MC08g0808 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0808
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMethyltransferase
LocationMC08: 6588203 .. 6594408 (+)
RNA-Seq ExpressionMC08g0808
SyntenyMC08g0808
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTCATCCAAATTCCTTTCTTTCTTTTTAATTATCACGGGTACTCTTTCTAAATTAAATATCGTATATTTATGTAATTTTGGTTCCAAATTCATTTAATTAAATATGGATTTATACCATCTTAAGTTGATATTACTAAAAATTTGATTTCTATATTAGGTTCATAATTTATTCTTCAAATTAAAAAGTTTTTAGATTTTTTTTCAATGAATAATTGTTTTGAATTCTTTACTTTTAAAGTTAAAGATTTTTTTTTAAACATAATTGACATATATTTTCAAGTATAAATTGTGACTTCATCCCTCCATTCAAATTTAAAAATTTAAGATTTTTTTTTTCATATTTAGATTAAAATTGAGGAGGGAGCGGTGTTCCTATTTATTTCGAGACTCAACACAACTCATACTCATCCTTCTCCATCGGAATCTCAATAGGTGCCCTAACCCTAACCACCTCCGATTCCGATTTCTGACGACGGCGCGTTATTTTTTCAGGAGTCTGCTAAGAGATAAATGTCGAAGGACGGAGCGCTCGATCTCGCCTCCGGCGTCGGCGGAAAGATGAGCAAAGCCGACGTTCTCTCGGCCGTCGAGAAGTATCCTCTCCCTCTCTCTCTCTCTGCAAACTTTCTGTGCTTTCTCCGTTTCTCGTCTTCTCCCCCAAGTCTTTGACTCCGAGGGTTTCTTTTCTCCGTTTTGGTCCGGTTCTTTGGGTTCGGTCTGATCTCGCCAAGTTCTGTCTCTCGATATTCACAAAATTGGGGAAACTGAAGCCTAACGGAATCATTCGAATTGGGGAATTTTGAAAAAAAAAAGGGTCAAATTACATAAATTAACCATGATTTTACCATTTATTTAAAAAGTACACCTATCCATTTAACGGTTCAATATATTTTTTATATTTCATCAACGAAAATATTGAGCAAGAATCTAAAACCACGTGATAATTTCAATCACAACCAGAATATTATTAAATTAATATATTTTATGAAATTAACAACAATTTAATTGTCAGATCATTAATCAACTACCACTCACTCCAAAGCATCTTCAAGATTATTTAGAAATATTTTATAAAATTTAAGAATTAGAAATATATGTTTAAAACTTCGGTTATCAAATTGACACTCATTCTAAATCTTACCCGTTATTTGCTCCGAATTTGTGATGTGTTCTAATAGAATTTGAGAGATATTTAGCTGTGCTTTTTCCCATTTGTTTCTAAAACATGTTTTGTAAATTGATATAGAAAAATGGGTTTGTATTGTTTTTAATTTTGAAATTGGGTTTCTGAAAATAAACTTTATTATTTTGTTTTCATTTATTTTTAAATTATAAAAACACAATGGAGACAGTTTATAAACAATGGAAACAAAAAAACAAAACATTATCTATATTTTTAATTGTCTTTTATTGTAAAAACTTATTTTATTGTTTTTTTAAATAAGACTACACGTGTGTGTTGGTTGGAGCTGTATTATATTTAATAAATAAATAAATTAACTCATAAATATTTTTTAAAAGGAGTTCTGTAATATTTATAATTCACTGAAAAATACACCATATAGTTCCCAGAGCCAATGGTCGAGACAAGAATCGAAAAATAAATTTTTTTTAGTGGTCAATTTGAAAATACTTTGAAAAAGTTTAGTATTACATGAAAACCATACTCGGTTACCTTCCTCGCCTGCCACGTCAACATCATCGAGATTAAAAATAAAAAAGATTAAGGGAAGGGAAGGGTGTATGGTTTAATGGCGGGTAAATTAGGGTTTAGTTTTGAGGAACGTGGTGCAGGTACGAGAAATACCATGGGTTCTACGGAGGCGAAAAGGAGGACAGGGAAGCCAACTACAGGGACATGGTAAGAGGCAACTAATAATTTCCTGTTATTTGTTCTGATCGATCTTCGCCATATCTAATTATCTGTATTTTAATTTTTAGGTCAACAAATACTACGATCTCGTGACCAGCTTTTATGAATTCGGTTGGGGAGAGTGCTTCCATTTTGCTCCTAGGTACCATCTTCCTCCTCTCCTGCATTTTAATGAAAAATGCTTTTCTCCTGAGTGCCACTGAAAAGATAAGCTTTGTAGATGGAGTGGCGAGTCTTTCCAGGAGAGCATCAAGAGACACGAGCACTTCCTTGCTTTACAGTTGGGTTTGAAGCCTGGGCACAAGGTTCTGGATGTTGGATGTGGCATAGGCGGACCACTTAGAGAGATTGCAAGATTCAGGTGCTCTTAATTCTCATCATTGCCACGTATATATATATATATATATATTTTTTTATCCGTAAGTGTCTAGGTGAGTTTACGCGCACCTCAACTAATCTCAGTGTCTAGGTGAGTTTACGCGCACCTCAACTAATCTCACGGGCAACTGCCTGACCCTACCAAAAAACACATTAGAATTACTAATTCCTAAAGTAGATGGTCATCATGGAATTTGAACTCATTCTCTTTTGGGTCGGTGAGCCACTCATGATGGTTGATCCTTAAGATATTTTTAGTTCTTAAATGGATGCTATACGGTCTTGACTAGGAATTATTTTAAATGTCTTCTTATTTCTTATGGAAGAGGAGTCCTTTGCATTTTTTCTTCTTAAGAGACAATTTCATTGATGGCATGAAATTACAAAAACGAGAGAGATCTCCATTTAGGTAGTTACAAAATAAGTCCCCGATTGGGTAAAAGATTATAAAAACTATAGTGATGAAAAGGGGGAGCATTTACGCTATGATATAACTAATCAAATCAGACTATATCATAAAAAAAAAATAAAAAAAATTAGGAAGAGAATTCTCCTCGTCTTTAGAGATTGAATGATTCCTTTCTTGTCGAGTGTGATCTAGAAAAATGCTCTCTCAAAAGGGTGCCTTAGGGTCGGGAGGAAAAACCATGGACCAATTGAAAGCATCGAGAAGACTTAGAGAGGTGATAAGAATGCTATTAGGATAGAATTAGGGTATATTAGTAATTAGTTGGGGAAGTTGTTATAAATAGAGTGAGTGGAAAAGAGAGGAGACGGGTAATTTTTTAGTATGGTTTAGGCTTGAGTGATCTCAAGAGGGGAGGGTCCAAGTACCTCGAGTCACTTGAGAGTTATTGTGTGTTGTTCTATCTTCTATATTTCAATATATTTCTAGTTGTTCGGGTTCTATTAGATTTAAGGTCGGAATTTGCAAGAATGCTCATTTTTTGAGTGAACATTTCAATTTTCCAGCTCAACAATGTTTTTGACTAATGATTGTTAGGATTAATAGTAGGATGATCTCAAATTGTATTCTTGATACTGCATGTTAATGTTATTGTTAATAATATTTTACCTTCTCAGCATCTCATATTCTATGTAAATCTGTCACAGCTATACTTCAATTACTGGGTTGAACAACAACGGCTACCAGATTACAAGAGGAGAGGTAATTTTTGGCCACGACCATCAACTTTTTGTTCTCTCTTATTATTTTCCCATCTGTCTATTATTTAGTTTTGCCTTTTTTTTTTACTGAGTTCTTATCTGAAGTAGTACCTACATGTACTCTTCATTAACCATTTCACTGTTATGATCTTATTTTATTTCACAGGAACTGAACCGCATTGCAAAATTGGATGAGACTTGCAACTTTGTCAAGGTTTGTGTAGATTAAATTCAGCCTTTTGAAAATTTCTCGTGTCTCATGAGTTGGTTAAATCCTTCCTTGGTATAAATGGTCCCGATCACTAGATTTTGATTTCTGAATGCCATGAGAACCATTGCTTGCTTGATGGCAATAGAAATGGCTGAATTATGGGAATTTTCATGACATTAGAGTAACATTTTGAAAGTTTATTGACTGTGGTTTGTTCTTCTTCCGTATTTTATGAACATGGAATGATCAGTATTCCTCTACTAGAAACTCATAACCAAACTTCATATCTCGAACCTAGAACCGAGAAAGGCTAGATATGAGACCATATATGGCCTTTTATTTTTTTTTTTAATTTTTGTTTCACTGTTGGAGCAAATAGGATGATCCAACTAAGGGCTGCCCCCTCTTCCCTTAATATGAACTTCTTGACATTGTGCCAAAAAGCATAATGTAAAAGCTTATGTTAACAAATCACATGATTCGTTTCTTATACATTCTACAGGGTGACTTTATGAAAATGCCATTTGATGATAACTCGTTCGATGCAATATACGCGATCGAAGCCACTTGTCACGCACCAGATGCAGTAGGTCCTTATTGTATTTGTTAAGTATGAGTGTACAGCAATAATTTCTGGTTTAACTGCCTGTTTTGCCTCTGCCAGTATGGCTGCTACAAGGAGATCCACAGAGTATTAAAGCCTGGCCAACATTTTGCTGGTTATGAATGGTGCTTGACCGACTCGTTCAATCCTAATGACCAAGATCACCAAAGAATAAAGGTTATCTTGCTGATTATCTATCATGTCCCCCTTTTCCTCTGAATCTGATACAGATTATATTCACAATTTTCAATTTTATAGGCTGAAATTGAGGTTGGCAGTGGGCTTCCAGATATCAAGACGATAGGAAAGTGCATAGGAGCTTTGAAACAAGCAGGTTTTGAGGTGGATTGGAAAATGATCTTTCATTTGATTATATATTTTCTTTTCAGTTAAGATAAGATACTATTTTGATCCATGTAATTTAAATTTTGGTTCATTTTAGTCCTTGTTCTTTCAAAATGTTAGCTTTTGTTCTTATACTTTTGAAAAAGGATCATTTTGGTCTCTTAATTTTATTTTAAGCTCATTTATTTTGTCACAATTTCAACACAACACTATTTATACTAAACATATTCCATTCAATGTAACAAAGATTTTATATGAAAGAGTTTTTGTTACGTGTAGAATTTGTGTTAAAATTTTGAAAATAGGGACCAAAATTGTCACTTTTTATAAGTTTAGAGATCAAAACAAACCTTTTGAAAGTATAGGGGATGAAATGAACCAAATTTGGAAGCATAAGGACCAAATAAACCAAGATGAAAGTACCAAAATAGTATTTCAACCTTTCTTTCCACTTTTTCATGAATGGATAAAAGGTACCAGATCGGGTTTAAAGAAATGAGCTTATATATGTATATATATACTCTGCACTCTGTATAGGTTATATGGGAAAAGGACCTTGCTAAAGATTCACCTGTTCCTTGGTACTTGCCTTTAGATGGTGGCCAGTTCTCAATCACCAACTTCCGCGCTACAGCAATCGGGCGTTGCGTGACAAAGTACATGGTAAGTTGACTCGATTGTGATAAAGCTGAACCTCGACCTTGCTACCGTTGCATAATTTCGACTATGGATATGGAAGAAGCTGATGAATTGCTGGGTTTTTTCTTTCTGTAGGTGAGAGCTTTGGAGTATATCCGCCTCGCTCCAAAGGGTAGTGAAAGAGTTCAAAACTTTCTAGAGCAAGCAGCACAAGGGCTGGTTGAAGGTGGAAAGTGAGTATCATATAGCACAGTATTTTCATAAGATGGGATATTACATAGAAATTAGTAGGAACGCCTGAGAACTAAGCAAACCGAACCAATCCAGGTCGATCGGTTTGGTTGGACTGGTTTGACTTGTCAGAAATCAGAGTAGAAAAACTAATTCAGCAAGTTTGATCTACTCTTTAGTTGGTTTATAATCTGTCTCAAAGTTCTTATGAGAAACTTCAACACTAACTCACTCTTAGGAACATGATTTGCATTCATTTTTAGCTGCAGCACTCAGTTCTTTTTCTCATTAAACATGTACAACTCCCCAACGTGCTGATTATAATTCTACTTCGATGCTCCTACAGGAAAGAAGTCATGACCCCTATGTTCTTCTTCCTGGTGCAAAAACCGCTTTCGAGCAGCAAGTAAACCGACATTGGCGAGTAATTTGCTCCTATGGTTATATTATGATACGTAGTGTATTTTCATTTCTTACTGGCCTGAAAACCGTTAAGCGTGAGTAAGACAATATTAGTAAGTTATTAGTTACCATGGTTTTATGTAAGTTTAGCTGCAACTTTGATGAAACAAAGGTAGATTTGGTTTCAAGTCACATTTCTAGAGTTTAGGAAGTTTTCTTTAGTTGGATTACATTGGAATCATGTTCAAATGGTTTCCTTTGTTCCTTAAAGATCACTTGTGAAACGTTCTCAATCTCAGCTACTACTACAATTTGTCTTCTTCTGTTATCTGTAATGGCTAATTTGATGGGCTT

mRNA sequence

GATTCATCCAAATTCCTTTCTTTCTTTTTAATTATCACGGGAGTCTGCTAAGAGATAAATGTCGAAGGACGGAGCGCTCGATCTCGCCTCCGGCGTCGGCGGAAAGATGAGCAAAGCCGACGTTCTCTCGGCCGTCGAGAAGTACGAGAAATACCATGGGTTCTACGGAGGCGAAAAGGAGGACAGGGAAGCCAACTACAGGGACATGGTCAACAAATACTACGATCTCGTGACCAGCTTTTATGAATTCGGTTGGGGAGAGTGCTTCCATTTTGCTCCTAGATGGAGTGGCGAGTCTTTCCAGGAGAGCATCAAGAGACACGAGCACTTCCTTGCTTTACAGTTGGGTTTGAAGCCTGGGCACAAGGTTCTGGATGTTGGATGTGGCATAGGCGGACCACTTAGAGAGATTGCAAGATTCAGCTATACTTCAATTACTGGGTTGAACAACAACGGCTACCAGATTACAAGAGGAGAGGAACTGAACCGCATTGCAAAATTGGATGAGACTTGCAACTTTGTCAAGGGTGACTTTATGAAAATGCCATTTGATGATAACTCGTTCGATGCAATATACGCGATCGAAGCCACTTGTCACGCACCAGATGCATATGGCTGCTACAAGGAGATCCACAGAGTATTAAAGCCTGGCCAACATTTTGCTGGTTATGAATGGTGCTTGACCGACTCGTTCAATCCTAATGACCAAGATCACCAAAGAATAAAGGCTGAAATTGAGGTTGGCAGTGGGCTTCCAGATATCAAGACGATAGGAAAGTGCATAGGAGCTTTGAAACAAGCAGGTTTTGAGGTTATATGGGAAAAGGACCTTGCTAAAGATTCACCTGTTCCTTGGTACTTGCCTTTAGATGGTGGCCAGTTCTCAATCACCAACTTCCGCGCTACAGCAATCGGGCGTTGCGTGACAAAGTACATGGTGAGAGCTTTGGAGTATATCCGCCTCGCTCCAAAGGGTAGTGAAAGAGTTCAAAACTTTCTAGAGCAAGCAGCACAAGGGCTGGTTGAAGGTGGAAAGAAAGAAGTCATGACCCCTATGTTCTTCTTCCTGGTGCAAAAACCGCTTTCGAGCAGCAAGTAAACCGACATTGGCGAGTAATTTGCTCCTATGGTTATATTATGATACGTAGTGTATTTTCATTTCTTACTGGCCTGAAAACCGTTAAGCGTGAGTAAGACAATATTAGTAAGTTATTAGTTACCATGGTTTTATGTAAGTTTAGCTGCAACTTTGATGAAACAAAGGTAGATTTGGTTTCAAGTCACATTTCTAGAGTTTAGGAAGTTTTCTTTAGTTGGATTACATTGGAATCATGTTCAAATGGTTTCCTTTGTTCCTTAAAGATCACTTGTGAAACGTTCTCAATCTCAGCTACTACTACAATTTGTCTTCTTCTGTTATCTGTAATGGCTAATTTGATGGGCTT

Coding sequence (CDS)

ATGTCGAAGGACGGAGCGCTCGATCTCGCCTCCGGCGTCGGCGGAAAGATGAGCAAAGCCGACGTTCTCTCGGCCGTCGAGAAGTACGAGAAATACCATGGGTTCTACGGAGGCGAAAAGGAGGACAGGGAAGCCAACTACAGGGACATGGTCAACAAATACTACGATCTCGTGACCAGCTTTTATGAATTCGGTTGGGGAGAGTGCTTCCATTTTGCTCCTAGATGGAGTGGCGAGTCTTTCCAGGAGAGCATCAAGAGACACGAGCACTTCCTTGCTTTACAGTTGGGTTTGAAGCCTGGGCACAAGGTTCTGGATGTTGGATGTGGCATAGGCGGACCACTTAGAGAGATTGCAAGATTCAGCTATACTTCAATTACTGGGTTGAACAACAACGGCTACCAGATTACAAGAGGAGAGGAACTGAACCGCATTGCAAAATTGGATGAGACTTGCAACTTTGTCAAGGGTGACTTTATGAAAATGCCATTTGATGATAACTCGTTCGATGCAATATACGCGATCGAAGCCACTTGTCACGCACCAGATGCATATGGCTGCTACAAGGAGATCCACAGAGTATTAAAGCCTGGCCAACATTTTGCTGGTTATGAATGGTGCTTGACCGACTCGTTCAATCCTAATGACCAAGATCACCAAAGAATAAAGGCTGAAATTGAGGTTGGCAGTGGGCTTCCAGATATCAAGACGATAGGAAAGTGCATAGGAGCTTTGAAACAAGCAGGTTTTGAGGTTATATGGGAAAAGGACCTTGCTAAAGATTCACCTGTTCCTTGGTACTTGCCTTTAGATGGTGGCCAGTTCTCAATCACCAACTTCCGCGCTACAGCAATCGGGCGTTGCGTGACAAAGTACATGGTGAGAGCTTTGGAGTATATCCGCCTCGCTCCAAAGGGTAGTGAAAGAGTTCAAAACTTTCTAGAGCAAGCAGCACAAGGGCTGGTTGAAGGTGGAAAGAAAGAAGTCATGACCCCTATGTTCTTCTTCCTGGTGCAAAAACCGCTTTCGAGCAGCAAGTAA

Protein sequence

MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTSFYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCHAPDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGKCIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYIRLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK
Homology
BLAST of MC08g0808 vs. ExPASy Swiss-Prot
Match: Q9LM02 (Cycloartenol-C-24-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=SMT1 PE=1 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 5.2e-158
Identity = 251/335 (74.93%), Postives = 299/335 (89.25%), Query Frame = 0

Query: 7   LDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTSFYEFGW 66
           +DLAS +GGK+ K+DVL+AVEKYE+YH F+GG +E+R+ANY DMVNKYYDL TSFYE+GW
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60

Query: 67  GECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSYTSI 126
           GE FHFA RW GES +ESIKRHEHFLALQLG++PG KVLDVGCGIGGPLREIARFS + +
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120

Query: 127 TGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCHAPDAYG 186
           TGLNNN YQITRG+ELNR+A +D+TCNFVK DFMKMPF +NSFDA+YAIEATCHAPDAYG
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180

Query: 187 CYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGKCIGALK 246
           CYKEI+RVLKPGQ FA YEWC+TD+F+P++ +HQ+IK EIE+G GLPDI+   KC+ ALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240

Query: 247 QAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYIRLAPKG 306
           QAGFEVIWEKDLAKDSPVPWYLPLD   FS+++FR TA+GR +TK MV+ LEYIRLAP+G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300

Query: 307 SERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKP 342
           S+RV NFLEQAA+GLV+GG++E+ TPM+FFL +KP
Sbjct: 301 SQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 335

BLAST of MC08g0808 vs. ExPASy Swiss-Prot
Match: Q6ZIX2 (Cycloartenol-C-24-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Smt1-1 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.1e-155
Identity = 248/343 (72.30%), Postives = 302/343 (88.05%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MS+ GA+DLASG+GGK++K +V SAV++YEKYHG+YGG++E R++NY DMVNKYYDL TS
Sbjct: 1   MSRSGAMDLASGLGGKITKDEVKSAVDEYEKYHGYYGGKEEARKSNYTDMVNKYYDLATS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYE+GWGE FHFA RW+GES +ESIKRHEHFLALQLG+KPG KVLDVGCGIGGPLREIA+
Sbjct: 61  FYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQLGVKPGMKVLDVGCGIGGPLREIAK 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FS  S+TGLNNN YQITRG+ELNR+A +  TC+FVK DFMKMPF DN+FDA+YAIEATCH
Sbjct: 121 FSLASVTGLNNNEYQITRGKELNRVAGVSGTCDFVKADFMKMPFSDNTFDAVYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APD  GCYKEI+RVLKPGQ FA YEWC+TD + PN+  H+RIK EIE+G+GLPDI++  +
Sbjct: 181 APDPVGCYKEIYRVLKPGQCFAVYEWCITDHYEPNNATHKRIKDEIELGNGLPDIRSTQQ 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ A K AGFEVIW+KDLA+DSPVPWYLPLD  +FS+++FR T +GR +T+ MV+ALEY+
Sbjct: 241 CLQAAKDAGFEVIWDKDLAEDSPVPWYLPLDPSRFSLSSFRLTTVGRAITRTMVKALEYV 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLS 344
            LAP+GSERV NFLE+AA+GLVEGGKKE+ TPM+FFLV+KP+S
Sbjct: 301 GLAPQGSERVSNFLEKAAEGLVEGGKKEIFTPMYFFLVRKPIS 343

BLAST of MC08g0808 vs. ExPASy Swiss-Prot
Match: Q54I98 (Probable cycloartenol-C-24-methyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=smt1 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.1e-94
Identity = 162/299 (54.18%), Postives = 207/299 (69.23%), Query Frame = 0

Query: 43  REANYRDMVNKYYDLVTSFYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGH 102
           R+ NY  MVN +YDL T FYEFGWG+ FHFA R   ESF+ SI RHE ++A QLGL PG 
Sbjct: 54  RKNNYTHMVNTFYDLATDFYEFGWGQSFHFATRHKYESFEASIARHEMYMAHQLGLFPGM 113

Query: 103 KVLDVGCGIGGPLREIARFSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKM 162
           KV+D+GCG+GGP+R IARFS  ++ GLNNN YQI RG+ LN  A L   C+F+K DFM +
Sbjct: 114 KVIDIGCGVGGPMRTIARFSGANVVGLNNNEYQIQRGKRLNESAGLSHLCSFIKADFMHV 173

Query: 163 PFDDNSFDAIYAIEATCHAPDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRI 222
           P +DN++D  Y IEATCHAPD  G YKE+ R++KPG  F GYEW +T+ FNP D     I
Sbjct: 174 PVEDNTYDCAYQIEATCHAPDLVGLYKEVFRIVKPGGLFGGYEWIMTNKFNPEDPVEVNI 233

Query: 223 KAEIEVGSGLPDIKTIGKCIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRA 282
           K +IE+G+GLPD+    + I A K AGFEVI   D+A+ S +PWYLPL  G  SIT F  
Sbjct: 234 KKQIELGNGLPDLVKPAEIINAAKAAGFEVITAFDVAETSELPWYLPLSSG-VSITGFLH 293

Query: 283 TAIGRCVTKYMVRALEYIRLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKP 342
           T +GR +T    + LE ++LAP GS     +L+ AA  LV+GG+K++ +PMFF L +KP
Sbjct: 294 TGVGRYLTGKFTQLLEIVKLAPAGSYNTNVWLQNAATFLVQGGEKQIFSPMFFLLCRKP 351

BLAST of MC08g0808 vs. ExPASy Swiss-Prot
Match: Q759S7 (Sterol 24-C-methyltransferase OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=ERG6 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.0e-84
Identity = 157/336 (46.73%), Postives = 215/336 (63.99%), Query Frame = 0

Query: 15  GKMSKADVLSAVEKYEKYHGFYGGEKEDRE-ANYRDMVNKYYDLVTSFYEFGWGECFHFA 74
           GK  +A   +  +    + G    E E+R  A+Y +  + YY++VT FYE+GWG  FHF+
Sbjct: 34  GKNREAQQEAVAKYLRHWDGATDAEAEERRLADYNESTHSYYNVVTDFYEYGWGASFHFS 93

Query: 75  PRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSYTSITGLNNNG 134
             ++GESF  S+ RHEH+LA + G+  G  VLDVGCG+GGP REIARF+   + GLNNN 
Sbjct: 94  RFYTGESFAMSMARHEHYLAHRAGITSGDLVLDVGCGVGGPAREIARFTGCRVVGLNNND 153

Query: 135 YQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCHAPDAYGCYKEIHR 194
           YQI +G+  +R   L +  ++VKGDFM M F D +FD +YAIEATCHAP   G Y EI+R
Sbjct: 154 YQIMKGKHYSRKLGLGDQVSYVKGDFMNMDFPDATFDKVYAIEATCHAPSFEGVYGEIYR 213

Query: 195 VLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGKCIGALKQAGFEVI 254
           VLKPG  FA YEW +T++++  + +H+RI  +IE+G G+P + ++     AL + GFE++
Sbjct: 214 VLKPGGVFAVYEWVMTENYDETNPEHRRIAYDIELGDGIPKMYSVKVARDALAKVGFEIL 273

Query: 255 WEKDLA-KDSPVPWYLPLDGGQFSI-------TNFRATAIGRCVTKYMVRALEYIRLAPK 314
            ++D A  D PVPWY PL G    I       T  R +A+GR VT   V  +E +RLAP+
Sbjct: 274 VDEDRADNDDPVPWYYPLTGEWAYIQTIADLGTFVRTSALGRRVTSAFVSLMETLRLAPR 333

Query: 315 GSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKP 342
           GS  V N LE AA GLV GGK ++ TPM  F+ +KP
Sbjct: 334 GSSEVVNALESAAAGLVAGGKSKLFTPMMLFVARKP 369

BLAST of MC08g0808 vs. ExPASy Swiss-Prot
Match: P25087 (Sterol 24-C-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ERG6 PE=1 SV=4)

HSP 1 Score: 315.1 bits (806), Expect = 1.0e-84
Identity = 160/343 (46.65%), Postives = 219/343 (63.85%), Query Frame = 0

Query: 11  SGVGGKMSKADVL--SAVEKY-EKYHGFYGGEKEDRE-ANYRDMVNKYYDLVTSFYEFGW 70
           +G+   MSK +     AV+KY   + G    + E+R   +Y +  + YY++VT FYE+GW
Sbjct: 26  TGLSALMSKNNSAQKEAVQKYLRNWDGRTDKDAEERRLEDYNEATHSYYNVVTDFYEYGW 85

Query: 71  GECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSYTSI 130
           G  FHF+  + GESF  SI RHEH+LA + G++ G  VLDVGCG+GGP REIARF+  ++
Sbjct: 86  GSSFHFSRFYKGESFAASIARHEHYLAYKAGIQRGDLVLDVGCGVGGPAREIARFTGCNV 145

Query: 131 TGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCHAPDAYG 190
            GLNNN YQI + +   +   L +  +FVKGDFMKM F++N+FD +YAIEATCHAP   G
Sbjct: 146 IGLNNNDYQIAKAKYYAKKYNLSDQMDFVKGDFMKMDFEENTFDKVYAIEATCHAPKLEG 205

Query: 191 CYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGKCIGALK 250
            Y EI++VLKPG  FA YEW +TD ++ N+ +H++I  EIE+G G+P +  +     ALK
Sbjct: 206 VYSEIYKVLKPGGTFAVYEWVMTDKYDENNPEHRKIAYEIELGDGIPKMFHVDVARKALK 265

Query: 251 QAGFEVIWEKDLA-KDSPVPWYLPLDGGQFSITN-------FRATAIGRCVTKYMVRALE 310
             GFEV+  +DLA  D  +PWY PL G    + N       FR + +GR  T  MV  +E
Sbjct: 266 NCGFEVLVSEDLADNDDEIPWYYPLTGEWKYVQNLANLATFFRTSYLGRQFTTAMVTVME 325

Query: 311 YIRLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKP 342
            + LAP+GS+ V   LE AA GLV GGK ++ TPM  F+ +KP
Sbjct: 326 KLGLAPEGSKEVTAALENAAVGLVAGGKSKLFTPMMLFVARKP 368

BLAST of MC08g0808 vs. NCBI nr
Match: XP_022152711.1 (cycloartenol-C-24-methyltransferase-like [Momordica charantia])

HSP 1 Score: 720 bits (1858), Expect = 3.02e-262
Identity = 345/346 (99.71%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS
Sbjct: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR
Sbjct: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK
Sbjct: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLS SK
Sbjct: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSRSK 346

BLAST of MC08g0808 vs. NCBI nr
Match: XP_038904749.1 (cycloartenol-C-24-methyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 674 bits (1738), Expect = 5.95e-244
Identity = 319/346 (92.20%), Postives = 337/346 (97.40%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSK+GA DLASGVGGKMSKADVL AVEKYEKYHGFYGGEK++REANY DMVNKYYDLVTS
Sbjct: 1   MSKEGAFDLASGVGGKMSKADVLCAVEKYEKYHGFYGGEKKEREANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGECFHFAPRW+GESF+ESIK+HEHFLALQLGLKPG KVLDVGCGIGGPLREIAR
Sbjct: 61  FYEFGWGECFHFAPRWTGESFRESIKKHEHFLALQLGLKPGQKVLDVGCGIGGPLREIAR 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNN YQITRGEELNRIAKLD+TCNFVKGDFMKMPFDDNSFDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNSYQITRGEELNRIAKLDKTCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APDAYGC+KEI+RVLKPGQHFA YEWCLT+SF+PN+QDHQRIKAEIE+GSGLPDIKTIGK
Sbjct: 181 APDAYGCFKEIYRVLKPGQHFAAYEWCLTNSFDPNNQDHQRIKAEIEIGSGLPDIKTIGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ ALKQAGFEVIW KDLA+DSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CLEALKQAGFEVIWGKDLAEDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPM+FFLV+KPL SS+
Sbjct: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMYFFLVRKPLLSSE 346

BLAST of MC08g0808 vs. NCBI nr
Match: XP_022982721.1 (cycloartenol-C-24-methyltransferase-like [Cucurbita maxima] >XP_022982722.1 cycloartenol-C-24-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 671 bits (1731), Expect = 6.94e-243
Identity = 318/346 (91.91%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSKDGA DLASGVGGKMSKADV+ AVEKYEKYHGFYGGEKE+REANY DMVNKYYDLVTS
Sbjct: 1   MSKDGAFDLASGVGGKMSKADVICAVEKYEKYHGFYGGEKEEREANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGECFHFAPR +GESF+ESIKRHEHFLALQLGLKPG KVLDVGCGIGGPLREIAR
Sbjct: 61  FYEFGWGECFHFAPRLTGESFRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIAR 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNN YQITRGEELNRIAKLD+TCNFVKGDFMKMPF+DN+FDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNSYQITRGEELNRIAKLDKTCNFVKGDFMKMPFEDNTFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APDAYGC+KEI+RVLKPGQHFA YEWCLT+SFNPN+QDHQRIKAEIE+GSGLPDIKTIGK
Sbjct: 181 APDAYGCFKEIYRVLKPGQHFAAYEWCLTNSFNPNNQDHQRIKAEIEIGSGLPDIKTIGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ ALK+AGFEVIW KDLA+DSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CLEALKEAGFEVIWGKDLAEDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPM+FFLV+KPLSS +
Sbjct: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMYFFLVRKPLSSGE 346

BLAST of MC08g0808 vs. NCBI nr
Match: XP_022934887.1 (cycloartenol-C-24-methyltransferase-like [Cucurbita moschata] >XP_022934888.1 cycloartenol-C-24-methyltransferase-like [Cucurbita moschata] >XP_023526271.1 cycloartenol-C-24-methyltransferase-like [Cucurbita pepo subsp. pepo] >XP_023526272.1 cycloartenol-C-24-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 670 bits (1728), Expect = 1.99e-242
Identity = 317/346 (91.62%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSKDGA DLASGVGGKMSKADV+ AVEKYEKYHGFYGGEKE+REANY DMVNKYYDLVTS
Sbjct: 1   MSKDGAFDLASGVGGKMSKADVICAVEKYEKYHGFYGGEKEEREANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGECFHFAPR +GESF+ESIKRHEHFLALQLGLKPG KVLDVGCGIGGPLREIA+
Sbjct: 61  FYEFGWGECFHFAPRLTGESFRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIAK 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNN YQITRGEELNRIAKLD+TCNFVKGDFMKMPF+DN+FDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNSYQITRGEELNRIAKLDKTCNFVKGDFMKMPFEDNTFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APDAYGC+KEI+RVLKPGQHFA YEWCLT+SFNPN+QDHQRIKAEIE+GSGLPDIKTIGK
Sbjct: 181 APDAYGCFKEIYRVLKPGQHFAAYEWCLTNSFNPNNQDHQRIKAEIEIGSGLPDIKTIGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ ALK+AGFEVIW KDLA+DSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CLEALKEAGFEVIWGKDLAEDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPM+FFLV+KPLSS +
Sbjct: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMYFFLVRKPLSSGE 346

BLAST of MC08g0808 vs. NCBI nr
Match: XP_011651767.1 (cycloartenol-C-24-methyltransferase isoform X1 [Cucumis sativus] >KAE8650928.1 hypothetical protein Csa_001148 [Cucumis sativus])

HSP 1 Score: 662 bits (1707), Expect = 3.16e-239
Identity = 311/346 (89.88%), Postives = 333/346 (96.24%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSK+GA DLASGVGGKMSKADVL AVEKYEKYHG+YGGEKE+REANY DMVNKYYDLVTS
Sbjct: 1   MSKEGAFDLASGVGGKMSKADVLCAVEKYEKYHGYYGGEKEEREANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGE FHFAPRW GESF+ESIKRHEHFLAL+LGLKPG KVLDVGCGIGGPLREIA+
Sbjct: 61  FYEFGWGESFHFAPRWIGESFRESIKRHEHFLALELGLKPGQKVLDVGCGIGGPLREIAK 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNN YQITRGEELNRIAKLD+TCNFVKGDFMKMPFDDN+FDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNSYQITRGEELNRIAKLDKTCNFVKGDFMKMPFDDNTFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APD YGC+KEI+RVLKPGQ FA YEWCLT+SF+PN+QDHQRIKAEIE+GSGLPDIKTIGK
Sbjct: 181 APDPYGCFKEIYRVLKPGQRFAAYEWCLTNSFDPNNQDHQRIKAEIEIGSGLPDIKTIGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ ALKQAGFE++WEKDL +DSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CLEALKQAGFEIVWEKDLTEDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPM+FF+V+KPLSS +
Sbjct: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMYFFVVRKPLSSGE 346

BLAST of MC08g0808 vs. ExPASy TrEMBL
Match: A0A6J1DIK1 (Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020366 PE=3 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 1.46e-262
Identity = 345/346 (99.71%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS
Sbjct: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR
Sbjct: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK
Sbjct: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLS SK
Sbjct: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSRSK 346

BLAST of MC08g0808 vs. ExPASy TrEMBL
Match: A0A6J1IXC3 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481505 PE=3 SV=1)

HSP 1 Score: 671 bits (1731), Expect = 3.36e-243
Identity = 318/346 (91.91%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSKDGA DLASGVGGKMSKADV+ AVEKYEKYHGFYGGEKE+REANY DMVNKYYDLVTS
Sbjct: 1   MSKDGAFDLASGVGGKMSKADVICAVEKYEKYHGFYGGEKEEREANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGECFHFAPR +GESF+ESIKRHEHFLALQLGLKPG KVLDVGCGIGGPLREIAR
Sbjct: 61  FYEFGWGECFHFAPRLTGESFRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIAR 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNN YQITRGEELNRIAKLD+TCNFVKGDFMKMPF+DN+FDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNSYQITRGEELNRIAKLDKTCNFVKGDFMKMPFEDNTFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APDAYGC+KEI+RVLKPGQHFA YEWCLT+SFNPN+QDHQRIKAEIE+GSGLPDIKTIGK
Sbjct: 181 APDAYGCFKEIYRVLKPGQHFAAYEWCLTNSFNPNNQDHQRIKAEIEIGSGLPDIKTIGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ ALK+AGFEVIW KDLA+DSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CLEALKEAGFEVIWGKDLAEDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPM+FFLV+KPLSS +
Sbjct: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMYFFLVRKPLSSGE 346

BLAST of MC08g0808 vs. ExPASy TrEMBL
Match: A0A6J1F901 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441927 PE=3 SV=1)

HSP 1 Score: 670 bits (1728), Expect = 9.63e-243
Identity = 317/346 (91.62%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSKDGA DLASGVGGKMSKADV+ AVEKYEKYHGFYGGEKE+REANY DMVNKYYDLVTS
Sbjct: 1   MSKDGAFDLASGVGGKMSKADVICAVEKYEKYHGFYGGEKEEREANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGECFHFAPR +GESF+ESIKRHEHFLALQLGLKPG KVLDVGCGIGGPLREIA+
Sbjct: 61  FYEFGWGECFHFAPRLTGESFRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIAK 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNN YQITRGEELNRIAKLD+TCNFVKGDFMKMPF+DN+FDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNSYQITRGEELNRIAKLDKTCNFVKGDFMKMPFEDNTFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APDAYGC+KEI+RVLKPGQHFA YEWCLT+SFNPN+QDHQRIKAEIE+GSGLPDIKTIGK
Sbjct: 181 APDAYGCFKEIYRVLKPGQHFAAYEWCLTNSFNPNNQDHQRIKAEIEIGSGLPDIKTIGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ ALK+AGFEVIW KDLA+DSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CLEALKEAGFEVIWGKDLAEDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPM+FFLV+KPLSS +
Sbjct: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMYFFLVRKPLSSGE 346

BLAST of MC08g0808 vs. ExPASy TrEMBL
Match: A0A1S3CGW7 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103500325 PE=3 SV=1)

HSP 1 Score: 655 bits (1689), Expect = 8.47e-237
Identity = 309/346 (89.31%), Postives = 331/346 (95.66%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSK+GA DLASGVGGKMSKADVL AVEKYEKYHGFYGGE+E+REANY DMVNKYYDLVTS
Sbjct: 1   MSKEGAFDLASGVGGKMSKADVLCAVEKYEKYHGFYGGEREEREANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGE FHFAPRW GESF+ESIKRHEHFLAL+LGLKPG KVLDVGCGIGGPLREIA+
Sbjct: 61  FYEFGWGESFHFAPRWIGESFRESIKRHEHFLALELGLKPGQKVLDVGCGIGGPLREIAK 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTSITGLNNN YQITRGEELNRIAKLD+TCNFVKGDFMKMPFDDN+FDAIYAIEATCH
Sbjct: 121 FSYTSITGLNNNSYQITRGEELNRIAKLDKTCNFVKGDFMKMPFDDNTFDAIYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APD YGC+KEI+RVLKPGQ FA YEWCLT+SF+PN+QDHQ+IKAEIE+GSGLPDIKTI K
Sbjct: 181 APDPYGCFKEIYRVLKPGQRFAAYEWCLTNSFDPNNQDHQKIKAEIEIGSGLPDIKTIEK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ ALKQAGFE+IW KDL +DSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI
Sbjct: 241 CLEALKQAGFEIIWGKDLTEDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGSERVQNFLEQAA+GLVEGGKKEVMTPM+FFLV+KPLSS +
Sbjct: 301 RLAPKGSERVQNFLEQAARGLVEGGKKEVMTPMYFFLVRKPLSSDE 346

BLAST of MC08g0808 vs. ExPASy TrEMBL
Match: A0A1S3CFL1 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103500326 PE=3 SV=1)

HSP 1 Score: 595 bits (1534), Expect = 3.52e-213
Identity = 273/346 (78.90%), Postives = 318/346 (91.91%), Query Frame = 0

Query: 1   MSKDGALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTS 60
           MSK GALDLASG+GGK+ K DVLSAVEKYEKYH  YGGE+E+R+ANY DMVNKYYDLVTS
Sbjct: 1   MSKTGALDLASGLGGKLEKNDVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 61  FYEFGWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120
           FYEFGWGE FHFAPRW GES +ESIKRHEHFLALQL LKPG+KVLDVGCGIGGPLREIAR
Sbjct: 61  FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIAR 120

Query: 121 FSYTSITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCH 180
           FSYTS+TGLNNN YQI+RG+ELNR+AK+D+TC+FVK DFMKMPF DNSFDA+YAIEATCH
Sbjct: 121 FSYTSVTGLNNNEYQISRGKELNRVAKVDKTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 181 APDAYGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGK 240
           APDAYGCYKEI+RVLKPGQHFA YEWC+TD+F+PN+Q+HQ+IKAEIE+G GLPDI+  GK
Sbjct: 181 APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTGK 240

Query: 241 CIGALKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYI 300
           C+ ALKQAGFEV+WEKDLA +SP+PWYLPLD   FS+++FR TA+GR +TK MV+ LE+I
Sbjct: 241 CLEALKQAGFEVVWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKVLEFI 300

Query: 301 RLAPKGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 346
           RLAPKGS+RVQ+FLE+AA+GLVEGGKKE+ TPM+FFL +KPLS+++
Sbjct: 301 RLAPKGSQRVQDFLEKAAEGLVEGGKKEIFTPMYFFLARKPLSATE 346

BLAST of MC08g0808 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 558.5 bits (1438), Expect = 3.7e-159
Identity = 251/335 (74.93%), Postives = 299/335 (89.25%), Query Frame = 0

Query: 7   LDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTSFYEFGW 66
           +DLAS +GGK+ K+DVL+AVEKYE+YH F+GG +E+R+ANY DMVNKYYDL TSFYE+GW
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60

Query: 67  GECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSYTSI 126
           GE FHFA RW GES +ESIKRHEHFLALQLG++PG KVLDVGCGIGGPLREIARFS + +
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120

Query: 127 TGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCHAPDAYG 186
           TGLNNN YQITRG+ELNR+A +D+TCNFVK DFMKMPF +NSFDA+YAIEATCHAPDAYG
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180

Query: 187 CYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGKCIGALK 246
           CYKEI+RVLKPGQ FA YEWC+TD+F+P++ +HQ+IK EIE+G GLPDI+   KC+ ALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240

Query: 247 QAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYIRLAPKG 306
           QAGFEVIWEKDLAKDSPVPWYLPLD   FS+++FR TA+GR +TK MV+ LEYIRLAP+G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300

Query: 307 SERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKP 342
           S+RV NFLEQAA+GLV+GG++E+ TPM+FFL +KP
Sbjct: 301 SQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 335

BLAST of MC08g0808 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 558.5 bits (1438), Expect = 3.7e-159
Identity = 251/335 (74.93%), Postives = 299/335 (89.25%), Query Frame = 0

Query: 7   LDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREANYRDMVNKYYDLVTSFYEFGW 66
           +DLAS +GGK+ K+DVL+AVEKYE+YH F+GG +E+R+ANY DMVNKYYDL TSFYE+GW
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60

Query: 67  GECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSYTSI 126
           GE FHFA RW GES +ESIKRHEHFLALQLG++PG KVLDVGCGIGGPLREIARFS + +
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120

Query: 127 TGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCHAPDAYG 186
           TGLNNN YQITRG+ELNR+A +D+TCNFVK DFMKMPF +NSFDA+YAIEATCHAPDAYG
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180

Query: 187 CYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGKCIGALK 246
           CYKEI+RVLKPGQ FA YEWC+TD+F+P++ +HQ+IK EIE+G GLPDI+   KC+ ALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240

Query: 247 QAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYIRLAPKG 306
           QAGFEVIWEKDLAKDSPVPWYLPLD   FS+++FR TA+GR +TK MV+ LEYIRLAP+G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300

Query: 307 SERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKP 342
           S+RV NFLEQAA+GLV+GG++E+ TPM+FFL +KP
Sbjct: 301 SQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 335

BLAST of MC08g0808 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 254.6 bits (649), Expect = 1.1e-67
Identity = 131/334 (39.22%), Postives = 187/334 (55.99%), Query Frame = 0

Query: 14  GGKMSKADVLSAVEKYEKYHGFYGGEKEDREA-NYRDMVNKYYDLVTSFYEFGWGECFHF 73
           GG +S   V    + Y +Y  F+   KE   A    D V+ +Y+LVT  YE+GWG+ FHF
Sbjct: 39  GGSISAEKV---KDNYNQYWSFFRKPKEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHF 98

Query: 74  APRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSYTSITGLNNN 133
           +P   G+S +++ + HE      + +KPG K+LD GCG+GGP+R IA  S   +TG+  N
Sbjct: 99  SPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITIN 158

Query: 134 GYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCHAPDAYGCYKEIH 193
            YQ+ R +  N+ A LD  CN V G+F+KMPFD+N+FD  Y+IEATCHAP     Y EI 
Sbjct: 159 EYQVQRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIF 218

Query: 194 RVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGKCIGALKQAGFEV 253
           RV+KPG  F  YEW  T+ +  +D++H+ +   IE G  LP +++        K+ GFEV
Sbjct: 219 RVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEV 278

Query: 254 IWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYIRLAPKGSERVQN 313
           + EKDLAK    PW+  L  G+ +                +V  L  I +APKG+  V  
Sbjct: 279 VKEKDLAKPPSKPWWNRLKMGRIAYWR----------NHVVVVILSAIGVAPKGTVDVHK 338

Query: 314 FLEQAAQGLVEGGKKEVMTPMFFFLVQKPLSSSK 347
            L + A  L  GG+  + +PM   L +KP  +S+
Sbjct: 339 MLFKTADYLTRGGETGIFSPMHMILCRKPEKASE 359

BLAST of MC08g0808 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 252.3 bits (643), Expect = 5.7e-67
Identity = 132/339 (38.94%), Postives = 187/339 (55.16%), Query Frame = 0

Query: 6   ALDLASGVGGKMSKADVLSAVEKYEKYHGFYGGEKEDREA-NYRDMVNKYYDLVTSFYEF 65
           A+DL+   GG +S   V    + Y++Y  F+   KE   A    D V+ +Y+LVT  YE+
Sbjct: 34  AVDLS---GGSISAEKV---QDNYKQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEW 93

Query: 66  GWGECFHFAPRWSGESFQESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSYT 125
           GWG+ FHF+P   G+S +++ + HE      + +KPG K+LDVGCG+GGP+R IA  S  
Sbjct: 94  GWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRA 153

Query: 126 SITGLNNNGYQITRGEELNRIAKLDETCNFVKGDFMKMPFDDNSFDAIYAIEATCHAPDA 185
           ++ G+  N YQ+ R    N+ A LD  C  V G+F++MPFDDNSFD  Y+IEATCHAP  
Sbjct: 154 NVVGITINEYQVNRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKL 213

Query: 186 YGCYKEIHRVLKPGQHFAGYEWCLTDSFNPNDQDHQRIKAEIEVGSGLPDIKTIGKCIGA 245
              Y EI+RVLKPG  +  YEW  T+ F   D +H  +   IE G  LP ++        
Sbjct: 214 EEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAET 273

Query: 246 LKQAGFEVIWEKDLAKDSPVPWYLPLDGGQFSITNFRATAIGRCVTKYMVRALEYIRLAP 305
            K+ GFE++ EKDLA     PW+  L  G+ +                +V+ L  + +AP
Sbjct: 274 AKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAYWR----------NHIVVQILSAVGVAP 333

Query: 306 KGSERVQNFLEQAAQGLVEGGKKEVMTPMFFFLVQKPLS 344
           KG+  V   L + A  L  GG+  + +PM   L +KP S
Sbjct: 334 KGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPES 356

BLAST of MC08g0808 vs. TAIR 10
Match: AT1G64970.1 (gamma-tocopherol methyltransferase )

HSP 1 Score: 72.4 bits (176), Expect = 8.0e-13
Identity = 47/173 (27.17%), Postives = 72/173 (41.62%), Query Frame = 0

Query: 48  RDMVNKYYDLVTSFYEFGWGECFH--FAPRWSGESFQESIKRHEHFLALQLGL------- 107
           R  + ++Y+  +  +E  WG+  H  F    S     +S  +      ++  L       
Sbjct: 61  RKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEESLRFAGVTD 120

Query: 108 ----KPGHKVLDVGCGIGGPLREIARFSYTSITGLNNNGYQITRGEELNRIAKLDETCNF 167
               K   KV+DVGCGIGG  R +A        G+  +  Q  R  +L     L    +F
Sbjct: 121 EEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQSLAHKASF 180

Query: 168 VKGDFMKMPFDDNSFDAIYAIEATCHAPDAYGCYKEIHRVLKPGQHFAGYEWC 208
              D +  PF+D  FD ++++E+  H PD     KE+ RV  PG       WC
Sbjct: 181 QVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVTWC 233

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LM025.2e-15874.93Cycloartenol-C-24-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=SMT1 PE=1... [more]
Q6ZIX21.1e-15572.30Cycloartenol-C-24-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q54I983.1e-9454.18Probable cycloartenol-C-24-methyltransferase 1 OS=Dictyostelium discoideum OX=44... [more]
Q759S71.0e-8446.73Sterol 24-C-methyltransferase OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51... [more]
P250871.0e-8446.65Sterol 24-C-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / ... [more]
Match NameE-valueIdentityDescription
XP_022152711.13.02e-26299.71cycloartenol-C-24-methyltransferase-like [Momordica charantia][more]
XP_038904749.15.95e-24492.20cycloartenol-C-24-methyltransferase-like isoform X1 [Benincasa hispida][more]
XP_022982721.16.94e-24391.91cycloartenol-C-24-methyltransferase-like [Cucurbita maxima] >XP_022982722.1 cycl... [more]
XP_022934887.11.99e-24291.62cycloartenol-C-24-methyltransferase-like [Cucurbita moschata] >XP_022934888.1 cy... [more]
XP_011651767.13.16e-23989.88cycloartenol-C-24-methyltransferase isoform X1 [Cucumis sativus] >KAE8650928.1 h... [more]
Match NameE-valueIdentityDescription
A0A6J1DIK11.46e-26299.71Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020366 PE=3 SV=1[more]
A0A6J1IXC33.36e-24391.91Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481505 PE=3 SV=1[more]
A0A6J1F9019.63e-24391.62Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441927 PE=3 SV=1[more]
A0A1S3CGW78.47e-23789.31Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103500325 PE=3 SV=1[more]
A0A1S3CFL13.52e-21378.90Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103500326 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G13710.13.7e-15974.93sterol methyltransferase 1 [more]
AT5G13710.23.7e-15974.93sterol methyltransferase 1 [more]
AT1G76090.11.1e-6739.22sterol methyltransferase 3 [more]
AT1G20330.15.7e-6738.94sterol methyltransferase 2 [more]
AT1G64970.18.0e-1327.17gamma-tocopherol methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013705Sterol methyltransferase C-terminalPFAMPF08498Sterol_MT_Ccoord: 278..341
e-value: 1.4E-24
score: 86.0
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 105..201
e-value: 2.4E-19
score: 69.8
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 26..279
e-value: 1.3E-46
score: 161.0
NoneNo IPR availablePIRSRPIRSR018249-2PIRSR018249-2coord: 103..198
e-value: 8.8E-6
score: 23.1
NoneNo IPR availablePIRSRPIRSR006325-1PIRSR006325-1coord: 99..169
e-value: 0.014
score: 12.8
NoneNo IPR availablePANTHERPTHR44068ZGC:194242coord: 1..343
NoneNo IPR availablePANTHERPTHR44068:SF1CYCLOARTENOL-C-24-METHYLTRANSFERASEcoord: 1..343
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 103..198
e-value: 5.52239E-14
score: 65.529
IPR030384SAM-dependent methyltransferase SMT-typePROSITEPS51685SAM_MT_ERG6_SMTcoord: 54..341
score: 104.625137
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 46..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0808.1MC08g0808.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0032259 methylation
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0006694 steroid biosynthetic process
molecular_function GO:0003838 sterol 24-C-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity