MC08g0855 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0855
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein BIG GRAIN 1-like B
LocationMC08: 6923708 .. 6924724 (-)
RNA-Seq ExpressionMC08g0855
SyntenyMC08g0855
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAATTTGTGGGATAAGCCGCAGCCGCTGCAGAGGGAAGCTCGAGATCACCGCCGCCGTCTCCGCCGCGAAAACCCATCTTTCTCCTCCTCCCTTCTCGACGCCATCTACCGTTCGATCGACGAATCCAACGGCCAGGGAGAAGAGCCGCAGCTGATTCTCTACAGAGAAACCACGATGACAAAACAGAGTATCGCCGCCGCGAGAACAGAGGACGCCGACGAAGCTCTGAAATTCCGGCGAGTCGAAAAGTGGATGGAGATGAAGAAGAAGAAAGTCGGATCAAACGTTCGCGATTTCACGAATTCCAGCTCCAGCTCGTCGGAATCCAGCTGCACCGCCGGCAGATTCTCCTCGTCGGAATCGGAATTCCTCTCACGGCCGAAGCCCATCAAAACCACTACAGAAATCCCGAAGCCGAAGCCGAAGCACGAAAATGGGTTCCTCAAAACCAAATCCAAAGCCTCAAAAATGTACCACGATTTGAAGAAAGTGAAACAGCCGATTTCCCCGGGAGCCCGCCTCGCCAATTTCCTCAATTCCCTTTTCAATGGCGGAACCCCAAAAACGAAGCAAAAACTTTCGAATTCTTCCGTACAATCGTCCAAATCCTTGCAGGGATCCACGTGCTCCTCTACGTCGTCGTTTTCAAGGTCCTGTTTGAGCAAAACGCCATCTTCGCGAGGGAACGCCGCCAAGAGATCGGTTCGATTCTGCCCTGTGAGTGTGATTGTGGACGAGGATTGCAGGCCCTGCGGCCATAAAATTCTGCACAAAATCGAACCCATTTTGAAGAAACCTTTGAAAACAGAGCAAGTAAAAACAGAGCATGATTTTGAAGACGACGATGAAGATTATGACGATGAGGATGATGATGGATTGAGTTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCGGTTATTGGAATCGAGAGGTACAGAGAAGAATTGCCGGTGTATGAAACTACAAATTTCAAAACCAATTGTGCCATTGCTAATGGTTTGGTTCTG

mRNA sequence

TTTAATTTGTGGGATAAGCCGCAGCCGCTGCAGAGGGAAGCTCGAGATCACCGCCGCCGTCTCCGCCGCGAAAACCCATCTTTCTCCTCCTCCCTTCTCGACGCCATCTACCGTTCGATCGACGAATCCAACGGCCAGGGAGAAGAGCCGCAGCTGATTCTCTACAGAGAAACCACGATGACAAAACAGAGTATCGCCGCCGCGAGAACAGAGGACGCCGACGAAGCTCTGAAATTCCGGCGAGTCGAAAAGTGGATGGAGATGAAGAAGAAGAAAGTCGGATCAAACGTTCGCGATTTCACGAATTCCAGCTCCAGCTCGTCGGAATCCAGCTGCACCGCCGGCAGATTCTCCTCGTCGGAATCGGAATTCCTCTCACGGCCGAAGCCCATCAAAACCACTACAGAAATCCCGAAGCCGAAGCCGAAGCACGAAAATGGGTTCCTCAAAACCAAATCCAAAGCCTCAAAAATGTACCACGATTTGAAGAAAGTGAAACAGCCGATTTCCCCGGGAGCCCGCCTCGCCAATTTCCTCAATTCCCTTTTCAATGGCGGAACCCCAAAAACGAAGCAAAAACTTTCGAATTCTTCCGTACAATCGTCCAAATCCTTGCAGGGATCCACGTGCTCCTCTACGTCGTCGTTTTCAAGGTCCTGTTTGAGCAAAACGCCATCTTCGCGAGGGAACGCCGCCAAGAGATCGGTTCGATTCTGCCCTGTGAGTGTGATTGTGGACGAGGATTGCAGGCCCTGCGGCCATAAAATTCTGCACAAAATCGAACCCATTTTGAAGAAACCTTTGAAAACAGAGCAAGTAAAAACAGAGCATGATTTTGAAGACGACGATGAAGATTATGACGATGAGGATGATGATGGATTGAGTTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCGGTTATTGGAATCGAGAGGTACAGAGAAGAATTGCCGGTGTATGAAACTACAAATTTCAAAACCAATTGTGCCATTGCTAATGGTTTGGTTCTG

Coding sequence (CDS)

TTTAATTTGTGGGATAAGCCGCAGCCGCTGCAGAGGGAAGCTCGAGATCACCGCCGCCGTCTCCGCCGCGAAAACCCATCTTTCTCCTCCTCCCTTCTCGACGCCATCTACCGTTCGATCGACGAATCCAACGGCCAGGGAGAAGAGCCGCAGCTGATTCTCTACAGAGAAACCACGATGACAAAACAGAGTATCGCCGCCGCGAGAACAGAGGACGCCGACGAAGCTCTGAAATTCCGGCGAGTCGAAAAGTGGATGGAGATGAAGAAGAAGAAAGTCGGATCAAACGTTCGCGATTTCACGAATTCCAGCTCCAGCTCGTCGGAATCCAGCTGCACCGCCGGCAGATTCTCCTCGTCGGAATCGGAATTCCTCTCACGGCCGAAGCCCATCAAAACCACTACAGAAATCCCGAAGCCGAAGCCGAAGCACGAAAATGGGTTCCTCAAAACCAAATCCAAAGCCTCAAAAATGTACCACGATTTGAAGAAAGTGAAACAGCCGATTTCCCCGGGAGCCCGCCTCGCCAATTTCCTCAATTCCCTTTTCAATGGCGGAACCCCAAAAACGAAGCAAAAACTTTCGAATTCTTCCGTACAATCGTCCAAATCCTTGCAGGGATCCACGTGCTCCTCTACGTCGTCGTTTTCAAGGTCCTGTTTGAGCAAAACGCCATCTTCGCGAGGGAACGCCGCCAAGAGATCGGTTCGATTCTGCCCTGTGAGTGTGATTGTGGACGAGGATTGCAGGCCCTGCGGCCATAAAATTCTGCACAAAATCGAACCCATTTTGAAGAAACCTTTGAAAACAGAGCAAGTAAAAACAGAGCATGATTTTGAAGACGACGATGAAGATTATGACGATGAGGATGATGATGGATTGAGTTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCGGTTATTGGAATCGAGAGGTACAGAGAAGAATTGCCGGTGTATGAAACTACAAATTTCAAAACCAATTGTGCCATTGCTAATGGTTTGGTTCTG

Protein sequence

FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSSESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL
Homology
BLAST of MC08g0855 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 4.4e-53
Identity = 165/374 (44.12%), Postives = 210/374 (56.15%), Query Frame = 0

Query: 16  DHRRRLR----RENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTE 75
           DH+ RLR      +PSFSS+LLD IYRSID+S+      +   ++    T+  ++A R +
Sbjct: 12  DHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRKTKHQNREDTR--VSANRRD 71

Query: 76  DADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSSESEF------- 135
           D + +   + +E                F +SSSSSS+SS     FSSSES++       
Sbjct: 72  DFNRSKNLKTIEPVF-------------FKHSSSSSSDSS----GFSSSESDYFYRRSKS 131

Query: 136 ---LSRPKPIKTTTE---------IPKPKPKHENG-FLKTKSKASKMYHDLKKVKQPISP 195
              +S PKPI+TT E          P    K E+G FLKTKSKA K+Y DLKKVKQPISP
Sbjct: 132 SPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKALKIYSDLKKVKQPISP 191

Query: 196 GARLANFLNSLFNGGTPKTKQKLSNSSVQSSKSLQG-----STCSSTSSFSRSCLSKTPS 255
           G RLA FLNS+F G     K    N++V S+ +        +TCSS SSFSRSCLSKTPS
Sbjct: 192 GGRLATFLNSIFTGAGNTKKLNKINTTVTSTTAAAAAASSTTTCSSASSFSRSCLSKTPS 251

Query: 256 SRGNAAKRSVRFCPVSVIVDEDCRPCGHK--------------ILHKIE----------- 315
           S    +KRSVRFCPV+VI DED     +K              I H +E           
Sbjct: 252 S-SEKSKRSVRFCPVNVIFDEDSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVI 311

Query: 316 PILKKPLKTEQVKTEHDFE-DDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELP 335
              K+ L++ Q K +   E   ++D +D+DDD LSC+SSDLFELDNLS IGI+RYREELP
Sbjct: 312 EAAKELLRSYQKKNKEVIEVSVEDDEEDDDDDALSCTSSDLFELDNLSAIGIDRYREELP 365

BLAST of MC08g0855 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.3e-49
Identity = 162/367 (44.14%), Postives = 207/367 (56.40%), Query Frame = 0

Query: 4   WDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQ 63
           W+KP     ++  H R     NPSFSS+LLD IYRSID+S+     P L   ++     Q
Sbjct: 5   WEKP-----KSSSHHR-----NPSFSSTLLDQIYRSIDDSS---PPPPLESIKKKKHHHQ 64

Query: 64  SIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSS------SSSESSCTAGRF 123
              A+  ED + +  + R     + ++ +  ++    +NSSS      SSSES    GR 
Sbjct: 65  QRNASLHEDREISPIYHRRSIAADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRS 124

Query: 124 SSSESEFLS---RPKPIKTTT---EIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISP 183
            SS S   S   +PKPI+T++        KPK   GFL+TKSKA K+Y DLKKVKQPISP
Sbjct: 125 KSSASPPSSSRQQPKPIRTSSVDHSSAVQKPKELGGFLRTKSKALKIYSDLKKVKQPISP 184

Query: 184 GARLANFLNSLF-NGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGN 243
           G RLA FLNSLF N  T   K K + +     +    STCSS SSFSRSCLSKTPSS G 
Sbjct: 185 GGRLATFLNSLFTNAATNPKKHKKTTTVAVVEEPHSSSTCSSASSFSRSCLSKTPSSSGK 244

Query: 244 AAKRSVRFCPVSVIVDEDC-----------RPCGHKILHKIE------PILKKPLKTEQV 303
            +KRSVRFCPV+VI+DED            R   +    ++E         K  L+T   
Sbjct: 245 -SKRSVRFCPVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLRTYHN 304

Query: 304 KTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTNF-KTNCA 340
           K +    + +   +D++DD  SC+SSDLFEL+NLS IGIERYREELPVYETT     N  
Sbjct: 305 KNKVTTTNINNVEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRV 357

BLAST of MC08g0855 vs. ExPASy Swiss-Prot
Match: Q9M2B3 (Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.9e-28
Identity = 121/344 (35.17%), Postives = 178/344 (51.74%), Query Frame = 0

Query: 22  RRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALK--F 81
           R+  PSFSSS+LD++YRSIDES+G   + +  +    + +  S +  + +D    L+   
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSDLKGSINENVSSSSSSPSPNKKDDKLTTLRRAI 65

Query: 82  RRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFS--SSESEFLSRPKPIKTTTEI 141
              E W+  +     +N  D ++ SSS +ES  T  R    + + +     +     T +
Sbjct: 66  MDEEHWLYARSSTTTTNSSDSSSFSSSEAESYRTKRRLRKLAEQGKRSGDERQRTKRTVM 125

Query: 142 PKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKLSNS 201
                         K KA K+  +LK+ KQP+SPGARL +FLNS+F     K K      
Sbjct: 126 DNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNAKKVKL----C 185

Query: 202 SVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAK--RSVRFCPVSVIVDEDCRPCGHK 261
           SV  +  ++ S  SS S FSR+  +KT ++  N  K  RS+RF PV V +D DCR    K
Sbjct: 186 SVGKTTDVKSS--SSKSCFSRT-RNKTDNNNNNCKKLERSIRFYPVRVTIDGDCRDYAQK 245

Query: 262 ILHKI-EPI--------LKKPLKTEQVKTE----------HDF-EDDDEDYDDEDDDGLS 321
            + ++ +PI        +K+ +KT    TE           DF   +  +  +E++D  S
Sbjct: 246 HITRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITRNIGLKDFVRSNKYEGKEEEEDAWS 305

Query: 322 CSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 340
            SSSDLFELD+   IG+ RY +ELPVYETT+FKTN AIA  L+L
Sbjct: 306 HSSSDLFELDSYR-IGMGRYLKELPVYETTDFKTNQAIARSLLL 341

BLAST of MC08g0855 vs. ExPASy Swiss-Prot
Match: Q10R09 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.5e-27
Identity = 123/331 (37.16%), Postives = 171/331 (51.66%), Query Frame = 0

Query: 19  RRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALK 78
           RR   + PSFSS+LLDAIY+S+DE  G G     +         +++AAA  +  + AL 
Sbjct: 15  RRYVADQPSFSSTLLDAIYKSMDEQPGHGGGATGV---------EAVAAAAKKQHEAALH 74

Query: 79  FRRVEKWMEMKKKKVGS-NVRDFTNSSSSSSESSCTA-GRFSSSESE--FLSRPKPIKTT 138
           +         K    GS   R     +++SS S C++ G FSSSE+E     R +PI+TT
Sbjct: 75  YGNY-----YKPSLAGSYRARAPGPHATTSSSSECSSYGGFSSSEAESSHHRRLRPIRTT 134

Query: 139 TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKL 198
                P P  E    K K   + +   L+ +++P SPGARLA FLNS+F G       K 
Sbjct: 135 VPGGAPGPAPEK---KAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAG-------KR 194

Query: 199 SNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRF-------CPVSVIVDE 258
           + ++  S+ +   S CS+ SS+SRSCLSKTPS+RG  AKR+VRF          S +VD 
Sbjct: 195 APATPPSATAGAESACSTASSYSRSCLSKTPSTRGQ-AKRTVRFLDSDTESLASSTVVDR 254

Query: 259 DCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNL 318
              P     +  ++ +L + ++ E                DEDDD  S +SSDLFEL+N 
Sbjct: 255 RRVP-----VEAVQQMLLQRMEME---------------SDEDDDESSDASSDLFELENF 300

Query: 319 SVIGI--ERYREELPVYETTNFKTNCAIANG 337
           + I      YR+ELPVYETT    N AI +G
Sbjct: 315 AAIAPAGAAYRDELPVYETTRVALNRAIGHG 300

BLAST of MC08g0855 vs. ExPASy Swiss-Prot
Match: B8APC7 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. indica OX=39946 GN=BG1 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.2e-27
Identity = 123/331 (37.16%), Postives = 171/331 (51.66%), Query Frame = 0

Query: 19  RRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALK 78
           RR   + PSFSS+LLDAIY+S+DE  G G     +         +++AAA  +  + AL 
Sbjct: 15  RRYVADQPSFSSTLLDAIYKSMDEQPGHGGGATGV---------EAVAAAAKKQHEAALH 74

Query: 79  FRRVEKWMEMKKKKVGS-NVRDFTNSSSSSSESSCTA-GRFSSSESE--FLSRPKPIKTT 138
           +         K    GS   R     +++SS S C++ G FSSSE+E     R +PI+TT
Sbjct: 75  Y-----GYYYKPSLAGSYRARAPGPHATTSSSSECSSYGGFSSSEAESSHHRRLRPIRTT 134

Query: 139 TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKL 198
                P P  E    K K   + +   L+ +++P SPGARLA FLNS+F G       K 
Sbjct: 135 VPGGAPGPAPEK---KAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAG-------KR 194

Query: 199 SNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRF-------CPVSVIVDE 258
           + ++  S+ +   S CS+ SS+SRSCLSKTPS+RG  AKR+VRF          S +VD 
Sbjct: 195 APATPPSATAGAESACSTASSYSRSCLSKTPSTRGQ-AKRTVRFLDSDTESLASSTVVDR 254

Query: 259 DCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNL 318
              P     +  ++ +L + ++ E                DEDDD  S +SSDLFEL+N 
Sbjct: 255 RRVP-----VEAVQQMLLQRMEME---------------SDEDDDESSDASSDLFELENF 300

Query: 319 SVIGI--ERYREELPVYETTNFKTNCAIANG 337
           + I      YR+ELPVYETT    N AI +G
Sbjct: 315 AAIAPAGAAYRDELPVYETTRVALNRAIGHG 300

BLAST of MC08g0855 vs. NCBI nr
Match: XP_022152666.1 (protein BIG GRAIN 1-like B [Momordica charantia])

HSP 1 Score: 649 bits (1673), Expect = 3.00e-234
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 60
           FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM
Sbjct: 3   FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 62

Query: 61  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS 120
           TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS
Sbjct: 63  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS 122

Query: 121 ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN 180
           ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN
Sbjct: 123 ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN 182

Query: 181 SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP 240
           SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP
Sbjct: 183 SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP 242

Query: 241 VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD 300
           VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD
Sbjct: 243 VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD 302

Query: 301 LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 339
           LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL
Sbjct: 303 LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 341

BLAST of MC08g0855 vs. NCBI nr
Match: XP_038903204.1 (protein BIG GRAIN 1-like B [Benincasa hispida])

HSP 1 Score: 407 bits (1046), Expect = 2.48e-138
Identity = 256/362 (70.72%), Postives = 286/362 (79.01%), Query Frame = 0

Query: 18  RRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM-TKQSIA-AARTEDADE 77
           RRR RRENPSFSSSLLDAIYRSIDESN Q E+  ++  ++TT+ TKQSI+ AARTED DE
Sbjct: 17  RRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLILYTQKTTLRTKQSISSAARTED-DE 76

Query: 78  ALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGR-FSSSESEFLSR---- 137
            LKF R   ++KWM+ KK +   N+RDF+ SSSSSSESS TAGR FSSSESEFLSR    
Sbjct: 77  TLKFHRACIIDKWMDKKKLR---NIRDFSLSSSSSSESSSTAGRRFSSSESEFLSRALHR 136

Query: 138 ----PKPIKTTT----EIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFL 197
               PKPIKTTT    EI  PKPKHENGF+K+KSKASK+YHDLKKVKQPISPG RLA+FL
Sbjct: 137 PKPKPKPIKTTTWAQTEIISPKPKHENGFVKSKSKASKIYHDLKKVKQPISPGGRLASFL 196

Query: 198 NSLFNGGTPKTKQKLSNSSV-----------QSSKSLQ--GSTCSSTSSFSRSCLSKTPS 257
           NSLFNGG+PKTKQK+SNSS            + SKS Q   STCSS SSFSRSCLSKTPS
Sbjct: 197 NSLFNGGSPKTKQKISNSSSINSTNFDYDMSRKSKSQQVSTSTCSSASSFSRSCLSKTPS 256

Query: 258 SRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKIE-PILKKP-------LKTEQVKTEHD 317
           SRGN  KRSVRFCPVSVIVDEDC+PCG KILHK+E PI+KK         +TE+VKTEH+
Sbjct: 257 SRGNI-KRSVRFCPVSVIVDEDCQPCGRKILHKLEEPIMKKGNLKSYRNFETEKVKTEHE 316

Query: 318 FEDDDE-DYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIANGL 339
            + + E D DD+DDD LSCSSSDLFELDNLSVIGIERYREELPVYETT+F TNCAIANGL
Sbjct: 317 MKINYELDEDDDDDDALSCSSSDLFELDNLSVIGIERYREELPVYETTHFNTNCAIANGL 373

BLAST of MC08g0855 vs. NCBI nr
Match: XP_022948938.1 (protein BIG GRAIN 1-like B [Cucurbita moschata])

HSP 1 Score: 404 bits (1039), Expect = 2.50e-137
Identity = 253/376 (67.29%), Postives = 282/376 (75.00%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARD--HRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRET 60
            +LWDK    QR  +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRET
Sbjct: 1   MDLWDKS--FQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRET 60

Query: 61  TM-TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTA 120
           TM TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TA
Sbjct: 61  TMRTKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTA 120

Query: 121 GR-FSSSESEFLSRP----KPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQ 180
           GR +S+SESE LSR     KPIKTT  T+    K KHENG +KTKSKASK+YHDLKKVKQ
Sbjct: 121 GRRYSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQ 180

Query: 181 PISPGARLANFLNSLFNGGTPKTKQKLSNSSVQ---------SSKSLQGSTCSSTSSFSR 240
           PISPG RLANFLNSLFNGG+PKTKQK+ N S           +    QGSTCSS SSFSR
Sbjct: 181 PISPGGRLANFLNSLFNGGSPKTKQKMPNPSTNPRSMDYDMPTKAKSQGSTCSSASSFSR 240

Query: 241 SCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKIE-PILKKPLK------TE 300
           SCLSKTPSSRGN  KRSVRFCPVS IVDEDC   GHK L K+E PI+KK LK      TE
Sbjct: 241 SCLSKTPSSRGNV-KRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGKFETE 300

Query: 301 QVKTEHD--------FEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYE 339
           +VKTEH         FE +DED ++E+DD +SCSSSDLFELDNLSVIG+ERYREELPVYE
Sbjct: 301 EVKTEHPRKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYE 360

BLAST of MC08g0855 vs. NCBI nr
Match: XP_023525389.1 (protein BIG GRAIN 1-like B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 403 bits (1036), Expect = 7.13e-137
Identity = 252/376 (67.02%), Postives = 281/376 (74.73%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARD--HRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRET 60
            +LWDK    QR  +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRET
Sbjct: 1   MDLWDKS--FQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRET 60

Query: 61  TM-TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTA 120
           TM TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TA
Sbjct: 61  TMRTKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTA 120

Query: 121 GR-FSSSESEFLSR----PKPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQ 180
           GR +S+SESE LSR    PKPIKTT  T+    K KHENG +KTKSKASKMYHDLKKVKQ
Sbjct: 121 GRRYSASESELLSRALHRPKPIKTTPWTQTETQKAKHENGLVKTKSKASKMYHDLKKVKQ 180

Query: 181 PISPGARLANFLNSLFNGGTPKTKQKLSNSSVQ---------SSKSLQGSTCSSTSSFSR 240
           PISPG RLANFLNSLFNGG+PKTKQK  N S           +    QGSTCSS SSFSR
Sbjct: 181 PISPGGRLANFLNSLFNGGSPKTKQKTPNRSTNPRSMDYDMPTKAKSQGSTCSSASSFSR 240

Query: 241 SCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKIE-PILKKPLK------TE 300
           SCLSKTP SRGN  KRSVRFCPVS IVDEDC   GHK L K+E PI+KK LK      TE
Sbjct: 241 SCLSKTPCSRGNV-KRSVRFCPVSEIVDEDCEADGHKSLQKLEEPIMKKGLKSYGKFETE 300

Query: 301 QVKTEHD--------FEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYE 339
           +VKTEH         F+ +DED ++E+DD +SCSSSDLFELDNLSVIG+ERYREELPVYE
Sbjct: 301 EVKTEHPRKSKGNEGFDYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYE 360

BLAST of MC08g0855 vs. NCBI nr
Match: KAG6606698.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 398 bits (1022), Expect = 9.51e-135
Identity = 251/376 (66.76%), Postives = 280/376 (74.47%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARD--HRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRET 60
            +LWDK    QR  +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRET
Sbjct: 1   MDLWDKS--FQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRET 60

Query: 61  TM-TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTA 120
           TM TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TA
Sbjct: 61  TMRTKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTA 120

Query: 121 GR-FSSSESEFLSRP----KPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQ 180
           GR +S+SESE LSR     KPIKTT  T+    K KHENG +KTKSKASK+YHDLKKVKQ
Sbjct: 121 GRRYSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKVYHDLKKVKQ 180

Query: 181 PISPGARLANFLNSLFNGGTPKTKQKLSNSSVQ---------SSKSLQGSTCSSTSSFSR 240
           PISPG RLANFLNSLFNGG+PKTKQK+ N S           +    QGSTCSS SSFSR
Sbjct: 181 PISPGGRLANFLNSLFNGGSPKTKQKIPNPSTNPRSMDYDMATKAKSQGSTCSSASSFSR 240

Query: 241 SCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKIE-PILKKPLK------TE 300
           SCLSKTPSSRGN  KRSVRFCPVS IV+EDC   G K L K+E PI+KK LK      TE
Sbjct: 241 SCLSKTPSSRGNV-KRSVRFCPVSEIVEEDCEAYGQKSLQKLEEPIMKKGLKSYGKFETE 300

Query: 301 QVKTEHD--------FEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYE 339
           +VKTEH         FE +DED ++E+DD  SCSSSDLFELDNLSVIG+ERYREELPVYE
Sbjct: 301 EVKTEHPRKSKGNEGFEYEDEDEEEEEDDAGSCSSSDLFELDNLSVIGMERYREELPVYE 360

BLAST of MC08g0855 vs. ExPASy TrEMBL
Match: A0A6J1DIF0 (protein BIG GRAIN 1-like B OS=Momordica charantia OX=3673 GN=LOC111020328 PE=3 SV=1)

HSP 1 Score: 649 bits (1673), Expect = 1.45e-234
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 60
           FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM
Sbjct: 3   FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 62

Query: 61  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS 120
           TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS
Sbjct: 63  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS 122

Query: 121 ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN 180
           ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN
Sbjct: 123 ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN 182

Query: 181 SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP 240
           SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP
Sbjct: 183 SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP 242

Query: 241 VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD 300
           VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD
Sbjct: 243 VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD 302

Query: 301 LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 339
           LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL
Sbjct: 303 LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 341

BLAST of MC08g0855 vs. ExPASy TrEMBL
Match: A0A6J1GAP7 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV=1)

HSP 1 Score: 404 bits (1039), Expect = 1.21e-137
Identity = 253/376 (67.29%), Postives = 282/376 (75.00%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARD--HRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRET 60
            +LWDK    QR  +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRET
Sbjct: 1   MDLWDKS--FQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRET 60

Query: 61  TM-TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTA 120
           TM TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TA
Sbjct: 61  TMRTKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTA 120

Query: 121 GR-FSSSESEFLSRP----KPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQ 180
           GR +S+SESE LSR     KPIKTT  T+    K KHENG +KTKSKASK+YHDLKKVKQ
Sbjct: 121 GRRYSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQ 180

Query: 181 PISPGARLANFLNSLFNGGTPKTKQKLSNSSVQ---------SSKSLQGSTCSSTSSFSR 240
           PISPG RLANFLNSLFNGG+PKTKQK+ N S           +    QGSTCSS SSFSR
Sbjct: 181 PISPGGRLANFLNSLFNGGSPKTKQKMPNPSTNPRSMDYDMPTKAKSQGSTCSSASSFSR 240

Query: 241 SCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKIE-PILKKPLK------TE 300
           SCLSKTPSSRGN  KRSVRFCPVS IVDEDC   GHK L K+E PI+KK LK      TE
Sbjct: 241 SCLSKTPSSRGNV-KRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGKFETE 300

Query: 301 QVKTEHD--------FEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYE 339
           +VKTEH         FE +DED ++E+DD +SCSSSDLFELDNLSVIG+ERYREELPVYE
Sbjct: 301 EVKTEHPRKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYE 360

BLAST of MC08g0855 vs. ExPASy TrEMBL
Match: A0A1S3CKK1 (protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1)

HSP 1 Score: 392 bits (1006), Expect = 1.56e-132
Identity = 247/384 (64.32%), Postives = 280/384 (72.92%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 60
            +LWD   P         RR RR+NPSFSSSLLDAIYRSIDESN Q E+  LI Y   T 
Sbjct: 1   MDLWDHSDP---HLLQQPRRSRRDNPSFSSSLLDAIYRSIDESNSQPED-HLIFYTHNT- 60

Query: 61  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGR-FSS 120
           TK +I  A      E+LKFR ++ W++ KKK    N+RDFT SSSSSSESS TAGR FSS
Sbjct: 61  TKHTILPATVSQDHESLKFRMIDTWIDKKKKLR--NIRDFTLSSSSSSESSSTAGRRFSS 120

Query: 121 SESEFLSR-----------PKPIKTTT----EIPKPKPKHENGFLKTKSKASKMYHDLKK 180
           SE+EFLSR           PKPIKT T    EI  P PKH+NGF+K+KSKASK+YHDLKK
Sbjct: 121 SETEFLSRALHRPTKLKPKPKPIKTNTWPQTEIITPNPKHDNGFVKSKSKASKIYHDLKK 180

Query: 181 VKQPISPGARLANFLNSLFNGGTPKTKQKLSNSSV------------QSSKSLQGST--C 240
           VKQPISPGARLA+FLNSLFNGG+PK KQK+S+S+             + SKS QGST  C
Sbjct: 181 VKQPISPGARLASFLNSLFNGGSPKAKQKISSSTCSINSTKFDYDMSRKSKSQQGSTSTC 240

Query: 241 SSTSSFSRSCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKIE-PILKKP-- 300
           SS SSFSRSCLSKTPSSRGN  KRSVRFCPVSVIVDEDC+PCGHKILHK E PI+KK   
Sbjct: 241 SSASSFSRSCLSKTPSSRGNI-KRSVRFCPVSVIVDEDCQPCGHKILHKSEEPIMKKGNL 300

Query: 301 ------LKTEQVKTE------HDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERY 339
                 L+ E+VKTE      ++ E++++D DD+DDD LSCSSSDLFELDNLSVIGIERY
Sbjct: 301 KKSNGNLEREKVKTEDMMKINYEEEEEEDDDDDDDDDALSCSSSDLFELDNLSVIGIERY 360

BLAST of MC08g0855 vs. ExPASy TrEMBL
Match: A0A6J1KB46 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 1.99e-132
Identity = 251/376 (66.76%), Postives = 278/376 (73.94%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARD--HRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRET 60
            +LWDK    QR  +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRET
Sbjct: 84  MDLWDKS--FQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRET 143

Query: 61  TM-TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTA 120
           TM TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TA
Sbjct: 144 TMRTKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTA 203

Query: 121 GR-FSSSESEFLSRP----KPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQ 180
           GR +S+SESE LSR     KPIKTT  T+    K KHENG +KTKSKASK+YHDLKKVKQ
Sbjct: 204 GRRYSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQ 263

Query: 181 PISPGARLANFLNSLFNGGTPKTKQKLSNSSVQSSK---------SLQGSTCSSTSSFSR 240
           PISPG RLANFLNSLFNGG+PKTKQK+ N S   +            QGSTCSS SSFSR
Sbjct: 264 PISPGGRLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSR 323

Query: 241 SCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKIE-PILKKPLK------TE 300
           SCLSKTPSSRGN  KRSVRFCPVS IVDEDC   GHK L K+E PI+KK LK      TE
Sbjct: 324 SCLSKTPSSRGNV-KRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETE 383

Query: 301 QVKTEHD--------FEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYE 339
           +VKTEH         FE     Y+DEDD  +SCSSSDLFELDNLSVIGIER+REELPVYE
Sbjct: 384 EVKTEHPRKIKRNEGFE-----YEDEDD-AVSCSSSDLFELDNLSVIGIERFREELPVYE 443

BLAST of MC08g0855 vs. ExPASy TrEMBL
Match: A0A6J1IZW5 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1)

HSP 1 Score: 387 bits (994), Expect = 6.65e-131
Identity = 251/381 (65.88%), Postives = 282/381 (74.02%), Query Frame = 0

Query: 1   FNLWDKPQPLQREARD-----HRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILY 60
            +LWD     QRE  D      RRR RRENPSFSSSLLDAIYRSID+SN QGE+ QLI Y
Sbjct: 1   MDLWDNS--FQREDPDPHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNVQGED-QLIFY 60

Query: 61  RE--TTMTKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSES 120
            +  TT TKQSI+AA+TED  + L+F+R   ++ WM+ KK K    VRDFT S+SSSSES
Sbjct: 61  TQKTTTRTKQSISAAKTED-HQTLQFQRASMIKNWMDNKKIKT---VRDFTVSTSSSSES 120

Query: 121 SCTAGR-FSSSESEFLSR------------PKPIKTTT----EIPKPKPKHENGFLKTKS 180
           S TAGR  SSSESEFLSR            PKPIKTTT    EI  P P HENGF+K+K 
Sbjct: 121 SSTAGRRLSSSESEFLSRTLHRPKPKPKPKPKPIKTTTWSQTEII-PNPNHENGFVKSKF 180

Query: 181 KASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKLSNS-----------SVQSS 240
           +ASK+YHDLKKVKQPISPG RLANFLNSLFNGG+PK KQK+SN+           + + S
Sbjct: 181 RASKIYHDLKKVKQPISPGGRLANFLNSLFNGGSPKAKQKISNTCSNNSTNLDYDAPRKS 240

Query: 241 KSLQGS--TCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI 300
           KS QGS  TCSS SSFSRSCL KTPSSRGN  KRSVRFCPVSVIVDEDCRPCGHK+LHK+
Sbjct: 241 KSQQGSSSTCSSASSFSRSCLIKTPSSRGNI-KRSVRFCPVSVIVDEDCRPCGHKMLHKL 300

Query: 301 E-PILKK-PLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREE 339
           E PI KK  L+TE++KTE D         DEDDD LSCSSSDLFELDNLS IG+ERYR+E
Sbjct: 301 EEPIGKKGKLETEEMKTEED---------DEDDDRLSCSSSDLFELDNLSAIGMERYRDE 360

BLAST of MC08g0855 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 209.9 bits (533), Expect = 3.2e-54
Identity = 165/374 (44.12%), Postives = 210/374 (56.15%), Query Frame = 0

Query: 16  DHRRRLR----RENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTE 75
           DH+ RLR      +PSFSS+LLD IYRSID+S+      +   ++    T+  ++A R +
Sbjct: 12  DHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRKTKHQNREDTR--VSANRRD 71

Query: 76  DADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSSESEF------- 135
           D + +   + +E                F +SSSSSS+SS     FSSSES++       
Sbjct: 72  DFNRSKNLKTIEPVF-------------FKHSSSSSSDSS----GFSSSESDYFYRRSKS 131

Query: 136 ---LSRPKPIKTTTE---------IPKPKPKHENG-FLKTKSKASKMYHDLKKVKQPISP 195
              +S PKPI+TT E          P    K E+G FLKTKSKA K+Y DLKKVKQPISP
Sbjct: 132 SPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKALKIYSDLKKVKQPISP 191

Query: 196 GARLANFLNSLFNGGTPKTKQKLSNSSVQSSKSLQG-----STCSSTSSFSRSCLSKTPS 255
           G RLA FLNS+F G     K    N++V S+ +        +TCSS SSFSRSCLSKTPS
Sbjct: 192 GGRLATFLNSIFTGAGNTKKLNKINTTVTSTTAAAAAASSTTTCSSASSFSRSCLSKTPS 251

Query: 256 SRGNAAKRSVRFCPVSVIVDEDCRPCGHK--------------ILHKIE----------- 315
           S    +KRSVRFCPV+VI DED     +K              I H +E           
Sbjct: 252 S-SEKSKRSVRFCPVNVIFDEDSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVI 311

Query: 316 PILKKPLKTEQVKTEHDFE-DDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELP 335
              K+ L++ Q K +   E   ++D +D+DDD LSC+SSDLFELDNLS IGI+RYREELP
Sbjct: 312 EAAKELLRSYQKKNKEVIEVSVEDDEEDDDDDALSCTSSDLFELDNLSAIGIDRYREELP 365

BLAST of MC08g0855 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-50
Identity = 162/367 (44.14%), Postives = 207/367 (56.40%), Query Frame = 0

Query: 4   WDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQ 63
           W+KP     ++  H R     NPSFSS+LLD IYRSID+S+     P L   ++     Q
Sbjct: 5   WEKP-----KSSSHHR-----NPSFSSTLLDQIYRSIDDSS---PPPPLESIKKKKHHHQ 64

Query: 64  SIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSS------SSSESSCTAGRF 123
              A+  ED + +  + R     + ++ +  ++    +NSSS      SSSES    GR 
Sbjct: 65  QRNASLHEDREISPIYHRRSIAADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRS 124

Query: 124 SSSESEFLS---RPKPIKTTT---EIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISP 183
            SS S   S   +PKPI+T++        KPK   GFL+TKSKA K+Y DLKKVKQPISP
Sbjct: 125 KSSASPPSSSRQQPKPIRTSSVDHSSAVQKPKELGGFLRTKSKALKIYSDLKKVKQPISP 184

Query: 184 GARLANFLNSLF-NGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGN 243
           G RLA FLNSLF N  T   K K + +     +    STCSS SSFSRSCLSKTPSS G 
Sbjct: 185 GGRLATFLNSLFTNAATNPKKHKKTTTVAVVEEPHSSSTCSSASSFSRSCLSKTPSSSGK 244

Query: 244 AAKRSVRFCPVSVIVDEDC-----------RPCGHKILHKIE------PILKKPLKTEQV 303
            +KRSVRFCPV+VI+DED            R   +    ++E         K  L+T   
Sbjct: 245 -SKRSVRFCPVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLRTYHN 304

Query: 304 KTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTNF-KTNCA 340
           K +    + +   +D++DD  SC+SSDLFEL+NLS IGIERYREELPVYETT     N  
Sbjct: 305 KNKVTTTNINNVEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRV 357

BLAST of MC08g0855 vs. TAIR 10
Match: AT3G42800.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 853 Blast hits to 340 proteins in 98 species: Archae - 0; Bacteria - 94; Metazoa - 145; Fungi - 62; Plants - 95; Viruses - 0; Other Eukaryotes - 457 (source: NCBI BLink). )

HSP 1 Score: 126.7 bits (317), Expect = 3.5e-29
Identity = 121/344 (35.17%), Postives = 178/344 (51.74%), Query Frame = 0

Query: 22  RRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALK--F 81
           R+  PSFSSS+LD++YRSIDES+G   + +  +    + +  S +  + +D    L+   
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSDLKGSINENVSSSSSSPSPNKKDDKLTTLRRAI 65

Query: 82  RRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFS--SSESEFLSRPKPIKTTTEI 141
              E W+  +     +N  D ++ SSS +ES  T  R    + + +     +     T +
Sbjct: 66  MDEEHWLYARSSTTTTNSSDSSSFSSSEAESYRTKRRLRKLAEQGKRSGDERQRTKRTVM 125

Query: 142 PKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKLSNS 201
                         K KA K+  +LK+ KQP+SPGARL +FLNS+F     K K      
Sbjct: 126 DNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNAKKVKL----C 185

Query: 202 SVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAK--RSVRFCPVSVIVDEDCRPCGHK 261
           SV  +  ++ S  SS S FSR+  +KT ++  N  K  RS+RF PV V +D DCR    K
Sbjct: 186 SVGKTTDVKSS--SSKSCFSRT-RNKTDNNNNNCKKLERSIRFYPVRVTIDGDCRDYAQK 245

Query: 262 ILHKI-EPI--------LKKPLKTEQVKTE----------HDF-EDDDEDYDDEDDDGLS 321
            + ++ +PI        +K+ +KT    TE           DF   +  +  +E++D  S
Sbjct: 246 HITRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITRNIGLKDFVRSNKYEGKEEEEDAWS 305

Query: 322 CSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 340
            SSSDLFELD+   IG+ RY +ELPVYETT+FKTN AIA  L+L
Sbjct: 306 HSSSDLFELDSYR-IGMGRYLKELPVYETTDFKTNQAIARSLLL 341

BLAST of MC08g0855 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 100.5 bits (249), Expect = 2.7e-21
Identity = 129/375 (34.40%), Postives = 176/375 (46.93%), Query Frame = 0

Query: 23  RENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALKFRRV 82
           + NPSFSS+LLD IY SID    Q  +P  +    TT  KQSI      D     K  R 
Sbjct: 5   KRNPSFSSTLLDEIYNSIDPKT-QKTQP-YVGSVNTTTKKQSIVTRSVPDR----KIHRD 64

Query: 83  EKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSSESEFL---SRPKPIKT------ 142
             +  +      S+  D  +S  SSS++  T G+ ++S        S+ KP KT      
Sbjct: 65  RFFGSV------SSSSDSNSSIFSSSDTELTHGKKTTSSRPLCFGPSKTKPRKTEDKTLF 124

Query: 143 --------------TTEIPKPKPKHENGFLKTKSKAS-KMYHDLKKVKQPISPGARLANF 202
                          +++PK   +H+  +  T+++ S K   + KK K P SPG R+ NF
Sbjct: 125 HQNRATRVYDDYDYASDVPKFN-RHDENWENTRNRRSVKSSGNQKKPKTPASPGGRIVNF 184

Query: 203 LNSLFNGGTPKTK-----------------QKLSNSSVQSSKSLQGSTCSSTSSFSRSCL 262
           LNSLF+  + ++                  +K SN    S+     +TCSS SSFSRSC+
Sbjct: 185 LNSLFSNNSKQSNAVKSYPRKTSYDDSAYVRKTSNDYHSST-----TTCSSASSFSRSCM 244

Query: 263 SKTPSSRGNAAKRSVRFCPVSVIV-------DEDCRPCGH--KILHK---------IEPI 322
           +K         KRSVRF PV+VIV       +ED    G+  K + K         +E I
Sbjct: 245 NKGYEKSSGRIKRSVRFSPVNVIVPESFTSKEEDYFSNGNARKSVKKNVEDGGRRSVEEI 304

Query: 323 LKKPLK-------TEQVKTE--HDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIER 326
            ++ L+          VKT    D+EDDDE  DD+DDD  S SSSDLFELD   ++G   
Sbjct: 305 AREFLRDYHKNHENSLVKTNGFEDYEDDDE--DDDDDDVASDSSSDLFELD---LVGNHH 356

BLAST of MC08g0855 vs. TAIR 10
Match: AT1G69160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 173 Blast hits to 172 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 25; Fungi - 33; Plants - 84; Viruses - 2; Other Eukaryotes - 29 (source: NCBI BLink). )

HSP 1 Score: 42.7 bits (99), Expect = 6.7e-04
Identity = 66/209 (31.58%), Postives = 91/209 (43.54%), Query Frame = 0

Query: 166 KQPISPGARLANFLNSLFN-GGTPKTKQKLSNSS-------------------------- 225
           KQP SPG ++A+FLNSLF+  G+ K K K  + +                          
Sbjct: 114 KQPSSPGGKIASFLNSLFHQAGSKKNKSKSKSKTKPTDPEVEEEIPGGGWMRRRRRSSIS 173

Query: 226 --VQSSKSLQGSTCSSTSSFSRSCLS------KTPSSRGNAA----KRSVRFCPVSVIVD 285
               SS+S   +T ++ SS S+S +S      +TP    N      K+ + +   +  V 
Sbjct: 174 HFFSSSRSTSTTTTTTASSSSKSLISSSSSGFRTPPPYLNTPTKNYKQFLNYTSATKQVG 233

Query: 286 EDCRPCGHKILHKIEPILKKPLKT-EQVKTEHDFEDDDEDYDDE-------DDDGL-SCS 327
           E+         +K    L + LK  E +        DDED DD+       +DDG+ S S
Sbjct: 234 EE-----ETKTNKEYSWLDEKLKVMESLSENQRIWSDDEDIDDDRRIKREGEDDGMESDS 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SLL24.4e-5344.12Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=... [more]
Q9LVK22.3e-4944.14Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=... [more]
Q9M2B34.9e-2835.17Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=... [more]
Q10R092.5e-2737.16Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1[more]
B8APC73.2e-2737.16Protein BIG GRAIN 1 OS=Oryza sativa subsp. indica OX=39946 GN=BG1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022152666.13.00e-234100.00protein BIG GRAIN 1-like B [Momordica charantia][more]
XP_038903204.12.48e-13870.72protein BIG GRAIN 1-like B [Benincasa hispida][more]
XP_022948938.12.50e-13767.29protein BIG GRAIN 1-like B [Cucurbita moschata][more]
XP_023525389.17.13e-13767.02protein BIG GRAIN 1-like B [Cucurbita pepo subsp. pepo][more]
KAG6606698.19.51e-13566.76Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1DIF01.45e-234100.00protein BIG GRAIN 1-like B OS=Momordica charantia OX=3673 GN=LOC111020328 PE=3 S... [more]
A0A6J1GAP71.21e-13767.29protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV... [more]
A0A1S3CKK11.56e-13264.32protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1[more]
A0A6J1KB461.99e-13266.76protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1[more]
A0A6J1IZW56.65e-13165.88protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G54200.13.2e-5444.12unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G13980.11.6e-5044.14unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G42800.13.5e-2935.17unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G12050.12.7e-2134.40unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G69160.16.7e-0431.58unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 271..290
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..230
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..151
NoneNo IPR availablePANTHERPTHR33541:SF2PROTEIN BIG GRAIN 1-LIKE Acoord: 15..339
IPR039621Protein BIG GRAIN 1-likePANTHERPTHR33541PROTEIN BIG GRAIN 1-LIKE A-RELATEDcoord: 15..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0855.1MC08g0855.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0005886 plasma membrane