MC08g1304 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1304
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionFormate dehydrogenase, mitochondrial
LocationMC08: 11641707 .. 11646775 (+)
RNA-Seq ExpressionMC08g1304
SyntenyMC08g1304
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TACAGAAGTGGAAAGTATTTCTTAACCCACGTGAACAAAATATGAGAGAAAAAGTAGAATCAAAACACTGTAAAAAAGAAAAAAGCAATATTTGAATATTGAATGAATAAAAGCAAAGAAAGAGAACACCGACGTGGCGGCGTGCAGAACAAAGGCAGTCTCCGGAGAGAACAGAACTGAAAAAGAAAGCAAAGAGCGCTTCGTCTTCCAATGGGACTTCGCCAACTATAAATACAGCGCTGCGAACCGTGAAACTCGTAATTCATCACCATCATTCACAATAAAGCTTTTTCAGAGAGAATCAGAAGGCGACAATGGCGATGAAGCAGGCAGCTACCACGGCAGTTCGAGCTCTTTCTTCCTCTGCAACCTCGCAATCATCGTCTCTGGTCCGCCATCTCCATGTGAGCCCCTGTTCTCTCTTTGATGCTAAACCAATTTGATTTTCATGTTCATTACGAAGTTCGAACTTCTTCTTCTTCCTCTTCCTCTGTGGAGGAGTAGCAGAAGTTGCGGATTTCTCCTTAATCCCTCGCATGCGGACTCCTCTTCTCTCGTTCTTAGATTCTTGATTTTTCCCCTATTCTTGGTTAATTAAGATTGATTGCTACAATAGGAAACCTACTTATTCGTATTTCTGTGTGATTTATGAGTTTGATTGGGGAGCTGGAGGAAGGATTATGGATTATTGAAGTTTCTTTTGAGATCTGTTAGTTTTTTTTAGTCTCCGATCATTATTAAGATGGATTGCTACAACAGGAGTCCTAATTTGTCTCTGTGTGATATCGAGTTTGATTGGGAGCTGGATGAAGGATTAATGGATAACTGAAATTGCTTTTTGTGATTTGTAATCTGCTCGAGTCCCCGATCGTAAAGATAGATTCATAGAACTGGAAAGCCTACTTTATGTCTCTGTTTGATTGTGAGCTGGAGGGAGGATTTATGGATAATTGGAGTTGCTGTCGTGATTTGTGAATATCATCTTAAGTGAATTAATTAATGGCTTTCGTTTTGAGCAGGCTTCTCCAGGGAGCAAGAAGATCGTCGGAGTGTTTTACAAGGCGAATGAGTATGCTGCTCTGAACCCTAATTTCGTGGGATGTGTGGAGGGAGCCTTGGGCATTCGCGAGTGGTTGGAATCGCAAGGTCATCAGTATATTGTTACCGATGACAAGGAAGGACTCGATTCTGGTACGCATTAGACAAATACTTACCCGATGTGCATGAATTTTCTACTTTGGAAATGGCCTGTGATCTTGGTTAGATTCAAAGAACATTCGGGGCCAGAGTAGGAAAATTCTCGTTTGCAAGCTGAAATGAGATAAAACGAAGACATTAAAATATTTTAGATATAGAAGTTTTTAATTGGGCTTCGTATTCTAAAGATAATATTTTAAATTTGAACTTTTCATTTCAACTTTAGTAGTCAATATTTTAGATATGTTTTCACTTTAATTTTCTTTTTACTAAAAAAGCATTACATGATTGCTAGTTGGACTTTGTCACTCAACTGTTCAATTTCAAATAATTGAAGTGTAATACCTTTTATGGAGTCCGCGTCAGGGGCTGCCGTTTTCAACTATCTGCAGATTCTTCTCAAAATTCAAATTCTCTTGGGTCATATTATTTAGCTCAACATGTATATATCTTTATGACAACTTAGTGTAGAAGATCTTCACTTAGACTTGTTTTCTTTCTAAATTTAGGGTTCACCTGATTTGTTTGCTTTCTAAATTTAGTCCTCTCTCCCTTCCATCTGAAATGAGAGCGTAATCAAAAATCAGAAAATTCCTTCTCCGAATATTTTTCTTCTCTATACTTATTTGACAAGATGTTTCCCAAATAAAACTAACAAAATTGAATGTAATTTCTTTTTTTTACGAGTACCACAACTCTGGAGTGGGATTTGAACTTTAAAATGAATATATATTTCAATTATGCTCATGTCAGAAAATTGTATAGACTAAAAAATATCCAATTTTTGGAGTATGCTAAACAATCAATTTAGTGCCTGATAATATATTTGTTCTTAATAGAGTTATTTGACCACTAAGAGACGTTAGGTAAGCCTTAATATGGAAAATCTTGATAATATGATCAAAAGTCAAAATTTAAGGCCTCGTTTTATAATAGATTGGTTTTTAGTTTTCTATTTTTAAAGATTAGATTCCTCTCAATTTTTTTACTTCTTTTAACAAATTTGAATCCTTATCCAAATTTTAAAAATAAAAACAAGTTTTAAAAAAATAGTTTTCAAAATTTGGCATACATTTTAAAAATGTTTTTAGAAAGTAGATAATAGAGAAAAGTAGTGTTTACATGCATTCAAAAAAATAAAATAGTTTTAAACGGGACCTAAATCATTACCATTCTAAACACATAACTAGAAATTAACTTAAATTAATGAACATTACCATGATTTAAGTTATAAATATATTTTTTGGCATATTACTTATTTTAACATGACAGTATCAAATGATCTATGCATGATATCAAATTAGTTTAGAACATAAAAATATTTTAAATCCCTACAAAATATATATTGCAGGTGATTAATCACATATTATCTGTGTTCTGATTGAAGTCATGTGGTTTTCTGCATCCTTGTTTCAGAGCTTGAAAAGCATATTCCAGATCTCCATGTTCTCATAAGCACCCCCTTCCATCCTGCCTATGTAACTGCTGAAAGGATCAAAAAGGCCAAAAACTTGCAGTTGCTTCTCACTGCTGGAATTGGATCTGATCATGTTGACCTCAATGCTGCTGCTGCTGCTGGACTAACAGTTGCAGAGGTTACTGGAAGCAATGTGGTTTCAGTTGCAGAGGACGAGCTAATGAGAATTCTCATTCTTGTCAGGAATTTCCTACCAGGACACCACCAGGTTATTAATGGCGAATGGAATGTCGCTGGAATTGCTCATAGGGCTTACGATCTCGAGGGGAAGACAGTTGGGACCGTAGGCGCTGGGCGAATCGGCAAACTCTTACTTCAAAGGTTGAAACCTTTCAACTGCAATCTCCTCTACCACGACCGACTCAAGATCGATCCTGAACTTGAGAAGCAGATAGGAGCGCAGTTTGAAGAGGACCTGGATGCAATGCTACCAAAATGTGATGTACTTGTCATCAACACTCCTCTAACTGACAAGACAAGGTATTGCTTCAACTTTAGACTGTAAATTGAGATGATAAACATAAGAAAAACTTTGAATAATACTCTAAATTGGAAGCTTGGATTCTTCATACTAATGGAAATTGATTCTGCTTCGCTGCTGCAGGGGACTATTTAACAAAGAGAGAATTGCCAAGTGCAAGAAGGGAGTGCTGATTGTCAACAATGCTCGAGGAGCAATCATGGACACCCAAGCAGTTGTTGACGCTTGTAACAGCGGACATATTGGAGGTAATTTAAGAATAAAATTCCATACTAATGTGTCTGGATATAACACCTTGATCTGTCACGTACATCGGAAAGCTGAAGTGGCGCTATCGCCATGTCTTGTCAGCTTCCTACATGTAATCATATAACATATCAAGGTGTTCATTTAGCTTTTGTTGGCGATATTTTACTTTCTAGTATTATATAATCAGATAATTGACATTAAGAGACTAAATTGATATGTTTGCAAAAGTTTGGTGTGGAAGTATGAGATCAAAACATCACAAGTAGCAGAATATAGAGACTGAAATTGTAACCATTCAATAAAATCATTATGAGTTACTATGGATCCATTAAGTGAAGTAAAAGAAACTCGAACCAATTGCATTGTTGGCTAATACTTCAAAGAATTCTGAATATGCAGGTTACAGTGGTGATGTGTGGAACCCACAACCAGCTCCAAAAGACCATCCATGGCGTTACATGCCAAACCAAGCTATGACACCTCATATTTCTGGTACCACCATTGATGCTCAGGTATAGTTTCAAAATAAGAGCAATTGTTTTAAATCTAGAAGAATGTATATAAAATAGGCTAATAAATTATACCAAAATGGCTTTAGATTATGTAGCTGGTTTAAAACGTATATTAGGGGCAAAAAATAAAAGAAATGAATTACATAGAATGCTCCTAAAGTGGTTTTTAGTTTCAATTTTACCCCAATCTTATAAAACAAATTAAAAGATGAAGGGTAAAATTGAAACGTCTAAAGCGGTAGGGGTAAAATTGAGACCATGATCGGCCAAAGCAAGCATAGTTCAGTGGTAATTGACATATATCTCCAACCAAGAGGTTGTGAGCTCGATCCCCCACCCCCACATGTTGTACTCGGAAAAAAAAATTGAGACCATAACCAGCCAAGAACGTTTTATATGCTTTTTCCACTGATCTTGAAAGAGGTATTTTATTTTATATATAAAAAGTTAGAAGGTAAAATTTTATCTGAGTTCATGCATTGGATGGCAGTTGCGATATGCTGCGGGAGTGAAGGACATGCTTGAGAGGTATTTCAAAGGAGAAGAATTTCCCGCTCAGAACTACATCGTGAAGGAAGGAGAACTCGCACCTCAGTACCGATAAATAGTTCTCTCTCTGATGCTTCTCTGGCTTTGGCTTGGAATTTGGATTTAGCTCCCTCCCCCTTTTGCCATTTTAGTCAAAAATAAAAATAGTTTCCAAAATAAAAGTTGGCTTCCATGGGATCGGAGTTGCTTAACTTGTTTCCTTAAATCAGTTTCTTGTGAGTCTTTGTTGGTAATCAGACGTAATGTAATGTAATTCCATGTAAAATGGAATGTATGAGGATGAATGAGAGCTTCTTACTTTTCCATCCAAAAGTTGTATTCATCGAATTGAATGAATTCCCAATGAAAAAGATTGAAAACCAACAAAATCGACCAAGATTTAACTCATTTGGTAGAAAACTGGTAAGGATTCTCCATTGAAAAGAAAAATGTTCTTACGAAGGTATATAGTAAATATGAGCTACTTCTCTTATGGCCAATAAATTTTGGGATGAGATCTATGAATATAGTATGAGCCTAAAGAACTATGATTTTGAGAGGATGTATTAAAAATTCAACGTTGAAAAGATTTCATCTCGACTTGTAGTATTGATAAAACAACAAG

mRNA sequence

TACAGAAGTGGAAAGTATTTCTTAACCCACGTGAACAAAATATGAGAGAAAAAGTAGAATCAAAACACTGTAAAAAAGAAAAAAGCAATATTTGAATATTGAATGAATAAAAGCAAAGAAAGAGAACACCGACGTGGCGGCGTGCAGAACAAAGGCAGTCTCCGGAGAGAACAGAACTGAAAAAGAAAGCAAAGAGCGCTTCGTCTTCCAATGGGACTTCGCCAACTATAAATACAGCGCTGCGAACCGTGAAACTCGTAATTCATCACCATCATTCACAATAAAGCTTTTTCAGAGAGAATCAGAAGGCGACAATGGCGATGAAGCAGGCAGCTACCACGGCAGTTCGAGCTCTTTCTTCCTCTGCAACCTCGCAATCATCGTCTCTGGTCCGCCATCTCCATGCTTCTCCAGGGAGCAAGAAGATCGTCGGAGTGTTTTACAAGGCGAATGAGTATGCTGCTCTGAACCCTAATTTCGTGGGATGTGTGGAGGGAGCCTTGGGCATTCGCGAGTGGTTGGAATCGCAAGGTCATCAGTATATTGTTACCGATGACAAGGAAGGACTCGATTCTGAGCTTGAAAAGCATATTCCAGATCTCCATGTTCTCATAAGCACCCCCTTCCATCCTGCCTATGTAACTGCTGAAAGGATCAAAAAGGCCAAAAACTTGCAGTTGCTTCTCACTGCTGGAATTGGATCTGATCATGTTGACCTCAATGCTGCTGCTGCTGCTGGACTAACAGTTGCAGAGGTTACTGGAAGCAATGTGGTTTCAGTTGCAGAGGACGAGCTAATGAGAATTCTCATTCTTGTCAGGAATTTCCTACCAGGACACCACCAGGTTATTAATGGCGAATGGAATGTCGCTGGAATTGCTCATAGGGCTTACGATCTCGAGGGGAAGACAGTTGGGACCGTAGGCGCTGGGCGAATCGGCAAACTCTTACTTCAAAGGTTGAAACCTTTCAACTGCAATCTCCTCTACCACGACCGACTCAAGATCGATCCTGAACTTGAGAAGCAGATAGGAGCGCAGTTTGAAGAGGACCTGGATGCAATGCTACCAAAATGTGATGTACTTGTCATCAACACTCCTCTAACTGACAAGACAAGGGGACTATTTAACAAAGAGAGAATTGCCAAGTGCAAGAAGGGAGTGCTGATTGTCAACAATGCTCGAGGAGCAATCATGGACACCCAAGCAGTTGTTGACGCTTGTAACAGCGGACATATTGGAGGTTACAGTGGTGATGTGTGGAACCCACAACCAGCTCCAAAAGACCATCCATGGCGTTACATGCCAAACCAAGCTATGACACCTCATATTTCTGGTACCACCATTGATGCTCAGTTGCGATATGCTGCGGGAGTGAAGGACATGCTTGAGAGGTATTTCAAAGGAGAAGAATTTCCCGCTCAGAACTACATCGTGAAGGAAGGAGAACTCGCACCTCAGTACCGATAAATAGTTCTCTCTCTGATGCTTCTCTGGCTTTGGCTTGGAATTTGGATTTAGCTCCCTCCCCCTTTTGCCATTTTAGTCAAAAATAAAAATAGTTTCCAAAATAAAAGTTGGCTTCCATGGGATCGGAGTTGCTTAACTTGTTTCCTTAAATCAGTTTCTTGTGAGTCTTTGTTGGTAATCAGACGTAATGTAATGTAATTCCATGTAAAATGGAATGTATGAGGATGAATGAGAGCTTCTTACTTTTCCATCCAAAAGTTGTATTCATCGAATTGAATGAATTCCCAATGAAAAAGATTGAAAACCAACAAAATCGACCAAGATTTAACTCATTTGGTAGAAAACTGGTAAGGATTCTCCATTGAAAAGAAAAATGTTCTTACGAAGGTATATAGTAAATATGAGCTACTTCTCTTATGGCCAATAAATTTTGGGATGAGATCTATGAATATAGTATGAGCCTAAAGAACTATGATTTTGAGAGGATGTATTAAAAATTCAACGTTGAAAAGATTTCATCTCGACTTGTAGTATTGATAAAACAACAAG

Coding sequence (CDS)

ATGGCGATGAAGCAGGCAGCTACCACGGCAGTTCGAGCTCTTTCTTCCTCTGCAACCTCGCAATCATCGTCTCTGGTCCGCCATCTCCATGCTTCTCCAGGGAGCAAGAAGATCGTCGGAGTGTTTTACAAGGCGAATGAGTATGCTGCTCTGAACCCTAATTTCGTGGGATGTGTGGAGGGAGCCTTGGGCATTCGCGAGTGGTTGGAATCGCAAGGTCATCAGTATATTGTTACCGATGACAAGGAAGGACTCGATTCTGAGCTTGAAAAGCATATTCCAGATCTCCATGTTCTCATAAGCACCCCCTTCCATCCTGCCTATGTAACTGCTGAAAGGATCAAAAAGGCCAAAAACTTGCAGTTGCTTCTCACTGCTGGAATTGGATCTGATCATGTTGACCTCAATGCTGCTGCTGCTGCTGGACTAACAGTTGCAGAGGTTACTGGAAGCAATGTGGTTTCAGTTGCAGAGGACGAGCTAATGAGAATTCTCATTCTTGTCAGGAATTTCCTACCAGGACACCACCAGGTTATTAATGGCGAATGGAATGTCGCTGGAATTGCTCATAGGGCTTACGATCTCGAGGGGAAGACAGTTGGGACCGTAGGCGCTGGGCGAATCGGCAAACTCTTACTTCAAAGGTTGAAACCTTTCAACTGCAATCTCCTCTACCACGACCGACTCAAGATCGATCCTGAACTTGAGAAGCAGATAGGAGCGCAGTTTGAAGAGGACCTGGATGCAATGCTACCAAAATGTGATGTACTTGTCATCAACACTCCTCTAACTGACAAGACAAGGGGACTATTTAACAAAGAGAGAATTGCCAAGTGCAAGAAGGGAGTGCTGATTGTCAACAATGCTCGAGGAGCAATCATGGACACCCAAGCAGTTGTTGACGCTTGTAACAGCGGACATATTGGAGGTTACAGTGGTGATGTGTGGAACCCACAACCAGCTCCAAAAGACCATCCATGGCGTTACATGCCAAACCAAGCTATGACACCTCATATTTCTGGTACCACCATTGATGCTCAGTTGCGATATGCTGCGGGAGTGAAGGACATGCTTGAGAGGTATTTCAAAGGAGAAGAATTTCCCGCTCAGAACTACATCGTGAAGGAAGGAGAACTCGCACCTCAGTACCGATAA

Protein sequence

MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVEGALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNLQLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVINGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIGAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVVDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLERYFKGEEFPAQNYIVKEGELAPQYR
Homology
BLAST of MC08g1304 vs. ExPASy Swiss-Prot
Match: Q07511 (Formate dehydrogenase, mitochondrial OS=Solanum tuberosum OX=4113 GN=FDH1 PE=1 SV=2)

HSP 1 Score: 674.1 bits (1738), Expect = 9.4e-193
Identity = 332/384 (86.46%), Postives = 353/384 (91.93%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAM + A+TA RA++S +   S    R L ASPG KKIVGVFYKANEYA +NPNF+GC E
Sbjct: 1   MAMSRVASTAARAITSPS---SLVFTRELQASPGPKKIVGVFYKANEYAEMNPNFLGCAE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
            ALGIREWLES+GHQYIVT DKEG D ELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL
Sbjct: 61  NALGIREWLESKGHQYIVTPDKEGPDCELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDL AAAAAGLTVAEVTGSN VSVAEDELMRILILVRNFLPGHHQVIN
Sbjct: 121 QLLLTAGIGSDHVDLKAAAAAGLTVAEVTGSNTVSVAEDELMRILILVRNFLPGHHQVIN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVA IAHRAYDLEGKTVGTVGAGRIG+LLLQRLKPFNCNLLYHDRLK+D ELE QIG
Sbjct: 181 GEWNVAAIAHRAYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLLYHDRLKMDSELENQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEEDLD ML KCD++VINTPLT+KT+G+F+KERIAK KKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AKFEEDLDKMLSKCDIVVINTPLTEKTKGMFDKERIAKLKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHI GYSGDVW PQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAG KDML+R
Sbjct: 301 DACNSGHIAGYSGDVWYPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGTKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKGE+FPA+NYIVK+GELAPQYR
Sbjct: 361 YFKGEDFPAENYIVKDGELAPQYR 381

BLAST of MC08g1304 vs. ExPASy Swiss-Prot
Match: Q9S7E4 (Formate dehydrogenase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FDH1 PE=1 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 4.7e-184
Identity = 320/385 (83.12%), Postives = 350/385 (90.91%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPG-SKKIVGVFYKANEYAALNPNFVGCV 60
           MAM+QAA   +RA SSS++S   +  R  +AS G SKKIVGVFYKANEYA  NPNF+GCV
Sbjct: 1   MAMRQAAKATIRACSSSSSSGYFAR-RQFNASSGDSKKIVGVFYKANEYATKNPNFLGCV 60

Query: 61  EGALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKN 120
           E ALGIR+WLESQGHQYIVTDDKEG D ELEKHIPDLHVLISTPFHPAYVTAERIKKAKN
Sbjct: 61  ENALGIRDWLESQGHQYIVTDDKEGPDCELEKHIPDLHVLISTPFHPAYVTAERIKKAKN 120

Query: 121 LQLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 180
           L+LLLTAGIGSDH+DL AAAAAGLTVAEVTGSNVVSVAEDELMRILIL+RNF+PG++QV+
Sbjct: 121 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 180

Query: 181 NGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQI 240
            GEWNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPF CNLLYHDRL++ PELEK+ 
Sbjct: 181 KGEWNVAGIAYRAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHDRLQMAPELEKET 240

Query: 241 GAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAV 300
           GA+F EDL+ MLPKCDV+VIN PLT+KTRG+FNKE I K KKGVLIVNNARGAIM+ QAV
Sbjct: 241 GAKFVEDLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGVLIVNNARGAIMERQAV 300

Query: 301 VDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLE 360
           VDA  SGHIGGYSGDVW+PQPAPKDHPWRYMPNQAMTPH SGTTIDAQLRYAAG KDMLE
Sbjct: 301 VDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQAMTPHTSGTTIDAQLRYAAGTKDMLE 360

Query: 361 RYFKGEEFPAQNYIVKEGELAPQYR 385
           RYFKGE+FP +NYIVK+GELAPQYR
Sbjct: 361 RYFKGEDFPTENYIVKDGELAPQYR 384

BLAST of MC08g1304 vs. ExPASy Swiss-Prot
Match: Q9SXP2 (Formate dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0486800 PE=1 SV=2)

HSP 1 Score: 643.3 bits (1658), Expect = 1.8e-183
Identity = 319/386 (82.64%), Postives = 347/386 (89.90%), Query Frame = 0

Query: 1   MAMKQAATTAV--RALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGC 60
           MAM +AA   +  RAL S A           H S GSKKIVGVFYK  EYA  NPNFVGC
Sbjct: 1   MAMWRAAAGHLLGRALGSRAA----------HTSAGSKKIVGVFYKGGEYADKNPNFVGC 60

Query: 61  VEGALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAK 120
           VEGALGIREWLES+GH YIVTDDKEGL+SELEKHI D+HVLI+TPFHPAYV+AERIKKAK
Sbjct: 61  VEGALGIREWLESKGHHYIVTDDKEGLNSELEKHIEDMHVLITTPFHPAYVSAERIKKAK 120

Query: 121 NLQLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQV 180
           NL+LLLTAGIGSDH+DL AAAAAGLTVAEVTGSN VSVAEDELMRILIL+RNFLPG+ QV
Sbjct: 121 NLELLLTAGIGSDHIDLPAAAAAGLTVAEVTGSNTVSVAEDELMRILILLRNFLPGYQQV 180

Query: 181 INGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQ 240
           ++GEWNVAGIA+RAYDLEGKTVGTVGAGRIG+LLLQRLKPFNCNLLYHDRLKIDPELEK+
Sbjct: 181 VHGEWNVAGIAYRAYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLLYHDRLKIDPELEKE 240

Query: 241 IGAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQA 300
           IGA++EEDLDAMLPKCDV+VINTPLT+KTRG+FNKERIAK KKGV+IVNNARGAIMDTQA
Sbjct: 241 IGAKYEEDLDAMLPKCDVIVINTPLTEKTRGMFNKERIAKMKKGVIIVNNARGAIMDTQA 300

Query: 301 VVDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDML 360
           V DAC+SG + GY GDVW PQPAPKDHPWRYMPN AMTPHISGTTIDAQLRYAAGVKDML
Sbjct: 301 VADACSSGQVAGYGGDVWFPQPAPKDHPWRYMPNHAMTPHISGTTIDAQLRYAAGVKDML 360

Query: 361 ERYFKGEEFPAQNYIVKEGELAPQYR 385
           +RYFKGE+FP QNYIVKEG+LA QY+
Sbjct: 361 DRYFKGEDFPVQNYIVKEGQLASQYQ 376

BLAST of MC08g1304 vs. ExPASy Swiss-Prot
Match: Q9ZRI8 (Formate dehydrogenase, mitochondrial OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 2.6e-182
Identity = 306/358 (85.47%), Postives = 332/358 (92.74%), Query Frame = 0

Query: 27  RHLHASPGSKKIVGVFYKANEYAALNPNFVGCVEGALGIREWLESQGHQYIVTDDKEGLD 86
           R  H S GSKKIVGVFY+A EYA  NPNFVGCVEGALGIR+WLES+GH YIVTDDKEG +
Sbjct: 20  RAAHTSAGSKKIVGVFYQAGEYADKNPNFVGCVEGALGIRDWLESKGHHYIVTDDKEGFN 79

Query: 87  SELEKHIPDLHVLISTPFHPAYVTAERIKKAKNLQLLLTAGIGSDHVDLNAAAAAGLTVA 146
           SELEKHI D+HVLI+TPFHPAYVTAE+IKKAK  +LLLTAGIGSDH+DL AAAAAGLTVA
Sbjct: 80  SELEKHIEDMHVLITTPFHPAYVTAEKIKKAKTPELLLTAGIGSDHIDLPAAAAAGLTVA 139

Query: 147 EVTGSNVVSVAEDELMRILILVRNFLPGHHQVINGEWNVAGIAHRAYDLEGKTVGTVGAG 206
            VTGSN VSVAEDELMRILIL+RNFLPG+ QV+ GEWNVAGIAHRAYDLEGKTVGTVGAG
Sbjct: 140 RVTGSNTVSVAEDELMRILILLRNFLPGYQQVVKGEWNVAGIAHRAYDLEGKTVGTVGAG 199

Query: 207 RIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIGAQFEEDLDAMLPKCDVLVINTPLTDK 266
           R G+LLLQRLKPFNCNLLYHDRL+I+PELEK+IGA+FEEDLDAMLPKCDV+VINTPLT+K
Sbjct: 200 RYGRLLLQRLKPFNCNLLYHDRLQINPELEKEIGAKFEEDLDAMLPKCDVVVINTPLTEK 259

Query: 267 TRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVVDACNSGHIGGYSGDVWNPQPAPKDHP 326
           TRG+FNKE+IAK KKGV+IVNNARGAIMDTQAV DAC+SGHI GY GDVW PQPAPKDHP
Sbjct: 260 TRGMFNKEKIAKMKKGVIIVNNARGAIMDTQAVADACSSGHIAGYGGDVWFPQPAPKDHP 319

Query: 327 WRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLERYFKGEEFPAQNYIVKEGELAPQYR 385
           WRYMPN AMTPHISGTTIDAQLRYAAGVKDML+RYFKGEEFP +NYIVKEGELA QY+
Sbjct: 320 WRYMPNHAMTPHISGTTIDAQLRYAAGVKDMLDRYFKGEEFPVENYIVKEGELASQYK 377

BLAST of MC08g1304 vs. ExPASy Swiss-Prot
Match: Q67U69 (Formate dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0486900 PE=2 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 2.7e-179
Identity = 303/384 (78.91%), Postives = 340/384 (88.54%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAM +A + A + L  +  S ++        S GSKK+VGVFYK  EYA  NPNFVGCV+
Sbjct: 1   MAMWRAPSAAGQLLGRALASTAA------QTSAGSKKVVGVFYKGGEYADKNPNFVGCVD 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
            ALGIR WLES+GH+YIVTDDKEG++ ELEKHI D HVLI+TPFHPAY+TAERIKKAKNL
Sbjct: 61  SALGIRGWLESKGHRYIVTDDKEGINCELEKHIEDAHVLITTPFHPAYITAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           +LLLTAG+GSDH+DL AAAAAGLTVAE+TGSN VSVAED+LMRIL+L+RNFLPGHHQ++N
Sbjct: 121 ELLLTAGVGSDHIDLPAAAAAGLTVAEITGSNTVSVAEDQLMRILLLLRNFLPGHHQIVN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHR YDLEGKTVGTVGAGRIG+LLLQRLKPFNCNL+YHDR+KIDPELEK+IG
Sbjct: 181 GEWNVAGIAHRTYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLMYHDRVKIDPELEKEIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A++EEDLDAMLPKCDV+VIN PLT+KTRG+FNKERIAK KKGV IVNNARGAIMDTQAV 
Sbjct: 241 AKYEEDLDAMLPKCDVVVINMPLTEKTRGMFNKERIAKMKKGVTIVNNARGAIMDTQAVA 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DAC SGH+ GY GDVW PQPAPKDHPWRYMPN AMTPH SGTTID QLRYAAGVKDML+R
Sbjct: 301 DACASGHVAGYGGDVWFPQPAPKDHPWRYMPNHAMTPHCSGTTIDGQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKGE+FPAQNYIVK G+LA QY+
Sbjct: 361 YFKGEDFPAQNYIVKAGQLASQYQ 378

BLAST of MC08g1304 vs. NCBI nr
Match: XP_022158921.1 (formate dehydrogenase, mitochondrial [Momordica charantia])

HSP 1 Score: 764 bits (1973), Expect = 1.74e-278
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE
Sbjct: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKGEEFPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGELAPQYR 384

BLAST of MC08g1304 vs. NCBI nr
Match: XP_038904948.1 (formate dehydrogenase, mitochondrial [Benincasa hispida])

HSP 1 Score: 742 bits (1916), Expect = 8.50e-270
Identity = 372/384 (96.88%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAAT A+RALSSS TS SS L+R+LHAS GSKKIVGVFYKANEYAALNPNFVGCVE
Sbjct: 1   MAMKQAATAAIRALSSSVTSHSSPLLRNLHASAGSKKIVGVFYKANEYAALNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGHQYIVTDDKEGLD ELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKTVGTVGAGRIG+LLLQRLKPFNCNLLYHDRLKID ELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLLYHDRLKIDSELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKGEEFPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGELAPQYR 384

BLAST of MC08g1304 vs. NCBI nr
Match: XP_004141089.1 (formate dehydrogenase, mitochondrial [Cucumis sativus] >KGN59666.1 hypothetical protein Csa_002069 [Cucumis sativus])

HSP 1 Score: 742 bits (1915), Expect = 1.21e-269
Identity = 369/384 (96.09%), Postives = 379/384 (98.70%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAATTA+RALSSS TS SS L+RHLHAS  SKKIVGVFYKANEYAA+NPNFVGCVE
Sbjct: 1   MAMKQAATTAIRALSSSLTSHSSPLLRHLHASAESKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGH+YIVTDDKEGLDSELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHEYIVTDDKEGLDSELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           +LLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPG+HQV+N
Sbjct: 121 KLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGYHQVVN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGH+GGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER
Sbjct: 301 DACNSGHVGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKGEEFPAQNYIVKEG+LAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGQLAPQYR 384

BLAST of MC08g1304 vs. NCBI nr
Match: XP_008443517.1 (PREDICTED: formate dehydrogenase, mitochondrial [Cucumis melo] >KAA0053673.1 formate dehydrogenase [Cucumis melo var. makuwa] >TYK14002.1 formate dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 739 bits (1907), Expect = 2.00e-268
Identity = 368/384 (95.83%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAATTA+RALSSS  S SS LVR LHAS  SKKIVGVFYKANEYAA+NPNFVGCVE
Sbjct: 1   MAMKQAATTAIRALSSSLISHSSPLVRQLHASAESKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGH+YIVTDDKEGLDSELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHEYIVTDDKEGLDSELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPG+HQV+N
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGYHQVVN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAG+KDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGIKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKGEEFPAQNYIVKEG+LAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGQLAPQYR 384

BLAST of MC08g1304 vs. NCBI nr
Match: XP_022934681.1 (formate dehydrogenase, mitochondrial isoform X3 [Cucurbita moschata])

HSP 1 Score: 736 bits (1901), Expect = 1.64e-267
Identity = 365/384 (95.05%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAAT A+RA SSS TSQSS LVRHLHAS GSKKIVGVFYKANEYAA+NPNFVGCVE
Sbjct: 1   MAMKQAATAAIRAFSSSVTSQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+ ELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDT+AVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTEAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKG+ FPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGQNFPAQNYIVKEGELAPQYR 384

BLAST of MC08g1304 vs. ExPASy TrEMBL
Match: A0A6J1E2C8 (Formate dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111025380 PE=3 SV=1)

HSP 1 Score: 764 bits (1973), Expect = 8.43e-279
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE
Sbjct: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKGEEFPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGELAPQYR 384

BLAST of MC08g1304 vs. ExPASy TrEMBL
Match: A0A0A0LHY6 (Formate dehydrogenase, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_3G836500 PE=3 SV=1)

HSP 1 Score: 742 bits (1915), Expect = 5.85e-270
Identity = 369/384 (96.09%), Postives = 379/384 (98.70%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAATTA+RALSSS TS SS L+RHLHAS  SKKIVGVFYKANEYAA+NPNFVGCVE
Sbjct: 1   MAMKQAATTAIRALSSSLTSHSSPLLRHLHASAESKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGH+YIVTDDKEGLDSELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHEYIVTDDKEGLDSELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           +LLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPG+HQV+N
Sbjct: 121 KLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGYHQVVN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGH+GGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER
Sbjct: 301 DACNSGHVGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKGEEFPAQNYIVKEG+LAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGQLAPQYR 384

BLAST of MC08g1304 vs. ExPASy TrEMBL
Match: A0A5D3CRJ9 (Formate dehydrogenase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119G00280 PE=3 SV=1)

HSP 1 Score: 739 bits (1907), Expect = 9.69e-269
Identity = 368/384 (95.83%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAATTA+RALSSS  S SS LVR LHAS  SKKIVGVFYKANEYAA+NPNFVGCVE
Sbjct: 1   MAMKQAATTAIRALSSSLISHSSPLVRQLHASAESKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGH+YIVTDDKEGLDSELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHEYIVTDDKEGLDSELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPG+HQV+N
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGYHQVVN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAG+KDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGIKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKGEEFPAQNYIVKEG+LAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGQLAPQYR 384

BLAST of MC08g1304 vs. ExPASy TrEMBL
Match: A0A1S3B8Z5 (Formate dehydrogenase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103487087 PE=3 SV=1)

HSP 1 Score: 739 bits (1907), Expect = 9.69e-269
Identity = 368/384 (95.83%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAATTA+RALSSS  S SS LVR LHAS  SKKIVGVFYKANEYAA+NPNFVGCVE
Sbjct: 1   MAMKQAATTAIRALSSSLISHSSPLVRQLHASAESKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGH+YIVTDDKEGLDSELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHEYIVTDDKEGLDSELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPG+HQV+N
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGYHQVVN 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAG+KDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGIKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKGEEFPAQNYIVKEG+LAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGQLAPQYR 384

BLAST of MC08g1304 vs. ExPASy TrEMBL
Match: A0A6J1F2G8 (Formate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111441799 PE=3 SV=1)

HSP 1 Score: 736 bits (1901), Expect = 7.96e-268
Identity = 365/384 (95.05%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60
           MAMKQAAT A+RA SSS TSQSS LVRHLHAS GSKKIVGVFYKANEYAA+NPNFVGCVE
Sbjct: 1   MAMKQAATAAIRAFSSSVTSQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+ ELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180
           QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240
           GEWNVAGIAHRAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDT+AVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTEAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 384
           YFKG+ FPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGQNFPAQNYIVKEGELAPQYR 384

BLAST of MC08g1304 vs. TAIR 10
Match: AT5G14780.1 (formate dehydrogenase )

HSP 1 Score: 645.2 bits (1663), Expect = 3.3e-185
Identity = 320/385 (83.12%), Postives = 350/385 (90.91%), Query Frame = 0

Query: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPG-SKKIVGVFYKANEYAALNPNFVGCV 60
           MAM+QAA   +RA SSS++S   +  R  +AS G SKKIVGVFYKANEYA  NPNF+GCV
Sbjct: 1   MAMRQAAKATIRACSSSSSSGYFAR-RQFNASSGDSKKIVGVFYKANEYATKNPNFLGCV 60

Query: 61  EGALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKN 120
           E ALGIR+WLESQGHQYIVTDDKEG D ELEKHIPDLHVLISTPFHPAYVTAERIKKAKN
Sbjct: 61  ENALGIRDWLESQGHQYIVTDDKEGPDCELEKHIPDLHVLISTPFHPAYVTAERIKKAKN 120

Query: 121 LQLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 180
           L+LLLTAGIGSDH+DL AAAAAGLTVAEVTGSNVVSVAEDELMRILIL+RNF+PG++QV+
Sbjct: 121 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 180

Query: 181 NGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQI 240
            GEWNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPF CNLLYHDRL++ PELEK+ 
Sbjct: 181 KGEWNVAGIAYRAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHDRLQMAPELEKET 240

Query: 241 GAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAV 300
           GA+F EDL+ MLPKCDV+VIN PLT+KTRG+FNKE I K KKGVLIVNNARGAIM+ QAV
Sbjct: 241 GAKFVEDLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGVLIVNNARGAIMERQAV 300

Query: 301 VDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLE 360
           VDA  SGHIGGYSGDVW+PQPAPKDHPWRYMPNQAMTPH SGTTIDAQLRYAAG KDMLE
Sbjct: 301 VDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQAMTPHTSGTTIDAQLRYAAGTKDMLE 360

Query: 361 RYFKGEEFPAQNYIVKEGELAPQYR 385
           RYFKGE+FP +NYIVK+GELAPQYR
Sbjct: 361 RYFKGEDFPTENYIVKDGELAPQYR 384

BLAST of MC08g1304 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 117.1 bits (292), Expect = 3.2e-26
Identity = 76/258 (29.46%), Postives = 127/258 (49.22%), Query Frame = 0

Query: 109 VTAERIKKAK-NLQLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILIL 168
           VT E  + AK  L+++  AG+G D+VDL AA   G  V     +N V+ AE  +  +  +
Sbjct: 134 VTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASM 193

Query: 169 VRNFLPGHHQVINGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHD 228
            RN       +  G+W  +   +    L GKT+  +G G++G  + +R K     ++ HD
Sbjct: 194 ARNVAQADASIKAGKWERS--KYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHD 253

Query: 229 RLKIDPELEKQIGAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVN 288
                 +  + +G       D  +   D + ++ PLT  T+ +FN E  +K KKGV ++N
Sbjct: 254 PY-APADRARALGVDL-VSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLIN 313

Query: 289 NARGAIMDTQAVVDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQ 348
            ARG ++D  A+V A ++G +   + DV+  +P  KD       N  +TPH+  +T +AQ
Sbjct: 314 VARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQ 373

Query: 349 LRYAAGVKDMLERYFKGE 366
              A  + + +    KGE
Sbjct: 374 EGVAIEIAEAVAGALKGE 387

BLAST of MC08g1304 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 112.8 bits (281), Expect = 5.9e-25
Identity = 74/246 (30.08%), Postives = 117/246 (47.56%), Query Frame = 0

Query: 120 LQLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 179
           L+++  AG+G D+VDL+AA   G  V     +N ++ AE  +  +  + RN       V 
Sbjct: 125 LKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVK 184

Query: 180 NGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQI 239
            GEW      +    L GKT+  +G G++G  + +R K     ++ HD      +    I
Sbjct: 185 AGEWK--RNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPY-APADRAHAI 244

Query: 240 GAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAV 299
           G       D  L   D + ++ PLT  T  + N E  AK KKGV IVN ARG ++D  A+
Sbjct: 245 GVDL-VSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDAL 304

Query: 300 VDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLE 359
           V A ++G +   + DV+  +P  KD          +TPH+  +T++AQ   A  + + + 
Sbjct: 305 VRALDAGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVV 364

Query: 360 RYFKGE 366
               GE
Sbjct: 365 GALNGE 366

BLAST of MC08g1304 vs. TAIR 10
Match: AT1G17745.2 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 108.2 bits (269), Expect = 1.5e-23
Identity = 78/283 (27.56%), Postives = 127/283 (44.88%), Query Frame = 0

Query: 109 VTAERIKKAK-NLQLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILIL 168
           VT E  + AK  L+++  AG+G D+VDL AA   G  V     +N V+ AE  +  +  +
Sbjct: 134 VTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASM 193

Query: 169 VRNFLPGHHQVING-----------EWNVAGIAHR--------------AYDLEGKTVGT 228
            RN       +  G            WN     H+                 L GKT+  
Sbjct: 194 ARNVAQADASIKAGTLNYLFLVLLLRWNCRQSKHQYTIETETEKRSKYVGVSLVGKTLAV 253

Query: 229 VGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIGAQFEEDLDAMLPKCDVLVINTP 288
           +G G++G  + +R K     ++ HD      +  + +G       D  +   D + ++ P
Sbjct: 254 MGFGKVGTEVARRAKGLGMTVISHDPY-APADRARALGVDL-VSFDQAISTADFVSLHMP 313

Query: 289 LTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVVDACNSGHIGGYSGDVWNPQPAP 348
           LT  T+ +FN E  +K KKGV ++N ARG ++D  A+V A ++G +   + DV+  +P  
Sbjct: 314 LTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPS 373

Query: 349 KDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLERYFKGE 366
           KD       N  +TPH+  +T +AQ   A  + + +    KGE
Sbjct: 374 KDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALKGE 414

BLAST of MC08g1304 vs. TAIR 10
Match: AT1G72190.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.9e-23
Identity = 77/257 (29.96%), Postives = 123/257 (47.86%), Query Frame = 0

Query: 114 IKKAKNLQLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLP 173
           I +A N++L++  G+G D VD++AA   G+ VA +      + A    M I +++     
Sbjct: 110 ISRASNIKLIMQYGVGLDGVDIDAATKHGIKVARIPSEGTGNAASCSEMAIYLMLGLLKK 169

Query: 174 GHHQVINGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDR----- 233
            +   I+    + G       L GKTV  +G G IG  L +RLKPF   ++   R     
Sbjct: 170 QNEMQISLRNRLLG-EPTGDTLLGKTVFILGYGNIGIELAKRLKPFGSRVIATKRFWPAS 229

Query: 234 -LKIDPELEKQIGAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVN 293
            +  D  L  + G+   ED+     K D++V+   L  +T  + NKE I   KKG L+VN
Sbjct: 230 IVDSDSRLVDEKGS--HEDIYTFAGKADIVVVCLRLNKETAEIVNKEFICSMKKGALLVN 289

Query: 294 NARGAIMDTQAVVDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQ 353
            ARG +++ ++      SGH+GG   DV   +P   + P     N  +TPH++G T  + 
Sbjct: 290 IARGGLINYESAFQNLESGHLGGLGIDVAWSEPFDPNDPILKFKNVIITPHVAGVTEYSY 349

Query: 354 LRYAAGVKDMLERYFKG 365
              A  V D+  +  +G
Sbjct: 350 RSMAKIVGDLALQLHEG 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q075119.4e-19386.46Formate dehydrogenase, mitochondrial OS=Solanum tuberosum OX=4113 GN=FDH1 PE=1 S... [more]
Q9S7E44.7e-18483.12Formate dehydrogenase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=37... [more]
Q9SXP21.8e-18382.64Formate dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9ZRI82.6e-18285.47Formate dehydrogenase, mitochondrial OS=Hordeum vulgare OX=4513 PE=2 SV=1[more]
Q67U692.7e-17978.91Formate dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
XP_022158921.11.74e-278100.00formate dehydrogenase, mitochondrial [Momordica charantia][more]
XP_038904948.18.50e-27096.88formate dehydrogenase, mitochondrial [Benincasa hispida][more]
XP_004141089.11.21e-26996.09formate dehydrogenase, mitochondrial [Cucumis sativus] >KGN59666.1 hypothetical ... [more]
XP_008443517.12.00e-26895.83PREDICTED: formate dehydrogenase, mitochondrial [Cucumis melo] >KAA0053673.1 for... [more]
XP_022934681.11.64e-26795.05formate dehydrogenase, mitochondrial isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1E2C88.43e-279100.00Formate dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A0A0LHY65.85e-27096.09Formate dehydrogenase, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_3G836500 ... [more]
A0A5D3CRJ99.69e-26995.83Formate dehydrogenase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3B8Z59.69e-26995.83Formate dehydrogenase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103487087 PE=... [more]
A0A6J1F2G87.96e-26895.05Formate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114417... [more]
Match NameE-valueIdentityDescription
AT5G14780.13.3e-18583.12formate dehydrogenase [more]
AT1G17745.13.2e-2629.46D-3-phosphoglycerate dehydrogenase [more]
AT4G34200.15.9e-2530.08D-3-phosphoglycerate dehydrogenase [more]
AT1G17745.21.5e-2327.56D-3-phosphoglycerate dehydrogenase [more]
AT1G72190.11.9e-2329.96D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 37..380
e-value: 2.0E-164
score: 548.7
NoneNo IPR availableGENE3D3.40.50.720coord: 152..342
e-value: 2.0E-164
score: 548.7
NoneNo IPR availablePANTHERPTHR42938:SF26FORMATE DEHYDROGENASE CHLOROPLASTIC/MITOCHONDRIALcoord: 25..383
NoneNo IPR availablePANTHERPTHR42938FORMATE DEHYDROGENASE 1coord: 25..383
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 48..173
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 162..340
e-value: 1.5E-51
score: 174.3
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 69..366
e-value: 2.5E-15
score: 57.2
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00670D_2_HYDROXYACID_DH_2coord: 250..272
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00671D_2_HYDROXYACID_DH_3coord: 279..295
IPR029752D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1PROSITEPS00065D_2_HYDROXYACID_DH_1coord: 200..227
IPR033689NAD-dependent formate dehydrogenaseHAMAPMF_03210Formate_dehydrogenasecoord: 36..384
score: 59.25185
IPR033689NAD-dependent formate dehydrogenaseCDDcd05302FDHcoord: 36..383
e-value: 0.0
score: 655.166
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 155..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1304.1MC08g1304.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042183 formate catabolic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0009326 formate dehydrogenase complex
cellular_component GO:0005739 mitochondrion
molecular_function GO:0008863 formate dehydrogenase (NAD+) activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor