MC08g1128 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1128
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description(S)-2-hydroxy-acid oxidase
LocationMC08: 9452965 .. 9458600 (+)
RNA-Seq ExpressionMC08g1128
SyntenyMC08g1128
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCGACTTATGTTGTGATGGGGAATACTTTTCTTCAAAAAAATTGAACGGTCTACTGTGTTTTACTCTAGAAATTTGCATCCTTTGATAAATTCTTCTCGTTTTTCCCCATAACAATGTAAAATTTTTACTATCTATTAATAGTTAAGAAAATACAATAGCATTTTTAAGTTCGACGCTTTCAATCCCCATCGGAGTAAATAAATTGCATATAAGTTCATCTAAAGATCGACTTCTAGTAGGTCCAATAATTAGCAATATGCCTTTCAAAAAAATAAAAAATAAATAATTGGCAACATGTCTTCAACTTTTAGTTGAACTTATGTTTTATTCAAGTTGGTGCTCTCGTAGAGCTAACCAGCTTGACCCATTCTGTATTGAAGACTGCACGGGTCAAAATAAGAGCGTCAAGTTAATAATTGATGTCAGCTCTGCCTTATCCATTGGCTCACTGTTTTTCCTCAATTTCAACATCCTCCTGGTGATATCTGTAAGCTTTATTTTGCTGATCTACGGCTTTCTCAAGTGTCTTTTTAAGAAGATTATTTATATTCCGTTCCATTATGAATCTCAAGTTTAGTGGGTCATCTTGATTCTTTCTGGTCGTGGTGTCTCTGAAATGATTCCTGCTTCAGTTTGTACGTATCAAGATATCAAGAGGGTCGCATCATAAAAGAATTCTAAGTTAGTATCTTGTGACGCTGTGTGCGTGTGTTGAGTTTGTGTCATGAATTTTGGCCAACTTGTATGGTGCTGTAACAGTTCCAGCGGGGAAACTGGAAACTCTCATCTCCCCATCCTCAAGAAGTGAACTAAGTAAGATATTTGCCATAGGTTTTCTGGCCAAAATAAATGAGAGAGAGTATAAATCAATGGGTGTACTAATTGTACTCTTTGTCCTCCCTAGAAGTGGGATTACTTTCCCCGTTTTGAGAAGAGAAATTACACATCCAAGGAATTTCTATTGGAAGAAGATGTACCAAAACTTGGCACAATCACTGTAACTGTGCATCCTGGTTTAGAACTTCAAGTAATGTTTTTATTTGATTGCCAGGTGTTGTACAGATCTTATTTTTGCCTCATATGAGTTATATCTATTTGGATGCATCATCCTGCTATACACACATGCTTAGTTCATGGTACCAAAAGCTCTAAAAAAATAATGTTCTAGTCTCTGTTTTCTCAGGATAAAATGTCATCTGAACCAGTGAATGTGAATGACTATAAAGAATTGGCTAGGCAAGCACTTCCAAAAATGTATTATGATTACTATGCGGGAGGAGCTGAGGACCAGCACACACTTAGAGAAAACATGCAAGCTTTCCATAGAATCATGTAATTTTGAAGCTAGCTTATGATTACTGTGAAAAGTGATGCTATATGTTATTCATGAAATTTTAATTGTTTTCAGTATCCGACCTCGAATTCTTGTCGATGTAAGCAAAATTGATATGTCAACAACCATATTGGGCTACCTCATATCTGAACCTATCATGGTTGCTCCAACTGGTGCACACAAATTGGCACACTGTGAAGGTTAAAATTTTTGGTGGATTGCTTAGTTGAGAAATGGATAAATGGTTATCATTCCACTTTTCCTGGTTGATATTTGTCTTCTTTCAGGAGAGCTAGCCACAGCAAGGGCAGCAGCAGCTGCAAAAACCATAATGGTACTAATGTGACTGCAAACTGGAAGCCATTTTACTCATTTGGTATTTGAACTAGTTGGTTTTGTGTGTAGGTTCTATCATATGCCTCATCCTACTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTTCGTTTTTTTCAATTATATGTAAATCCCACTCCTTCGAACTTTTCCTGTAGCAGAAATCAATTGTTTGTTTTTTTAATTGAACTTCTCATTCTGTAATCAGGTTTTCAGAAAGCGTGATATCTCAGCTTTGCTAGTACAGAGAGCTGAGAGATCTGGATATAAAGCAATTGTCCTGACTGCTGATACTCCCCGGCTAGGTAGAAGGGAGGCTGACATAAAGAACAAGTAGCAATGCTTAACATCTTAGTTTCTTTCCTCTCTCATGCATTGCATTCCTTTTGTTGGGCATAAAGTGCTTCATGGAATTGGGATAAGATACATCGTCGTATCATAACTCTTTAAGTCGTGGGATAGGATGTTTTAAGTCATCAAATTTTTAAACAGTGCCTTGGTTTTGTTTCATCTGGAATGGGACTTTGATGAACTTGCAGGATGATTACACCTCCACTGAAGAACCTTGAAGGCCTGTTGTCTGTTGAAGTTGGCTCTGTGAGTAATATTTAACTTCCCTATTTCGGAATCTTGGAGATGCCAGTCTTAATAGAATGATTTCCAATCTCTCTAATGTCCACCGTATGGTATAAGATTGATTGTGTGCCTGCCATTTAATCTGGATAAAGTAAATTTAACTCATGATCTCATATGCAGGATAAAGGTTCAATATTAGAGTCTTTTGTTAACGAGGCTTTTGATGCATCTCTTTGCTGGGAAGTACGTGCAATAAACAGTTCAGATATATATGCATACTTGATTGTCTGCGCTTATGAAAATTTTACTTTGATGCATGATAAGTTTTGAAGGGCATCCCTTTTGTAACTCGATAACTTAACTATTTCTATGTTATATAAATGGCTTCTACAGCATACCAGAAGTTTTAGTAATATCATCTCTTAATGAATTTTTTTACTGCTTAAAAGTTGTCTTTCTTATGTTTGATTTTACTGGCTCAGTTATGATATTTACCGTCCACAGGATATAGGGTGGTTAAAATCAATCACTAGCTTGCCAATTCTGATAAAGGGGATTCTCACTCACGAAGATGGTAAGATTAGAATTATGTCTTAAAGATATTTGAATTGAAATGAACCTTCTATTATCTCTATCAGATTAATGTTCTGAAATAACATATTTTACATTGTATTCAAATACTGGAAGTACGAACTCATTTTATCTGTCAATTAAGCTTCTGATATGATGGCTGCAGGAAAATGCATGATAATGTGTCTGGTTACCCTTTTTTTTGGCTTGATTAGTCCCTTGTTCTGGTTTGTGAGTTATGTTAGTGTCCATGGATAGCCCATTTAATTAGTTAACATGGTTGATGAATGGCAGCAATTAAGGCTGTGGAAGTAGGCGTTGATGGAATTATTGTCTCCAATCATGGAGCCCGCCAACTAGATTTTTCTCCTGCTACTGTTTCCGTCCTTGAAGAGGTAAATCTCATTTTGCACTATTTTTTTATTACGCGAAGCTGTCGGTACGAGGAAATATAATTATTCTTGAATTTCTGCGTAGATTAAATGTTCGGTACCTAACTCTTTTGAAACGTACCTTCTTAAGGGATTGTAAATGACTCCCAGTATCTGTATTGCTCTTGTTTCTCTTTAATTTATAGCTTTTAGACAGATATCACTGCATCCAACTGTTCCTTCACAACTTACTTTGCTCGTGTTGCTACATTCTCTCCTTTACTTGCACATTCTTTCAAAAACTTTGGTGAATTTACACAAGTTTGTCTTGTATGATGCATTTTCCATATTACTTGATATAGGAAAGCAATATGTGAATGTGGCTCTTGTTAGGGCCATAGAATCAGAACCAAGCATACCAAGTGTAGTTGCCCTCAAGGGACAGCGCAGTTGGCAAGTGCTTGGGGTCTCTTGGTCATACTTACTGGCTTAGAGGTCTCAAGTTCGAACCTTCTGGCGAGCTTAATATCAAAAACCTATGATGTCTCCTAGGTCTGGCCCTTGAGGCGGGCACGGGTGCCCCTAGGTATAAGGGAGCAAAGTTTCGACTATCGGTTACCAAGTAGTTGAAATGTTGAATTGCATCACAGTATTATGCTTACTTTTAACAGGAAGTTTGATGCAGGTTGTTCATGCTGTCAAGGGTAAAGTTCCTGTCCTCTTAGATGGAGGTGTACGACGAGGAACAGATGTGTTTAAGGCGTTAGCTCTTGGTGCCCAAGCAGTCCTTGTAAGCTCTATACTCCCAACTCTATCCATACAAAACATTTGAAGTCCCATAATACATTTTCGTTTCAAGTTTCAAATGATTACTACTATTTACAGACAAAGGTCTCCAAATTTTATTCTGTGCTATTTGGTATTTAGTCGCCATGCTCTTCAAACTCACTCAATTGGCCTTTTGGGCCATGTGTGATAAAATCCTTGTTTCTTACTTTCAACTCTTCCTCTCAGAAGTATTTCCAAGAACACTGGGTTTTCTTTTTGTTTTTGTTTTTTTTTTTTTTACTCAAACCCTCGTAATATAGAACTAAATTAAATAGAATACATAGTAACTATCAAAAAATTAAATACAATTATGGTATAAATTAGGTTTAAATACTATTTTGATCCTTGTATTTTTTGCTTTGGTCCATGTTGGTTCTTGTAATTTCAAAACTTCTAGTTTGGTCTTTGTACTTTCAACTTTTGTTCATTTTAGTCTCTGTAAACGTCTCTTTTAGCTACTATACTTTTAAAAAGGGACCATTTTGGTCCCTTTACTTTCATTGTTATCTCATTTATTTTTACCATAATTTTTTTAACAGGACACTTCACTCACTAAATTTCTTGAAATGTAGCCATAAATTTTATTAAAGAGTTTCCATTGTGTTAAAATTTTGCATATCAGGACCAAATTGGACACTTATTAAAAGTACTGGACCAAAACGTATCATTTAATAGTACAAGACTAAAATAAACAAAAGTAGAAAATACAGGGACTAAAAAGAACGTTTTGAAAGTAAGTAAAGATGACTAAAATGAACCAAAGCCAAAAGTACATAGATGGTTTTCCATATACAAAAACACGAGCAATTACGAAACCGATATTTCTTCCCTTCCAAATTTTATGAAATGAAAGCCAAAAAAAAAATAGGTAGCCGAACTCAATTCCTTCAAGTAAATCCAGAAGAAGAAGCATTGTGAAATGAAGTCTTAGTTAACTTGTTCGTCTAACTTGTGACTGGAATGATTGGCTGATTCAGATAGGGAGGCCAGTTATATATGGGCTTGCAGCAAAGGGAGAAGAGGGAGTAAGAGCAGTGCTGGAAATGCTGAAGAATGAGTTAGAGATCACCATGGCTCTTTCTGGTTGCTCATCTATCAAGGACATTACTAGAAGCCTTGTAAGGACACACTATGATCATAACTTGCAATCAATGCTTTAATGTGTTTGCTGATTTACAAAACCAAAGGGCTCAGACTTCTGCAAGGATGCATCTCCAAATTCAGTAATGTTATTCCAATATTTGGCCACTTGGAGACATGGCTTTGTAATCCATGCCCAAAGTTTGGGCTAGTTAGAGCATGTGTGTCGTTGAAGGGGGAACAATGAACATGTATATGTATGTATGTACGTATGAGGTTTTAATGCTGTATCTTGTCTTGGTAATTTTCATCTTCATTTCACCATGACATCCAACAACTTACAAAACATGTAAATGTTGTTTAGAGGGTTGGAGAATGACTAACAATTAAGTTATAAGAGGTAATGAGAAGATGTGTGCTTCTTTTCAGCTTTCTATATATTTATAAAGTCAAAAGTGTGTGTCCTTCAATAAAGAAAAAAAATTCGGAAAATATTTGACTCCTTACCGGTAAGGAC

mRNA sequence

CTCTCGACTTATGTTGTGATGGGGAATACTTTTCTTCAAAAAAATTGAACGGTCTACTGTGTTTTACTCTAGAAATTTGCATCCTTTGATAAATTCTTCTCGTTTTTCCCCATAACAATGTAAAATTTTTACTATCTATTAATAGTTAAGAAAATACAATAGCATTTTTAAGTTCGACGCTTTCAATCCCCATCGGAGTAAATAAATTGCATATAAGTTCATCTAAAGATCGACTTCTAGTAGGTCCAATAATTAGCAATATGCCTTTCAAAAAAATAAAAAATAAATAATTGGCAACATGTCTTCAACTTTTAGTTGAACTTATGTTTTATTCAAGTTGGTGCTCTCGTAGAGCTAACCAGCTTGACCCATTCTGTATTGAAGACTGCACGGGTCAAAATAAGAGCGTCAAGTTAATAATTGATGTCAGCTCTGCCTTATCCATTGGCTCACTGTTTTTCCTCAATTTCAACATCCTCCTGGTGATATCTGATAAAATGTCATCTGAACCAGTGAATGTGAATGACTATAAAGAATTGGCTAGGCAAGCACTTCCAAAAATGTATTATGATTACTATGCGGGAGGAGCTGAGGACCAGCACACACTTAGAGAAAACATGCAAGCTTTCCATAGAATCATTATCCGACCTCGAATTCTTGTCGATGTAAGCAAAATTGATATGTCAACAACCATATTGGGCTACCTCATATCTGAACCTATCATGGTTGCTCCAACTGGTGCACACAAATTGGCACACTGTGAAGGAGAGCTAGCCACAGCAAGGGCAGCAGCAGCTGCAAAAACCATAATGGTTCTATCATATGCCTCATCCTACTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTTCGTTTTTTTCAATTATATGTTTTCAGAAAGCGTGATATCTCAGCTTTGCTAGTACAGAGAGCTGAGAGATCTGGATATAAAGCAATTGTCCTGACTGCTGATACTCCCCGGCTAGGTAGAAGGGAGGCTGACATAAAGAACAAGATGATTACACCTCCACTGAAGAACCTTGAAGGCCTGTTGTCTGTTGAAGTTGGCTCTGATAAAGGTTCAATATTAGAGTCTTTTGTTAACGAGGCTTTTGATGCATCTCTTTGCTGGGAAGATATAGGGTGGTTAAAATCAATCACTAGCTTGCCAATTCTGATAAAGGGGATTCTCACTCACGAAGATGCAATTAAGGCTGTGGAAGTAGGCGTTGATGGAATTATTGTCTCCAATCATGGAGCCCGCCAACTAGATTTTTCTCCTGCTACTGTTTCCGTCCTTGAAGAGGTTGTTCATGCTGTCAAGGGTAAAGTTCCTGTCCTCTTAGATGGAGGTGTACGACGAGGAACAGATGTGTTTAAGGCGTTAGCTCTTGGTGCCCAAGCAGTCCTTATAGGGAGGCCAGTTATATATGGGCTTGCAGCAAAGGGAGAAGAGGGAGTAAGAGCAGTGCTGGAAATGCTGAAGAATGAGTTAGAGATCACCATGGCTCTTTCTGGTTGCTCATCTATCAAGGACATTACTAGAAGCCTTGTAAGGACACACTATGATCATAACTTGCAATCAATGCTTTAATGTGTTTGCTGATTTACAAAACCAAAGGGCTCAGACTTCTGCAAGGATGCATCTCCAAATTCAGTAATGTTATTCCAATATTTGGCCACTTGGAGACATGGCTTTGTAATCCATGCCCAAAGTTTGGGCTAGTTAGAGCATGTGTGTCGTTGAAGGGGGAACAATGAACATGTATATGTATGTATGTACGTATGAGGTTTTAATGCTGTATCTTGTCTTGGTAATTTTCATCTTCATTTCACCATGACATCCAACAACTTACAAAACATGTAAATGTTGTTTAGAGGGTTGGAGAATGACTAACAATTAAGTTATAAGAGGTAATGAGAAGATGTGTGCTTCTTTTCAGCTTTCTATATATTTATAAAGTCAAAAGTGTGTGTCCTTCAATAAAGAAAAAAAATTCGGAAAATATTTGACTCCTTACCGGTAAGGAC

Coding sequence (CDS)

ATGTTTTATTCAAGTTGGTGCTCTCGTAGAGCTAACCAGCTTGACCCATTCTGTATTGAAGACTGCACGGGTCAAAATAAGAGCGTCAAGTTAATAATTGATGTCAGCTCTGCCTTATCCATTGGCTCACTGTTTTTCCTCAATTTCAACATCCTCCTGGTGATATCTGATAAAATGTCATCTGAACCAGTGAATGTGAATGACTATAAAGAATTGGCTAGGCAAGCACTTCCAAAAATGTATTATGATTACTATGCGGGAGGAGCTGAGGACCAGCACACACTTAGAGAAAACATGCAAGCTTTCCATAGAATCATTATCCGACCTCGAATTCTTGTCGATGTAAGCAAAATTGATATGTCAACAACCATATTGGGCTACCTCATATCTGAACCTATCATGGTTGCTCCAACTGGTGCACACAAATTGGCACACTGTGAAGGAGAGCTAGCCACAGCAAGGGCAGCAGCAGCTGCAAAAACCATAATGGTTCTATCATATGCCTCATCCTACTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTTCGTTTTTTTCAATTATATGTTTTCAGAAAGCGTGATATCTCAGCTTTGCTAGTACAGAGAGCTGAGAGATCTGGATATAAAGCAATTGTCCTGACTGCTGATACTCCCCGGCTAGGTAGAAGGGAGGCTGACATAAAGAACAAGATGATTACACCTCCACTGAAGAACCTTGAAGGCCTGTTGTCTGTTGAAGTTGGCTCTGATAAAGGTTCAATATTAGAGTCTTTTGTTAACGAGGCTTTTGATGCATCTCTTTGCTGGGAAGATATAGGGTGGTTAAAATCAATCACTAGCTTGCCAATTCTGATAAAGGGGATTCTCACTCACGAAGATGCAATTAAGGCTGTGGAAGTAGGCGTTGATGGAATTATTGTCTCCAATCATGGAGCCCGCCAACTAGATTTTTCTCCTGCTACTGTTTCCGTCCTTGAAGAGGTTGTTCATGCTGTCAAGGGTAAAGTTCCTGTCCTCTTAGATGGAGGTGTACGACGAGGAACAGATGTGTTTAAGGCGTTAGCTCTTGGTGCCCAAGCAGTCCTTATAGGGAGGCCAGTTATATATGGGCTTGCAGCAAAGGGAGAAGAGGGAGTAAGAGCAGTGCTGGAAATGCTGAAGAATGAGTTAGAGATCACCATGGCTCTTTCTGGTTGCTCATCTATCAAGGACATTACTAGAAGCCTTGTAAGGACACACTATGATCATAACTTGCAATCAATGCTTTAA

Protein sequence

MFYSSWCSRRANQLDPFCIEDCTGQNKSVKLIIDVSSALSIGSLFFLNFNILLVISDKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML
Homology
BLAST of MC08g1128 vs. ExPASy Swiss-Prot
Match: Q9LJH5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=GLO4 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 4.6e-148
Identity = 259/360 (71.94%), Postives = 320/360 (88.89%), Query Frame = 0

Query: 64  VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 123
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 124 ILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 183
           +LGY IS PIM+APT  HKLAH +GE+ATA+AAAA  TIM++S+ S+ +IEEVASSCNAV
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 184 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 243
           RF Q+YV+++RD++A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 244 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 303
           +S EV  ++GS +E+F + AFDASL W+DI WL+SIT LPIL+KG+LT EDA+KAVE GV
Sbjct: 185 VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGV 244

Query: 304 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 363
           DGI+VSNHGARQLD+SPAT++VLEEVVHAVKG++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 364 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 423
           IGRP++YGLAAKGE+GV+ V++MLKNE EITMALSGC +I D+TR+ VRT  +  ++SML
Sbjct: 305 IGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTE-NERIKSML 363

BLAST of MC08g1128 vs. ExPASy Swiss-Prot
Match: Q24JJ8 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=GLO3 PE=2 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.3e-147
Identity = 264/360 (73.33%), Postives = 312/360 (86.67%), Query Frame = 0

Query: 64  VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 123
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 124 ILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 183
           ILGY IS PIM+APTG HKLAH EGE ATA+AAAA  TIM++SY SS + EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 184 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 243
           RF Q+YV+++RDI+A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 244 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 303
            S EV   KGS +++F + AFDAS  W+DI WL+SIT LPIL+KGILT EDA+KAVE GV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 304 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 363
           DGIIVSNHG RQLD+SPAT++VLEEVV  V+G++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 364 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 423
           IGRP+IYGLAAKGE+GV+ V++MLKNE EITMALSGC +I DITR+ VRT  +  L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE-NERLHSML 363

BLAST of MC08g1128 vs. ExPASy Swiss-Prot
Match: Q8H3I4 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLO4 PE=1 SV=2)

HSP 1 Score: 466.8 bits (1200), Expect = 2.5e-130
Identity = 238/362 (65.75%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 63  PVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMST 122
           PVNV +Y+ELA++ALPKM YDY  GGAED+HTLREN+ A+ RII+RPR+LVDVSKIDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 123 TILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNA 182
           T+LGY +  PI+VAPTG HKLAH EGE ATARAAA+   IMVLS++SS  IE+VASSCNA
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 183 VRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEG 242
           +RF+QLYV++ R++SA LV+RAE  G+KA++LT DTP LGRREADI+NKM+ P   NLEG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 243 LLSV-EVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEV 302
           L++  +  +  GS LE F     D SL W+DI WLKSITS+PI +KGI+T EDA +AVE 
Sbjct: 186 LMTTDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEA 245

Query: 303 GVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQA 362
           GV G+IVSNHGARQLD++PAT++ LEEVV AV G VPVL+DGG+RRGTDVFKALALGA+A
Sbjct: 246 GVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARA 305

Query: 363 VLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQS 422
           V++GRPV +GLAA+GE G R V+EML  ELE+ MAL GC S+ +ITRS V T  D  ++S
Sbjct: 306 VMVGRPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGD-RIRS 365

Query: 423 ML 424
           +L
Sbjct: 366 LL 366

BLAST of MC08g1128 vs. ExPASy Swiss-Prot
Match: B8B8K5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=39946 GN=GLO4 PE=3 SV=2)

HSP 1 Score: 461.5 bits (1186), Expect = 1.1e-128
Identity = 236/362 (65.19%), Postives = 292/362 (80.66%), Query Frame = 0

Query: 63  PVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMST 122
           PVNV +Y+ELA++ALPKM YDY  GGAED+HTLREN+ A+ RII+RPR+LVDVSKIDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 123 TILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNA 182
           T+LGY +  PI+VAPTG HKLAH EGE ATARAAA+   IMVLS++SS  IE+VASSCNA
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 183 VRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEG 242
           +RF+QLYV++ R++SA LV+RAE  G+KA++LT DTP LGRREADI+NKM+ P   NLEG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 243 LLSV-EVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEV 302
           L+++ +  +  GS LE F     D SL W+DI WLKSITS+PI +KGI+T EDA +AVE 
Sbjct: 186 LMTIDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEA 245

Query: 303 GVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQA 362
           GV G+IVSNHGARQLD++PAT++ LEEVV AV G VPVL+DGG+RRGTDVFKALALGA+A
Sbjct: 246 GVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARA 305

Query: 363 VLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQS 422
           V+   PV +GLAA+GE G R V+EML  ELE+ MAL GC S+ +ITRS V T  D  ++S
Sbjct: 306 VMXXXPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGD-RIRS 365

Query: 423 ML 424
           +L
Sbjct: 366 LL 366

BLAST of MC08g1128 vs. ExPASy Swiss-Prot
Match: P05414 (Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.6e-119
Identity = 221/358 (61.73%), Postives = 278/358 (77.65%), Query Frame = 0

Query: 62  EPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMS 121
           E  NVN+Y+ +A+Q LPKM YDYYA GAEDQ TL EN  AF RI+ RPRIL+DV+ IDM+
Sbjct: 2   EITNVNEYEAIAKQKLPKMVYDYYASGAEDQWTLAENRNAFSRILFRPRILIDVTNIDMT 61

Query: 122 TTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCN 181
           TTILG+ IS PIM+APT   K+AH EGE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTILGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 182 AVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP---LK 241
            +RFFQLYV++ R++ A LV+RAER+G+KAI LT DTPRLGRREADIKN+ + PP   LK
Sbjct: 122 GIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLK 181

Query: 242 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 301
           N EG+   ++     S L S+V    D SL W+D+ WL++ITSLPIL+KG++T EDA  A
Sbjct: 182 NFEGIDLGKMDKANDSGLSSYVAGQIDRSLSWKDVAWLQTITSLPILVKGVITAEDARLA 241

Query: 302 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 361
           V+ G  GIIVSNHGARQLD+ PAT+  LEEVV A +G++PV LDGGVRRGTDVFKALALG
Sbjct: 242 VQHGAAGIIVSNHGARQLDYVPATIMALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 362 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 417
           A  V IGRPV++ LAA+GE GV+ VL+M+++E E+TMALSGC S+K+I+RS +   +D
Sbjct: 302 AAGVFIGRPVVFSLAAEGEAGVKKVLQMMRDEFELTMALSGCRSLKEISRSHIAADWD 359

BLAST of MC08g1128 vs. NCBI nr
Match: XP_022148211.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Momordica charantia])

HSP 1 Score: 707 bits (1825), Expect = 2.04e-255
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 0

Query: 57  DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS 116
           DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS
Sbjct: 8   DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS 67

Query: 117 KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV 176
           KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV
Sbjct: 68  KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV 127

Query: 177 ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP 236
           ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP
Sbjct: 128 ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP 187

Query: 237 LKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI 296
           LKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI
Sbjct: 188 LKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI 247

Query: 297 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 356
           KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA
Sbjct: 248 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 307

Query: 357 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 416
           LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD
Sbjct: 308 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 367

Query: 417 HNLQSML 423
           HNLQSML
Sbjct: 368 HNLQSML 374

BLAST of MC08g1128 vs. NCBI nr
Match: XP_022148212.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia] >XP_022148213.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia] >XP_022148214.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia] >XP_022148215.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia])

HSP 1 Score: 703 bits (1814), Expect = 6.90e-254
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 59  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 118
           MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI
Sbjct: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60

Query: 119 DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 178
           DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS
Sbjct: 61  DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120

Query: 179 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 238
           SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180

Query: 239 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 298
           NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA
Sbjct: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240

Query: 299 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 358
           VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300

Query: 359 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 418
           AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN
Sbjct: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360

Query: 419 LQSML 423
           LQSML
Sbjct: 361 LQSML 365

BLAST of MC08g1128 vs. NCBI nr
Match: XP_022148216.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X3 [Momordica charantia])

HSP 1 Score: 652 bits (1681), Expect = 8.01e-234
Identity = 346/367 (94.28%), Postives = 346/367 (94.28%), Query Frame = 0

Query: 57  DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS 116
           DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS
Sbjct: 8   DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS 67

Query: 117 KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV 176
           KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV
Sbjct: 68  KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV 127

Query: 177 ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP 236
           ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP
Sbjct: 128 ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP 187

Query: 237 LKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI 296
           LKNLEGLLSVEVGSD                     IGWLKSITSLPILIKGILTHEDAI
Sbjct: 188 LKNLEGLLSVEVGSD---------------------IGWLKSITSLPILIKGILTHEDAI 247

Query: 297 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 356
           KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA
Sbjct: 248 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 307

Query: 357 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 416
           LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD
Sbjct: 308 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 353

Query: 417 HNLQSML 423
           HNLQSML
Sbjct: 368 HNLQSML 353

BLAST of MC08g1128 vs. NCBI nr
Match: XP_038894778.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida] >XP_038894784.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida] >XP_038894792.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida] >XP_038894801.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 631 bits (1627), Expect = 2.17e-225
Identity = 327/367 (89.10%), Postives = 350/367 (95.37%), Query Frame = 0

Query: 59  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 118
           MS+EPVNV+D+KELARQALPKMYYDYYAGGAEDQHTLR+NMQAF+RI+I+PRILVDVSKI
Sbjct: 1   MSTEPVNVDDFKELARQALPKMYYDYYAGGAEDQHTLRQNMQAFYRIMIQPRILVDVSKI 60

Query: 119 DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 178
           DMSTTILGY IS PIMVAPTGAHKLA+ EGELATARAAAAAKTIMVLSYASSYS+EEVA+
Sbjct: 61  DMSTTILGYRISAPIMVAPTGAHKLAYYEGELATARAAAAAKTIMVLSYASSYSMEEVAT 120

Query: 179 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 238
           SCNA+RFFQLYVF++R+IS LLVQRAER GYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 121 SCNAIRFFQLYVFKRREISTLLVQRAERFGYKAIVLTADTPRLGRREADIKNKMITPPLK 180

Query: 239 NLEGLLSV--EVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI 298
           NLEGLLSV  EV SDKGS+LESFVNEAFD+SLCW+DI WLKSITSLP+L+KGILTHEDAI
Sbjct: 181 NLEGLLSVKIEVDSDKGSLLESFVNEAFDSSLCWKDIAWLKSITSLPVLVKGILTHEDAI 240

Query: 299 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 358
           KAVEVGVDGIIVSNHGARQLDFSP T+SV+EEVVHAVKGKVPVLLDGGVRRGTDVFKALA
Sbjct: 241 KAVEVGVDGIIVSNHGARQLDFSPPTISVVEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 300

Query: 359 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 418
           LGAQAVL+GRPVIYGLAAKGEEGVR VLEMLKNELEI+MALSGC  IKDITRS VRTHYD
Sbjct: 301 LGAQAVLVGRPVIYGLAAKGEEGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTHYD 360

Query: 419 HNLQSML 423
             L SML
Sbjct: 361 -KLHSML 366

BLAST of MC08g1128 vs. NCBI nr
Match: XP_022148294.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Momordica charantia] >XP_022148295.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Momordica charantia] >XP_022148296.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Momordica charantia])

HSP 1 Score: 608 bits (1569), Expect = 1.36e-216
Identity = 315/365 (86.30%), Postives = 341/365 (93.42%), Query Frame = 0

Query: 59  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 118
           MSSEP+NVND+KELARQALPKMYYD+YAGGAEDQHTLREN+QAFH+I IRPR+LVDVSKI
Sbjct: 1   MSSEPMNVNDFKELARQALPKMYYDFYAGGAEDQHTLRENIQAFHKITIRPRVLVDVSKI 60

Query: 119 DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 178
           DMSTTILGY IS PIMVAPT AHKLA+ EGELATARAAAAAKTIMVLSY+++ SIEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTAAHKLAYHEGELATARAAAAAKTIMVLSYSATSSIEEVAS 120

Query: 179 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 238
           SC+AVRFFQLYVF++RDI+ LLV+RAERSGYKAIVLTADTPRLGRREADIKNKMI PP+K
Sbjct: 121 SCDAVRFFQLYVFKRRDITTLLVKRAERSGYKAIVLTADTPRLGRREADIKNKMIAPPVK 180

Query: 239 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 298
           NLEGL+SVE+ SD+GS LE+  N   DASL WEDIGWL+SITSLPILIKGILTHEDA +A
Sbjct: 181 NLEGLVSVEINSDQGSKLEAHANATLDASLRWEDIGWLRSITSLPILIKGILTHEDATRA 240

Query: 299 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 358
           VE GVDGIIVSNHGARQLDF+PATVSVLEEVVHAVKG+VPVL DGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHAVKGRVPVLFDGGVRRGTDVFKALALG 300

Query: 359 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 418
           AQAVLIGRPV++GLAAKGEEGVR VLEMLKNELEITMALSGC SIKDITRSLVRT YD  
Sbjct: 301 AQAVLIGRPVLFGLAAKGEEGVRTVLEMLKNELEITMALSGCPSIKDITRSLVRTQYD-K 360

Query: 419 LQSML 423
           LQSML
Sbjct: 361 LQSML 364

BLAST of MC08g1128 vs. ExPASy TrEMBL
Match: A0A6J1D4P5 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 SV=1)

HSP 1 Score: 707 bits (1825), Expect = 9.88e-256
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 0

Query: 57  DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS 116
           DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS
Sbjct: 8   DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS 67

Query: 117 KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV 176
           KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV
Sbjct: 68  KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV 127

Query: 177 ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP 236
           ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP
Sbjct: 128 ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP 187

Query: 237 LKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI 296
           LKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI
Sbjct: 188 LKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI 247

Query: 297 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 356
           KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA
Sbjct: 248 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 307

Query: 357 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 416
           LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD
Sbjct: 308 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 367

Query: 417 HNLQSML 423
           HNLQSML
Sbjct: 368 HNLQSML 374

BLAST of MC08g1128 vs. ExPASy TrEMBL
Match: A0A6J1D2A9 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 SV=1)

HSP 1 Score: 703 bits (1814), Expect = 3.34e-254
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 59  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 118
           MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI
Sbjct: 1   MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 60

Query: 119 DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 178
           DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS
Sbjct: 61  DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 120

Query: 179 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 238
           SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK
Sbjct: 121 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 180

Query: 239 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 298
           NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA
Sbjct: 181 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 240

Query: 299 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 358
           VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 300

Query: 359 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 418
           AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN
Sbjct: 301 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 360

Query: 419 LQSML 423
           LQSML
Sbjct: 361 LQSML 365

BLAST of MC08g1128 vs. ExPASy TrEMBL
Match: A0A6J1D4Q2 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 SV=1)

HSP 1 Score: 652 bits (1681), Expect = 3.88e-234
Identity = 346/367 (94.28%), Postives = 346/367 (94.28%), Query Frame = 0

Query: 57  DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS 116
           DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS
Sbjct: 8   DKMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVS 67

Query: 117 KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV 176
           KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV
Sbjct: 68  KIDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEV 127

Query: 177 ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP 236
           ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP
Sbjct: 128 ASSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP 187

Query: 237 LKNLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAI 296
           LKNLEGLLSVEVGSD                     IGWLKSITSLPILIKGILTHEDAI
Sbjct: 188 LKNLEGLLSVEVGSD---------------------IGWLKSITSLPILIKGILTHEDAI 247

Query: 297 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 356
           KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA
Sbjct: 248 KAVEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALA 307

Query: 357 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 416
           LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD
Sbjct: 308 LGAQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 353

Query: 417 HNLQSML 423
           HNLQSML
Sbjct: 368 HNLQSML 353

BLAST of MC08g1128 vs. ExPASy TrEMBL
Match: A0A6J1D4Y8 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016982 PE=3 SV=1)

HSP 1 Score: 608 bits (1569), Expect = 6.60e-217
Identity = 315/365 (86.30%), Postives = 341/365 (93.42%), Query Frame = 0

Query: 59  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 118
           MSSEP+NVND+KELARQALPKMYYD+YAGGAEDQHTLREN+QAFH+I IRPR+LVDVSKI
Sbjct: 1   MSSEPMNVNDFKELARQALPKMYYDFYAGGAEDQHTLRENIQAFHKITIRPRVLVDVSKI 60

Query: 119 DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 178
           DMSTTILGY IS PIMVAPT AHKLA+ EGELATARAAAAAKTIMVLSY+++ SIEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTAAHKLAYHEGELATARAAAAAKTIMVLSYSATSSIEEVAS 120

Query: 179 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 238
           SC+AVRFFQLYVF++RDI+ LLV+RAERSGYKAIVLTADTPRLGRREADIKNKMI PP+K
Sbjct: 121 SCDAVRFFQLYVFKRRDITTLLVKRAERSGYKAIVLTADTPRLGRREADIKNKMIAPPVK 180

Query: 239 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 298
           NLEGL+SVE+ SD+GS LE+  N   DASL WEDIGWL+SITSLPILIKGILTHEDA +A
Sbjct: 181 NLEGLVSVEINSDQGSKLEAHANATLDASLRWEDIGWLRSITSLPILIKGILTHEDATRA 240

Query: 299 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 358
           VE GVDGIIVSNHGARQLDF+PATVSVLEEVVHAVKG+VPVL DGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHAVKGRVPVLFDGGVRRGTDVFKALALG 300

Query: 359 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 418
           AQAVLIGRPV++GLAAKGEEGVR VLEMLKNELEITMALSGC SIKDITRSLVRT YD  
Sbjct: 301 AQAVLIGRPVLFGLAAKGEEGVRTVLEMLKNELEITMALSGCPSIKDITRSLVRTQYD-K 360

Query: 419 LQSML 423
           LQSML
Sbjct: 361 LQSML 364

BLAST of MC08g1128 vs. ExPASy TrEMBL
Match: A0A1S3CCP5 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499183 PE=3 SV=1)

HSP 1 Score: 600 bits (1547), Expect = 1.47e-213
Identity = 307/365 (84.11%), Postives = 339/365 (92.88%), Query Frame = 0

Query: 59  MSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKI 118
           MSSEPVNVND+KELAR ALPKMYYD+Y+GGAED+HTLREN+QAF+RI IRPR+LVDVSK+
Sbjct: 1   MSSEPVNVNDFKELARLALPKMYYDFYSGGAEDEHTLRENIQAFYRITIRPRVLVDVSKV 60

Query: 119 DMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVAS 178
           DMSTTILG+ IS PI+VAPT AHKLA  EGELATARAAAA KTIMVLSY+S+ SIEEVAS
Sbjct: 61  DMSTTILGHHISAPILVAPTAAHKLAFHEGELATARAAAAVKTIMVLSYSSTCSIEEVAS 120

Query: 179 SCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLK 238
           SCN+VRFFQLY+F++RDISALLVQRAER GYKAI+LT DTPRLGRREADIKNKMI PP+K
Sbjct: 121 SCNSVRFFQLYIFKRRDISALLVQRAERCGYKAIILTVDTPRLGRREADIKNKMIAPPVK 180

Query: 239 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 298
           +LEGL+S++V SD+GS LE++ NE  DASL WEDIGWL+SIT+LPILIKG+LTHEDA KA
Sbjct: 181 SLEGLISIDVKSDQGSKLETYANEMLDASLSWEDIGWLRSITTLPILIKGVLTHEDATKA 240

Query: 299 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 358
           VE GVDGIIVSNHGARQLDF+PATVSVLEEVVHAVKGK+PVLLDGGVRRGTDVFKALALG
Sbjct: 241 VEAGVDGIIVSNHGARQLDFAPATVSVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 300

Query: 359 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHN 418
           AQAVLIGRPVIYGLAAKGEEGVR V+EMLKNELE +MALSGC SIKDITRS VRTHYD N
Sbjct: 301 AQAVLIGRPVIYGLAAKGEEGVRTVMEMLKNELETSMALSGCPSIKDITRSHVRTHYD-N 360

Query: 419 LQSML 423
           L SML
Sbjct: 361 LPSML 364

BLAST of MC08g1128 vs. TAIR 10
Match: AT3G14130.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 525.8 bits (1353), Expect = 3.2e-149
Identity = 259/360 (71.94%), Postives = 320/360 (88.89%), Query Frame = 0

Query: 64  VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 123
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 124 ILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 183
           +LGY IS PIM+APT  HKLAH +GE+ATA+AAAA  TIM++S+ S+ +IEEVASSCNAV
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 184 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 243
           RF Q+YV+++RD++A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 244 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 303
           +S EV  ++GS +E+F + AFDASL W+DI WL+SIT LPIL+KG+LT EDA+KAVE GV
Sbjct: 185 VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGV 244

Query: 304 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 363
           DGI+VSNHGARQLD+SPAT++VLEEVVHAVKG++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 364 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 423
           IGRP++YGLAAKGE+GV+ V++MLKNE EITMALSGC +I D+TR+ VRT  +  ++SML
Sbjct: 305 IGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTE-NERIKSML 363

BLAST of MC08g1128 vs. TAIR 10
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 524.2 bits (1349), Expect = 9.4e-149
Identity = 264/360 (73.33%), Postives = 312/360 (86.67%), Query Frame = 0

Query: 64  VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 123
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 124 ILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 183
           ILGY IS PIM+APTG HKLAH EGE ATA+AAAA  TIM++SY SS + EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 184 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 243
           RF Q+YV+++RDI+A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 244 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 303
            S EV   KGS +++F + AFDAS  W+DI WL+SIT LPIL+KGILT EDA+KAVE GV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 304 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 363
           DGIIVSNHG RQLD+SPAT++VLEEVV  V+G++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 364 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 423
           IGRP+IYGLAAKGE+GV+ V++MLKNE EITMALSGC +I DITR+ VRT  +  L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE-NERLHSML 363

BLAST of MC08g1128 vs. TAIR 10
Match: AT3G14150.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 524.2 bits (1349), Expect = 9.4e-149
Identity = 264/360 (73.33%), Postives = 312/360 (86.67%), Query Frame = 0

Query: 64  VNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMSTT 123
           VNV++++ELA+QALPKMYYD+Y GGAEDQHTL EN+QAF RI+ RPR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 124 ILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCNAV 183
           ILGY IS PIM+APTG HKLAH EGE ATA+AAAA  TIM++SY SS + EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 184 RFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPLKNLEGL 243
           RF Q+YV+++RDI+A +V+RAE++G+KAIVLT D PRLGRREADIKNKMI+P LKN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 244 LSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKAVEVGV 303
            S EV   KGS +++F + AFDAS  W+DI WL+SIT LPIL+KGILT EDA+KAVE GV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 304 DGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVL 363
           DGIIVSNHG RQLD+SPAT++VLEEVV  V+G++PVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 364 IGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYDHNLQSML 423
           IGRP+IYGLAAKGE+GV+ V++MLKNE EITMALSGC +I DITR+ VRT  +  L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE-NERLHSML 363

BLAST of MC08g1128 vs. TAIR 10
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 428.3 bits (1100), Expect = 7.0e-120
Identity = 220/358 (61.45%), Postives = 277/358 (77.37%), Query Frame = 0

Query: 62  EPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMS 121
           E  NV +Y  +A+Q LPKM YDYYA GAEDQ TL+EN  AF RI+ RPRIL+DVSKIDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 122 TTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCN 181
           TT+LG+ IS PIMVAPT   K+AH +GE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 182 AVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP---LK 241
            +RFFQLYV++ R++   LV+RAER+G+KAI LT DTPRLGRRE+DIKN+   PP   LK
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 242 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 301
           N EGL   ++     S L S+V    D +L W+D+ WL++IT LPIL+KG+LT EDA  A
Sbjct: 182 NFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIA 241

Query: 302 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 361
           ++ G  GIIVSNHGARQLD+ PAT+S LEEVV A +G++PV LDGGVRRGTDVFKALALG
Sbjct: 242 IQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 362 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 417
           A  + IGRPV++ LAA+GE GVR VL+ML++E E+TMALSGC S+K+I+R+ + T +D
Sbjct: 302 ASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWD 359

BLAST of MC08g1128 vs. TAIR 10
Match: AT3G14420.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 428.3 bits (1100), Expect = 7.0e-120
Identity = 220/358 (61.45%), Postives = 277/358 (77.37%), Query Frame = 0

Query: 62  EPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSKIDMS 121
           E  NV +Y  +A+Q LPKM YDYYA GAEDQ TL+EN  AF RI+ RPRIL+DVSKIDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 122 TTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVASSCN 181
           TT+LG+ IS PIMVAPT   K+AH +GE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 182 AVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPP---LK 241
            +RFFQLYV++ R++   LV+RAER+G+KAI LT DTPRLGRRE+DIKN+   PP   LK
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 242 NLEGLLSVEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDAIKA 301
           N EGL   ++     S L S+V    D +L W+D+ WL++IT LPIL+KG+LT EDA  A
Sbjct: 182 NFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIA 241

Query: 302 VEVGVDGIIVSNHGARQLDFSPATVSVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALG 361
           ++ G  GIIVSNHGARQLD+ PAT+S LEEVV A +G++PV LDGGVRRGTDVFKALALG
Sbjct: 242 IQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 362 AQAVLIGRPVIYGLAAKGEEGVRAVLEMLKNELEITMALSGCSSIKDITRSLVRTHYD 417
           A  + IGRPV++ LAA+GE GVR VL+ML++E E+TMALSGC S+K+I+R+ + T +D
Sbjct: 302 ASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWD 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LJH54.6e-14871.94Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q24JJ81.3e-14773.33Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8H3I42.5e-13065.75Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=3... [more]
B8B8K51.1e-12865.19Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=399... [more]
P054142.6e-11961.73Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea OX=3562 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022148211.12.04e-255100.00peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Momordica charantia... [more]
XP_022148212.16.90e-254100.00peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Momordica charantia... [more]
XP_022148216.18.01e-23494.28peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X3 [Momordica charantia... [more]
XP_038894778.12.17e-22589.10peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Benincasa hispida] ... [more]
XP_022148294.11.36e-21686.30peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like [Momordica charantia] >XP_02214... [more]
Match NameE-valueIdentityDescription
A0A6J1D4P59.88e-256100.00(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 S... [more]
A0A6J1D2A93.34e-254100.00(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 S... [more]
A0A6J1D4Q23.88e-23494.28(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 S... [more]
A0A6J1D4Y86.60e-21786.30(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016982 PE=3 S... [more]
A0A1S3CCP51.47e-21384.11(S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499183 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14130.13.2e-14971.94Aldolase-type TIM barrel family protein [more]
AT3G14150.19.4e-14973.33Aldolase-type TIM barrel family protein [more]
AT3G14150.29.4e-14973.33Aldolase-type TIM barrel family protein [more]
AT3G14420.17.0e-12061.45Aldolase-type TIM barrel family protein [more]
AT3G14420.27.0e-12061.45Aldolase-type TIM barrel family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000262FMN-dependent dehydrogenasePFAMPF01070FMN_dhcoord: 73..412
e-value: 3.0E-134
score: 447.5
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 61..422
e-value: 6.6E-161
score: 537.1
IPR012133Alpha-hydroxy acid dehydrogenase, FMN-dependentPIRSFPIRSF000138Al-hdrx_acd_dhcoord: 52..416
e-value: 1.0E-136
score: 453.8
IPR012133Alpha-hydroxy acid dehydrogenase, FMN-dependentCDDcd02809alpha_hydroxyacid_oxid_FMNcoord: 67..408
e-value: 6.96794E-160
score: 450.746
NoneNo IPR availablePIRSRPIRSR000138-2PIRSR000138-2coord: 58..413
e-value: 3.9E-138
score: 458.2
NoneNo IPR availablePANTHERPTHR10578:SF116PEROXISOMAL (S)-2-HYDROXY-ACID OXIDASE GLO4-LIKEcoord: 59..413
NoneNo IPR availablePANTHERPTHR10578S -2-HYDROXY-ACID OXIDASE-RELATEDcoord: 59..413
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 63..413
IPR008259FMN-dependent alpha-hydroxy acid dehydrogenase, active sitePROSITEPS00557FMN_HYDROXY_ACID_DH_1coord: 309..315
IPR037396FMN hydroxy acid dehydrogenase domainPROSITEPS51349FMN_HYDROXY_ACID_DH_2coord: 58..416
score: 112.659409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1128.1MC08g1128.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009854 oxidative photosynthetic carbon pathway
cellular_component GO:0005777 peroxisome
molecular_function GO:0010181 FMN binding
molecular_function GO:0003973 (S)-2-hydroxy-acid oxidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016491 oxidoreductase activity