MC08g0884 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0884
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPectinesterase
LocationMC08: 7149800 .. 7153104 (-)
RNA-Seq ExpressionMC08g0884
SyntenyMC08g0884
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGTTATTAAAACTTTTCAATCAACCTCCATTTAAAATAGTATCATTTTCTTAGTGATCCAAACGGTCCGACCTTCTAATAAATAATATATGCTTCAAATCAATCCAGTTATATTTATGTCAGACATTTTAAACCTAGAGAAATTTTTTTAGTTTTTTTAGTATAACAAATAAGATGGGAAAATTCGAACTCATGTACCAATTATCGTTAAGATAAGCTAATGCTGACTATGACTGGTAGCACGACGATAAAATAAAGAAGAAACCAAGAAAACGAAAAAAAAAAATGGGGTCAGCAATGTGAATTTGACTGGAACACATGATTAGGAAGATTTATTCAAAATGTTTAAAAAAAAATAGTACGATGGATTACACATACGACATGCCATTTGCTTGTCCAACACTACATTACACATCTGACCTAATACGCGTAACCAATTCATACAAATAACTTAATTAAATTAATTAATCTAATTAATTTTGCTTTTCCCTCTTCTCTTCCCTTCGCGTTTGAAAGCCCCCCAATATGTTCGATTCCTCCACTCGCATTTCTCCATCGCCACGCATTTTCCTTTCCAAATCCCCAAACCTTCTCTCTCTGCAACTCTCAAATCCATGGATTCCGTCCAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAGCATCAAGCCTTCCGTCGGAAAACGCGCCGCCGACTCATCATTCTTCTTCTCTCCATCGTTCTCCTAATCGCCGTTGTCATCGGTGCCGTCGCCGGCATTCTAATCCGTAACCGGAACTCCTCCTCCTCCCCGCCCACCACCGCCGGTCCTCCCACAGAGCTCACTCCCGTCGCCTCGCTCAAAAGTCTCTGCAGCGTCACTCAGTATCCCAGCTCCTGCCTCTCCTCTCTCGCAAACTCCAACACCACCGATCCCGAGATTCTGTTCAAGCTTTCTCTCCGCGTCGCCTCCGATGCGCTCTCCAAACTCGCCGATTACTCCTCCACCCTCGCTTCCACTACCGATGACCCGAAAATCAGAGACGCAATCGGAATCTGCCGGAGCGTTTTCGAAGACGCCATTGACACTCTGAACGACACCGTTTCGTCCATGGAAGTGGATCGCCGTGGCCAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAGAACCTGGCTCAGCACCGCCATCACCGACCAGGAGACCTGCCTCGACGCCGTCCGAGATCTCAACCGGACGGCGGTTCTAGAAGATTTACAAGCAGCAATGGGGAACTCTACCGAATTCGCCAGCAATAGCTTAGCGATTGTGACTAAAATTCTGGCCGTGTTGGCCAATTTCAACATCCCAATCCACCGGAAGCTGATGGGGTTCCCGGAGTGGGTGAGCCCCGGCGACCGGAGGCTTCTGGAGGAGAACAATGTGACGACGCACGTGACGGTGTCGAAAGATGGGAAAGGGGACTTCTCGACGATCAAAGAGGCCGTAGCGGCGGTGCCAAAGAAGAGCAAAGAAAGATTCGTCATATATGTAAAAGAGGGAGTATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTATGGAGATGGCAGAACCAAAACCATTGTTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGGTAACCTATTTAATCACCCTTTTTCTCAATTGCTTCATTTCATTTACTTTTGCATAATGCATCCAGTCCAATCCAATCCATGTCTGCCATGTTCTTATTCTCGTTTAAACTTTATACCCTTTTTCTTGTTCTTGGCCTTTTTTAAGCCTCTGCCCCCAAATCGAAAACCCTTAAAATTTAAGGACAATTAGTGTAGTGTAGACTATAGTGAGGGGATGATGAGCCATTGCCCCTTATTTTCAGAAACCTCACATGGCCTACATGTCAGCCATGTGAACCCAATTTCCCCCACTTTCCCTTTTGGCCGGCCATTGATCTTTCCATGTGAATTGGCTTGTGTCTGTTCCCATCACATCATTACTAGACATCTAACCTCTTTCAAACCTAAAAGTTTAAACCAATGAATTAAATAATTTTAATTTATGTATTAGATATCTATCCTTTTATCTTCTAAAAAAATTTGTGAAAATCATTCCAAAAAAAACTCATGGAAATCATAATAGATAGAGAGGGAATGACAATGTAAAGGTTCGAACATAGAATGACACTACGAAAATAATCATAAAATTGTGATTGTGTGTTCTATCAATCTTGAACTGAGTGACAAACTTAAACTTACAGTAACTATATGAAAATAACATGATGATATACACATAATAATCTCAACCCTTTTTTCTCTGATTTTGTGTGTTTGATTTTGCAGCTGTGGCTGGCAAAGGGTTCATTGGTAAGGACATGGGGTTCGTTAACACCGCCGGTCCGGCGAAGCACCAGGCCGTCGCCTTCCGGTCAGGCTCCGATCTCTCTGTAATGTACGGCTGTTCTTTTGATGGCTATCAAGACACTCTCTACACCCACTCAAACCGTCAATTCTACCGCGACTGCGACATCAGTGGCACCATCGACTTCATCTTCGGCAACGCGGCGGTGGTGTTCCAGAACTGCAACATCCGGCCAAGGCAGCCGCTCCCCAACCAGTTCAACACCATCACGGCCCAAGGCAAAAAGGACATCAACCAGAACTCCGGCATTTCCATTCAGAAATGCACTTTCGCTGCCTACAACGACAGTCTCAGCGCCCCCACTTACTTGGGGCGGCCATGGAAGGAGTTCTCCACCACCGTGATTATGCAGTCCGGGATCGGTGGGTTCCTCCAGCCGGTCGGGTGGAAGGAATGGGTCAGCGGGCAGGACCCACCCAGCACCATATTTTACGGCGAGTATCAAAACAGTGGGCCGGGAGCGAACGTGGAGAAGAGGGTTAAATGGGCCGGTTACAGGCCCAGTCTTACCGACGTCGAGGCCGGAAAGTTCACCGTTGGGTCCTTCCTCCACGGCGAGGATTGGTTGCCGGCGGCCAGTGTAAAATTTGATACATCCTTGTGATCAAAGTCTCCCTTTAAGGGAACGACAACACATAGAATAAGAAAAATCACACGTTGAGAAGTTTAAATTAATGTAATTTTTTTTCCCATTTTTGTTGGGATTTTTCTTGCCCCGAGATATTCTTTGTTATAGCCAATTGTGAAGTTAGATTGGATTACGACGTCGTTGAATGTTTGCTTTTTAAGTTCAATATATAAATGCGTGAATTCTTGTGGATGGATAGACATGGACTGATTCTTCTTCATTTGGAGGCAAAA

mRNA sequence

GAGTTATTAAAACTTTTCAATCAACCTCCATTTAAAATAGTATCATTTTCTTAGTGATCCAAACGGTCCGACCTTCTAATAAATAATATATGCTTCAAATCAATCCAGTTATATTTATGTCAGACATTTTAAACCTAGAGAAATTTTTTTAGTTTTTTTAGTATAACAAATAAGATGGGAAAATTCGAACTCATGTACCAATTATCGTTAAGATAAGCTAATGCTGACTATGACTGGTAGCACGACGATAAAATAAAGAAGAAACCAAGAAAACGAAAAAAAAAAATGGGGTCAGCAATGTGAATTTGACTGGAACACATGATTAGGAAGATTTATTCAAAATGTTTAAAAAAAAATAGTACGATGGATTACACATACGACATGCCATTTGCTTGTCCAACACTACATTACACATCTGACCTAATACGCGTAACCAATTCATACAAATAACTTAATTAAATTAATTAATCTAATTAATTTTGCTTTTCCCTCTTCTCTTCCCTTCGCGTTTGAAAGCCCCCCAATATGTTCGATTCCTCCACTCGCATTTCTCCATCGCCACGCATTTTCCTTTCCAAATCCCCAAACCTTCTCTCTCTGCAACTCTCAAATCCATGGATTCCGTCCAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAGCATCAAGCCTTCCGTCGGAAAACGCGCCGCCGACTCATCATTCTTCTTCTCTCCATCGTTCTCCTAATCGCCGTTGTCATCGGTGCCGTCGCCGGCATTCTAATCCGTAACCGGAACTCCTCCTCCTCCCCGCCCACCACCGCCGGTCCTCCCACAGAGCTCACTCCCGTCGCCTCGCTCAAAAGTCTCTGCAGCGTCACTCAGTATCCCAGCTCCTGCCTCTCCTCTCTCGCAAACTCCAACACCACCGATCCCGAGATTCTGTTCAAGCTTTCTCTCCGCGTCGCCTCCGATGCGCTCTCCAAACTCGCCGATTACTCCTCCACCCTCGCTTCCACTACCGATGACCCGAAAATCAGAGACGCAATCGGAATCTGCCGGAGCGTTTTCGAAGACGCCATTGACACTCTGAACGACACCGTTTCGTCCATGGAAGTGGATCGCCGTGGCCAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAGAACCTGGCTCAGCACCGCCATCACCGACCAGGAGACCTGCCTCGACGCCGTCCGAGATCTCAACCGGACGGCGGTTCTAGAAGATTTACAAGCAGCAATGGGGAACTCTACCGAATTCGCCAGCAATAGCTTAGCGATTGTGACTAAAATTCTGGCCGTGTTGGCCAATTTCAACATCCCAATCCACCGGAAGCTGATGGGGTTCCCGGAGTGGGTGAGCCCCGGCGACCGGAGGCTTCTGGAGGAGAACAATGTGACGACGCACGTGACGGTGTCGAAAGATGGGAAAGGGGACTTCTCGACGATCAAAGAGGCCGTAGCGGCGGTGCCAAAGAAGAGCAAAGAAAGATTCGTCATATATGTAAAAGAGGGAGTATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTATGGAGATGGCAGAACCAAAACCATTGTTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGCTGTGGCTGGCAAAGGGTTCATTGGTAAGGACATGGGGTTCGTTAACACCGCCGGTCCGGCGAAGCACCAGGCCGTCGCCTTCCGGTCAGGCTCCGATCTCTCTGTAATGTACGGCTGTTCTTTTGATGGCTATCAAGACACTCTCTACACCCACTCAAACCGTCAATTCTACCGCGACTGCGACATCAGTGGCACCATCGACTTCATCTTCGGCAACGCGGCGGTGGTGTTCCAGAACTGCAACATCCGGCCAAGGCAGCCGCTCCCCAACCAGTTCAACACCATCACGGCCCAAGGCAAAAAGGACATCAACCAGAACTCCGGCATTTCCATTCAGAAATGCACTTTCGCTGCCTACAACGACAGTCTCAGCGCCCCCACTTACTTGGGGCGGCCATGGAAGGAGTTCTCCACCACCGTGATTATGCAGTCCGGGATCGGTGGGTTCCTCCAGCCGGTCGGGTGGAAGGAATGGGTCAGCGGGCAGGACCCACCCAGCACCATATTTTACGGCGAGTATCAAAACAGTGGGCCGGGAGCGAACGTGGAGAAGAGGGTTAAATGGGCCGGTTACAGGCCCAGTCTTACCGACGTCGAGGCCGGAAAGTTCACCGTTGGGTCCTTCCTCCACGGCGAGGATTGGTTGCCGGCGGCCAGTGTAAAATTTGATACATCCTTGTGATCAAAGTCTCCCTTTAAGGGAACGACAACACATAGAATAAGAAAAATCACACGTTGAGAAGTTTAAATTAATGTAATTTTTTTTCCCATTTTTGTTGGGATTTTTCTTGCCCCGAGATATTCTTTGTTATAGCCAATTGTGAAGTTAGATTGGATTACGACGTCGTTGAATGTTTGCTTTTTAAGTTCAATATATAAATGCGTGAATTCTTGTGGATGGATAGACATGGACTGATTCTTCTTCATTTGGAGGCAAAA

Coding sequence (CDS)

ATGGATTCCGTCCAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAGCATCAAGCCTTCCGTCGGAAAACGCGCCGCCGACTCATCATTCTTCTTCTCTCCATCGTTCTCCTAATCGCCGTTGTCATCGGTGCCGTCGCCGGCATTCTAATCCGTAACCGGAACTCCTCCTCCTCCCCGCCCACCACCGCCGGTCCTCCCACAGAGCTCACTCCCGTCGCCTCGCTCAAAAGTCTCTGCAGCGTCACTCAGTATCCCAGCTCCTGCCTCTCCTCTCTCGCAAACTCCAACACCACCGATCCCGAGATTCTGTTCAAGCTTTCTCTCCGCGTCGCCTCCGATGCGCTCTCCAAACTCGCCGATTACTCCTCCACCCTCGCTTCCACTACCGATGACCCGAAAATCAGAGACGCAATCGGAATCTGCCGGAGCGTTTTCGAAGACGCCATTGACACTCTGAACGACACCGTTTCGTCCATGGAAGTGGATCGCCGTGGCCAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAGAACCTGGCTCAGCACCGCCATCACCGACCAGGAGACCTGCCTCGACGCCGTCCGAGATCTCAACCGGACGGCGGTTCTAGAAGATTTACAAGCAGCAATGGGGAACTCTACCGAATTCGCCAGCAATAGCTTAGCGATTGTGACTAAAATTCTGGCCGTGTTGGCCAATTTCAACATCCCAATCCACCGGAAGCTGATGGGGTTCCCGGAGTGGGTGAGCCCCGGCGACCGGAGGCTTCTGGAGGAGAACAATGTGACGACGCACGTGACGGTGTCGAAAGATGGGAAAGGGGACTTCTCGACGATCAAAGAGGCCGTAGCGGCGGTGCCAAAGAAGAGCAAAGAAAGATTCGTCATATATGTAAAAGAGGGAGTATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTATGGAGATGGCAGAACCAAAACCATTGTTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGCTGTGGCTGGCAAAGGGTTCATTGGTAAGGACATGGGGTTCGTTAACACCGCCGGTCCGGCGAAGCACCAGGCCGTCGCCTTCCGGTCAGGCTCCGATCTCTCTGTAATGTACGGCTGTTCTTTTGATGGCTATCAAGACACTCTCTACACCCACTCAAACCGTCAATTCTACCGCGACTGCGACATCAGTGGCACCATCGACTTCATCTTCGGCAACGCGGCGGTGGTGTTCCAGAACTGCAACATCCGGCCAAGGCAGCCGCTCCCCAACCAGTTCAACACCATCACGGCCCAAGGCAAAAAGGACATCAACCAGAACTCCGGCATTTCCATTCAGAAATGCACTTTCGCTGCCTACAACGACAGTCTCAGCGCCCCCACTTACTTGGGGCGGCCATGGAAGGAGTTCTCCACCACCGTGATTATGCAGTCCGGGATCGGTGGGTTCCTCCAGCCGGTCGGGTGGAAGGAATGGGTCAGCGGGCAGGACCCACCCAGCACCATATTTTACGGCGAGTATCAAAACAGTGGGCCGGGAGCGAACGTGGAGAAGAGGGTTAAATGGGCCGGTTACAGGCCCAGTCTTACCGACGTCGAGGCCGGAAAGTTCACCGTTGGGTCCTTCCTCCACGGCGAGGATTGGTTGCCGGCGGCCAGTGTAAAATTTGATACATCCTTGTGA

Protein sequence

MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSSPPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKLADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLRTWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIPIHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIYVKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKDMGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLGRPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAGYRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL
Homology
BLAST of MC08g0884 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 1.5e-210
Identity = 370/585 (63.25%), Postives = 456/585 (77.95%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MD+++SFKGYGKV+ELE QA+ +KTR+RLII+ +S ++LIAV+I AVAG++I NRNS SS
Sbjct: 1   MDTIKSFKGYGKVNELEQQAYEKKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSC---LSSLANSNTTDPEILFKLSLRVASDAL 120
           P + + P TEL+P ASLK++C  T+YPSSC   +SSL  SNTTDPE+LFKLSLRVA D L
Sbjct: 61  PSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDEL 120

Query: 121 SKLADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIE 180
           S     S   A+   D +++ AI +C SVF DA+D LND++S++       +  S + + 
Sbjct: 121 SSFP--SKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISAL--GTVAGRIASSASVS 180

Query: 181 DLRTWLSTAITDQETCLDAVRDLNRTA---VLEDLQAAMGNSTEFASNSLAIVTKILAVL 240
           ++ TWLS A+TDQ+TCLDAV +LN TA    L++++ AM NSTEFASNSLAIVTKIL +L
Sbjct: 181 NVETWLSAALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLL 240

Query: 241 ANFNIPI-HRKLMGFPEWVSPGDRRLLEE--NNVTTHVTVSKDGKGDFSTIKEAVAAVPK 300
           + F  PI HR+L+GFPEW+   +RRLLEE  N+ T    V+KDG G F TI EA+  V K
Sbjct: 241 SRFETPIHHRRLLGFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKK 300

Query: 301 KSKERFVIYVKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAV 360
           KS+ERF +YVKEG Y EN+ LDK+ WNVM+YGDG+ KT V G  NF+DGTPTF TATFAV
Sbjct: 301 KSEERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAV 360

Query: 361 AGKGFIGKDMGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCD 420
            GKGFI KD+GFVN AG +KHQAVA RSGSD SV + CSFDG+QDTLY HSNRQFYRDCD
Sbjct: 361 KGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCD 420

Query: 421 ISGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYND 480
           I+GTIDFIFGNAAVVFQ+C I PRQPLPNQFNTITAQGKKD NQN+GI IQK T   + +
Sbjct: 421 ITGTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGN 480

Query: 481 SLSAPTYLGRPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGAN 540
           +L+APTYLGRPWK+FSTTVIMQS IG  L PVGW  WV   +PP+TIFY EYQNSGPGA+
Sbjct: 481 NLTAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGAD 540

Query: 541 VEKRVKWAGYRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 577
           V +RVKWAGY+P++TD  A +FTV SF+ G +WLP A+V+FD++L
Sbjct: 541 VSQRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of MC08g0884 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 5.0e-198
Identity = 347/590 (58.81%), Postives = 443/590 (75.08%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSV SFKGYGKVDE +  A ++KTR+RL++L +S+V+LIAV+I AV   ++    + S+
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNEST 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSS---LANSNTTDPEILFKLSLRVASDAL 120
           P     PP ELTP  SLK++CSVT++P SC+SS   L +SNTTDPE LFKLSL+V  D L
Sbjct: 61  P----SPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 121 SKLADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIE 180
             ++D    L+  T+D +I+ A+ +C  + EDA+D LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 181 DLRTWLSTAITDQETCLDAVRDLNR-------TAVLEDLQAAMGNSTEFASNSLAIVTKI 240
           DL+TWLS  +TD ETC D++ +L +       + + ++L++AM  STEF SNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 241 LAVLANFNIPIHRK----------LMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFS 300
           L+ L++  IPIHR+           + F +W     RRLL+   +   VTV+ DG GD  
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 301 TIKEAVAAVPKKSKERFVIYVKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDG 360
           T+ EAVA VPKKS + FVIYVK G Y ENV++DKSKWNVM+YGDG+ KTI+SG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 361 TPTFSTATFAVAGKGFIGKDMGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYT 420
           TPT+ TATFA+ GKGFI KD+G +NTAG AKHQAVAFRSGSD SV Y CSFDG+QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 421 HSNRQFYRDCDISGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 480
           HSNRQFYRDCD++GTIDFIFG+AAVVFQ C I PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 481 IQKCTFAAYNDSLSAPTYLGRPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFY 540
           IQ+CT +A N ++ APTYLGRPWKEFSTTVIM++ IG  ++P GW  WVSG DPP++I Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 541 GEYQNSGPGANVEKRVKWAGYRPSLTDVEAGKFTVGSFLHGEDWLPAASV 571
           GEY+N+GPG++V +RVKWAGY+P ++D EA KFTV + LHG DW+PA  V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

BLAST of MC08g0884 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 8.9e-163
Identity = 297/537 (55.31%), Postives = 382/537 (71.14%), Query Frame = 0

Query: 44  IGAVAGILIRNRNSSSSPPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLA----NSNT 103
           +  +A   I  R+   SP     P    TP + L+++C+VT YP+SC+SS++    +  T
Sbjct: 428 LAIIAKFPIHERHGVQSPRLRKSP--HPTPSSVLRTVCNVTNYPASCISSISKLPLSKTT 487

Query: 104 TDPEILFKLSLRVASDALSKLADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVS 163
           TDP++LF+LSL+V  D L+ +      LA  T+D  ++ A+ +C  VF+ A+D++NDT+S
Sbjct: 488 TDPKVLFRLSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTIS 547

Query: 164 SM-EVDRRGQKFLSPSRIEDLRTWLSTAITDQETCLDAVRDLN-RTAVLEDLQAAMGNST 223
           S+ EV   G+K L+ S I DL TWLS+A+TD  TC D + + N  + + + L++AM NST
Sbjct: 548 SLDEVISGGKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNST 607

Query: 224 EFASNSLAIVTKILAVLANFNIPIH-RKLM---GFPEWVSPGDRRLLEENNVTTHVTVSK 283
           EF SNSLAIV ++L   +   IP+  R+L+    FP WV PG RRLL+  N+T HVTV+ 
Sbjct: 608 EFTSNSLAIVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAA 667

Query: 284 DGKGDFSTIKEAVAAVPKKSKERFVIYVKEGVYEENVILDKSKWNVMMYGDGRTKTIVSG 343
           DG GD  T+ EAV  VPKK K  FVIYVK G Y ENV++ K KWNV +YGDGR KTI+SG
Sbjct: 668 DGSGDVRTVNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISG 727

Query: 344 RLNFIDGTPTFSTATFAVAGKGFIGKDMGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDG 403
             N +DG  TF+T+TFA  GKGF+ KDMG +NTAGP KHQAVAFRS SD SV Y CSFDG
Sbjct: 728 STNMVDGVRTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDG 787

Query: 404 YQDTLYTHSNRQFYRDCDISGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDI 463
           YQDTLYTHSNRQ+YR+CD++GT+DFIFG   VVFQ C+IRPRQPLPNQFNTITA+G ++ 
Sbjct: 788 YQDTLYTHSNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEA 847

Query: 464 NQNSGISIQKCTFAAYNDSLSAPTYLGRPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQD 523
           NQN+GISI +CT +  N +++A TYLGRPWK FS TVIMQS IG F+ P GW  W S  D
Sbjct: 848 NQNTGISIHQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYD 907

Query: 524 -PPSTIFYGEYQNSGPGANVEKRVKWAGYRPSLTDVEAGKFTVGSFLHGED-WLPAA 569
            PP TIFY EY+NSGPG+++ KRVKWAGY+P  +D EA +FTV  FL G+D W+P A
Sbjct: 908 PPPRTIFYREYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

BLAST of MC08g0884 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.7e-137
Identity = 276/578 (47.75%), Postives = 365/578 (63.15%), Query Frame = 0

Query: 10  YGKVDELEHQAFR--RKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSSPPTTAGP 69
           YG++DE E       RKT++R+ I+ +S ++L+ +V+GAV G   R+ NS   P    G 
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARD-NSKKPPTENNGE 63

Query: 70  PTELTPVASLKSLCSVTQYPSSC---LSSLANSNTTDPEILFKLSLRVASDALSKLADYS 129
           P  +    S+K+LC VT +   C   L S  N++ + PE LFK +++V    LSK+ D  
Sbjct: 64  PISV----SVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGF 123

Query: 130 STLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLRTWLS 189
           S       D     A+G C  +   A+D LN+T++S           S    +DLRTWLS
Sbjct: 124 SN--GEHMDNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRTWLS 183

Query: 190 TAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIPIHRK 249
           +  T QETC+DA+ + N+ ++    +  + NSTE  SN+LAI+T +  +         R 
Sbjct: 184 SVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRL 243

Query: 250 LMGFPEWVSPGD------RRLLEENNVTTHVT--VSKDGKGDFSTIKEAVAAVPKKSKER 309
           L      V   D      RRLLE  ++    T  V+KDG G + TI EA+A V +K+++ 
Sbjct: 244 LETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKP 303

Query: 310 FVIYVKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGF 369
            +IYVK+GVY ENV ++K+KWNV+M GDG++KTIVS  LNFIDGTPTF TATFAV GKGF
Sbjct: 304 TIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGF 363

Query: 370 IGKDMGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTI 429
           + +DMGF+NTAGPAKHQAVA    +DLSV Y C+ D +QDT+Y H+ RQFYRDC I GT+
Sbjct: 364 MARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTV 423

Query: 430 DFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAP 489
           DFIFGNAAVVFQ C I PR+P+  Q NTITAQG+KD NQN+GISI  CT    ++     
Sbjct: 424 DFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQ 483

Query: 490 TYLGRPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRV 549
           T+LGRPWK+FSTTVIM+S +  F+ P GW  W +G   P TIFY EY NSGPGA+ + RV
Sbjct: 484 TFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGASTKNRV 543

Query: 550 KWAGYRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDT 575
           KW G + SLT  EA KFTV  F+ G +WLPA  V F++
Sbjct: 544 KWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of MC08g0884 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 2.9e-129
Identity = 256/574 (44.60%), Postives = 355/574 (61.85%), Query Frame = 0

Query: 10  YGKVDELEHQAF--RRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSSPPTTAGP 69
           YGKVDE EH     RRKTR+ + I+ +S+V+L  +VIGAV G +   ++  +      G 
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 70  PTELTPVASLKSLCSVTQYPSSC---LSSLANSNTTDPEILFKLSLRVASDALSKLADYS 129
              +    S+K++C VT +   C   L S  N+++ +PE LF+ ++++    +SK     
Sbjct: 65  SISV----SVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI--- 124

Query: 130 STLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLRTWLS 189
           +  +S+  D K    +  C  + +  ID LN+T++S            P  ++DLRTWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSS----NGDVTVPELVDDLRTWLS 184

Query: 190 TAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIPIHRK 249
           +A T Q TC++ +    R       ++ + NSTE  SN+LAI+T +  +  +F +     
Sbjct: 185 SAGTYQRTCVETLAPDMRPFG----ESHLKNSTELTSNALAIITWLGKIADSFKLRRRLL 244

Query: 250 LMGFPEWVSPGDRRLLEENNV--TTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIYVK 309
                E      RRLL+  ++     + V+KDG G + TIK A+  VP+KS++R +IYVK
Sbjct: 245 TTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVK 304

Query: 310 EGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKDMG 369
           +GVY ENV ++K  WNV++ GDG +K+IVSGRLN IDGTPTF TATFAV GKGF+ +DMG
Sbjct: 305 KGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMG 364

Query: 370 FVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIFGN 429
           F+NTAGP+KHQAVA    +DL+  Y C+ + YQDTLY H+ RQFYR+C I GT+DFIFGN
Sbjct: 365 FINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGN 424

Query: 430 AAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLGRP 489
           +A V Q+C I PR+P+  Q NTITAQG+ D N N+GISI +C  +   D     T+LGRP
Sbjct: 425 SASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRP 484

Query: 490 WKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAGYR 549
           WK FSTTVIM S + GF+   GW  W +G   P TIFYGEY+N+GPGA+ + RVKW G R
Sbjct: 485 WKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLR 544

Query: 550 PSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 577
             L+  EA +FTV  F+ G  WLPA  V F + L
Sbjct: 545 -FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of MC08g0884 vs. NCBI nr
Match: XP_022152660.1 (pectinesterase 3 [Momordica charantia])

HSP 1 Score: 1136 bits (2939), Expect = 0.0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS
Sbjct: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
           PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL
Sbjct: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR
Sbjct: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP
Sbjct: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY
Sbjct: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF
Sbjct: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG
Sbjct: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL
Sbjct: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576

BLAST of MC08g0884 vs. NCBI nr
Match: XP_038877946.1 (pectinesterase 3 [Benincasa hispida])

HSP 1 Score: 1004 bits (2596), Expect = 0.0
Identity = 497/576 (86.28%), Postives = 544/576 (94.44%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MD+V+SFKGYGKVDELE QAFR+KTRRRLIILL+SIVLLIA+VI AV GI+I  RNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLVSIVLLIALVIAAVVGIVIHKRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
             T++ PPTELTP+ASLK+LCSVTQYPSSC SSL NSNTTDP  LFKLSLRVA+D+LSKL
Sbjct: 61  SATSSPPPTELTPLASLKTLCSVTQYPSSCQSSLENSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           +DY+S L S T+DPK++ AI ICRSVFEDAIDTLNDTVSSMEVDR  +KFLSPSRI+DL+
Sbjct: 121 SDYTSNLNSNTEDPKLKAAIEICRSVFEDAIDTLNDTVSSMEVDRHNEKFLSPSRIDDLK 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLST ITDQETCLDA+RDLN+T VL++LQ AM NSTEF SNSLAIVTKIL +LA+F+IP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILRLLADFDIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLMGFP+WVS GDRRLL+ENNVT HVTVSKDGKG+++TIK+AVAAVPKKSK+RF+IY
Sbjct: 241 IHRKLMGFPDWVSSGDRRLLQENNVTAHVTVSKDGKGNYTTIKDAVAAVPKKSKQRFIIY 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGF+NTAGPAKHQAVAFRSGSDLSV+ GCSFDGYQDTLY HSNRQFYRDCDI+GTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVISGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTF+AYND+L+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDNLAAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQS IGGFLQPVGWKEWVSGQDPPS+IFYGEYQNSGPG+NV+KRVKWAG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWAG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           YRPSLTD EAGKFTVG+FL+GEDWLPA SVKFDTSL
Sbjct: 541 YRPSLTDAEAGKFTVGTFLNGEDWLPATSVKFDTSL 576

BLAST of MC08g0884 vs. NCBI nr
Match: XP_023528356.1 (pectinesterase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1004 bits (2596), Expect = 0.0
Identity = 496/576 (86.11%), Postives = 539/576 (93.58%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSV+SFKGYGKVDELEHQAFR+KTRRRLIILLLS+VLLIA+VIGAV GI+I  RNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSVVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
             T++ PPTEL P ASLK+LCSVTQYPSSC SSL NSNTTDP  LFKLSLRVA+D++SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           +DY+S L S+T D K++ AIGIC SVF+DAIDTLNDTVSSMEVDR G+KFLSPSRIEDL+
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLST ITDQETCLDA+RDLN+TAVLE+LQA M NSTEF SNSLAIVTKIL +LA+FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLM FPEW+SPGDRRLL+ENNVT H TVSKDG GDF+TIKEAVAAVPKKSKERF+IY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTAHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGF+NTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLY HSNRQFYR+CDI+GTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTF+AYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQS IG FLQP+GWKEWVSGQDPPS+IFYGEYQNSGPG+NVEKRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGAFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           YRPSLTD EA KFTV +FL+GEDWLPA SVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDTSL 576

BLAST of MC08g0884 vs. NCBI nr
Match: XP_022956093.1 (pectinesterase 3-like [Cucurbita moschata] >KAG6581793.1 Pectinesterase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1004 bits (2596), Expect = 0.0
Identity = 496/576 (86.11%), Postives = 540/576 (93.75%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSV+SFKGYGKVDELEHQAFR+KTRRRLIILLLSIVLLIA+VIGAV GI+I  RNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
             T++ PPTEL P ASLK+LCSVTQYPSSC SSL NSNTTDP  LFKLSLRVA+D++SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           +DY+S L S+T D K++ AIGIC SVF+DAIDTLNDTVSSMEVDR G+KFLSPSRIEDL+
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLST ITDQETCLDA+RDLN+TAVLE+LQA M NSTEF SNSLAIVTKIL +LA+FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLM FPEW+SPGDRRLL+ENNVT H TVSKDG GDF+TIKEAVAAVPKKSKERF+IY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGF+NTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLY HSNRQFYR+CDI+GTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTF+AYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQS IGGFLQP+GWKEWVSGQDPPS+IFYGEYQNSGPG+NV+KRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           YRPSLTD EA KFTV +FL+GE+WLPA SVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

BLAST of MC08g0884 vs. NCBI nr
Match: KAG7018244.1 (Pectinesterase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1003 bits (2593), Expect = 0.0
Identity = 495/576 (85.94%), Postives = 540/576 (93.75%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSV+SFKGYGKVDELEHQAFR+KTRRRLIILLLSIVLLIA+VIGAV GI+I  RNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
             T++ PPTEL P ASLK+LCSVTQYPSSC SSL NSNTTDP  LFKLSLRVA+D++SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           +DY+S L S+T D K++ AIGIC SVF+DAIDTLNDTVSSMEVDR G+KFLSPSRIEDL+
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLST ITDQETCLDA+RDLN+TAVLE+LQA M NSTEF SNSLAIVTKIL +LA+FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLM FPEW+SPGDRRLL+ENNVT H TVSKDG GDF+TIKEAVAAVPKKSKERF+IY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGF+NTAGPAKHQAVAFRSGSDLSV+YGCSFDGYQDTLY HSNRQFYR+CDI+GTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVLYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTF+AYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQS IGGFLQP+GWKEWVSGQDPPS+IFYGEYQNSGPG+NV+KRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           YRPSLTD EA KFTV +FL+GE+WLPA SVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

BLAST of MC08g0884 vs. ExPASy TrEMBL
Match: A0A6J1DEK2 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020324 PE=3 SV=1)

HSP 1 Score: 1136 bits (2939), Expect = 0.0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS
Sbjct: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
           PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL
Sbjct: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR
Sbjct: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP
Sbjct: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY
Sbjct: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF
Sbjct: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG
Sbjct: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL
Sbjct: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576

BLAST of MC08g0884 vs. ExPASy TrEMBL
Match: A0A6J1GVT9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1)

HSP 1 Score: 1004 bits (2596), Expect = 0.0
Identity = 496/576 (86.11%), Postives = 540/576 (93.75%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSV+SFKGYGKVDELEHQAFR+KTRRRLIILLLSIVLLIA+VIGAV GI+I  RNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
             T++ PPTEL P ASLK+LCSVTQYPSSC SSL NSNTTDP  LFKLSLRVA+D++SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           +DY+S L S+T D K++ AIGIC SVF+DAIDTLNDTVSSMEVDR G+KFLSPSRIEDL+
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLST ITDQETCLDA+RDLN+TAVLE+LQA M NSTEF SNSLAIVTKIL +LA+FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLM FPEW+SPGDRRLL+ENNVT H TVSKDG GDF+TIKEAVAAVPKKSKERF+IY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGF+NTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLY HSNRQFYR+CDI+GTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTF+AYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQS IGGFLQP+GWKEWVSGQDPPS+IFYGEYQNSGPG+NV+KRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           YRPSLTD EA KFTV +FL+GE+WLPA SVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

BLAST of MC08g0884 vs. ExPASy TrEMBL
Match: A0A6J1IZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1)

HSP 1 Score: 1002 bits (2591), Expect = 0.0
Identity = 497/576 (86.28%), Postives = 537/576 (93.23%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSV+SFKGYGKVDELEHQAFR+KTRRRLIILLLSIVLLIA+VIGAV GI+I  RNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
             T++ PPTEL P ASLK+LCSVTQYPSSC SSL NSNTTDP  LFKLSLRVA+D++SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           +DY+S L S+T D K++ AIGIC SVF+DAIDTLNDTVSSMEVDR G+KFLSPSRIEDL+
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICTSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLST ITDQETCLDA+RDLN TAVLE+LQA M NSTEF SNSLAIVTKIL +LANFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNHTAVLENLQAVMANSTEFTSNSLAIVTKILGLLANFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLM FPEWVSPGDRRLL+ENNVT H TVSKDG  +++TIKEAVAAVPKKSKERF+IY
Sbjct: 241 IHRKLMAFPEWVSPGDRRLLQENNVTAHATVSKDGTEEYTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDGRT+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGF+NTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLY HSNRQFYR+CDI+GTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAA VFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTF+AYNDSL+APTYLG
Sbjct: 421 GNAAAVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQS IGGFLQPVGWKEWVSGQDPPS+IFYGEYQNSGPG+NVEKRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           YRPSLTD EA KFTV +FL+GEDWLPA SVKFD+SL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDSSL 576

BLAST of MC08g0884 vs. ExPASy TrEMBL
Match: A0A1S3CBU9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1)

HSP 1 Score: 994 bits (2571), Expect = 0.0
Identity = 490/576 (85.07%), Postives = 537/576 (93.23%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MD+V+SFKGYGKVDELE QAFR+KTRRRLII+ +S+VLLIA++IGAV GI++  RNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
             TT+ PPTELTP ASLK+LCSVTQYPSSC SSL NSNTTDP  LFKLSLRVA+D+LSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           +DY+S   S T DPK++ AI IC+SVFEDAIDTLNDT+SSMEVDR  +KFLSPSRIEDL+
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLST ITDQETCLDA+RDLN+T VL+DLQ AM NSTEF SNSLAIVTKIL +LA+FNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLMG PEWVS GDRRLL+ENNVT HVTVSKDGKGD++TIK+AVAAVPKKSKERF+I+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGF+NTAGPAKHQAVAFRSGSDLSVM GCSFDGYQDTLY HSNRQFYRDCDI+GTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTF+AYN+SLSAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIMQS IGGFL PVGWKEWVSGQDPPS+IFYGEYQNSGPG+NV+KRV+WAG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           Y+PSLTD EAGKFTVG+FL+GEDWLPA +V FDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of MC08g0884 vs. ExPASy TrEMBL
Match: A0A0A0L956 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1)

HSP 1 Score: 992 bits (2565), Expect = 0.0
Identity = 488/576 (84.72%), Postives = 536/576 (93.06%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MD+V+SFKGYGKVDELE QAFR+KTRRRLII+ +S++LLIA+++GAV GI++  RNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLANSNTTDPEILFKLSLRVASDALSKL 120
             TT+ PPTELTP ASLK+LCSVTQYPSSC SSL NSNTTDP  LFKLSLRVA+D+LSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLR 180
           +DY+S   STT DPK+  AI ICRSVFEDAIDTLNDTVSSMEVDR  +KFLSPSRIEDL+
Sbjct: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIP 240
           TWLST ITDQETCLDA+RDLN+T VL+DLQ AM NSTEF SNSLAIVTKIL +LA+FNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIY 300
           IHRKLMG PEWVS GDRRLL+ENNVT HVTVSKDGKG ++TI++AVAAVPKKSKERF+I+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300

Query: 301 VKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIF 420
           MGF+NTAGPAKHQAVAFRSGSDLSVM GCSFDGYQDTLY HSNRQFYRDCDI+GTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTF+AYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480

Query: 481 RPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAG 540
           RPWKEFSTTVIM+S IGGFL PVGWKEWVSGQDPPS+IFYGEYQNSGPG+NV+KRV+WAG
Sbjct: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 576
           Y+PSLTD EAG+FTVG+FL+GEDWLPA +V FDTSL
Sbjct: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576

BLAST of MC08g0884 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 692.2 bits (1785), Expect = 3.6e-199
Identity = 347/590 (58.81%), Postives = 443/590 (75.08%), Query Frame = 0

Query: 1   MDSVQSFKGYGKVDELEHQAFRRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSS 60
           MDSV SFKGYGKVDE +  A ++KTR+RL++L +S+V+LIAV+I AV   ++    + S+
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNEST 60

Query: 61  PPTTAGPPTELTPVASLKSLCSVTQYPSSCLSS---LANSNTTDPEILFKLSLRVASDAL 120
           P     PP ELTP  SLK++CSVT++P SC+SS   L +SNTTDPE LFKLSL+V  D L
Sbjct: 61  P----SPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 121 SKLADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIE 180
             ++D    L+  T+D +I+ A+ +C  + EDA+D LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 181 DLRTWLSTAITDQETCLDAVRDLNR-------TAVLEDLQAAMGNSTEFASNSLAIVTKI 240
           DL+TWLS  +TD ETC D++ +L +       + + ++L++AM  STEF SNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 241 LAVLANFNIPIHRK----------LMGFPEWVSPGDRRLLEENNVTTHVTVSKDGKGDFS 300
           L+ L++  IPIHR+           + F +W     RRLL+   +   VTV+ DG GD  
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 301 TIKEAVAAVPKKSKERFVIYVKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDG 360
           T+ EAVA VPKKS + FVIYVK G Y ENV++DKSKWNVM+YGDG+ KTI+SG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 361 TPTFSTATFAVAGKGFIGKDMGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYT 420
           TPT+ TATFA+ GKGFI KD+G +NTAG AKHQAVAFRSGSD SV Y CSFDG+QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 421 HSNRQFYRDCDISGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 480
           HSNRQFYRDCD++GTIDFIFG+AAVVFQ C I PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 481 IQKCTFAAYNDSLSAPTYLGRPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFY 540
           IQ+CT +A N ++ APTYLGRPWKEFSTTVIM++ IG  ++P GW  WVSG DPP++I Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 541 GEYQNSGPGANVEKRVKWAGYRPSLTDVEAGKFTVGSFLHGEDWLPAASV 571
           GEY+N+GPG++V +RVKWAGY+P ++D EA KFTV + LHG DW+PA  V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

BLAST of MC08g0884 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 575.1 bits (1481), Expect = 6.3e-164
Identity = 297/537 (55.31%), Postives = 382/537 (71.14%), Query Frame = 0

Query: 44  IGAVAGILIRNRNSSSSPPTTAGPPTELTPVASLKSLCSVTQYPSSCLSSLA----NSNT 103
           +  +A   I  R+   SP     P    TP + L+++C+VT YP+SC+SS++    +  T
Sbjct: 428 LAIIAKFPIHERHGVQSPRLRKSP--HPTPSSVLRTVCNVTNYPASCISSISKLPLSKTT 487

Query: 104 TDPEILFKLSLRVASDALSKLADYSSTLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVS 163
           TDP++LF+LSL+V  D L+ +      LA  T+D  ++ A+ +C  VF+ A+D++NDT+S
Sbjct: 488 TDPKVLFRLSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTIS 547

Query: 164 SM-EVDRRGQKFLSPSRIEDLRTWLSTAITDQETCLDAVRDLN-RTAVLEDLQAAMGNST 223
           S+ EV   G+K L+ S I DL TWLS+A+TD  TC D + + N  + + + L++AM NST
Sbjct: 548 SLDEVISGGKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNST 607

Query: 224 EFASNSLAIVTKILAVLANFNIPIH-RKLM---GFPEWVSPGDRRLLEENNVTTHVTVSK 283
           EF SNSLAIV ++L   +   IP+  R+L+    FP WV PG RRLL+  N+T HVTV+ 
Sbjct: 608 EFTSNSLAIVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAA 667

Query: 284 DGKGDFSTIKEAVAAVPKKSKERFVIYVKEGVYEENVILDKSKWNVMMYGDGRTKTIVSG 343
           DG GD  T+ EAV  VPKK K  FVIYVK G Y ENV++ K KWNV +YGDGR KTI+SG
Sbjct: 668 DGSGDVRTVNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISG 727

Query: 344 RLNFIDGTPTFSTATFAVAGKGFIGKDMGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDG 403
             N +DG  TF+T+TFA  GKGF+ KDMG +NTAGP KHQAVAFRS SD SV Y CSFDG
Sbjct: 728 STNMVDGVRTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDG 787

Query: 404 YQDTLYTHSNRQFYRDCDISGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDI 463
           YQDTLYTHSNRQ+YR+CD++GT+DFIFG   VVFQ C+IRPRQPLPNQFNTITA+G ++ 
Sbjct: 788 YQDTLYTHSNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEA 847

Query: 464 NQNSGISIQKCTFAAYNDSLSAPTYLGRPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQD 523
           NQN+GISI +CT +  N +++A TYLGRPWK FS TVIMQS IG F+ P GW  W S  D
Sbjct: 848 NQNTGISIHQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYD 907

Query: 524 -PPSTIFYGEYQNSGPGANVEKRVKWAGYRPSLTDVEAGKFTVGSFLHGED-WLPAA 569
            PP TIFY EY+NSGPG+++ KRVKWAGY+P  +D EA +FTV  FL G+D W+P A
Sbjct: 908 PPPRTIFYREYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

BLAST of MC08g0884 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 491.1 bits (1263), Expect = 1.2e-138
Identity = 276/578 (47.75%), Postives = 365/578 (63.15%), Query Frame = 0

Query: 10  YGKVDELEHQAFR--RKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSSPPTTAGP 69
           YG++DE E       RKT++R+ I+ +S ++L+ +V+GAV G   R+ NS   P    G 
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARD-NSKKPPTENNGE 63

Query: 70  PTELTPVASLKSLCSVTQYPSSC---LSSLANSNTTDPEILFKLSLRVASDALSKLADYS 129
           P  +    S+K+LC VT +   C   L S  N++ + PE LFK +++V    LSK+ D  
Sbjct: 64  PISV----SVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGF 123

Query: 130 STLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLRTWLS 189
           S       D     A+G C  +   A+D LN+T++S           S    +DLRTWLS
Sbjct: 124 SN--GEHMDNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRTWLS 183

Query: 190 TAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIPIHRK 249
           +  T QETC+DA+ + N+ ++    +  + NSTE  SN+LAI+T +  +         R 
Sbjct: 184 SVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRL 243

Query: 250 LMGFPEWVSPGD------RRLLEENNVTTHVT--VSKDGKGDFSTIKEAVAAVPKKSKER 309
           L      V   D      RRLLE  ++    T  V+KDG G + TI EA+A V +K+++ 
Sbjct: 244 LETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKP 303

Query: 310 FVIYVKEGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGF 369
            +IYVK+GVY ENV ++K+KWNV+M GDG++KTIVS  LNFIDGTPTF TATFAV GKGF
Sbjct: 304 TIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGF 363

Query: 370 IGKDMGFVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTI 429
           + +DMGF+NTAGPAKHQAVA    +DLSV Y C+ D +QDT+Y H+ RQFYRDC I GT+
Sbjct: 364 MARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTV 423

Query: 430 DFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAP 489
           DFIFGNAAVVFQ C I PR+P+  Q NTITAQG+KD NQN+GISI  CT    ++     
Sbjct: 424 DFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQ 483

Query: 490 TYLGRPWKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRV 549
           T+LGRPWK+FSTTVIM+S +  F+ P GW  W +G   P TIFY EY NSGPGA+ + RV
Sbjct: 484 TFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGASTKNRV 543

Query: 550 KWAGYRPSLTDVEAGKFTVGSFLHGEDWLPAASVKFDT 575
           KW G + SLT  EA KFTV  F+ G +WLPA  V F++
Sbjct: 544 KWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of MC08g0884 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 463.8 bits (1192), Expect = 2.1e-130
Identity = 256/574 (44.60%), Postives = 355/574 (61.85%), Query Frame = 0

Query: 10  YGKVDELEHQAF--RRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSSPPTTAGP 69
           YGKVDE EH     RRKTR+ + I+ +S+V+L  +VIGAV G +   ++  +      G 
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 70  PTELTPVASLKSLCSVTQYPSSC---LSSLANSNTTDPEILFKLSLRVASDALSKLADYS 129
              +    S+K++C VT +   C   L S  N+++ +PE LF+ ++++    +SK     
Sbjct: 65  SISV----SVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI--- 124

Query: 130 STLASTTDDPKIRDAIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLRTWLS 189
           +  +S+  D K    +  C  + +  ID LN+T++S            P  ++DLRTWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSS----NGDVTVPELVDDLRTWLS 184

Query: 190 TAITDQETCLDAVRDLNRTAVLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIPIHRK 249
           +A T Q TC++ +    R       ++ + NSTE  SN+LAI+T +  +  +F +     
Sbjct: 185 SAGTYQRTCVETLAPDMRPFG----ESHLKNSTELTSNALAIITWLGKIADSFKLRRRLL 244

Query: 250 LMGFPEWVSPGDRRLLEENNV--TTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIYVK 309
                E      RRLL+  ++     + V+KDG G + TIK A+  VP+KS++R +IYVK
Sbjct: 245 TTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVK 304

Query: 310 EGVYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKDMG 369
           +GVY ENV ++K  WNV++ GDG +K+IVSGRLN IDGTPTF TATFAV GKGF+ +DMG
Sbjct: 305 KGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMG 364

Query: 370 FVNTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIFGN 429
           F+NTAGP+KHQAVA    +DL+  Y C+ + YQDTLY H+ RQFYR+C I GT+DFIFGN
Sbjct: 365 FINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGN 424

Query: 430 AAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYNDSLSAPTYLGRP 489
           +A V Q+C I PR+P+  Q NTITAQG+ D N N+GISI +C  +   D     T+LGRP
Sbjct: 425 SASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRP 484

Query: 490 WKEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAGYR 549
           WK FSTTVIM S + GF+   GW  W +G   P TIFYGEY+N+GPGA+ + RVKW G R
Sbjct: 485 WKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLR 544

Query: 550 PSLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 577
             L+  EA +FTV  F+ G  WLPA  V F + L
Sbjct: 545 -FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of MC08g0884 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 408.7 bits (1049), Expect = 7.9e-114
Identity = 237/573 (41.36%), Postives = 328/573 (57.24%), Query Frame = 0

Query: 22  RRKTRRRLIILLLSIVLLIAVVIGAVAGILIRNRNSSSSPPTTAGPPTELTPVASLKSLC 81
           ++KT+ +LI+  L+++++  V  G  AG  IR  +S  + P     PT+     ++   C
Sbjct: 27  QKKTKTKLILFTLAVLVVGVVCFGIFAG--IRAVDSGKTEPKLTRKPTQ-----AISRTC 86

Query: 82  SVTQYPSSCLSSLAN---SNTTDPEILFKLSLRVASDALSKLADYSSTLASTTDDPKIRD 141
           S + YP+ C+ +L +   S T D   L  +S        SK    SST+  T   P++R 
Sbjct: 87  SKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPPRVRS 146

Query: 142 AIGICRSVFEDAIDTLNDTVSSMEVDRRGQKFLSPSRIEDLRTWLSTAITDQETCLDAVR 201
           A   C  + +D++D L   +SS+ V    +         D+ TWLS+A+T+ +TC D   
Sbjct: 147 AYDSCLELLDDSVDALTRALSSVVVVSGDESH------SDVMTWLSSAMTNHDTCTDGFD 206

Query: 202 DLNRTA--VLEDLQAAMGNSTEFASNSLAIVTKILAVLANFNIPIHRKLMG------FPE 261
           ++      V + +  A+ + +E  SN LAI    +  L+   +  +RKL+G       P 
Sbjct: 207 EIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEELPN 266

Query: 262 WVSPGDRRLL--EENNVTTHVTVSKDGKGDFSTIKEAVAAVPKKSKERFVIYVKEGVY-E 321
           W+   DR LL    + +   +TVSKDG G F TI EA+   P+ S  RFVIYVK G Y E
Sbjct: 267 WLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEE 326

Query: 322 ENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKDMGFVNTA 381
           EN+ + + K N+M  GDG+ KT+++G  +  D   TF TATFA  G GFI +DM F N A
Sbjct: 327 ENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYA 386

Query: 382 GPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYTHSNRQFYRDCDISGTIDFIFGNAAVVF 441
           GPAKHQAVA R G D +V+Y C+  GYQD LY HSNRQF+R+C+I GT+DFIFGNAAV+ 
Sbjct: 387 GPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVIL 446

Query: 442 QNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFAAYND----SLSAPTYLGRPW 501
           Q+CNI  R+P+  Q  TITAQ +KD NQN+GISI  C   A  D      S PTYLGRPW
Sbjct: 447 QSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPW 506

Query: 502 KEFSTTVIMQSGIGGFLQPVGWKEWVSGQDPPSTIFYGEYQNSGPGANVEKRVKWAGYRP 561
           K +S  V M S +G  + P GW EW +G     +++YGEY N G G+ + +RVKW GY  
Sbjct: 507 KLYSRVVYMMSDMGDHIDPRGWLEW-NGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHV 566

Query: 562 SLTDVEAGKFTVGSFLHGEDWLPAASVKFDTSL 577
             + VEA KFTV  F+ G  WLP+  V F + L
Sbjct: 567 ITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q431111.5e-21063.25Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1[more]
Q438675.0e-19858.81Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1[more]
Q9LUL88.9e-16355.31Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3... [more]
Q9FF781.7e-13747.75Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3... [more]
Q9SG772.9e-12944.60Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_022152660.10.0100.00pectinesterase 3 [Momordica charantia][more]
XP_038877946.10.086.28pectinesterase 3 [Benincasa hispida][more]
XP_023528356.10.086.11pectinesterase 3-like [Cucurbita pepo subsp. pepo][more]
XP_022956093.10.086.11pectinesterase 3-like [Cucurbita moschata] >KAG6581793.1 Pectinesterase 3, parti... [more]
KAG7018244.10.085.94Pectinesterase 3 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1DEK20.0100.00Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020324 PE=3 SV=1[more]
A0A6J1GVT90.086.11Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1[more]
A0A6J1IZN00.086.28Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1[more]
A0A1S3CBU90.085.07Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1[more]
A0A0A0L9560.084.72Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G53840.13.6e-19958.81pectin methylesterase 1 [more]
AT3G14300.16.3e-16455.31pectinesterase family protein [more]
AT5G04960.11.2e-13847.75Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10710.12.1e-13044.60root hair specific 12 [more]
AT5G53370.17.9e-11441.36pectin methylesterase PCR fragment F [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 72..226
e-value: 1.3E-33
score: 127.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 77..226
e-value: 3.5E-25
score: 89.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 66..230
e-value: 2.5E-21
score: 73.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 262..576
e-value: 3.6E-115
score: 386.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 268..561
e-value: 2.1E-139
score: 463.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 75..231
e-value: 1.3E-31
score: 111.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 77..230
NoneNo IPR availablePANTHERPTHR31707:SF219PECTINESTERASE 1-RELATEDcoord: 9..573
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 9..573
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 79..230
e-value: 1.49096E-42
score: 147.97
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 412..421
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 267..572

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0884.1MC08g0884.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity