MC08g1001 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g1001
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	MAPK kinase substrate protein At1g80180-like
Location	MC08: 8113014 .. 8113382 (-)
RNA-Seq Expression	MC08g1001
Synteny	MC08g1001

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGTTTGCAGAGATCTGCGGTTTCTTTCAGAAGGCAAGGCTCATCGGGGCTGGTTTGGGACGACCGATTTCTGTCCGGCGAGTTGAAGAAAGCCGCCGCCACCACGTCGGACAAAGAATCGGGCCGGAACGAAGACGAAAATCCGGAGATTAAAGAGGTCAAATCGACGCCGGCGGCGGTGGAGCCGATCAATACTACCATCGAGAGGAGCCGATCCAACGGCGGCCGTGGGTACCGGACCGGAAAAGTGTCTCCGGCGATCGAACCGCCGTCGCCGAAGGTCTCTGCCTGTGGATTCTGCAGCGCGTTCGGGAAGCCGGCGAAGAAGAGTCGCCGGACGAAAACCGGTAAGCGTAGATCGCGG

mRNA sequence

ATGGAGGGTTTGCAGAGATCTGCGGTTTCTTTCAGAAGGCAAGGCTCATCGGGGCTGGTTTGGGACGACCGATTTCTGTCCGGCGAGTTGAAGAAAGCCGCCGCCACCACGTCGGACAAAGAATCGGGCCGGAACGAAGACGAAAATCCGGAGATTAAAGAGGTCAAATCGACGCCGGCGGCGGTGGAGCCGATCAATACTACCATCGAGAGGAGCCGATCCAACGGCGGCCGTGGGTACCGGACCGGAAAAGTGTCTCCGGCGATCGAACCGCCGTCGCCGAAGGTCTCTGCCTGTGGATTCTGCAGCGCGTTCGGGAAGCCGGCGAAGAAGAGTCGCCGGACGAAAACCGGTAAGCGTAGATCGCGG

Coding sequence (CDS)

ATGGAGGGTTTGCAGAGATCTGCGGTTTCTTTCAGAAGGCAAGGCTCATCGGGGCTGGTTTGGGACGACCGATTTCTGTCCGGCGAGTTGAAGAAAGCCGCCGCCACCACGTCGGACAAAGAATCGGGCCGGAACGAAGACGAAAATCCGGAGATTAAAGAGGTCAAATCGACGCCGGCGGCGGTGGAGCCGATCAATACTACCATCGAGAGGAGCCGATCCAACGGCGGCCGTGGGTACCGGACCGGAAAAGTGTCTCCGGCGATCGAACCGCCGTCGCCGAAGGTCTCTGCCTGTGGATTCTGCAGCGCGTTCGGGAAGCCGGCGAAGAAGAGTCGCCGGACGAAAACCGGTAAGCGTAGATCGCGG

Protein sequence

MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPAAVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKRRSR

Homology

[Close]

BLAST of MC08g1001 vs. ExPASy Swiss-Prot
Match: Q9XI29 (Uncharacterized protein At1g15400 OS=Arabidopsis thaliana OX=3702 GN=At1g15400 PE=1 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 4.7e-21
Identity = 70/142 (49.30%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENP------EIKE 60
           MEGLQRS +SFRRQGSSG+V+DDR ++ EL K+       ES R+E   P      ++K 
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  VKSTPAAVEPINT------TIERSRSNGGRGYR----TGKVSPAIEPPSPKVSACGFCSA 120
           +      + PI T       IERSRSNGG   R    TG+VSPA++PPSP++S+CG CSA
Sbjct: 61  IDEKD-KLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSA 120

Query: 121 FGK--PAKK-SRRTKTGKRRSR 124
           FGK  P KK + R +  KRRSR
Sbjct: 121 FGKNPPGKKVNPRKRPPKRRSR 140

[Close]

BLAST of MC08g1001 vs. ExPASy Swiss-Prot
Match: Q9SSC1 (MAPK kinase substrate protein At1g80180 OS=Arabidopsis thaliana OX=3702 GN=At1g80180 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 8.1e-21
Identity = 68/139 (48.92%), Postives = 93/139 (66.91%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDE----NPEIKEVK 60
           M GLQRS +SFRRQGSSG+VWDDR ++ EL + AA     E+ + +++      E+++  
Sbjct: 1   MAGLQRSTISFRRQGSSGIVWDDRLIA-ELSQQAANDRKGETLQQDEQAKLITSEVQDQT 60

Query: 61  STPAA---VEPINTT--IERSRSNGGRGYR----TGKVSPAIEPPSPKVSACGFCSAFGK 120
           + P A   ++PI T   +ERSRSNGG   R    TG+VSPA++PPSP++SA G CSAFGK
Sbjct: 61  TKPIAGEGLKPIRTDGGMERSRSNGGGAIRHHRNTGRVSPAVDPPSPRLSAFGCCSAFGK 120

Query: 121 --PAKK-SRRTKTGKRRSR 124
             P KK ++R +  KRRSR
Sbjct: 121 KQPGKKVNQRKRPAKRRSR 138

[Close]

BLAST of MC08g1001 vs. NCBI nr
Match: KAG6606789.1 (MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036501.1 MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 196 bits (499), Expect = 1.55e-62
Identity = 105/124 (84.68%), Postives = 111/124 (89.52%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ AA  ++KES    +ENP+ K    TPA
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTAAAAAEKES----EENPDAKST--TPA 60

Query: 61  -AVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK 120
            A+EPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK
Sbjct: 61  VALEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK 118

Query: 121 RRSR 123
           RRSR
Sbjct: 121 RRSR 118

[Close]

BLAST of MC08g1001 vs. NCBI nr
Match: XP_022948995.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita moschata])

HSP 1 Score: 195 bits (495), Expect = 6.30e-62
Identity = 105/123 (85.37%), Postives = 109/123 (88.62%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ AA  ++KES     ENPE K    TPA
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTAAAAAEKESV----ENPEAKST--TPA 60

Query: 61  -AVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK 120
            A+EPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK
Sbjct: 61  VALEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK 117

Query: 121 RRS 122
           RRS
Sbjct: 121 RRS 117

[Close]

BLAST of MC08g1001 vs. NCBI nr
Match: XP_011654316.1 (MAPK kinase substrate protein At1g80180 [Cucumis sativus] >KGN52876.1 hypothetical protein Csa_015127 [Cucumis sativus])

HSP 1 Score: 192 bits (487), Expect = 1.11e-60
Identity = 102/123 (82.93%), Postives = 108/123 (87.80%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ A TTS+KES  N+D+        +  A
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTA-TTSEKESEENQDQGKST--AAAAAA 60

Query: 61  AVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 120
           AVEPI+ TIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR
Sbjct: 61  AVEPISITIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 120

Query: 121 RSR 123
           RSR
Sbjct: 121 RSR 120

[Close]

BLAST of MC08g1001 vs. NCBI nr
Match: XP_038899093.1 (MAPK kinase substrate protein At1g80180-like [Benincasa hispida])

HSP 1 Score: 191 bits (486), Expect = 1.35e-60
Identity = 103/123 (83.74%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ AA  S+KES  N+       E KST A
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTAAA-SEKESEENQ-------EAKSTTA 60

Query: 61  AVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 120
           AVEPI+ TIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKP KKSRRTKTGKR
Sbjct: 61  AVEPISITIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPTKKSRRTKTGKR 115

Query: 121 RSR 123
           RSR
Sbjct: 121 RSR 115

[Close]

BLAST of MC08g1001 vs. NCBI nr
Match: XP_022998926.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita maxima])

HSP 1 Score: 191 bits (486), Expect = 1.44e-60
Identity = 105/124 (84.68%), Postives = 110/124 (88.71%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ AA  S+KES    +ENPE K    TPA
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTAAA-SEKES----EENPEAKST--TPA 60

Query: 61  A-VEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK 120
             +EPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKK+RRTKTGK
Sbjct: 61  VTLEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKARRTKTGK 117

Query: 121 RRSR 123
           RRSR
Sbjct: 121 RRSR 117

[Close]

BLAST of MC08g1001 vs. ExPASy TrEMBL
Match: A0A6J1GBH4 (MAPK kinase substrate protein At1g80180-like OS=Cucurbita moschata OX=3662 GN=LOC111452467 PE=4 SV=1)

HSP 1 Score: 195 bits (495), Expect = 3.05e-62
Identity = 105/123 (85.37%), Postives = 109/123 (88.62%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ AA  ++KES     ENPE K    TPA
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTAAAAAEKESV----ENPEAKST--TPA 60

Query: 61  -AVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK 120
            A+EPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK
Sbjct: 61  VALEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK 117

Query: 121 RRS 122
           RRS
Sbjct: 121 RRS 117

[Close]

BLAST of MC08g1001 vs. ExPASy TrEMBL
Match: A0A0A0KYE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G004870 PE=4 SV=1)

HSP 1 Score: 192 bits (487), Expect = 5.39e-61
Identity = 102/123 (82.93%), Postives = 108/123 (87.80%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ A TTS+KES  N+D+        +  A
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTA-TTSEKESEENQDQGKST--AAAAAA 60

Query: 61  AVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 120
           AVEPI+ TIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR
Sbjct: 61  AVEPISITIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 120

Query: 121 RSR 123
           RSR
Sbjct: 121 RSR 120

[Close]

BLAST of MC08g1001 vs. ExPASy TrEMBL
Match: A0A6J1KBK3 (MAPK kinase substrate protein At1g80180-like OS=Cucurbita maxima OX=3661 GN=LOC111493442 PE=4 SV=1)

HSP 1 Score: 191 bits (486), Expect = 6.96e-61
Identity = 105/124 (84.68%), Postives = 110/124 (88.71%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ AA  S+KES    +ENPE K    TPA
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTAAA-SEKES----EENPEAKST--TPA 60

Query: 61  A-VEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGK 120
             +EPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKK+RRTKTGK
Sbjct: 61  VTLEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKARRTKTGK 117

Query: 121 RRSR 123
           RRSR
Sbjct: 121 RRSR 117

[Close]

BLAST of MC08g1001 vs. ExPASy TrEMBL
Match: A0A5A7TMJ8 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold52012G00010 PE=4 SV=1)

HSP 1 Score: 191 bits (484), Expect = 1.45e-60
Identity = 101/123 (82.11%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ A TTS+KES  N+D+        +T  
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTA-TTSEKESEENQDQGKST----ATAT 60

Query: 61  AVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 120
            VEPI+ TIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR
Sbjct: 61  GVEPISITIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 118

Query: 121 RSR 123
           RSR
Sbjct: 121 RSR 118

[Close]

BLAST of MC08g1001 vs. ExPASy TrEMBL
Match: A0A1S4DZT3 (uncharacterized protein At1g15400-like OS=Cucumis melo OX=3656 GN=LOC107991268 PE=4 SV=1)

HSP 1 Score: 191 bits (484), Expect = 1.45e-60
Identity = 101/123 (82.11%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPA 60
           MEGLQRSAVSFRRQGSSGLVWDDRFLSGELK+ A TTS+KES  N+D+        +T  
Sbjct: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKQTA-TTSEKESEENQDQGKST----ATAT 60

Query: 61  AVEPINTTIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 120
            VEPI+ TIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR
Sbjct: 61  GVEPISITIERSRSNGGRGYRTGKVSPAIEPPSPKVSACGFCSAFGKPAKKSRRTKTGKR 118

Query: 121 RSR 123
           RSR
Sbjct: 121 RSR 118

[Close]

BLAST of MC08g1001 vs. TAIR 10
Match: AT1G15400.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 16 species: Archae - 0; Bacteria - 4; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 102.1 bits (253), Expect = 3.4e-22
Identity = 70/142 (49.30%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENP------EIKE 60
           MEGLQRS +SFRRQGSSG+V+DDR ++ EL K+       ES R+E   P      ++K 
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  VKSTPAAVEPINT------TIERSRSNGGRGYR----TGKVSPAIEPPSPKVSACGFCSA 120
           +      + PI T       IERSRSNGG   R    TG+VSPA++PPSP++S+CG CSA
Sbjct: 61  IDEKD-KLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSA 120

Query: 121 FGK--PAKK-SRRTKTGKRRSR 124
           FGK  P KK + R +  KRRSR
Sbjct: 121 FGKNPPGKKVNPRKRPPKRRSR 140

[Close]

BLAST of MC08g1001 vs. TAIR 10
Match: AT1G15400.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 16 species: Archae - 0; Bacteria - 4; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 102.1 bits (253), Expect = 3.4e-22
Identity = 70/142 (49.30%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENP------EIKE 60
           MEGLQRS +SFRRQGSSG+V+DDR ++ EL K+       ES R+E   P      ++K 
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  VKSTPAAVEPINT------TIERSRSNGGRGYR----TGKVSPAIEPPSPKVSACGFCSA 120
           +      + PI T       IERSRSNGG   R    TG+VSPA++PPSP++S+CG CSA
Sbjct: 61  IDEKD-KLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSA 120

Query: 121 FGK--PAKK-SRRTKTGKRRSR 124
           FGK  P KK + R +  KRRSR
Sbjct: 121 FGKNPPGKKVNPRKRPPKRRSR 140

[Close]

BLAST of MC08g1001 vs. TAIR 10
Match: AT1G15400.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 84 Blast hits to 84 proteins in 17 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 102.1 bits (253), Expect = 3.4e-22
Identity = 70/142 (49.30%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENP------EIKE 60
           MEGLQRS +SFRRQGSSG+V+DDR ++ EL K+       ES R+E   P      ++K 
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  VKSTPAAVEPINT------TIERSRSNGGRGYR----TGKVSPAIEPPSPKVSACGFCSA 120
           +      + PI T       IERSRSNGG   R    TG+VSPA++PPSP++S+CG CSA
Sbjct: 61  IDEKD-KLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSA 120

Query: 121 FGK--PAKK-SRRTKTGKRRSR 124
           FGK  P KK + R +  KRRSR
Sbjct: 121 FGKNPPGKKVNPRKRPPKRRSR 140

[Close]

BLAST of MC08g1001 vs. TAIR 10
Match: AT1G80180.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15400.3); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 101.3 bits (251), Expect = 5.7e-22
Identity = 68/139 (48.92%), Postives = 93/139 (66.91%), Query Frame = 0


Query: 1   MEGLQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDE----NPEIKEVK 60
           M GLQRS +SFRRQGSSG+VWDDR ++ EL + AA     E+ + +++      E+++  
Sbjct: 1   MAGLQRSTISFRRQGSSGIVWDDRLIA-ELSQQAANDRKGETLQQDEQAKLITSEVQDQT 60

Query: 61  STPAA---VEPINTT--IERSRSNGGRGYR----TGKVSPAIEPPSPKVSACGFCSAFGK 120
           + P A   ++PI T   +ERSRSNGG   R    TG+VSPA++PPSP++SA G CSAFGK
Sbjct: 61  TKPIAGEGLKPIRTDGGMERSRSNGGGAIRHHRNTGRVSPAVDPPSPRLSAFGCCSAFGK 120

Query: 121 --PAKK-SRRTKTGKRRSR 124
             P KK ++R +  KRRSR
Sbjct: 121 KQPGKKVNQRKRPAKRRSR 138

[Close]

BLAST of MC08g1001 vs. TAIR 10
Match: AT5G20100.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 82; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 89.7 bits (221), Expect = 1.7e-18
Identity = 56/123 (45.53%), Postives = 74/123 (60.16%), Query Frame = 0


Query: 4   LQRSAVSFRRQGSSGLVWDDRFLSGELKKAAATTSDKESGRNEDENPEIKEVKSTPAAVE 63
           LQRS  SFRRQGSSGL+W+DRFLSGE++               DE  E +       ++ 
Sbjct: 3   LQRSTASFRRQGSSGLIWNDRFLSGEIR--------------NDERQEDRCNDHRDGSMA 62

Query: 64  PINTTIERSRSNGGRGY--RTGKVSPAIEPPSPKVSA--CGFCSAFGKPAKKSRRTKTGK 123
               T++RS S+GG G+  R G++SPA++PPSPK+SA  CGFCS F    K+  R   G+
Sbjct: 63  ATTATVKRSASDGGCGHGGRLGELSPALDPPSPKISAVGCGFCSLFSSNGKRRSR---GR 108

The following BLAST results are available for this feature:

BLAST of MC08g1001 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XI29	4.7e-21	49.30	Uncharacterized protein At1g15400 OS=Arabidopsis thaliana OX=3702 GN=At1g15400 P...	[more]
Q9SSC1	8.1e-21	48.92	MAPK kinase substrate protein At1g80180 OS=Arabidopsis thaliana OX=3702 GN=At1g8...	[more]

BLAST of MC08g1001 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606789.1	1.55e-62	84.68	MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. sororia] >...	[more]
XP_022948995.1	6.30e-62	85.37	MAPK kinase substrate protein At1g80180-like [Cucurbita moschata]	[more]
XP_011654316.1	1.11e-60	82.93	MAPK kinase substrate protein At1g80180 [Cucumis sativus] >KGN52876.1 hypothetic...	[more]
XP_038899093.1	1.35e-60	83.74	MAPK kinase substrate protein At1g80180-like [Benincasa hispida]	[more]
XP_022998926.1	1.44e-60	84.68	MAPK kinase substrate protein At1g80180-like [Cucurbita maxima]	[more]

BLAST of MC08g1001 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBH4	3.05e-62	85.37	MAPK kinase substrate protein At1g80180-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0KYE3	5.39e-61	82.93	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G004870 PE=4 SV=1	[more]
A0A6J1KBK3	6.96e-61	84.68	MAPK kinase substrate protein At1g80180-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A5A7TMJ8	1.45e-60	82.11	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S4DZT3	1.45e-60	82.11	uncharacterized protein At1g15400-like OS=Cucumis melo OX=3656 GN=LOC107991268 P...	[more]

BLAST of MC08g1001 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15400.1	3.4e-22	49.30	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G15400.2	3.4e-22	49.30	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G15400.3	3.4e-22	49.30	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G80180.1	5.7e-22	48.92	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT5G20100.1	1.7e-18	45.53	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031421	Protein of unknown function DUF4666	PFAM	PF15697	DUF4666	coord: 1..112 e-value: 1.3E-37 score: 128.9
IPR031421	Protein of unknown function DUF4666	PANTHER	PTHR33730	OS05G0542732 PROTEIN-RELATED	coord: 1..120
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..54
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..93
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..75
None	No IPR available	PANTHER	PTHR33730:SF4	OS05G0542732 PROTEIN	coord: 1..120

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g1001.1	MC08g1001.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity