MC08g0735 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0735
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDiphthine methyl ester synthase
LocationMC08: 5996930 .. 5999427 (-)
RNA-Seq ExpressionMC08g0735
SyntenyMC08g0735
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GACAATTTTGTTTAAATTTGGTATGCCACGCAAGTTGGAAGTTAAGAAGTATCATGAGATAGACCTAAAATTTTCTGAGCATTATTCATAGAATAAGTTTGAATAGAGGTATATTTTGCAAATAAATTTCAATTTCAAATTCAGCATTATTATTAAAAAAAAAAAAAAATCCCAAATTTTACTGGTTTACAGTAGATGGACCAAAGCCCAGAGCATGATGGGTCAAAACCCACGATCAGGTAAAACGACGCCGCTTTTGCAAAATCCAGCATGATGTGAGCATATACGAAGAGTTCCGCTCCGTGAAAGCAGTGGCGCCGGCGCGTACGTTAGTCTAGGGTTTTAGCGAACGGTTAGATTTGGAAAGAATTAGGGTTTAAGGCAGAGCGAGAAGAGAAGAAGAAGAGTAGAAAGAACGATGCTGTACATTATAGGTTTAGGATTGGGCGACGAAAAGGACATCACTCTCAGAGGCTTAGAATGTGTGAAGAAATGCAACAAGATTTACATGGAAGCTTACACTTCTCTCTTGTCCTTTGGCCTTGCTTCCGATGGAATTTCTTCGCTCGAGAAGCTCTACGGAAAGACCATCACAGTTGCGGATCGGGAAATGGTCGAGGAGAAGGCTGATGAGATGCTCTCCAAGGCTCGTACCTCTGATGTTGCCTTTTTGGTTGTTGGCGACGCTTTCGGGTATGAAAATTGCATTATCCTCTGCTTTTGAAGCTTCGATTTCTCTGTCGCGGATTCACCATTCTAGATTGTTTAATTCTGTGATTTGTGACTCTAAACATGCATCTTAACTTGAAGTTAAATGGCATAATTGAAAGTTTTTAAAATTGCCTTGTAATTGGGGCTGCGTGGAAGCCTTTTTTTTTTTCCTACTCTGAAGATGTTTGCCATTGGACTTACTTTTTGAGCAATATTTATGTTCTTCTATATACCGTGTCGTAAGGCAGAGCTACGACCCATTCGGATCTGGTCGTTCGAGCAAAGAGTTTGGGGATTGAAGTCAGAGTGGTGTACAATGCTTCTGTGATGAATGCAATTGGAATTTGTGGGTTGCAACTGTACCGCTATGGTGAGACTGTTTCGATACCGTTTTTTACTGAAACGTGGAAACCGAGTAGCTTTTATGAGAAGATTCAAAGAAACCGTGGACTTGGATTGCACACACTCTGCTTATTAGGTGGGATTCTGTTCTTTACTGTCTAAGCTGATAGTAAATCAGCTCTTGAATCTGAGGATTTTGTTATTTTGGTGAGGTTGAGAGATAAAAACACATTCTTTAACCCAACTGGTTTTTTTCATCAGTTGAGTCGTCGCTAATAGATAAATTTTGTTTAATGTCTTAATTGTTATATATCCATTTCATCGACACATCTGGTTAATACATTATATTCTGTCAGATATACGAGTAAAGGAACCGTCTTTGGAGTCTTTATGCAGGTTCGTATCCGGATCCCCTTATTTGTGTTTCAGATAACAACTTATGACGATTTTTGCAAGTTCTAGTATTCTTATTTTCCCCCTGAAGTCTCTTGTTTTATTTAGTTCAGTCATTCTGCTATGATAGAAGGAACTTAAAAGTATATGTCTGCTTTACATAAATTTTTCAGGGGAAGAAAAGAGTATGAACCACCTAGATTTATGTCTATAAACACAGCAATCGAGCAGCTTTTGGAGGTTGAGCAAATGGAAGGACAATCTGGTAATTCACATCAATGGCCTGATTTATTTATTTGTTTGTTTAAGATTCTATGTGCTTGATCAAGAGAACCTCTTTTTTTCAACATAGAGAATGCACTCCCAGAAGTTCATTGGTAGTAAGCGTGTCTGAGTGTTTAGAGATACCTATCAATGTCTTAGGTTCAAATCGTAAGTTAAAAATTAAGAACTTAAAACTTTTATTATCTGCTGGAGTTGACACTATGGACATCGAAAAGAAAAAGAAAAAGCATAAAGAATGCAGAAAAAAGACCAAATGTCGTTTGCTGTGTATACGAGGATTGCTTTTGTAATTAGCTTTTATGCTGCAGTATACAACGAAGACTCACTGTGTGTGGGATTTGCTCGTCTCGGAAGCGAAGATCAGATGATAGTAGCGGGCACCATGAAGCAGCTGAGAAGCATTGATTTCGGGCCGCCTCTGCATTGCCTTGTGATTGTAGGAAAGACCCATCCTGTTGAAGAAGAAATGTTGGACTTTTTTAAATCTGGAAATGAGAATCTTGAACAGGATGTCATTGACAGAACTACATAATTATTATCTGAGAGCCGAGATCCTTTCATTTACAGGTTATCCAAGTCCCGTTGTACCATTGCTTTTGAAAACTTTGAAATCTGCTTTACTTATATTTTAAAGTTATTTATATATATTATAGAACTCTTGGATTTCTTGGTTTTGACTGCTCAAAAATCAGTTATCTTAAACATTACAGCTGATTAGAGATAAATTTCAGAAATATTTCTGCAAATCATTGAATGGTTTATGGAAC

mRNA sequence

GACAATTTTGTTTAAATTTGGTATGCCACGCAAGTTGGAAGTTAAGAAGTATCATGAGATAGACCTAAAATTTTCTGAGCATTATTCATAGAATAAGTTTGAATAGAGGTATATTTTGCAAATAAATTTCAATTTCAAATTCAGCATTATTATTAAAAAAAAAAAAAAATCCCAAATTTTACTGGTTTACAGTAGATGGACCAAAGCCCAGAGCATGATGGGTCAAAACCCACGATCAGGTAAAACGACGCCGCTTTTGCAAAATCCAGCATGATGTGAGCATATACGAAGAGTTCCGCTCCGTGAAAGCAGTGGCGCCGGCGCGTACGTTAGTCTAGGGTTTTAGCGAACGGTTAGATTTGGAAAGAATTAGGGTTTAAGGCAGAGCGAGAAGAGAAGAAGAAGAGTAGAAAGAACGATGCTGTACATTATAGGTTTAGGATTGGGCGACGAAAAGGACATCACTCTCAGAGGCTTAGAATGTGTGAAGAAATGCAACAAGATTTACATGGAAGCTTACACTTCTCTCTTGTCCTTTGGCCTTGCTTCCGATGGAATTTCTTCGCTCGAGAAGCTCTACGGAAAGACCATCACAGTTGCGGATCGGGAAATGGTCGAGGAGAAGGCTGATGAGATGCTCTCCAAGGCTCGTACCTCTGATGTTGCCTTTTTGGTTGTTGGCGACGCTTTCGGAGCTACGACCCATTCGGATCTGGTCGTTCGAGCAAAGAGTTTGGGGATTGAAGTCAGAGTGGTGTACAATGCTTCTGTGATGAATGCAATTGGAATTTGTGGGTTGCAACTGTACCGCTATGGTGAGACTGTTTCGATACCGTTTTTTACTGAAACGTGGAAACCGAGTAGCTTTTATGAGAAGATTCAAAGAAACCGTGGACTTGGATTGCACACACTCTGCTTATTAGATATACGAGTAAAGGAACCGTCTTTGGAGTCTTTATGCAGGGGAAGAAAAGAGTATGAACCACCTAGATTTATGTCTATAAACACAGCAATCGAGCAGCTTTTGGAGGTTGAGCAAATGGAAGGACAATCTGTATACAACGAAGACTCACTGTGTGTGGGATTTGCTCGTCTCGGAAGCGAAGATCAGATGATAGTAGCGGGCACCATGAAGCAGCTGAGAAGCATTGATTTCGGGCCGCCTCTGCATTGCCTTGTGATTGTAGGAAAGACCCATCCTGTTGAAGAAGAAATGTTGGACTTTTTTAAATCTGGAAATGAGAATCTTGAACAGGATGTCATTGACAGAACTACATAATTATTATCTGAGAGCCGAGATCCTTTCATTTACAGGTTATCCAAGTCCCGTTGTACCATTGCTTTTGAAAACTTTGAAATCTGCTTTACTTATATTTTAAAGTTATTTATATATATTATAGAACTCTTGGATTTCTTGGTTTTGACTGCTCAAAAATCAGTTATCTTAAACATTACAGCTGATTAGAGATAAATTTCAGAAATATTTCTGCAAATCATTGAATGGTTTATGGAAC

Coding sequence (CDS)

ATGCTGTACATTATAGGTTTAGGATTGGGCGACGAAAAGGACATCACTCTCAGAGGCTTAGAATGTGTGAAGAAATGCAACAAGATTTACATGGAAGCTTACACTTCTCTCTTGTCCTTTGGCCTTGCTTCCGATGGAATTTCTTCGCTCGAGAAGCTCTACGGAAAGACCATCACAGTTGCGGATCGGGAAATGGTCGAGGAGAAGGCTGATGAGATGCTCTCCAAGGCTCGTACCTCTGATGTTGCCTTTTTGGTTGTTGGCGACGCTTTCGGAGCTACGACCCATTCGGATCTGGTCGTTCGAGCAAAGAGTTTGGGGATTGAAGTCAGAGTGGTGTACAATGCTTCTGTGATGAATGCAATTGGAATTTGTGGGTTGCAACTGTACCGCTATGGTGAGACTGTTTCGATACCGTTTTTTACTGAAACGTGGAAACCGAGTAGCTTTTATGAGAAGATTCAAAGAAACCGTGGACTTGGATTGCACACACTCTGCTTATTAGATATACGAGTAAAGGAACCGTCTTTGGAGTCTTTATGCAGGGGAAGAAAAGAGTATGAACCACCTAGATTTATGTCTATAAACACAGCAATCGAGCAGCTTTTGGAGGTTGAGCAAATGGAAGGACAATCTGTATACAACGAAGACTCACTGTGTGTGGGATTTGCTCGTCTCGGAAGCGAAGATCAGATGATAGTAGCGGGCACCATGAAGCAGCTGAGAAGCATTGATTTCGGGCCGCCTCTGCATTGCCTTGTGATTGTAGGAAAGACCCATCCTGTTGAAGAAGAAATGTTGGACTTTTTTAAATCTGGAAATGAGAATCTTGAACAGGATGTCATTGACAGAACTACATAA

Protein sequence

MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITVADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMNAIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESLCRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQLRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT
Homology
BLAST of MC08g0735 vs. ExPASy Swiss-Prot
Match: O81769 (Probable diphthine methyl ester synthase OS=Arabidopsis thaliana OX=3702 GN=At4g31790 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 8.7e-127
Identity = 222/277 (80.14%), Postives = 250/277 (90.25%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLE VKK  K+YMEAYTSLLSFGL++DG+S+LEK YGK I +
Sbjct: 1   MLYIIGLGLGDEKDITLRGLEAVKKSQKVYMEAYTSLLSFGLSADGLSNLEKFYGKPIIL 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKA +M+ +A  +DVAFLVVGD FGATTHSDLVVRAK+LG++V VV+NASVMN
Sbjct: 61  ADREMVEEKAGDMIDEAIDNDVAFLVVGDPFGATTHSDLVVRAKTLGVKVEVVHNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           A+GICGLQLY YGETVSIPFFTETW+P SFYEKI++NR LGLHTLCLLDIRVKEP+ ESL
Sbjct: 121 AVGICGLQLYHYGETVSIPFFTETWRPDSFYEKIKKNRSLGLHTLCLLDIRVKEPTFESL 180

Query: 181 CR-GRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMK 240
           CR G+K+YEPPR+MS+NTAIEQLLEVEQ  G SVY ED+ CVGFARLGSEDQ IVAGTMK
Sbjct: 181 CRGGKKQYEPPRYMSVNTAIEQLLEVEQKHGDSVYGEDTQCVGFARLGSEDQTIVAGTMK 240

Query: 241 QLRSIDFGPPLHCLVIVGKTHPVEEEMLDF--FKSGN 275
           QL S+DFG PLHCLVIVG+THPVEEEML+F  +KSGN
Sbjct: 241 QLESVDFGAPLHCLVIVGETHPVEEEMLEFYKYKSGN 277

BLAST of MC08g0735 vs. ExPASy Swiss-Prot
Match: Q75JG8 (Diphthine methyl ester synthase OS=Dictyostelium discoideum OX=44689 GN=dph5 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-94
Identity = 160/271 (59.04%), Postives = 215/271 (79.34%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           +LYIIGLGLGDEKD+T++G E +KK +KIY+EAYTSLL     S  I +LEK Y K I +
Sbjct: 2   VLYIIGLGLGDEKDVTIKGFEAIKKSSKIYLEAYTSLLG---GSTSIEALEKFYEKKIII 61

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVE   +EML ++  +DV+FLVVGD FGATTH+DLV+RAK L I V+V++NAS+MN
Sbjct: 62  ADREMVESGCEEMLKESTENDVSFLVVGDPFGATTHTDLVIRAKELSIPVKVIHNASIMN 121

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIG CGLQLY YG+T+S+ FFTET KP S+Y++++ NR  G+HTLCLLDI+VKE S+E++
Sbjct: 122 AIGCCGLQLYSYGQTISMVFFTETTKPDSWYDRVKINRVNGMHTLCLLDIKVKEQSIENM 181

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRGR  YEPPRFM++N  IEQLLE+E++  + VY++D+LC+G +R+G +DQ I++GTMK+
Sbjct: 182 CRGRLIYEPPRFMTVNQCIEQLLEIEEIRKEKVYDQDTLCIGLSRVGQDDQQIISGTMKE 241

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFK 272
           L  +DFG PLH  +I G  H +E+E  + F+
Sbjct: 242 LLDVDFGAPLHSFIICGDMHFIEKEYFETFR 269

BLAST of MC08g0735 vs. ExPASy Swiss-Prot
Match: Q9H2P9 (Diphthine methyl ester synthase OS=Homo sapiens OX=9606 GN=DPH5 PE=1 SV=2)

HSP 1 Score: 337.4 bits (864), Expect = 1.6e-91
Identity = 163/288 (56.60%), Postives = 220/288 (76.39%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLY+IGLGLGD KDIT++GLE V++C+++Y+EAYTS+L+ G       +LE+ YG+ + V
Sbjct: 1   MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGK-----EALEEFYGRKLVV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADRE VE++AD +L  A  SDVAFLVVGD FGATTHSDLV+RA  LGI  RV++NAS+MN
Sbjct: 61  ADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           A+G CGLQLY++GETVSI F+T+TW+P SF++K+++NR  G+HTLCLLDI+VKE SLE+L
Sbjct: 121 AVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQME----GQSVYNEDSLCVGFARLGSEDQMIVAG 240
            +GRK YEPPR+MS+N A +QLLE+ Q +     +    E++LCVG AR+G++DQ I AG
Sbjct: 181 IKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAG 240

Query: 241 TMKQLRSIDFGPPLHCLVIV-GKTHPVEEEMLDFFKSGNENLEQDVID 284
           T++Q+ ++D G PLH L+I  G  HP+E EML  F     + E   I+
Sbjct: 241 TLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSIN 283

BLAST of MC08g0735 vs. ExPASy Swiss-Prot
Match: Q9CWQ0 (Diphthine methyl ester synthase OS=Mus musculus OX=10090 GN=Dph5 PE=1 SV=2)

HSP 1 Score: 334.7 bits (857), Expect = 1.0e-90
Identity = 161/275 (58.55%), Postives = 213/275 (77.45%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLY+IGLGLGD KDIT++GLE V++C+++Y+EAYTS+L+ G       +LE+ YG+ + +
Sbjct: 1   MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGK-----EALEEFYGRKLIL 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADRE VE++AD +   A  SDVAFLVVGD FGATTHSDL++RA  LGI  +V++NAS+MN
Sbjct: 61  ADREEVEQEADNIFKDADVSDVAFLVVGDPFGATTHSDLILRATKLGIPYQVIHNASIMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           A+G CGLQLYR+GETVSI F+T+TW+P SF++K++RNR  G+HTLCLLDI+VKE SLE+L
Sbjct: 121 AVGCCGLQLYRFGETVSIVFWTDTWRPESFFDKVKRNRANGMHTLCLLDIKVKEQSLENL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQME----GQSVYNEDSLCVGFARLGSEDQMIVAG 240
            RGRK YEPPR+MS+N A +QLLE+ Q       +    E++LCVG AR+G+EDQ I AG
Sbjct: 181 IRGRKIYEPPRYMSVNQAAQQLLEIVQNHRARGEEPAITEETLCVGLARVGAEDQKIAAG 240

Query: 241 TMKQLRSIDFGPPLHCLVIV-GKTHPVEEEMLDFF 271
           T++Q+ ++  G PLH LVI  G  HP+E EML  F
Sbjct: 241 TLQQMCTVSLGEPLHSLVITGGNLHPLEMEMLSLF 270

BLAST of MC08g0735 vs. ExPASy Swiss-Prot
Match: Q5E982 (Diphthine methyl ester synthase OS=Bos taurus OX=9913 GN=DPH5 PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.5e-89
Identity = 158/275 (57.45%), Postives = 213/275 (77.45%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLY+IGLGLGD KDIT++GLE V++C+++Y+E YTS+L+ G        LE+ Y + + +
Sbjct: 1   MLYMIGLGLGDAKDITVKGLEVVRRCSRVYLETYTSVLTVGK-----EVLEEFYERKLIL 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADRE VE++AD +L  A  SDVAFLVVGD FGATTHSDL++RA  LGI  RV++NAS+MN
Sbjct: 61  ADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLILRATKLGIPYRVIHNASIMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           A+G CGLQLY++GETVSI F+T+TW+P SF++K+++NR  G+HTLCLLDI+VKE SLE+L
Sbjct: 121 AVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQME----GQSVYNEDSLCVGFARLGSEDQMIVAG 240
            +GRK YEPPR+MS+N A +QLLE+ Q +     +    E++LCVG AR+G+EDQ I AG
Sbjct: 181 IKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGAEDQKIAAG 240

Query: 241 TMKQLRSIDFGPPLHCLVIV-GKTHPVEEEMLDFF 271
           T++Q+ ++D G PLH L+I  G  HP+E EML  F
Sbjct: 241 TLQQMSTVDLGGPLHSLIITGGSLHPLEMEMLSLF 270

BLAST of MC08g0735 vs. NCBI nr
Match: XP_022144138.1 (probable diphthine methyl ester synthase [Momordica charantia])

HSP 1 Score: 570 bits (1468), Expect = 8.07e-205
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ
Sbjct: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286
           LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT
Sbjct: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286

BLAST of MC08g0735 vs. NCBI nr
Match: XP_008444788.1 (PREDICTED: probable diphthine methyl ester synthase [Cucumis melo] >KAA0065172.1 putative diphthine methyl ester synthase [Cucumis melo var. makuwa])

HSP 1 Score: 551 bits (1419), Expect = 2.38e-197
Identity = 272/286 (95.10%), Postives = 283/286 (98.95%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLECV+KC K+YMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLYIIGLGLGDEKDITLRGLECVRKCEKVYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKAD++LS+ARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADQILSEARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQ+NRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQKNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRG+KEYEPPRFMSINTAIEQLLEVEQMEGQSVYNED+LCV FARLGSEDQMI AGTMKQ
Sbjct: 181 CRGKKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDTLCVAFARLGSEDQMITAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286
           LRSIDFGPPLHCLVIVGKTHPVEEEMLDF+KSGNENLEQ+V+DRTT
Sbjct: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFYKSGNENLEQNVVDRTT 286

BLAST of MC08g0735 vs. NCBI nr
Match: XP_004152655.1 (probable diphthine methyl ester synthase [Cucumis sativus] >KGN62668.1 hypothetical protein Csa_022621 [Cucumis sativus])

HSP 1 Score: 550 bits (1416), Expect = 6.83e-197
Identity = 271/286 (94.76%), Postives = 283/286 (98.95%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLECV+KC K+YMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLYIIGLGLGDEKDITLRGLECVRKCEKVYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKAD++LS+ARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADQILSEARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           A+GICGLQLYRYGETVSIPFFTETWKPSSFYEKIQ+NRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AVGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQKNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRG+KEYEPPRFMSINTAIEQLLEVEQMEGQSVYNED+LCV FARLGSEDQ IVAGTMKQ
Sbjct: 181 CRGKKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDTLCVAFARLGSEDQTIVAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286
           LRSIDFGPPLHCLVIVGKTHPVEEEMLDF+KSGNENLEQ+V+DRTT
Sbjct: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFYKSGNENLEQNVVDRTT 286

BLAST of MC08g0735 vs. NCBI nr
Match: XP_023547198.1 (probable diphthine methyl ester synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540 bits (1390), Expect = 6.27e-193
Identity = 267/282 (94.68%), Postives = 278/282 (98.58%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           ML+IIGLGLGDEKDITLRGLECV+KC K+YMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLFIIGLGLGDEKDITLRGLECVRKCEKVYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKAD++LS+ARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADQILSEARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQ+NRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQKNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYN+D+LCVGFARLGSE QMIVAGTMKQ
Sbjct: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNDDTLCVGFARLGSESQMIVAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVI 282
           LRSIDFG PLHCLVIVGKTHPVEEEMLDF+KSG ENLEQDV+
Sbjct: 241 LRSIDFGSPLHCLVIVGKTHPVEEEMLDFYKSGTENLEQDVV 282

BLAST of MC08g0735 vs. NCBI nr
Match: XP_022961663.1 (probable diphthine methyl ester synthase [Cucurbita moschata] >XP_022996987.1 probable diphthine methyl ester synthase [Cucurbita maxima] >KAG6598494.1 putative diphthine methyl ester synthase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029430.1 putative diphthine methyl ester synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540 bits (1390), Expect = 6.27e-193
Identity = 267/282 (94.68%), Postives = 278/282 (98.58%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           ML+IIGLGLGDEKDITLRGLECV+KC K+YMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLFIIGLGLGDEKDITLRGLECVRKCEKVYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKAD++LS+ARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADQILSEARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQ+NRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQKNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYN+D+LCVGFARLGSE QMIVAGTMKQ
Sbjct: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNDDTLCVGFARLGSESQMIVAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVI 282
           LRSIDFG PLHCLVIVGKTHPVEEEMLDF+KSG ENLEQDV+
Sbjct: 241 LRSIDFGSPLHCLVIVGKTHPVEEEMLDFYKSGTENLEQDVV 282

BLAST of MC08g0735 vs. ExPASy TrEMBL
Match: A0A6J1CSI4 (Diphthine methyl ester synthase OS=Momordica charantia OX=3673 GN=LOC111013896 PE=3 SV=1)

HSP 1 Score: 570 bits (1468), Expect = 3.91e-205
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ
Sbjct: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286
           LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT
Sbjct: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286

BLAST of MC08g0735 vs. ExPASy TrEMBL
Match: A0A5A7VA24 (Diphthine methyl ester synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005090 PE=3 SV=1)

HSP 1 Score: 551 bits (1419), Expect = 1.15e-197
Identity = 272/286 (95.10%), Postives = 283/286 (98.95%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLECV+KC K+YMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLYIIGLGLGDEKDITLRGLECVRKCEKVYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKAD++LS+ARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADQILSEARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQ+NRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQKNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRG+KEYEPPRFMSINTAIEQLLEVEQMEGQSVYNED+LCV FARLGSEDQMI AGTMKQ
Sbjct: 181 CRGKKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDTLCVAFARLGSEDQMITAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286
           LRSIDFGPPLHCLVIVGKTHPVEEEMLDF+KSGNENLEQ+V+DRTT
Sbjct: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFYKSGNENLEQNVVDRTT 286

BLAST of MC08g0735 vs. ExPASy TrEMBL
Match: A0A1S3BC13 (Diphthine methyl ester synthase OS=Cucumis melo OX=3656 GN=LOC103488034 PE=3 SV=1)

HSP 1 Score: 551 bits (1419), Expect = 1.15e-197
Identity = 272/286 (95.10%), Postives = 283/286 (98.95%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLECV+KC K+YMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLYIIGLGLGDEKDITLRGLECVRKCEKVYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKAD++LS+ARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADQILSEARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQ+NRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQKNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRG+KEYEPPRFMSINTAIEQLLEVEQMEGQSVYNED+LCV FARLGSEDQMI AGTMKQ
Sbjct: 181 CRGKKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDTLCVAFARLGSEDQMITAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286
           LRSIDFGPPLHCLVIVGKTHPVEEEMLDF+KSGNENLEQ+V+DRTT
Sbjct: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFYKSGNENLEQNVVDRTT 286

BLAST of MC08g0735 vs. ExPASy TrEMBL
Match: A0A0A0LLL7 (Diphthine methyl ester synthase OS=Cucumis sativus OX=3659 GN=Csa_2G367220 PE=3 SV=1)

HSP 1 Score: 550 bits (1416), Expect = 3.31e-197
Identity = 271/286 (94.76%), Postives = 283/286 (98.95%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLECV+KC K+YMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLYIIGLGLGDEKDITLRGLECVRKCEKVYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKAD++LS+ARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADQILSEARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           A+GICGLQLYRYGETVSIPFFTETWKPSSFYEKIQ+NRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AVGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQKNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRG+KEYEPPRFMSINTAIEQLLEVEQMEGQSVYNED+LCV FARLGSEDQ IVAGTMKQ
Sbjct: 181 CRGKKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDTLCVAFARLGSEDQTIVAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVIDRTT 286
           LRSIDFGPPLHCLVIVGKTHPVEEEMLDF+KSGNENLEQ+V+DRTT
Sbjct: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFYKSGNENLEQNVVDRTT 286

BLAST of MC08g0735 vs. ExPASy TrEMBL
Match: A0A6J1K3K2 (Diphthine methyl ester synthase OS=Cucurbita maxima OX=3661 GN=LOC111492052 PE=3 SV=1)

HSP 1 Score: 540 bits (1390), Expect = 3.04e-193
Identity = 267/282 (94.68%), Postives = 278/282 (98.58%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           ML+IIGLGLGDEKDITLRGLECV+KC K+YMEAYTSLLSFGLASDGISSLEKLYGKTITV
Sbjct: 1   MLFIIGLGLGDEKDITLRGLECVRKCEKVYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKAD++LS+ARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN
Sbjct: 61  ADREMVEEKADQILSEARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQ+NRGLGLHTLCLLDIRVKEPSLESL
Sbjct: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQKNRGLGLHTLCLLDIRVKEPSLESL 180

Query: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMKQ 240
           CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYN+D+LCVGFARLGSE QMIVAGTMKQ
Sbjct: 181 CRGRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNDDTLCVGFARLGSESQMIVAGTMKQ 240

Query: 241 LRSIDFGPPLHCLVIVGKTHPVEEEMLDFFKSGNENLEQDVI 282
           LRSIDFG PLHCLVIVGKTHPVEEEMLDF+KSG ENLEQDV+
Sbjct: 241 LRSIDFGSPLHCLVIVGKTHPVEEEMLDFYKSGTENLEQDVV 282

BLAST of MC08g0735 vs. TAIR 10
Match: AT4G31790.1 (Tetrapyrrole (Corrin/Porphyrin) Methylases )

HSP 1 Score: 454.5 bits (1168), Expect = 6.2e-128
Identity = 222/277 (80.14%), Postives = 250/277 (90.25%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLE VKK  K+YMEAYTSLLSFGL++DG+S+LEK YGK I +
Sbjct: 1   MLYIIGLGLGDEKDITLRGLEAVKKSQKVYMEAYTSLLSFGLSADGLSNLEKFYGKPIIL 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKA +M+ +A  +DVAFLVVGD FGATTHSDLVVRAK+LG++V VV+NASVMN
Sbjct: 61  ADREMVEEKAGDMIDEAIDNDVAFLVVGDPFGATTHSDLVVRAKTLGVKVEVVHNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           A+GICGLQLY YGETVSIPFFTETW+P SFYEKI++NR LGLHTLCLLDIRVKEP+ ESL
Sbjct: 121 AVGICGLQLYHYGETVSIPFFTETWRPDSFYEKIKKNRSLGLHTLCLLDIRVKEPTFESL 180

Query: 181 CR-GRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMK 240
           CR G+K+YEPPR+MS+NTAIEQLLEVEQ  G SVY ED+ CVGFARLGSEDQ IVAGTMK
Sbjct: 181 CRGGKKQYEPPRYMSVNTAIEQLLEVEQKHGDSVYGEDTQCVGFARLGSEDQTIVAGTMK 240

Query: 241 QLRSIDFGPPLHCLVIVGKTHPVEEEMLDF--FKSGN 275
           QL S+DFG PLHCLVIVG+THPVEEEML+F  +KSGN
Sbjct: 241 QLESVDFGAPLHCLVIVGETHPVEEEMLEFYKYKSGN 277

BLAST of MC08g0735 vs. TAIR 10
Match: AT4G31790.2 (Tetrapyrrole (Corrin/Porphyrin) Methylases )

HSP 1 Score: 454.5 bits (1168), Expect = 6.2e-128
Identity = 222/277 (80.14%), Postives = 250/277 (90.25%), Query Frame = 0

Query: 1   MLYIIGLGLGDEKDITLRGLECVKKCNKIYMEAYTSLLSFGLASDGISSLEKLYGKTITV 60
           MLYIIGLGLGDEKDITLRGLE VKK  K+YMEAYTSLLSFGL++DG+S+LEK YGK I +
Sbjct: 1   MLYIIGLGLGDEKDITLRGLEAVKKSQKVYMEAYTSLLSFGLSADGLSNLEKFYGKPIIL 60

Query: 61  ADREMVEEKADEMLSKARTSDVAFLVVGDAFGATTHSDLVVRAKSLGIEVRVVYNASVMN 120
           ADREMVEEKA +M+ +A  +DVAFLVVGD FGATTHSDLVVRAK+LG++V VV+NASVMN
Sbjct: 61  ADREMVEEKAGDMIDEAIDNDVAFLVVGDPFGATTHSDLVVRAKTLGVKVEVVHNASVMN 120

Query: 121 AIGICGLQLYRYGETVSIPFFTETWKPSSFYEKIQRNRGLGLHTLCLLDIRVKEPSLESL 180
           A+GICGLQLY YGETVSIPFFTETW+P SFYEKI++NR LGLHTLCLLDIRVKEP+ ESL
Sbjct: 121 AVGICGLQLYHYGETVSIPFFTETWRPDSFYEKIKKNRSLGLHTLCLLDIRVKEPTFESL 180

Query: 181 CR-GRKEYEPPRFMSINTAIEQLLEVEQMEGQSVYNEDSLCVGFARLGSEDQMIVAGTMK 240
           CR G+K+YEPPR+MS+NTAIEQLLEVEQ  G SVY ED+ CVGFARLGSEDQ IVAGTMK
Sbjct: 181 CRGGKKQYEPPRYMSVNTAIEQLLEVEQKHGDSVYGEDTQCVGFARLGSEDQTIVAGTMK 240

Query: 241 QLRSIDFGPPLHCLVIVGKTHPVEEEMLDF--FKSGN 275
           QL S+DFG PLHCLVIVG+THPVEEEML+F  +KSGN
Sbjct: 241 QLESVDFGAPLHCLVIVGETHPVEEEMLEFYKYKSGN 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O817698.7e-12780.14Probable diphthine methyl ester synthase OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
Q75JG81.2e-9459.04Diphthine methyl ester synthase OS=Dictyostelium discoideum OX=44689 GN=dph5 PE=... [more]
Q9H2P91.6e-9156.60Diphthine methyl ester synthase OS=Homo sapiens OX=9606 GN=DPH5 PE=1 SV=2[more]
Q9CWQ01.0e-9058.55Diphthine methyl ester synthase OS=Mus musculus OX=10090 GN=Dph5 PE=1 SV=2[more]
Q5E9822.5e-8957.45Diphthine methyl ester synthase OS=Bos taurus OX=9913 GN=DPH5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022144138.18.07e-205100.00probable diphthine methyl ester synthase [Momordica charantia][more]
XP_008444788.12.38e-19795.10PREDICTED: probable diphthine methyl ester synthase [Cucumis melo] >KAA0065172.1... [more]
XP_004152655.16.83e-19794.76probable diphthine methyl ester synthase [Cucumis sativus] >KGN62668.1 hypotheti... [more]
XP_023547198.16.27e-19394.68probable diphthine methyl ester synthase [Cucurbita pepo subsp. pepo][more]
XP_022961663.16.27e-19394.68probable diphthine methyl ester synthase [Cucurbita moschata] >XP_022996987.1 pr... [more]
Match NameE-valueIdentityDescription
A0A6J1CSI43.91e-205100.00Diphthine methyl ester synthase OS=Momordica charantia OX=3673 GN=LOC111013896 P... [more]
A0A5A7VA241.15e-19795.10Diphthine methyl ester synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BC131.15e-19795.10Diphthine methyl ester synthase OS=Cucumis melo OX=3656 GN=LOC103488034 PE=3 SV=... [more]
A0A0A0LLL73.31e-19794.76Diphthine methyl ester synthase OS=Cucumis sativus OX=3659 GN=Csa_2G367220 PE=3 ... [more]
A0A6J1K3K23.04e-19394.68Diphthine methyl ester synthase OS=Cucurbita maxima OX=3661 GN=LOC111492052 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G31790.16.2e-12880.14Tetrapyrrole (Corrin/Porphyrin) Methylases [more]
AT4G31790.26.2e-12880.14Tetrapyrrole (Corrin/Porphyrin) Methylases [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004551Diphthine synthasePIRSFPIRSF036432Diphthine_synthcoord: 1..277
e-value: 3.0E-105
score: 349.4
IPR004551Diphthine synthaseTIGRFAMTIGR00522TIGR00522coord: 1..270
e-value: 6.6E-95
score: 315.3
IPR004551Diphthine synthasePANTHERPTHR10882DIPHTHINE SYNTHASEcoord: 1..271
IPR004551Diphthine synthaseHAMAPMF_01084Diphthine_synthcoord: 1..273
score: 27.573988
IPR004551Diphthine synthaseCDDcd11647DHP5_DphBcoord: 1..259
e-value: 9.88978E-136
score: 381.379
IPR014777Tetrapyrrole methylase, subdomain 1GENE3D3.40.1010.10coord: 1..116
e-value: 2.0E-41
score: 142.9
IPR000878Tetrapyrrole methylasePFAMPF00590TP_methylasecoord: 1..241
e-value: 6.4E-22
score: 78.5
IPR014776Tetrapyrrole methylase, subdomain 2GENE3D3.30.950.10coord: 117..274
e-value: 2.5E-75
score: 253.6
NoneNo IPR availablePIRSRPIRSR036432-1PIRSR036432-1coord: 1..171
e-value: 1.1E-51
score: 173.1
NoneNo IPR availablePANTHERPTHR10882:SF0DIPHTHINE METHYL ESTER SYNTHASEcoord: 1..271
IPR035996Tetrapyrrole methylase superfamilySUPERFAMILY53790Tetrapyrrole methylasecoord: 1..277

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0735.1MC08g0735.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine
molecular_function GO:0004164 diphthine synthase activity
molecular_function GO:0008168 methyltransferase activity