MC08g1219 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1219
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionLipase
LocationMC08: 10501396 .. 10509470 (-)
RNA-Seq ExpressionMC08g1219
SyntenyMC08g1219
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAATTAAGGAAAGTGAGGGAAGGATGATGGATGAATCCATCGTAACCGGCGGAATTTTAGTTTACCAATTTTAGCCTCAGTTACAGCCACGTCATCAGGTGGGACCCACCCATTTGACTTTCAAATTTCATTGGCTTTCATTTCACACTCTTTTCACTCTCTCTCACTATAAAAACCCCCAAAAAAGATTACAGAGAGACATACAGAGAGAGAGAGAGAGTTGGTGGTGGCCATGGCGTTTTCCGTCGTGGTCGCGGTGGCGCTGCTGGTTCTCGGCTGCTGCTGCGACGGAGGCCACGCCGGCGTTGTGGCTCCGCCGGAGGCGTTCGGTATCTGCCCTTCTGCTGTGACAATTCACGGCTATAAGTGCCAGGAAATTCAGGTATATATATATATACACAAACATTAATATATATATATATATATATATATATTTGATAATTAATAATAATTTTTTTTTGAAGAAATAATAAAGATTTTAACCCTATAACATTCACGTTTCTATTTCTTGTTTATTTCTTTTCGTTTTCTATTTTTTTCAAATATAAACATATGTTTTTCCTTCCTCCTCCTCATCCTTTTTCTTTTTCTTTGAAAAATTTGAGCAAAATTTAAAACTCAGAACCGTAACATTTTTTTTATTGTTTTTCGATTTTTTTAGAAACATAAAGCATATCCTTCCCTCTTCACCATCCTTTTTCCTTTTCTTTCTTCCTAAAGAAAAAACTCGAGCATATAATATAAATCTAACTATTTAATATGTTAATATTAAAAAGTTCTAAACTTAGAGCCGGTAAATTGTTTATGGTTTATTGTTTCCATTTTTTTTTTAAAAATATAAGCGGTAACTTACCTATTTATGGTTTTTAATTTTTTCATAACATAAATAAAAATATTATTTTATTATTTTTTACTTATAAATGGCATAATAAACATTTTAAAAAGTAAGTATATGATAATTTTTTTGAATTTAAGATTTAATAAGAAATTGTTATCAAAGGTGTAAAAACAAAAACGTCTGTTATCATATTTTTATTTAAAGTGTTTTATTCCAAAGGGAGGCATAGTAATTTCAAANTTAAATTATATATATATATATATATATATATATTAGTATAATATATGGGGTGGGGATGAAGTTCGAAATCTTTTAATTAGAGATATGTCAATTTCCACTGAGATATATTAAGCACTTTAAATTATAATTATACTACATATATACTATTAAAAATTGAGATCGTGCACGTAATTTTTTTTTTACAAGAAGAAATCCTAATTTTAAATATTTATCAAACACATGTACAATAAAAATTAAATCAACATTTCTATAGTATTATATAAAAAAAAGTAAAAAAAAAAAAAATTCCACTAAACATCTTTAGTACAAACATAATTTTAATATTAACCATTTTTAATGGGTTACCTGAGTTAACATGATAGGTGACGACGGAAGATGGGTACATTCTGAACCTACAGAGAATCCCAGAAGGTCGCCGTGGAGGCGGACCCAAGAAGAAACAGCCCGTCATTATACAGCATGGTGTTCTTGTGGTAATTTTTCTTTTTTTTCCTTTTTAGTTTCTCTCACTTTAATACAGATAATTCTTTTTCGTAAGTAGACTCTGATCGGATGCTCATTTGAGCGTTCGTTTTGAACTTTGATATCATATTAATATTCGTAGTTCATGTCTGTTATAAACCTATACAATGTTCATTCTTTTATCAATGTGAGATCAAGTAGATATTTTGAAAATTATTGAGTTCGATGAATAGAGATTCGAATACTCAATCGTTTGATTGATGATATATATTTTAACTGGTTGACTAGATCTAAATCAGTTTTATAAAAAAACGTATATGTAATAAAGTCACCTAAGAGACTTAATTTTTTTGAATTATAAGGTTTTTTTTTTTCATACAACAATAATTATAATAAGTTTGGGTTGTGGGTATGATGGACATTAATTATTGTAAAGGAAATTTGGGTTTCCATTATATATTTTGGCACTTTGAGTGAGTTGCACGTGTTCATTTTTTTTTTCATTGCTCTCACAAATTTGCAGGTGTCATTTTTTTTAAAAAAAAAATTATTCTCTCTCTTTTTTTTTTAGTGCAGCTAAGGGGATAAATTTTCTTTTTCTTTTACCCATACCTGGACTATTAAAGTATATATCTATGAAAAAAAAAGTCAGAAATTCGTGTTCACACTTAGAACTCAATTTTTTTTTCTCAAGTTTTAATATTCTAAAGTAAGGGGCCACTGTCTATTGTGTCTATGATAATGTCCACTTACGCTTTAGGTTATTGAGTAAAAGAAAAATCACTTTTATCCTATAGTTAACTAATTTTTGCTCAAAGTTTAATTAATAAATTATCTAGATTTCGAGACTAGAGAGTGAAAAATAGAAAGAAAAACATTATTTCTCGGAGAAATAGTAGATTGAAAATTTTGTGAAATGTTCAAAACACAAACTAATACTTATGAAGAACCAATATTTTTTTAGGTGAAAATAAAAATAAAAGTAAGAAAACAGGTTTTTGAATCGATATTTGAAATATATTTTCAAATGAAAAATATAGTCTAAATTTATAGGAAAATAAGACACCCAGTATCATTCCAAGTATTAATTGTTAAACTAAAAAAAGACAAATTTTTATTATGTTTTCATTAAGTTATACGTTGAAAAAAAAAATATAAATTAAATAAGTTTTTCTGATGAAATATAAATTAAATGAGTTGGAGGATAAAGTTATTTAACCTATATCGAATAATACGGCGCCGTTCCGTACATTAATCTGATTGATAGTTCCAAGAATGGATTCTTCTTCTTCATCTGCATTTCGAAAACATCACTCTTTTTTTTGAAAACTTCGAAGTCCTGACTTCTTTTTTCATATAATGTATAAGAATATTATCTATTATATTTTTGTTTTTAATTATAAATGTAACTATAAAAAAAATAAAAATTTAGGTGATATATGTTAAATTAGAAATTTTCTCTCTAGAATTATGACATAATTTTAATTTAGTCCAGATTCAATTGTTCATTTCTTATAAAAGTCAAAACCTTGTAATTTATAATTTTTTTTAGTAAAAAAAACCTTGTAATTTATTTGTTACCATATTAATACATTTTTTATATGGCAAATGAGTTGATGTTTTCTATACAACTAAATATGTTAGTTGATGACTATATCCAGATTTAAGGGGTAGAAAATAAAAGAAGATATATAAATTTAATATTAAATTGAAACTTTTAAAACTATACGAAATTAGCCTAGTAAAGGTTTTAAAGGAAAAATTGACCTTATCTTGAGCTATGCCATATGTTTTCTTTTTTGATGGTGTAGGACGGGACAACGTGGCTATTGAATTCTCCAGATCAGAACTTGCCGATGATCTTAGCCGACAAGGGATTTGATGTGTGGATCACCAACACTCGAGGCACACGCTTCAGCCGTCGACACACCTCCCTCGACCCTGACTCCAAGGAATATTGGAATTGGTCATGGGACCAACTCGTCGCATATGATCTCCCAGCTGTTTTCGACCATGTCTCGCAAGAAACCGGCCAGAAGATCCACTACGTCGGCCACTCTCTAGTAAGTTTCTTACTCGAGGCGAGACAATTGAACCCCCAGTTTATCGTTTCAAATTTTAGAAAATTATATTTCACTTTAGTACTTGAATTTTCAAGAATTTTCTAATTTAATTCTTAGAGATATCCTATACAATAAGTTTGTATAAGATCAGACCGCGAAATAATTAATCTTTAGTTTATAACATCGTTAGCTAATAGATTAATATTTGAGTGAGTTATGGATTTCTGCATGTTAAAACTCGTCGTTTAATTTGTATGGGTGAGAGTGGTTCAAGTCGTCGACTCAATATTCTAAAATATTTATTTAGGTCCTTACTTATTTTAAAGTTATTATATTTTAGTCCAACGTGTACCACGAGTTTCAAAAATAAACATTTGATCTTTACTTTTGTTTTGGGTTTAACAAATGGATAAAATAGAGAATATGTATAATATTGTTGTAGGTGAAAAAATAATAAAGATAAAAGTAGTTACTTTTTTTTTAAGTTAAGATACTAAATTATAAATTCTGCTATTGTAATAACAAAAAATAATTAGCTTTTTTTTATTTTATTTTTTTTTACGAATTTAGAGACCGAAATCGATAATTGGTAAGTTCATGGACCAAAACCAAAAAAGTTATTGAGGGTTTGAAAATTAAAATGGAATATATATATAGATTATAAAATTTTTTTTCTTTTTTTTGATGTGCCTCTGAAAAGAGTTATTATATGCTTCTAAACTTTTTTGGTGCTGTCCAACTGTCCATCCATTACTTATTCTTTTAAAAGTGAGTTAAAAAAATTAAGCCCATCAGTGGATAATAATTTAGCTTATAGCTTATGTTGTGAAAAATCTTTAAAAAATAAATGAATGTTAATTTTTATTATGTAATAATTTTGGTTTTTCGATCTGGATATATAAAAAACCTCGTTAAATCTTAACATTTCTCACTATATTATTACAAAATAAAGTTTAAGACTCCCCAGTACTATCTATAATAGGTAATTTTTATGATACATAATTCTTTTATATATATTTTTTAACATGTAGAGGTGAGCGCGTGAATTTGTGTCTTTTGATGGTAACCCAATACCATTAATTAGAAGATACACACTAAATAAATATCTCGATATTCTCTGCTTTACGTATTACAAAAATTATATATCATAAAAAATTACGTATTATACATAAAAAATGATGTGCTCAACTCAGTAAAATATGGCAAGAAGATGAGCTCCTATTTTAAACTGGACAGGGGACGCTCATAATTTTGGCATCATTGTCGGAGGGGAGGTTGGTGGACAAGCTCCGGTCGGTGGCGCTTCTGAGCCCAATTGCATATCTCAGCCACATGACCACCGCTATCGGCGCCTTGGCTGCCCGATCCTTCGTCGGTGAGGTTTGTTACCGTCACTCTCACTTTTTTGGATCTTTTTAAGGATTTGTTTAGTATCATTCTCGTTACTTGTTTCTATTTCATTTTTTATAATAAAAAATATATATAATTGTACGATTTCAAAGAATAGTAAAACAATATTTTTATTTATATGGAAACAAATTAAAAACGAAAATAAGATGTAAAAGGTCAGGTCAGTTTGATAATGTTTATGTTTCTTTGTTTCTTTTTTCCAATTTTCTTAAGAAATCTAAGTATTTTGCAACTAACTATGTTTTTTGTTTCTCATTTCTTGTGGATATTATCAAAACTGTATAAAAATTTTAGAAACAATCCGAACAAGTTTTTTTATTTCTTGTTCCTATTCAATTCATGGATGTTAAGACCATACAATTTATTTCTTTATTAAAAACAAAGAAACAAAAATAAGAAATAAGAAAGTTATCTTGTTTTTGTTGTTTCAAAAAACTTCTATATAATTTAGAGAATATTTCTACAAAATAAGAAAGAAAAAATCAAATGTTTATTTATTTATTTTTGATAAAATTAGAAAAAAAAATAAAAAATAATAACTTTACGAAACGGACCCTAATGAAATACAGTCTGCGCAACCGCGGTTTCTCTTTCTTGACGATGATAGAATTTATCTGATACTTTTTGCAGCTTACTACTCTGTTGGGCGTTGCAGAGTTCAATCCAAAAGGGTACATTTCCTTTTTCTTTTCCTTTTTCCTTTTTTTTAAATAGTTAAATTATAAATTTAATTGCCTTACGATAGATAACCCTTCATTTTTCTCATTTTAAAATTATATTTATTTTTCTACCATCTTGAAAATATATTTGACTTTCTAGCCAAATTTATAAAACATATATATTTGAGTTTTTAAGCTAAATTTTAAAAACAACGATAAAATTTTAAATTTTTTCGTAAAATTTGACCTAGATTTTAATATTTTTAAAAAAATATTATATAACAAAACAAATGAGTATATAGGTGAAATTGATGTTTTTTAATTTAATTCTTTAAAAACATAAATAAAAAACGAAATGGTTATTAATGGGCCTTAATTATTTATTTAGATCTAGATTTAATTTGGTCCCATGATTTTAAAATTTTCAATTTAATCTTTCGAATTTGGGTTAATTTTCAAATTTGATACTTATAATTTTGAAAGTTTATTAAACTTTCCAATTTGTTGAAAAATTTACTTATTTCCTCCTATAAAATAGAGTCAAATAACATAGTTTTGTCTAATATAAACTCCCACTCGCCTGGTAAAAAAATTATATTAATGTGGTGACTGTATGAATAATCTTTTATACTTGACTAAAATTAAAATTAAATCTTTTAAAATTTTATAGACGAAATTTCTAATTCGAATAGAAAATCAAAATAGTTTATACTATATACAAAACAACCTTAATTTAAGATTATTTTGACGAGTTCATTATGATACGACTCAAAGTTGTCCTACAAATTTATATATATAAGGTTTTATAGATCGAAGAAAAGAAAAATCGACCTTCCACACGAAGGACTATTAATTAACTCAGTGGTAGAACGTGTCCCTTATAATGGCGTCGAGGATTATTATTATGTATTTGTGATTTTGTTCTTGGGAAAGAAAGGTTGATATAAATTTAATTTGTGATTTTTTATACAAAAATTTAATTTTGTCGTTGAATGACAGGGAGGCTGTGGGGCATTTTCTGAAAGTTCTGTGTGCTAATCAAGGGATAAATTGCTACGACTTATTAACAGCTCTCACTGGTTTATTTCTCTGACTCCTTTTTTGTTTGGTTTTTTTTTCCTTTAATAATAGTCAATAAATTAAGTAATCGTGTGATTTTCTTTTTATCCCTACAACATTAACTTATAATTTAATATATGCAGGTCATAATTGTTGTCTCAATTCATCTACCGTTGGACTCTTTTTGATGAATGAACCTCAGTCAACGTCCACCAAGAACATGGTCCACTTAGCTCAAAGTAAGTTAATTTTTACGGATTAATAGGTTGATGGGCCGTTTAGTAAATGTATTAAATAAGGGTAAAATCGGTATTATAAAAGAATACTACATGCAGACATCTAAAAGTGTAAAATTAGAAAATTTAAAGACATTTGTGAAATTAAGTTTCAAAACAGCCCCGAAGACATTACCTCCGACTTTTATATATTCGAGATGCAAACTTGACATCTGTGTGGGCTGGTCTAAGCTTGAATAGTAGGCTAATGTTCAAGGTTGGTTTCTCTAATCAAAATTTTGACTTTCACCATATACTTCCATGCTTAAATTACATCCAGATTAGTATTAAGCATAGAAAGATGTAGGGTTTGAATAATATTGATTAAACTAACACATTCATGTTTTTGTTTTTTAGTTGCGAGACATGGGGTATTGGCAAAGTACAACTACGGAAAAATATTCAACTTATTTCACTACAAAGAACTGAGGCCGCCCATCTACAACCTCTCCAACATTCCCCACGACGTCCCCATCTTCATCAGCTATGGCGGCCGGGACGCCCTCTCCGACGTGCCCGACGTCACCCACCTCCTCGACCACTTCAAACTCCACGATGTTGACAAGTTCTCCGTTCAGTTCATCGATCGCTATGCCCATGCTGACTTCATCATGGGCGTTGATGCCCACGACGTTGTTTACAGCTCCCTCGTCGCCTTCTTTGATAAACATTAATCAAGATCCTATGAAAAATACTGAGGCCTTCACTGATGAGAAACTATCTAACCACTCTTTGACAACTCAGTAAAAAAATCTAAAATTAGAGAATTTTTTTAAAAATTAATGAGTCGTCAATTTTTTAGCGGTAGGATAAAATTAGCGAGGCTTCAGTACTTTTCATATTATATTATTCTCTCTCTTGATTTGATTTCAGGGTGTAGGGAAAGAGAACGGGGTTCCTTTGTGGTGGCCTCATGTATTGTGTTCTCTTTCATTCTGTTATATTTTGAATATGATATAGAATTTTGTAAATTCTTAATAAATCCAATAGTTTTAAGTTTTGAAACATTTCTCAATAATTGTAGTATTAAAAGTATATTTATTTTCCACCCTACTTAACTAAGCAC

mRNA sequence

GAAAAATTAAGGAAAGTGAGGGAAGGATGATGGATGAATCCATCGTAACCGGCGGAATTTTAGTTTACCAATTTTAGCCTCAGTTACAGCCACGTCATCAGGTGGGACCCACCCATTTGACTTTCAAATTTCATTGGCTTTCATTTCACACTCTTTTCACTCTCTCTCACTATAAAAACCCCCAAAAAAGATTACAGAGAGACATACAGAGAGAGAGAGAGAGTTGGTGGTGGCCATGGCGTTTTCCGTCGTGGTCGCGGTGGCGCTGCTGGTTCTCGGCTGCTGCTGCGACGGAGGCCACGCCGGCGTTGTGGCTCCGCCGGAGGCGTTCGGTATCTGCCCTTCTGCTGTGACAATTCACGGCTATAAGTGCCAGGAAATTCAGGTGACGACGGAAGATGGGTACATTCTGAACCTACAGAGAATCCCAGAAGGTCGCCGTGGAGGCGGACCCAAGAAGAAACAGCCCGTCATTATACAGCATGGTGTTCTTGTGGACGGGACAACGTGGCTATTGAATTCTCCAGATCAGAACTTGCCGATGATCTTAGCCGACAAGGGATTTGATGTGTGGATCACCAACACTCGAGGCACACGCTTCAGCCGTCGACACACCTCCCTCGACCCTGACTCCAAGGAATATTGGAATTGGTCATGGGACCAACTCGTCGCATATGATCTCCCAGCTGTTTTCGACCATGTCTCGCAAGAAACCGGCCAGAAGATCCACTACGTCGGCCACTCTCTAGGGACGCTCATAATTTTGGCATCATTGTCGGAGGGGAGGTTGGTGGACAAGCTCCGGTCGGTGGCGCTTCTGAGCCCAATTGCATATCTCAGCCACATGACCACCGCTATCGGCGCCTTGGCTGCCCGATCCTTCGTCGGTGAGCTTACTACTCTGTTGGGCGTTGCAGAGTTCAATCCAAAAGGGGAGGCTGTGGGGCATTTTCTGAAAGTTCTGTGTGCTAATCAAGGGATAAATTGCTACGACTTATTAACAGCTCTCACTGGTCATAATTGTTGTCTCAATTCATCTACCGTTGGACTCTTTTTGATGAATGAACCTCAGTCAACGTCCACCAAGAACATGGTCCACTTAGCTCAAATTGCGAGACATGGGGTATTGGCAAAGTACAACTACGGAAAAATATTCAACTTATTTCACTACAAAGAACTGAGGCCGCCCATCTACAACCTCTCCAACATTCCCCACGACGTCCCCATCTTCATCAGCTATGGCGGCCGGGACGCCCTCTCCGACGTGCCCGACGTCACCCACCTCCTCGACCACTTCAAACTCCACGATGTTGACAAGTTCTCCGTTCAGTTCATCGATCGCTATGCCCATGCTGACTTCATCATGGGCGTTGATGCCCACGACGTTGTTTACAGCTCCCTCGTCGCCTTCTTTGATAAACATTAATCAAGATCCTATGAAAAATACTGAGGCCTTCACTGATGAGAAACTATCTAACCACTCTTTGACAACTCAGTAAAAAAATCTAAAATTAGAGAATTTTTTTAAAAATTAATGAGTCGTCAATTTTTTAGCGGTAGGATAAAATTAGCGAGGCTTCAGTACTTTTCATATTATATTATTCTCTCTCTTGATTTGATTTCAGGGTGTAGGGAAAGAGAACGGGGTTCCTTTGTGGTGGCCTCATGTATTGTGTTCTCTTTCATTCTGTTATATTTTGAATATGATATAGAATTTTGTAAATTCTTAATAAATCCAATAGTTTTAAGTTTTGAAACATTTCTCAATAATTGTAGTATTAAAAGTATATTTATTTTCCACCCTACTTAACTAAGCAC

Coding sequence (CDS)

ATGGCGTTTTCCGTCGTGGTCGCGGTGGCGCTGCTGGTTCTCGGCTGCTGCTGCGACGGAGGCCACGCCGGCGTTGTGGCTCCGCCGGAGGCGTTCGGTATCTGCCCTTCTGCTGTGACAATTCACGGCTATAAGTGCCAGGAAATTCAGGTGACGACGGAAGATGGGTACATTCTGAACCTACAGAGAATCCCAGAAGGTCGCCGTGGAGGCGGACCCAAGAAGAAACAGCCCGTCATTATACAGCATGGTGTTCTTGTGGACGGGACAACGTGGCTATTGAATTCTCCAGATCAGAACTTGCCGATGATCTTAGCCGACAAGGGATTTGATGTGTGGATCACCAACACTCGAGGCACACGCTTCAGCCGTCGACACACCTCCCTCGACCCTGACTCCAAGGAATATTGGAATTGGTCATGGGACCAACTCGTCGCATATGATCTCCCAGCTGTTTTCGACCATGTCTCGCAAGAAACCGGCCAGAAGATCCACTACGTCGGCCACTCTCTAGGGACGCTCATAATTTTGGCATCATTGTCGGAGGGGAGGTTGGTGGACAAGCTCCGGTCGGTGGCGCTTCTGAGCCCAATTGCATATCTCAGCCACATGACCACCGCTATCGGCGCCTTGGCTGCCCGATCCTTCGTCGGTGAGCTTACTACTCTGTTGGGCGTTGCAGAGTTCAATCCAAAAGGGGAGGCTGTGGGGCATTTTCTGAAAGTTCTGTGTGCTAATCAAGGGATAAATTGCTACGACTTATTAACAGCTCTCACTGGTCATAATTGTTGTCTCAATTCATCTACCGTTGGACTCTTTTTGATGAATGAACCTCAGTCAACGTCCACCAAGAACATGGTCCACTTAGCTCAAATTGCGAGACATGGGGTATTGGCAAAGTACAACTACGGAAAAATATTCAACTTATTTCACTACAAAGAACTGAGGCCGCCCATCTACAACCTCTCCAACATTCCCCACGACGTCCCCATCTTCATCAGCTATGGCGGCCGGGACGCCCTCTCCGACGTGCCCGACGTCACCCACCTCCTCGACCACTTCAAACTCCACGATGTTGACAAGTTCTCCGTTCAGTTCATCGATCGCTATGCCCATGCTGACTTCATCATGGGCGTTGATGCCCACGACGTTGTTTACAGCTCCCTCGTCGCCTTCTTTGATAAACATTAA

Protein sequence

MAFSVVVAVALLVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNLQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASLSEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFLKVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAKYNYGKIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVDKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH
Homology
BLAST of MC08g1219 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.1e-138
Identity = 241/403 (59.80%), Postives = 299/403 (74.19%), Query Frame = 0

Query: 1   MAFSVVVAVALLVLGCCCDGGHAGVVAPP----EAFGICPSAVTIHGYKCQEIQVTTEDG 60
           +A SV++  AL +      G    +   P     A GIC S+V I GYKC+E  V T+DG
Sbjct: 14  LALSVLIFFALSLKTLEARGTFGRLAGQPPQRTAAGGICASSVHIFGYKCEEHDVVTQDG 73

Query: 61  YILNLQRIPEGRRG---GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVW 120
           YILN+QRIPEGR G   G   K+QPV+IQHG+LVDG +WLLN  DQNLP+ILAD+GFDVW
Sbjct: 74  YILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGMSWLLNPADQNLPLILADQGFDVW 133

Query: 121 ITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGT 180
           + NTRGTRFSRRH  L+P  + +WNW+WD+LV+YDLPA+FDH+   TGQKIHY+GHSLGT
Sbjct: 134 MGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLPAMFDHIHGLTGQKIHYLGHSLGT 193

Query: 181 LIILASLSEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKG 240
           LI  AS SE  LVD++RS A+LSP+AYLSHMTT IG +AA++F+ E T++LG  EFNPK 
Sbjct: 194 LIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPEFNPKS 253

Query: 241 EAVGHFLKVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIA 300
             VG F+K +C   GI+CYDL++ +TG NCCLN+ST+ LFL NEPQSTSTKNM+HLAQ  
Sbjct: 254 GLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIHLAQTV 313

Query: 301 RHGVLAKYNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLD 360
           R   L KYNYG    N+ HY +  PP YN+S IPH++P+F SYGG D+L+DV DV  LLD
Sbjct: 314 RDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLD 373

Query: 361 HFKLHDVDKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDK 396
            FK HD+DK +VQF+  YAHADFIMGV A DVVY+ +  FF +
Sbjct: 374 QFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQVATFFKR 416

BLAST of MC08g1219 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.1e-71
Identity = 136/362 (37.57%), Postives = 206/362 (56.91%), Query Frame = 0

Query: 34  ICPSAVTIHGYKCQEIQVTTEDGYILNLQRIPE--GRRGGGPKKKQPVIIQHGVLVDGTT 93
           +C   +    Y C E  + T+DGYIL LQR+     R   GP    PV++QHG+ + G  
Sbjct: 32  LCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQSGP----PVLLQHGLFMAGDV 91

Query: 94  WLLNSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAYDLPA 153
           W LNSP ++L  ILAD GFDVW+ N RGTR+S  H +L    KE+W+WSW  L  YDL  
Sbjct: 92  WFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYDLAE 151

Query: 154 VFDHVSQETGQKIHYVGHSLGTLIILASLSEGRLVDKLRSVALLSPIAYLSHMTTAIGAL 213
           +  ++   +  KI  VGHS GT++  A+L++  + + + + ALL PI+YL H+T  +   
Sbjct: 152 MIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPLVER 211

Query: 214 AARSFVGELTTLLGVAEFNPKGEAVGHFLKVLCANQGINCYDLLTALTGHNCCLNSSTVG 273
                + ++   LG+ + N + + +   +  LC    ++C D LT++TG NCC N+S + 
Sbjct: 212 MVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGH-MDCTDFLTSITGTNCCFNASKIE 271

Query: 274 LFLMNEPQSTSTKNMVHLAQIARHGVLAKYNYGKIFNLFHYKELRPPIYNLSNIPHDVPI 333
            +L  EP  +S KN+ HL Q+ R G  A+Y+YG   NL  Y   +PP + LS+IP  +P+
Sbjct: 272 YYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASLPM 331

Query: 334 FISYGGRDALSDVPDVTHLLDHFKLHDVDKFSVQFIDRYAHADFIMGVDAHDVVYSSLVA 393
           ++ YGG D L+DV DV H L            + +++ Y H DF++G  A + VY  ++ 
Sbjct: 332 WMGYGGTDGLADVTDVEHTLAELP----SSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQ 384

BLAST of MC08g1219 vs. ExPASy Swiss-Prot
Match: P80035 (Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 8.5e-56
Identity = 131/371 (35.31%), Postives = 208/371 (56.06%), Query Frame = 0

Query: 29  PEAFGICPSAVTIHGYKCQEIQVTTEDGYILNLQRIPEGRRGGGPKKKQPV-IIQHGVLV 88
           PE        +T  GY  +E +V TEDGYIL + RIP GR+      ++PV  +QHG+L 
Sbjct: 29  PEVTMNISQMITYWGYPAEEYEVVTEDGYILGIDRIPYGRKNSENIGRRPVAFLQHGLLA 88

Query: 89  DGTTWLLNSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAY 148
             T W+ N P+ +L  ILAD G+DVW+ N+RG  ++RR+    PDS E+W +S+D++  Y
Sbjct: 89  SATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKY 148

Query: 149 DLPAVFDHVSQETGQ-KIHYVGHSLGTLI-ILASLSEGRLVDKLRSVALLSPIAYLSHMT 208
           DLPA  D + ++TGQ K+HYVGHS GT I  +A  +  +L  ++++   L+P+A + +  
Sbjct: 149 DLPATIDFILKKTGQDKLHYVGHSQGTTIGFIAFSTNPKLAKRIKTFYALAPVATVKYTE 208

Query: 209 TAIGAL-AARSFVGELTTLLGVAEFNPKGEAVGHFLKVLCANQGIN--CYDLLTALTGHN 268
           T +  L    SF+ +L  + G   F P           +C+ + ++  C + L  + G +
Sbjct: 209 TLLNKLMLVPSFLFKL--IFGNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFD 268

Query: 269 CC-LNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAKYNYGK-IFNLFHYKELRPPIY 328
              LN S + ++L + P  TS +N++H +Q  + G    +++G  + N+ HY +  PP Y
Sbjct: 269 TMNLNMSRLDVYLSHNPAGTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYY 328

Query: 329 NLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVDKFSVQFIDRYAHADFIMGVD 388
           NL+++   VPI +  GG D L+D  DV  LL   KL ++     + I  Y H DFI  +D
Sbjct: 329 NLTDM--HVPIAVWNGGNDLLADPHDVDLLLS--KLPNL--IYHRKIPPYNHLDFIWAMD 388

Query: 389 AHDVVYSSLVA 392
           A   VY+ +V+
Sbjct: 389 APQAVYNEIVS 391

BLAST of MC08g1219 vs. ExPASy Swiss-Prot
Match: P07098 (Gastric triacylglycerol lipase OS=Homo sapiens OX=9606 GN=LIPF PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 5.5e-55
Identity = 131/376 (34.84%), Postives = 207/376 (55.05%), Query Frame = 0

Query: 29  PEAFGICPSAVTIHGYKCQEIQVTTEDGYILNLQRIPEGRRGGGPKKKQPVI-IQHGVLV 88
           PE        +T  GY  +E +V TEDGYIL + RIP G++  G   ++PV+ +QHG+L 
Sbjct: 29  PEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQHGLLA 88

Query: 89  DGTTWLLNSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAY 148
             T W+ N P+ +L  ILAD G+DVW+ N+RG  ++RR+    PDS E+W +S+D++  Y
Sbjct: 89  SATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKY 148

Query: 149 DLPAVFDHVSQETGQK-IHYVGHSLGTLI-ILASLSEGRLVDKLRSVALLSPIAYLSHMT 208
           DLPA  D + ++TGQK +HYVGHS GT I  +A  +   L  ++++   L+P+A + +  
Sbjct: 149 DLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTK 208

Query: 209 TAIGALAARSFVGE--LTTLLGVAEFNPKGEAVGHFLKVLCANQGIN--CYDLLTALTGH 268
           + I  L    FV +     + G   F P           +C+ + +N  C + L  + G 
Sbjct: 209 SLINKL---RFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGF 268

Query: 269 NC-CLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAKYNYGK-IFNLFHYKELRPPI 328
           +    N+S + ++L + P  TS +NM H  Q  + G    Y++G  + N  HY + +PP 
Sbjct: 269 DSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPY 328

Query: 329 YNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVDKFSVQFIDRYAHADFIMGV 388
           YN++ +  +VPI +  GG+D L+D  DV  LL   KL ++     + I  Y H DFI  +
Sbjct: 329 YNVTAM--NVPIAVWNGGKDLLADPQDVGLLLP--KLPNL--IYHKEIPFYNHLDFIWAM 388

Query: 389 DAHDVVYSSLVAFFDK 396
           DA   VY+ +V+   +
Sbjct: 389 DAPQEVYNDIVSMISE 395

BLAST of MC08g1219 vs. ExPASy Swiss-Prot
Match: Q3YBN2 (Tear acid lipase-like protein OS=Mesocricetus auratus OX=10036 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 9.5e-55
Identity = 128/377 (33.95%), Postives = 203/377 (53.85%), Query Frame = 0

Query: 29  PEAFGICPSAVTIHGYKCQEIQVTTEDGYILNLQRIPEGRRG-GGPKKKQPVIIQHGVLV 88
           PEA+      V   GY  +E +  TEDGYIL L RIP G+        K+ V+ QHG+  
Sbjct: 27  PEAYMKVSKIVNHWGYTSEEYEAVTEDGYILPLNRIPHGKNNINSTAPKKVVLCQHGLFS 86

Query: 89  DGTTWLLNSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAY 148
               W+ N P  +L  ILAD GFDVW+ N+RG+ ++++H  LDP+SKE+W +S+D+++ Y
Sbjct: 87  TAGVWVSNPPSNSLAFILADAGFDVWMGNSRGSTWAKKHLYLDPNSKEFWAFSFDEMIKY 146

Query: 149 DLPAVFDHVSQETGQK-IHYVGHSLGTLIILASLSEG-RLVDKLRSVALLSPIAYLSHMT 208
           DLPA  + + ++TGQK I+Y+GHS G LI L + S   +L +K++   LL+PIA L H+ 
Sbjct: 147 DLPATINFILKKTGQKQIYYIGHSQGALIALGAFSTNQKLAEKIKLCFLLAPIATLKHVE 206

Query: 209 TAIGAL-----AARSFVGELTTLLGVAEFNPKGEAVGHFLKVLCANQGINCYDLLTALTG 268
             +  L      A   V      L    F+   +  G+       N G  C  +  ++TG
Sbjct: 207 GIVSLLPYFYPTAFKVVFSEKEFLSAVAFS---KLHGYSCNAKVINDG--CVAIFLSMTG 266

Query: 269 H-NCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAKYNYG-KIFNLFHYKELRPP 328
           +    LN S V +++ +    TS + ++H  Q  + GV   Y++G +  N+ HY +  PP
Sbjct: 267 YVPQHLNKSRVDVYIRHSLAGTSVQTLLHYRQAIKKGVFEAYDWGSQSLNMLHYNQTTPP 326

Query: 329 IYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVDKFSVQFIDRYAHADFIMG 388
           +YN+ ++   +P  +  GG+D+L+D  DV HL+         K +  F    +H DF +G
Sbjct: 327 LYNVEDM--KIPTAMWSGGKDSLADTKDVAHLVPKISNLIYHKITADF----SHLDFTVG 386

Query: 389 VDAHDVVYSSLVAFFDK 396
            +A+  V + ++   DK
Sbjct: 387 KNAY-YVSNDILKLLDK 391

BLAST of MC08g1219 vs. NCBI nr
Match: XP_022154145.1 (triacylglycerol lipase 2 [Momordica charantia])

HSP 1 Score: 813 bits (2101), Expect = 1.37e-297
Identity = 396/396 (100.00%), Postives = 396/396 (100.00%), Query Frame = 0

Query: 1   MAFSVVVAVALLVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILN 60
           MAFSVVVAVALLVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILN
Sbjct: 1   MAFSVVVAVALLVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILN 60

Query: 61  LQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 120
           LQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT
Sbjct: 61  LQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 120

Query: 121 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 180
           RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL
Sbjct: 121 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 180

Query: 181 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL 240
           SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL
Sbjct: 181 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL 240

Query: 241 KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK 300
           KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK
Sbjct: 241 KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK 300

Query: 301 YNYGKIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVD 360
           YNYGKIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVD
Sbjct: 301 YNYGKIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVD 360

Query: 361 KFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           KFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH
Sbjct: 361 KFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396

BLAST of MC08g1219 vs. NCBI nr
Match: XP_008450088.1 (PREDICTED: triacylglycerol lipase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 619 bits (1595), Expect = 2.45e-220
Identity = 300/397 (75.57%), Postives = 340/397 (85.64%), Query Frame = 0

Query: 3   FSVVVAVAL-LVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNL 62
           FSVV+ V L +VLG CC GGH  V+ P E  GIC SAVTI+GYKCQEIQVTT+DGYIL++
Sbjct: 7   FSVVMTVVLTVVLGGCCVGGHGVVIGPTEELGICASAVTIYGYKCQEIQVTTKDGYILSV 66

Query: 63  QRIPEGRRG-GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 122
           QRI EGRRG GG  KKQPVIIQHGVLVDG TWLLNSP+QNLPMILAD G+DVWI NTRGT
Sbjct: 67  QRISEGRRGNGGKSKKQPVIIQHGVLVDGVTWLLNSPEQNLPMILADDGYDVWIANTRGT 126

Query: 123 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 182
           RFSRRHT+L+P  + +WNWSWD+LV YDLPAVFDHV Q+T QKIHYVGHSLGTLI+LASL
Sbjct: 127 RFSRRHTTLNPTDQAFWNWSWDELVLYDLPAVFDHVFQQTSQKIHYVGHSLGTLIVLASL 186

Query: 183 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL 242
           SEG+LV++L SVA LSPIAYLSHMTT IG LAARS + E+  LLG+AEFNPKG+ VG  L
Sbjct: 187 SEGKLVNQLHSVAFLSPIAYLSHMTTPIGVLAARSLLPEVYGLLGIAEFNPKGKEVGELL 246

Query: 243 KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK 302
           KV C + G+NCYDLL+A TGHNCCLNSSTV LFL NEPQSTSTKNMVHLAQ  R GVLAK
Sbjct: 247 KVWCTHPGVNCYDLLSAFTGHNCCLNSSTVELFLKNEPQSTSTKNMVHLAQTVRSGVLAK 306

Query: 303 YNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDV 362
           YNYG+  +NL HY E+ PP+YNLSNIPHD+ IFISYGGRDALSDV DV  LLDHFKLHDV
Sbjct: 307 YNYGRTDYNLMHYGEINPPLYNLSNIPHDLAIFISYGGRDALSDVKDVNRLLDHFKLHDV 366

Query: 363 DKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           DKF+VQFI  YAHAD+IMGVDA+++VY+S+++FF KH
Sbjct: 367 DKFAVQFIQNYAHADYIMGVDANNIVYNSVISFFKKH 403

BLAST of MC08g1219 vs. NCBI nr
Match: XP_008450087.1 (PREDICTED: triacylglycerol lipase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 614 bits (1583), Expect = 1.71e-218
Identity = 300/398 (75.38%), Postives = 340/398 (85.43%), Query Frame = 0

Query: 3   FSVVVAVAL-LVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNL 62
           FSVV+ V L +VLG CC GGH  V+ P E  GIC SAVTI+GYKCQEIQVTT+DGYIL++
Sbjct: 7   FSVVMTVVLTVVLGGCCVGGHGVVIGPTEELGICASAVTIYGYKCQEIQVTTKDGYILSV 66

Query: 63  QRIPEGRRG-GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 122
           QRI EGRRG GG  KKQPVIIQHGVLVDG TWLLNSP+QNLPMILAD G+DVWI NTRGT
Sbjct: 67  QRISEGRRGNGGKSKKQPVIIQHGVLVDGVTWLLNSPEQNLPMILADDGYDVWIANTRGT 126

Query: 123 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 182
           RFSRRHT+L+P  + +WNWSWD+LV YDLPAVFDHV Q+T QKIHYVGHSLGTLI+LASL
Sbjct: 127 RFSRRHTTLNPTDQAFWNWSWDELVLYDLPAVFDHVFQQTSQKIHYVGHSLGTLIVLASL 186

Query: 183 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGE-LTTLLGVAEFNPKGEAVGHF 242
           SEG+LV++L SVA LSPIAYLSHMTT IG LAARS + E +  LLG+AEFNPKG+ VG  
Sbjct: 187 SEGKLVNQLHSVAFLSPIAYLSHMTTPIGVLAARSLLPEKVYGLLGIAEFNPKGKEVGEL 246

Query: 243 LKVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLA 302
           LKV C + G+NCYDLL+A TGHNCCLNSSTV LFL NEPQSTSTKNMVHLAQ  R GVLA
Sbjct: 247 LKVWCTHPGVNCYDLLSAFTGHNCCLNSSTVELFLKNEPQSTSTKNMVHLAQTVRSGVLA 306

Query: 303 KYNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHD 362
           KYNYG+  +NL HY E+ PP+YNLSNIPHD+ IFISYGGRDALSDV DV  LLDHFKLHD
Sbjct: 307 KYNYGRTDYNLMHYGEINPPLYNLSNIPHDLAIFISYGGRDALSDVKDVNRLLDHFKLHD 366

Query: 363 VDKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           VDKF+VQFI  YAHAD+IMGVDA+++VY+S+++FF KH
Sbjct: 367 VDKFAVQFIQNYAHADYIMGVDANNIVYNSVISFFKKH 404

BLAST of MC08g1219 vs. NCBI nr
Match: XP_011651565.1 (triacylglycerol lipase 2 isoform X2 [Cucumis sativus] >KGN58188.1 hypothetical protein Csa_017643 [Cucumis sativus])

HSP 1 Score: 607 bits (1565), Expect = 9.04e-216
Identity = 295/397 (74.31%), Postives = 337/397 (84.89%), Query Frame = 0

Query: 3   FSVVVAVAL-LVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNL 62
           FSVV+ V L +VLG CC GGH  V+   +  GIC SAVT +GYKCQEIQVTT+DGYIL++
Sbjct: 7   FSVVMTVVLTVVLGACCGGGHGVVIGSTDELGICASAVTGYGYKCQEIQVTTKDGYILSV 66

Query: 63  QRIPEGRRG-GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 122
           QRI EGRRG GG  KKQP+IIQHGVLVDG TWLLNSP+QNLPMILAD G+DVWI NTRGT
Sbjct: 67  QRISEGRRGNGGKSKKQPIIIQHGVLVDGVTWLLNSPEQNLPMILADNGYDVWIANTRGT 126

Query: 123 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 182
           RFSRRHT+L+P  + +WNWSWD+LV YDLPAVFDHVSQ+T QKIHYVGHSLGTLI+LASL
Sbjct: 127 RFSRRHTTLNPTDQAFWNWSWDELVLYDLPAVFDHVSQQTSQKIHYVGHSLGTLIVLASL 186

Query: 183 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL 242
           SEG+LV +L+SVA LSPIAYLSHMTT IG LAARS + E+T LLG+AEFNPKG+ VG  L
Sbjct: 187 SEGKLVSQLQSVAFLSPIAYLSHMTTPIGVLAARSLLPEVTALLGIAEFNPKGKEVGDLL 246

Query: 243 KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK 302
           K LCA+ G+NCYDLL+A TG NCCLNSSTV LFL NEPQSTSTKNMVHLAQ  R GVLAK
Sbjct: 247 KSLCAHPGVNCYDLLSAFTGVNCCLNSSTVELFLKNEPQSTSTKNMVHLAQTVRSGVLAK 306

Query: 303 YNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDV 362
           YNYG I +NL HY E+ PP+YNLS+IPHD+ IFISYGG+DALSDV DV  LLDHFKLHDV
Sbjct: 307 YNYGNINYNLMHYGEINPPLYNLSSIPHDLAIFISYGGQDALSDVKDVDLLLDHFKLHDV 366

Query: 363 DKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           DK +V FI  YAHAD+IMGVDA+++VY+ L++FF KH
Sbjct: 367 DKLTVHFIQNYAHADYIMGVDANNIVYNPLISFFKKH 403

BLAST of MC08g1219 vs. NCBI nr
Match: XP_011651564.1 (triacylglycerol lipase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 602 bits (1553), Expect = 6.29e-214
Identity = 295/398 (74.12%), Postives = 337/398 (84.67%), Query Frame = 0

Query: 3   FSVVVAVAL-LVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNL 62
           FSVV+ V L +VLG CC GGH  V+   +  GIC SAVT +GYKCQEIQVTT+DGYIL++
Sbjct: 7   FSVVMTVVLTVVLGACCGGGHGVVIGSTDELGICASAVTGYGYKCQEIQVTTKDGYILSV 66

Query: 63  QRIPEGRRG-GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 122
           QRI EGRRG GG  KKQP+IIQHGVLVDG TWLLNSP+QNLPMILAD G+DVWI NTRGT
Sbjct: 67  QRISEGRRGNGGKSKKQPIIIQHGVLVDGVTWLLNSPEQNLPMILADNGYDVWIANTRGT 126

Query: 123 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 182
           RFSRRHT+L+P  + +WNWSWD+LV YDLPAVFDHVSQ+T QKIHYVGHSLGTLI+LASL
Sbjct: 127 RFSRRHTTLNPTDQAFWNWSWDELVLYDLPAVFDHVSQQTSQKIHYVGHSLGTLIVLASL 186

Query: 183 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGE-LTTLLGVAEFNPKGEAVGHF 242
           SEG+LV +L+SVA LSPIAYLSHMTT IG LAARS + E +T LLG+AEFNPKG+ VG  
Sbjct: 187 SEGKLVSQLQSVAFLSPIAYLSHMTTPIGVLAARSLLPEKVTALLGIAEFNPKGKEVGDL 246

Query: 243 LKVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLA 302
           LK LCA+ G+NCYDLL+A TG NCCLNSSTV LFL NEPQSTSTKNMVHLAQ  R GVLA
Sbjct: 247 LKSLCAHPGVNCYDLLSAFTGVNCCLNSSTVELFLKNEPQSTSTKNMVHLAQTVRSGVLA 306

Query: 303 KYNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHD 362
           KYNYG I +NL HY E+ PP+YNLS+IPHD+ IFISYGG+DALSDV DV  LLDHFKLHD
Sbjct: 307 KYNYGNINYNLMHYGEINPPLYNLSSIPHDLAIFISYGGQDALSDVKDVDLLLDHFKLHD 366

Query: 363 VDKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           VDK +V FI  YAHAD+IMGVDA+++VY+ L++FF KH
Sbjct: 367 VDKLTVHFIQNYAHADYIMGVDANNIVYNPLISFFKKH 404

BLAST of MC08g1219 vs. ExPASy TrEMBL
Match: A0A6J1DIT2 (Lipase OS=Momordica charantia OX=3673 GN=LOC111021471 PE=3 SV=1)

HSP 1 Score: 813 bits (2101), Expect = 6.63e-298
Identity = 396/396 (100.00%), Postives = 396/396 (100.00%), Query Frame = 0

Query: 1   MAFSVVVAVALLVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILN 60
           MAFSVVVAVALLVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILN
Sbjct: 1   MAFSVVVAVALLVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILN 60

Query: 61  LQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 120
           LQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT
Sbjct: 61  LQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 120

Query: 121 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 180
           RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL
Sbjct: 121 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 180

Query: 181 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL 240
           SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL
Sbjct: 181 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL 240

Query: 241 KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK 300
           KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK
Sbjct: 241 KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK 300

Query: 301 YNYGKIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVD 360
           YNYGKIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVD
Sbjct: 301 YNYGKIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVD 360

Query: 361 KFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           KFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH
Sbjct: 361 KFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396

BLAST of MC08g1219 vs. ExPASy TrEMBL
Match: A0A1S3BMV9 (Lipase OS=Cucumis melo OX=3656 GN=LOC103491778 PE=3 SV=1)

HSP 1 Score: 619 bits (1595), Expect = 1.19e-220
Identity = 300/397 (75.57%), Postives = 340/397 (85.64%), Query Frame = 0

Query: 3   FSVVVAVAL-LVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNL 62
           FSVV+ V L +VLG CC GGH  V+ P E  GIC SAVTI+GYKCQEIQVTT+DGYIL++
Sbjct: 7   FSVVMTVVLTVVLGGCCVGGHGVVIGPTEELGICASAVTIYGYKCQEIQVTTKDGYILSV 66

Query: 63  QRIPEGRRG-GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 122
           QRI EGRRG GG  KKQPVIIQHGVLVDG TWLLNSP+QNLPMILAD G+DVWI NTRGT
Sbjct: 67  QRISEGRRGNGGKSKKQPVIIQHGVLVDGVTWLLNSPEQNLPMILADDGYDVWIANTRGT 126

Query: 123 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 182
           RFSRRHT+L+P  + +WNWSWD+LV YDLPAVFDHV Q+T QKIHYVGHSLGTLI+LASL
Sbjct: 127 RFSRRHTTLNPTDQAFWNWSWDELVLYDLPAVFDHVFQQTSQKIHYVGHSLGTLIVLASL 186

Query: 183 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL 242
           SEG+LV++L SVA LSPIAYLSHMTT IG LAARS + E+  LLG+AEFNPKG+ VG  L
Sbjct: 187 SEGKLVNQLHSVAFLSPIAYLSHMTTPIGVLAARSLLPEVYGLLGIAEFNPKGKEVGELL 246

Query: 243 KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK 302
           KV C + G+NCYDLL+A TGHNCCLNSSTV LFL NEPQSTSTKNMVHLAQ  R GVLAK
Sbjct: 247 KVWCTHPGVNCYDLLSAFTGHNCCLNSSTVELFLKNEPQSTSTKNMVHLAQTVRSGVLAK 306

Query: 303 YNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDV 362
           YNYG+  +NL HY E+ PP+YNLSNIPHD+ IFISYGGRDALSDV DV  LLDHFKLHDV
Sbjct: 307 YNYGRTDYNLMHYGEINPPLYNLSNIPHDLAIFISYGGRDALSDVKDVNRLLDHFKLHDV 366

Query: 363 DKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           DKF+VQFI  YAHAD+IMGVDA+++VY+S+++FF KH
Sbjct: 367 DKFAVQFIQNYAHADYIMGVDANNIVYNSVISFFKKH 403

BLAST of MC08g1219 vs. ExPASy TrEMBL
Match: A0A1S3BPG0 (Lipase OS=Cucumis melo OX=3656 GN=LOC103491778 PE=3 SV=1)

HSP 1 Score: 614 bits (1583), Expect = 8.27e-219
Identity = 300/398 (75.38%), Postives = 340/398 (85.43%), Query Frame = 0

Query: 3   FSVVVAVAL-LVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNL 62
           FSVV+ V L +VLG CC GGH  V+ P E  GIC SAVTI+GYKCQEIQVTT+DGYIL++
Sbjct: 7   FSVVMTVVLTVVLGGCCVGGHGVVIGPTEELGICASAVTIYGYKCQEIQVTTKDGYILSV 66

Query: 63  QRIPEGRRG-GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 122
           QRI EGRRG GG  KKQPVIIQHGVLVDG TWLLNSP+QNLPMILAD G+DVWI NTRGT
Sbjct: 67  QRISEGRRGNGGKSKKQPVIIQHGVLVDGVTWLLNSPEQNLPMILADDGYDVWIANTRGT 126

Query: 123 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 182
           RFSRRHT+L+P  + +WNWSWD+LV YDLPAVFDHV Q+T QKIHYVGHSLGTLI+LASL
Sbjct: 127 RFSRRHTTLNPTDQAFWNWSWDELVLYDLPAVFDHVFQQTSQKIHYVGHSLGTLIVLASL 186

Query: 183 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGE-LTTLLGVAEFNPKGEAVGHF 242
           SEG+LV++L SVA LSPIAYLSHMTT IG LAARS + E +  LLG+AEFNPKG+ VG  
Sbjct: 187 SEGKLVNQLHSVAFLSPIAYLSHMTTPIGVLAARSLLPEKVYGLLGIAEFNPKGKEVGEL 246

Query: 243 LKVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLA 302
           LKV C + G+NCYDLL+A TGHNCCLNSSTV LFL NEPQSTSTKNMVHLAQ  R GVLA
Sbjct: 247 LKVWCTHPGVNCYDLLSAFTGHNCCLNSSTVELFLKNEPQSTSTKNMVHLAQTVRSGVLA 306

Query: 303 KYNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHD 362
           KYNYG+  +NL HY E+ PP+YNLSNIPHD+ IFISYGGRDALSDV DV  LLDHFKLHD
Sbjct: 307 KYNYGRTDYNLMHYGEINPPLYNLSNIPHDLAIFISYGGRDALSDVKDVNRLLDHFKLHD 366

Query: 363 VDKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           VDKF+VQFI  YAHAD+IMGVDA+++VY+S+++FF KH
Sbjct: 367 VDKFAVQFIQNYAHADYIMGVDANNIVYNSVISFFKKH 404

BLAST of MC08g1219 vs. ExPASy TrEMBL
Match: A0A0A0L8N7 (Lipase OS=Cucumis sativus OX=3659 GN=Csa_3G588470 PE=3 SV=1)

HSP 1 Score: 607 bits (1565), Expect = 4.38e-216
Identity = 295/397 (74.31%), Postives = 337/397 (84.89%), Query Frame = 0

Query: 3   FSVVVAVAL-LVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNL 62
           FSVV+ V L +VLG CC GGH  V+   +  GIC SAVT +GYKCQEIQVTT+DGYIL++
Sbjct: 7   FSVVMTVVLTVVLGACCGGGHGVVIGSTDELGICASAVTGYGYKCQEIQVTTKDGYILSV 66

Query: 63  QRIPEGRRG-GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGT 122
           QRI EGRRG GG  KKQP+IIQHGVLVDG TWLLNSP+QNLPMILAD G+DVWI NTRGT
Sbjct: 67  QRISEGRRGNGGKSKKQPIIIQHGVLVDGVTWLLNSPEQNLPMILADNGYDVWIANTRGT 126

Query: 123 RFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASL 182
           RFSRRHT+L+P  + +WNWSWD+LV YDLPAVFDHVSQ+T QKIHYVGHSLGTLI+LASL
Sbjct: 127 RFSRRHTTLNPTDQAFWNWSWDELVLYDLPAVFDHVSQQTSQKIHYVGHSLGTLIVLASL 186

Query: 183 SEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFL 242
           SEG+LV +L+SVA LSPIAYLSHMTT IG LAARS + E+T LLG+AEFNPKG+ VG  L
Sbjct: 187 SEGKLVSQLQSVAFLSPIAYLSHMTTPIGVLAARSLLPEVTALLGIAEFNPKGKEVGDLL 246

Query: 243 KVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAK 302
           K LCA+ G+NCYDLL+A TG NCCLNSSTV LFL NEPQSTSTKNMVHLAQ  R GVLAK
Sbjct: 247 KSLCAHPGVNCYDLLSAFTGVNCCLNSSTVELFLKNEPQSTSTKNMVHLAQTVRSGVLAK 306

Query: 303 YNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDV 362
           YNYG I +NL HY E+ PP+YNLS+IPHD+ IFISYGG+DALSDV DV  LLDHFKLHDV
Sbjct: 307 YNYGNINYNLMHYGEINPPLYNLSSIPHDLAIFISYGGQDALSDVKDVDLLLDHFKLHDV 366

Query: 363 DKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDKH 396
           DK +V FI  YAHAD+IMGVDA+++VY+ L++FF KH
Sbjct: 367 DKLTVHFIQNYAHADYIMGVDANNIVYNPLISFFKKH 403

BLAST of MC08g1219 vs. ExPASy TrEMBL
Match: A0A6J1F994 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111441988 PE=3 SV=1)

HSP 1 Score: 602 bits (1552), Expect = 4.48e-214
Identity = 285/395 (72.15%), Postives = 336/395 (85.06%), Query Frame = 0

Query: 3   FSVVVAVALLVLGCCCDGGHAGVVAPPEAFGICPSAVTIHGYKCQEIQVTTEDGYILNLQ 62
           FSVV+  A++VLG CC GGH GV+  PE  GIC SAVTIHGY CQEIQVTT+DGYIL+LQ
Sbjct: 7   FSVVMTAAVVVLGGCCGGGHGGVLGSPEELGICASAVTIHGYMCQEIQVTTKDGYILSLQ 66

Query: 63  RIPEGRRGGGPK-KKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVWITNTRGTR 122
           RIP  R G G   KK PVI+QHG+LVDG +WLLNSP+QNLP+ILAD GFDVWI+NTRGT+
Sbjct: 67  RIPHSRHGSGRGIKKPPVIVQHGILVDGMSWLLNSPEQNLPLILADNGFDVWISNTRGTQ 126

Query: 123 FSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGTLIILASLS 182
           FSRRHT+L+P  +++WNWSWD+L AY+LPAVFDHVSQ+TG+KIHYVGHSLGTLI+LASLS
Sbjct: 127 FSRRHTALNPADRDFWNWSWDELAAYELPAVFDHVSQQTGRKIHYVGHSLGTLIMLASLS 186

Query: 183 EGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFLK 242
           EGRLV++++SV LLSPIAYLSHMTTA+GALAA+S +GE+   LG+ EFNPKG+AV  F+K
Sbjct: 187 EGRLVNQVQSVGLLSPIAYLSHMTTAVGALAAQSLLGEIIESLGIIEFNPKGQAVEKFVK 246

Query: 243 VLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIARHGVLAKY 302
            LCA  G+NCYDLL A+TG NCCLNSSTV  FL NEPQSTSTKN+VHLAQI +HGV AKY
Sbjct: 247 YLCAFPGMNCYDLLPAITGDNCCLNSSTVQFFLDNEPQSTSTKNLVHLAQIVKHGVFAKY 306

Query: 303 NYGKIF-NLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHDVD 362
           NY  I+ NL HY +++PP+YN SNIPHD+ IFISYGGRDALSDV DV HLLDHFK HDVD
Sbjct: 307 NYDGIYLNLKHYGKIKPPVYNFSNIPHDLAIFISYGGRDALSDVEDVNHLLDHFKFHDVD 366

Query: 363 KFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDK 395
           K  VQFI  YAHAD++MG++A+D+VY  L+AFF K
Sbjct: 367 KLYVQFIQNYAHADYVMGINANDMVYKPLIAFFKK 401

BLAST of MC08g1219 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 494.6 bits (1272), Expect = 7.5e-140
Identity = 241/403 (59.80%), Postives = 299/403 (74.19%), Query Frame = 0

Query: 1   MAFSVVVAVALLVLGCCCDGGHAGVVAPP----EAFGICPSAVTIHGYKCQEIQVTTEDG 60
           +A SV++  AL +      G    +   P     A GIC S+V I GYKC+E  V T+DG
Sbjct: 14  LALSVLIFFALSLKTLEARGTFGRLAGQPPQRTAAGGICASSVHIFGYKCEEHDVVTQDG 73

Query: 61  YILNLQRIPEGRRG---GGPKKKQPVIIQHGVLVDGTTWLLNSPDQNLPMILADKGFDVW 120
           YILN+QRIPEGR G   G   K+QPV+IQHG+LVDG +WLLN  DQNLP+ILAD+GFDVW
Sbjct: 74  YILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGMSWLLNPADQNLPLILADQGFDVW 133

Query: 121 ITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFDHVSQETGQKIHYVGHSLGT 180
           + NTRGTRFSRRH  L+P  + +WNW+WD+LV+YDLPA+FDH+   TGQKIHY+GHSLGT
Sbjct: 134 MGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLPAMFDHIHGLTGQKIHYLGHSLGT 193

Query: 181 LIILASLSEGRLVDKLRSVALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKG 240
           LI  AS SE  LVD++RS A+LSP+AYLSHMTT IG +AA++F+ E T++LG  EFNPK 
Sbjct: 194 LIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPEFNPKS 253

Query: 241 EAVGHFLKVLCANQGINCYDLLTALTGHNCCLNSSTVGLFLMNEPQSTSTKNMVHLAQIA 300
             VG F+K +C   GI+CYDL++ +TG NCCLN+ST+ LFL NEPQSTSTKNM+HLAQ  
Sbjct: 254 GLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIHLAQTV 313

Query: 301 RHGVLAKYNYGKI-FNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLD 360
           R   L KYNYG    N+ HY +  PP YN+S IPH++P+F SYGG D+L+DV DV  LLD
Sbjct: 314 RDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLD 373

Query: 361 HFKLHDVDKFSVQFIDRYAHADFIMGVDAHDVVYSSLVAFFDK 396
            FK HD+DK +VQF+  YAHADFIMGV A DVVY+ +  FF +
Sbjct: 374 QFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQVATFFKR 416

BLAST of MC08g1219 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 271.9 bits (694), Expect = 7.9e-73
Identity = 136/362 (37.57%), Postives = 206/362 (56.91%), Query Frame = 0

Query: 34  ICPSAVTIHGYKCQEIQVTTEDGYILNLQRIPE--GRRGGGPKKKQPVIIQHGVLVDGTT 93
           +C   +    Y C E  + T+DGYIL LQR+     R   GP    PV++QHG+ + G  
Sbjct: 32  LCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQSGP----PVLLQHGLFMAGDV 91

Query: 94  WLLNSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAYDLPA 153
           W LNSP ++L  ILAD GFDVW+ N RGTR+S  H +L    KE+W+WSW  L  YDL  
Sbjct: 92  WFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYDLAE 151

Query: 154 VFDHVSQETGQKIHYVGHSLGTLIILASLSEGRLVDKLRSVALLSPIAYLSHMTTAIGAL 213
           +  ++   +  KI  VGHS GT++  A+L++  + + + + ALL PI+YL H+T  +   
Sbjct: 152 MIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPLVER 211

Query: 214 AARSFVGELTTLLGVAEFNPKGEAVGHFLKVLCANQGINCYDLLTALTGHNCCLNSSTVG 273
                + ++   LG+ + N + + +   +  LC    ++C D LT++TG NCC N+S + 
Sbjct: 212 MVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGH-MDCTDFLTSITGTNCCFNASKIE 271

Query: 274 LFLMNEPQSTSTKNMVHLAQIARHGVLAKYNYGKIFNLFHYKELRPPIYNLSNIPHDVPI 333
            +L  EP  +S KN+ HL Q+ R G  A+Y+YG   NL  Y   +PP + LS+IP  +P+
Sbjct: 272 YYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASLPM 331

Query: 334 FISYGGRDALSDVPDVTHLLDHFKLHDVDKFSVQFIDRYAHADFIMGVDAHDVVYSSLVA 393
           ++ YGG D L+DV DV H L            + +++ Y H DF++G  A + VY  ++ 
Sbjct: 332 WMGYGGTDGLADVTDVEHTLAELP----SSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQ 384

BLAST of MC08g1219 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 7.8e-20
Identity = 103/377 (27.32%), Postives = 159/377 (42.18%), Query Frame = 0

Query: 35  CPSAVTIHGYKCQEIQVTTEDGYILNLQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLL 94
           C   +T  GY  + I+V T DGY+L L+RIP  RR      ++ V +QHGVL     W+ 
Sbjct: 296 CQDVITELGYPYEAIRVITSDGYVLVLERIP--RRDA----RKAVFLQHGVLDSSMGWVS 355

Query: 95  NSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVF- 154
           N    +      D+G+DV++ N RG   SR H + +  SKE+W +S ++    D+PA+  
Sbjct: 356 NGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDIPAMIE 415

Query: 155 ------------------DHVSQETGQKIHYVGHSLGTLIILASLSEGRLVDK---LRSV 214
                             + ++QE   K+  + HSLG   IL  +   ++ +K   L  +
Sbjct: 416 KIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRLSRL 475

Query: 215 ALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFLKVLCANQGINCY 274
            LLSP  +  H  + +G          ++ +L  A   P       F ++L  N+    +
Sbjct: 476 ILLSPAGF--HEDSNLGFTIVEYIFLFISPVL--ARIVPAFYIPTRFFRML-LNKLARDF 535

Query: 275 DLLTALTGHNCCLNSSTVG-------------LFLMNEPQSTSTKNMVHLAQIARHGVLA 334
               AL G    L S  VG              + MN+  + S +   HLAQI   G   
Sbjct: 536 HNYPALGGLVQTLMSYVVGGDSSNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFR 595

Query: 335 KYNYG-KIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHD 376
            Y+YG +  N+  Y    P     S    DVP+ +  G  D +     V    +  +  +
Sbjct: 596 MYDYGSRSANMEVYGSPEPLDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAE 655

BLAST of MC08g1219 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 7.8e-20
Identity = 103/377 (27.32%), Postives = 159/377 (42.18%), Query Frame = 0

Query: 35  CPSAVTIHGYKCQEIQVTTEDGYILNLQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLL 94
           C   +T  GY  + I+V T DGY+L L+RIP  RR      ++ V +QHGVL     W+ 
Sbjct: 227 CQDVITELGYPYEAIRVITSDGYVLVLERIP--RRDA----RKAVFLQHGVLDSSMGWVS 286

Query: 95  NSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVF- 154
           N    +      D+G+DV++ N RG   SR H + +  SKE+W +S ++    D+PA+  
Sbjct: 287 NGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDIPAMIE 346

Query: 155 ------------------DHVSQETGQKIHYVGHSLGTLIILASLSEGRLVDK---LRSV 214
                             + ++QE   K+  + HSLG   IL  +   ++ +K   L  +
Sbjct: 347 KIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRLSRL 406

Query: 215 ALLSPIAYLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFLKVLCANQGINCY 274
            LLSP  +  H  + +G          ++ +L  A   P       F ++L  N+    +
Sbjct: 407 ILLSPAGF--HEDSNLGFTIVEYIFLFISPVL--ARIVPAFYIPTRFFRML-LNKLARDF 466

Query: 275 DLLTALTGHNCCLNSSTVG-------------LFLMNEPQSTSTKNMVHLAQIARHGVLA 334
               AL G    L S  VG              + MN+  + S +   HLAQI   G   
Sbjct: 467 HNYPALGGLVQTLMSYVVGGDSSNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFR 526

Query: 335 KYNYG-KIFNLFHYKELRPPIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDHFKLHD 376
            Y+YG +  N+  Y    P     S    DVP+ +  G  D +     V    +  +  +
Sbjct: 527 MYDYGSRSANMEVYGSPEPLDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAE 586

BLAST of MC08g1219 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 83.6 bits (205), Expect = 4.0e-16
Identity = 97/383 (25.33%), Postives = 163/383 (42.56%), Query Frame = 0

Query: 35  CPSAVTIHGYKCQEIQVTTEDGYILNLQRIPEGRRGGGPKKKQPVIIQHGVLVDGTTWLL 94
           C   +T  GY  + I+V T DGY L L+RIP  RR      ++ V +QHGV+     W+ 
Sbjct: 294 CQDVITELGYPYEAIRVVTSDGYGLLLERIP--RRDA----RKAVYLQHGVMDSSMGWVS 353

Query: 95  NSPDQNLPMILADKGFDVWITNTRGTRFSRRHTSLDPDSKEYWNWSWDQLVAYDLPAVFD 154
           N    +      D+G+DV++ N RG   SR H   +  SK++W +S ++    D+PA+ +
Sbjct: 354 NGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVKKNISSKDFWRYSINEHATEDIPAMIE 413

Query: 155 H-------------------VSQETGQKIHYVGHSLGTLIILASLSEGRLVDK---LRSV 214
                               V+++   K+  V HSLG   +L  +   ++ +K   L  +
Sbjct: 414 KIHEIKTSELKLYQPTMEEVVNEDQPYKLCVVSHSLGGAAVLMYVITRKIEEKPHRLSRL 473

Query: 215 ALLSPIA--YLSHMTTAIGALAARSFVGELTTLLGVAEFNPKGEAVGHFLKVLCANQGIN 274
            LLSP    Y S+M   +       F+G + + +  A + P       F ++L      +
Sbjct: 474 ILLSPAGFHYDSNMCFTLMEYTF-LFLGPVLSRIVPAFYIPT-----KFFRMLLNKLARD 533

Query: 275 CYD----------LLTALTGHNCCLNSSTVGL--FLMNEPQSTSTKNMVHLAQIARHGVL 334
            ++          L++ + G +       +GL  + MN+    S +   HLAQI   G  
Sbjct: 534 FHNYPAVGGLVQTLMSYVVGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQIKHSGKF 593

Query: 335 AKYNYG-KIFNLFHYKELRP----PIYNLSNIPHDVPIFISYGGRDALSDVPDVTHLLDH 376
             ++YG    N+  Y    P      Y L     DVP+ +  G +D    V   + +  H
Sbjct: 594 KMFDYGSSSANMDVYGSPEPLDLGEFYGLI----DVPVDLVAGKKD---KVIRPSMVRKH 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q67ZU11.1e-13859.80Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1[more]
Q71DJ51.1e-7137.57Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
P800358.5e-5635.31Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV... [more]
P070985.5e-5534.84Gastric triacylglycerol lipase OS=Homo sapiens OX=9606 GN=LIPF PE=1 SV=1[more]
Q3YBN29.5e-5533.95Tear acid lipase-like protein OS=Mesocricetus auratus OX=10036 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022154145.11.37e-297100.00triacylglycerol lipase 2 [Momordica charantia][more]
XP_008450088.12.45e-22075.57PREDICTED: triacylglycerol lipase 2 isoform X2 [Cucumis melo][more]
XP_008450087.11.71e-21875.38PREDICTED: triacylglycerol lipase 2 isoform X1 [Cucumis melo][more]
XP_011651565.19.04e-21674.31triacylglycerol lipase 2 isoform X2 [Cucumis sativus] >KGN58188.1 hypothetical p... [more]
XP_011651564.16.29e-21474.12triacylglycerol lipase 2 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1DIT26.63e-298100.00Lipase OS=Momordica charantia OX=3673 GN=LOC111021471 PE=3 SV=1[more]
A0A1S3BMV91.19e-22075.57Lipase OS=Cucumis melo OX=3656 GN=LOC103491778 PE=3 SV=1[more]
A0A1S3BPG08.27e-21975.38Lipase OS=Cucumis melo OX=3656 GN=LOC103491778 PE=3 SV=1[more]
A0A0A0L8N74.38e-21674.31Lipase OS=Cucumis sativus OX=3659 GN=Csa_3G588470 PE=3 SV=1[more]
A0A6J1F9944.48e-21472.15Lipase OS=Cucurbita moschata OX=3662 GN=LOC111441988 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G14180.17.5e-14059.80Myzus persicae-induced lipase 1 [more]
AT2G15230.17.9e-7337.57lipase 1 [more]
AT1G73920.17.8e-2027.32alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.27.8e-2027.32alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.14.0e-1625.33alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 39..95
e-value: 1.5E-17
score: 62.9
IPR025483Lipase, eukaryoticPIRSFPIRSF000862Steryl_ester_lipcoord: 1..396
e-value: 2.3E-126
score: 419.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 26..395
e-value: 6.2E-116
score: 389.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 39..394
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 101..228
e-value: 7.6E-11
score: 42.1
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 29..396
NoneNo IPR availablePANTHERPTHR11005:SF99TRIACYLGLYCEROL LIPASE 2coord: 29..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1219.1MC08g1219.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002213 defense response to insect
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds