295
Identity = 382/440 (86.82%), Postives = 413/440 (93.86%), Query Frame = 0


Query: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60
           M G +++ +M+LVS+AV++PTLRAGIAE+D++LLQK+EEAK ASLEAFH DPMNVTDHFN
Sbjct: 2   MTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFN 61

Query: 61  LQVHLALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRK 120
             VHLALEG   SNSTRRS+ KYNGPC+ATNPIDRCWRCDPNWA NRKKLA CVLGFGRK
Sbjct: 62  QHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRK 121

Query: 121 TRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHK 180
           T GG KG+IYVVTDPSD DV+NP+PGTLRYGA+QKKPLWIIF RSMII LSKEL++ SHK
Sbjct: 122 TTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHK 181

Query: 181 TIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDG 240
           TIDARGANVHIA+GAG+SIQFA+NVIIHGL IHH+I   GG+IRD+ NHVGLRT SDGDG
Sbjct: 182 TIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDG 241

Query: 241 VSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSV 300
           +SIFGSTNIWLDHLSMSNC DGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS+
Sbjct: 242 ISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSI 301

Query: 301 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 360
           MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP
Sbjct: 302 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 361

Query: 361 NPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGT 420
           NPAA+QITNRNYATE+VWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSR DMIKAKPGT
Sbjct: 362 NPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGT 421

Query: 421 FVRRLTRFSGSLNCFVGRPC 437
           FVRRLTRFSGSLNC+VGRPC
Sbjct: 422 FVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of MC08g1370 vs. NCBI nr
Match: XP_008443432.1 (PREDICTED: probable pectate lyase 19 [Cucumis melo])

HSP 1 Score: 809 bits (2090), Expect = 1.87e-294
Identity = 383/439 (87.24%), Postives = 411/439 (93.62%), Query Frame = 0


Query: 2   AGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNL 61
           AG +++ LM+LVS+AV++PTLRAGIAE+D+YLLQKAEEAK ASLEAFH DPMNVTDHFN 
Sbjct: 3   AGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHFNQ 62

Query: 62  QVHLALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKT 121
            VHLALEG   SNSTRRS+ KY GPC+ATNPIDRCWRCDPNWA NRKKLA CVLGFGRKT
Sbjct: 63  HVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKT 122

Query: 122 RGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKT 181
            GG KG+IYVVTDPSD DVVNPKPGTLRYGA+QK+PLWIIF RSMII LSKEL+V SHKT
Sbjct: 123 TGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSHKT 182

Query: 182 IDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGV 241
           IDARG+NVHIA+GAG+SIQFA+NVIIHGL IHH+I   GG+IRD+ NHVGLRT SDGDG+
Sbjct: 183 IDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGI 242

Query: 242 SIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVM 301
           SIFGS+NIWLDHLSMSNC DGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS+M
Sbjct: 243 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIM 302

Query: 302 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 361
           QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN
Sbjct: 303 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 362

Query: 362 PAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTF 421
           PAA+QITNRNYA E+VWKTWTWRSEGDLMMNGAYF+QSGNPSKRRPYSR DMIKAKPGTF
Sbjct: 363 PAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPGTF 422

Query: 422 VRRLTRFSGSLNCFVGRPC 437
           VRRLTRFSGSLNCFVGRPC
Sbjct: 423 VRRLTRFSGSLNCFVGRPC 441

[Close]

BLAST of MC08g1370 vs. NCBI nr
Match: XP_038905811.1 (pectate lyase-like [Benincasa hispida])

HSP 1 Score: 799 bits (2063), Expect = 2.43e-290
Identity = 373/435 (85.75%), Postives = 410/435 (94.25%), Query Frame = 0


Query: 6   VEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVHL 65
           ++ +M LVS+AV++PTLRAGIAEYD+YLLQKAEEAK A+LE+FH DPMNVTDHFN  VHL
Sbjct: 7   IKVVMFLVSMAVLMPTLRAGIAEYDDYLLQKAEEAKQAALESFHPDPMNVTDHFNEHVHL 66

Query: 66  ALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRGGV 125
           ALEG   SNSTRRS+KKYNGPCMATNPIDRCWRCD NWA NRKKL +CVLGFGRKT GG+
Sbjct: 67  ALEGIEGSNSTRRSLKKYNGPCMATNPIDRCWRCDRNWAMNRKKLVSCVLGFGRKTTGGL 126

Query: 126 KGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTIDAR 185
           +G+ YVVTDPSD DV+NP+ GTLR+ A+QK+PLWIIFGRSMII LSKELL+ SHKTIDAR
Sbjct: 127 RGRFYVVTDPSDNDVINPRRGTLRHAAIQKRPLWIIFGRSMIIRLSKELLITSHKTIDAR 186

Query: 186 GANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSIFG 245
           GANVHIA+GAG+SIQF++NVIIHGLHIHH++ A GG+IRDS +HVGLRT SDGDG+SIFG
Sbjct: 187 GANVHIAYGAGLSIQFSRNVIIHGLHIHHVVSARGGMIRDSADHVGLRTVSDGDGISIFG 246

Query: 246 STNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQVTV 305
           S+NIWLDHLSMSNC DGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS+MQVTV
Sbjct: 247 SSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQVTV 306

Query: 306 AFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAK 365
           AFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAA+
Sbjct: 307 AFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAR 366

Query: 366 QITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRL 425
           Q+TNRNYATE+VW+TWTWRSEGDLMMNGAYF+QSGNPSKRRPYSR DMIKAKPGTFVRRL
Sbjct: 367 QVTNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPGTFVRRL 426

Query: 426 TRFSGSLNCFVGRPC 437
           TRFSGSLNC+VGRPC
Sbjct: 427 TRFSGSLNCYVGRPC 441

[Close]

BLAST of MC08g1370 vs. NCBI nr
Match: KAG7017718.1 (putative pectate lyase P59 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 778 bits (2008), Expect = 5.83e-282
Identity = 363/440 (82.50%), Postives = 411/440 (93.41%), Query Frame = 0


Query: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60
           MAG+ ++  +LL+S+AV++P+LRAGIAEYD++LLQ+AEEAK A+L+A+H  PMNVTDHFN
Sbjct: 2   MAGWSIKAALLLLSMAVLMPSLRAGIAEYDDFLLQQAEEAKQAALKAYHPHPMNVTDHFN 61

Query: 61  LQVHLALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRK 120
             V+LAL+G   +NSTRRS+++Y GPC+ATNPIDRCWRCD NWANNRKKL  CVLGFGRK
Sbjct: 62  HHVYLALQGIQGNNSTRRSLRQYKGPCLATNPIDRCWRCDRNWANNRKKLGYCVLGFGRK 121

Query: 121 TRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHK 180
           T GG++G+ Y VTD SDADVVNPKPGTLR+  VQK+PLWIIFGRSMII LSKELLV S K
Sbjct: 122 TTGGLRGRFYTVTDASDADVVNPKPGTLRHAVVQKRPLWIIFGRSMIIRLSKELLVTSDK 181

Query: 181 TIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDG 240
           TIDARGANVHIA+GAGIS+QFA+NVIIHGLHIHHI+ A+GG+IRD+ +HVGLRT SDGDG
Sbjct: 182 TIDARGANVHIAYGAGISVQFARNVIIHGLHIHHILSASGGMIRDAADHVGLRTVSDGDG 241

Query: 241 VSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSV 300
           +SIFGS+N+WLDHLSMSNC DGLIDAIQGSTAIT+SNSHFTHHNDVMLFGASDS++GDSV
Sbjct: 242 ISIFGSSNVWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSFEGDSV 301

Query: 301 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 360
           MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP
Sbjct: 302 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 361

Query: 361 NPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGT 420
           NPAAKQITNRNYATE+VWKTWTWRSEGDL+MNGAYFV+SG+PSK+RPYSR DMIKA+PGT
Sbjct: 362 NPAAKQITNRNYATESVWKTWTWRSEGDLLMNGAYFVESGDPSKQRPYSRFDMIKARPGT 421

Query: 421 FVRRLTRFSGSLNCFVGRPC 437
           FVRRLTRFSGSLNC+VGRPC
Sbjct: 422 FVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of MC08g1370 vs. ExPASy TrEMBL
Match: A0A6J1DFD2 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111019925 PE=3 SV=1)

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0


Query: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60
           MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN
Sbjct: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60

Query: 61  LQVHLALEGSNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRG 120
           LQVHLALEGSNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRG
Sbjct: 61  LQVHLALEGSNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRG 120

Query: 121 GVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTID 180
           GVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTID
Sbjct: 121 GVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTID 180

Query: 181 ARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSI 240
           ARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSI
Sbjct: 181 ARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSI 240

Query: 241 FGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQV 300
           FGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQV
Sbjct: 241 FGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQV 300

Query: 301 TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA 360
           TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA
Sbjct: 301 TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA 360

Query: 361 AKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVR 420
           AKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVR
Sbjct: 361 AKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVR 420

Query: 421 RLTRFSGSLNCFVGRPC 437
           RLTRFSGSLNCFVGRPC
Sbjct: 421 RLTRFSGSLNCFVGRPC 437

[Close]

BLAST of MC08g1370 vs. ExPASy TrEMBL
Match: A0A0A0LCU3 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G827350 PE=3 SV=1)

HSP 1 Score: 811 bits (2095), Expect = 1.57e-295
Identity = 382/440 (86.82%), Postives = 413/440 (93.86%), Query Frame = 0


Query: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60
           M G +++ +M+LVS+AV++PTLRAGIAE+D++LLQK+EEAK ASLEAFH DPMNVTDHFN
Sbjct: 2   MTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFN 61

Query: 61  LQVHLALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRK 120
             VHLALEG   SNSTRRS+ KYNGPC+ATNPIDRCWRCDPNWA NRKKLA CVLGFGRK
Sbjct: 62  QHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRK 121

Query: 121 TRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHK 180
           T GG KG+IYVVTDPSD DV+NP+PGTLRYGA+QKKPLWIIF RSMII LSKEL++ SHK
Sbjct: 122 TTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHK 181

Query: 181 TIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDG 240
           TIDARGANVHIA+GAG+SIQFA+NVIIHGL IHH+I   GG+IRD+ NHVGLRT SDGDG
Sbjct: 182 TIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDG 241

Query: 241 VSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSV 300
           +SIFGSTNIWLDHLSMSNC DGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS+
Sbjct: 242 ISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSI 301

Query: 301 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 360
           MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP
Sbjct: 302 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 361

Query: 361 NPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGT 420
           NPAA+QITNRNYATE+VWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSR DMIKAKPGT
Sbjct: 362 NPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGT 421

Query: 421 FVRRLTRFSGSLNCFVGRPC 437
           FVRRLTRFSGSLNC+VGRPC
Sbjct: 422 FVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of MC08g1370 vs. ExPASy TrEMBL
Match: A0A1S3B8R7 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487022 PE=3 SV=1)

HSP 1 Score: 809 bits (2090), Expect = 9.06e-295
Identity = 383/439 (87.24%), Postives = 411/439 (93.62%), Query Frame = 0


Query: 2   AGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNL 61
           AG +++ LM+LVS+AV++PTLRAGIAE+D+YLLQKAEEAK ASLEAFH DPMNVTDHFN 
Sbjct: 3   AGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHFNQ 62

Query: 62  QVHLALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKT 121
            VHLALEG   SNSTRRS+ KY GPC+ATNPIDRCWRCDPNWA NRKKLA CVLGFGRKT
Sbjct: 63  HVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKT 122

Query: 122 RGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKT 181
            GG KG+IYVVTDPSD DVVNPKPGTLRYGA+QK+PLWIIF RSMII LSKEL+V SHKT
Sbjct: 123 TGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSHKT 182

Query: 182 IDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGV 241
           IDARG+NVHIA+GAG+SIQFA+NVIIHGL IHH+I   GG+IRD+ NHVGLRT SDGDG+
Sbjct: 183 IDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGI 242

Query: 242 SIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVM 301
           SIFGS+NIWLDHLSMSNC DGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS+M
Sbjct: 243 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIM 302

Query: 302 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 361
           QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN
Sbjct: 303 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 362

Query: 362 PAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTF 421
           PAA+QITNRNYA E+VWKTWTWRSEGDLMMNGAYF+QSGNPSKRRPYSR DMIKAKPGTF
Sbjct: 363 PAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPGTF 422

Query: 422 VRRLTRFSGSLNCFVGRPC 437
           VRRLTRFSGSLNCFVGRPC
Sbjct: 423 VRRLTRFSGSLNCFVGRPC 441

[Close]

BLAST of MC08g1370 vs. ExPASy TrEMBL
Match: A0A6J1F1Q5 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111441594 PE=3 SV=1)

HSP 1 Score: 776 bits (2005), Expect = 8.08e-282
Identity = 363/440 (82.50%), Postives = 410/440 (93.18%), Query Frame = 0


Query: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60
           MAG+ ++  +LL+S+A ++P+LRAGIAEYD++LLQ+AEEAK A+L+A+H  PMNVTDHFN
Sbjct: 2   MAGWSIKAALLLLSMAALMPSLRAGIAEYDDFLLQQAEEAKQAALKAYHPHPMNVTDHFN 61

Query: 61  LQVHLALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRK 120
             V+LAL+G   +NSTRRS+++Y GPC+ATNPIDRCWRCD NWANNRKKL  CVLGFGRK
Sbjct: 62  HHVYLALQGIQGNNSTRRSLRQYKGPCLATNPIDRCWRCDRNWANNRKKLGYCVLGFGRK 121

Query: 121 TRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHK 180
           T GG++G+ Y VTD SDADVVNPKPGTLR+  VQK+PLWIIFGRSMII LSKELLV S K
Sbjct: 122 TTGGLRGRFYTVTDASDADVVNPKPGTLRHAVVQKRPLWIIFGRSMIIRLSKELLVTSDK 181

Query: 181 TIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDG 240
           TIDARGANVHIA+GAGISIQFA+NVIIHGLHIHHI+ A+GG+IRD+ +HVGLRT SDGDG
Sbjct: 182 TIDARGANVHIAYGAGISIQFARNVIIHGLHIHHILSASGGMIRDAADHVGLRTVSDGDG 241

Query: 241 VSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSV 300
           +SIFGS+N+WLDHLSMSNC DGLIDAIQGSTAIT+SNSHFTHHNDVMLFGASDS++GDSV
Sbjct: 242 ISIFGSSNVWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSFEGDSV 301

Query: 301 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 360
           MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP
Sbjct: 302 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 361

Query: 361 NPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGT 420
           NPAAKQITNRNYATE+VWKTWTWRSEGDL+MNGAYFV+SG+PSK+RPYSR DMIKA+PGT
Sbjct: 362 NPAAKQITNRNYATESVWKTWTWRSEGDLLMNGAYFVESGDPSKQRPYSRFDMIKARPGT 421

Query: 421 FVRRLTRFSGSLNCFVGRPC 437
           FVRRLTRFSGSLNC+VGRPC
Sbjct: 422 FVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of MC08g1370 vs. ExPASy TrEMBL
Match: A0A6J1J8N7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111482295 PE=3 SV=1)

HSP 1 Score: 776 bits (2004), Expect = 1.15e-281
Identity = 361/440 (82.05%), Postives = 410/440 (93.18%), Query Frame = 0


Query: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60
           MAG+ ++  ++L+S+A+++P+LRAGIAEYD++LLQ+AEEAK A+L+A+H  PMNVT+HFN
Sbjct: 2   MAGWSIKAALVLLSMALLMPSLRAGIAEYDDFLLQQAEEAKQAALKAYHPHPMNVTEHFN 61

Query: 61  LQVHLALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRK 120
             V+LALEG   +N+TRRS++KY GPC+ATNPIDRCWRCD NWANNRKKL  CVLGFGRK
Sbjct: 62  HHVYLALEGIQGNNNTRRSLRKYKGPCLATNPIDRCWRCDQNWANNRKKLGYCVLGFGRK 121

Query: 121 TRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHK 180
           T GG++G+ Y VTD SDADVVNPKPGTLR+  VQK+PLWIIFGRSMII LSKELLV S K
Sbjct: 122 TTGGLRGRFYTVTDASDADVVNPKPGTLRHAVVQKRPLWIIFGRSMIIRLSKELLVTSDK 181

Query: 181 TIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDG 240
           TIDARGANVHIA+GAGISIQF +NVIIHGLHIHHI+ A+GG+IRD+ +HVGLRT SDGDG
Sbjct: 182 TIDARGANVHIAYGAGISIQFTRNVIIHGLHIHHILSASGGMIRDADDHVGLRTVSDGDG 241

Query: 241 VSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSV 300
           +SIFGS+N+WLDHLSMSNC DGLIDAIQGSTAIT+SNSHFTHHNDVMLFGASDS+QGDS+
Sbjct: 242 ISIFGSSNVWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSFQGDSI 301

Query: 301 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 360
           MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP
Sbjct: 302 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 361

Query: 361 NPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGT 420
           NPAAKQITNRNYATE++WKTWTWRSEGDL+MNGAYFV+SG+PSKRRPYSR DMIKA+PGT
Sbjct: 362 NPAAKQITNRNYATESIWKTWTWRSEGDLLMNGAYFVESGDPSKRRPYSRFDMIKARPGT 421

Query: 421 FVRRLTRFSGSLNCFVGRPC 437
           FVRRLTRFSGSLNC+VGRPC
Sbjct: 422 FVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of MC08g1370 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 567.8 bits (1462), Expect = 7.7e-162
Identity = 271/468 (57.91%), Postives = 341/468 (72.86%), Query Frame = 0


Query: 5   RVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQ-- 64
           R+  LM    +AV++PT+R  I+E DEY  Q+A+EA+  +L+A+HSDP  + DHF+ +  
Sbjct: 5   RLSKLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHY 64

Query: 65  ------------------------VHLALEGSNSTRRSMK--------KYNGPCMATNPI 124
                                   + +    +NSTRRS++        K  GPC A+NPI
Sbjct: 65  DNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPI 124

Query: 125 DRCWRCDPNWANNRKKLATCVLGFGRKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAV 184
           D+CWRC  +WA  RKKL  CV GFG +T GG +G+IYVVT   D D+VNPKPGTLR+  +
Sbjct: 125 DKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVI 184

Query: 185 QKKPLWIIFGRSMIITLSKELLVASHKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIH 244
           QK+PLWIIF   M I L++ELL+ SHKTIDARGANVH+A GAGI++QF KNV+IHGLHIH
Sbjct: 185 QKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIH 244

Query: 245 HIIVATGGLIRDSTNHVGLRTNSDGDGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAI 304
           HI  ++GG+IRDS +H G+RT +DGDG+SI+GS+NIWLDH+SMS C DGLIDAI GST I
Sbjct: 245 HISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGI 304

Query: 305 TISNSHFTHHNDVMLFGASDSYQGDSVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDY 364
           TISNSHFTHHNDVML GA ++ + D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDY
Sbjct: 305 TISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDY 364

Query: 365 THWLMYAIGGSQHPTIISQGNRFIAPPN-PAAKQITNRNYATEAVWKTWTWRSEGDLMMN 424
           THW +YAIGGSQ PTI+S GNRFIAPP+ P  +++T R+YA+E  WK W WRS+ D+ MN
Sbjct: 365 THWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMN 424

Query: 425 GAYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           GAYF QSGNP  +  ++R  MIK K G  V +LT+++G+L+C VGR C
Sbjct: 425 GAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of MC08g1370 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 558.5 bits (1438), Expect = 4.7e-159
Identity = 267/467 (57.17%), Postives = 336/467 (71.95%), Query Frame = 0


Query: 9   LMLLVSVAVILPTLRAGIA-EYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQ----- 68
           L+ ++ +A ++PT+RA +A E DEY + KA EA+  +L A+H DP  + DHF+ +     
Sbjct: 9   LVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNS 68

Query: 69  -------------------VHLALEGSNSTRRSM------------KKYNGPCMATNPID 128
                              + +    +NSTRRS+             K  GPC A+NPID
Sbjct: 69  TDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPID 128

Query: 129 RCWRCDPNWANNRKKLATCVLGFGRKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQ 188
           +CWRC P+WA  RKKL  CV GFG +T GG +G+IYVVT P D D+VNP+PGTLR+  +Q
Sbjct: 129 KCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQ 188

Query: 189 KKPLWIIFGRSMIITLSKELLVASHKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIHH 248
           K+PLWI+F   M I LS+EL++ S KTIDARGANVHIA+GAGI++Q+  N+IIHGLH+HH
Sbjct: 189 KEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHH 248

Query: 249 IIVATGGLIRDSTNHVGLRTNSDGDGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAIT 308
           I+ ++GGLIRDS NH G R  +DGDG+SIFG+TNIWLDH+SMS C DGLIDAI GSTAIT
Sbjct: 249 IVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAIT 308

Query: 309 ISNSHFTHHNDVMLFGASDSYQGDSVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYT 368
           ISNSHFTHHNDVML GA ++   D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDYT
Sbjct: 309 ISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYT 368

Query: 369 HWLMYAIGGSQHPTIISQGNRFIAPPNPA-AKQITNRNYATEAVWKTWTWRSEGDLMMNG 428
           HW +YAIGGSQ PTI+S GNRFIAPP+    +++T R+YA+E+ WK W WRSE D+ MN 
Sbjct: 369 HWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNN 428

Query: 429 AYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           AYF QSGNP  +  +SR  MIK K G  V +LT+++G+L+C VG+ C
Sbjct: 429 AYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of MC08g1370 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 537.0 bits (1382), Expect = 1.5e-152
Identity = 256/447 (57.27%), Postives = 318/447 (71.14%), Query Frame = 0


Query: 8   FLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVHLAL 67
           F+     +A++ P +R  +A +D Y  Q+  +A   ++ ++   P+NVT+HFN  V++A+
Sbjct: 13  FVFFSSFLAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAV 72

Query: 68  EGS---NSTRRSM---------KKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFG 127
           + S   N TRR +          K +G C+A NPID CWRCD NWANNRKKLA CVLGFG
Sbjct: 73  DASESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFG 132

Query: 128 RKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVAS 187
           R+T GG  G IYVV D SD D++NPKPGTLR+   +  PLWIIF RSMII L +EL++ S
Sbjct: 133 RRTTGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITS 192

Query: 188 HKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDG 247
            KTID RGA V+I  GAG+++QF  NVIIH +++ HI+   GGLIRDS  H+GLRT SDG
Sbjct: 193 DKTIDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDG 252

Query: 248 DGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGD 307
           DG+S+FG+TNIW+DH+SM+ CADG+IDAI GSTA+TISNSHFT H +VMLFGA D +  D
Sbjct: 253 DGISLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVID 312

Query: 308 SVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIA 367
             MQ+TVAFNHFGK L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIA
Sbjct: 313 KKMQITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIA 372

Query: 368 PPNPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSG-----NPSKRRPYSRHDM 427
           PPN  AKQIT R Y     WK+W W+SEGD  +NGAYFVQSG     +   + P      
Sbjct: 373 PPNEEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFT 432

Query: 428 IKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           I+ KPGT VR+LT  +G L C +G  C
Sbjct: 433 IRPKPGTMVRKLTMDAGVLGCKLGEAC 459

[Close]

BLAST of MC08g1370 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 511.1 bits (1315), Expect = 8.5e-145
Identity = 249/447 (55.70%), Postives = 319/447 (71.36%), Query Frame = 0


Query: 8   FLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVHLAL 67
           F+ + +S+ V++P+++A IAEYDEY  Q+   A   +LE++  +P NVTDHFN    LA+
Sbjct: 9   FVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAM 68

Query: 68  EGS---NSTRRSMK---------KYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFG 127
           E +   N TRR ++         +  G   + N ID+CWR D NW  NRKKLA CVLGFG
Sbjct: 69  ETTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFG 128

Query: 128 RKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVAS 187
           RKT GG  G IYVVTDPSD D++ PKPGT+R+   + +PLWIIF RSMII L +EL++ +
Sbjct: 129 RKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITN 188

Query: 188 HKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDG 247
            KTID RGA ++I  GAG+++QF +NVIIH +HI  I    GGLI DS  H GLRT SDG
Sbjct: 189 DKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDG 248

Query: 248 DGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGD 307
           DG++IFG+TN+W+DH+SM++C+DG+IDAI GSTAITISNSHFT H++VMLFG ++    D
Sbjct: 249 DGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVID 308

Query: 308 SVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIA 367
             MQ+TVAFNHFGK L QRMPR R+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIA
Sbjct: 309 KKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIA 368

Query: 368 PPNPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSG-----NPSKRRPYSRHDM 427
           PP   +KQ+T R Y     WK+W W+SE D  +NGAYFVQSG     + + + P  R   
Sbjct: 369 PPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFA 428

Query: 428 IKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           I+ +PGT VRRLT+ +G+L C  G+ C
Sbjct: 429 IRPQPGTKVRRLTKDAGTLGCKPGKSC 455

[Close]

BLAST of MC08g1370 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 469.2 bits (1206), Expect = 3.7e-132
Identity = 225/391 (57.54%), Postives = 280/391 (71.61%), Query Frame = 0


Query: 47  AFHSDPMNVTDHFNLQVHLALEGSNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKK 106
           A   DP  V D     V ++ E + + RR +  ++  C   NPID CWRCD NW  NRK+
Sbjct: 84  AVADDPDMVADEVAKLVQMS-EQNRTARRKLGFFS--CGTGNPIDDCWRCDRNWHKNRKR 143

Query: 107 LATCVLGFGRKTRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIIT 166
           LA C +GFGR   GG  G+ Y+VTDP+D DVVNPKPGTLR+  +Q++PLWI+F R M+I 
Sbjct: 144 LADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIE 203

Query: 167 LSKELLVASHKTIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNH 226
           L +EL++ S KTIDARG+NVHIA GA I+IQF  NVIIHGLHIH        ++R S +H
Sbjct: 204 LKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSH 263

Query: 227 VGLRTNSDGDGVSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLF 286
            G RT +DGD VSIFGS++IW+DH S+S+CADGL+DA+ GSTAIT+SN+HFTHHN+VML 
Sbjct: 264 FGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLL 323

Query: 287 GASDSYQGDSVMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTI 346
           G SDSY  D +MQVT+A+NHFG+GLVQRMPRCR G+FHVVNNDYTHW MYAIGGS  PTI
Sbjct: 324 GHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTI 383

Query: 347 ISQGNRFIAPPNPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYS 406
            SQGNR+ AP +  AK++T R     + WK W WRSEGDL++NGA+F  SG       Y 
Sbjct: 384 NSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG-AGASASYG 443

Query: 407 RHDMIKAKPGTFVRRLTRFSGSLNCFVGRPC 438
           R   + AKP + V  +T  +G+L C  GRPC
Sbjct: 444 RASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

The following BLAST results are available for this feature:

BLAST of MC08g1370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LFP5	8.3e-161	58.12	Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1	[more]
P40973	4.5e-159	63.61	Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1	[more]
Q9SRH4	6.6e-158	57.17	Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1	[more]
P15722	2.3e-155	59.46	Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1	[more]
Q9M9S2	2.0e-151	57.27	Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1	[more]

BLAST of MC08g1370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152137.1	0.0	100.00	probable pectate lyase 19 [Momordica charantia]	[more]
XP_004147462.2	3.24e-295	86.82	probable pectate lyase 7 [Cucumis sativus] >KGN59593.1 hypothetical protein Csa_...	[more]
XP_008443432.1	1.87e-294	87.24	PREDICTED: probable pectate lyase 19 [Cucumis melo]	[more]
XP_038905811.1	2.43e-290	85.75	pectate lyase-like [Benincasa hispida]	[more]
KAG7017718.1	5.83e-282	82.50	putative pectate lyase P59 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC08g1370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DFD2	0.0	100.00	Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111019925 PE=3 SV=1	[more]
A0A0A0LCU3	1.57e-295	86.82	Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G827350 PE=3 SV=1	[more]
A0A1S3B8R7	9.06e-295	87.24	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487022 PE=3 SV=1	[more]
A0A6J1F1Q5	8.08e-282	82.50	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111441594 PE=3 SV=1	[more]
A0A6J1J8N7	1.15e-281	82.05	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111482295 PE=3 SV=1	[more]

BLAST of MC08g1370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15110.1	7.7e-162	57.91	Pectate lyase family protein	[more]
AT3G01270.1	4.7e-159	57.17	Pectate lyase family protein	[more]
AT1G14420.1	1.5e-152	57.27	Pectate lyase family protein	[more]
AT2G02720.1	8.5e-145	55.70	Pectate lyase family protein	[more]
AT4G13710.1	3.7e-132	57.54	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 170..186 score: 64.99 coord: 378..402 score: 53.71 coord: 408..431 score: 42.46 coord: 234..255 score: 57.58 coord: 134..159 score: 65.75 coord: 336..355 score: 79.52 coord: 110..127 score: 55.29 coord: 314..333 score: 78.1
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 162..359 e-value: 5.1E-98 score: 341.6
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 173..353 e-value: 8.5E-19 score: 68.0
IPR007524	Pectate lyase, N-terminal	PFAM	PF04431	Pec_lyase_N	coord: 26..76 e-value: 5.4E-19 score: 68.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 87..430 e-value: 5.1E-112 score: 376.2
None	No IPR available	PANTHER	PTHR31683:SF146	PECTATE LYASE	coord: 6..437
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 6..437
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 88..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g1370.1	MC08g1370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity