MC08g1035 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1035 vs. ExPASy Swiss-Prot
Match: Q9CA89 (Rop guanine nucleotide exchange factor 12 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF12 PE=1 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.7e-163
Identity = 342/558 (61.29%), Postives = 401/558 (71.86%), Query Frame = 0

Query: 1   MVREME-EEENHRSKLFYFKMQ--DNNSAFCFLQIIMILFFNRSQSSVPLNDTVLEKPPT 60
           MVR  E E+E +RS+LF FK +  DNNSA             R   S+ + +T L++  T
Sbjct: 1   MVRASEQEQETYRSRLFNFKWRNNDNNSA------------TRHNKSLSV-ETGLDEAAT 60

Query: 61  NRQDK-----------APKAKVCRDDAA----APVKPPKDQPPTEIEQMKERFAKLLLGE 120
              D             P ++   D+A     A  +  + Q   ++EQMKERF+KLLLGE
Sbjct: 61  GSHDAEPLTIIHPSQGPPLSRSAADEAVLAALAASQARERQLLADMEQMKERFSKLLLGE 120

Query: 121 DMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVE 180
           D SGGGKGVSSALALSNAITNLAASVFGEQ RLEPM  ER+ARWRKEID LLSVTDY+VE
Sbjct: 121 DNSGGGKGVSSALALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLSVTDYVVE 180

Query: 181 FVASQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSS 240
           F  SQQK+KDGTNMEIM TRQR DLHMNIPAL+KLDAMLIDCL+NFKDQSEF Y+S+DS 
Sbjct: 181 FAPSQQKNKDGTNMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFSYISKDSP 240

Query: 241 DANKDNASKRSDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEME 300
           D    +  +  +KWW+PT KVP +GLSE SR+F+QYQKDCVNQVLKAAMAINAQVL EME
Sbjct: 241 DL---DGKRNDEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINAQVLFEME 300

Query: 301 IPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVI 360
           IPE+YI+SLPKNGR SLGD +Y+NITV+FFDPDQFLS+MD+SSEHKI+DLK+RIEASI+I
Sbjct: 301 IPESYIDSLPKNGRASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNRIEASIII 360

Query: 361 WKRKMNQKDGKSA--WGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNR 420
           WKRKM  KD KS+  W S VSLEKRE+FEERAETILLILK R+PG  QS+LDISKIQ+N 
Sbjct: 361 WKRKMVYKDNKSSAPWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDISKIQFNE 420

Query: 421 DVGQAVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKKRETPSVSPAEETQNA 480
           DVGQAVLESYSRILESLA+TV+SRI+DVL AD         +  KR TP +   EET   
Sbjct: 421 DVGQAVLESYSRILESLAYTVLSRIDDVLEAD--------RAGNKRNTP-LEAEEET--- 480

Query: 481 AETPTSMTLLDFMNWGPDLGDN---EVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNK 536
                SMTL DFM W  D   N   E KK+ P         P ++ K      ++VT  K
Sbjct: 481 --LVGSMTLSDFMGWDFDQAANAELESKKDLP-------DDPLIKEK-----LSVVTTKK 515

BLAST of MC08g1035 vs. ExPASy Swiss-Prot
Match: Q9M811 (Rop guanine nucleotide exchange factor 11 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF11 PE=1 SV=2)

HSP 1 Score: 567.0 bits (1460), Expect = 2.3e-160
Identity = 335/563 (59.50%), Postives = 398/563 (70.69%), Query Frame = 0

Query: 1   MVREME-EEENHRSKLFYFKMQDNNSAF---------CFLQI-----------IMILFFN 60
           MV+ ME E+E ++S+LF+FK  + NSA          C +++            M++F  
Sbjct: 1   MVKAMEQEQETYKSRLFHFKNMNENSASRHVKSWSSDCAMRMDGSDNLDDDDNDMMMF-- 60

Query: 61  RSQ----SSVPLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERF 120
           RSQ     SV      +     NR DK P+        A  +          +EQMKE+F
Sbjct: 61  RSQPGKCGSVDRPSLPIGGVTPNRNDKLPRVSSSDSMEALIIL------QAAMEQMKEKF 120

Query: 121 AKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLS 180
           +KLLLGEDMSGGGKGVSSALALSNAITNLAAS FGEQ RLEPM+V+RK RWR+EI  L+S
Sbjct: 121 SKLLLGEDMSGGGKGVSSALALSNAITNLAASAFGEQRRLEPMAVDRKTRWRREIGWLIS 180

Query: 181 VTDYIVEFVASQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFY 240
           V DYIVEF  +QQ +KDGT+ME+M TRQR DL  NIPAL+KLDAML+DCLD FKDQ EFY
Sbjct: 181 VADYIVEFAPTQQTNKDGTSMEVMSTRQRTDLLCNIPALKKLDAMLLDCLDKFKDQDEFY 240

Query: 241 YVSRDSSDANKDNASKRSDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINA 300
           YV +DS D+ +   ++  +KWWLP  KVP NGLSE SR+F+Q QK+CVNQVLKAAMAINA
Sbjct: 241 YVKKDSPDSCE---TRNDEKWWLPAVKVPPNGLSEISRRFLQSQKECVNQVLKAAMAINA 300

Query: 301 QVLSEMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDR 360
           QVLSEMEIPE+Y+ESLPKNGR SLGD IYR ITVE FD DQFL  MDLSSEHKILDLK+R
Sbjct: 301 QVLSEMEIPESYLESLPKNGRASLGDVIYRMITVEMFDADQFLIEMDLSSEHKILDLKNR 360

Query: 361 IEASIVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISK 420
           IEASIVIWKRKM QKD KS WGS VS+EKRE FEERAETILL+LK  FPG  QS+LDISK
Sbjct: 361 IEASIVIWKRKMVQKDTKSPWGSTVSIEKREQFEERAETILLLLKQGFPGISQSSLDISK 420

Query: 421 IQYNRDVGQAVLESYSRILESLAFTVMSRIEDVLYADYIAQNP-SQASSKKRET--PSVS 480
           IQ+NRDVG A+LESYSR+LESLA TVMSRIEDVLYAD + Q P + A SK R +   +  
Sbjct: 421 IQFNRDVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSLKENEK 480

Query: 481 PAEETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNI 536
             EE  +  E   S TL D M WG    +NE+KKES  G+         E   + K+  I
Sbjct: 481 LREERLSFTEDMASGTLSDVMQWGNK--NNEMKKESFFGDR--------EKPLLSKVTGI 540

BLAST of MC08g1035 vs. ExPASy Swiss-Prot
Match: Q9LV40 (Rho guanine nucleotide exchange factor 8 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF8 PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.9e-151
Identity = 311/539 (57.70%), Postives = 378/539 (70.13%), Query Frame = 0

Query: 13  SKLFYFKMQDNNSAFCFLQIIMILFFNRSQSSVPLNDTVLEKPPTNRQDKAPKAKVCRDD 72
           SK F FK   ++S+    Q   I+  N     V  N      P +   D   ++ V   +
Sbjct: 12  SKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESN-----TPESQNSDSFVESPV---E 71

Query: 73  AAAPVKPPKDQP-------PTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLA 132
           ++ P+  P  +P         ++E MK+RFAKLLLGEDMSGGGKGVSSALALSNAITNLA
Sbjct: 72  SSLPMISPLTRPGKRSERQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALALSNAITNLA 131

Query: 133 ASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMVTRQRN 192
           AS+FGEQ +L+PM  +R+ARW+KEID LLSVTD+IVEFV SQQ SKDG   EIMVTRQR 
Sbjct: 132 ASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCTEIMVTRQRG 191

Query: 193 DLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPTPKVPT 252
           DL MNIPALRKLDAMLID LDNF+  +EF+YVSRDS +  +    + +DKWWLP  KVP 
Sbjct: 192 DLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKWWLPPVKVPP 251

Query: 253 NGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLGDSIYR 312
            GLSE SR+ + +QKD V QV KAAMAINAQVLSEMEIPE+YI+SLPKNGR SLGDSIY+
Sbjct: 252 GGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGRASLGDSIYK 311

Query: 313 NITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWGSAVSLEKR 372
           +IT E+FDP+QFL+ +D+S+EHK+LDLK+RIEAS+VIWKRK++ KD KS+WGSAVSLEKR
Sbjct: 312 SITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSWGSAVSLEKR 371

Query: 373 ELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFTVMSRI 432
           ELFEERAETIL++LK +FPG PQS+LDISKIQ+N+DVGQAVLESYSRILESLA+TVMSRI
Sbjct: 372 ELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILESLAYTVMSRI 431

Query: 433 EDVLYADYIAQNPS----QASSKKRETPSVSPAEETQNAAE-----TPTSMTLLDFMNWG 492
           EDVLY D +A   +    + S   R T + S +  + N+ E      P S TLLDFM W 
Sbjct: 432 EDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSKTLLDFMGW- 491

Query: 493 PDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 536
               DN  K     G      SP +  K +  L+           LEN+ G +SP  RH
Sbjct: 492 ---NDNSSK-----GGDKPTKSPNLTPKKLSYLEK----------LENLNGFRSPKDRH 523

BLAST of MC08g1035 vs. ExPASy Swiss-Prot
Match: Q9SVQ3 (Rop guanine nucleotide exchange factor 9 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF9 PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-150
Identity = 300/514 (58.37%), Postives = 367/514 (71.40%), Query Frame = 0

Query: 40  RSQSSVPLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLL 99
           + Q    L +T + +  T         +  R   +  +   +D+  +E E MK+RF KLL
Sbjct: 27  KEQQQPHLAETTMPESQTQDSLGGSPVETSRPMTSRLISRRQDKQQSETEMMKDRFTKLL 86

Query: 100 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 159
           LGEDMSGGGKGVSSALALSNAITNLAAS+FGEQ +L+PM+ +R+ARW+KEID LLSVTD+
Sbjct: 87  LGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQPMAPDRRARWKKEIDWLLSVTDH 146

Query: 160 IVEFVASQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSR 219
           IVEFV SQQ SK+G   EIMVTRQR DL MNIPALRKLDAMLID LDNF+  +EF+YVSR
Sbjct: 147 IVEFVPSQQISKEGVCTEIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSR 206

Query: 220 DSSDANKDNASKRSDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLS 279
           DS +  +    +  DKWWLP  KVP NGLSE++R+ + +QKD V+QV KAAMAINAQVLS
Sbjct: 207 DSEEGKQARNERTKDKWWLPPVKVPPNGLSESARRMLHFQKDSVSQVQKAAMAINAQVLS 266

Query: 280 EMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEAS 339
           EM IP++YIESLPKNGR SLGDS+Y++IT E+FDP+QFLST+DLS+EHK+LD+K+RIEAS
Sbjct: 267 EMAIPDSYIESLPKNGRVSLGDSLYKSITEEWFDPEQFLSTLDLSTEHKVLDVKNRIEAS 326

Query: 340 IVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYN 399
           IVIWKRK++ KD KS+WGSAVSLEKRELFEERAETIL++LK +FPG PQS+LDISKIQYN
Sbjct: 327 IVIWKRKLHLKDNKSSWGSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQYN 386

Query: 400 RDVGQAVLESYSRILESLAFTVMSRIEDVLYADYIA------QNPSQASSKKRETPSVSP 459
           +DVG AVLESYSRILESL +T MSRI+DVLYAD +A      +  S       ET S S 
Sbjct: 387 KDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARKQCTGEETSDGGKIATETDSASA 446

Query: 460 AE-----------ETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAP-SPEV 519
                        E+QN+++T    TLLDF+ W     DN  K     G S+  P SP +
Sbjct: 447 GSSNYSGEEIEKLESQNSSKT----TLLDFIGW----SDNSSK-----GQSEKPPKSPRM 506

Query: 520 EMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 536
             K +  L+           LEN+ G +SP  RH
Sbjct: 507 TPKKLSYLEK----------LENLNGFRSPKDRH 517

BLAST of MC08g1035 vs. ExPASy Swiss-Prot
Match: Q1KS66 (Rop guanine nucleotide exchange factor 10 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF10 PE=2 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 7.8e-137
Identity = 271/468 (57.91%), Postives = 342/468 (73.08%), Query Frame = 0

Query: 73  AAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQ 132
           AA   +  K    +++E MKERFAKLLLGEDMSGGG G +SALALSNAIT LA S+FGEQ
Sbjct: 30  AAPSTRRGKQNRRSDMEVMKERFAKLLLGEDMSGGGTGETSALALSNAITKLADSMFGEQ 89

Query: 133 WRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMVTRQRNDLHMNIP 192
            +L+PM  E K  WRKE+  LLSV D+IV+FV S+Q  K+G   EIMVT+QR+DL  NIP
Sbjct: 90  MKLQPMYPETKENWRKEMGWLLSVIDHIVQFVPSRQMGKNGQFTEIMVTKQRDDLLTNIP 149

Query: 193 ALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPTPKVPTNGLSENS 252
           ALRKLD++L++ LDNFKDQ +F+YV RD  DA+ +   +R + WWLP  KVPT+GLSE S
Sbjct: 150 ALRKLDSVLLETLDNFKDQKDFWYVPRDMEDADHNGDWRRDENWWLPVVKVPTDGLSEES 209

Query: 253 RKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLGDSIYRNITVEFF 312
           R+++Q QKD V QVLKAA AINA VLSEM +PENYI+SLPKNG+ SLGD +Y++IT E F
Sbjct: 210 RRWLQNQKDSVAQVLKAATAINAHVLSEMHVPENYIDSLPKNGKTSLGDFLYKSITEESF 269

Query: 313 DPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKM--NQKDGKSAWGSAVSLEKRELFEE 372
           DPD F+S +DLS+EHK+LDLK+RIEAS+VIWKRKM   +KDGKS WGS VSLEKRELFE 
Sbjct: 270 DPDYFVSFLDLSTEHKVLDLKNRIEASMVIWKRKMCQKEKDGKSQWGSTVSLEKRELFEV 329

Query: 373 RAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFTVMSRIEDVLY 432
           RAETIL++LK +FPG PQS+L++SKI+ N+DVGQA+LESYSR+LESLA  +MSRIEDVL 
Sbjct: 330 RAETILVMLKQQFPGIPQSSLEVSKIKNNKDVGQAILESYSRVLESLASKIMSRIEDVLE 389

Query: 433 ADYIAQNPSQASSKKR-ETPSVSPAEETQN--AAETPTSMTLLDFMNWGPDLGDNEVKKE 492
           AD + Q      ++ R E+ + S  EET+   AAETP S  L DF+ W      ++ KK 
Sbjct: 390 ADRLVQRQLMGEAETRSESEAESEYEETEKVVAAETPNSRKLSDFIGWRL---SSDTKKH 449

Query: 493 SPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 536
           S   + +     E E K    +++     K  SYL  +  ++SP+ RH
Sbjct: 450 SSMSDIEFFHKVEQE-KEKPMMKSPRALPKKFSYLAKLENMRSPSDRH 493

BLAST of MC08g1035 vs. NCBI nr
Match: XP_022153663.1 (rop guanine nucleotide exchange factor 12-like [Momordica charantia])

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 507/535 (94.77%), Postives = 507/535 (94.77%), Query Frame = 0

Query: 1   MVREMEEEENHRSKLFYFKMQDNNSAFCFLQIIMILFFNRSQSSVPLNDTVLEKPPTNRQ 60
           MVREMEEEENHRS                            QSSVPLNDTVLEKPPTNRQ
Sbjct: 1   MVREMEEEENHRS----------------------------QSSVPLNDTVLEKPPTNRQ 60

Query: 61  DKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNA 120
           DKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNA
Sbjct: 61  DKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNA 120

Query: 121 ITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMV 180
           ITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMV
Sbjct: 121 ITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMV 180

Query: 181 TRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPT 240
           TRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPT
Sbjct: 181 TRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPT 240

Query: 241 PKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLG 300
           PKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLG
Sbjct: 241 PKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLG 300

Query: 301 DSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWGSAV 360
           DSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWGSAV
Sbjct: 301 DSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWGSAV 360

Query: 361 SLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFT 420
           SLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFT
Sbjct: 361 SLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFT 420

Query: 421 VMSRIEDVLYADYIAQNPSQASSKKRETPSVSPAEETQNAAETPTSMTLLDFMNWGPDLG 480
           VMSRIEDVLYADYIAQNPSQASSKKRETPSVSPAEETQNAAETPTSMTLLDFMNWGPDLG
Sbjct: 421 VMSRIEDVLYADYIAQNPSQASSKKRETPSVSPAEETQNAAETPTSMTLLDFMNWGPDLG 480

Query: 481 DNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 535
           DNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH
Sbjct: 481 DNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 507

BLAST of MC08g1035 vs. NCBI nr
Match: XP_038902752.1 (rop guanine nucleotide exchange factor 12-like [Benincasa hispida])

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 485/560 (86.61%), Postives = 502/560 (89.64%), Query Frame = 0

Query: 1   MVREMEE-EENHRSKLFYFK-MQDNNSAFCFLQIIMI-------------LFFNRSQSSV 60
           MVREME+ EEN+RSKLF FK  Q+NN+A    + + +             LF NR+Q S 
Sbjct: 1   MVREMEQDEENYRSKLFNFKAFQENNNATKHSKSLSMESATVLFSSDNNYLFSNRNQGST 60

Query: 61  PLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMS 120
           P+NDT+ +KPPTNRQDK PKAKV RDDAA  +K PKDQPPTEIEQMKERFAKLLLGEDMS
Sbjct: 61  PVNDTISDKPPTNRQDKGPKAKVSRDDAA--IKQPKDQPPTEIEQMKERFAKLLLGEDMS 120

Query: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVA 180
           GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFV 
Sbjct: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVP 180

Query: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDAN 240
           SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSS++N
Sbjct: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSESN 240

Query: 241 KDNASKRSD-KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300
           KDNASKR D KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP
Sbjct: 241 KDNASKRKDDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300

Query: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360
           ENYIE LPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK
Sbjct: 301 ENYIECLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360

Query: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420
           RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQS LDISKIQYNRDVGQ
Sbjct: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSGLDISKIQYNRDVGQ 420

Query: 421 AVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKK---RETPSVS------PAE 480
           AVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQAS KK   RETP  S        E
Sbjct: 421 AVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASGKKNPSRETPVSSGVDKCTTGE 480

Query: 481 ETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTN 535
           ETQNAAETPTSMTLLDFMNWGPD+ DNEVKKESPT NSD   S E E+KHVHKLQNIVTN
Sbjct: 481 ETQNAAETPTSMTLLDFMNWGPDIADNEVKKESPTANSDNL-STESEVKHVHKLQNIVTN 540

BLAST of MC08g1035 vs. NCBI nr
Match: XP_008447513.1 (PREDICTED: rop guanine nucleotide exchange factor 12-like isoform X1 [Cucumis melo])

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 483/560 (86.25%), Postives = 503/560 (89.82%), Query Frame = 0

Query: 1   MVREMEE-EENHRSKLFYFK-MQDNNSAFCFLQIIMI-------------LFFNRSQSSV 60
           MVREME+ EEN+RSKLF+FK MQ+NN+A    + + +             LF NRSQ S 
Sbjct: 1   MVREMEQDEENYRSKLFHFKAMQENNNATKHSKSLSMESASVLFSSDNNDLFSNRSQGST 60

Query: 61  PLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMS 120
           P++D + EKPP +RQDK PKAK+ RDDAA  +K PKDQPPTEIEQMKERFAKLLLGEDMS
Sbjct: 61  PISDNIAEKPPMSRQDKGPKAKLSRDDAA--IKQPKDQPPTEIEQMKERFAKLLLGEDMS 120

Query: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVA 180
           GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFV 
Sbjct: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVP 180

Query: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDAN 240
           SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDS+D+N
Sbjct: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSNDSN 240

Query: 241 KDNASKRSD-KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300
           KDNASKR D KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP
Sbjct: 241 KDNASKRKDDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300

Query: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360
           ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK
Sbjct: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360

Query: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420
           RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ
Sbjct: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420

Query: 421 AVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKK---RETPSVS------PAE 480
           AVLESYSRILESLAFTVMSRIEDVLYADY+AQNPSQAS KK   RE P  S      P E
Sbjct: 421 AVLESYSRILESLAFTVMSRIEDVLYADYVAQNPSQASGKKNPSRENPVSSGTDKFPPGE 480

Query: 481 ETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTN 535
           ETQNAAETPTSMTLLDFMNWGPD+ DNEVKKESP  NSD   S E E+KHVHKLQNIVTN
Sbjct: 481 ETQNAAETPTSMTLLDFMNWGPDIADNEVKKESPPANSDNL-STEGEVKHVHKLQNIVTN 540

BLAST of MC08g1035 vs. NCBI nr
Match: XP_011651499.1 (rop guanine nucleotide exchange factor 12 isoform X1 [Cucumis sativus] >KAE8650730.1 hypothetical protein Csa_023418 [Cucumis sativus])

HSP 1 Score: 900 bits (2325), Expect = 0.0
Identity = 480/560 (85.71%), Postives = 501/560 (89.46%), Query Frame = 0

Query: 1   MVREMEE-EENHRSKLFYFK-MQDNNSAFCFLQIIMI-------------LFFNRSQSSV 60
           MVREME+ EEN+RSKLF+FK MQ+NN+A    + + +             LF NRSQ S 
Sbjct: 1   MVREMEQDEENYRSKLFHFKAMQENNNATKHSKSLSMESASVLFSSDNNDLFSNRSQGST 60

Query: 61  PLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMS 120
           P +D V +KPP NRQDK PKAK+ RDDAA  +K PKDQPPTE EQMKERFAKLLLGEDMS
Sbjct: 61  PTSDNVSDKPPMNRQDKGPKAKLSRDDAA--IKQPKDQPPTETEQMKERFAKLLLGEDMS 120

Query: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVA 180
           GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFV 
Sbjct: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVP 180

Query: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDAN 240
           SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDS+++N
Sbjct: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSNESN 240

Query: 241 KDNASKRSD-KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300
           KDNASKR D KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP
Sbjct: 241 KDNASKRKDDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300

Query: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360
           ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK
Sbjct: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360

Query: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420
           RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ
Sbjct: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420

Query: 421 AVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKK---RETPSVS------PAE 480
           AVLESYSRILESLAFTVMSRIEDVLYADY+ QNPSQAS KK   RETP  S      P E
Sbjct: 421 AVLESYSRILESLAFTVMSRIEDVLYADYVTQNPSQASGKKNPSRETPVSSGTDKFPPGE 480

Query: 481 ETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTN 535
           ETQNAAETPTSMTLLDFMNWGPD+ D+E KKESP  NSD  P+ E E+KHVHKLQNIVTN
Sbjct: 481 ETQNAAETPTSMTLLDFMNWGPDIADSEAKKESPPANSDNLPT-EGEVKHVHKLQNIVTN 540

BLAST of MC08g1035 vs. NCBI nr
Match: XP_008447514.1 (PREDICTED: rop guanine nucleotide exchange factor 12-like isoform X2 [Cucumis melo])

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 459/506 (90.71%), Postives = 471/506 (93.08%), Query Frame = 0

Query: 40  RSQSSVPLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLL 99
           RSQ S P++D + EKPP +RQDK PKAK+ RDDAA  +K PKDQPPTEIEQMKERFAKLL
Sbjct: 13  RSQGSTPISDNIAEKPPMSRQDKGPKAKLSRDDAA--IKQPKDQPPTEIEQMKERFAKLL 72

Query: 100 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 159
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY
Sbjct: 73  LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 132

Query: 160 IVEFVASQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSR 219
           IVEFV SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSR
Sbjct: 133 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSR 192

Query: 220 DSSDANKDNASKRSD-KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVL 279
           DS+D+NKDNASKR D KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVL
Sbjct: 193 DSNDSNKDNASKRKDDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVL 252

Query: 280 SEMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEA 339
           SEMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEA
Sbjct: 253 SEMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEA 312

Query: 340 SIVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQY 399
           SIVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQY
Sbjct: 313 SIVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQY 372

Query: 400 NRDVGQAVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKK---RETPSVS--- 459
           NRDVGQAVLESYSRILESLAFTVMSRIEDVLYADY+AQNPSQAS KK   RE P  S   
Sbjct: 373 NRDVGQAVLESYSRILESLAFTVMSRIEDVLYADYVAQNPSQASGKKNPSRENPVSSGTD 432

Query: 460 ---PAEETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKL 519
              P EETQNAAETPTSMTLLDFMNWGPD+ DNEVKKESP  NSD   S E E+KHVHKL
Sbjct: 433 KFPPGEETQNAAETPTSMTLLDFMNWGPDIADNEVKKESPPANSDNL-STEGEVKHVHKL 492

Query: 520 QNIVTNNKLVSYLENIGGLKSPTSRH 535
           QNIVTNNKLVSYLENIGGLKSPT+RH
Sbjct: 493 QNIVTNNKLVSYLENIGGLKSPTARH 515

BLAST of MC08g1035 vs. ExPASy TrEMBL
Match: A0A6J1DI32 (rop guanine nucleotide exchange factor 12-like OS=Momordica charantia OX=3673 GN=LOC111021117 PE=4 SV=1)

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 507/535 (94.77%), Postives = 507/535 (94.77%), Query Frame = 0

Query: 1   MVREMEEEENHRSKLFYFKMQDNNSAFCFLQIIMILFFNRSQSSVPLNDTVLEKPPTNRQ 60
           MVREMEEEENHRS                            QSSVPLNDTVLEKPPTNRQ
Sbjct: 1   MVREMEEEENHRS----------------------------QSSVPLNDTVLEKPPTNRQ 60

Query: 61  DKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNA 120
           DKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNA
Sbjct: 61  DKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNA 120

Query: 121 ITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMV 180
           ITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMV
Sbjct: 121 ITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMV 180

Query: 181 TRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPT 240
           TRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPT
Sbjct: 181 TRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPT 240

Query: 241 PKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLG 300
           PKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLG
Sbjct: 241 PKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLG 300

Query: 301 DSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWGSAV 360
           DSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWGSAV
Sbjct: 301 DSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWGSAV 360

Query: 361 SLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFT 420
           SLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFT
Sbjct: 361 SLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFT 420

Query: 421 VMSRIEDVLYADYIAQNPSQASSKKRETPSVSPAEETQNAAETPTSMTLLDFMNWGPDLG 480
           VMSRIEDVLYADYIAQNPSQASSKKRETPSVSPAEETQNAAETPTSMTLLDFMNWGPDLG
Sbjct: 421 VMSRIEDVLYADYIAQNPSQASSKKRETPSVSPAEETQNAAETPTSMTLLDFMNWGPDLG 480

Query: 481 DNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 535
           DNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH
Sbjct: 481 DNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 507

BLAST of MC08g1035 vs. ExPASy TrEMBL
Match: A0A1S3BII1 (rop guanine nucleotide exchange factor 12-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489945 PE=4 SV=1)

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 483/560 (86.25%), Postives = 503/560 (89.82%), Query Frame = 0

Query: 1   MVREMEE-EENHRSKLFYFK-MQDNNSAFCFLQIIMI-------------LFFNRSQSSV 60
           MVREME+ EEN+RSKLF+FK MQ+NN+A    + + +             LF NRSQ S 
Sbjct: 1   MVREMEQDEENYRSKLFHFKAMQENNNATKHSKSLSMESASVLFSSDNNDLFSNRSQGST 60

Query: 61  PLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMS 120
           P++D + EKPP +RQDK PKAK+ RDDAA  +K PKDQPPTEIEQMKERFAKLLLGEDMS
Sbjct: 61  PISDNIAEKPPMSRQDKGPKAKLSRDDAA--IKQPKDQPPTEIEQMKERFAKLLLGEDMS 120

Query: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVA 180
           GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFV 
Sbjct: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVP 180

Query: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDAN 240
           SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDS+D+N
Sbjct: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSNDSN 240

Query: 241 KDNASKRSD-KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300
           KDNASKR D KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP
Sbjct: 241 KDNASKRKDDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300

Query: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360
           ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK
Sbjct: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360

Query: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420
           RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ
Sbjct: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420

Query: 421 AVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKK---RETPSVS------PAE 480
           AVLESYSRILESLAFTVMSRIEDVLYADY+AQNPSQAS KK   RE P  S      P E
Sbjct: 421 AVLESYSRILESLAFTVMSRIEDVLYADYVAQNPSQASGKKNPSRENPVSSGTDKFPPGE 480

Query: 481 ETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTN 535
           ETQNAAETPTSMTLLDFMNWGPD+ DNEVKKESP  NSD   S E E+KHVHKLQNIVTN
Sbjct: 481 ETQNAAETPTSMTLLDFMNWGPDIADNEVKKESPPANSDNL-STEGEVKHVHKLQNIVTN 540

BLAST of MC08g1035 vs. ExPASy TrEMBL
Match: A0A1S3BH13 (rop guanine nucleotide exchange factor 12-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489945 PE=4 SV=1)

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 459/506 (90.71%), Postives = 471/506 (93.08%), Query Frame = 0

Query: 40  RSQSSVPLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLL 99
           RSQ S P++D + EKPP +RQDK PKAK+ RDDAA  +K PKDQPPTEIEQMKERFAKLL
Sbjct: 13  RSQGSTPISDNIAEKPPMSRQDKGPKAKLSRDDAA--IKQPKDQPPTEIEQMKERFAKLL 72

Query: 100 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 159
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY
Sbjct: 73  LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 132

Query: 160 IVEFVASQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSR 219
           IVEFV SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSR
Sbjct: 133 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSR 192

Query: 220 DSSDANKDNASKRSD-KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVL 279
           DS+D+NKDNASKR D KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVL
Sbjct: 193 DSNDSNKDNASKRKDDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVL 252

Query: 280 SEMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEA 339
           SEMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEA
Sbjct: 253 SEMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEA 312

Query: 340 SIVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQY 399
           SIVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQY
Sbjct: 313 SIVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQY 372

Query: 400 NRDVGQAVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKK---RETPSVS--- 459
           NRDVGQAVLESYSRILESLAFTVMSRIEDVLYADY+AQNPSQAS KK   RE P  S   
Sbjct: 373 NRDVGQAVLESYSRILESLAFTVMSRIEDVLYADYVAQNPSQASGKKNPSRENPVSSGTD 432

Query: 460 ---PAEETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKL 519
              P EETQNAAETPTSMTLLDFMNWGPD+ DNEVKKESP  NSD   S E E+KHVHKL
Sbjct: 433 KFPPGEETQNAAETPTSMTLLDFMNWGPDIADNEVKKESPPANSDNL-STEGEVKHVHKL 492

Query: 520 QNIVTNNKLVSYLENIGGLKSPTSRH 535
           QNIVTNNKLVSYLENIGGLKSPT+RH
Sbjct: 493 QNIVTNNKLVSYLENIGGLKSPTARH 515

BLAST of MC08g1035 vs. ExPASy TrEMBL
Match: A0A5A7U6A7 (Rop guanine nucleotide exchange factor 12-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold404G00390 PE=4 SV=1)

HSP 1 Score: 870 bits (2249), Expect = 1.37e-315
Identity = 470/560 (83.93%), Postives = 490/560 (87.50%), Query Frame = 0

Query: 1   MVREMEE-EENHRSKLFYFK-MQDNNSAFCFLQIIMI-------------LFFNRSQSSV 60
           MVREME+ EEN+RSKLF+FK MQ+NN+A    + + +             LF NRSQ S 
Sbjct: 1   MVREMEQDEENYRSKLFHFKAMQENNNATKHSKSLSMESASVLFSSDNNDLFSNRSQGST 60

Query: 61  PLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMS 120
           P++D + EKPP +RQDK PKAK+ RDDAA  +K PKDQPPTEIEQMKERFAKLLLGEDMS
Sbjct: 61  PISDNIAEKPPMSRQDKGPKAKLSRDDAA--IKQPKDQPPTEIEQMKERFAKLLLGEDMS 120

Query: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVA 180
           GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEF  
Sbjct: 121 GGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEF-- 180

Query: 181 SQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDAN 240
                       IMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDS+D+N
Sbjct: 181 ------------IMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSNDSN 240

Query: 241 KDNASKRSD-KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300
           KDNASKR D KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP
Sbjct: 241 KDNASKRKDDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIP 300

Query: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360
           ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK
Sbjct: 301 ENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWK 360

Query: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420
           RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ
Sbjct: 361 RKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQ 420

Query: 421 AVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKK---RETPSVS------PAE 480
           AVLESYSRILESLAFTVMSRIEDVLYADY+AQNPSQAS KK   RE P  S      P E
Sbjct: 421 AVLESYSRILESLAFTVMSRIEDVLYADYVAQNPSQASGKKNPSRENPVSSGTDKFPPGE 480

Query: 481 ETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTN 535
           ETQNAAETPTSMTLLDFMNWGPD+ DNEVKKESP  NSD   S E E+KHVHKLQNIVTN
Sbjct: 481 ETQNAAETPTSMTLLDFMNWGPDIADNEVKKESPPANSDNL-STEGEVKHVHKLQNIVTN 540

BLAST of MC08g1035 vs. ExPASy TrEMBL
Match: A0A5D3CBI9 (Rop guanine nucleotide exchange factor 12-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G001010 PE=4 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 1.46e-313
Identity = 464/554 (83.75%), Postives = 485/554 (87.55%), Query Frame = 0

Query: 6   EEEENHRSKLFYFK-MQDNNSAFCFLQIIMI-------------LFFNRSQSSVPLNDTV 65
           ++EEN+RSKLF+FK MQ+NN+A    + + +             LF NRSQ S P++D +
Sbjct: 3   QDEENYRSKLFHFKAMQENNNATKHSKSLSMESASVLFSSDNNDLFSNRSQGSTPISDNI 62

Query: 66  LEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGV 125
            EKPP +RQDK PKAK+ RDDAA  +K PKDQPPTEIEQMKERFAKLLLGEDMSGGGKGV
Sbjct: 63  AEKPPMSRQDKGPKAKLSRDDAA--IKQPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGV 122

Query: 126 SSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSK 185
           SSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEF        
Sbjct: 123 SSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEF-------- 182

Query: 186 DGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASK 245
                 IMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDS+D+NKDNASK
Sbjct: 183 ------IMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSNDSNKDNASK 242

Query: 246 RSD-KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIES 305
           R D KWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIES
Sbjct: 243 RKDDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIES 302

Query: 306 LPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQK 365
           LPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQK
Sbjct: 303 LPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQK 362

Query: 366 DGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESY 425
           DGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESY
Sbjct: 363 DGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESY 422

Query: 426 SRILESLAFTVMSRIEDVLYADYIAQNPSQASSKK---RETPSVS------PAEETQNAA 485
           SRILESLAFTVMSRIEDVLYADY+AQNPSQAS KK   RE P  S      P EETQNAA
Sbjct: 423 SRILESLAFTVMSRIEDVLYADYVAQNPSQASGKKNPSRENPVSSGTDKFPPGEETQNAA 482

Query: 486 ETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSY 535
           ETPTSMTLLDFMNWGPD+ DNEVKKESP  NSD   S E E+KHVHKLQNIVTNNKLVSY
Sbjct: 483 ETPTSMTLLDFMNWGPDIADNEVKKESPPANSDNL-STEGEVKHVHKLQNIVTNNKLVSY 539

BLAST of MC08g1035 vs. TAIR 10
Match: AT1G79860.1 (RHO guanyl-nucleotide exchange factor 12 )

HSP 1 Score: 576.2 bits (1484), Expect = 2.6e-164
Identity = 342/558 (61.29%), Postives = 401/558 (71.86%), Query Frame = 0

Query: 1   MVREME-EEENHRSKLFYFKMQ--DNNSAFCFLQIIMILFFNRSQSSVPLNDTVLEKPPT 60
           MVR  E E+E +RS+LF FK +  DNNSA             R   S+ + +T L++  T
Sbjct: 1   MVRASEQEQETYRSRLFNFKWRNNDNNSA------------TRHNKSLSV-ETGLDEAAT 60

Query: 61  NRQDK-----------APKAKVCRDDAA----APVKPPKDQPPTEIEQMKERFAKLLLGE 120
              D             P ++   D+A     A  +  + Q   ++EQMKERF+KLLLGE
Sbjct: 61  GSHDAEPLTIIHPSQGPPLSRSAADEAVLAALAASQARERQLLADMEQMKERFSKLLLGE 120

Query: 121 DMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVE 180
           D SGGGKGVSSALALSNAITNLAASVFGEQ RLEPM  ER+ARWRKEID LLSVTDY+VE
Sbjct: 121 DNSGGGKGVSSALALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLSVTDYVVE 180

Query: 181 FVASQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSS 240
           F  SQQK+KDGTNMEIM TRQR DLHMNIPAL+KLDAMLIDCL+NFKDQSEF Y+S+DS 
Sbjct: 181 FAPSQQKNKDGTNMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFSYISKDSP 240

Query: 241 DANKDNASKRSDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEME 300
           D    +  +  +KWW+PT KVP +GLSE SR+F+QYQKDCVNQVLKAAMAINAQVL EME
Sbjct: 241 DL---DGKRNDEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINAQVLFEME 300

Query: 301 IPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVI 360
           IPE+YI+SLPKNGR SLGD +Y+NITV+FFDPDQFLS+MD+SSEHKI+DLK+RIEASI+I
Sbjct: 301 IPESYIDSLPKNGRASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNRIEASIII 360

Query: 361 WKRKMNQKDGKSA--WGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNR 420
           WKRKM  KD KS+  W S VSLEKRE+FEERAETILLILK R+PG  QS+LDISKIQ+N 
Sbjct: 361 WKRKMVYKDNKSSAPWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDISKIQFNE 420

Query: 421 DVGQAVLESYSRILESLAFTVMSRIEDVLYADYIAQNPSQASSKKRETPSVSPAEETQNA 480
           DVGQAVLESYSRILESLA+TV+SRI+DVL AD         +  KR TP +   EET   
Sbjct: 421 DVGQAVLESYSRILESLAYTVLSRIDDVLEAD--------RAGNKRNTP-LEAEEET--- 480

Query: 481 AETPTSMTLLDFMNWGPDLGDN---EVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNK 536
                SMTL DFM W  D   N   E KK+ P         P ++ K      ++VT  K
Sbjct: 481 --LVGSMTLSDFMGWDFDQAANAELESKKDLP-------DDPLIKEK-----LSVVTTKK 515

BLAST of MC08g1035 vs. TAIR 10
Match: AT1G52240.1 (RHO guanyl-nucleotide exchange factor 11 )

HSP 1 Score: 562.0 bits (1447), Expect = 5.2e-160
Identity = 313/481 (65.07%), Postives = 363/481 (75.47%), Query Frame = 0

Query: 58  NRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALAL 117
           NR DK P+        A  +          +EQMKE+F+KLLLGEDMSGGGKGVSSALAL
Sbjct: 145 NRNDKLPRVSSSDSMEALIIL------QAAMEQMKEKFSKLLLGEDMSGGGKGVSSALAL 204

Query: 118 SNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNME 177
           SNAITNLAAS FGEQ RLEPM+V+RK RWR+EI  L+SV DYIVEF  +QQ +KDGT+ME
Sbjct: 205 SNAITNLAASAFGEQRRLEPMAVDRKTRWRREIGWLISVADYIVEFAPTQQTNKDGTSME 264

Query: 178 IMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWW 237
           +M TRQR DL  NIPAL+KLDAML+DCLD FKDQ EFYYV +DS D+ +   ++  +KWW
Sbjct: 265 VMSTRQRTDLLCNIPALKKLDAMLLDCLDKFKDQDEFYYVKKDSPDSCE---TRNDEKWW 324

Query: 238 LPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRE 297
           LP  KVP NGLSE SR+F+Q QK+CVNQVLKAAMAINAQVLSEMEIPE+Y+ESLPKNGR 
Sbjct: 325 LPAVKVPPNGLSEISRRFLQSQKECVNQVLKAAMAINAQVLSEMEIPESYLESLPKNGRA 384

Query: 298 SLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWG 357
           SLGD IYR ITVE FD DQFL  MDLSSEHKILDLK+RIEASIVIWKRKM QKD KS WG
Sbjct: 385 SLGDVIYRMITVEMFDADQFLIEMDLSSEHKILDLKNRIEASIVIWKRKMVQKDTKSPWG 444

Query: 358 SAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESL 417
           S VS+EKRE FEERAETILL+LK  FPG  QS+LDISKIQ+NRDVG A+LESYSR+LESL
Sbjct: 445 STVSIEKREQFEERAETILLLLKQGFPGISQSSLDISKIQFNRDVGLAILESYSRVLESL 504

Query: 418 AFTVMSRIEDVLYADYIAQNP-SQASSKKRET--PSVSPAEETQNAAETPTSMTLLDFMN 477
           A TVMSRIEDVLYAD + Q P + A SK R +   +    EE  +  E   S TL D M 
Sbjct: 505 AHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSLKENEKLREERLSFTEDMASGTLSDVMQ 564

Query: 478 WGPDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSR 536
           WG    +NE+KKES  G+         E   + K+  I+TNNK  SYL+N+G ++SPT+R
Sbjct: 565 WGNK--NNEMKKESFFGDR--------EKPLLSKVTGIMTNNKKSSYLDNLGAMRSPTAR 606

BLAST of MC08g1035 vs. TAIR 10
Match: AT3G24620.1 (RHO guanyl-nucleotide exchange factor 8 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.4e-152
Identity = 311/539 (57.70%), Postives = 378/539 (70.13%), Query Frame = 0

Query: 13  SKLFYFKMQDNNSAFCFLQIIMILFFNRSQSSVPLNDTVLEKPPTNRQDKAPKAKVCRDD 72
           SK F FK   ++S+    Q   I+  N     V  N      P +   D   ++ V   +
Sbjct: 12  SKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESN-----TPESQNSDSFVESPV---E 71

Query: 73  AAAPVKPPKDQP-------PTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLA 132
           ++ P+  P  +P         ++E MK+RFAKLLLGEDMSGGGKGVSSALALSNAITNLA
Sbjct: 72  SSLPMISPLTRPGKRSERQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALALSNAITNLA 131

Query: 133 ASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMVTRQRN 192
           AS+FGEQ +L+PM  +R+ARW+KEID LLSVTD+IVEFV SQQ SKDG   EIMVTRQR 
Sbjct: 132 ASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCTEIMVTRQRG 191

Query: 193 DLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPTPKVPT 252
           DL MNIPALRKLDAMLID LDNF+  +EF+YVSRDS +  +    + +DKWWLP  KVP 
Sbjct: 192 DLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKWWLPPVKVPP 251

Query: 253 NGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLGDSIYR 312
            GLSE SR+ + +QKD V QV KAAMAINAQVLSEMEIPE+YI+SLPKNGR SLGDSIY+
Sbjct: 252 GGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGRASLGDSIYK 311

Query: 313 NITVEFFDPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKMNQKDGKSAWGSAVSLEKR 372
           +IT E+FDP+QFL+ +D+S+EHK+LDLK+RIEAS+VIWKRK++ KD KS+WGSAVSLEKR
Sbjct: 312 SITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSWGSAVSLEKR 371

Query: 373 ELFEERAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFTVMSRI 432
           ELFEERAETIL++LK +FPG PQS+LDISKIQ+N+DVGQAVLESYSRILESLA+TVMSRI
Sbjct: 372 ELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILESLAYTVMSRI 431

Query: 433 EDVLYADYIAQNPS----QASSKKRETPSVSPAEETQNAAE-----TPTSMTLLDFMNWG 492
           EDVLY D +A   +    + S   R T + S +  + N+ E      P S TLLDFM W 
Sbjct: 432 EDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSKTLLDFMGW- 491

Query: 493 PDLGDNEVKKESPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 536
               DN  K     G      SP +  K +  L+           LEN+ G +SP  RH
Sbjct: 492 ---NDNSSK-----GGDKPTKSPNLTPKKLSYLEK----------LENLNGFRSPKDRH 523

BLAST of MC08g1035 vs. TAIR 10
Match: AT4G13240.1 (RHO guanyl-nucleotide exchange factor 9 )

HSP 1 Score: 534.6 bits (1376), Expect = 8.8e-152
Identity = 300/514 (58.37%), Postives = 367/514 (71.40%), Query Frame = 0

Query: 40  RSQSSVPLNDTVLEKPPTNRQDKAPKAKVCRDDAAAPVKPPKDQPPTEIEQMKERFAKLL 99
           + Q    L +T + +  T         +  R   +  +   +D+  +E E MK+RF KLL
Sbjct: 27  KEQQQPHLAETTMPESQTQDSLGGSPVETSRPMTSRLISRRQDKQQSETEMMKDRFTKLL 86

Query: 100 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 159
           LGEDMSGGGKGVSSALALSNAITNLAAS+FGEQ +L+PM+ +R+ARW+KEID LLSVTD+
Sbjct: 87  LGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQPMAPDRRARWKKEIDWLLSVTDH 146

Query: 160 IVEFVASQQKSKDGTNMEIMVTRQRNDLHMNIPALRKLDAMLIDCLDNFKDQSEFYYVSR 219
           IVEFV SQQ SK+G   EIMVTRQR DL MNIPALRKLDAMLID LDNF+  +EF+YVSR
Sbjct: 147 IVEFVPSQQISKEGVCTEIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSR 206

Query: 220 DSSDANKDNASKRSDKWWLPTPKVPTNGLSENSRKFMQYQKDCVNQVLKAAMAINAQVLS 279
           DS +  +    +  DKWWLP  KVP NGLSE++R+ + +QKD V+QV KAAMAINAQVLS
Sbjct: 207 DSEEGKQARNERTKDKWWLPPVKVPPNGLSESARRMLHFQKDSVSQVQKAAMAINAQVLS 266

Query: 280 EMEIPENYIESLPKNGRESLGDSIYRNITVEFFDPDQFLSTMDLSSEHKILDLKDRIEAS 339
           EM IP++YIESLPKNGR SLGDS+Y++IT E+FDP+QFLST+DLS+EHK+LD+K+RIEAS
Sbjct: 267 EMAIPDSYIESLPKNGRVSLGDSLYKSITEEWFDPEQFLSTLDLSTEHKVLDVKNRIEAS 326

Query: 340 IVIWKRKMNQKDGKSAWGSAVSLEKRELFEERAETILLILKHRFPGTPQSALDISKIQYN 399
           IVIWKRK++ KD KS+WGSAVSLEKRELFEERAETIL++LK +FPG PQS+LDISKIQYN
Sbjct: 327 IVIWKRKLHLKDNKSSWGSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQYN 386

Query: 400 RDVGQAVLESYSRILESLAFTVMSRIEDVLYADYIA------QNPSQASSKKRETPSVSP 459
           +DVG AVLESYSRILESL +T MSRI+DVLYAD +A      +  S       ET S S 
Sbjct: 387 KDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARKQCTGEETSDGGKIATETDSASA 446

Query: 460 AE-----------ETQNAAETPTSMTLLDFMNWGPDLGDNEVKKESPTGNSDTAP-SPEV 519
                        E+QN+++T    TLLDF+ W     DN  K     G S+  P SP +
Sbjct: 447 GSSNYSGEEIEKLESQNSSKT----TLLDFIGW----SDNSSK-----GQSEKPPKSPRM 506

Query: 520 EMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 536
             K +  L+           LEN+ G +SP  RH
Sbjct: 507 TPKKLSYLEK----------LENLNGFRSPKDRH 517

BLAST of MC08g1035 vs. TAIR 10
Match: AT5G19560.1 (ROP uanine nucleotide exchange factor 10 )

HSP 1 Score: 488.8 bits (1257), Expect = 5.6e-138
Identity = 271/468 (57.91%), Postives = 342/468 (73.08%), Query Frame = 0

Query: 73  AAAPVKPPKDQPPTEIEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQ 132
           AA   +  K    +++E MKERFAKLLLGEDMSGGG G +SALALSNAIT LA S+FGEQ
Sbjct: 30  AAPSTRRGKQNRRSDMEVMKERFAKLLLGEDMSGGGTGETSALALSNAITKLADSMFGEQ 89

Query: 133 WRLEPMSVERKARWRKEIDLLLSVTDYIVEFVASQQKSKDGTNMEIMVTRQRNDLHMNIP 192
            +L+PM  E K  WRKE+  LLSV D+IV+FV S+Q  K+G   EIMVT+QR+DL  NIP
Sbjct: 90  MKLQPMYPETKENWRKEMGWLLSVIDHIVQFVPSRQMGKNGQFTEIMVTKQRDDLLTNIP 149

Query: 193 ALRKLDAMLIDCLDNFKDQSEFYYVSRDSSDANKDNASKRSDKWWLPTPKVPTNGLSENS 252
           ALRKLD++L++ LDNFKDQ +F+YV RD  DA+ +   +R + WWLP  KVPT+GLSE S
Sbjct: 150 ALRKLDSVLLETLDNFKDQKDFWYVPRDMEDADHNGDWRRDENWWLPVVKVPTDGLSEES 209

Query: 253 RKFMQYQKDCVNQVLKAAMAINAQVLSEMEIPENYIESLPKNGRESLGDSIYRNITVEFF 312
           R+++Q QKD V QVLKAA AINA VLSEM +PENYI+SLPKNG+ SLGD +Y++IT E F
Sbjct: 210 RRWLQNQKDSVAQVLKAATAINAHVLSEMHVPENYIDSLPKNGKTSLGDFLYKSITEESF 269

Query: 313 DPDQFLSTMDLSSEHKILDLKDRIEASIVIWKRKM--NQKDGKSAWGSAVSLEKRELFEE 372
           DPD F+S +DLS+EHK+LDLK+RIEAS+VIWKRKM   +KDGKS WGS VSLEKRELFE 
Sbjct: 270 DPDYFVSFLDLSTEHKVLDLKNRIEASMVIWKRKMCQKEKDGKSQWGSTVSLEKRELFEV 329

Query: 373 RAETILLILKHRFPGTPQSALDISKIQYNRDVGQAVLESYSRILESLAFTVMSRIEDVLY 432
           RAETIL++LK +FPG PQS+L++SKI+ N+DVGQA+LESYSR+LESLA  +MSRIEDVL 
Sbjct: 330 RAETILVMLKQQFPGIPQSSLEVSKIKNNKDVGQAILESYSRVLESLASKIMSRIEDVLE 389

Query: 433 ADYIAQNPSQASSKKR-ETPSVSPAEETQN--AAETPTSMTLLDFMNWGPDLGDNEVKKE 492
           AD + Q      ++ R E+ + S  EET+   AAETP S  L DF+ W      ++ KK 
Sbjct: 390 ADRLVQRQLMGEAETRSESEAESEYEETEKVVAAETPNSRKLSDFIGWRL---SSDTKKH 449

Query: 493 SPTGNSDTAPSPEVEMKHVHKLQNIVTNNKLVSYLENIGGLKSPTSRH 536
           S   + +     E E K    +++     K  SYL  +  ++SP+ RH
Sbjct: 450 SSMSDIEFFHKVEQE-KEKPMMKSPRALPKKFSYLAKLENMRSPSDRH 493