MC08g0843 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g0843 vs. ExPASy Swiss-Prot
Match: B6SFA4 (Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 5.8e-272
Identity = 499/830 (60.12%), Postives = 625/830 (75.30%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTS--RLFKIILSWDYFRLLKDSKKRKDDDGSSLGLKEVKASYKDVDD 60
           MA+D  K+ EE  +S  R + IIL WDY +L K++ +RK+   S   L  VK +YKDVDD
Sbjct: 1   MAIDNGKLQEEEASSVTRFYNIILGWDYKQLTKEN-ERKNRKDSKEKLNVVKNTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           Y  TFEPLL EEVKAQI+Q  D EEA+  K R +MECNE  GFHF  + Y  EE+     
Sbjct: 61  YFETFEPLLFEEVKAQILQNKDGEEASVCKMRLVMECNEGEGFHFLLVTYEHEED----- 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
               E+L+ NDLLLLSKE+ + N+  P++Y FA+VE RQ + LRLRMYLA ++     + 
Sbjct: 121 ----EYLAQNDLLLLSKEEVKGNS-FPSSYGFAVVEHRQNNLLRLRMYLAEDIVQITKNT 180

Query: 181 IVSSPR-----LLKVRSHITSS-SKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMI 240
             S  +     L  +RS ITSS S     ++SLK+C LSTIIREYIAL S+SSLPFKD+I
Sbjct: 181 KSSRTKSFIQALSNMRSLITSSASPIDKRVFSLKLCGLSTIIREYIALRSVSSLPFKDLI 240

Query: 241 LAAADKNTS-KDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQT 300
             AA+K+    D+AWKIS PL ++  ENLN+SQ+ A+  GLSRK FVLIQGPPGTGKTQT
Sbjct: 241 FTAAEKSCGFGDEAWKISGPLNEFFNENLNKSQKEAIDVGLSRKSFVLIQGPPGTGKTQT 300

Query: 301 ILGLLSAILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVN 360
           IL +L AI+HATPAR+ SK    E K+G ++ ++EKY+HW +ASPW+ G+NPRD IMP +
Sbjct: 301 ILSILGAIMHATPARVQSKGTDHEVKRGIQMTIQEKYNHWGRASPWILGVNPRDAIMPED 360

Query: 361 GDDGFFPTSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTP 420
           GDDGFFPTSGNELKPEVV ++RKYR+RVLVCAPSNSALDEIVLR+ ++G+RDEN   YTP
Sbjct: 361 GDDGFFPTSGNELKPEVVNASRKYRLRVLVCAPSNSALDEIVLRLLSSGLRDENAQTYTP 420

Query: 421 KIVRIGLKAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFST 480
           KIVRIGLKAH S+ +VS+  LV QK+ S    K K G +GTD+DSIR+AIL+E+ IVF+T
Sbjct: 421 KIVRIGLKAHHSVASVSLDHLVAQKRGS-AIDKPKQGTTGTDIDSIRTAILEEAAIVFAT 480

Query: 481 LSFSGSSLFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTT 540
           LSFSGS+L +K NRGFDVVIIDEAAQAVE ATL+PLA  CKQVFLVGDP+QLPATVIST 
Sbjct: 481 LSFSGSALLAKSNRGFDVVIIDEAAQAVEPATLIPLATRCKQVFLVGDPKQLPATVISTV 540

Query: 541 AKKFGYDKSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRA 600
           A+  GY  S+F+R Q AGYPV MLK QYRMHPEIRSFPS++FY  +LED  D++ +TTR 
Sbjct: 541 AQDSGYGTSMFERLQKAGYPVKMLKTQYRMHPEIRSFPSKQFYEGALEDGSDIEAQTTRD 600

Query: 601 WHAFRCYGPFCFFDLHEGKESQPPG-SGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVA 660
           WH +RC+GPFCFFD+HEGKESQ PG +GS VN+DE EFVLL+YH+LV  YPELKS+SQ+A
Sbjct: 601 WHKYRCFGPFCFFDIHEGKESQHPGATGSRVNLDEVEFVLLIYHRLVTMYPELKSSSQLA 660

Query: 661 IISPYSQQVKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFL 720
           IISPY+ QVK  +++FK+ FG +   +VDI +VDG QGREKD+AIFSCVRA+EN  IGFL
Sbjct: 661 IISPYNYQVKTFKDRFKEMFGTEAEKVVDINTVDGFQGREKDVAIFSCVRANENGQIGFL 720

Query: 721 SDCRRMNVGITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDS 780
           S+ RRMNVGITRA+SS+LVVGSA+TLK D  W NL+ESA++R+ LFKVSKP  +FFS+++
Sbjct: 721 SNSRRMNVGITRAKSSVLVVGSAATLKSDPLWKNLIESAEQRNRLFKVSKPLNNFFSEEN 780

Query: 781 IESMRVKAESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEED 821
           +E+M++  +  +      ++    VA  P  G        D+DFGDG+ D
Sbjct: 781 LETMKLTEDMEIPDAPLYEDESLPVA--PYGG--------DDDFGDGDAD 808

BLAST of MC08g0843 vs. ExPASy Swiss-Prot
Match: Q86AS0 (Probable helicase DDB_G0274399 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274399 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 9.3e-113
Identity = 309/888 (34.80%), Postives = 447/888 (50.34%), Query Frame = 0

Query: 11  ESVTSRLFKIILSWDYFRLLKDSKKRKDDDGSSLGLKEVKASYKDVDDYISTFEPLLLEE 70
           + +  R +K IL+WD   L   S K K+       LK VK S+ + +DYI+T+EPLL EE
Sbjct: 38  DQIIDRFYKHILTWDASDL---SPKEKE-------LKPVKVSFNNEEDYITTYEPLLFEE 97

Query: 71  VKAQII------QRNDDEEATDWKFRAIMECNE-----------VNGF------------ 130
            +AQ+       +++D  E T  + R I E N+           VN F            
Sbjct: 98  CRAQLERSIEEGEKDDTSEPTLSRVRYISEVNDFLVVGLVMAENVNIFQFHDNDLIMISL 157

Query: 131 HFPEIVYLREEE---LNDED----SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVE 190
           H P IV+  +E+    +DED    S      +P      +           TT    +++
Sbjct: 158 HHPLIVFGMDEDEEMTDDEDTAPTSAATHVGAPTKSTTTTTTTTTTTTTTTTTATTNIID 217

Query: 191 SRQQSKLRLR-------------------MYLAGEVTHKGVDAIVSSPRLLKVRSHITSS 250
              ++   ++                   ++L G V H     I       KV+ ++   
Sbjct: 218 DPNKTTEDIKKKKKVIPPSKTPITEQNRTLHLIGTVEHLDNGGI-------KVKFYVKGI 277

Query: 251 SKDGIYIYSL-----------KICSLSTIIREYIALWSISSLPFKDMILAAADKNTSKDQ 310
             D     SL           K+C+LST+ RE+ AL+  S   F   ++   D +     
Sbjct: 278 KGDRARQVSLLLRYEIDWWTTKLCNLSTLQREFAALYQCSQSNFMKTLM-MRDDDGEDGI 337

Query: 311 AWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSAILHAT- 370
             KI   L D      N+SQ  A+ + L      LIQGPPGTGKT  ILGL+S +LH+T 
Sbjct: 338 VMKIPPLLHDQFSSTYNDSQLNALTSALEGNAITLIQGPPGTGKTHVILGLISVLLHSTI 397

Query: 371 -PARMHSKIGLVE-TKQGSELPVREKYDHWIQASPWLNGINP--RDNIMPVNGDDGFFPT 430
            P        L +   +  EL + EK D W  + PW N   P  RDN   ++ D   F  
Sbjct: 398 VPKVKSGGNNLGDHLLKDRELSMAEKRDLWNISQPWFNKEFPHIRDNYELIDYD---FEE 457

Query: 431 SGNELKPEVVKSNR--------KYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTP 490
              + K ++ +  R          + R+L+CAPSN A+DEIV R+   G+ + +   Y P
Sbjct: 458 RDQKRKRDLWRKLRDTGSVKGGSTKRRILLCAPSNGAVDEIVSRLIRDGLLNADGRKYNP 517

Query: 491 KIVRIGLKAHPSIKTVSMKELVEQKK-----------NSMTTGKAKSGASGT--DLDSIR 550
            +VR+G  +H  +++VS+  +V  ++           +S +T  A SG+S +  D  SIR
Sbjct: 518 NLVRVGPGSHSDVESVSLDYMVRCRQQLMNSNSAIPSSSASTAAATSGSSRSTQDTSSIR 577

Query: 551 SAILDESVIVFSTLSFSGSSLFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVG 610
           + +LDE+ IV +TLSFSG+SL +K   GFD+VIIDEAAQAVE +TL+P+ +GCK+V LVG
Sbjct: 578 TLVLDEADIVATTLSFSGASLLTKMAGGFDIVIIDEAAQAVETSTLIPIQHGCKKVVLVG 637

Query: 611 DPEQLPATVISTTAKKFGYDKSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESL 670
           DP+QLPAT+IS  A K+ YD+SLF+R Q    P  ML  QYRMH  IR+FPSR FY + L
Sbjct: 638 DPKQLPATIISPLAIKYKYDQSLFQRLQEKNSP-HMLTTQYRMHSLIRAFPSRHFYQDLL 697

Query: 671 EDAPDVKLRTTRAWHAFRCYGPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVI 730
            D P++  R T  +H+   +GP  F+DL    E++ PG GS  N  E +  + L+     
Sbjct: 698 LDGPNIPSRATH-YHSNPFFGPLVFYDLSWSTETK-PGGGSVFNEHECKMAMYLFQLFTK 757

Query: 731 SYPELKSNSQVAIISPYSQQVKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSC 790
            YP+    S++ IISPY QQV  L+E FK+  G      + I +VDG QGRE++I IFSC
Sbjct: 758 VYPDEDFASRIGIISPYRQQVLALREIFKNYPG------ISIDTVDGFQGREREIIIFSC 817

Query: 791 VRA--SENRSIGFLSDCRRMNVGITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLF 799
           VRA   E   IGFLSD RRMNV +TR RSS+L++G+   L  ++ WN L++  Q    L 
Sbjct: 818 VRAPVEEGAGIGFLSDVRRMNVALTRPRSSLLILGNTKALSINKDWNELIQHTQNNQQLI 877

BLAST of MC08g0843 vs. ExPASy Swiss-Prot
Match: O94387 (Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC29A10.10c PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.1e-81
Identity = 242/809 (29.91%), Postives = 380/809 (46.97%), Query Frame = 0

Query: 18   FKIILSWDYFRLLKDSKKRKDDDGSSLGLKEVKASYKDVDDYISTFEPLLLEEVKAQIIQ 77
            +K+IL W+    L DS    + +   +   + K +Y D + Y   F+P+L  E  AQ+  
Sbjct: 1063 YKVILGWN---PLADSFSASNVE---MQCVQAKFTYNDSNAYEKVFKPMLFHECWAQV-- 1122

Query: 78   RNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDEDSEKGEFLSPNDLLLLSKEK 137
            ++  EE        I+        +F +I +            +  FLS  D+ LLSK +
Sbjct: 1123 KSAVEEKQYPPIDLILNTRSTVD-NFVDIYF------TSCSPTEVSFLSDTDICLLSKSQ 1182

Query: 138  FQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDAIVSSPRLLKVRSHITSSSK 197
               +   P ++    ++S  + K  L + L   +  + +D    +P              
Sbjct: 1183 SSGDTNNPKSFQLCKIQSISRKKESLELCLRMNI--ESIDLQEYAP-------------- 1242

Query: 198  DGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAA----ADKNTSKDQAWKISRPLQ 257
              I   + K+ + +T +RE+ AL S+  LP    IL A       N + D+  KI +   
Sbjct: 1243 -NIRFTAQKLFNATTSLREFAALKSLRHLPLSQRILDANVTRLPSNFTDDKKQKIMK--- 1302

Query: 258  DYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSAILHATPARMHSKIGL 317
                  +NE Q  A+ A      F LIQGPPGTGKT+TILG++ A+             L
Sbjct: 1303 ---SYGVNEPQAYAIYASSVNDGFTLIQGPPGTGKTKTILGMIGAV-------------L 1362

Query: 318  VETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFPTSGNELKPEVVKSNR 377
              + QG +                                   F   G        ++ +
Sbjct: 1363 TSSSQGLQ-----------------------------------FNVPG--------QTRK 1422

Query: 378  KYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIG----LKAH-------- 437
              + ++L+CAPSN+A+DEI+LR++  GV D     + PK++R+G    +  H        
Sbjct: 1423 TSKNKILICAPSNAAIDEILLRIK-AGVYDHEGIKFFPKVIRVGFGDSISVHAKEFTLEE 1482

Query: 438  --------------------------------------------------PSIKTVSMKE 497
                                                               SI    ++E
Sbjct: 1483 QMIKQMELTNLKKDQEANNSSDTRKKYDSIIKKRDSLREDLEKFRSTGKNSSILEAQLRE 1542

Query: 498  LVEQK---KNSMTTGKAKSGASGTDLD----SIRSAILDESVIVFSTLSFSGSSLFSKWN 557
            + +QK   + S+   + +  ++  +LD     I++ +L E+ IV +TLS SG  L     
Sbjct: 1543 ITKQKNMLEQSLDDMRERQRSTNRNLDVLKKQIQNQLLQEADIVCATLSASGHELLLNAG 1602

Query: 558  RGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYDKSLFKR 617
              F  VIIDEAAQAVEL++++PL  GC+   +VGDP QLP TV+S T+ KFGY +SL+ R
Sbjct: 1603 LTFRTVIIDEAAQAVELSSIIPLKYGCESCVMVGDPNQLPPTVLSKTSAKFGYSQSLYVR 1662

Query: 618  -FQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCYGPFCF 677
             F+       +L IQYRM+PEI  FPS+ FY   L D P++   T+R WH     G + F
Sbjct: 1663 MFKQHNESACLLSIQYRMNPEISRFPSKFFYNSKLLDGPNMSAVTSRPWHEDPQLGIYRF 1722

Query: 678  FDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQVKLLQ 737
            F++H G E+    S S  NV+EA F+LLLY +L+  Y  +    ++ +++PY  QV+ L+
Sbjct: 1723 FNVH-GTEAF-SNSKSLYNVEEASFILLLYERLIQCYLNIDFEGKIGVVTPYRSQVQQLR 1774

Query: 738  EKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNVGITRA 753
             +F+  +G      +DI +VDG QG+EKDI IFSCVR+S +  IGFL D RR+NV +TRA
Sbjct: 1783 SQFQRKYGSIIFKHLDIHTVDGFQGQEKDIIIFSCVRSSMSGGIGFLQDLRRLNVALTRA 1774

BLAST of MC08g0843 vs. ExPASy Swiss-Prot
Match: Q00416 (Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEN1 PE=1 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 8.8e-79
Identity = 242/812 (29.80%), Postives = 371/812 (45.69%), Query Frame = 0

Query: 17   LFKIILSWDYFRLLKDSKKRKDDDGSSLGLKEVKASYKDVDDYISTFEPLLLEEVKAQII 76
            L++IIL WDY R          DD       +VK  +    DY    +PLLL E    + 
Sbjct: 1116 LYEIILQWDYTR-----NSEYPDDEPIGNYSDVKDFFNSPADYQKVMKPLLLLESWQGLC 1175

Query: 77   QRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDEDSEKGEFLSPNDLLLLSKE 136
               D E   D+K  +I+  N      F ++     +++  +       +S +DL++++  
Sbjct: 1176 SSRDRE---DYKPFSIIVGNRTAVSDFYDVYASVAKQVIQDCG-----ISESDLIVMAYL 1235

Query: 137  KFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDAIVSSPRLLKVRSHITSSS 196
                  K  ++  F   +    +K+R      G      VD  +   R       +T  S
Sbjct: 1236 PDFRPDKRLSSDDFKKAQHTCLAKVRTLKNTKG----GNVDVTLRIHRNHSFSKFLTLRS 1295

Query: 197  KDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADKNTSKDQAWKISRPLQDYM 256
            +    IY +K+  ++TI REY  L  +        IL A         A +I    + Y 
Sbjct: 1296 E----IYCVKVMQMTTIEREYSTLEGLEYYDLVGQILQAKPSPPVNVDAAEIETVKKSY- 1355

Query: 257  KENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSAILHATPARMHSKIGLVET 316
               LN SQ  A+   +S++ F LIQGPPGTGKT+TILG++   L               T
Sbjct: 1356 --KLNTSQAEAIVNSVSKEGFSLIQGPPGTGKTKTILGIIGYFL--------------ST 1415

Query: 317  KQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFPTSGNELKPEVVKSNRKYR 376
            K  S                         N++ V       P   N    E +   +K  
Sbjct: 1416 KNASS-----------------------SNVIKV-------PLEKNSSNTEQLLKKQK-- 1475

Query: 377  VRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGLK--AHPSIKTVSMKELV- 436
              +L+CAPSN+A+DEI LR++ +GV D+  H + P++VR+G     + +IK ++++ELV 
Sbjct: 1476 --ILICAPSNAAVDEICLRLK-SGVYDKQGHQFKPQLVRVGRSDVVNVAIKDLTLEELVD 1535

Query: 437  ----------------EQKKNSMTT------GKAKSGAS--------------------- 496
                            E+K N+  T      GK  S +                      
Sbjct: 1536 KRIGERNYEIRTDPELERKFNNAVTKRRELRGKLDSESGNPESPMSTEDISKLQLKIREL 1595

Query: 497  -------GTDLDSIR------------------SAILDESVIVFSTLSFSGSSLFSKWNR 556
                   G D D +R                  + IL  S I+ STLS S   + +    
Sbjct: 1596 SKIINELGRDRDEMREKNSVNYRNRDLDRRNAQAHILAVSDIICSTLSGSAHDVLATMGI 1655

Query: 557  GFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYDKSLFKRF 616
             FD VIIDEA Q  EL++++PL  G K+  +VGDP QLP TV+S  A  F Y++SLF R 
Sbjct: 1656 KFDTVIIDEACQCTELSSIIPLRYGGKRCIMVGDPNQLPPTVLSGAASNFKYNQSLFVRM 1715

Query: 617  QTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCYGPFCFFD 676
            +    P  +L +QYRMHP I  FPS EFY   L+D P + +   R WH      P+ FFD
Sbjct: 1716 EKNSSPY-LLDVQYRMHPSISKFPSSEFYQGRLKDGPGMDILNKRPWHQLEPLAPYKFFD 1775

Query: 677  LHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYP-ELKSNSQVAIISPYSQQVKLLQE 736
            +  G++ Q   + S+ N++E    + L   L   +  ++    ++ IISPY +Q++ +++
Sbjct: 1776 IISGRQEQNAKTMSYTNMEEIRVAIELVDYLFRKFDNKIDFTGKIGIISPYREQMQKMRK 1835

Query: 737  KFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENR-SIGFLSDCRRMNVGITRA 756
            +F   FG   +  +D  ++DG QG+EK+I + SCVRA + + S+GFL D RRMNV +TRA
Sbjct: 1836 EFARYFGGMINKSIDFNTIDGFQGQEKEIILISCVRADDTKSSVGFLKDFRRMNVALTRA 1853

BLAST of MC08g0843 vs. ExPASy Swiss-Prot
Match: Q92355 (Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen1 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.7e-74
Identity = 203/667 (30.43%), Postives = 316/667 (47.38%), Query Frame = 0

Query: 202  IYSLKICSLSTIIREYIALWSISSLPF----KDMILAAADKNTSKDQAWKISRPLQDYMK 261
            ++ LK+ +L+T  R+Y     I  LP+     D+I A       K  + +I   ++ Y  
Sbjct: 1074 LWFLKLTNLATFTRQYA---GIRGLPYFHLADDIIRARPCSQPVKHSSSEIKAAMKRY-- 1133

Query: 262  ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSAILHATPARMHSKIGLVETK 321
              +NE Q  A+   L    F LIQGPPGTGKT+TI+G++SA+L    +R H         
Sbjct: 1134 -QVNEPQAKAIMCALDNNGFTLIQGPPGTGKTKTIIGIISALL-VDLSRYHI-------- 1193

Query: 322  QGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFPTSGNELKPEVVKSNRKYRV 381
                                                           +P     + + + 
Sbjct: 1194 ----------------------------------------------TRPNQQSKSTESKQ 1253

Query: 382  RVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIG--LKAHPSIKTVSM-----K 441
            ++L+CAPSN+A+DE++LR++  G   EN   Y P++VRIG     + S++ +S+     K
Sbjct: 1254 QILLCAPSNAAVDEVLLRLKR-GFLLENGEKYIPRVVRIGNPETINVSVRDLSLEYQTEK 1313

Query: 442  ELVEQKKNSMTTG----------------------------------------------- 501
            +L+E  + ++  G                                               
Sbjct: 1314 QLLEVNQGAIDLGSLQELTRWRDTFYDCIQKIEELEKQIDVARDVAEDTKSLGKELQNKI 1373

Query: 502  -------------KAKSGASGTDLDSIR----SAILDESVIVFSTLSFSGSSLFSKWNRG 561
                         +++S     ++D +R     AIL ++ +V +TLS SG  L +  +  
Sbjct: 1374 NEKNLAEQKVEELQSQSFTKNKEVDLLRKKAQKAILKQADVVCATLSGSGHDLVAHSSLN 1433

Query: 562  FDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYDKSLFKRFQ 621
            F  VIIDEAAQAVEL T++PL  G K+  LVGDP QLP TV+S  A    Y +SLF R Q
Sbjct: 1434 FSTVIIDEAAQAVELDTIIPLRYGAKKCILVGDPNQLPPTVLSKKAASLNYSQSLFVRIQ 1493

Query: 622  -TAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCYGPFCFFD 681
                  + +L IQYRMHP+I  FPS++FY   LED  ++  +T + WH    +  +  FD
Sbjct: 1494 KNFSNQMCLLSIQYRMHPDISHFPSKKFYDSRLEDGDNMAEKTQQVWHVNPKFTQYRLFD 1553

Query: 682  LHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQVKLLQEK 741
            +  GKE +   + S  N++E E+++ +  +L+  +P++    ++ +I+PY  Q+  L+  
Sbjct: 1554 V-RGKE-RTSNTMSTYNLEEVEYLVNMVDELLNKFPDVNFTGRIGVITPYRSQLHELRRA 1613

Query: 742  FKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNVGITRARS 789
            FK  +G      +DI +VDG QG+EKDI  FSCV++     IGFL D RR+NV +TRARS
Sbjct: 1614 FKVKYGKSFMSTIDIQTVDGFQGQEKDIIFFSCVKSYSKHGIGFLRDFRRLNVALTRARS 1673

BLAST of MC08g0843 vs. NCBI nr
Match: XP_023528758.1 (probable helicase MAGATAMA 3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1532 bits (3966), Expect = 0.0
Identity = 774/831 (93.14%), Postives = 805/831 (96.87%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDDDG--SSLGLKEVKASYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK+++ DDG  +SLGLKEVK+SYKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+A DWKFRAIM+C+EVNGFHFPEIVYLR+E++ DED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
            EK EFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHKGV+A
Sbjct: 121 GEKAEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHKGVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPF+DMILAAADK
Sbjct: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFRDMILAAADK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT +DQAWKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMHSK+GL+ET+QGSELPVREKYDHW QASPWLNGINPRDNIMPVNGDDGFFP
Sbjct: 301 ILHATPARMHSKVGLIETRQGSELPVREKYDHWNQASPWLNGINPRDNIMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKN+M TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNNMNTGKDKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPS+EFYAESLEDAPDVKLRT RAWHA+ CY
Sbjct: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSKEFYAESLEDAPDVKLRTARAWHAYHCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESA+KRDCL+KVSKPYTSF SD+SIESMRV +
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESARKRDCLYKVSKPYTSFLSDESIESMRVGS 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           ESAV PTGERDE++AN   EP AGDADQ   DDN+FGDG+EDMYEGGFEED
Sbjct: 781 ESAVGPTGERDESDANAPPEPNAGDADQAPADDNEFGDGDEDMYEGGFEED 831

BLAST of MC08g0843 vs. NCBI nr
Match: KAG6581597.1 (putative helicase MAGATAMA 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1527 bits (3954), Expect = 0.0
Identity = 774/831 (93.14%), Postives = 806/831 (96.99%), Query Frame = 0

Query: 1    MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDDDG--SSLGLKEVKASYKDVDD 60
            MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK+++ DDG  +SLGLKEVK+SYKDVDD
Sbjct: 774  MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 833

Query: 61   YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
            YISTFEPLLLEE+KAQIIQRNDDE+A DWKFRAIM+C+EVNGFHFPEIVYLR+E++ DED
Sbjct: 834  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 893

Query: 121  SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
             EK EFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHKGV+A
Sbjct: 894  GEKAEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHKGVEA 953

Query: 181  IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
            IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPF+DMILAAADK
Sbjct: 954  IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFRDMILAAADK 1013

Query: 241  NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
            NT +DQAWKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 1014 NTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 1073

Query: 301  ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
            ILHATPARMHSK+GL+ET+QGSELPVREKYDHW QASPWLNGINPRDNIMPVNGDDGFFP
Sbjct: 1074 ILHATPARMHSKVGLIETRQGSELPVREKYDHWNQASPWLNGINPRDNIMPVNGDDGFFP 1133

Query: 361  TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
            TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 1134 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 1193

Query: 421  KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
            K HPSIK VSMKELVEQKKN+M TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 1194 KPHPSIKAVSMKELVEQKKNNMNTGKDKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 1253

Query: 481  LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
            LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 1254 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 1313

Query: 541  KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
            KSLFKRFQ+AGYPVTMLKIQYRMHPEIRSFPS+EFYAESLEDAPDVKLRT RAWHA+ CY
Sbjct: 1314 KSLFKRFQSAGYPVTMLKIQYRMHPEIRSFPSKEFYAESLEDAPDVKLRTARAWHAYHCY 1373

Query: 601  GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
            GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 1374 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 1433

Query: 661  VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
            VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 1434 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 1493

Query: 721  GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
            GITRAR+SILVVGSASTLKRDEHWNNLVESA+KRDCL+KVSKPYTSF SD+SIESMRV +
Sbjct: 1494 GITRARASILVVGSASTLKRDEHWNNLVESARKRDCLYKVSKPYTSFLSDESIESMRVGS 1553

Query: 781  ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
            ESAV PTGERDE++AN A EP AGDADQ   DDN+FGDG+EDMYEGGFEED
Sbjct: 1554 ESAVGPTGERDESDAN-APEPNAGDADQAPADDNEFGDGDEDMYEGGFEED 1603

BLAST of MC08g0843 vs. NCBI nr
Match: XP_022935506.1 (probable helicase MAGATAMA 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1527 bits (3954), Expect = 0.0
Identity = 774/831 (93.14%), Postives = 806/831 (96.99%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDDDG--SSLGLKEVKASYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK+++ DDG  +SLGLKEVK+SYKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+A DWKFRAIM+C+EVNGFHFPEIVYLR+E++ DED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
            EK EFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHKGV+A
Sbjct: 121 GEKAEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHKGVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPF+DMILAAADK
Sbjct: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFRDMILAAADK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT +DQAWKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMHSK+GL+ET+QGSELPVREKYDHW QASPWLNGINPRDNIMPVNGDDGFFP
Sbjct: 301 ILHATPARMHSKVGLIETRQGSELPVREKYDHWNQASPWLNGINPRDNIMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKN+M TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNNMNTGKDKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQ+AGYPVTMLKIQYRMHPEIRSFPS+EFYAESLEDAPDVKLRT RAWHA+ CY
Sbjct: 541 KSLFKRFQSAGYPVTMLKIQYRMHPEIRSFPSKEFYAESLEDAPDVKLRTARAWHAYHCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESA+KRDCL+KVSKPYTSF SD+SIESMRV +
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESARKRDCLYKVSKPYTSFLSDESIESMRVGS 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           ESAV PTGERDE++AN A EP AGDADQ   DDN+FGDG+EDMYEGGFEED
Sbjct: 781 ESAVGPTGERDESDAN-APEPNAGDADQAPADDNEFGDGDEDMYEGGFEED 830

BLAST of MC08g0843 vs. NCBI nr
Match: KAG7018098.1 (putative helicase MAGATAMA 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1526 bits (3950), Expect = 0.0
Identity = 773/831 (93.02%), Postives = 805/831 (96.87%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDDDG--SSLGLKEVKASYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK+++ DDG  +SLGLKEVK+SYKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+A DWKFRAIM+C+EVNGFHFPEIVYLR+E++ DED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
            EK EFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHKGV+A
Sbjct: 121 GEKAEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHKGVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPF+DMILAAADK
Sbjct: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFRDMILAAADK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT +DQAWKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMHSK+GL+ET+QGSELPVREKYDHW QASPWLNGINPRDNIMPVNGDDGFFP
Sbjct: 301 ILHATPARMHSKVGLIETRQGSELPVREKYDHWNQASPWLNGINPRDNIMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKN+M TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNNMNTGKDKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQ+AGYPVTMLKIQYRMHPEIRSFPS+EFYAESLEDAPDVKLRT RAWHA+ CY
Sbjct: 541 KSLFKRFQSAGYPVTMLKIQYRMHPEIRSFPSKEFYAESLEDAPDVKLRTARAWHAYHCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESA+KRDCL+KVSKPYTSF SD+SIESMRV +
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESARKRDCLYKVSKPYTSFLSDESIESMRVGS 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           ES V PTGERDE++AN A EP AGDADQ   DDN+FGDG+EDMYEGGFEED
Sbjct: 781 ESVVGPTGERDESDAN-APEPNAGDADQAPADDNEFGDGDEDMYEGGFEED 830

BLAST of MC08g0843 vs. NCBI nr
Match: XP_038881790.1 (probable helicase MAGATAMA 3 isoform X2 [Benincasa hispida] >XP_038881791.1 probable helicase MAGATAMA 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 1524 bits (3947), Expect = 0.0
Identity = 769/831 (92.54%), Postives = 807/831 (97.11%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDD--DGSSLGLKEVKASYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLK+SKK K+D  DG+SLGLKEVK++YKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKNSKKHKEDVGDGASLGLKEVKSTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           YISTFEPLLLEE+KAQIIQRNDDEEA DWKFRAI+EC+EVNGFHFPEIVYLR+EELNDED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEEAADWKFRAIVECSEVNGFHFPEIVYLRDEELNDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
           S KGEFLSPNDLLLLSKEKFQENA+LPTTYAFALVESRQQSKLRLRMYLAGEVTHKGV+A
Sbjct: 121 SGKGEFLSPNDLLLLSKEKFQENARLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPF++MILAAADK
Sbjct: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFREMILAAADK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT KDQAWKISRPLQ+YM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGKDQAWKISRPLQEYMQENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMH+KIGL+ET+QGSELPVREKY+HW QASPWLNGINPRDN+MPVNGDDGFFP
Sbjct: 301 ILHATPARMHTKIGLIETRQGSELPVREKYNHWNQASPWLNGINPRDNLMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           T+GNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDH YTPKIVRIGL
Sbjct: 361 TTGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHSYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKNSM TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNSMNTGKEKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQ+AGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHA+RCY
Sbjct: 541 KSLFKRFQSAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAYRCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVN+DEAEFV+LLY+KLV SYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNIDEAEFVILLYYKLVTSYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKF DTFG+DPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFTDTFGMDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESA+KRDCL+KVSKPYT+F SD+++ESMRVK 
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESARKRDCLYKVSKPYTTFLSDENVESMRVKN 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           E  V PTG+RDETEAN  QEP AGDA+Q Q D+NDFGDGEE+MYEGGFEED
Sbjct: 781 EPPVGPTGDRDETEANAPQEPNAGDANQAQADENDFGDGEEEMYEGGFEED 831

BLAST of MC08g0843 vs. ExPASy TrEMBL
Match: A0A6J1F4V5 (probable helicase MAGATAMA 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442358 PE=4 SV=1)

HSP 1 Score: 1527 bits (3954), Expect = 0.0
Identity = 774/831 (93.14%), Postives = 806/831 (96.99%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDDDG--SSLGLKEVKASYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK+++ DDG  +SLGLKEVK+SYKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+A DWKFRAIM+C+EVNGFHFPEIVYLR+E++ DED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
            EK EFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHKGV+A
Sbjct: 121 GEKAEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHKGVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPF+DMILAAADK
Sbjct: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFRDMILAAADK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT +DQAWKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMHSK+GL+ET+QGSELPVREKYDHW QASPWLNGINPRDNIMPVNGDDGFFP
Sbjct: 301 ILHATPARMHSKVGLIETRQGSELPVREKYDHWNQASPWLNGINPRDNIMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKN+M TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNNMNTGKDKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQ+AGYPVTMLKIQYRMHPEIRSFPS+EFYAESLEDAPDVKLRT RAWHA+ CY
Sbjct: 541 KSLFKRFQSAGYPVTMLKIQYRMHPEIRSFPSKEFYAESLEDAPDVKLRTARAWHAYHCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESA+KRDCL+KVSKPYTSF SD+SIESMRV +
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESARKRDCLYKVSKPYTSFLSDESIESMRVGS 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           ESAV PTGERDE++AN A EP AGDADQ   DDN+FGDG+EDMYEGGFEED
Sbjct: 781 ESAVGPTGERDESDAN-APEPNAGDADQAPADDNEFGDGDEDMYEGGFEED 830

BLAST of MC08g0843 vs. ExPASy TrEMBL
Match: A0A6J1J637 (probable helicase MAGATAMA 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482100 PE=4 SV=1)

HSP 1 Score: 1524 bits (3946), Expect = 0.0
Identity = 771/831 (92.78%), Postives = 802/831 (96.51%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDDDG--SSLGLKEVKASYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLK+SK+++ DDG  +SLGLKEVK+SYKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKESKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+  DWKFRAIM+C+EVNGFHFPEIVYLR+E++ DED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDVADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
            EK EFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHKGV+A
Sbjct: 121 GEKAEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHKGVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPF+DMILAAADK
Sbjct: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFRDMILAAADK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT +DQAWKISR LQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGEDQAWKISRSLQDYMRENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMHSK+GL+ET+QGSELPVREKYDHW QASPWLNGINPRDNIMPVNGDDGFFP
Sbjct: 301 ILHATPARMHSKVGLIETRQGSELPVREKYDHWNQASPWLNGINPRDNIMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKN+M TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNNMNTGKDKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPS+EFYAESLEDAPDVKLRT RAWHA+ CY
Sbjct: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSKEFYAESLEDAPDVKLRTARAWHAYHCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESA+KRDCL+KVSKPYTSF SD+SIESMRV +
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESARKRDCLYKVSKPYTSFLSDESIESMRVGS 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           ESAV PTGERDE+ AN   EP AGDADQ   DDN+FGDG+EDMYEGGFEED
Sbjct: 781 ESAVGPTGERDESGANAPPEPNAGDADQAPADDNEFGDGDEDMYEGGFEED 831

BLAST of MC08g0843 vs. ExPASy TrEMBL
Match: A0A5A7UB64 (Putative helicase MAGATAMA 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2510G00490 PE=4 SV=1)

HSP 1 Score: 1522 bits (3940), Expect = 0.0
Identity = 769/831 (92.54%), Postives = 800/831 (96.27%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDDDG--SSLGLKEVKASYKDVDD 60
           MAVDKEK  EESVTSRLFKIILSWDYFRLLK+SKKRK+DDG  +SLGLKEVK++YKDVDD
Sbjct: 1   MAVDKEKAVEESVTSRLFKIILSWDYFRLLKNSKKRKEDDGDGASLGLKEVKSTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           Y STFEPLLLEE+KAQIIQRN+DEEA+DWKFRAIMECNEVNGFHFPE+VYLR+E+L DED
Sbjct: 61  YTSTFEPLLLEEIKAQIIQRNEDEEASDWKFRAIMECNEVNGFHFPEMVYLRDEDLKDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
           SEKG+FLSPNDLLLLSKEKFQEN KLPTTYAFALVESRQQSKLRLRMYLAGEVTHK V+A
Sbjct: 121 SEKGDFLSPNDLLLLSKEKFQENTKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKDVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           I+SSPRLLKV+SHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFK+MILAAADK
Sbjct: 181 IISSPRLLKVQSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKEMILAAADK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT KDQAWKIS+PLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGKDQAWKISKPLQDYMQENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMHS IGL+ET+ GSELPVREKYDHW QASPWLNGINPRDN+MPVNGDDGFFP
Sbjct: 301 ILHATPARMHSTIGLIETRHGSELPVREKYDHWNQASPWLNGINPRDNLMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKN+M TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNNMNTGKEKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHA+RCY
Sbjct: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAYRCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVNVDEA+FVL LYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKF D FG+DPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFVDLFGMDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYT+F SD S+ESMRVK 
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTTFLSDVSVESMRVKN 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           E  V P GE+DETEAN  QEP AGDADQ Q DDNDFGDG+E+MYEGGFEED
Sbjct: 781 EPPVGPVGEKDETEANALQEPNAGDADQAQADDNDFGDGDEEMYEGGFEED 831

BLAST of MC08g0843 vs. ExPASy TrEMBL
Match: A0A1S3CEF6 (probable helicase MAGATAMA 3 OS=Cucumis melo OX=3656 GN=LOC103499555 PE=4 SV=1)

HSP 1 Score: 1522 bits (3940), Expect = 0.0
Identity = 769/831 (92.54%), Postives = 800/831 (96.27%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKDDDG--SSLGLKEVKASYKDVDD 60
           MAVDKEK  EESVTSRLFKIILSWDYFRLLK+SKKRK+DDG  +SLGLKEVK++YKDVDD
Sbjct: 1   MAVDKEKAVEESVTSRLFKIILSWDYFRLLKNSKKRKEDDGDGASLGLKEVKSTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           Y STFEPLLLEE+KAQIIQRN+DEEA+DWKFRAIMECNEVNGFHFPE+VYLR+E+L DED
Sbjct: 61  YTSTFEPLLLEEIKAQIIQRNEDEEASDWKFRAIMECNEVNGFHFPEMVYLRDEDLKDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
           SEKG+FLSPNDLLLLSKEKFQEN KLPTTYAFALVESRQQSKLRLRMYLAGEVTHK V+A
Sbjct: 121 SEKGDFLSPNDLLLLSKEKFQENTKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKDVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           I+SSPRLLKV+SHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFK+MILAAADK
Sbjct: 181 IISSPRLLKVQSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKEMILAAADK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT KDQAWKIS+PLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGKDQAWKISKPLQDYMQENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMHS IGL+ET+ GSELPVREKYDHW QASPWLNGINPRDN+MPVNGDDGFFP
Sbjct: 301 ILHATPARMHSTIGLIETRHGSELPVREKYDHWNQASPWLNGINPRDNLMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKN+M TGK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNNMNTGKEKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHA+RCY
Sbjct: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAYRCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVNVDEA+FVL LYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEADFVLHLYHKLVISYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKF D FG+DPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFVDLFGMDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYT+F SD S+ESMRVK 
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTTFLSDVSVESMRVKN 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           E  V P GE+DETEAN  QEP AGDADQ Q DDNDFGDG+E+MYEGGFEED
Sbjct: 781 EPPVGPVGEKDETEANALQEPNAGDADQAQADDNDFGDGDEEMYEGGFEED 831

BLAST of MC08g0843 vs. ExPASy TrEMBL
Match: A0A0A0KM05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523480 PE=4 SV=1)

HSP 1 Score: 1512 bits (3915), Expect = 0.0
Identity = 764/831 (91.94%), Postives = 798/831 (96.03%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRK--DDDGSSLGLKEVKASYKDVDD 60
           MAVDKEK  EESVTSRLFKIILSWDYFRLLK+SKKRK  D DG+SLGLKEVK++YKDVDD
Sbjct: 1   MAVDKEKAVEESVTSRLFKIILSWDYFRLLKNSKKRKENDGDGASLGLKEVKSTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           Y STFEPLLLEE+KAQIIQRN+DEEA+DWKFRAIMEC+EVNGFHFPE+VYLR+E+L DED
Sbjct: 61  YTSTFEPLLLEEIKAQIIQRNEDEEASDWKFRAIMECSEVNGFHFPEMVYLRDEDLKDED 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
           SEKG+FLSPNDLLLLSKEKFQEN KLPTTYAFALVESRQQSKLRLRMYLAGEVTHK V+A
Sbjct: 121 SEKGDFLSPNDLLLLSKEKFQENTKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKDVEA 180

Query: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAADK 240
           IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFK+MILAA DK
Sbjct: 181 IVSSPRLLKVRSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKEMILAATDK 240

Query: 241 NTSKDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300
           NT KDQAWKIS+PLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA
Sbjct: 241 NTGKDQAWKISKPLQDYMQENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 300

Query: 301 ILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFP 360
           ILHATPARMHS IGL+ET+ GSELPVREKYDHW QASPWLNGINPRD++MPVNGDDGFFP
Sbjct: 301 ILHATPARMHSTIGLIETRHGSELPVREKYDHWNQASPWLNGINPRDDLMPVNGDDGFFP 360

Query: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420
           TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL
Sbjct: 361 TSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL 420

Query: 421 KAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480
           K HPSIK VSMKELVEQKKN+M+ GK KSGASGTDLDSIRSAILDESVIVFSTLSFSGSS
Sbjct: 421 KPHPSIKAVSMKELVEQKKNNMSMGKEKSGASGTDLDSIRSAILDESVIVFSTLSFSGSS 480

Query: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540
           LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD
Sbjct: 481 LFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTTAKKFGYD 540

Query: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCY 600
           KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDA DVKLRTTRAWHA+RCY
Sbjct: 541 KSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAQDVKLRTTRAWHAYRCY 600

Query: 601 GPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQ 660
           GPFCFFDLHEGKESQPPGSGSWVN+DEA+FVL LYHKLVISYPELKSNSQVAIISPYSQQ
Sbjct: 601 GPFCFFDLHEGKESQPPGSGSWVNIDEADFVLHLYHKLVISYPELKSNSQVAIISPYSQQ 660

Query: 661 VKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720
           VKLLQEKF D FG+DPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV
Sbjct: 661 VKLLQEKFVDLFGMDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFLSDCRRMNV 720

Query: 721 GITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDSIESMRVKA 780
           GITRAR+SILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYT+F +D+S+ESMRVK 
Sbjct: 721 GITRARASILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTTFLNDESVESMRVKN 780

Query: 781 ESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEEDMYEGGFEED 829
           E  V P GE+DETEAN  QEP AGDADQ Q DDNDFGDG+E+MYEGGFEED
Sbjct: 781 EPPVGPMGEKDETEANAQQEPNAGDADQAQADDNDFGDGDEEMYEGGFEED 831

BLAST of MC08g0843 vs. TAIR 10
Match: AT4G15570.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 938.3 bits (2424), Expect = 4.1e-273
Identity = 499/830 (60.12%), Postives = 625/830 (75.30%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTS--RLFKIILSWDYFRLLKDSKKRKDDDGSSLGLKEVKASYKDVDD 60
           MA+D  K+ EE  +S  R + IIL WDY +L K++ +RK+   S   L  VK +YKDVDD
Sbjct: 1   MAIDNGKLQEEEASSVTRFYNIILGWDYKQLTKEN-ERKNRKDSKEKLNVVKNTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDED 120
           Y  TFEPLL EEVKAQI+Q  D EEA+  K R +MECNE  GFHF  + Y  EE+     
Sbjct: 61  YFETFEPLLFEEVKAQILQNKDGEEASVCKMRLVMECNEGEGFHFLLVTYEHEED----- 120

Query: 121 SEKGEFLSPNDLLLLSKEKFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVDA 180
               E+L+ NDLLLLSKE+ + N+  P++Y FA+VE RQ + LRLRMYLA ++     + 
Sbjct: 121 ----EYLAQNDLLLLSKEEVKGNS-FPSSYGFAVVEHRQNNLLRLRMYLAEDIVQITKNT 180

Query: 181 IVSSPR-----LLKVRSHITSS-SKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMI 240
             S  +     L  +RS ITSS S     ++SLK+C LSTIIREYIAL S+SSLPFKD+I
Sbjct: 181 KSSRTKSFIQALSNMRSLITSSASPIDKRVFSLKLCGLSTIIREYIALRSVSSLPFKDLI 240

Query: 241 LAAADKNTS-KDQAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQT 300
             AA+K+    D+AWKIS PL ++  ENLN+SQ+ A+  GLSRK FVLIQGPPGTGKTQT
Sbjct: 241 FTAAEKSCGFGDEAWKISGPLNEFFNENLNKSQKEAIDVGLSRKSFVLIQGPPGTGKTQT 300

Query: 301 ILGLLSAILHATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVN 360
           IL +L AI+HATPAR+ SK    E K+G ++ ++EKY+HW +ASPW+ G+NPRD IMP +
Sbjct: 301 ILSILGAIMHATPARVQSKGTDHEVKRGIQMTIQEKYNHWGRASPWILGVNPRDAIMPED 360

Query: 361 GDDGFFPTSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTP 420
           GDDGFFPTSGNELKPEVV ++RKYR+RVLVCAPSNSALDEIVLR+ ++G+RDEN   YTP
Sbjct: 361 GDDGFFPTSGNELKPEVVNASRKYRLRVLVCAPSNSALDEIVLRLLSSGLRDENAQTYTP 420

Query: 421 KIVRIGLKAHPSIKTVSMKELVEQKKNSMTTGKAKSGASGTDLDSIRSAILDESVIVFST 480
           KIVRIGLKAH S+ +VS+  LV QK+ S    K K G +GTD+DSIR+AIL+E+ IVF+T
Sbjct: 421 KIVRIGLKAHHSVASVSLDHLVAQKRGS-AIDKPKQGTTGTDIDSIRTAILEEAAIVFAT 480

Query: 481 LSFSGSSLFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQLPATVISTT 540
           LSFSGS+L +K NRGFDVVIIDEAAQAVE ATL+PLA  CKQVFLVGDP+QLPATVIST 
Sbjct: 481 LSFSGSALLAKSNRGFDVVIIDEAAQAVEPATLIPLATRCKQVFLVGDPKQLPATVISTV 540

Query: 541 AKKFGYDKSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRA 600
           A+  GY  S+F+R Q AGYPV MLK QYRMHPEIRSFPS++FY  +LED  D++ +TTR 
Sbjct: 541 AQDSGYGTSMFERLQKAGYPVKMLKTQYRMHPEIRSFPSKQFYEGALEDGSDIEAQTTRD 600

Query: 601 WHAFRCYGPFCFFDLHEGKESQPPG-SGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVA 660
           WH +RC+GPFCFFD+HEGKESQ PG +GS VN+DE EFVLL+YH+LV  YPELKS+SQ+A
Sbjct: 601 WHKYRCFGPFCFFDIHEGKESQHPGATGSRVNLDEVEFVLLIYHRLVTMYPELKSSSQLA 660

Query: 661 IISPYSQQVKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIAIFSCVRASENRSIGFL 720
           IISPY+ QVK  +++FK+ FG +   +VDI +VDG QGREKD+AIFSCVRA+EN  IGFL
Sbjct: 661 IISPYNYQVKTFKDRFKEMFGTEAEKVVDINTVDGFQGREKDVAIFSCVRANENGQIGFL 720

Query: 721 SDCRRMNVGITRARSSILVVGSASTLKRDEHWNNLVESAQKRDCLFKVSKPYTSFFSDDS 780
           S+ RRMNVGITRA+SS+LVVGSA+TLK D  W NL+ESA++R+ LFKVSKP  +FFS+++
Sbjct: 721 SNSRRMNVGITRAKSSVLVVGSAATLKSDPLWKNLIESAEQRNRLFKVSKPLNNFFSEEN 780

Query: 781 IESMRVKAESAVRPTGERDETEANVAQEPTAGDADQVQPDDNDFGDGEED 821
           +E+M++  +  +      ++    VA  P  G        D+DFGDG+ D
Sbjct: 781 LETMKLTEDMEIPDAPLYEDESLPVA--PYGG--------DDDFGDGDAD 808

BLAST of MC08g0843 vs. TAIR 10
Match: AT1G16800.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 1.1e-87
Identity = 258/898 (28.73%), Postives = 411/898 (45.77%), Query Frame = 0

Query: 18   FKIILSWDYFRLLKDSKKRKDDDGSSLGLKEVKASYKDVDDYISTFEPLLLEEVKAQI-- 77
            F+ IL  DY+ ++  +  +KD+  +    +EV   +   + YI  F+PL+LEE KAQ+  
Sbjct: 1058 FRKILQMDYYAIVGLASTKKDESQNVGKFREVPVRFGSPEQYIQIFQPLVLEEFKAQLQS 1117

Query: 78   -IQRNDDEEATDWKFRAIMECNEVNGFHFPEIVYLREEELNDEDSEKGEFLSPNDLLLLS 137
              Q     E   +   +++    V+ FHF  + ++++E     D    +  S NDL+L +
Sbjct: 1118 SFQEISSLEEIYYGVISVLSIERVDDFHF--VRFMQDE----NDGSNSKSFSENDLVLFT 1177

Query: 138  KEKFQENAKLPTTYAFALVESRQ------QSKLRLRMYLAGEVTHKGVDAIVSSPRLLKV 197
            KE   EN+ +        VE R+       S L +R+YL             +S RL + 
Sbjct: 1178 KE-HPENSNVGVN-MMGKVEGREWDDKKRTSILNVRLYLQN-----------ASSRLNQA 1237

Query: 198  RSHITSSSKDGIYIYSLKICSLSTIIREYIALWSISSLPFKDMILAAA-----DKNTSKD 257
            R ++   S+     ++ +I ++++ IRE+ AL  I  +P   +IL+       D    + 
Sbjct: 1238 RRNLLERSQ----WHASRILNITSQIREFQALSCIKDIPVLPLILSPMNDSNYDSEVKRS 1297

Query: 258  QAWKISRPLQDYMKENLNESQQAAVQAGLSRKPFV------LIQGPPGTGKTQTILGLLS 317
                +   LQ  +K + NESQ  A+   +     +      LIQGPPGTGKT+TI+ ++S
Sbjct: 1298 DLRSLPHSLQQILKSSFNESQLQAISVAIGSSNLMKAFDISLIQGPPGTGKTRTIVAIIS 1357

Query: 318  AIL----HATPARMHSKIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGD 377
             +L    H T  R +S+ G                     +S    G+NP   +     D
Sbjct: 1358 GLLASASHKTSDRGNSEPG-------------------HSSSTSRQGMNPSVAVARAWQD 1417

Query: 378  DGFFPTSGNELKPEVVKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKI 437
                    +  +     + +  R RVL+CA SN+A+DE+V R+ + G+   +   + P +
Sbjct: 1418 AALAKQLNDNSETNRKIAEKNGRGRVLICAQSNAAVDELVSRISSLGIYGRDGKMFKPYL 1477

Query: 438  VRIG--LKAHPSIKTVSMKELVEQK----KNSMTTGKAKSGASGTDL------------- 497
            VR+G     H +     +  LV+Q+    +  +   K+  GA  + L             
Sbjct: 1478 VRVGNAKTVHSNSMPFFLDTLVDQRLAEERMRINESKSNKGADSSALLRSNLEKVVDQIT 1537

Query: 498  ------------------------------------------------------------ 557
                                                                        
Sbjct: 1538 HFEAKRANINQESLDAKDKPENEHHNKDDDGKLMSDAELGIRLRRLYEQKRKIYKDLSAV 1597

Query: 558  ---------------DSIRSAILDESVIVFSTLSFSGSSLFS--------------KWNR 617
                             +R +IL E+ IV +TLS  G  L+S                + 
Sbjct: 1598 QAQERKANYEMRTLKQKLRKSILKEAQIVVTTLSGCGGDLYSVCAESLAAHKFGSPSEDN 1657

Query: 618  GFDVVIIDEAAQAVELATLVPL----ANGCKQVFLVGDPEQLPATVISTTAKKFGYDKSL 677
             FD V+IDEAAQA+E ATL+PL    + G K + +VGDP+QLPATV+S  A KF Y+ S+
Sbjct: 1658 LFDAVVIDEAAQALEPATLIPLQLLKSRGTKCI-MVGDPKQLPATVLSNVASKFLYECSM 1717

Query: 678  FKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPDVKLRTTRAWHAFRCYGPF 737
            F+R Q AGYP+ ML  QYRMHPEI  FPS  FY   L +  D+  ++   +H     GP+
Sbjct: 1718 FERLQRAGYPILMLTQQYRMHPEICRFPSMHFYDNKLLNGVDMSSKSA-PFHENHHLGPY 1777

Query: 738  CFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPELKSNSQVAIISPYSQQVKL 773
             F+D+ +G+E +   S S  N  EAE  + L       YP      ++ II+PY +Q+ +
Sbjct: 1778 VFYDIVDGQEHRSGDSSSVCNEQEAEAAVQLLRFFKKRYPSEFVAGRIGIITPYKRQLAV 1837

BLAST of MC08g0843 vs. TAIR 10
Match: AT4G30100.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-79
Identity = 248/837 (29.63%), Postives = 377/837 (45.04%), Query Frame = 0

Query: 46   LKEVKASYKDVDDYISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPE 105
            L+ V   ++ +D+Y+  FEPLL EE +AQ+         + W+     E  E N +    
Sbjct: 418  LQSVPERFESMDEYVRVFEPLLFEECRAQLY--------STWE-----ELAEANSYMKVR 477

Query: 106  IVYLREEE-------LNDEDSEKGEFLSPNDLLLLSK-------EKFQENAKLPTTYAFA 165
            I ++   E       LN  +  K  F    D+ +LS        E         T   + 
Sbjct: 478  IKFIERRERGWYDVILNSVNECKWAF-KEGDVAVLSNPVPESEGEHDDVGRVAGTVRRYI 537

Query: 166  LVESRQQSKLRLRMYLAGEVTHKGVDAIVSSPRLLKVRSHITSSSKDGIYIYSLKICSLS 225
             V++R      L  Y+         DA  S  ++    +HI    K     +   + S++
Sbjct: 538  PVDTRDPHGAILHFYVG--------DAYDSGSKI--DDNHILRKLKPKEIWHLTVLGSIA 597

Query: 226  TIIREYIALWSISSL--PFKDMILAAADKNTSKDQAWKISRP------LQDYMKENLNES 285
            T  REY+AL + S L    ++ IL  + +          + P         ++  + N  
Sbjct: 598  TTQREYVALHAFSQLNPQMQNAILKPSPEQFPNYGEQTPTVPDCFTPSFAGHLHRSFNAP 657

Query: 286  QQAAV-----------QAGLSRK---PFVLIQGPPGTGKTQTILGLLSAILHATPARMHS 345
            Q AA+            +G+ R+   PF L+QGPPGTGKT T+ G+L+ ++H    + + 
Sbjct: 658  QLAAIHWAAMHTAAGTSSGVKRQDPWPFTLVQGPPGTGKTHTVWGMLN-VIHLVQYQQYY 717

Query: 346  KIGLVETKQGSELPVRE-KYDHWIQASPWLNGINPRDNIMPVNGDDGFFPTSGNELKPEV 405
               L +    S   V E   D+ +  S         D ++  N D   F T      P++
Sbjct: 718  TSLLKKLAPESYKQVNESSSDNIVSGS--------IDEVLQ-NMDQNLFRT-----LPKL 777

Query: 406  VKSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGLKAH-PSIKTV 465
                   + R+LVCAPSN+A DE++ RV + G  D     Y P + R+G+     + + V
Sbjct: 778  CA-----KPRMLVCAPSNAATDELLSRVLDRGFIDGEMRVYRPDVARVGVDTQTKAAQAV 837

Query: 466  SM---------------------------------------------------------- 525
            S+                                                          
Sbjct: 838  SVERRSDLLLAKSREEILGHIHNLRVRDAQLSQDIAGLKRELTAAAFANRSQGSVGVDPD 897

Query: 526  ------------------------KELVEQKKNSMTTGKAKSGASGTDLD---SIRSAIL 585
                                    K+LVE  +  +  GK ++G S    +   S+ ++  
Sbjct: 898  VLMVRDQTRDAMLQRLSAVVEARDKDLVEMSRLLIVEGKFRAGTSFNLEEARASLEASFA 957

Query: 586  DESVIVFSTLSFSGSSLFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQ 645
            +E+ IVF+T+S SG  LFS+   GFD+V+IDEAAQA E+  L PLA G  +  LVGDP+Q
Sbjct: 958  NEAEIVFTTVSSSGRKLFSRLTHGFDMVVIDEAAQASEVGVLPPLALGAARCVLVGDPQQ 1017

Query: 646  LPATVISTTAKKFGYDKSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAP 705
            LPATVIS  A    Y +SLF+RFQ AG P  +L +QYRMHP+IR FPSR FY   L D+ 
Sbjct: 1018 LPATVISKAAGTLLYSRSLFERFQLAGCPTLLLTVQYRMHPQIRDFPSRYFYQGRLTDSE 1077

Query: 706  DVKLRTTRAWHAFRCYGPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPE 759
             V       ++      P+ FFD+  G+ES   GS S+ N+DEA F + +Y  L  +   
Sbjct: 1078 SVSTAPDEIYYKDSVLKPYLFFDISHGRESHRGGSVSYENIDEARFCVGVYLHLQRTLKS 1137

BLAST of MC08g0843 vs. TAIR 10
Match: AT2G19120.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 2.4e-79
Identity = 241/836 (28.83%), Postives = 379/836 (45.33%), Query Frame = 0

Query: 46   LKEVKASYKDVDDYISTFEPLLLEEVKAQIIQRNDDEEATDWKFRAIMECNEVNGFHFPE 105
            L+ V   ++ +++Y+  FEPLL EE +AQ+             +   +E  E N +    
Sbjct: 254  LRSVPERFESMEEYVRVFEPLLFEECRAQL-------------YSTWVELAEANTYVKVR 313

Query: 106  IVYLREEE-------LNDEDSEKGEFLSPNDLLLL------SKEKFQENAKLPTTYAFAL 165
            I  +   E       LN  +  K  F    D+ +L      S E  ++  ++  T    +
Sbjct: 314  IKSIERRERGWYDVILNSLNGCKWAF-KEGDVAVLSTPLPESDEDHEDAGRVAGTVRRHI 373

Query: 166  -VESRQQSKLRLRMYLAGEVTHKGVDAIVSSPRLLKVRSHITSSSKDGIYIYSLKICSLS 225
             V++R      L  Y+       G  + +         SHI    K     +   + SL+
Sbjct: 374  PVDTRDPRGATLHFYVGNS---GGTGSKIDD-------SHILRKLKPQDIWHLTVLGSLA 433

Query: 226  TIIREYIALWSISSL--PFKDMILAAADKNTSKDQAWKISRP------LQDYMKENLNES 285
            T  REY+AL + S L    ++ IL  + +     +    + P        D++  + N  
Sbjct: 434  TTQREYVALHAFSRLNPQMQNAILNPSPEQFPSYEEQTPATPDCFTTSFVDHLHRSFNAP 493

Query: 286  QQAAV-----------QAGLSRK---PFVLIQGPPGTGKTQTILGLLSAILHATPARMHS 345
            Q AA+            +G+ ++   PF L+QGPPGTGKT T+ G+L+ ++H    + + 
Sbjct: 494  QLAAIHWAAMHTAAGTSSGVKKQEPWPFTLVQGPPGTGKTHTVWGMLN-VIHLVQYQQYY 553

Query: 346  KIGLVETKQGSELPVREKYDHWIQASPWLNGINPRDNIMPVNGDDGFFPTSGNELKPEVV 405
               L +          E Y+   + S   N ++   + +  N D   F T      P++ 
Sbjct: 554  TSLLKKL-------APETYNQANECSSSDNILSGSIDEVLQNMDHNLFRT-----LPKLC 613

Query: 406  KSNRKYRVRVLVCAPSNSALDEIVLRVQNTGVRDENDHPYTPKIVRIGL----------- 465
                  + R+LVCAPSN+A DE++ RV + G  D     Y P + R+G+           
Sbjct: 614  A-----KPRMLVCAPSNAATDELLSRVLDRGFIDGEMRVYRPDVARVGVDSQSRAAQAVS 673

Query: 466  --KAHPSIKTVSMKEL--------------------VEQKKNSMTTGKAKSGASGTDLD- 525
              +    +  +S  E+                    ++++ N+        G+ G D + 
Sbjct: 674  VERRSDQLLAISRDEILRHMRNLRLQETQISQNIAGLKRELNAAAFATRSQGSVGVDPEV 733

Query: 526  ----------------------------------------------------SIRSAILD 585
                                                                S+ ++  +
Sbjct: 734  LISRDQKRDALLQHLAAVVEARDKVLVEISRLLIVEGKFRAGNNFNLEEARASLEASFAN 793

Query: 586  ESVIVFSTLSFSGSSLFSKWNRGFDVVIIDEAAQAVELATLVPLANGCKQVFLVGDPEQL 645
            E+ IVF+T+S SG  LFS+   GFD+V+IDEAAQA E+  L PLA G  +  LVGDP+QL
Sbjct: 794  EAEIVFTTVSSSGRKLFSRLTHGFDMVVIDEAAQASEVGVLPPLALGAARCVLVGDPQQL 853

Query: 646  PATVISTTAKKFGYDKSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYAESLEDAPD 705
            PATVIS  A    Y +SLF+RFQ AG P  +L +QYRMHP+IR FPSR FY   L+D+  
Sbjct: 854  PATVISKAAGTLLYSRSLFERFQLAGCPTLLLTVQYRMHPQIRDFPSRYFYQGRLKDSES 913

Query: 706  VKLRTTRAWHAFRCYGPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYHKLVISYPEL 759
            +       ++      P+ FF++  G+ES   GS S+ NVDEA F + +Y  L  +   L
Sbjct: 914  ISSAPDEIYYKDPVLRPYLFFNISHGRESHRGGSVSYENVDEARFCVGVYMHLQKTLKSL 973

BLAST of MC08g0843 vs. TAIR 10
Match: AT1G65810.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 184.5 bits (467), Expect = 3.5e-46
Identity = 109/301 (36.21%), Postives = 168/301 (55.81%), Query Frame = 0

Query: 457 IRSAILDESVIVFSTLSFSGSSLFSKWNRGFDVVIIDEAAQAVELATLVPL-ANGCKQVF 516
           ++   LD + ++F T S   SS     +    +++IDEAAQ  E  + +PL   G +   
Sbjct: 526 LQKLCLDNAYLLFCTAS---SSARLHMSSPIQLLVIDEAAQLKECESAIPLQLRGLQHAI 585

Query: 517 LVGDPEQLPATVISTTAKKFGYDKSLFKRFQTAGYPVTMLKIQYRMHPEIRSFPSREFYA 576
           L+GD +QLPA + S  A +    +SLF+R    G+   +L +QYRMHP I  FP+REFY 
Sbjct: 586 LIGDEKQLPAMIKSNIASEADLGRSLFERLVLLGHNKQLLNMQYRMHPSISIFPNREFYD 645

Query: 577 ESLEDAPDVKLRT-TRAWHAFRCYGPFCFFDLHEGKESQPPGSGSWVNVDEAEFVLLLYH 636
             + DAP V+LR+  + +   + YGP+ F ++  G+E    G  S  N+ E   V  +  
Sbjct: 646 MKILDAPSVRLRSYEKKFLPEKMYGPYSFINIAYGREQFGEGYSS-KNLVEVSVVAEIVS 705

Query: 637 KLVISYPELKSNSQVAIISPYSQQVKLLQEKFKDTFGLDPSGIVDITSVDGCQGREKDIA 696
           KL     +      V +ISPY  QV  +QE+  + +  + +  V + SVDG QG E+DI 
Sbjct: 706 KLYSVSRKTGRTISVGVISPYKAQVFAIQERIGEKYNTEGTFTVSVRSVDGFQGGEEDII 765

Query: 697 IFSCVRASENRSIGFLSDCRRMNVGITRARSSILVVGSASTLKRDEH-WNNLVESAQKRD 755
           I S VR++ N +IGFLS+ +R NV +TRAR  + ++G+ +TL  +   W  LV+ A+ R+
Sbjct: 766 IISTVRSNGNGAIGFLSNQQRTNVALTRARYCLWILGNEATLTNNRSVWRQLVDDAKARN 822