MC08g1004 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1004
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionTATA-binding protein-associated factor 2N-like
LocationMC08: 8133537 .. 8136002 (-)
RNA-Seq ExpressionMC08g1004
SyntenyMC08g1004
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGGTTTGGAAGCAAAGTATTGGCAATACTCCACTTTTTGAGTGGGATTTGGGTGTATATAAACGCCTCTTTCCCTTCTTTACCTATTCCATCGCCATTTCTTCATTCTTCATTCTTCATTCTTCACCATTACTGCACCACAGCAGCTCCTCTCCTTCACATTTCTCCTTCCGGTTTGTTCTCCATCTTCTTTTCTTTTTTTTATTAGAATTTTTTGAGAACCGTTGGATAATGTTTTCGTTTTTCCTTCTTTCCCCCTCTCTCATATCTCATATTCTTTAATTTGTTTAGAAAGAAAAAACAAAACTAGCTTCAATACTTGTTTCATGTGTTTTTTCTCCTTCTTTAATTCGTTTAAAGTTCGTTTGATGATGTTCTCGTTCTCAATTTCATTAAGTTTTAGGTTCTTTGTTTAAACATTCTTTAATTTGTATAGAAATTTTGTATCGTTATTGAATTTGTGGATTTTGGGTTCATGTAATTATATTTTTCTTTATTAAAAATAAGGAAACATGAACAAGAAACGAAAAATGTTACAAAATCGACCATTCGTTTCTTTTTTTTTTTCCTTTCTCTCTTTAATTTTACTTGAAAATTAACTTTTTTTAAAGTCAATTTTGTTTTGTTATATATATGTTTTTTATATTTAATTTATTTTGTAGGTAAAAATGATAAAGTATTGATAGAAAAAAGATTAAAAACAGAAACAAGAAAATCAAATGTTCTTTCTTACCAAATGTATATATAATTTTAAAAATGTTTTAGAAATTTTTTTCTCTCTTTATAAAAAAAATTTATCGGTAATCAGTATTTTCATATGTCAGTAAGAAAGATTTTTTATTTTAAACATAGTTCAACTGAGTCATGAGAATTCTCTAACTCTGGTATTAGAAAATAGTTTATCAAAAGTTACCAAAGAGCCCTCGGTTTCATTTGTATGTATAAAAGTTGGAAGTTTGAAATCCATTTGCAGAGAGAAGATGAGCAGACCAGGAGACTGGAATTGCAGGTCGTGCCAGCACTTGAACTTCCAGAGGAGGGACACGTGCCAGCGCTGCGGCGACCCCAAGAGCGGCGGCGCTGATTTTGGCGGTCTAATGAACGGCGGCGGAAGAGGAGGCTCCGCATTCGGCTACGGCGGCTCCGACGTCAGGCCCGGAGACTGGTACTGTACCGTCGGCAATTGTGGAGCCCACAATTTCGCTAGCCGCTCGAGCTGCTTCAAATGCGGTGCCTTCAAAGACGACATGTTGGGTGGAGGCTTCGACTTCGATCTTCCTCGCTCCAGAGGAACCTCCTTCGGCTTTGCCAATGGCACCGGTGCTGGCGGCCGAACTGGTGCCTCTGCCTGGAAGTCTGGAGACTGGATTTGTGCCCGGTAATATTTCAGGCCCGTTTCTATTTTTACAAACTAAAGTTGTAAACATTACTTTCTTTATTTTGCCTTTTAACTTTTAAAATAAAATTTAAAAACTAAGAAAACAGAAAGTTATTTTTACTTTTTAAATTTGGTTAAGAAATTAAATATTTTTTACTAAAGAAAGTGCGAGACTCAACCAACATCATTTTCAAAGTAGAAAACTAAAAACTAAAACAACTTTACCTCCTTGTGAGTAAAAAAGAAATTCTTATACTCGGTGACTATCGATTTTTTTTATCCAACAAAAATAAAACATATTTAAAACCATAGTTACTCAATTTTTTTTCTCTAAAAAATATCATCTAGATTTTCTCATGAATGGTCGATTACCATACAGGTCGGGATGCAACGAACACAACTTTGCAAGCAGAATGGAATGTTTTAGATGCAGTGCTCCGAGAGACTCGTACTAACGGAGCTAGTCAAGCCAAGATCCTCCGTCATCAAGTTTTGGGTGTGTGAAAATCAATTATTAAGAAAATAACATTTACAGTATAACTGTCAACCGTTTGCTTAACCTGTTTTCCTTTTTTTTTTAATCTAAAATTTTTTAAAGTCAAAATAATTTCTAATTATATATTCAATCATTGATATATTTTTATTTTTCTTCCATGTTTTTTGCAGGTAATGTTTCCAACTGAGCAAATTTCAAAGAGTCACTTCATAACCTGCATTAGCTTTCTTCTTCTTCTCTCTGTCTGTCTCTTTTTTCTTTTTTAGTTTTTGTTTTTTTTTTTGTAACTTTTTCTTTAATTTCTGGGTGCCTGTTATTCCTGTGCTGAAAATTTGAGATATTGTCCAAAATCAATGTTCATTATCAGCTGCTGTAATATCTCTTAATTAACTTCTTCTAAGCCTTATGAAATTAAAAAAAAATATATAAAAAATAAAAAGAAGGAAGAAGAAGAAGAAAGGTTTTTTTTTTCTTTTTTTTTTCCTTGGATTTCCCATGATAATTATCTAAACATCTAAAGTAGGAGAAATGTTTATTTGCTTTCTTCTATTCTGAAAAGGAGGGAGAAAAAAGGAAAAGAAAAAGAGAG

mRNA sequence

AAAAAGGTTTGGAAGCAAAGTATTGGCAATACTCCACTTTTTGAGTGGGATTTGGGTGTATATAAACGCCTCTTTCCCTTCTTTACCTATTCCATCGCCATTTCTTCATTCTTCATTCTTCATTCTTCACCATTACTGCACCACAGCAGCTCCTCTCCTTCACATTTCTCCTTCCGAGAGAAGATGAGCAGACCAGGAGACTGGAATTGCAGGTCGTGCCAGCACTTGAACTTCCAGAGGAGGGACACGTGCCAGCGCTGCGGCGACCCCAAGAGCGGCGGCGCTGATTTTGGCGGTCTAATGAACGGCGGCGGAAGAGGAGGCTCCGCATTCGGCTACGGCGGCTCCGACGTCAGGCCCGGAGACTGGTACTGTACCGTCGGCAATTGTGGAGCCCACAATTTCGCTAGCCGCTCGAGCTGCTTCAAATGCGGTGCCTTCAAAGACGACATGTTGGGTGGAGGCTTCGACTTCGATCTTCCTCGCTCCAGAGGAACCTCCTTCGGCTTTGCCAATGGCACCGGTGCTGGCGGCCGAACTGGTGCCTCTGCCTGGAAGTCTGGAGACTGGATTTGTGCCCGGTCGGGATGCAACGAACACAACTTTGCAAGCAGAATGGAATGTTTTAGATGCAGTGCTCCGAGAGACTCGTACTAACGGAGCTAGTCAAGCCAAGATCCTCCGTCATCAAGTTTTGGGTAATGTTTCCAACTGAGCAAATTTCAAAGAGTCACTTCATAACCTGCATTAGCTTTCTTCTTCTTCTCTCTGTCTGTCTCTTTTTTCTTTTTTAGTTTTTGTTTTTTTTTTTGTAACTTTTTCTTTAATTTCTGGGTGCCTGTTATTCCTGTGCTGAAAATTTGAGATATTGTCCAAAATCAATGTTCATTATCAGCTGCTGTAATATCTCTTAATTAACTTCTTCTAAGCCTTATGAAATTAAAAAAAAATATATAAAAAATAAAAAGAAGGAAGAAGAAGAAGAAAGGTTTTTTTTTTCTTTTTTTTTTCCTTGGATTTCCCATGATAATTATCTAAACATCTAAAGTAGGAGAAATGTTTATTTGCTTTCTTCTATTCTGAAAAGGAGGGAGAAAAAAGGAAAAGAAAAAGAGAG

Coding sequence (CDS)

AAAAAGGTTTGGAAGCAAAGTATTGGCAATACTCCACTTTTTGAGTGGGATTTGGGTGTATATAAACGCCTCTTTCCCTTCTTTACCTATTCCATCGCCATTTCTTCATTCTTCATTCTTCATTCTTCACCATTACTGCACCACAGCAGCTCCTCTCCTTCACATTTCTCCTTCCGAGAGAAGATGAGCAGACCAGGAGACTGGAATTGCAGGTCGTGCCAGCACTTGAACTTCCAGAGGAGGGACACGTGCCAGCGCTGCGGCGACCCCAAGAGCGGCGGCGCTGATTTTGGCGGTCTAATGAACGGCGGCGGAAGAGGAGGCTCCGCATTCGGCTACGGCGGCTCCGACGTCAGGCCCGGAGACTGGTACTGTACCGTCGGCAATTGTGGAGCCCACAATTTCGCTAGCCGCTCGAGCTGCTTCAAATGCGGTGCCTTCAAAGACGACATGTTGGGTGGAGGCTTCGACTTCGATCTTCCTCGCTCCAGAGGAACCTCCTTCGGCTTTGCCAATGGCACCGGTGCTGGCGGCCGAACTGGTGCCTCTGCCTGGAAGTCTGGAGACTGGATTTGTGCCCGGTCGGGATGCAACGAACACAACTTTGCAAGCAGAATGGAATGTTTTAGATGCAGTGCTCCGAGAGACTCGTACTAA

Protein sequence

KKVWKQSIGNTPLFEWDLGVYKRLFPFFTYSIAISSFFILHSSPLLHHSSSSPSHFSFREKMSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAGGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY
Homology
BLAST of MC08g1004 vs. ExPASy Swiss-Prot
Match: O13801 (Uncharacterized RNA-binding protein C17H9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC17H9.04c PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.5e-06
Identity = 52/181 (28.73%), Postives = 66/181 (36.46%), Query Frame = 0

Query: 42  SSPLLHHSSSSPSHFSFREKMSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLM 101
           S+ +L  +S     F   +   RPGDWNC  C   NFQRR +C RC  P   G       
Sbjct: 321 STKVLDKASDILIPFPSSKNRPRPGDWNCPMCGFSNFQRRTSCFRCSFP---GPTHVSAA 380

Query: 102 NGGGRGGSAFGYGGSDVRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLP 161
            G       F YG S           GN  +H  A+               GG       
Sbjct: 381 TGSNTFSPDFPYGNS----------YGNGSSHFIAN--------------YGGSVHHSNE 440

Query: 162 RSRGTSFGFANGTGAGGRTGAS-------AWKSGDWICARSGCNEHNFASRMECFRCSAP 216
            +  +     NG  A     +S        +++GDW C   GC  HNFA  + C RC A 
Sbjct: 441 NTMQSDLQHQNGNNAVNHHHSSRSFGGNVPFRAGDWKCGSEGCGYHNFAKNVCCLRCGAS 474

BLAST of MC08g1004 vs. ExPASy Swiss-Prot
Match: Q92804 (TATA-binding protein-associated factor 2N OS=Homo sapiens OX=9606 GN=TAF15 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.8e-05
Identity = 47/133 (35.34%), Postives = 59/133 (44.36%), Query Frame = 0

Query: 64  RPGDWNC--RSCQHLNFQRRDTCQRCGDP-----KSGGADFGGLMNG--------GGRGG 123
           + GDW C   SC ++NF RR++C +C +P     +  G DF G   G        GGRGG
Sbjct: 354 KSGDWVCPNPSCGNMNFARRNSCNQCNEPRPEDSRPSGGDFRGRGYGGERGYRGRGGRGG 413

Query: 124 SAFGYGGSDVRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSF 182
              GYGG   R G  Y    + G      RS     G +  D  GGG+  D    RG  +
Sbjct: 414 DRGGYGGD--RSGGGYGGDRSSGGGYSGDRSG----GGYGGDRSGGGYGGD----RGGGY 473

BLAST of MC08g1004 vs. ExPASy Swiss-Prot
Match: O95218 (Zinc finger Ran-binding domain-containing protein 2 OS=Homo sapiens OX=9606 GN=ZRANB2 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 2.5e-04
Identity = 31/98 (31.63%), Postives = 44/98 (44.90%), Query Frame = 0

Query: 118 VRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAG 177
           V  GDW C    CG  NFA R+SC +CG  K        +  + ++ GT  G      + 
Sbjct: 8   VSDGDWICPDKKCGNVNFARRTSCNRCGREKTT------EAKMMKAGGTEIGKTLAEKSR 67

Query: 178 GRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPR 216
           G      + + DW C    C+  N+A R EC  C+ P+
Sbjct: 68  G-----LFSANDWQC--KTCSNVNWARRSECNMCNTPK 92

BLAST of MC08g1004 vs. ExPASy Swiss-Prot
Match: Q9R020 (Zinc finger Ran-binding domain-containing protein 2 OS=Mus musculus OX=10090 GN=Zranb2 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 2.5e-04
Identity = 31/98 (31.63%), Postives = 44/98 (44.90%), Query Frame = 0

Query: 118 VRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAG 177
           V  GDW C    CG  NFA R+SC +CG  K        +  + ++ GT  G      + 
Sbjct: 8   VSDGDWICPDKKCGNVNFARRTSCNRCGREKTT------EAKMMKAGGTEIGKTLAEKSR 67

Query: 178 GRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPR 216
           G      + + DW C    C+  N+A R EC  C+ P+
Sbjct: 68  G-----LFSANDWQC--KTCSNVNWARRSECNMCNTPK 92

BLAST of MC08g1004 vs. ExPASy Swiss-Prot
Match: Q19QU3 (Zinc finger Ran-binding domain-containing protein 2 OS=Sus scrofa OX=9823 GN=ZRANB2 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.5e-04
Identity = 31/98 (31.63%), Postives = 44/98 (44.90%), Query Frame = 0

Query: 118 VRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAG 177
           V  GDW C    CG  NFA R+SC +CG  K        +  + ++ GT  G      + 
Sbjct: 8   VSDGDWICPDKKCGNVNFARRTSCNRCGREKTT------EAKMMKAGGTEIGKTLAEKSR 67

Query: 178 GRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPR 216
           G      + + DW C    C+  N+A R EC  C+ P+
Sbjct: 68  G-----LFSANDWQC--KTCSNVNWARRSECNMCNTPK 92

BLAST of MC08g1004 vs. NCBI nr
Match: XP_022153552.1 (TATA-binding protein-associated factor 2N-like [Momordica charantia])

HSP 1 Score: 341 bits (875), Expect = 1.24e-117
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPG 121
           MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPG
Sbjct: 1   MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPG 60

Query: 122 DWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAGGRTG 181
           DWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAGGRTG
Sbjct: 61  DWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAGGRTG 120

Query: 182 ASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
           ASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY
Sbjct: 121 ASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 157

BLAST of MC08g1004 vs. NCBI nr
Match: XP_022948551.1 (TATA-binding protein-associated factor 2N isoform X3 [Cucurbita moschata])

HSP 1 Score: 295 bits (756), Expect = 2.00e-99
Identity = 140/162 (86.42%), Postives = 145/162 (89.51%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGA--DFGGLMNGGGRGGSAFGYGGSDVR 121
           MSRPGDWNCRSCQHLNFQRRD CQRCGDPKSGG   DFG L+NGGGRGGS FGYGGSDVR
Sbjct: 1   MSRPGDWNCRSCQHLNFQRRDMCQRCGDPKSGGGGCDFGALLNGGGRGGSGFGYGGSDVR 60

Query: 122 PGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM--LGGGFDFDLPRSRGTS-FGFANGTGA 181
           PGDWYC++GNCGAHNFASRSSCFKCGAFKDDM    GGFDFD+PRSRG S FGFAN   A
Sbjct: 61  PGDWYCSIGNCGAHNFASRSSCFKCGAFKDDMSAAAGGFDFDMPRSRGLSPFGFANPAAA 120

Query: 182 GGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
             RTGASAWKSGDWICARSGCNEHNFASRMECFRCSAP+DSY
Sbjct: 121 AARTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPKDSY 162

BLAST of MC08g1004 vs. NCBI nr
Match: XP_022998918.1 (TATA-binding protein-associated factor 2N isoform X3 [Cucurbita maxima])

HSP 1 Score: 294 bits (752), Expect = 8.12e-99
Identity = 139/162 (85.80%), Postives = 144/162 (88.89%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGA--DFGGLMNGGGRGGSAFGYGGSDVR 121
           MSRPGDWNCRSCQHLNFQRRD CQRCGDPKSGG   DFG L+NGGGRGGS FGYGGSDVR
Sbjct: 1   MSRPGDWNCRSCQHLNFQRRDMCQRCGDPKSGGGGCDFGALLNGGGRGGSGFGYGGSDVR 60

Query: 122 PGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM--LGGGFDFDLPRSRGTS-FGFANGTGA 181
           PGDWYC++GNCGAHNFASRSSCFKCGAFKDDM    GGFDFD+PRSRG S FGFAN   A
Sbjct: 61  PGDWYCSIGNCGAHNFASRSSCFKCGAFKDDMSAAAGGFDFDMPRSRGLSPFGFANPAAA 120

Query: 182 GGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
             RTG SAWKSGDWICARSGCNEHNFASRMECFRCSAP+DSY
Sbjct: 121 AARTGTSAWKSGDWICARSGCNEHNFASRMECFRCSAPKDSY 162

BLAST of MC08g1004 vs. NCBI nr
Match: XP_022948549.1 (periodic tryptophan protein 2 isoform X2 [Cucurbita moschata] >XP_022948550.1 periodic tryptophan protein 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 299 bits (766), Expect = 2.61e-98
Identity = 142/164 (86.59%), Postives = 147/164 (89.63%), Query Frame = 0

Query: 60  EKMSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGA--DFGGLMNGGGRGGSAFGYGGSD 119
           EKMSRPGDWNCRSCQHLNFQRRD CQRCGDPKSGG   DFG L+NGGGRGGS FGYGGSD
Sbjct: 179 EKMSRPGDWNCRSCQHLNFQRRDMCQRCGDPKSGGGGCDFGALLNGGGRGGSGFGYGGSD 238

Query: 120 VRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM--LGGGFDFDLPRSRGTS-FGFANGT 179
           VRPGDWYC++GNCGAHNFASRSSCFKCGAFKDDM    GGFDFD+PRSRG S FGFAN  
Sbjct: 239 VRPGDWYCSIGNCGAHNFASRSSCFKCGAFKDDMSAAAGGFDFDMPRSRGLSPFGFANPA 298

Query: 180 GAGGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
            A  RTGASAWKSGDWICARSGCNEHNFASRMECFRCSAP+DSY
Sbjct: 299 AAAARTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPKDSY 342

BLAST of MC08g1004 vs. NCBI nr
Match: XP_023525213.1 (transcription initiation factor TFIID subunit 15b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291 bits (746), Expect = 6.89e-98
Identity = 140/163 (85.89%), Postives = 145/163 (88.96%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGA--DFGGLMNGGGRGGSAFGYGGSDVR 121
           MSRPGDWNCRSCQHLNFQRRD CQRCGDPKSGG   DFG L+NGGGRGGS FGYGGSDVR
Sbjct: 1   MSRPGDWNCRSCQHLNFQRRDMCQRCGDPKSGGGGCDFGALLNGGGRGGSGFGYGGSDVR 60

Query: 122 PGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM--LGGGFDFDLPRSRGTS-FGFAN-GTG 181
           PGDWYC++GNCGAHNFASRSSCFKCGAFKDDM    GGFDFD+PRSRG S FGFAN    
Sbjct: 61  PGDWYCSIGNCGAHNFASRSSCFKCGAFKDDMSAAAGGFDFDMPRSRGLSPFGFANPAAA 120

Query: 182 AGGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
           A  RTGASAWKSGDWICARSGCNEHNFASRMECFRCSAP+DSY
Sbjct: 121 AAARTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPKDSY 163

BLAST of MC08g1004 vs. ExPASy TrEMBL
Match: A0A6J1DJ84 (TATA-binding protein-associated factor 2N-like OS=Momordica charantia OX=3673 GN=LOC111021028 PE=4 SV=1)

HSP 1 Score: 341 bits (875), Expect = 5.99e-118
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPG 121
           MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPG
Sbjct: 1   MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPG 60

Query: 122 DWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAGGRTG 181
           DWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAGGRTG
Sbjct: 61  DWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAGGRTG 120

Query: 182 ASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
           ASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY
Sbjct: 121 ASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 157

BLAST of MC08g1004 vs. ExPASy TrEMBL
Match: A0A6J1G9I4 (TATA-binding protein-associated factor 2N isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111452195 PE=4 SV=1)

HSP 1 Score: 295 bits (756), Expect = 9.67e-100
Identity = 140/162 (86.42%), Postives = 145/162 (89.51%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGA--DFGGLMNGGGRGGSAFGYGGSDVR 121
           MSRPGDWNCRSCQHLNFQRRD CQRCGDPKSGG   DFG L+NGGGRGGS FGYGGSDVR
Sbjct: 1   MSRPGDWNCRSCQHLNFQRRDMCQRCGDPKSGGGGCDFGALLNGGGRGGSGFGYGGSDVR 60

Query: 122 PGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM--LGGGFDFDLPRSRGTS-FGFANGTGA 181
           PGDWYC++GNCGAHNFASRSSCFKCGAFKDDM    GGFDFD+PRSRG S FGFAN   A
Sbjct: 61  PGDWYCSIGNCGAHNFASRSSCFKCGAFKDDMSAAAGGFDFDMPRSRGLSPFGFANPAAA 120

Query: 182 GGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
             RTGASAWKSGDWICARSGCNEHNFASRMECFRCSAP+DSY
Sbjct: 121 AARTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPKDSY 162

BLAST of MC08g1004 vs. ExPASy TrEMBL
Match: A0A6J1KI51 (TATA-binding protein-associated factor 2N isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111493437 PE=4 SV=1)

HSP 1 Score: 294 bits (752), Expect = 3.93e-99
Identity = 139/162 (85.80%), Postives = 144/162 (88.89%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGA--DFGGLMNGGGRGGSAFGYGGSDVR 121
           MSRPGDWNCRSCQHLNFQRRD CQRCGDPKSGG   DFG L+NGGGRGGS FGYGGSDVR
Sbjct: 1   MSRPGDWNCRSCQHLNFQRRDMCQRCGDPKSGGGGCDFGALLNGGGRGGSGFGYGGSDVR 60

Query: 122 PGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM--LGGGFDFDLPRSRGTS-FGFANGTGA 181
           PGDWYC++GNCGAHNFASRSSCFKCGAFKDDM    GGFDFD+PRSRG S FGFAN   A
Sbjct: 61  PGDWYCSIGNCGAHNFASRSSCFKCGAFKDDMSAAAGGFDFDMPRSRGLSPFGFANPAAA 120

Query: 182 GGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
             RTG SAWKSGDWICARSGCNEHNFASRMECFRCSAP+DSY
Sbjct: 121 AARTGTSAWKSGDWICARSGCNEHNFASRMECFRCSAPKDSY 162

BLAST of MC08g1004 vs. ExPASy TrEMBL
Match: A0A6J1GA82 (periodic tryptophan protein 2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452195 PE=4 SV=1)

HSP 1 Score: 299 bits (766), Expect = 1.26e-98
Identity = 142/164 (86.59%), Postives = 147/164 (89.63%), Query Frame = 0

Query: 60  EKMSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGA--DFGGLMNGGGRGGSAFGYGGSD 119
           EKMSRPGDWNCRSCQHLNFQRRD CQRCGDPKSGG   DFG L+NGGGRGGS FGYGGSD
Sbjct: 179 EKMSRPGDWNCRSCQHLNFQRRDMCQRCGDPKSGGGGCDFGALLNGGGRGGSGFGYGGSD 238

Query: 120 VRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM--LGGGFDFDLPRSRGTS-FGFANGT 179
           VRPGDWYC++GNCGAHNFASRSSCFKCGAFKDDM    GGFDFD+PRSRG S FGFAN  
Sbjct: 239 VRPGDWYCSIGNCGAHNFASRSSCFKCGAFKDDMSAAAGGFDFDMPRSRGLSPFGFANPA 298

Query: 180 GAGGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
            A  RTGASAWKSGDWICARSGCNEHNFASRMECFRCSAP+DSY
Sbjct: 299 AAAARTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPKDSY 342

BLAST of MC08g1004 vs. ExPASy TrEMBL
Match: A0A6J1K9C2 (periodic tryptophan protein 2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493437 PE=4 SV=1)

HSP 1 Score: 298 bits (762), Expect = 5.11e-98
Identity = 141/164 (85.98%), Postives = 146/164 (89.02%), Query Frame = 0

Query: 60  EKMSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGA--DFGGLMNGGGRGGSAFGYGGSD 119
           EKMSRPGDWNCRSCQHLNFQRRD CQRCGDPKSGG   DFG L+NGGGRGGS FGYGGSD
Sbjct: 179 EKMSRPGDWNCRSCQHLNFQRRDMCQRCGDPKSGGGGCDFGALLNGGGRGGSGFGYGGSD 238

Query: 120 VRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM--LGGGFDFDLPRSRGTS-FGFANGT 179
           VRPGDWYC++GNCGAHNFASRSSCFKCGAFKDDM    GGFDFD+PRSRG S FGFAN  
Sbjct: 239 VRPGDWYCSIGNCGAHNFASRSSCFKCGAFKDDMSAAAGGFDFDMPRSRGLSPFGFANPA 298

Query: 180 GAGGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRDSY 218
            A  RTG SAWKSGDWICARSGCNEHNFASRMECFRCSAP+DSY
Sbjct: 299 AAAARTGTSAWKSGDWICARSGCNEHNFASRMECFRCSAPKDSY 342

BLAST of MC08g1004 vs. TAIR 10
Match: AT3G15680.1 (Ran BP2/NZF zinc finger-like superfamily protein )

HSP 1 Score: 216.1 bits (549), Expect = 2.8e-56
Identity = 111/168 (66.07%), Postives = 119/168 (70.83%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGL--MNGGGRGGSAFGY-GGSDV 121
           MSRPGDWNCRSC HLNFQRRD+CQRCGD +SG    GGL   N GGR  SAFG+  GSDV
Sbjct: 1   MSRPGDWNCRSCSHLNFQRRDSCQRCGDSRSGPGGVGGLDFGNFGGRAMSAFGFTTGSDV 60

Query: 122 RPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGGG----------FDFDLPRSRGTSF 181
           RPGDWYCTVGNCG HNFASRS+CFKCG FKD+   GG          FD D+ RSR    
Sbjct: 61  RPGDWYCTVGNCGTHNFASRSTCFKCGTFKDETGAGGGGGGIGGPAMFDADIMRSR---- 120

Query: 182 GFANGTGAGGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRD 217
                    G  G S+WKSGDWIC R GCNEHNFASRMECFRC+APRD
Sbjct: 121 -------VPGNGGRSSWKSGDWICTRIGCNEHNFASRMECFRCNAPRD 157

BLAST of MC08g1004 vs. TAIR 10
Match: AT5G25490.1 (Ran BP2/NZF zinc finger-like superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-50
Identity = 99/164 (60.37%), Postives = 115/164 (70.12%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRG-GSAFGYG-GSDVR 121
           M+RPGDWNCR C HLNFQRRD+CQRC +P+ GG     L   GGR   S+FG+  G DVR
Sbjct: 1   MNRPGDWNCRLCSHLNFQRRDSCQRCREPRPGGISTDLLSGFGGRPVSSSFGFNTGPDVR 60

Query: 122 PGDWYCTVGNCGAHNFASRSSCFKCGAFKDDM-------LGGGFDFDLPRSRGTSFGFAN 181
           PGDWYC +G+CG HNFA+RSSCFKCGA KD+          G  D ++   RG  FGF  
Sbjct: 61  PGDWYCNLGDCGTHNFANRSSCFKCGAAKDEFSCSSAAATTGFMDMNVGPRRGL-FGFGG 120

Query: 182 GTGAGGRTGASAWKSGDWICARSGCNEHNFASRMECFRCSAPRD 217
            +  GG TG S WKSGDWIC RSGCNEHNFASR ECFRC+AP++
Sbjct: 121 SSSGGGGTGRSPWKSGDWICPRSGCNEHNFASRSECFRCNAPKE 163

BLAST of MC08g1004 vs. TAIR 10
Match: AT2G26695.1 (Ran BP2/NZF zinc finger-like superfamily protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.5e-30
Identity = 65/154 (42.21%), Postives = 86/154 (55.84%), Query Frame = 0

Query: 66  GDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPGDWYC 125
           GDW C +CQH NF++R++CQ+CG PK GG D            S + Y  ++V  GDWYC
Sbjct: 6   GDWLCGACQHANFKKRESCQKCGYPKFGGVDV-----------STYLYNRTEVMAGDWYC 65

Query: 126 TVGNCGAHNFASRSSCFKCGAFKDDMLGGGFDFDLPRSRGTSFGFANGTGAGGRTGAS-- 185
              NCG+HN+ASR+SC++CG  K +               T   +     A G  GA+  
Sbjct: 66  GALNCGSHNYASRTSCYRCGMIKVEY--------------TEQYYGAQMVAYGNDGAACP 125

Query: 186 -AWKSGDWICARSGCNEHNFASRMECFRCSAPRD 217
             WK+GDW+C R GC  HN+ASR ECF+C   RD
Sbjct: 126 PGWKTGDWVCPRVGCGVHNYASRAECFKCKTTRD 134

BLAST of MC08g1004 vs. TAIR 10
Match: AT2G17975.1 (zinc finger (Ran-binding) family protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-09
Identity = 59/216 (27.31%), Postives = 78/216 (36.11%), Query Frame = 0

Query: 62  MSRPGDWNCRSCQHLNFQRRDTCQRCGDPKS---------GGADFGGLMNGGGRGGSAFG 121
           + R GDW C  C + N+  R+ C++CG  K           GA     ++   RG  +  
Sbjct: 46  LPRIGDWICTGCTNNNYASREKCKKCGQSKEVAALSALAIPGASLQTHLHYFTRGPESHD 105

Query: 122 YGGS---------------DVRPGDWYCTVGNCGAHNFASRSSCFKCGAFKDDMLGG--- 181
             GS               + R GDW C    CG HN++SR  C KC       LG    
Sbjct: 106 QPGSLLAFSNATNQASVHKEWRSGDWIC---RCGFHNYSSRIQCKKCNEIAPLALGTKRL 165

Query: 182 -----GFDFDLPRSR-----------------GTSFGFA------------NGTGAGGRT 217
                  ++D  R                   G S G              + T A    
Sbjct: 166 ASEALAHEWDSKRLNQGYTSMQTQSAIYASFPGMSLGRVSNWQLPLPFLQQHSTPALLGM 225

BLAST of MC08g1004 vs. TAIR 10
Match: AT1G70650.1 (Ran BP2/NZF zinc finger-like superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 3.3e-04
Identity = 23/81 (28.40%), Postives = 31/81 (38.27%), Query Frame = 0

Query: 64  RPGDWNCRSCQHLNFQRRDTCQRCGDPKSGGADFGGLMNGGGRGGSAFGYGGSDVRPGDW 123
           + GDW C++C  LNF +   C RC D  +                         + PG+W
Sbjct: 279 KKGDWICQTCNFLNFSKNTRCLRCKDKPT----------------------LRQINPGEW 335

Query: 124 YCTVGNCGAHNFASRSSCFKC 145
            C   +C   NF   S C KC
Sbjct: 339 ECE--SCNYINFRRNSICLKC 335

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O138011.5e-0628.73Uncharacterized RNA-binding protein C17H9.04c OS=Schizosaccharomyces pombe (stra... [more]
Q928043.8e-0535.34TATA-binding protein-associated factor 2N OS=Homo sapiens OX=9606 GN=TAF15 PE=1 ... [more]
O952182.5e-0431.63Zinc finger Ran-binding domain-containing protein 2 OS=Homo sapiens OX=9606 GN=Z... [more]
Q9R0202.5e-0431.63Zinc finger Ran-binding domain-containing protein 2 OS=Mus musculus OX=10090 GN=... [more]
Q19QU32.5e-0431.63Zinc finger Ran-binding domain-containing protein 2 OS=Sus scrofa OX=9823 GN=ZRA... [more]
Match NameE-valueIdentityDescription
XP_022153552.11.24e-117100.00TATA-binding protein-associated factor 2N-like [Momordica charantia][more]
XP_022948551.12.00e-9986.42TATA-binding protein-associated factor 2N isoform X3 [Cucurbita moschata][more]
XP_022998918.18.12e-9985.80TATA-binding protein-associated factor 2N isoform X3 [Cucurbita maxima][more]
XP_022948549.12.61e-9886.59periodic tryptophan protein 2 isoform X2 [Cucurbita moschata] >XP_022948550.1 pe... [more]
XP_023525213.16.89e-9885.89transcription initiation factor TFIID subunit 15b-like [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
A0A6J1DJ845.99e-118100.00TATA-binding protein-associated factor 2N-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1G9I49.67e-10086.42TATA-binding protein-associated factor 2N isoform X3 OS=Cucurbita moschata OX=36... [more]
A0A6J1KI513.93e-9985.80TATA-binding protein-associated factor 2N isoform X3 OS=Cucurbita maxima OX=3661... [more]
A0A6J1GA821.26e-9886.59periodic tryptophan protein 2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1K9C25.11e-9885.98periodic tryptophan protein 2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
Match NameE-valueIdentityDescription
AT3G15680.12.8e-5666.07Ran BP2/NZF zinc finger-like superfamily protein [more]
AT5G25490.11.0e-5060.37Ran BP2/NZF zinc finger-like superfamily protein [more]
AT2G26695.13.5e-3042.21Ran BP2/NZF zinc finger-like superfamily protein [more]
AT2G17975.12.0e-0927.31zinc finger (Ran-binding) family protein [more]
AT1G70650.13.3e-0428.40Ran BP2/NZF zinc finger-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001876Zinc finger, RanBP2-typeSMARTSM00547zf_4coord: 121..147
e-value: 2.2E-5
score: 33.9
coord: 66..90
e-value: 8.4E-6
score: 35.3
coord: 188..214
e-value: 2.1E-5
score: 34.0
IPR001876Zinc finger, RanBP2-typePFAMPF00641zf-RanBPcoord: 119..146
e-value: 2.2E-5
score: 23.7
coord: 186..215
e-value: 2.1E-10
score: 39.7
coord: 64..92
e-value: 3.9E-4
score: 19.7
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 123..144
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 190..211
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 68..87
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 119..150
score: 9.890478
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 64..93
score: 9.788849
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 186..217
score: 10.545425
NoneNo IPR availableGENE3D4.10.1060.10coord: 180..217
e-value: 1.0E-12
score: 49.0
coord: 59..94
e-value: 1.4E-10
score: 42.2
coord: 115..150
e-value: 4.6E-12
score: 47.0
NoneNo IPR availablePANTHERPTHR23111ZINC FINGER PROTEINcoord: 40..217
NoneNo IPR availablePANTHERPTHR23111:SF74OS02G0203700 PROTEINcoord: 40..217
IPR036443Zinc finger, RanBP2-type superfamilySUPERFAMILY90209Ran binding protein zinc finger-likecoord: 181..216
IPR036443Zinc finger, RanBP2-type superfamilySUPERFAMILY90209Ran binding protein zinc finger-likecoord: 60..94
IPR036443Zinc finger, RanBP2-type superfamilySUPERFAMILY90209Ran binding protein zinc finger-likecoord: 112..149

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1004.1MC08g1004.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003729 mRNA binding