MC08g1160 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1160
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein of unknown function (DUF803)
LocationMC08: 9816113 .. 9821542 (-)
RNA-Seq ExpressionMC08g1160
SyntenyMC08g1160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTATTCTCTCCGTTCCCAAATTGACCGCTCAACTTCTCCTCTCATTCACTTTTTTCTTTAATTAATTCATTATTATTTCATCTTCTCCCTTCTCCCCTCTCTGCAATCTACTCCGCTTCCCCATTTCTCTCTCCTTCTCCGGCGTCCGACGCTTTTCTCCACATTTCGGATTCCCGTGGGAGGGAACAAGCAAAGCAACTCCATCCCAATTCCAAGCCCGCGATTTCCCAATGCCCCACTTCCCAGATTCCATCTTCATCTTCTCAACTGCGCCCCATCTGATTCTGCACTGACCCATCTTCCCCACAGGTAATCTCTTTCCTTTCTGCTTGATTCTTTACATTTAGGAATTAGATCCGTAGTTGTTTCTTCCTCAGATCTTGATCACCAACTACCCTTTTCATTTCTTGTCAGCTTTACGAGTAATTGGCATTGTCTCTCACTTTCATCATTTGGTTGTCTACTTCTGTGTTGCTTTCAGCCGCAGGCTGGGATCCCAAGCTGTATGCAGATTCGCTCACACGTCTTTAATTTATGGGGGATTGGGTTATTGGAGCTTTCATCAACCTCTTTGGCAGCATTGCCATCAACTTTGGGACCAACCTTCTTAAATTAGGCCATAACGAGGTTCTAACAGATTTCTCTTTTTCTCATTCAATCTTGGTCTACACTGTGACTGAACAAACCGCTCTTCGCCTTCTTTTTATTCCACATTTTTCTTGAGGATACAATAGTCAATGTGTCATGCATCATGAAACATGAATGTCATCTTTTTCTTCCAAAAGATAAAATTCCACTTCAAAGTTCAAAGCTATAAACTTGTTACACTAATGGTTTGGTAAGTAATAGCCCATTAAACATCTAAGTTGAATGTTTAGTGGGACTCAATTCGGTCGCATTTTTCCATACTTCTGGGTTTCGAATATTCTTTTTGGCATTGTTTCATGCCGCAACGTTGTTACTTGTCACATGTCCTGTGGTTTTTAAAGAATATACTTTTGCAGAGAGAGAAGCATTCTATGCTAGAAAATAATGGCCCTAATGGAAAGACACCAATTAAGCCCATTATATACTTCCAGACTTGGAGAGTTGGTAAGTGATTTTCTGGTGTTAAATTCCTTTTATGGCTTTATGTGTTATACCTTTTTTGGAAGAAGCCTACATTTCAAACCTAAAGCCTTATTTATAAGTTTTGGCGTGCAGGTATTACATTTTTCATTCTTGGAAATTGCCTTAATTTTATTTCCTTTGGATATGCTGCCCAGGTTGGTAGAAATTACTTTATCCCATTTCTTAATATGATTATATTGGTATTATAAGTTCATCTATGTGGATTTTTTAAATTCTTTACAACTTTTACTGGATGTTTTTATTTTCTATTTATTGTGGATTTCCTGTTCCACCTTTTTTTAATTCCCGTTGTCGTCCCCCTTCTCCCCACCCCTACCCTGCAGTCTCTTCTTGCGGCACTTGGATCTGTACAGTTTGTGTCCAATATTGCTTTTGCATACTTCGTCCTCCACAAGATGGTTACTGTTAAGTATGTAAATCTCTGTAGTGGAACGTCTTCAAAACCATGAACATGAAGGCACATAACATTTTTTCATTATTTTTCTTCCTAATTTAATGGTTCATGACTATATGGCTTCTTTTGCAGGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAACATTTTTCTAGTTGCTTTTGGCAACCACCAGTCCCCTGGTAGATGCCATGCTTTTGATTTATTTATCTTTTAAGTTCTAATGGCTTGTCCTTCTATATGAAATGTCAAGAATGTCATTTGAGTTTTCCACGAAGTTTGTAATGATGAGGGGAAATGATCACTTAATAGACATCTCAAAACACCATCTGTTAAATTGCTAGAAATCAGCTGCCCAATATCTTGCAACCTGCATTATGATGGTGGTATATTTTTGTTCAGCACTTGTGACTATTCAGAATCACTTATCCTCTGGAAAAAATGTAATTAAGATTGCGTCAACAATTGATGTTGGCTAGAATTTAGAAATTAAACAAATAATGAATGCCTAATTTTTGCCATAGTTGATGCCTTCTACTTTTCCATTGCTATGAATAAAAAGATGACATATTTTATCTTTTGACTAAATATGTTGATTTTGGTAGATTAATGTTCACATTTCAGTTTGTTTAATGAGATCAATGCATTTAGTATGCCAAAGTTTACTGACTGATAAGATGCACGTACTGCATTTTCTTTAAGCAACGATACCTTTGACTCAATGCTAAGTAGTCTTTCATATGCATATTCCTTTGTCGTTATGCTGTGTGATTTAGATCCCAATTCTATGTGAAGATATTCAAGTGCCATGTAGTTAGGTTGTATAATGAGGCGGTGGTTTAACATAGAGAACTGGTTGTTGCTAATAACATTTTATTTTGCATTGAAGCCTACAAGTGTTAACCTCAATATCTAGTAGATTACTAACCAAAGAGGATTTCTTTCTGTCCACTACTAAGATGAGAAAAATTAAGGGGAAAGGGCATAAGAACAGGATGGTGGTGCAGAAAACATTTGATGCAATGAATTATTTGATCATAATCTGCTTGTAAATAATGAATGTTAATTTTTAGTCTTCTCTTTTCAGTGTACACTCCAGAACAGCTGGTGGAGAAATATGGAAATATAACTTTTCTTCTATACTGCGTAATTTTGATCTTGATTGTTGCTTTACATCACTCAATCTATAGGTTAGAATTTAATTGAACTCTTTTCTTCTTACTTTATATGAGGATTTTCATTTTGACCAATAGTTTCCCCTATGGAACATGCAGAAGGGGAGAATTGTTACTTTCTGATTCTGGACAAGATCTTAGACCGTATTGGCATATGCTGCTACCATTTTCCTATGCTATAGTTTCAGGTGCAATAGGCTCCTGCTCAGTCTTGTTTGCAAAATCTCTGTAAGTTCCCTGACTACTTTGTCCTTTTTTCTTGTTGGTACTGAACCCACTTGAATTTCTTGTTTTCATTCTCTTTCTAATGTCATGCTATCTGTTTTAGCTCAATACTGCTTAGATTGGCCATGTCTAGTGCTTACGAGCTGCATAGCTGGTTTACATACTCCTTGCTGCTTTTATTTCTCAGTACTGCTGGATTTTGGGTAAGTTCACAAATCACTATAGGATTAAGAACTCTACTTTTCCTTCTTTCATGTTTTTATGTTATTCTAGTGATGCGAAAAAACATCATTTCGTTAACCTTTAAGTCCATTATGAATTTCTTATCTATTTCCGCAGATGGCCAGGTTAAACGAAGGACTTTCCCTGTTTGATGCAATCCTTATTGTTCCCATGTTTCAGATTGCTTGGACTTTCTTCTCCATATGTACAGGTTTTATATACTTTAAAGAATTCCAGGTTCTCTCTCTCTCCCTCTCTCTCCGTGTGTGTGTGGCTGCTGTGTGTGAGACAGGTGTTTGTGTGTGGGGGGGGGGGGGGGGGGGGNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTGTTGATCTAACTTTCATGCTGCATGACACTGATTATGATCATGATCTCTTACTAAATCCACCAAATAGTTTAGGTTAGCCTGGGATCAAAGCTATGTAAATCGACTTTTATCATCAATAGTTGTGGTTTTTTGGGAACTCGAATTTTTTATACATGTCTATAGAATTTTATGGGCTGAGATGAAGTTTCTATTTATCATCTTCTTAACTCCTAAGGAATGAGATTCACATTTCTTATATGACTCAACAAGTAACCAAGTTATGTTTATTTTTTCCCTTTCATGATACAATAACTACTAGCAAAATTTTCAGCGTGGCAGTTGCTCAAAGGTGTCAGTCTATAATGCCAATTCATAAAACCTGTTTCTCTGTTTGTATAAAGCAGTAGATAAAGTGATCACTTTCTAACCATTCGTTTCTTTAACCTTTCATTTCAAGGTCATGCTGCCTGTTTCCTTTTAAACTGTTCATGCCTCGAGTATGAATTCAATTTGGGTAGCAAGCTTACCATTGCATGCTCTTCTAAACAGGTGCTTGATGCTTTGAGGACGACAATGTTCATACTTGGACTGATGTCGGTTTTCATAGGCATTTCTTTGCTAGCTCCCGATGAGCCTAGAGGTATTTTAATTTAGGTTCACATTAATTTTGGGATTGCATTGCAAGTATGAGAGCTTGATTGGAACATAAATTTCAGACGGGGAATTCAAGGATAATTCATCACTGGTTTCTGGGAAGTCCACTAGCATCTCGGCAGAAGAAGAGAGGTACATCAACTATTTTGCATGTTTAGGTGACATGAGTGTTTTCCAAAAAATCTTACGGAGTTCAAGACCACTTTCAAAAGGTGTCCTTGTTAGTTTTTATGGAAGACGTGAAGTATTCGACAATGAAAAATCTATGATCGATAAAGTGCTTATACTTTTGCCTTTTTATTTGCACCTCTTAACCCATTTCTGTTGCATACGTTTCATTTACAGACTAATCATGCCATCCAAAGAAGCAGCAGCAGAGACTAGGGAGGAAAGATCGTTTTCACAAGGACTCTTGTTAAAGATTTCAGATGTAATAGCCAAGGCAAAGGTACTGGAGTCCATTTCAATTTTGTAATTATCCTTTAAGCCTTGTTCTTTTGGATTAGAGCCTTTTATAGTTAGTTTAGTTTCTAGTTGGCTCCTCTTTCCGGCCTGTTATTTTGTTAGCACTTCTGTGATCTTTCACTCATTTTTTTTCCCTAAAAAAAAAGGGGATTTTTCAAGCTAACTTGCTTGATGGTCAACATGTTTAATCTGTTTAACCACATGCCGTCTTATTCTTTTCACAGGCTGCTTTGGCACTTTCTCTGGGTTTTGGAGAAGTTTCACTGAATGCATCTGCAGTACTTGTGATGCCCATGGTGTCATCAAGGATGACTGGCTTTAGAGGAACTGCATTGGAGCGCCCGAAGTTGTTCTCCACGAGGAAGCCTGATTGGAGTAAGGTCCCGTTGGATGAGGATACAAAGGTGCTCGACACAAACATAGGTTTCTCCTAAAGCCTTTGAATAGGAGTAGCAGTAAAAGAAAAATTCGGCTGGAGTGTTAAATCAATGCCTTTTCTCACTCTCTATAGGTATAGTAGTTAGGATTCATTATACCCGTTATTAGCATGTTCTTACATAGAATTATAAAAGACAATATTGTATATTATGAAGTAACATTTTTTTCCTCATAAAGCAAGATAATATTTCCTCCCAGTTTTTAGAAGACTCAAACCTTACGATTATATGGGAAGAGCAGTCTTAGTGCTGAAATGATTCCTTTCCGAACTTCCTATAGCCATGCCCAACTTTTGGAGTTTAAGTTGTATCAATATTGAAAGATAATATAATTTAGGCTTAAA

mRNA sequence

CACTATTCTCTCCGTTCCCAAATTGACCGCTCAACTTCTCCTCTCATTCACTTTTTTCTTTAATTAATTCATTATTATTTCATCTTCTCCCTTCTCCCCTCTCTGCAATCTACTCCGCTTCCCCATTTCTCTCTCCTTCTCCGGCGTCCGACGCTTTTCTCCACATTTCGGATTCCCGTGGGAGGGAACAAGCAAAGCAACTCCATCCCAATTCCAAGCCCGCGATTTCCCAATGCCCCACTTCCCAGATTCCATCTTCATCTTCTCAACTGCGCCCCATCTGATTCTGCACTGACCCATCTTCCCCACAGCCGCAGGCTGGGATCCCAAGCTGTATGCAGATTCGCTCACACGTCTTTAATTTATGGGGGATTGGGTTATTGGAGCTTTCATCAACCTCTTTGGCAGCATTGCCATCAACTTTGGGACCAACCTTCTTAAATTAGGCCATAACGAGAGAGAGAAGCATTCTATGCTAGAAAATAATGGCCCTAATGGAAAGACACCAATTAAGCCCATTATATACTTCCAGACTTGGAGAGTTGGTATTACATTTTTCATTCTTGGAAATTGCCTTAATTTTATTTCCTTTGGATATGCTGCCCAGTCTCTTCTTGCGGCACTTGGATCTGTACAGTTTGTGTCCAATATTGCTTTTGCATACTTCGTCCTCCACAAGATGGTTACTGTTAAGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAACATTTTTCTAGTTGCTTTTGGCAACCACCAGTCCCCTGTGTACACTCCAGAACAGCTGGTGGAGAAATATGGAAATATAACTTTTCTTCTATACTGCGTAATTTTGATCTTGATTGTTGCTTTACATCACTCAATCTATAGAAGGGGAGAATTGTTACTTTCTGATTCTGGACAAGATCTTAGACCGTATTGGCATATGCTGCTACCATTTTCCTATGCTATAGTTTCAGGTGCAATAGGCTCCTGCTCAGTCTTGTTTGCAAAATCTCTATTGGCCATGTCTAGTGCTTACGAGCTGCATAGCTGGTTTACATACTCCTTGCTGCTTTTATTTCTCAGTACTGCTGGATTTTGGATGGCCAGGTTAAACGAAGGACTTTCCCTGTTTGATGCAATCCTTATTGTTCCCATGTTTCAGATTGCTTGGACTTTCTTCTCCATATGTACAGGTTTTATATACTTTAAAGAATTCCAGGTGCTTGATGCTTTGAGGACGACAATGTTCATACTTGGACTGATGTCGGTTTTCATAGGCATTTCTTTGCTAGCTCCCGATGAGCCTAGAGACGGGGAATTCAAGGATAATTCATCACTGGTTTCTGGGAAGTCCACTAGCATCTCGGCAGAAGAAGAGAGACTAATCATGCCATCCAAAGAAGCAGCAGCAGAGACTAGGGAGGAAAGATCGTTTTCACAAGGACTCTTGTTAAAGATTTCAGATGTAATAGCCAAGGCAAAGGCTGCTTTGGCACTTTCTCTGGGTTTTGGAGAAGTTTCACTGAATGCATCTGCAGTACTTGTGATGCCCATGGTGTCATCAAGGATGACTGGCTTTAGAGGAACTGCATTGGAGCGCCCGAAGTTGTTCTCCACGAGGAAGCCTGATTGGAGTAAGGTCCCGTTGGATGAGGATACAAAGGTGCTCGACACAAACATAGGTTTCTCCTAAAGCCTTTGAATAGGAGTAGCAGTAAAAGAAAAATTCGGCTGGAGTGTTAAATCAATGCCTTTTCTCACTCTCTATAGGTATAGTAGTTAGGATTCATTATACCCGTTATTAGCATGTTCTTACATAGAATTATAAAAGACAATATTGTATATTATGAAGTAACATTTTTTTCCTCATAAAGCAAGATAATATTTCCTCCCAGTTTTTAGAAGACTCAAACCTTACGATTATATGGGAAGAGCAGTCTTAGTGCTGAAATGATTCCTTTCCGAACTTCCTATAGCCATGCCCAACTTTTGGAGTTTAAGTTGTATCAATATTGAAAGATAATATAATTTAGGCTTAAA

Coding sequence (CDS)

ATGGGGGATTGGGTTATTGGAGCTTTCATCAACCTCTTTGGCAGCATTGCCATCAACTTTGGGACCAACCTTCTTAAATTAGGCCATAACGAGAGAGAGAAGCATTCTATGCTAGAAAATAATGGCCCTAATGGAAAGACACCAATTAAGCCCATTATATACTTCCAGACTTGGAGAGTTGGTATTACATTTTTCATTCTTGGAAATTGCCTTAATTTTATTTCCTTTGGATATGCTGCCCAGTCTCTTCTTGCGGCACTTGGATCTGTACAGTTTGTGTCCAATATTGCTTTTGCATACTTCGTCCTCCACAAGATGGTTACTGTTAAGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAACATTTTTCTAGTTGCTTTTGGCAACCACCAGTCCCCTGTGTACACTCCAGAACAGCTGGTGGAGAAATATGGAAATATAACTTTTCTTCTATACTGCGTAATTTTGATCTTGATTGTTGCTTTACATCACTCAATCTATAGAAGGGGAGAATTGTTACTTTCTGATTCTGGACAAGATCTTAGACCGTATTGGCATATGCTGCTACCATTTTCCTATGCTATAGTTTCAGGTGCAATAGGCTCCTGCTCAGTCTTGTTTGCAAAATCTCTATTGGCCATGTCTAGTGCTTACGAGCTGCATAGCTGGTTTACATACTCCTTGCTGCTTTTATTTCTCAGTACTGCTGGATTTTGGATGGCCAGGTTAAACGAAGGACTTTCCCTGTTTGATGCAATCCTTATTGTTCCCATGTTTCAGATTGCTTGGACTTTCTTCTCCATATGTACAGGTTTTATATACTTTAAAGAATTCCAGGTGCTTGATGCTTTGAGGACGACAATGTTCATACTTGGACTGATGTCGGTTTTCATAGGCATTTCTTTGCTAGCTCCCGATGAGCCTAGAGACGGGGAATTCAAGGATAATTCATCACTGGTTTCTGGGAAGTCCACTAGCATCTCGGCAGAAGAAGAGAGACTAATCATGCCATCCAAAGAAGCAGCAGCAGAGACTAGGGAGGAAAGATCGTTTTCACAAGGACTCTTGTTAAAGATTTCAGATGTAATAGCCAAGGCAAAGGCTGCTTTGGCACTTTCTCTGGGTTTTGGAGAAGTTTCACTGAATGCATCTGCAGTACTTGTGATGCCCATGGTGTCATCAAGGATGACTGGCTTTAGAGGAACTGCATTGGAGCGCCCGAAGTTGTTCTCCACGAGGAAGCCTGATTGGAGTAAGGTCCCGTTGGATGAGGATACAAAGGTGCTCGACACAAACATAGGTTTCTCCTAA

Protein sequence

MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVLGNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLLAMSSAYELHSWFTYSLLLLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLMSVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQGLLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRKPDWSKVPLDEDTKVLDTNIGFS
Homology
BLAST of MC08g1160 vs. ExPASy Swiss-Prot
Match: Q8RWF4 (Probable magnesium transporter NIPA8 OS=Arabidopsis thaliana OX=3702 GN=At3g26670 PE=2 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.1e-168
Identity = 314/441 (71.20%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGAFIN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ QTWRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQDG---GGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN+FLVAFGNHQSPV+TPEQL EKY N+TFL+YC ILILIVA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYA+VSGAIGSCSVLFAKSL     LAMSS+Y+LHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWM RLNEGLSL+DAILIVPMFQIAWTFFSICTG IYF+EFQV DALRTTMFILG+M
Sbjct: 241 TAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDN-SSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQ 360
            VFIGISLLAPD+ R  E KDN SSL S  S+S+  EE+RLI P       +++ R   Q
Sbjct: 301 CVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLI-PQSSEDGHSKDTRVVVQ 360

Query: 361 GLLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTR 420
           G+ +K +D+IAK K A   +LGFGE S+NASA+LVMPMVSS++TGFRG  LER K+ S R
Sbjct: 361 GMYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMR 420

Query: 421 KPDWSKVPLDED-TKVLDTNI 435
              WSK+ ++E+ T++L+  I
Sbjct: 421 GSGWSKLAMEEEGTRMLEKTI 437

BLAST of MC08g1160 vs. ExPASy Swiss-Prot
Match: Q9LIR9 (Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana OX=3702 GN=At3g23870 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.3e-08
Identity = 54/254 (21.26%), Postives = 118/254 (46.46%), Query Frame = 0

Query: 58  WRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAF 117
           W  G+   I+G   NF ++ +A   L+  LG++  + +   A+F+L + + +  ++    
Sbjct: 56  WWAGMITMIVGEVANFAAYAFAPAILVTPLGALSIIFSAVLAHFILKEKLHMFGILGCIL 115

Query: 118 IVLGNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIY--RRGELLL 177
            V+G+  +V    H+  + + +Q+ +      FL+Y  +++++VA+    Y  R G+   
Sbjct: 116 CVVGSTTIVLHAPHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKT-- 175

Query: 178 SDSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLL 237
                      HM++   Y  +   +GS +V+  K++     L  S   +   + T+  +
Sbjct: 176 -----------HMIV---YVGICSLMGSLTVMSVKAVAIAIKLTFSGTNQFKYFNTWIFI 235

Query: 238 LLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMF 297
           L+  +     +  LN+ L  F+  +I P++ + +T F+I    I FK++     L+    
Sbjct: 236 LVVATCCILQINYLNKALDTFNTAVISPVYYVMFTTFTIIASMIMFKDWASQSGLKIATE 293

Query: 298 ILGLMSVFIGISLL 305
           + G +++  G  LL
Sbjct: 296 LCGFVTILSGTFLL 293

BLAST of MC08g1160 vs. ExPASy Swiss-Prot
Match: Q8BGN5 (NIPA-like protein 3 OS=Mus musculus OX=10090 GN=Nipal3 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.6e-07
Identity = 74/329 (22.49%), Postives = 136/329 (41.34%), Query Frame = 0

Query: 5   VIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRVGITF 64
           +IGA + +FG + ++   NL K  H               G    +     +TW +G+  
Sbjct: 38  LIGALLAIFGHLVVSIALNLQKYCHIRLA-----------GSKDPRAYFKTKTWWLGLLL 97

Query: 65  FILGNCLNFISFGYAAQSLLAALGSVQFV-SNIAFAYFVLHKMVT-------VKVMVATA 124
            +LG    F S+ +A  SL+  L +V  + S I    F+  K          V   V   
Sbjct: 98  LLLGELGVFASYAFAPLSLIVPLSAVSVIASAIIGIIFIKEKWKPKDFVRRYVLSFVGCG 157

Query: 125 FIVLGNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLS 184
             ++G   LV F  +     T E +     +  FLLY ++ I++  L    Y+       
Sbjct: 158 LAIVGTYLLVTFAPNSHEKMTGENIARHLVSWPFLLYMLVAIVLFCLLLYFYKE------ 217

Query: 185 DSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKS-----LLAMSSAYELHSWFTYSLLL 244
              ++      +LL      +   +GS +V+  K+     +L++    +L     Y + +
Sbjct: 218 ---RNANSIVVILL------LVALLGSMTVVTVKAVSGMLVLSIQGNLQLDYPIFYVMFV 277

Query: 245 LFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFI 304
             ++TA +    L+E   ++D+ LI  +  I  T  +I  G I++ +F   +AL   MF 
Sbjct: 278 CMVATAIYQATFLSEASQIYDSSLIASVGYILSTTAAITAGAIFYLDFLGEEALHICMFA 337

Query: 305 LGLMSVFIGISLLAPDEPRDGEFKDNSSL 321
           LG +  F+G+ L+  +  +   F+   S+
Sbjct: 338 LGCLIAFLGVFLITRNRKKAIPFEPYISM 340

BLAST of MC08g1160 vs. ExPASy Swiss-Prot
Match: Q6P499 (NIPA-like protein 3 OS=Homo sapiens OX=9606 GN=NIPAL3 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.1e-07
Identity = 86/379 (22.69%), Postives = 154/379 (40.63%), Query Frame = 0

Query: 5   VIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRVGITF 64
           +IGA + +FG + ++   NL K  H               G    +     +TW +G+  
Sbjct: 34  LIGALLAIFGHLVVSIALNLQKYCHIRLA-----------GSKDPRAYFKTKTWWLGLFL 93

Query: 65  FILGNCLNFISFGYAAQSLLAALGSVQFV-SNIAFAYFVLHKMVT-------VKVMVATA 124
            +LG    F S+ +A  SL+  L +V  + S I    F+  K          V   V   
Sbjct: 94  MLLGELGVFASYAFAPLSLIVPLSAVSVIASAIIGIIFIKEKWKPKDFLRRYVLSFVGCG 153

Query: 125 FIVLGNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLS 184
             V+G   LV F  +     T E +     +  FLLY ++ I++  L    Y+       
Sbjct: 154 LAVVGTYLLVTFAPNSHEKMTGENVTRHLVSWPFLLYMLVEIILFCLLLYFYKE------ 213

Query: 185 DSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKS-----LLAMSSAYELHSWFTYSLLL 244
              ++      +LL      +   +GS +V+  K+     +L++    +L     Y + +
Sbjct: 214 ---KNANNIVVILL------LVALLGSMTVVTVKAVAGMLVLSIQGNLQLDYPIFYVMFV 273

Query: 245 LFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFI 304
             ++TA +  A L++   ++D+ LI  +  I  T  +I  G I++ +F   D L   MF 
Sbjct: 274 CMVATAVYQAAFLSQASQMYDSSLIASVGYILSTTIAITAGAIFYLDFIGEDVLHICMFA 333

Query: 305 LGLMSVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERS 364
           LG +  F+G+ L+  +  +   F+   S+ +        ++   + P  +A        S
Sbjct: 334 LGCLIAFLGVFLITRNRKKPIPFEPYISMDAMPGMQNMHDKGMTVQPELKA--------S 378

Query: 365 FSQGLLL---KISDVIAKA 368
           FS G L     IS++ A A
Sbjct: 394 FSYGALENNDNISEIYAPA 378

BLAST of MC08g1160 vs. ExPASy Swiss-Prot
Match: Q5RD30 (NIPA-like protein 3 OS=Pongo abelii OX=9601 GN=NIPAL3 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.6e-07
Identity = 85/379 (22.43%), Postives = 154/379 (40.63%), Query Frame = 0

Query: 5   VIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRVGITF 64
           +IGA + +FG + ++   NL K  H               G    +     +TW +G+  
Sbjct: 34  LIGALLAIFGHLVVSIALNLQKYCHIRLA-----------GSKDPRAYFKTKTWWLGLFL 93

Query: 65  FILGNCLNFISFGYAAQSLLAALGSVQFV-SNIAFAYFVLHKMVT-------VKVMVATA 124
            +LG    F S+ +A  SL+  L +V  + S I    F+  K          +   V   
Sbjct: 94  MLLGELGVFASYAFAPLSLIVPLSAVSVIASAIIGIIFIKEKWKPKDFLRRYILSFVGCG 153

Query: 125 FIVLGNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLS 184
             V+G   LV F  +     T E +     +  FLLY ++ I++  L    Y+       
Sbjct: 154 LAVVGTYLLVTFAPNSREKMTGENVTRHLVSWPFLLYMLVEIILFCLLLYFYKE------ 213

Query: 185 DSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKS-----LLAMSSAYELHSWFTYSLLL 244
              ++      +LL      +   +GS +V+  K+     +L++    +L     Y + +
Sbjct: 214 ---KNANNIVVILL------LVALLGSMTVVTVKAVAGMLVLSIQGNLQLDYPIFYVMFV 273

Query: 245 LFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFI 304
             ++TA +  A L++   ++D+ LI  +  I  T  +I  G I++ +F   D L   MF 
Sbjct: 274 CMVATAVYQAAFLSQASQMYDSSLIASVGYILSTTIAITAGAIFYLDFIGEDVLHICMFA 333

Query: 305 LGLMSVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERS 364
           LG +  F+G+ L+  +  +   F+   S+ +        ++   + P  +A        S
Sbjct: 334 LGCLIAFLGVFLITRNRKKAIPFEPYISMDAMPGMQNMHDKGMTVQPELKA--------S 378

Query: 365 FSQGLLL---KISDVIAKA 368
           FS G L     IS++ A A
Sbjct: 394 FSYGALENNDNISEIYAPA 378

BLAST of MC08g1160 vs. NCBI nr
Match: XP_022148208.1 (probable magnesium transporter NIPA8 isoform X1 [Momordica charantia])

HSP 1 Score: 829 bits (2142), Expect = 2.31e-302
Identity = 437/442 (98.87%), Postives = 437/442 (98.87%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV
Sbjct: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ
Sbjct: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSSAYELHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSAYELHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
           LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK
Sbjct: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420

Query: 421 PDWSKVPLDEDTKVLDTNIGFS 437
           PDWSKVPLDEDTKVLDTNIGFS
Sbjct: 421 PDWSKVPLDEDTKVLDTNIGFS 442

BLAST of MC08g1160 vs. NCBI nr
Match: XP_022148209.1 (probable magnesium transporter NIPA8 isoform X2 [Momordica charantia])

HSP 1 Score: 778 bits (2009), Expect = 1.93e-282
Identity = 416/442 (94.12%), Postives = 416/442 (94.12%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV
Sbjct: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ
Sbjct: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSSAYELHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSAYELHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFW                     IAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM
Sbjct: 241 TAGFW---------------------IAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
           LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK
Sbjct: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420

Query: 421 PDWSKVPLDEDTKVLDTNIGFS 437
           PDWSKVPLDEDTKVLDTNIGFS
Sbjct: 421 PDWSKVPLDEDTKVLDTNIGFS 421

BLAST of MC08g1160 vs. NCBI nr
Match: XP_038877527.1 (probable magnesium transporter NIPA8 isoform X1 [Benincasa hispida] >XP_038877534.1 probable magnesium transporter NIPA8 isoform X1 [Benincasa hispida])

HSP 1 Score: 761 bits (1965), Expect = 2.23e-275
Identity = 400/442 (90.50%), Postives = 417/442 (94.34%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGA INLFGSIAINFGTNLLKLGHNEREKHSMLENNG +GKTP+KPIIYFQTWRV
Sbjct: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSSGKTPLKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN+FLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIL+VALHHSIYRRGELLLS SGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSS Y+LHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILG+M
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRD +FKDNSSLVSG STS ++EEERLI+ SKE   ET++ RSFSQG
Sbjct: 301 SVFIGISLLAPDEPRDCDFKDNSSLVSGTSTSFTSEEERLIVSSKEL--ETKDARSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
            LLKI+DV+AKAKAALALSLGFGE SLNASA+LVMPMVSSRMTGFRGT  ER K FS RK
Sbjct: 361 FLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTGFRGTGFERSKFFSMRK 420

Query: 421 PDWSKVPLDEDTKVLDTNIGFS 437
           PDWSK+ LDEDTKVLDTNIGFS
Sbjct: 421 PDWSKISLDEDTKVLDTNIGFS 440

BLAST of MC08g1160 vs. NCBI nr
Match: XP_004145621.2 (probable magnesium transporter NIPA8 [Cucumis sativus] >XP_031738794.1 probable magnesium transporter NIPA8 [Cucumis sativus] >KAE8650557.1 hypothetical protein Csa_010669 [Cucumis sativus])

HSP 1 Score: 755 bits (1949), Expect = 6.34e-273
Identity = 398/443 (89.84%), Postives = 418/443 (94.36%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGA INLFGS+AINFGTNLLKLGHNEREKHSMLENNG  GKTP+KPIIYFQTWRV
Sbjct: 1   MGEWVIGAVINLFGSLAINFGTNLLKLGHNEREKHSMLENNGSIGKTPMKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN+FLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIL+VALHHSIYRRGELLLS SGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSS Y+LHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILG+M
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNSSLVSG STS ++EEERLI+ SKE   ETR+ +SFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFTSEEERLIVSSKEL--ETRDAKSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
           +LLKI+DV+AKAKAALALSLGFGE SLNASA+LVMPMVSSRMTGFRGT  ER K FS RK
Sbjct: 361 VLLKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTGFRGTGFERSKFFSMRK 420

Query: 421 PDWSKVPLDE-DTKVLDTNIGFS 437
           PDWS++ LDE DTKVLDT+IGFS
Sbjct: 421 PDWSRISLDEEDTKVLDTDIGFS 441

BLAST of MC08g1160 vs. NCBI nr
Match: XP_008449977.1 (PREDICTED: probable magnesium transporter NIPA8 [Cucumis melo] >XP_008449978.1 PREDICTED: probable magnesium transporter NIPA8 [Cucumis melo])

HSP 1 Score: 753 bits (1945), Expect = 2.58e-272
Identity = 398/443 (89.84%), Postives = 417/443 (94.13%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGA INLFGS+AINFGTNLLKLGHNEREKHSMLENNG  GKTP+KPIIYFQTWRV
Sbjct: 1   MGEWVIGAVINLFGSLAINFGTNLLKLGHNEREKHSMLENNGSIGKTPMKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN+FLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILI++VALHHSIYRRGELLLS SGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIVVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLR YWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSS Y+LHSWFTYSLLLLFLS
Sbjct: 181 DLRSYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILG+M
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNSSLVSG STS ++EEERLI+ SKE+  ETR+ +SFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFTSEEERLIVSSKES--ETRDAKSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
            LLKI+DV+AKAKAALALSLGFGE SLNASAVLVMPMVSSRMTGFRGT  ER K FS RK
Sbjct: 361 FLLKITDVVAKAKAALALSLGFGEDSLNASAVLVMPMVSSRMTGFRGTGFERSKFFSMRK 420

Query: 421 PDWSKVPLDE-DTKVLDTNIGFS 437
           PDWS++ LDE DTKVLDTNIGFS
Sbjct: 421 PDWSRISLDEEDTKVLDTNIGFS 441

BLAST of MC08g1160 vs. ExPASy TrEMBL
Match: A0A6J1D3G2 (Probable magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111016932 PE=3 SV=1)

HSP 1 Score: 829 bits (2142), Expect = 1.12e-302
Identity = 437/442 (98.87%), Postives = 437/442 (98.87%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV
Sbjct: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ
Sbjct: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSSAYELHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSAYELHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
           LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK
Sbjct: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420

Query: 421 PDWSKVPLDEDTKVLDTNIGFS 437
           PDWSKVPLDEDTKVLDTNIGFS
Sbjct: 421 PDWSKVPLDEDTKVLDTNIGFS 442

BLAST of MC08g1160 vs. ExPASy TrEMBL
Match: A0A6J1D3B3 (Probable magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111016932 PE=3 SV=1)

HSP 1 Score: 778 bits (2009), Expect = 9.36e-283
Identity = 416/442 (94.12%), Postives = 416/442 (94.12%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV
Sbjct: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ
Sbjct: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSSAYELHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSAYELHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFW                     IAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM
Sbjct: 241 TAGFW---------------------IAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
           LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK
Sbjct: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420

Query: 421 PDWSKVPLDEDTKVLDTNIGFS 437
           PDWSKVPLDEDTKVLDTNIGFS
Sbjct: 421 PDWSKVPLDEDTKVLDTNIGFS 421

BLAST of MC08g1160 vs. ExPASy TrEMBL
Match: A0A1S3BN84 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103491699 PE=3 SV=1)

HSP 1 Score: 753 bits (1945), Expect = 1.25e-272
Identity = 398/443 (89.84%), Postives = 417/443 (94.13%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGA INLFGS+AINFGTNLLKLGHNEREKHSMLENNG  GKTP+KPIIYFQTWRV
Sbjct: 1   MGEWVIGAVINLFGSLAINFGTNLLKLGHNEREKHSMLENNGSIGKTPMKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN+FLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILI++VALHHSIYRRGELLLS SGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIVVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLR YWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSS Y+LHSWFTYSLLLLFLS
Sbjct: 181 DLRSYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILG+M
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNSSLVSG STS ++EEERLI+ SKE+  ETR+ +SFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFTSEEERLIVSSKES--ETRDAKSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
            LLKI+DV+AKAKAALALSLGFGE SLNASAVLVMPMVSSRMTGFRGT  ER K FS RK
Sbjct: 361 FLLKITDVVAKAKAALALSLGFGEDSLNASAVLVMPMVSSRMTGFRGTGFERSKFFSMRK 420

Query: 421 PDWSKVPLDE-DTKVLDTNIGFS 437
           PDWS++ LDE DTKVLDTNIGFS
Sbjct: 421 PDWSRISLDEEDTKVLDTNIGFS 441

BLAST of MC08g1160 vs. ExPASy TrEMBL
Match: A0A6J1F408 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111442184 PE=3 SV=1)

HSP 1 Score: 744 bits (1922), Expect = 3.83e-269
Identity = 392/442 (88.69%), Postives = 412/442 (93.21%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGA INLFGSIAINFGTNLLKLGHNEREKHSMLENNG NGK+P+KPIIYFQTWR+
Sbjct: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKSPMKPIIYFQTWRI 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           G+TFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLH+MVTVKVMVATAFIVL
Sbjct: 61  GLTFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHEMVTVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN FLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILI +VALHHSIYRRGELLLS SGQ
Sbjct: 121 GNSFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIFVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL     LAMSS Y+LHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILG+M
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNS L+S  STS S+EEERLI+ S+E   ET++ RSFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSPLLSETSTSFSSEEERLIVSSQEL--ETKDARSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
            LLKI+DV+AK KAALALSLGFGE SLNASAVLVMPMVSSRMTGFRGT LER K FS RK
Sbjct: 361 FLLKITDVVAKGKAALALSLGFGEDSLNASAVLVMPMVSSRMTGFRGTGLERSKPFSMRK 420

Query: 421 PDWSKVPLDEDTKVLDTNIGFS 437
            DWSK+ LDED KVLDT+IGFS
Sbjct: 421 SDWSKISLDEDAKVLDTSIGFS 440

BLAST of MC08g1160 vs. ExPASy TrEMBL
Match: A0A6J1J669 (Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111482128 PE=3 SV=1)

HSP 1 Score: 739 bits (1907), Expect = 7.37e-267
Identity = 390/442 (88.24%), Postives = 411/442 (92.99%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGA INLFGSIAINFGTNLLKLGHNEREKHSMLENNG NGKTP+KPIIYFQTWR+
Sbjct: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRI 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           G+TFFI+GNCLNFISFGYAAQSLLAALGSVQFV+NIAFAYFVLH+MV VKVMVATAFIVL
Sbjct: 61  GLTFFIIGNCLNFISFGYAAQSLLAALGSVQFVANIAFAYFVLHEMVAVKVMVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN FLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIL+VALHHSIYRRGELLLS S Q
Sbjct: 121 GNSFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSRQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           DLRPYW MLLPFSYAIVSGAIGSCSVLFAKSL     LAMSS Y+LHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWRMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQG 360
           SVFIGISLLAPDEPRDGEFKDNS+L+S  STS S+EEERLI+ S+E   ET++ RSFSQG
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSTLLSETSTSFSSEEERLIVSSQEL--ETKDARSFSQG 360

Query: 361 LLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTRK 420
            LLKI+DV+AK K+ALALSLGFGE SLNASAVLVMPMVSSRMTGFRGT LER KLFS RK
Sbjct: 361 FLLKITDVVAKGKSALALSLGFGEDSLNASAVLVMPMVSSRMTGFRGTGLERSKLFSMRK 420

Query: 421 PDWSKVPLDEDTKVLDTNIGFS 437
             WSK+ LDED KVLDT+IGFS
Sbjct: 421 SGWSKISLDEDAKVLDTSIGFS 440

BLAST of MC08g1160 vs. TAIR 10
Match: AT3G26670.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 594.3 bits (1531), Expect = 7.7e-170
Identity = 314/441 (71.20%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGAFIN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ QTWRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQDG---GGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN+FLVAFGNHQSPV+TPEQL EKY N+TFL+YC ILILIVA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYA+VSGAIGSCSVLFAKSL     LAMSS+Y+LHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWM RLNEGLSL+DAILIVPMFQIAWTFFSICTG IYF+EFQV DALRTTMFILG+M
Sbjct: 241 TAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDN-SSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQ 360
            VFIGISLLAPD+ R  E KDN SSL S  S+S+  EE+RLI P       +++ R   Q
Sbjct: 301 CVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLI-PQSSEDGHSKDTRVVVQ 360

Query: 361 GLLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTR 420
           G+ +K +D+IAK K A   +LGFGE S+NASA+LVMPMVSS++TGFRG  LER K+ S R
Sbjct: 361 GMYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMR 420

Query: 421 KPDWSKVPLDED-TKVLDTNI 435
              WSK+ ++E+ T++L+  I
Sbjct: 421 GSGWSKLAMEEEGTRMLEKTI 437

BLAST of MC08g1160 vs. TAIR 10
Match: AT3G26670.2 (Protein of unknown function (DUF803) )

HSP 1 Score: 594.3 bits (1531), Expect = 7.7e-170
Identity = 314/441 (71.20%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGAFIN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ QTWRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQDG---GGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN+FLVAFGNHQSPV+TPEQL EKY N+TFL+YC ILILIVA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYA+VSGAIGSCSVLFAKSL     LAMSS+Y+LHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWM RLNEGLSL+DAILIVPMFQIAWTFFSICTG IYF+EFQV DALRTTMFILG+M
Sbjct: 241 TAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDN-SSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQ 360
            VFIGISLLAPD+ R  E KDN SSL S  S+S+  EE+RLI P       +++ R   Q
Sbjct: 301 CVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLI-PQSSEDGHSKDTRVVVQ 360

Query: 361 GLLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTR 420
           G+ +K +D+IAK K A   +LGFGE S+NASA+LVMPMVSS++TGFRG  LER K+ S R
Sbjct: 361 GMYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMR 420

Query: 421 KPDWSKVPLDED-TKVLDTNI 435
              WSK+ ++E+ T++L+  I
Sbjct: 421 GSGWSKLAMEEEGTRMLEKTI 437

BLAST of MC08g1160 vs. TAIR 10
Match: AT3G26670.3 (Protein of unknown function (DUF803) )

HSP 1 Score: 594.3 bits (1531), Expect = 7.7e-170
Identity = 314/441 (71.20%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MGDWVIGAFINLFGSIAINFGTNLLKLGHNEREKHSMLENNGPNGKTPIKPIIYFQTWRV 60
           MG+WVIGAFIN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ QTWRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQDG---GGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIYRRGELLLSDSGQ 180
           GN+FLVAFGNHQSPV+TPEQL EKY N+TFL+YC ILILIVA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYA+VSGAIGSCSVLFAKSL     LAMSS+Y+LHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300
           TAGFWM RLNEGLSL+DAILIVPMFQIAWTFFSICTG IYF+EFQV DALRTTMFILG+M
Sbjct: 241 TAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDN-SSLVSGKSTSISAEEERLIMPSKEAAAETREERSFSQ 360
            VFIGISLLAPD+ R  E KDN SSL S  S+S+  EE+RLI P       +++ R   Q
Sbjct: 301 CVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLI-PQSSEDGHSKDTRVVVQ 360

Query: 361 GLLLKISDVIAKAKAALALSLGFGEVSLNASAVLVMPMVSSRMTGFRGTALERPKLFSTR 420
           G+ +K +D+IAK K A   +LGFGE S+NASA+LVMPMVSS++TGFRG  LER K+ S R
Sbjct: 361 GMYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMR 420

Query: 421 KPDWSKVPLDED-TKVLDTNI 435
              WSK+ ++E+ T++L+  I
Sbjct: 421 GSGWSKLAMEEEGTRMLEKTI 437

BLAST of MC08g1160 vs. TAIR 10
Match: AT3G23870.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 61.2 bits (147), Expect = 2.3e-09
Identity = 54/254 (21.26%), Postives = 118/254 (46.46%), Query Frame = 0

Query: 58  WRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAF 117
           W  G+   I+G   NF ++ +A   L+  LG++  + +   A+F+L + + +  ++    
Sbjct: 56  WWAGMITMIVGEVANFAAYAFAPAILVTPLGALSIIFSAVLAHFILKEKLHMFGILGCIL 115

Query: 118 IVLGNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILIVALHHSIY--RRGELLL 177
            V+G+  +V    H+  + + +Q+ +      FL+Y  +++++VA+    Y  R G+   
Sbjct: 116 CVVGSTTIVLHAPHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKT-- 175

Query: 178 SDSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLL 237
                      HM++   Y  +   +GS +V+  K++     L  S   +   + T+  +
Sbjct: 176 -----------HMIV---YVGICSLMGSLTVMSVKAVAIAIKLTFSGTNQFKYFNTWIFI 235

Query: 238 LLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMF 297
           L+  +     +  LN+ L  F+  +I P++ + +T F+I    I FK++     L+    
Sbjct: 236 LVVATCCILQINYLNKALDTFNTAVISPVYYVMFTTFTIIASMIMFKDWASQSGLKIATE 293

Query: 298 ILGLMSVFIGISLL 305
           + G +++  G  LL
Sbjct: 296 LCGFVTILSGTFLL 293

BLAST of MC08g1160 vs. TAIR 10
Match: AT1G34470.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 55.5 bits (132), Expect = 1.3e-07
Identity = 57/254 (22.44%), Postives = 113/254 (44.49%), Query Frame = 0

Query: 58  WRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAF 117
           W VG+   I+G   NF ++ +A   L+  LG++  + + A A+ +LH+ +    ++    
Sbjct: 67  WWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVL 126

Query: 118 IVLGNIFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILI--LIVALHHSIYRRGELLL 177
            V+G+I +V     +  + +  Q+        FLLY   ++   I+ +   + + G+   
Sbjct: 127 CVVGSITIVLHAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQS-- 186

Query: 178 SDSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSL-----LAMSSAYELHSWFTYSLL 237
                      H+++   Y  V   +GS SV+  K+L     L  S   +L    T+   
Sbjct: 187 -----------HVMV---YIGVCSLVGSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFT 246

Query: 238 LLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMF 297
           L+ L+     M  LN+ L  F+  ++ P++ + +T  +I    I FK++   D  +    
Sbjct: 247 LIVLTCVITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTE 304

Query: 298 ILGLMSVFIGISLL 305
           + G +++  G  LL
Sbjct: 307 LCGFVTILSGTFLL 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWF41.1e-16871.20Probable magnesium transporter NIPA8 OS=Arabidopsis thaliana OX=3702 GN=At3g2667... [more]
Q9LIR93.3e-0821.26Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana OX=3702 GN=At3g2387... [more]
Q8BGN51.6e-0722.49NIPA-like protein 3 OS=Mus musculus OX=10090 GN=Nipal3 PE=2 SV=1[more]
Q6P4992.1e-0722.69NIPA-like protein 3 OS=Homo sapiens OX=9606 GN=NIPAL3 PE=1 SV=1[more]
Q5RD303.6e-0722.43NIPA-like protein 3 OS=Pongo abelii OX=9601 GN=NIPAL3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022148208.12.31e-30298.87probable magnesium transporter NIPA8 isoform X1 [Momordica charantia][more]
XP_022148209.11.93e-28294.12probable magnesium transporter NIPA8 isoform X2 [Momordica charantia][more]
XP_038877527.12.23e-27590.50probable magnesium transporter NIPA8 isoform X1 [Benincasa hispida] >XP_03887753... [more]
XP_004145621.26.34e-27389.84probable magnesium transporter NIPA8 [Cucumis sativus] >XP_031738794.1 probable ... [more]
XP_008449977.12.58e-27289.84PREDICTED: probable magnesium transporter NIPA8 [Cucumis melo] >XP_008449978.1 P... [more]
Match NameE-valueIdentityDescription
A0A6J1D3G21.12e-30298.87Probable magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111016932 PE... [more]
A0A6J1D3B39.36e-28394.12Probable magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111016932 PE... [more]
A0A1S3BN841.25e-27289.84Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103491699 PE=3 SV=1[more]
A0A6J1F4083.83e-26988.69Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111442184 PE=... [more]
A0A6J1J6697.37e-26788.24Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111482128 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G26670.17.7e-17071.20Protein of unknown function (DUF803) [more]
AT3G26670.27.7e-17071.20Protein of unknown function (DUF803) [more]
AT3G26670.37.7e-17071.20Protein of unknown function (DUF803) [more]
AT3G23870.12.3e-0921.26Protein of unknown function (DUF803) [more]
AT1G34470.11.3e-0722.44Protein of unknown function (DUF803) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 4..305
e-value: 1.3E-16
score: 60.6
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 1..426
NoneNo IPR availablePANTHERPTHR12570:SF75MAGNESIUM TRANSPORTER-RELATEDcoord: 1..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1160.1MC08g1160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0015693 magnesium ion transport
cellular_component GO:0005769 early endosome
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity