MC08g1305 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1305
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionphotosystem I assembly factor PSA3, chloroplastic
LocationMC08: 11647450 .. 11650441 (-)
RNA-Seq ExpressionMC08g1305
SyntenyMC08g1305
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCCTGTCCTCTACTTCCACTTCATCTTCTTTCCGCCCAAATCTCAATTCCATTTCCATTCCCAAGCCCAGAACCTGCTTCCTCTGTAATCCGATTCCGACTTCTCCGGCGATTCTCCTCCTCCGCCGCCGCCGAGCCTTGTCCGTGAGATCGTACATGGAGAATCCGAATTCTCTGTCCGGATTCGCGAACAAGGTCATCGGTTCTCTCCCGGTGGTCGGCCTGATCGCCAGGATTCTCAGCGACGAAGGCGGCGTCGGCACCGACATTATCGATTTCGCCGAGTTTCGGAGACGAGTTGGGAAGAAATGTACGGTCATGGATTCTAGGGCTTTCTACGAATTTCAAAACAGAAGAGGCAAGGTTAGTGTGTAACTGTATATATATATGTAGAGATTTTTAATTAATTACTTTCTTTAGCAAATCACTTTTTTTTTTAAATGTTATGGTGTTTAATTGAAACCTCTTCTTTCTACTGTGGAAGAATTATTAATTTATTTGGAGTTTAGAGTGTATTTATTATAGTTTTTTCTAAGTGGTTTTAAATAGTTAACACTGATTGGATAAAAATTTTCTATTATTAGTTTTTTCCAATACTATTTTAATATTTGGTTTCTATCCTTTTTAACTGGGGTGAACTATTTAAAATTATTTCTCAAACTTTAAGAATTAAAAGGTGTTCGTTTTGCAACTTCATGGACCATGTAAACATCAAATTCAAGCTCGAGTCAAAAGTCTACATTTCACTATTTCAGACCAAATAGACAGTATCTATATTTTTACGGTGAGATTGATATCTTGATTGATCCCAATTTTTAAAATTTGAGTTAAAAAAATCAGCTACACAAGCCCTCCTGTCAGGGAAAAAAAAAACCCCTAAGGCGAACCCTTTTCTAAGTAGAGTTAATGGTGGAAGTTAAAAAGATTTAAAATCAAATCTTATGAAGTCTTAAATTTTCATTTGGTCTCGTTTTTTTTATTATTATTATTTATTTTAATCTAGGTACTTTTTTCGTTTCGGTCCCTATACTTAAAAAGAAAATATCAAGTCTCTTTATTTTATTTTTTATCTCATTTTTTAGTCACAATTTCAAACACTACTTTTAATATATTTCTTAAAATGTAGTGATTTCTAGCTTTTTTGTTGAATCTGTGTTAAAATTTTGCAATCAAAAACCAAAATGGACCACTTTGAAAGTACAGAGACAAAAACAGACGTTTAGAAAGTACAATAATTAAAATGAAAAGTCGAATGTATAAGGACTAAAATGGAACCAAAATAAATGTTTTGAAAATATTTTGATCAAACAGCACCAAAATAATATGTTGTTTTAATTCAATCATAAGATTTTGAAGTGAAGTTTTGGTAATTGTAGGCAGGAGAGCCATTGTATGTTCTATTGTGCTGTTGGCTGGCAGCTGTGGGAGCTGGTTTGCTTAAAACAGAGGAGATTTTGGAGGGAGTGGCAAGGCTCAGGATTTCAGATGATATTGAATTTGAAGAGGAGACTTTCCTTTCCATGATGAATGAGGCTAGAGAGGTTGGCTAATCTTGCTCTTACATCATCTTTTTTTATATTTGTTTGGAATTCTATTTTTCATCCGAAATTTTTACATTTATTTTTAGTTAAACCACACGTTTAGTCTCTGAATTTTTAATATATAATTAATGTCTGAACTTTAAAAAGTATCTAATGCGTTGTGTTAATGGGTTTGTAGATTTTTTTAAAAAATGTCTGATAGGTCAGGACTATTCAACAAGAAAAAAAAGAAGAAGAAAGCTGAGAGACCTATTAGACGAGATTGAAAGTGATGAAAGTTCTTAGACTAAACTTGTAATTCAACCTTTGTCTTATTTTAGTTTTTGAACTTTTAAAATATATATTTTAATCTCAAAAAATGATCATTTTGATCGTCATCATTGTCATTGCTCTACCGTCAACTTTCTCATTGTCTTTTCCTTATTGTTGGTCTATAATCAACCCTAATTAGTATTTTAATACATATTTGAATCTACGTTATTCGTTTGTTAAACAATCGATAGCAGTAATAATCGAAATTATTTAATTATTTATTTTTAAAAATTCACGGACTAAAGTGAATATTTCAAAAGTTCACGATCAGAATAGAATGAACATGTAATTCAACGATCAAAATGAGATTTAAACTTTTGCATTCTTCTAATGAAAGCTATAAGCACCGTAGTCATCTTACACCAACTAACAATATACATTCTTCACGATTGTCGTTCACTTCCAGAGACGAGGAAAAGTAAAAGCTGCTACTCCAAACATCCCGATGCAGGTCCGAGTCGAGAAAGCTCTCGACGCTATCTATGTCTGTTCGTTCGGGAGAGATCCGATAGAGGAAGACGATGAGAAACTGATAGTAATTATGCTAAGTGCTGTGTTTCCATCGGTTGGGCAAACTGAGATTCAAAGGATTGTGAAAGAGAAGGCAGTTAGAATTGCTGAAGGCAAAGACAGTGCCATTGTTCCTGAGCCGAAGCCTTTGTCAAAAGAAGCCATTTTGCTGCAAATGAAGGACCTCCAATTCCTCCAAGAGAAGAGTGAGACTTAATAATGATGATTATATTGAGTTGCATAATTTGTAAGAATTGAATTTTCTGCTGCACGGTTCTCTTGTCTAGAAAATATTGCCTGTTAATTACGATATCAATATGGTTCTAGATTAGAGTAGTGAGTTCGGGTAAAATCGAGTTGATATAATTTGCTCATAAAGCTAAAATGTTCGATTAAATTATGTTATGGTCAAGGTTTTAAATGTCGATGTCTTAATTTTATGGAAATGTCGATAAAAATATTGATGTTGGATAAATATTTTTTAAAAAATCTTTAAATCAATAAATAAGTCTTTCATTTGTTATAGGTAGAATCAGAGTCTGCCACATGGTCCTTTCATCTAAAAAAATAATGTTTGGACCTCACACTAAACAAGCTAAGACCAAGGAAGGCCAGACACGATTCAT

mRNA sequence

ATGTCCCTGTCCTCTACTTCCACTTCATCTTCTTTCCGCCCAAATCTCAATTCCATTTCCATTCCCAAGCCCAGAACCTGCTTCCTCTGTAATCCGATTCCGACTTCTCCGGCGATTCTCCTCCTCCGCCGCCGCCGAGCCTTGTCCGTGAGATCGTACATGGAGAATCCGAATTCTCTGTCCGGATTCGCGAACAAGGTCATCGGTTCTCTCCCGGTGGTCGGCCTGATCGCCAGGATTCTCAGCGACGAAGGCGGCGTCGGCACCGACATTATCGATTTCGCCGAGTTTCGGAGACGAGTTGGGAAGAAATGTACGGTCATGGATTCTAGGGCTTTCTACGAATTTCAAAACAGAAGAGGCAAGGTTAGAGAGCCATTGTATGTTCTATTGTGCTGTTGGCTGGCAGCTGTGGGAGCTGGTTTGCTTAAAACAGAGGAGATTTTGGAGGGAGTGGCAAGGCTCAGGATTTCAGATGATATTGAATTTGAAGAGGAGACTTTCCTTTCCATGATGAATGAGGCTAGAGAGAGACGAGGAAAAGTAAAAGCTGCTACTCCAAACATCCCGATGCAGGTCCGAGTCGAGAAAGCTCTCGACGCTATCTATGTCTGTTCGTTCGGGAGAGATCCGATAGAGGAAGACGATGAGAAACTGATAGTAATTATGCTAAGTGCTGTGTTTCCATCGGTTGGGCAAACTGAGATTCAAAGGATTGTGAAAGAGAAGGCAGTTAGAATTGCTGAAGGCAAAGACAGTGCCATTGTTCCTGAGCCGAAGCCTTTGTCAAAAGAAGCCATTTTGCTGCAAATGAAGGACCTCCAATTCCTCCAAGAGAAGAGTGAGACTTAATAATGATGATTATATTGAGTTGCATAATTTGTAAGAATTGAATTTTCTGCTGCACGGTTCTCTTGTCTAGAAAATATTGCCTGTTAATTACGATATCAATATGGTTCTAGATTAGAGTAGTGAGTTCGGGTAAAATCGAGTTGATATAATTTGCTCATAAAGCTAAAATGTTCGATTAAATTATGTTATGGTCAAGGTTTTAAATGTCGATGTCTTAATTTTATGGAAATGTCGATAAAAATATTGATGTTGGATAAATATTTTTTAAAAAATCTTTAAATCAATAAATAAGTCTTTCATTTGTTATAGGTAGAATCAGAGTCTGCCACATGGTCCTTTCATCTAAAAAAATAATGTTTGGACCTCACACTAAACAAGCTAAGACCAAGGAAGGCCAGACACGATTCAT

Coding sequence (CDS)

ATGTCCCTGTCCTCTACTTCCACTTCATCTTCTTTCCGCCCAAATCTCAATTCCATTTCCATTCCCAAGCCCAGAACCTGCTTCCTCTGTAATCCGATTCCGACTTCTCCGGCGATTCTCCTCCTCCGCCGCCGCCGAGCCTTGTCCGTGAGATCGTACATGGAGAATCCGAATTCTCTGTCCGGATTCGCGAACAAGGTCATCGGTTCTCTCCCGGTGGTCGGCCTGATCGCCAGGATTCTCAGCGACGAAGGCGGCGTCGGCACCGACATTATCGATTTCGCCGAGTTTCGGAGACGAGTTGGGAAGAAATGTACGGTCATGGATTCTAGGGCTTTCTACGAATTTCAAAACAGAAGAGGCAAGGTTAGAGAGCCATTGTATGTTCTATTGTGCTGTTGGCTGGCAGCTGTGGGAGCTGGTTTGCTTAAAACAGAGGAGATTTTGGAGGGAGTGGCAAGGCTCAGGATTTCAGATGATATTGAATTTGAAGAGGAGACTTTCCTTTCCATGATGAATGAGGCTAGAGAGAGACGAGGAAAAGTAAAAGCTGCTACTCCAAACATCCCGATGCAGGTCCGAGTCGAGAAAGCTCTCGACGCTATCTATGTCTGTTCGTTCGGGAGAGATCCGATAGAGGAAGACGATGAGAAACTGATAGTAATTATGCTAAGTGCTGTGTTTCCATCGGTTGGGCAAACTGAGATTCAAAGGATTGTGAAAGAGAAGGCAGTTAGAATTGCTGAAGGCAAAGACAGTGCCATTGTTCCTGAGCCGAAGCCTTTGTCAAAAGAAGCCATTTTGCTGCAAATGAAGGACCTCCAATTCCTCCAAGAGAAGAGTGAGACTTAA

Protein sequence

MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET
Homology
BLAST of MC08g1305 vs. ExPASy Swiss-Prot
Match: Q9M3C6 (Photosystem I assembly factor PSA3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSA3 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.2e-85
Identity = 163/253 (64.43%), Postives = 201/253 (79.45%), Query Frame = 0

Query: 29  LCNPIPTSPAILLLRRRRALSVRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG 88
           L NP  TS +   L    ALS+R+Y+E PNS S FANKVIGSLPV+GL+ARI+SDEGGVG
Sbjct: 23  LRNPSTTSSSRPKLDGGFALSIRAYIEKPNSFSTFANKVIGSLPVIGLLARIISDEGGVG 82

Query: 89  TDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEI 148
            D++DFAEFR+RVG KCT  DSRAFYEFQ RRGK  EPLYVLLCCW+AAVGAGLLK+EEI
Sbjct: 83  RDLVDFAEFRKRVGNKCTPDDSRAFYEFQQRRGKAGEPLYVLLCCWVAAVGAGLLKSEEI 142

Query: 149 LEGVARLRISDDIEFEEETFLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFG 208
           LEGV R+ IS+D+EFEE+ F+++M EAR+RR K+    P IPM++RVEKAL+ IY C F 
Sbjct: 143 LEGVTRVSISNDLEFEEQNFIALMTEARQRRAKLNIDAPTIPMELRVEKALEGIYACCFR 202

Query: 209 RDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAIL 268
           R  IEE+DEKL+ +ML AVFPSV ++EI+RI+KEKA R+ EG +   V   K L KEAI 
Sbjct: 203 RGLIEEEDEKLLKVMLIAVFPSVEKSEIERIIKEKATRVEEGGEEENV-MAKRLPKEAIQ 262

Query: 269 LQMKDLQFLQEKS 282
           +QMKDL+FLQ+++
Sbjct: 263 MQMKDLEFLQQQN 274

BLAST of MC08g1305 vs. ExPASy Swiss-Prot
Match: B4FSG1 (Photosystem I assembly factor PSA3, chloroplastic OS=Zea mays OX=4577 GN=PSA3 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 7.9e-80
Identity = 149/238 (62.61%), Postives = 191/238 (80.25%), Query Frame = 0

Query: 43  RRRRALSVRSYME-NPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRV 102
           RRRR  +V +YME NPNS +  A +++G+LP+VGL+ARIL+DEGGVG DIIDFAEFRRRV
Sbjct: 29  RRRRYRAVVAYMEPNPNSPAAIAGRLVGALPIVGLVARILNDEGGVGGDIIDFAEFRRRV 88

Query: 103 GKKCTVMDSRAFYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDI 162
            KKCTVMDS+AFY+F  RRGK  +P YVLLCCWLAA+GAGLLKTEEILEGVARLRIS+DI
Sbjct: 89  SKKCTVMDSKAFYDFNQRRGKPGDPFYVLLCCWLAAIGAGLLKTEEILEGVARLRISNDI 148

Query: 163 EFEEETFLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIV 222
           EFEEETF+ MM  A+E+R K+KA  P IPM+ R EKAL+AIYVC FG+D +E++DEKL+ 
Sbjct: 149 EFEEETFIDMMRAAKEKRAKLKAPAPQIPMETRAEKALEAIYVCCFGQDMVEDEDEKLLR 208

Query: 223 IMLSAVFPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQE 280
            +L+AVFPSVG+  ++R+V   A ++A G+      + + +SKE    Q+KDL+FL++
Sbjct: 209 AILNAVFPSVGRAAVERMVASMAKQVASGERKR---DGRTVSKEVQQRQLKDLEFLKQ 263

BLAST of MC08g1305 vs. NCBI nr
Match: XP_022158919.1 (photosystem I assembly factor PSA3, chloroplastic [Momordica charantia])

HSP 1 Score: 537 bits (1384), Expect = 4.09e-192
Identity = 281/283 (99.29%), Postives = 281/283 (99.29%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSL 60
           MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSL
Sbjct: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSL 60

Query: 61  SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR 120
           SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR
Sbjct: 61  SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR 120

Query: 121 GKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG 180
           GK  EPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG
Sbjct: 121 GKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG 180

Query: 181 KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV 240
           KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV
Sbjct: 181 KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV 240

Query: 241 KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET
Sbjct: 241 KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283

BLAST of MC08g1305 vs. NCBI nr
Match: XP_004141087.1 (photosystem I assembly factor PSA3, chloroplastic [Cucumis sativus] >KGN59665.1 hypothetical protein Csa_000816 [Cucumis sativus])

HSP 1 Score: 425 bits (1093), Expect = 9.08e-148
Identity = 228/291 (78.35%), Postives = 257/291 (88.32%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S     P+  FL NPI  SP  L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKFFFLSNPISPSPLFLRKLYGRRRNPNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YME+PNSLSGFA+KVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMESPNSLSGFASKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GK  EPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETFL+MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFLAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VR+EKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRIEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           + EIQRI+KEKA+RIAEGKD+AIVPEP+PLSKEAIL QMKDLQFLQE +ET
Sbjct: 241 EIEIQRILKEKAIRIAEGKDTAIVPEPQPLSKEAILAQMKDLQFLQENNET 284

BLAST of MC08g1305 vs. NCBI nr
Match: XP_008443515.1 (PREDICTED: uncharacterized protein LOC103487086 [Cucumis melo] >KAA0053674.1 putative ubiquinone biosynthesis UbiB [Cucumis melo var. makuwa] >TYK14001.1 putative ubiquinone biosynthesis UbiB [Cucumis melo var. makuwa])

HSP 1 Score: 423 bits (1088), Expect = 5.24e-147
Identity = 227/291 (78.01%), Postives = 257/291 (88.32%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S     P++ FL NPI  S   L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKSFFLSNPISPSRLFLPQLYGRRRSSNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YMENPNSLSGFA+KVIGSLPVVGLIARI+SDEGGVG+D+IDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMENPNSLSGFASKVIGSLPVVGLIARIMSDEGGVGSDLIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GK  EPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETF++MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFIAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           + EIQRIVKEKA+RIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQEK+ET
Sbjct: 241 EIEIQRIVKEKAIRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQEKNET 284

BLAST of MC08g1305 vs. NCBI nr
Match: XP_038905081.1 (photosystem I assembly factor PSA3, chloroplastic [Benincasa hispida])

HSP 1 Score: 420 bits (1079), Expect = 1.23e-145
Identity = 232/294 (78.91%), Postives = 253/294 (86.05%), Query Frame = 0

Query: 1   MSLSSTS-TSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLR----RRR------ALS 60
           MS++STS TSSS   NL S         FL  PI  SP+ L LR    RRR       LS
Sbjct: 1   MSVTSTSSTSSSLHSNLKSF--------FLSKPI--SPSRLFLRQIYGRRRQSNSAGVLS 60

Query: 61  VRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMD 120
           V++YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG+DIIDFAEFRRRVGKKCT+M+
Sbjct: 61  VKAYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGSDIIDFAEFRRRVGKKCTIME 120

Query: 121 SRAFYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFL 180
           S+AFYEFQ R+GK  EPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETFL
Sbjct: 121 SKAFYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFL 180

Query: 181 SMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFP 240
           +MMNEARE+R K+K A PNIPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVF 
Sbjct: 181 AMMNEAREKREKLKVAVPNIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFA 240

Query: 241 SVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           SV +TEIQRIVKEKA+RIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQEK+ET
Sbjct: 241 SVRETEIQRIVKEKAIRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQEKTET 284

BLAST of MC08g1305 vs. NCBI nr
Match: KAG7022731.1 (hypothetical protein SDJN02_16466 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 419 bits (1078), Expect = 1.87e-145
Identity = 230/296 (77.70%), Postives = 254/296 (85.81%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLR-------RRR------A 60
           M+++STST+SSF  N        P++ FL + I  SP  LL R       RRR       
Sbjct: 1   MAVTSTSTTSSFHSN--------PKSFFLSSLI--SPPKLLHRQFHGSVGRRRHPTSTGV 60

Query: 61  LSVRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTV 120
           LSVR+YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG+DIIDFAEFRRRVGKKCT+
Sbjct: 61  LSVRAYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGSDIIDFAEFRRRVGKKCTI 120

Query: 121 MDSRAFYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEET 180
           MDS+AFY+FQ RRGK  EPL+VLLCCWLAAVGAGLLK+EEILEGVARLRISDDIEFEEET
Sbjct: 121 MDSKAFYDFQERRGKPGEPLFVLLCCWLAAVGAGLLKSEEILEGVARLRISDDIEFEEET 180

Query: 181 FLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAV 240
           FL+MMNEARE+R K+K A P IPM+VRVEKALDAIYVCSFGRDPIEE+DEKL+VIMLS+V
Sbjct: 181 FLAMMNEAREKREKLKVAVPKIPMEVRVEKALDAIYVCSFGRDPIEEEDEKLLVIMLSSV 240

Query: 241 FPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           FP+VGQ+EIQRIVKEKAVRIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQE SET
Sbjct: 241 FPTVGQSEIQRIVKEKAVRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQENSET 286

BLAST of MC08g1305 vs. ExPASy TrEMBL
Match: A0A6J1E0U9 (photosystem I assembly factor PSA3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111025378 PE=4 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 1.98e-192
Identity = 281/283 (99.29%), Postives = 281/283 (99.29%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSL 60
           MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSL
Sbjct: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSL 60

Query: 61  SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR 120
           SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR
Sbjct: 61  SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR 120

Query: 121 GKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG 180
           GK  EPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG
Sbjct: 121 GKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG 180

Query: 181 KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV 240
           KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV
Sbjct: 181 KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV 240

Query: 241 KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET
Sbjct: 241 KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283

BLAST of MC08g1305 vs. ExPASy TrEMBL
Match: A0A0A0LCC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G836490 PE=4 SV=1)

HSP 1 Score: 425 bits (1093), Expect = 4.40e-148
Identity = 228/291 (78.35%), Postives = 257/291 (88.32%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S     P+  FL NPI  SP  L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKFFFLSNPISPSPLFLRKLYGRRRNPNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YME+PNSLSGFA+KVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMESPNSLSGFASKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GK  EPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETFL+MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFLAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VR+EKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRIEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           + EIQRI+KEKA+RIAEGKD+AIVPEP+PLSKEAIL QMKDLQFLQE +ET
Sbjct: 241 EIEIQRILKEKAIRIAEGKDTAIVPEPQPLSKEAILAQMKDLQFLQENNET 284

BLAST of MC08g1305 vs. ExPASy TrEMBL
Match: A0A5D3CQC9 (Putative ubiquinone biosynthesis UbiB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119G00270 PE=4 SV=1)

HSP 1 Score: 423 bits (1088), Expect = 2.54e-147
Identity = 227/291 (78.01%), Postives = 257/291 (88.32%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S     P++ FL NPI  S   L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKSFFLSNPISPSRLFLPQLYGRRRSSNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YMENPNSLSGFA+KVIGSLPVVGLIARI+SDEGGVG+D+IDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMENPNSLSGFASKVIGSLPVVGLIARIMSDEGGVGSDLIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GK  EPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETF++MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFIAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           + EIQRIVKEKA+RIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQEK+ET
Sbjct: 241 EIEIQRIVKEKAIRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQEKNET 284

BLAST of MC08g1305 vs. ExPASy TrEMBL
Match: A0A1S3B876 (uncharacterized protein LOC103487086 OS=Cucumis melo OX=3656 GN=LOC103487086 PE=4 SV=1)

HSP 1 Score: 423 bits (1088), Expect = 2.54e-147
Identity = 227/291 (78.01%), Postives = 257/291 (88.32%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S     P++ FL NPI  S   L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKSFFLSNPISPSRLFLPQLYGRRRSSNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YMENPNSLSGFA+KVIGSLPVVGLIARI+SDEGGVG+D+IDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMENPNSLSGFASKVIGSLPVVGLIARIMSDEGGVGSDLIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GK  EPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETF++MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFIAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           + EIQRIVKEKA+RIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQEK+ET
Sbjct: 241 EIEIQRIVKEKAIRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQEKNET 284

BLAST of MC08g1305 vs. ExPASy TrEMBL
Match: A0A6J1EQR9 (photosystem I assembly factor PSA3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435039 PE=4 SV=1)

HSP 1 Score: 419 bits (1077), Expect = 1.29e-145
Identity = 230/296 (77.70%), Postives = 254/296 (85.81%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLR-------RRR------A 60
           M+++STST+SSF  N        P++ FL + I  SP  LL R       RRR       
Sbjct: 1   MAVTSTSTTSSFHSN--------PKSFFLYSLI--SPPKLLHRQFHGSVGRRRHPTSTGV 60

Query: 61  LSVRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTV 120
           LSVR+YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG+DIIDFAEFRRRVGKKCT+
Sbjct: 61  LSVRAYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGSDIIDFAEFRRRVGKKCTI 120

Query: 121 MDSRAFYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEET 180
           MDS+AFY+FQ RRGK  EPL+VLLCCWLAAVGAGLLK+EEILEGVARLRISDDIEFEEET
Sbjct: 121 MDSKAFYDFQERRGKPGEPLFVLLCCWLAAVGAGLLKSEEILEGVARLRISDDIEFEEET 180

Query: 181 FLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAV 240
           FL+MMNEARE+R K+K A P IPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLS+V
Sbjct: 181 FLAMMNEAREKREKLKVAVPKIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSSV 240

Query: 241 FPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283
           FP+VGQ+EIQRIVKEKAVRIAEGKD AIVPEP+PLSKEAIL QMKDLQFL+E SET
Sbjct: 241 FPTVGQSEIQRIVKEKAVRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLRENSET 286

BLAST of MC08g1305 vs. TAIR 10
Match: AT3G55250.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion, chloroplast, nucleus; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; Has 46 Blast hits to 46 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 317.0 bits (811), Expect = 1.5e-86
Identity = 163/253 (64.43%), Postives = 201/253 (79.45%), Query Frame = 0

Query: 29  LCNPIPTSPAILLLRRRRALSVRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG 88
           L NP  TS +   L    ALS+R+Y+E PNS S FANKVIGSLPV+GL+ARI+SDEGGVG
Sbjct: 23  LRNPSTTSSSRPKLDGGFALSIRAYIEKPNSFSTFANKVIGSLPVIGLLARIISDEGGVG 82

Query: 89  TDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRRGKVREPLYVLLCCWLAAVGAGLLKTEEI 148
            D++DFAEFR+RVG KCT  DSRAFYEFQ RRGK  EPLYVLLCCW+AAVGAGLLK+EEI
Sbjct: 83  RDLVDFAEFRKRVGNKCTPDDSRAFYEFQQRRGKAGEPLYVLLCCWVAAVGAGLLKSEEI 142

Query: 149 LEGVARLRISDDIEFEEETFLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFG 208
           LEGV R+ IS+D+EFEE+ F+++M EAR+RR K+    P IPM++RVEKAL+ IY C F 
Sbjct: 143 LEGVTRVSISNDLEFEEQNFIALMTEARQRRAKLNIDAPTIPMELRVEKALEGIYACCFR 202

Query: 209 RDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAIL 268
           R  IEE+DEKL+ +ML AVFPSV ++EI+RI+KEKA R+ EG +   V   K L KEAI 
Sbjct: 203 RGLIEEEDEKLLKVMLIAVFPSVEKSEIERIIKEKATRVEEGGEEENV-MAKRLPKEAIQ 262

Query: 269 LQMKDLQFLQEKS 282
           +QMKDL+FLQ+++
Sbjct: 263 MQMKDLEFLQQQN 274

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3C62.2e-8564.43Photosystem I assembly factor PSA3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
B4FSG17.9e-8062.61Photosystem I assembly factor PSA3, chloroplastic OS=Zea mays OX=4577 GN=PSA3 PE... [more]
Match NameE-valueIdentityDescription
XP_022158919.14.09e-19299.29photosystem I assembly factor PSA3, chloroplastic [Momordica charantia][more]
XP_004141087.19.08e-14878.35photosystem I assembly factor PSA3, chloroplastic [Cucumis sativus] >KGN59665.1 ... [more]
XP_008443515.15.24e-14778.01PREDICTED: uncharacterized protein LOC103487086 [Cucumis melo] >KAA0053674.1 put... [more]
XP_038905081.11.23e-14578.91photosystem I assembly factor PSA3, chloroplastic [Benincasa hispida][more]
KAG7022731.11.87e-14577.70hypothetical protein SDJN02_16466 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1E0U91.98e-19299.29photosystem I assembly factor PSA3, chloroplastic OS=Momordica charantia OX=3673... [more]
A0A0A0LCC24.40e-14878.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G836490 PE=4 SV=1[more]
A0A5D3CQC92.54e-14778.01Putative ubiquinone biosynthesis UbiB OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3B8762.54e-14778.01uncharacterized protein LOC103487086 OS=Cucumis melo OX=3656 GN=LOC103487086 PE=... [more]
A0A6J1EQR91.29e-14577.70photosystem I assembly factor PSA3, chloroplastic OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT3G55250.11.5e-8664.43unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037736Photosystem I assembly factor PSA3PANTHERPTHR36770PHOTOSYSTEM I ASSEMBLY FACTOR PSA3, CHLOROPLASTICcoord: 43..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1305.1MC08g1305.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048564 photosystem I assembly
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0098572 stromal side of plastid thylakoid membrane