MC08g1170 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1170 vs. NCBI nr
Match: XP_022148288.1 (uncharacterized protein LOC111016977 [Momordica charantia])

HSP 1 Score: 846 bits (2185), Expect = 3.92e-309
Identity = 429/433 (99.08%), Postives = 429/433 (99.08%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60
           MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP
Sbjct: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60

Query: 61  SSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120
           SSFFAGGRKLNHRNSFGFRD FEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI
Sbjct: 61  SSFFAGGRKLNHRNSFGFRDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120

Query: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA 180
           GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA
Sbjct: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA 180

Query: 181 NHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDVKLQK 240
           NHLAAKVRLKEDAEASKTTDSLPISENVALPIA PECNASPNPKSILKRKDNHIDVK QK
Sbjct: 181 NHLAAKVRLKEDAEASKTTDSLPISENVALPIAVPECNASPNPKSILKRKDNHIDVKSQK 240

Query: 241 RVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH 300
           RVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH
Sbjct: 241 RVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH 300

Query: 301 YTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI 360
           YTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI
Sbjct: 301 YTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI 360

Query: 361 ENSALGSQNDQNGAGKEVAQQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY 420
           ENSALGSQNDQNGAGKEV QQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY
Sbjct: 361 ENSALGSQNDQNGAGKEVVQQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY 420

Query: 421 RMRAKMESEEEEA 433
           RMRAKMESEEEEA
Sbjct: 421 RMRAKMESEEEEA 433

BLAST of MC08g1170 vs. NCBI nr
Match: XP_023531936.1 (uncharacterized protein LOC111794053 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 631 bits (1628), Expect = 3.19e-224
Identity = 333/438 (76.03%), Postives = 368/438 (84.02%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTS----TAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEP 60
           MED+FKVRVDRIFGSLSSSS+S    TAA NS LSSLW LTDDE+ER+EWIK K D PEP
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPEP 60

Query: 61  EPEPSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEI 120
           E EP+SF AG RK+N RNSF FRD FEDD+  DLDE+PESNGSSS FPKPDDYGDEEWEI
Sbjct: 61  ELEPNSFLAGERKVNGRNSFRFRDDFEDDV-GDLDEDPESNGSSSNFPKPDDYGDEEWEI 120

Query: 121 KSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTR 180
           KS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS+RNFTR
Sbjct: 121 KSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIRNFTR 180

Query: 181 DPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDV 240
           DPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECNAS NPKSILKRKDN +D 
Sbjct: 181 DPRANHLAAKVRLKEDAEASKTMDSLHVSENGAVAIAVSECNASHNPKSILKRKDNPLDA 240

Query: 241 KLQKRVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPS 300
           K  KRVRF+PEC+IS++ +GS+DISME NSS   A V  EATF S G+R Q PDY++NPS
Sbjct: 241 KSHKRVRFDPECEISREPQGSEDISMEANSSIGAAEVTTEATFQSQGFRPQAPDYLRNPS 300

Query: 301 RYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTME-SHEDDASAGPPKYITFIPKKKAG 360
           RYTHYTFDSS +VD+E N+ AY+DFLQLVRGSKT+E SH DD S GPPK ITFIPKKKAG
Sbjct: 301 RYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPSHLDDFSTGPPKSITFIPKKKAG 360

Query: 361 DTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRNS 420
           DTVM+EN   G QN QNG GKE+  Q+G+ IGI   D QTD+VCSMEEDEP+KLE RRNS
Sbjct: 361 DTVMLENPTPGEQNHQNGVGKELVHQKGMPIGIASVDAQTDDVCSMEEDEPEKLETRRNS 420

Query: 421 SQKPGRQYRMRAKMESEE 430
           SQK  RQYRMRAKM+SEE
Sbjct: 421 SQKTARQYRMRAKMDSEE 437

BLAST of MC08g1170 vs. NCBI nr
Match: XP_022928536.1 (uncharacterized protein LOC111435316 [Cucurbita moschata])

HSP 1 Score: 630 bits (1626), Expect = 6.43e-224
Identity = 333/439 (75.85%), Postives = 369/439 (84.05%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTS-----TAALNSSLSSLWSLTDDEIERKEWIKEKEDPPE 60
           MED+FKVRVDRIFGSLSSSS+S     TAA NS LSSLW LTDDE+ER+EWIK K D PE
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPE 60

Query: 61  PEPEPSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWE 120
           PE EP+SF AG RK++ RNSF FRD FEDD+  DLDENPESNGSSS FPKPDDYGDEEWE
Sbjct: 61  PELEPTSFLAGERKVSGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEWE 120

Query: 121 IKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFT 180
           IKS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS++NFT
Sbjct: 121 IKSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIQNFT 180

Query: 181 RDPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHID 240
           RDPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECNAS NPKSILKRKDNH+D
Sbjct: 181 RDPRANHLAAKVRLKEDAEASKTMDSLHVSENGAVAIAVSECNASQNPKSILKRKDNHLD 240

Query: 241 VKLQKRVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNP 300
            K  KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+R Q PDY++NP
Sbjct: 241 AKSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFHSQGFRPQAPDYLRNP 300

Query: 301 SRYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKKA 360
           SRYTHYTFDSS +VD+E N+ AY+DFLQLVRGSKT+E  H DD S GPPK ITFIPKKKA
Sbjct: 301 SRYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDTSTGPPKSITFIPKKKA 360

Query: 361 GDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRN 420
           GDTVM+EN   G QN QNG GKE+  Q+G+ IGI   D QTD+VCSMEEDEP+KLE RRN
Sbjct: 361 GDTVMLENPTPGEQNHQNGVGKEL-HQKGMPIGIASVDAQTDDVCSMEEDEPEKLETRRN 420

Query: 421 SSQKPGRQYRMRAKMESEE 430
           SSQK  RQYRMRAKM+SEE
Sbjct: 421 SSQKTARQYRMRAKMDSEE 437

BLAST of MC08g1170 vs. NCBI nr
Match: XP_022989648.1 (uncharacterized protein LOC111486668 [Cucurbita maxima])

HSP 1 Score: 630 bits (1626), Expect = 6.43e-224
Identity = 331/438 (75.57%), Postives = 366/438 (83.56%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTS----TAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEP 60
           MED+FKVRVDRIFGSLSSSS+S    TAA NS LSSLW LTDDE+ER+EWIK K D PEP
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPEP 60

Query: 61  EPEPSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEI 120
           E EP+SF AG RK+N RNSF FRD FEDD+  DLDENPESNGSSS FPKPDDYGDEEWEI
Sbjct: 61  ELEPTSFLAGERKVNGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEWEI 120

Query: 121 KSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTR 180
           KS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS+RNFTR
Sbjct: 121 KSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIRNFTR 180

Query: 181 DPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDV 240
           DPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECN S NPKSILKRKDNH+D 
Sbjct: 181 DPRANHLAAKVRLKEDAEASKTMDSLHVSENDAVAIAVSECNVSQNPKSILKRKDNHLDA 240

Query: 241 KLQKRVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPS 300
           K  KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+  Q PDY++NPS
Sbjct: 241 KSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFQSQGFCRQAPDYLRNPS 300

Query: 301 RYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKKAG 360
           RYTHYTFDSS +VD+E N+ AY+DFLQLVRGSKT+E  H DD+S GPPK ITFIPKKKAG
Sbjct: 301 RYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDSSTGPPKSITFIPKKKAG 360

Query: 361 DTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRNS 420
           DTVM+EN   G QN QNG GKE+  Q+G+ I I   D QTD+VCSMEEDEP+KLE RRN 
Sbjct: 361 DTVMLENPTPGEQNHQNGVGKELVHQKGMPISIASVDAQTDDVCSMEEDEPEKLETRRNI 420

Query: 421 SQKPGRQYRMRAKMESEE 430
           SQK  RQYRMRAKM+SEE
Sbjct: 421 SQKTARQYRMRAKMDSEE 437

BLAST of MC08g1170 vs. NCBI nr
Match: KAG6588861.1 (hypothetical protein SDJN03_17426, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022621.1 hypothetical protein SDJN02_16355, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629 bits (1621), Expect = 3.85e-223
Identity = 333/440 (75.68%), Postives = 368/440 (83.64%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTS------TAALNSSLSSLWSLTDDEIERKEWIKEKEDPP 60
           MED+FKVRVDRIFGSLSSSS+S      TAA NS LSSLW LTDDE+ER+EWIK K D P
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQP 60

Query: 61  EPEPEPSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEW 120
           EPE EP+SF AG RK++ RNSF FRD FEDD+  DLDENPESNGSSS FPKPDDYGDEEW
Sbjct: 61  EPELEPTSFLAGERKVSGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEW 120

Query: 121 EIKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNF 180
           EIKS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS+RNF
Sbjct: 121 EIKSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIRNF 180

Query: 181 TRDPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHI 240
           TRDPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECNAS NPKSILKRKDNH+
Sbjct: 181 TRDPRANHLAAKVRLKEDAEASKTMDSLHVSENGAVAIAVSECNASQNPKSILKRKDNHL 240

Query: 241 DVKLQKRVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQN 300
           D K  KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+  Q PDY++N
Sbjct: 241 DAKSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFHSQGFCPQAPDYLRN 300

Query: 301 PSRYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKK 360
           PSRYTHYTFDSS +VD+E N+ AY+DFLQLVRGSKT+E  H DD S GPPK ITFIPKKK
Sbjct: 301 PSRYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDTSTGPPKSITFIPKKK 360

Query: 361 AGDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRR 420
           AGDTVM+EN   G QN QNG GKE+  Q+G+ IGI   D QTD+VCSMEEDEP+KLE RR
Sbjct: 361 AGDTVMLENPTPGEQNHQNGVGKEL-HQKGMPIGIASVDAQTDDVCSMEEDEPEKLETRR 420

Query: 421 NSSQKPGRQYRMRAKMESEE 430
           NSSQK  RQYRMRAKM+SEE
Sbjct: 421 NSSQKTARQYRMRAKMDSEE 438

BLAST of MC08g1170 vs. ExPASy TrEMBL
Match: A0A6J1D3P7 (Protein TSSC4 OS=Momordica charantia OX=3673 GN=LOC111016977 PE=3 SV=1)

HSP 1 Score: 846 bits (2185), Expect = 1.90e-309
Identity = 429/433 (99.08%), Postives = 429/433 (99.08%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60
           MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP
Sbjct: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60

Query: 61  SSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120
           SSFFAGGRKLNHRNSFGFRD FEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI
Sbjct: 61  SSFFAGGRKLNHRNSFGFRDGFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120

Query: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA 180
           GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA
Sbjct: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPRA 180

Query: 181 NHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDVKLQK 240
           NHLAAKVRLKEDAEASKTTDSLPISENVALPIA PECNASPNPKSILKRKDNHIDVK QK
Sbjct: 181 NHLAAKVRLKEDAEASKTTDSLPISENVALPIAVPECNASPNPKSILKRKDNHIDVKSQK 240

Query: 241 RVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH 300
           RVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH
Sbjct: 241 RVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYTH 300

Query: 301 YTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI 360
           YTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI
Sbjct: 301 YTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVMI 360

Query: 361 ENSALGSQNDQNGAGKEVAQQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY 420
           ENSALGSQNDQNGAGKEV QQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY
Sbjct: 361 ENSALGSQNDQNGAGKEVVQQRGIAIGIDNQTDEVCSMEEDEPDKLELRRNSSQKPGRQY 420

Query: 421 RMRAKMESEEEEA 433
           RMRAKMESEEEEA
Sbjct: 421 RMRAKMESEEEEA 433

BLAST of MC08g1170 vs. ExPASy TrEMBL
Match: A0A6J1JQY5 (Protein TSSC4 OS=Cucurbita maxima OX=3661 GN=LOC111486668 PE=3 SV=1)

HSP 1 Score: 630 bits (1626), Expect = 3.11e-224
Identity = 331/438 (75.57%), Postives = 366/438 (83.56%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTS----TAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEP 60
           MED+FKVRVDRIFGSLSSSS+S    TAA NS LSSLW LTDDE+ER+EWIK K D PEP
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPEP 60

Query: 61  EPEPSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEI 120
           E EP+SF AG RK+N RNSF FRD FEDD+  DLDENPESNGSSS FPKPDDYGDEEWEI
Sbjct: 61  ELEPTSFLAGERKVNGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEWEI 120

Query: 121 KSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTR 180
           KS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS+RNFTR
Sbjct: 121 KSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIRNFTR 180

Query: 181 DPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDV 240
           DPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECN S NPKSILKRKDNH+D 
Sbjct: 181 DPRANHLAAKVRLKEDAEASKTMDSLHVSENDAVAIAVSECNVSQNPKSILKRKDNHLDA 240

Query: 241 KLQKRVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPS 300
           K  KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+  Q PDY++NPS
Sbjct: 241 KSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFQSQGFCRQAPDYLRNPS 300

Query: 301 RYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKKAG 360
           RYTHYTFDSS +VD+E N+ AY+DFLQLVRGSKT+E  H DD+S GPPK ITFIPKKKAG
Sbjct: 301 RYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDSSTGPPKSITFIPKKKAG 360

Query: 361 DTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRNS 420
           DTVM+EN   G QN QNG GKE+  Q+G+ I I   D QTD+VCSMEEDEP+KLE RRN 
Sbjct: 361 DTVMLENPTPGEQNHQNGVGKELVHQKGMPISIASVDAQTDDVCSMEEDEPEKLETRRNI 420

Query: 421 SQKPGRQYRMRAKMESEE 430
           SQK  RQYRMRAKM+SEE
Sbjct: 421 SQKTARQYRMRAKMDSEE 437

BLAST of MC08g1170 vs. ExPASy TrEMBL
Match: A0A6J1EKJ9 (Protein TSSC4 OS=Cucurbita moschata OX=3662 GN=LOC111435316 PE=3 SV=1)

HSP 1 Score: 630 bits (1626), Expect = 3.11e-224
Identity = 333/439 (75.85%), Postives = 369/439 (84.05%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTS-----TAALNSSLSSLWSLTDDEIERKEWIKEKEDPPE 60
           MED+FKVRVDRIFGSLSSSS+S     TAA NS LSSLW LTDDE+ER+EWIK K D PE
Sbjct: 1   MEDNFKVRVDRIFGSLSSSSSSSSSYSTAAFNSPLSSLWCLTDDEVERREWIKGKVDQPE 60

Query: 61  PEPEPSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWE 120
           PE EP+SF AG RK++ RNSF FRD FEDD+  DLDENPESNGSSS FPKPDDYGDEEWE
Sbjct: 61  PELEPTSFLAGERKVSGRNSFRFRDDFEDDV-GDLDENPESNGSSSNFPKPDDYGDEEWE 120

Query: 121 IKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFT 180
           IKS IGRDCTLD+EEEED YDKVAVGKEK GDRLYMKDI DCG+EIDSCTELPTS++NFT
Sbjct: 121 IKSLIGRDCTLDFEEEEDEYDKVAVGKEKVGDRLYMKDITDCGVEIDSCTELPTSIQNFT 180

Query: 181 RDPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHID 240
           RDPRANHLAAKVRLKEDAEASKT DSL +SEN A+ IA  ECNAS NPKSILKRKDNH+D
Sbjct: 181 RDPRANHLAAKVRLKEDAEASKTMDSLHVSENGAVAIAVSECNASQNPKSILKRKDNHLD 240

Query: 241 VKLQKRVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNP 300
            K  KRVRF+PEC+IS++ +GS+DISME NSS   A V NEATF S G+R Q PDY++NP
Sbjct: 241 AKSHKRVRFDPECEISRESQGSEDISMEANSSIGAAEVTNEATFHSQGFRPQAPDYLRNP 300

Query: 301 SRYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMES-HEDDASAGPPKYITFIPKKKA 360
           SRYTHYTFDSS +VD+E N+ AY+DFLQLVRGSKT+E  H DD S GPPK ITFIPKKKA
Sbjct: 301 SRYTHYTFDSSNEVDEESNKNAYMDFLQLVRGSKTIEPLHLDDTSTGPPKSITFIPKKKA 360

Query: 361 GDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRN 420
           GDTVM+EN   G QN QNG GKE+  Q+G+ IGI   D QTD+VCSMEEDEP+KLE RRN
Sbjct: 361 GDTVMLENPTPGEQNHQNGVGKEL-HQKGMPIGIASVDAQTDDVCSMEEDEPEKLETRRN 420

Query: 421 SSQKPGRQYRMRAKMESEE 430
           SSQK  RQYRMRAKM+SEE
Sbjct: 421 SSQKTARQYRMRAKMDSEE 437

BLAST of MC08g1170 vs. ExPASy TrEMBL
Match: A0A6J1IZZ9 (Protein TSSC4 OS=Cucurbita maxima OX=3661 GN=LOC111481475 PE=3 SV=1)

HSP 1 Score: 615 bits (1586), Expect = 3.27e-218
Identity = 324/436 (74.31%), Postives = 368/436 (84.40%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTST-AALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPE 60
           M+DSFKVRVDRIFGSLSS+STS+ AA NSS+ SLW LTDDEIER+EWIKEKE+  EPE E
Sbjct: 1   MDDSFKVRVDRIFGSLSSTSTSSPAAFNSSVRSLWCLTDDEIERREWIKEKEEQLEPELE 60

Query: 61  PSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSS 120
           P+SFF GG+K++ R+SFGF+D FEDD   DLDENP SNGSSSKFPKPDDYGDEEWEIKSS
Sbjct: 61  PTSFFDGGKKVSGRSSFGFQDDFEDDELDDLDENPGSNGSSSKFPKPDDYGDEEWEIKSS 120

Query: 121 IGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNFTRDPR 180
           IGRDCTLDYEEEED YDKVAVGKEK GDRLYMKD  DCGIEI SCTELPTS+RNFTRDPR
Sbjct: 121 IGRDCTLDYEEEEDEYDKVAVGKEKGGDRLYMKDATDCGIEIGSCTELPTSVRNFTRDPR 180

Query: 181 ANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHIDVKLQ 240
           ANHLAAKVRLKEDAEASKT DSL +S N  + IAD ECNAS NPKSILKRKDN++D KL 
Sbjct: 181 ANHLAAKVRLKEDAEASKTIDSLHVSVNSTVAIADSECNASQNPKSILKRKDNYLDAKLH 240

Query: 241 KRVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQNPSRYT 300
           KRVRF+PECK SQD +G KD +M+ NS P  A V NEA + S    TQVPDY++NPSRYT
Sbjct: 241 KRVRFDPECKASQDSQGFKDFAMQANSLPGVAEVGNEANYPSQA--TQVPDYLRNPSRYT 300

Query: 301 HYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKAGDTVM 360
           HYTFDSS +VD+E N+KAY+DFLQLVR SKTMES++DD S GPP+ ITFIPKKKAGDT+M
Sbjct: 301 HYTFDSSKEVDEESNKKAYMDFLQLVRRSKTMESNQDDDSTGPPRSITFIPKKKAGDTIM 360

Query: 361 IENSALGSQND-QNGAGKEVAQQRGIAIGID---NQTDEVCSMEEDEPDKLELRRNSSQK 420
           +E+S+L  Q+  QNG GKE+  Q+G+ IGI     Q D+VCSMEEDEPD+LE+R+NS QK
Sbjct: 361 LESSSLEEQSHHQNGVGKELVHQKGMPIGIAAVYTQNDDVCSMEEDEPDRLEIRKNSLQK 420

Query: 421 PGRQYRMRAKMESEEE 431
            GRQYRMRA +ESEEE
Sbjct: 421 SGRQYRMRA-LESEEE 433

BLAST of MC08g1170 vs. ExPASy TrEMBL
Match: A0A0A0LDS6 (Protein TSSC4 OS=Cucumis sativus OX=3659 GN=Csa_3G599460 PE=3 SV=1)

HSP 1 Score: 608 bits (1569), Expect = 1.26e-215
Identity = 329/441 (74.60%), Postives = 366/441 (82.99%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSST----STAALNSSLSSLWSLTDDEIERKEWIK--EKEDPP 60
           M+DSFKVRVDRIFGSLSSSST    S++  NSSLSSLWSLTDDEIER+EWIK  E+E PP
Sbjct: 1   MDDSFKVRVDRIFGSLSSSSTTSSSSSSPFNSSLSSLWSLTDDEIERREWIKGKEEEQPP 60

Query: 61  EPEPEPSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEW 120
           E +  P+SFF G RK+N RNSFGFRD FEDDL+ D+DENPESNGSSSKFPKPDDYGDEEW
Sbjct: 61  ESDLAPTSFFDGLRKVNERNSFGFRDDFEDDLD-DVDENPESNGSSSKFPKPDDYGDEEW 120

Query: 121 EIKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLRNF 180
           EIKSSIGRDCTLDYEEEED YDKVAVG+EK+GDRLYMK+I+DCGIEI S TELPTS+RNF
Sbjct: 121 EIKSSIGRDCTLDYEEEEDEYDKVAVGREKNGDRLYMKEISDCGIEIGSSTELPTSIRNF 180

Query: 181 TRDPRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASPNPKSILKRKDNHI 240
           TRDPRANHLAAKVRLKEDAEASKT   L +SEN A+ I D E N S NPKSILKRKDNH+
Sbjct: 181 TRDPRANHLAAKVRLKEDAEASKTIHLLHVSENSAVAITDSESNTSQNPKSILKRKDNHL 240

Query: 241 DVKLQKRVRFNPECKISQDFRGSKDISMEGNSSPEDAYVCNEATFSSAGYRTQVPDYIQN 300
           D KL KRVRF+PECKI+Q  +GSKD   E NS P  A V NE TF S    TQVPDY+QN
Sbjct: 241 DAKLHKRVRFDPECKITQISQGSKDFVTETNSLPGAAEVGNEVTFPSRA--TQVPDYLQN 300

Query: 301 PSRYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYITFIPKKKA 360
           PSRYTHYTFDSS DVD+E N+KAY++FLQLVR SK +ESHE+DA  GPPK ITFIPKKKA
Sbjct: 301 PSRYTHYTFDSSNDVDEESNKKAYMNFLQLVRESKRIESHENDAPTGPPKSITFIPKKKA 360

Query: 361 GDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGI---DNQTDEVCSMEEDEPDKLELRRN 420
            DT+M+ENS       QNG GKEV  QRG++IGI   D+QTD+VCSMEEDEPDKLE + N
Sbjct: 361 DDTIMLENSPR-----QNGVGKEVVHQRGMSIGIATVDDQTDDVCSMEEDEPDKLETKTN 420

Query: 421 SSQKPGRQYRMRAK-MESEEE 431
           SSQKPGR+YR+RA  MESEEE
Sbjct: 421 SSQKPGRRYRIRANNMESEEE 433

BLAST of MC08g1170 vs. TAIR 10
Match: AT5G13970.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G13310.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 229.6 bits (584), Expect = 4.9e-60
Identity = 181/462 (39.18%), Postives = 244/462 (52.81%), Query Frame = 0

Query: 1   MEDSFKVRVDRIFGSLSSSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKEDPPEPEPEP 60
           M++SF+VR+D++FGSL+SSSTS+A     +SSLW L +DEI+  +   EKE       E 
Sbjct: 1   MDESFRVRIDKVFGSLASSSTSSA----PVSSLWCLAEDEIDGNQRSGEKE-----LSES 60

Query: 61  SSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDEEWEIKSSI 120
            + F+   K  H +         DDL  D     E  G SS+  KP DY DEEWEIK+SI
Sbjct: 61  LNSFSDNGKQEHES---------DDLSID-----EEKGRSSELQKPSDYDDEEWEIKNSI 120

Query: 121 GRDCTLDYEEEEDVYDKVAVGKEKSGDRLY--MKDI-ADCGIEIDSCTELPTSLRNFTRD 180
           G D TLD EEE D  DKVA+     G+++Y  MKD+  D   E D   ELP S     +D
Sbjct: 121 GMDSTLDMEEELDDNDKVAL-----GEKVYCCMKDVNDDYETEADEWVELPASFNEREKD 180

Query: 181 PRANHLAAKVRLKEDAEASKTTDSLPISENVALPIADPECNASP---------------- 240
           PRAN +AAK+RLKEDAEA    +SL +SE +   ++    N  P                
Sbjct: 181 PRANLIAAKLRLKEDAEAVNKLNSLHVSEELQDNLSMSTENEKPFVVSEDNLLGAFKESH 240

Query: 241 -------NPKSILKRKDNHI-DVKLQKRVRFNPECKISQDFRGSKDISMEGNSSPEDAYV 300
                    K ILKR++N   D K  KRVRF+ + K      G  D  ME +S  ED   
Sbjct: 241 VGSSDENGLKPILKRRENQADDSKSPKRVRFSSDVKDRTLTEGDNDSVMEASSPNEDKV- 300

Query: 301 CNEATFSSAGYRTQVPDYIQNPSRYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMES 360
                   A Y T +PDY++NPS+YT YTF+S  +VD+E NRKAY+DFL ++R       
Sbjct: 301 -------EAVYPTGIPDYMRNPSKYTRYTFESG-EVDEESNRKAYMDFLNMIRS------ 360

Query: 361 HEDDASAGP----PKYITFIPKKKAGDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGID 420
            +D++   P    P+ + F+PK+K     M E+       D  G        R +AI +D
Sbjct: 361 -KDESLVDPLMELPRSVAFVPKRKP----MAESKVENIDKDCEG--------RRVAIAVD 403

Query: 421 NQTD-EVCSMEEDEPDKLELRRNSSQKPGRQYRMRAKMESEE 431
              D  + +MEEDEP   E  ++ +++PGRQYR RAK + EE
Sbjct: 421 TIEDCTISAMEEDEP---ETAQHVTKRPGRQYRARAKEDPEE 403

BLAST of MC08g1170 vs. TAIR 10
Match: AT5G13310.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 7 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G13970.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 122.9 bits (307), Expect = 6.5e-28
Identity = 141/444 (31.76%), Postives = 202/444 (45.50%), Query Frame = 0

Query: 2   EDSFKVRVDRIFGSLS---------SSSTSTAALNSSLSSLWSLTDDEIERKEWIKEKED 61
           ++ F+ RV +IFGSL          S+S+S  A      S+W+L+D E+E++EW ++  D
Sbjct: 8   DEDFQSRVAKIFGSLPFSRPSSSKFSTSSSAPASRQQSGSVWTLSDTEVEKREWKRDSYD 67

Query: 62  PPEPEPEPSSFFAGGRKLNHRNSFGFRDVFEDDLEADLDENPESNGSSSKFPKPDDYGDE 121
             E  P  SSF      L  +         EDDL+ D+D   + +G              
Sbjct: 68  RDE-IPCASSF---DELLKQQKP------SEDDLK-DIDCGEDFDG-------------- 127

Query: 122 EWEIKSSIGRDCTLDYEEEEDVYDKVAVGKEKSGDRLYMKDIADCGIEIDSCTELPTSLR 181
            W I++S+G D TLD E EED YDKVA+G+E  G+         CG              
Sbjct: 128 VWSIRASMGLDRTLDDEAEEDEYDKVALGEENDGE--------GCG-------------- 187

Query: 182 NFTRDPRANHLAAKVRLKED-AEASKTTDSLPISENVALPIADPECNASPNPKSILKRKD 241
              RDPRAN++AA++RLKED  EA+K   S         P A+    A P  K ILKRK+
Sbjct: 188 -RIRDPRANYVAARIRLKEDEIEANKFNTSASQPSESKEPHAEESSEAMPR-KPILKRKE 247

Query: 242 NHID--VKLQKRVRFNPECKISQDFRGSKDISMEGNSSPED---AYVCNEATFSSAGYRT 301
           N  D   +  KRVRF+              +  E    PED   A   ++          
Sbjct: 248 NSSDSEARTSKRVRFD-------------SVPEETLKKPEDTCSASASSKIVSHQGKSGA 307

Query: 302 QVPDYIQNPSRYTHYTFDSSYDVDDEFNRKAYLDFLQLVRGSKTMESHEDDASAGPPKYI 361
           +VPDY+ NPS YT Y+FD S ++D E     Y+D    V G K  ES         PK +
Sbjct: 308 RVPDYLLNPSSYTRYSFDPSCELDVESPTGEYMDTPNAVEGLKNPESESF------PK-V 367

Query: 362 TFIPKKKAGDTVMIENSALGSQNDQNGAGKEVAQQRGIAIGIDNQTDEVCSMEEDEPDKL 421
           +FIP+ K  D  + E+S+  ++     AG E+A++   +   D  T+    + E E    
Sbjct: 368 SFIPQNKTKD--VSEDSSNCNETKPIVAG-ELAEEERPSATEDGDTE----IRESE---- 369

Query: 422 ELRRNSSQKPGRQYRMRAKMESEE 431
                S Q  GRQYR +  ++  +
Sbjct: 428 --SCTSFQSKGRQYRAKLSLDETD 369