MC08g0714 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g0714 vs. ExPASy Swiss-Prot
Match: Q9SJT7 (V-type proton ATPase subunit a2 OS=Arabidopsis thaliana OX=3702 GN=VHA-a2 PE=1 SV=1)

HSP 1 Score: 1221.1 bits (3158), Expect = 0.0e+00
Identity = 595/809 (73.55%), Postives = 704/809 (87.02%), Query Frame = 0

Query: 1   PMDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGE 60
           PMDL+RSEPMQL Q+I+P ESAH T+SYLGDLGL QF DLN+ +SPFQRTYA  IKRCGE
Sbjct: 14  PMDLMRSEPMQLVQVIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAAQIKRCGE 73

Query: 61  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 120
           MARK+RFF++QM++AG++P         +DLD++EVKL ELEAEL+EIN NN+KLQR+++
Sbjct: 74  MARKIRFFKEQMSKAGVTPKETLDRENDIDLDDVEVKLEELEAELVEINANNDKLQRSYN 133

Query: 121 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 180
           EL+EYKLVL+K GEFF  A   A AQQ E+E +Q  E  +++P LL++E + DP KQVKL
Sbjct: 134 ELVEYKLVLEKAGEFFASAHRSATAQQSEIETEQVGEDLLEAP-LLQEEKSVDPTKQVKL 193

Query: 181 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 240
           G+++GLVPREKSM FERILFR+TRGN+++RQ+V++ SV DP SGEK EKNVFV+F+SGER
Sbjct: 194 GFLTGLVPREKSMVFERILFRATRGNIFIRQSVIEESVVDPNSGEKAEKNVFVVFYSGER 253

Query: 241 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 300
           AK+KILKICEAFGANRYPF++DLGKQ QM+TEVSG+LSELKTTI  G   R+ L +TIG 
Sbjct: 254 AKSKILKICEAFGANRYPFSEDLGKQAQMMTEVSGRLSELKTTIGAGLDQRNILLETIGD 313

Query: 301 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQI 360
           ++EQWNL ++KEK+IYHTLNMLS+DVTKKCLVGEGW PV A  +IQ+A+H+A  DS SQ+
Sbjct: 314 KFEQWNLKIRKEKAIYHTLNMLSLDVTKKCLVGEGWSPVFAATEIQDALHRAAVDSNSQV 373

Query: 361 EAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFG 420
            +IF VLRTKE PPT+FRTNKFT++FQEIVDAYGVAKYQEANP V+TI+TFPFLFAVMFG
Sbjct: 374 GSIFQVLRTKEMPPTFFRTNKFTTAFQEIVDAYGVAKYQEANPSVFTIVTFPFLFAVMFG 433

Query: 421 DWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFS 480
           DWGHGICLLLAT+Y I REKK SS+KLGDI+EM FGGRYVI MM+LFSIYTGLIYNEFFS
Sbjct: 434 DWGHGICLLLATMYLILREKKLSSQKLGDIMEMAFGGRYVIFMMSLFSIYTGLIYNEFFS 493

Query: 481 VPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSI 540
           +P+ LF  SAY CRD SC +ATTIGLIK RDTY FGVDP WHGTRSELPFLNSLKMKMSI
Sbjct: 494 IPYPLFASSAYDCRDVSCSEATTIGLIKTRDTYPFGVDPVWHGTRSELPFLNSLKMKMSI 553

Query: 541 LLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQAD 600
           L+GVAQMNLGII+S+FNAKFF   +NIW+QFVPQMIFLN LFGYLS+LII+KWC+GSQAD
Sbjct: 554 LIGVAQMNLGIIMSFFNAKFFKSAVNIWFQFVPQMIFLNCLFGYLSVLIIIKWCTGSQAD 613

Query: 601 LYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQ 660
           LYH+MIYMFLSP DDLGENQLFP QK +QL  L  AL++VPWMLLPKPF+LKKQHE RHQ
Sbjct: 614 LYHVMIYMFLSPMDDLGENQLFPNQKIVQLTFLFLALVSVPWMLLPKPFILKKQHEARHQ 673

Query: 661 GQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWA 720
           G SY+ +  TD++ + E + G H HEEF+FSE+FVHQLIHTIEFVLGAVSNTASYLRLWA
Sbjct: 674 GLSYAQLDETDESLQVETNGGGHGHEEFEFSEIFVHQLIHTIEFVLGAVSNTASYLRLWA 733

Query: 721 LSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLH 780
           LSLAHSELSSVFY+K+LL+AWGFN++ I  VG++VFI ATVGVLLVMETLSAFLHALRLH
Sbjct: 734 LSLAHSELSSVFYEKVLLMAWGFNNVFIWIVGILVFIFATVGVLLVMETLSAFLHALRLH 793

Query: 781 WVEFQNKFYAGDGFKFSPFSFSSLRDEDE 810
           WVE+QNKFY GDG+KF+PF+F+ + +EDE
Sbjct: 794 WVEYQNKFYEGDGYKFAPFTFTLVGNEDE 821

BLAST of MC08g0714 vs. ExPASy Swiss-Prot
Match: Q8W4S4 (V-type proton ATPase subunit a3 OS=Arabidopsis thaliana OX=3702 GN=VHA-a3 PE=1 SV=1)

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 602/810 (74.32%), Postives = 704/810 (86.91%), Query Frame = 0

Query: 1   PMDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGE 60
           PMDL+RSE MQL QLI+P ESAH T+SYLGDLGL QF DLN+ +SPFQRTYA  IKRCGE
Sbjct: 13  PMDLMRSETMQLVQLIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAAQIKRCGE 72

Query: 61  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 120
           MARK+RFFRDQM++AG+           +DLD++EVKLGELEAEL+EIN NN+KLQR+++
Sbjct: 73  MARKIRFFRDQMSKAGVPAKEMQGKENDIDLDDVEVKLGELEAELVEINANNDKLQRSYN 132

Query: 121 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 180
           EL+EYKLVLQK GEFF  A   AA QQRE E QQ  E  ++SP LL++E + D  KQVKL
Sbjct: 133 ELMEYKLVLQKAGEFFSSAHRSAADQQRETESQQAGEDLLESP-LLQEEKSIDSTKQVKL 192

Query: 181 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 240
           G+++GLVPREKSM FERILFR+TRGN+++RQ V++  V DP SGEK EKNVFV+F+SGER
Sbjct: 193 GFLTGLVPREKSMVFERILFRATRGNIFIRQTVIEEPVIDPNSGEKAEKNVFVVFYSGER 252

Query: 241 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 300
           AK+KILKICEAFGANRYPF++DLG+Q QMITEVSG+LSELKTTID G   R+ L QTIG 
Sbjct: 253 AKSKILKICEAFGANRYPFSEDLGRQAQMITEVSGRLSELKTTIDAGLGQRNILLQTIGD 312

Query: 301 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQI 360
           ++E WNL V+KEK+IYHTLNMLS+DVTKKCLV EGW PV A+ +IQ+A+ +A  DS SQ+
Sbjct: 313 KFELWNLKVRKEKAIYHTLNMLSLDVTKKCLVAEGWSPVFASREIQDALQRAAVDSNSQV 372

Query: 361 EAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFG 420
            +IF VLRTKE+PPTYFRTNKFTS+ QEIVDAYGVAKYQEANPGV+TI+TFPFLFAVMFG
Sbjct: 373 GSIFQVLRTKESPPTYFRTNKFTSAIQEIVDAYGVAKYQEANPGVFTIVTFPFLFAVMFG 432

Query: 421 DWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFS 480
           DWGHGIC+LLAT+Y I +EKK +S+KLGDI+EM FGGRYVI+MM+LFSIYTGLIYNEFFS
Sbjct: 433 DWGHGICILLATMYLILKEKKLASQKLGDIMEMAFGGRYVILMMSLFSIYTGLIYNEFFS 492

Query: 481 VPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSI 540
           +PF LF PSAY CRD SC +ATTIGLIKVRDTY FG+DP WHG+RSELPFLNSLKMKMSI
Sbjct: 493 IPFPLFAPSAYDCRDVSCSEATTIGLIKVRDTYPFGLDPVWHGSRSELPFLNSLKMKMSI 552

Query: 541 LLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQAD 600
           LLGV+QMNLGII+SYFNA+FF  ++NIW+QF+PQMIFLNSLFGYLS+LII+KWC+GSQAD
Sbjct: 553 LLGVSQMNLGIIMSYFNARFFKSSVNIWFQFIPQMIFLNSLFGYLSVLIIIKWCTGSQAD 612

Query: 601 LYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQ 660
           LYH+MIYMFLSP D+LGENQLFP QK LQL+LL  AL++VP MLLPKPF+LKKQHE RHQ
Sbjct: 613 LYHVMIYMFLSPMDELGENQLFPHQKTLQLVLLFLALVSVPCMLLPKPFILKKQHEARHQ 672

Query: 661 GQSYSLIHSTDDNDETE-HSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720
           GQ+Y+ +  TD++   E +  GSH HEEF+FSE+FVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 673 GQAYAPLDETDESLHVETNGGGSHGHEEFEFSEIFVHQLIHTIEFVLGAVSNTASYLRLW 732

Query: 721 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 780
           ALSLAHSELSSVFY+K+LLLAWG+N+ +I  VGV+VFI ATVGVLLVMETLSAFLHALRL
Sbjct: 733 ALSLAHSELSSVFYEKVLLLAWGYNNPLILIVGVLVFIFATVGVLLVMETLSAFLHALRL 792

Query: 781 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 810
           HWVEFQNKFY GDG+KF+PF+F    +EDE
Sbjct: 793 HWVEFQNKFYEGDGYKFAPFTFIFTANEDE 821

BLAST of MC08g0714 vs. ExPASy Swiss-Prot
Match: Q8RWZ7 (V-type proton ATPase subunit a1 OS=Arabidopsis thaliana OX=3702 GN=VHA-a1 PE=2 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 1.0e-297
Identity = 503/805 (62.48%), Postives = 634/805 (78.76%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDL+RSE M L QLIIP ESAHR+I+YLG+LGL QF DLNA +SPFQRT+A  +KRCGEM
Sbjct: 11  MDLMRSEKMTLVQLIIPVESAHRSITYLGELGLLQFRDLNADKSPFQRTFANQVKRCGEM 70

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           +RKLRFF+DQ+ +AGL  S      P + L +LE +L + E E+LE+N N+EKL++ ++E
Sbjct: 71  SRKLRFFKDQIDKAGLRCSPRLEIEPDIALGDLERQLADHEHEVLEMNSNSEKLRQTYNE 130

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQREL-EVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           LLE+K+VL+K   F   + + A  ++ EL E   +  G I++  LLEQEM      Q  L
Sbjct: 131 LLEFKIVLEKASGFLVSSNTHAIGEEIELHESTYSNNGFIETASLLEQEMNPGHSNQSGL 190

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
            +ISG++ ++K + FER+LFR+TRGNM   Q   D  + DP + E VEK VFV+FFSGE+
Sbjct: 191 RFISGIINKDKLLKFERMLFRATRGNMLFNQTTSDEEIMDPSTSEMVEKVVFVVFFSGEQ 250

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           A+ KILKICEAFGAN YP  +D  KQ Q+  EV  +LS+L+ T+D G  HR+    ++G+
Sbjct: 251 ARTKILKICEAFGANCYPVPEDTTKQRQLTREVLSRLSDLEATLDAGTRHRNNALNSVGY 310

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQI 361
               W   V++EK++Y TLNML+ DVTKKCLVGEGWCP  A  QI   + +AT+DS SQ+
Sbjct: 311 SLTNWITTVRREKAVYDTLNMLNFDVTKKCLVGEGWCPTFAKTQIHEVLQRATFDSSSQV 370

Query: 362 EAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFG 421
             IFHV++  E+PPTYFRTNK T++FQEI+DAYGVA+YQEANP VY+++T+PFLFAVMFG
Sbjct: 371 GVIFHVMQAVESPPTYFRTNKLTNAFQEIIDAYGVARYQEANPAVYSVVTYPFLFAVMFG 430

Query: 422 DWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFS 481
           DWGHG+CLLL  LY + RE+K S++KLG  +EM FGGRYVI++MALFSIY GLIYNEFFS
Sbjct: 431 DWGHGLCLLLGALYLLARERKLSTQKLGSFMEMLFGGRYVILLMALFSIYCGLIYNEFFS 490

Query: 482 VPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSI 541
           VPF +FG SAY CRDT+C DA T+GLIK RD Y FGVDP W G+R+ELP+LNSLKMKMSI
Sbjct: 491 VPFHIFGGSAYKCRDTTCSDAYTVGLIKYRDPYPFGVDPSWRGSRTELPYLNSLKMKMSI 550

Query: 542 LLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQAD 601
           LLG+AQMNLG+ILS+FNA+FFG +++I YQF+PQMIFLNSLFGYLSLLII+KWC+GSQAD
Sbjct: 551 LLGIAQMNLGLILSFFNARFFGSSLDIRYQFIPQMIFLNSLFGYLSLLIIIKWCTGSQAD 610

Query: 602 LYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQ 661
           LYH+MIYMFLSPT++LGEN+LF GQ+ LQ++LLL A IAVPWML PKPF L+K H ER Q
Sbjct: 611 LYHVMIYMFLSPTEELGENELFWGQRPLQIVLLLLAFIAVPWMLFPKPFALRKIHMERFQ 670

Query: 662 GQSYSLIHSTD---DNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLR 721
           G++Y ++ S++   D +      G H  EEF+FSE+FVHQLIH+IEFVLG+VSNTASYLR
Sbjct: 671 GRTYGVLVSSEVDLDVEPDSARGGGHHEEEFNFSEIFVHQLIHSIEFVLGSVSNTASYLR 730

Query: 722 LWALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHAL 781
           LWALSLAHSELS+VFY+K+LLLAWG+ +++IR +GV VF  AT  +LL+METLSAFLHAL
Sbjct: 731 LWALSLAHSELSTVFYEKVLLLAWGYENILIRLIGVAVFAFATAFILLMMETLSAFLHAL 790

Query: 782 RLHWVEFQNKFYAGDGFKFSPFSFS 803
           RLHWVEF  KF+ GDG+KF PFSF+
Sbjct: 791 RLHWVEFMGKFFNGDGYKFKPFSFA 815

BLAST of MC08g0714 vs. ExPASy Swiss-Prot
Match: Q54E04 (Vacuolar proton translocating ATPase 100 kDa subunit OS=Dictyostelium discoideum OX=44689 GN=vatM PE=1 SV=2)

HSP 1 Score: 649.8 bits (1675), Expect = 4.0e-185
Identity = 381/828 (46.01%), Postives = 528/828 (63.77%), Query Frame = 0

Query: 6   RSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEMARKL 65
           RS PMQ+ QL +  E+AH T+  LG LGL QF D N   + FQR +   +KRC +M +KL
Sbjct: 10  RSSPMQMVQLFVQIEAAHDTVDELGKLGLIQFLDDNEHVNLFQRNFVNEVKRCDDMEKKL 69

Query: 66  RFFRDQMAR----AGLSPSTNSLGVPGLD--LDNLEVKLGELEAELLEINDNNEKLQRNH 125
           +FF DQ+ +      L P  N L V   D  +D LE +  ELE+EL ++N N E LQRN+
Sbjct: 70  KFFEDQVKKEPKLQKLLPD-NMLSVVDDDSQMDELEGRFDELESELKQVNANQETLQRNY 129

Query: 126 SELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQ-V 185
           +EL++ + VL K   FFQ        +   L   +  E S  SPLL E +  ++  KQ V
Sbjct: 130 NELIQLRHVLTKDSVFFQ--------ENPNLIEGEGHEHSARSPLLAEDQHVSEVAKQGV 189

Query: 186 KLGYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSG 245
           KLG+I+G++  +K   F+R L+R+TRGN Y++ A ++  + DP +GE+  K VF++FF G
Sbjct: 190 KLGFITGVMNTDKMPQFQRSLWRTTRGNNYVKDARIEEEIIDPQTGEETAKTVFIVFFQG 249

Query: 246 ERAKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTI 305
           ER + KI KICE+FGAN Y   D+  ++  ++ +V+ ++++L   +   K H+ +    I
Sbjct: 250 ERLQQKIKKICESFGANIYDCPDNSFERSNLLQKVTVRITDLYEVLQRSKDHKRQTLAGI 309

Query: 306 GHQYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKS 365
             +   W   V  EKSIYHT+N+   DV +KCL+ +GW P     +IQ A+  AT  S +
Sbjct: 310 VPRLYSWKKKVLLEKSIYHTMNLFDYDVGRKCLIAKGWTPKDKIEEIQLALRTATTRSGA 369

Query: 366 QIEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVM 425
            + ++  +++T+ +PPT+F TNK+TSSFQEIV+AYG+A Y+E NP V TI+TFPFLF VM
Sbjct: 370 LVPSVLSIIKTEGSPPTHFETNKYTSSFQEIVNAYGIAHYREVNPAVLTIVTFPFLFGVM 429

Query: 426 FGDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEF 485
           FGD GHG  LLL+ L  I+ EKK + +KL ++I+M F GRYV+ +M+LFSIY G IYNE 
Sbjct: 430 FGDVGHGALLLLSALGLISLEKKLAGKKLNELIQMPFDGRYVLFLMSLFSIYVGFIYNEC 489

Query: 486 FSVPFELFGPSAYGCRDTSCRDATTIGLIKVRDT---YLFGVDPKWHGTRSELPFLNSLK 545
           FS+P  +FG S Y    T+       GL   + T   Y  GVDP W G  +EL + NS K
Sbjct: 490 FSIPMNIFG-SQYNLNSTT-------GLYTYQHTDRVYPVGVDPLWKGAPNELVYYNSFK 549

Query: 546 MKMSILLGVAQMNLGI---ILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVK 605
           MK+SI+ GV QM++GI   +L+Y N K   + +NI  QFVPQMIFL S+FGY+S+LII+K
Sbjct: 550 MKLSIIFGVVQMSVGICFSLLNYLNQKGPIKIVNILTQFVPQMIFLWSIFGYMSVLIILK 609

Query: 606 WC---SGSQAD------LYHIMIYMFLSP--TDDLGENQLFPGQKFLQLMLLLSALIAVP 665
           W       + D      +   +I MFLSP  T D+     F GQ  +Q  LL  ALI++P
Sbjct: 610 WVVPYRSFEVDKVDPPFILPTIIAMFLSPGGTPDV---VFFSGQGAVQTALLFLALISIP 669

Query: 666 WMLLPKPFLLKKQHEERHQGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHT 725
            ML+ KP  +K+ H +  + +   L H  +++D+ E  +  H  EEF+  EVFVHQ+IHT
Sbjct: 670 VMLVIKPLFMKRFHFQ--EVERKKLGHHEEEHDD-EALYTGHHGEEFEMGEVFVHQVIHT 729

Query: 726 IEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATV 785
           IEFVLGAVSNTASYLRLWALSLAHSELSSVF+++IL+      +  +  VG   ++ A+V
Sbjct: 730 IEFVLGAVSNTASYLRLWALSLAHSELSSVFWERILIGQVERGNPFLAFVGFGAWLGASV 789

Query: 786 GVLLVMETLSAFLHALRLHWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 810
            VLL+ME+LSAFLHALRLHWVEFQNKFY GDG +F P+S + +  ED+
Sbjct: 790 AVLLLMESLSAFLHALRLHWVEFQNKFYIGDGVRFIPYSATRILSEDD 814

BLAST of MC08g0714 vs. ExPASy Swiss-Prot
Match: A1A5G6 (V-type proton ATPase 116 kDa subunit a1 OS=Xenopus tropicalis OX=8364 GN=atp6v0a1 PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 2.1e-178
Identity = 359/851 (42.19%), Postives = 514/851 (60.40%), Query Frame = 0

Query: 3   DLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEMA 62
           +L RSE M LAQL + +E+A+  +S LG+LG  QF DLN   + FQR +   ++RC EM 
Sbjct: 3   ELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMD 62

Query: 63  RKLRFFRDQMARAGLS--PSTNSLGVP-GLDLDNLEVKLGELEAELLEINDNNEKLQRNH 122
           RKLRF   ++ +A +S   +  +  VP   D+ +LE    ++E EL EIN N E L+RN 
Sbjct: 63  RKLRFVEKEVKKANISILDTGENPEVPFPRDMIDLEANFEKIEIELKEINTNQEALKRNF 122

Query: 123 SELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQ-- 182
            EL E K +L+K  +FF                    +   D  LL E     +P +   
Sbjct: 123 LELTELKFILRKTQQFF--------------------DEMADPDLLEESSSLLEPSEMGR 182

Query: 183 ---VKLGYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVI 242
              ++LG+++G++ RE+   FER+L+R  RGN++LRQA ++  + DPV+G+ V K+VF+I
Sbjct: 183 GAPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDSVHKSVFII 242

Query: 243 FFSGERAKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKL 302
           FF G++ KN++ KICE F A+ YP  +   ++ +M T V+ ++ +L+  ++  + HR ++
Sbjct: 243 FFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMATGVNTRIEDLQMVLNQTEDHRQRV 302

Query: 303 FQTIGHQYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATY 362
            Q        W + V+K K+IYHTLN+ ++DVT+KCL+ E WCPV   + IQ A+ + T 
Sbjct: 303 LQAAAKSLRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVADLDSIQFALRRGTE 362

Query: 363 DSKSQIEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFL 422
            S S + +I + ++T + PPTY +TNKFT  FQ +VDAYG+  Y+E NP  YTIITFPFL
Sbjct: 363 HSGSTVPSILNRMQTNQTPPTYNKTNKFTYGFQNLVDAYGIGSYREINPAPYTIITFPFL 422

Query: 423 FAVMFGDWGHGICLLLATLYFITREKKFSSEKL-GDIIEMTFGGRYVIMMMALFSIYTGL 482
           FAVMFGD+GHGI + L  ++ + RE +  S+K+  ++  M F GRY+I++M LFSIYTGL
Sbjct: 423 FAVMFGDFGHGILMTLFAVWMVVRESRILSQKIDNELFTMMFSGRYIILLMGLFSIYTGL 482

Query: 483 IYNEFFSVPFELFGPSAYGCR----DTSCRDA---TTIGLIKVRDT------YLFGVDPK 542
           IYN+ FS    LFG S++  R    DT   D    T++  +    T      Y FG+DP 
Sbjct: 483 IYNDCFSKALNLFG-SSWSVRPMFTDTWSEDLLKHTSVLQLNPNVTGVFNGPYPFGIDPI 542

Query: 543 WHGTRSELPFLNSLKMKMSILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNS 602
           W    ++L FLNS KMKMS++LG+  M  G+ LS  N  +F + +NI+  F+P+MIF+ +
Sbjct: 543 WSLATNKLTFLNSFKMKMSVVLGIIHMTFGVALSLLNHIYFKKPLNIYLGFIPEMIFMTT 602

Query: 603 LFGYLSLLIIVKWCSGSQA------DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLL 662
           LFGYL +LII KWC+   +       L    I MFL    D     L+ GQ  LQ  L++
Sbjct: 603 LFGYLVILIIYKWCAYDASTSMVAPSLLIHFINMFLFSYQDTSLPMLYKGQMGLQCFLVV 662

Query: 663 SALIAVPWMLLPKPFLLKKQH-EERHQG-QSYSLIH-----STDDNDETEHSHGSHSHEE 722
            A+I VPWML+ KP +L++Q+   +H G  ++  I      + +D +  +H   S   EE
Sbjct: 663 CAIICVPWMLVVKPLILRRQYLRRKHLGTHNFGGIRVGNGPTEEDAEIIQHDQLSMHSEE 722

Query: 723 ---------FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKILL 782
                    FDF +  VHQ IHTIE+ LG +SNTASYLRLWALSLAH++LS V +  ++ 
Sbjct: 723 GEEPAMEEVFDFGDTVVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMH 782

Query: 783 LAWGFNSLMIRAVGVVV---FICATVGVLLVMETLSAFLHALRLHWVEFQNKFYAGDGFK 807
           +     SL      V V   F   T+ +LL+ME LSAFLHALRLHWVEFQNKFY G GFK
Sbjct: 783 VGLSIRSLGGGIALVFVFSAFATLTIAILLIMEGLSAFLHALRLHWVEFQNKFYMGTGFK 832

BLAST of MC08g0714 vs. NCBI nr
Match: XP_022144381.1 (V-type proton ATPase subunit a2-like [Momordica charantia])

HSP 1 Score: 1586 bits (4106), Expect = 0.0
Identity = 808/808 (100.00%), Postives = 808/808 (100.00%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM
Sbjct: 1   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 60

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE
Sbjct: 61  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 120

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 181
           LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG
Sbjct: 121 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 180

Query: 182 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 241
           YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA
Sbjct: 181 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 240

Query: 242 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 301
           KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ
Sbjct: 241 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 300

Query: 302 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 361
           YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE
Sbjct: 301 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 360

Query: 362 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 421
           AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD
Sbjct: 361 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 420

Query: 422 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 481
           WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV
Sbjct: 421 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 480

Query: 482 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 541
           PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL
Sbjct: 481 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 540

Query: 542 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 601
           LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL
Sbjct: 541 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 600

Query: 602 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 661
           YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG
Sbjct: 601 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 660

Query: 662 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 721
           QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL
Sbjct: 661 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 720

Query: 722 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 781
           SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW
Sbjct: 721 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 780

Query: 782 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           VEFQNKFYAGDGFKFSPFSFSSLRDEDE
Sbjct: 781 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 808

BLAST of MC08g0714 vs. NCBI nr
Match: XP_022951118.1 (V-type proton ATPase subunit a2-like [Cucurbita moschata] >KAG6585562.1 V-type proton ATPase subunit a3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1441 bits (3729), Expect = 0.0
Identity = 728/808 (90.10%), Postives = 766/808 (94.80%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDLLRSEPMQ  QLIIPNES HRT+SYLGDLGLFQFNDLNASRSPFQRTYA  IKRCGEM
Sbjct: 1   MDLLRSEPMQFVQLIIPNESVHRTVSYLGDLGLFQFNDLNASRSPFQRTYAAQIKRCGEM 60

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           ARKLRFFR+QMARAGLSPS+NSL V   DLDNLEVKLGE EAELLEIN NNEKL+RN+SE
Sbjct: 61  ARKLRFFREQMARAGLSPSSNSLAVADFDLDNLEVKLGEFEAELLEINGNNEKLRRNYSE 120

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 181
           LLEYKLVLQKVGEFF LAQSIAAAQQRELEVQQN EGSIDSPLLLEQEMTTD  KQVKLG
Sbjct: 121 LLEYKLVLQKVGEFFHLAQSIAAAQQRELEVQQNGEGSIDSPLLLEQEMTTDSTKQVKLG 180

Query: 182 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 241
           YISGLV REKSMAFERILFRSTRGN+YLRQAVVDGSVTDPVSGEKVEKNVFVIF+SGERA
Sbjct: 181 YISGLVLREKSMAFERILFRSTRGNVYLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGERA 240

Query: 242 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 301
           KNKILKI EAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTID+G++HRSKL Q IGHQ
Sbjct: 241 KNKILKISEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDLGQVHRSKLLQMIGHQ 300

Query: 302 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 361
           YEQWNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATNQIQN + KAT+DSKSQIE
Sbjct: 301 YEQWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATNQIQNVIQKATFDSKSQIE 360

Query: 362 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 421
           AIFHVL+TKEAPPTYFRTNKFTS+FQEIVDAYG+AKYQEANPGVYTI+TFPFLFAVMFGD
Sbjct: 361 AIFHVLQTKEAPPTYFRTNKFTSTFQEIVDAYGIAKYQEANPGVYTIVTFPFLFAVMFGD 420

Query: 422 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 481
           WGHGICLLLATLY I REKKFSS+KLGDI+EMTF GRYVIMMMALFSIYTGLIYNEFFSV
Sbjct: 421 WGHGICLLLATLYLIIREKKFSSQKLGDIVEMTFAGRYVIMMMALFSIYTGLIYNEFFSV 480

Query: 482 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 541
           PFELFGPSAYGCR TSCRDATTIGLIKVRDTY FGVDPKWHGTRSELPFLNSLKMKMSIL
Sbjct: 481 PFELFGPSAYGCRHTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSIL 540

Query: 542 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 601
           LGVAQMNLGIILSYFNAKFF E INIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL
Sbjct: 541 LGVAQMNLGIILSYFNAKFFEEKINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 600

Query: 602 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 661
           YH+MIYMFLSPTDDLGEN+LFPGQ+F+Q++LLL+AL AVPWMLLPKPFLL+KQ+ ERHQG
Sbjct: 601 YHVMIYMFLSPTDDLGENELFPGQQFVQVLLLLAALTAVPWMLLPKPFLLRKQNAERHQG 660

Query: 662 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 721
           QSYSL+H TDDN ET+  H S SH++FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL
Sbjct: 661 QSYSLLHGTDDNLETKQHHSSPSHDDFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 720

Query: 722 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 781
           SLAHSELSSVFYDK+LLLAWGF+S  IR +G+ VFICATVGVLLVMETLSAFLHALRLHW
Sbjct: 721 SLAHSELSSVFYDKVLLLAWGFDSWSIRIIGIAVFICATVGVLLVMETLSAFLHALRLHW 780

Query: 782 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           VEFQNKFYAGDGFKFSP+SFS LR+EDE
Sbjct: 781 VEFQNKFYAGDGFKFSPYSFSLLREEDE 808

BLAST of MC08g0714 vs. NCBI nr
Match: XP_038884965.1 (V-type proton ATPase subunit a2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1439 bits (3725), Expect = 0.0
Identity = 729/808 (90.22%), Postives = 760/808 (94.06%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDLLRSEPMQL QLIIPNESAHR ISYLGDLGLFQFNDLNASRSPFQR YA  IKRCGEM
Sbjct: 1   MDLLRSEPMQLVQLIIPNESAHRMISYLGDLGLFQFNDLNASRSPFQRAYAAQIKRCGEM 60

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           ARKLRFFR++M +AGL PS+N +G P  DLDNLEVKL E E ELLEINDNNEKLQ N+SE
Sbjct: 61  ARKLRFFREKMTKAGLLPSSNPIGAPDSDLDNLEVKLREFEVELLEINDNNEKLQHNYSE 120

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 181
           LLEYKLVLQKVGEFF  AQSIAAAQQRE EVQQN EGSID+PLLLEQEMTTD  KQVKLG
Sbjct: 121 LLEYKLVLQKVGEFFHQAQSIAAAQQREQEVQQNGEGSIDTPLLLEQEMTTDTAKQVKLG 180

Query: 182 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 241
           YISGLVPREKSMAFERILFRSTRGN+YLRQAVVDGSVTDPVSGEKVEKNVFVIF+SGERA
Sbjct: 181 YISGLVPREKSMAFERILFRSTRGNVYLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGERA 240

Query: 242 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 301
           K+KI KICEAFGANRYPFTDDLGKQ QMITEVSGKLSELKTTID+G+ HRSKL Q IG Q
Sbjct: 241 KDKIRKICEAFGANRYPFTDDLGKQFQMITEVSGKLSELKTTIDMGQRHRSKLLQMIGRQ 300

Query: 302 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 361
           YE WNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATNQIQ+ M +AT DSKSQIE
Sbjct: 301 YELWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATNQIQSVMQRATLDSKSQIE 360

Query: 362 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 421
           AIFHVL TKEAPPT+FRTNKFTSSFQEIVDAYGVAKYQEANPGVYTI+TFPFLFAVMFGD
Sbjct: 361 AIFHVLHTKEAPPTFFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMFGD 420

Query: 422 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 481
           WGHGICLLLATLYFI REKKFSS+KLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV
Sbjct: 421 WGHGICLLLATLYFIIREKKFSSQKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 480

Query: 482 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 541
           PFELFGPSAYGCRD+SCRDATTIGLIKV DTY FGVDPKWHGTRSELPFLNSLKMKMSIL
Sbjct: 481 PFELFGPSAYGCRDSSCRDATTIGLIKVHDTYPFGVDPKWHGTRSELPFLNSLKMKMSIL 540

Query: 542 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 601
           LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKW SGSQADL
Sbjct: 541 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWYSGSQADL 600

Query: 602 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 661
           YH+MIYMFLSPTDDLGENQLFPGQKFLQL+LLLSAL AVPWML PKPFLLKKQ+EERHQG
Sbjct: 601 YHVMIYMFLSPTDDLGENQLFPGQKFLQLLLLLSALTAVPWMLFPKPFLLKKQNEERHQG 660

Query: 662 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 721
           QSYS++ STDDN E E  HGSH HEEF+FSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL
Sbjct: 661 QSYSVLQSTDDNQEMEQHHGSHGHEEFEFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 720

Query: 722 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 781
           SLAHSELSSVFYDK+LLLAWGFNSL+IR +G+ VF+CATVGVLLVMETLSAFLHALRLHW
Sbjct: 721 SLAHSELSSVFYDKVLLLAWGFNSLIIRILGIAVFVCATVGVLLVMETLSAFLHALRLHW 780

Query: 782 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           VEFQNKFYAGDGFKFSPFSFS LR+EDE
Sbjct: 781 VEFQNKFYAGDGFKFSPFSFSLLREEDE 808

BLAST of MC08g0714 vs. NCBI nr
Match: XP_023537097.1 (V-type proton ATPase subunit a2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1439 bits (3724), Expect = 0.0
Identity = 726/808 (89.85%), Postives = 766/808 (94.80%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDLLRSEPMQ  QLI+PNES HRT+SYLGDLGLFQFNDLNASRSPFQRTYA  IKRCGEM
Sbjct: 1   MDLLRSEPMQFVQLIVPNESVHRTVSYLGDLGLFQFNDLNASRSPFQRTYAAQIKRCGEM 60

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           ARKLRFFR+QMARAGLSPS+NSL V   DLDNLEVKLGE EAELLEIN NNEKL+RN+SE
Sbjct: 61  ARKLRFFREQMARAGLSPSSNSLAVADFDLDNLEVKLGEFEAELLEINGNNEKLRRNYSE 120

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 181
           LLEYKLVLQKVGEFF LAQSIAAAQQRELEVQQN EGSIDSPLLLEQEMTTD  KQVKLG
Sbjct: 121 LLEYKLVLQKVGEFFHLAQSIAAAQQRELEVQQNGEGSIDSPLLLEQEMTTDSTKQVKLG 180

Query: 182 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 241
           YISGLV REKSMAFERILFRSTRGN+YLRQAVVDGSVTDPVSGEKVEKNVFVIF+SGERA
Sbjct: 181 YISGLVLREKSMAFERILFRSTRGNVYLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGERA 240

Query: 242 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 301
           KNKILKI EAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTID+G++HRSKL Q IGHQ
Sbjct: 241 KNKILKISEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDLGQVHRSKLLQMIGHQ 300

Query: 302 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 361
           YEQWNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATNQIQN + KAT+DSKSQIE
Sbjct: 301 YEQWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATNQIQNVIQKATFDSKSQIE 360

Query: 362 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 421
           AIFHVL+TKEAPPTYFRTNKFTS+FQEIVDAYG+AKYQEANPGVYTI+TFPFLFAVMFGD
Sbjct: 361 AIFHVLQTKEAPPTYFRTNKFTSTFQEIVDAYGIAKYQEANPGVYTIVTFPFLFAVMFGD 420

Query: 422 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 481
           WGHGICLLLATLY I REKKFSS+KLGDI+EMTF GRYVIMMMALFSIYTGLIYNEFFSV
Sbjct: 421 WGHGICLLLATLYLIIREKKFSSQKLGDIVEMTFAGRYVIMMMALFSIYTGLIYNEFFSV 480

Query: 482 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 541
           PFELFGPSAYGCR TSCRDATTIGLIKVRDTY FGVDPKWHGTRSELPFLNSLKMKMSIL
Sbjct: 481 PFELFGPSAYGCRHTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSIL 540

Query: 542 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 601
           LGVAQMNLGIILSYFNAKFF E INIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL
Sbjct: 541 LGVAQMNLGIILSYFNAKFFEEKINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 600

Query: 602 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 661
           YH+MIYMFLSPTDDLGEN+LFPGQ+F+Q++LLL+AL AVPWMLLPKPFLL+KQ+ ERHQG
Sbjct: 601 YHVMIYMFLSPTDDLGENELFPGQQFVQVLLLLAALTAVPWMLLPKPFLLRKQNAERHQG 660

Query: 662 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 721
           +SYSL+H TDDN ET+  H S SH++FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL
Sbjct: 661 RSYSLLHGTDDNLETKQHHSSPSHDDFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 720

Query: 722 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 781
           SLAHSELSSVFYDK+LLLAWGF+S  IR +G+ VFICATVGVLLVMETLSAFLHALRLHW
Sbjct: 721 SLAHSELSSVFYDKVLLLAWGFDSWSIRIIGIAVFICATVGVLLVMETLSAFLHALRLHW 780

Query: 782 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           VEFQNKFYAGDGFKFSP+SFS LR+EDE
Sbjct: 781 VEFQNKFYAGDGFKFSPYSFSLLREEDE 808

BLAST of MC08g0714 vs. NCBI nr
Match: KAA0065191.1 (Vacuolar proton ATPase [Cucumis melo var. makuwa])

HSP 1 Score: 1437 bits (3720), Expect = 0.0
Identity = 730/809 (90.23%), Postives = 762/809 (94.19%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATH-IKRCGE 61
           MDLLRSEPMQL QLIIPNESA RTI YLGDLGLFQFNDLNAS+SPFQRTYA   IKRCGE
Sbjct: 1   MDLLRSEPMQLVQLIIPNESARRTIFYLGDLGLFQFNDLNASKSPFQRTYAAQQIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QMARAGLSPS++SLG P  DLDNLEVKLGE E ELLEI DNNEKLQRN+S
Sbjct: 61  MARKLRFFREQMARAGLSPSSHSLGTPDFDLDNLEVKLGEFEVELLEIKDNNEKLQRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVL+KVGEFF LAQSIAAA QRELEVQQN EG ID+PLLLEQEMTTDP KQVKL
Sbjct: 121 ELLEYKLVLEKVGEFFHLAQSIAAAHQRELEVQQNGEGYIDTPLLLEQEMTTDPTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLVPREKSMAFERILFRSTRGN+YLRQAV+DGSVTDPVSG+KVEKNVFVIF+SGER
Sbjct: 181 GYISGLVPREKSMAFERILFRSTRGNVYLRQAVIDGSVTDPVSGDKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK KI KICEAFGANRYPFTDDLGKQ QMITEVS KLSELKTTID+G+LH S+L QTIGH
Sbjct: 241 AKEKIRKICEAFGANRYPFTDDLGKQFQMITEVSRKLSELKTTIDMGQLHGSQLLQTIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQI 361
           QYE WNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATNQIQ+ M KAT DSKSQI
Sbjct: 301 QYELWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATNQIQSVMQKATLDSKSQI 360

Query: 362 EAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFG 421
           EAIFHVL TKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTI+TFPFLFAVMFG
Sbjct: 361 EAIFHVLDTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMFG 420

Query: 422 DWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFS 481
           DWGHGICLLLATLYFI REKKFS +KLGDI+EMTFGGRYVIMMMALFSIYTGLIYNEFFS
Sbjct: 421 DWGHGICLLLATLYFIIREKKFSGQKLGDIVEMTFGGRYVIMMMALFSIYTGLIYNEFFS 480

Query: 482 VPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSI 541
           VPFELFGPSAYGCRDTSCRDAT+IGL+KVRDTY FGVDPKWHGTRSELPFLNSLKMKMSI
Sbjct: 481 VPFELFGPSAYGCRDTSCRDATSIGLVKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSI 540

Query: 542 LLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQAD 601
           LLGVAQMNLGIILSYFNAKFFGE+INIWYQFVPQMIFLNSLFGYLSLLIIVKW SGSQAD
Sbjct: 541 LLGVAQMNLGIILSYFNAKFFGESINIWYQFVPQMIFLNSLFGYLSLLIIVKWYSGSQAD 600

Query: 602 LYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQ 661
           LYH+MIYMFLSPTDDLGENQLFPGQKFLQL+LLLSAL AVPWML PKP LLKKQ+EERHQ
Sbjct: 601 LYHVMIYMFLSPTDDLGENQLFPGQKFLQLLLLLSALTAVPWMLFPKPLLLKKQNEERHQ 660

Query: 662 GQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWA 721
           GQSYS++H TDDN E E  HGSH HEEFD SEVFVHQLIHTIEFVLGAVSNTASYLRLWA
Sbjct: 661 GQSYSVLHCTDDNHEIERHHGSHGHEEFDLSEVFVHQLIHTIEFVLGAVSNTASYLRLWA 720

Query: 722 LSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLH 781
           LSLAHSELSSVFYDK+LLLAWGFNSL+IR VG+ VFI ATVGVLL+METLSAFLHALRLH
Sbjct: 721 LSLAHSELSSVFYDKVLLLAWGFNSLIIRIVGMAVFIFATVGVLLIMETLSAFLHALRLH 780

Query: 782 WVEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           WVEFQNKFYAGDGFKFSPFSFS LR+EDE
Sbjct: 781 WVEFQNKFYAGDGFKFSPFSFSLLREEDE 809

BLAST of MC08g0714 vs. ExPASy TrEMBL
Match: A0A6J1CT88 (V-type proton ATPase subunit a OS=Momordica charantia OX=3673 GN=LOC111014074 PE=3 SV=1)

HSP 1 Score: 1586 bits (4106), Expect = 0.0
Identity = 808/808 (100.00%), Postives = 808/808 (100.00%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM
Sbjct: 1   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 60

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE
Sbjct: 61  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 120

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 181
           LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG
Sbjct: 121 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 180

Query: 182 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 241
           YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA
Sbjct: 181 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 240

Query: 242 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 301
           KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ
Sbjct: 241 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 300

Query: 302 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 361
           YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE
Sbjct: 301 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 360

Query: 362 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 421
           AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD
Sbjct: 361 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 420

Query: 422 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 481
           WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV
Sbjct: 421 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 480

Query: 482 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 541
           PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL
Sbjct: 481 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 540

Query: 542 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 601
           LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL
Sbjct: 541 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 600

Query: 602 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 661
           YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG
Sbjct: 601 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 660

Query: 662 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 721
           QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL
Sbjct: 661 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 720

Query: 722 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 781
           SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW
Sbjct: 721 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 780

Query: 782 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           VEFQNKFYAGDGFKFSPFSFSSLRDEDE
Sbjct: 781 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 808

BLAST of MC08g0714 vs. ExPASy TrEMBL
Match: A0A6J1GGT7 (V-type proton ATPase subunit a OS=Cucurbita moschata OX=3662 GN=LOC111454061 PE=3 SV=1)

HSP 1 Score: 1441 bits (3729), Expect = 0.0
Identity = 728/808 (90.10%), Postives = 766/808 (94.80%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDLLRSEPMQ  QLIIPNES HRT+SYLGDLGLFQFNDLNASRSPFQRTYA  IKRCGEM
Sbjct: 1   MDLLRSEPMQFVQLIIPNESVHRTVSYLGDLGLFQFNDLNASRSPFQRTYAAQIKRCGEM 60

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           ARKLRFFR+QMARAGLSPS+NSL V   DLDNLEVKLGE EAELLEIN NNEKL+RN+SE
Sbjct: 61  ARKLRFFREQMARAGLSPSSNSLAVADFDLDNLEVKLGEFEAELLEINGNNEKLRRNYSE 120

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 181
           LLEYKLVLQKVGEFF LAQSIAAAQQRELEVQQN EGSIDSPLLLEQEMTTD  KQVKLG
Sbjct: 121 LLEYKLVLQKVGEFFHLAQSIAAAQQRELEVQQNGEGSIDSPLLLEQEMTTDSTKQVKLG 180

Query: 182 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 241
           YISGLV REKSMAFERILFRSTRGN+YLRQAVVDGSVTDPVSGEKVEKNVFVIF+SGERA
Sbjct: 181 YISGLVLREKSMAFERILFRSTRGNVYLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGERA 240

Query: 242 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 301
           KNKILKI EAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTID+G++HRSKL Q IGHQ
Sbjct: 241 KNKILKISEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDLGQVHRSKLLQMIGHQ 300

Query: 302 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 361
           YEQWNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATNQIQN + KAT+DSKSQIE
Sbjct: 301 YEQWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATNQIQNVIQKATFDSKSQIE 360

Query: 362 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 421
           AIFHVL+TKEAPPTYFRTNKFTS+FQEIVDAYG+AKYQEANPGVYTI+TFPFLFAVMFGD
Sbjct: 361 AIFHVLQTKEAPPTYFRTNKFTSTFQEIVDAYGIAKYQEANPGVYTIVTFPFLFAVMFGD 420

Query: 422 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 481
           WGHGICLLLATLY I REKKFSS+KLGDI+EMTF GRYVIMMMALFSIYTGLIYNEFFSV
Sbjct: 421 WGHGICLLLATLYLIIREKKFSSQKLGDIVEMTFAGRYVIMMMALFSIYTGLIYNEFFSV 480

Query: 482 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 541
           PFELFGPSAYGCR TSCRDATTIGLIKVRDTY FGVDPKWHGTRSELPFLNSLKMKMSIL
Sbjct: 481 PFELFGPSAYGCRHTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSIL 540

Query: 542 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 601
           LGVAQMNLGIILSYFNAKFF E INIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL
Sbjct: 541 LGVAQMNLGIILSYFNAKFFEEKINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 600

Query: 602 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 661
           YH+MIYMFLSPTDDLGEN+LFPGQ+F+Q++LLL+AL AVPWMLLPKPFLL+KQ+ ERHQG
Sbjct: 601 YHVMIYMFLSPTDDLGENELFPGQQFVQVLLLLAALTAVPWMLLPKPFLLRKQNAERHQG 660

Query: 662 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 721
           QSYSL+H TDDN ET+  H S SH++FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL
Sbjct: 661 QSYSLLHGTDDNLETKQHHSSPSHDDFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 720

Query: 722 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 781
           SLAHSELSSVFYDK+LLLAWGF+S  IR +G+ VFICATVGVLLVMETLSAFLHALRLHW
Sbjct: 721 SLAHSELSSVFYDKVLLLAWGFDSWSIRIIGIAVFICATVGVLLVMETLSAFLHALRLHW 780

Query: 782 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           VEFQNKFYAGDGFKFSP+SFS LR+EDE
Sbjct: 781 VEFQNKFYAGDGFKFSPYSFSLLREEDE 808

BLAST of MC08g0714 vs. ExPASy TrEMBL
Match: A0A5A7VHH1 (V-type proton ATPase subunit a OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005280 PE=3 SV=1)

HSP 1 Score: 1437 bits (3720), Expect = 0.0
Identity = 730/809 (90.23%), Postives = 762/809 (94.19%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATH-IKRCGE 61
           MDLLRSEPMQL QLIIPNESA RTI YLGDLGLFQFNDLNAS+SPFQRTYA   IKRCGE
Sbjct: 1   MDLLRSEPMQLVQLIIPNESARRTIFYLGDLGLFQFNDLNASKSPFQRTYAAQQIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QMARAGLSPS++SLG P  DLDNLEVKLGE E ELLEI DNNEKLQRN+S
Sbjct: 61  MARKLRFFREQMARAGLSPSSHSLGTPDFDLDNLEVKLGEFEVELLEIKDNNEKLQRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVL+KVGEFF LAQSIAAA QRELEVQQN EG ID+PLLLEQEMTTDP KQVKL
Sbjct: 121 ELLEYKLVLEKVGEFFHLAQSIAAAHQRELEVQQNGEGYIDTPLLLEQEMTTDPTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLVPREKSMAFERILFRSTRGN+YLRQAV+DGSVTDPVSG+KVEKNVFVIF+SGER
Sbjct: 181 GYISGLVPREKSMAFERILFRSTRGNVYLRQAVIDGSVTDPVSGDKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK KI KICEAFGANRYPFTDDLGKQ QMITEVS KLSELKTTID+G+LH S+L QTIGH
Sbjct: 241 AKEKIRKICEAFGANRYPFTDDLGKQFQMITEVSRKLSELKTTIDMGQLHGSQLLQTIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQI 361
           QYE WNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATNQIQ+ M KAT DSKSQI
Sbjct: 301 QYELWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATNQIQSVMQKATLDSKSQI 360

Query: 362 EAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFG 421
           EAIFHVL TKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTI+TFPFLFAVMFG
Sbjct: 361 EAIFHVLDTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMFG 420

Query: 422 DWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFS 481
           DWGHGICLLLATLYFI REKKFS +KLGDI+EMTFGGRYVIMMMALFSIYTGLIYNEFFS
Sbjct: 421 DWGHGICLLLATLYFIIREKKFSGQKLGDIVEMTFGGRYVIMMMALFSIYTGLIYNEFFS 480

Query: 482 VPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSI 541
           VPFELFGPSAYGCRDTSCRDAT+IGL+KVRDTY FGVDPKWHGTRSELPFLNSLKMKMSI
Sbjct: 481 VPFELFGPSAYGCRDTSCRDATSIGLVKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSI 540

Query: 542 LLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQAD 601
           LLGVAQMNLGIILSYFNAKFFGE+INIWYQFVPQMIFLNSLFGYLSLLIIVKW SGSQAD
Sbjct: 541 LLGVAQMNLGIILSYFNAKFFGESINIWYQFVPQMIFLNSLFGYLSLLIIVKWYSGSQAD 600

Query: 602 LYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQ 661
           LYH+MIYMFLSPTDDLGENQLFPGQKFLQL+LLLSAL AVPWML PKP LLKKQ+EERHQ
Sbjct: 601 LYHVMIYMFLSPTDDLGENQLFPGQKFLQLLLLLSALTAVPWMLFPKPLLLKKQNEERHQ 660

Query: 662 GQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWA 721
           GQSYS++H TDDN E E  HGSH HEEFD SEVFVHQLIHTIEFVLGAVSNTASYLRLWA
Sbjct: 661 GQSYSVLHCTDDNHEIERHHGSHGHEEFDLSEVFVHQLIHTIEFVLGAVSNTASYLRLWA 720

Query: 722 LSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLH 781
           LSLAHSELSSVFYDK+LLLAWGFNSL+IR VG+ VFI ATVGVLL+METLSAFLHALRLH
Sbjct: 721 LSLAHSELSSVFYDKVLLLAWGFNSLIIRIVGMAVFIFATVGVLLIMETLSAFLHALRLH 780

Query: 782 WVEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           WVEFQNKFYAGDGFKFSPFSFS LR+EDE
Sbjct: 781 WVEFQNKFYAGDGFKFSPFSFSLLREEDE 809

BLAST of MC08g0714 vs. ExPASy TrEMBL
Match: A0A0A0LLK1 (V-type proton ATPase subunit a OS=Cucumis sativus OX=3659 GN=Csa_2G364570 PE=3 SV=1)

HSP 1 Score: 1437 bits (3720), Expect = 0.0
Identity = 730/808 (90.35%), Postives = 761/808 (94.18%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDLLRSEPMQL QLIIPNESA RTISYLGDLGLFQFNDLNAS+SPFQRTYA  IKRCGEM
Sbjct: 1   MDLLRSEPMQLVQLIIPNESARRTISYLGDLGLFQFNDLNASKSPFQRTYAAQIKRCGEM 60

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           ARKLRFFR+QM RAGLSPS+ SLG    DLDNLEVKLGELE ELLEI DNNEKLQRN+SE
Sbjct: 61  ARKLRFFREQMTRAGLSPSSYSLGTHDFDLDNLEVKLGELEVELLEIKDNNEKLQRNYSE 120

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 181
           LLEYKLVLQKVGEFF LAQ  AAA QRELEVQQN EGSID+PLLLEQEMTTDP KQVKLG
Sbjct: 121 LLEYKLVLQKVGEFFHLAQRTAAAHQRELEVQQNGEGSIDTPLLLEQEMTTDPTKQVKLG 180

Query: 182 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 241
           YISGLVPREKSMAFERILFRSTRGN+YLRQAV+DGSVTDPVSG+KVEKNVFVIF+SGERA
Sbjct: 181 YISGLVPREKSMAFERILFRSTRGNVYLRQAVIDGSVTDPVSGDKVEKNVFVIFYSGERA 240

Query: 242 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 301
           K KI KICEAFGANRYPFTDDLGKQ QMITEVS KLSELK TID+G+LHRS+L QTIGHQ
Sbjct: 241 KEKIRKICEAFGANRYPFTDDLGKQFQMITEVSRKLSELKITIDMGQLHRSQLLQTIGHQ 300

Query: 302 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 361
           YE WNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV AT QIQ+ M KAT+DSKSQIE
Sbjct: 301 YELWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATIQIQSVMQKATHDSKSQIE 360

Query: 362 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 421
           AIFHVL TKEAPPTYF TNKFTSSFQEIVDAYGVAKYQEANPGVYTI+TFPFLFAVMFGD
Sbjct: 361 AIFHVLDTKEAPPTYFCTNKFTSSFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMFGD 420

Query: 422 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 481
           WGHGICLLLATLYFI REKKFS +KLGDI+EMTFGGRYVIMMMALFSIYTGLIYNEFFSV
Sbjct: 421 WGHGICLLLATLYFIIREKKFSGQKLGDIVEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 480

Query: 482 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 541
           PFELFGPSAYGCRDTSCRDAT+IGLIKVRDTY FGVDPKWHGTRSELPFLNSLKMKMSIL
Sbjct: 481 PFELFGPSAYGCRDTSCRDATSIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSIL 540

Query: 542 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 601
           LGVAQMNLGIILSYFNAKFFGE+INIWYQFVPQMIFLNSLFGYLSLLIIVKW SGSQADL
Sbjct: 541 LGVAQMNLGIILSYFNAKFFGESINIWYQFVPQMIFLNSLFGYLSLLIIVKWYSGSQADL 600

Query: 602 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 661
           YH+MIYMFLSPTDDLGENQLFPGQKFLQL+LLLSAL AVPWML PKPFLLKKQ+EERHQG
Sbjct: 601 YHVMIYMFLSPTDDLGENQLFPGQKFLQLLLLLSALTAVPWMLFPKPFLLKKQNEERHQG 660

Query: 662 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 721
           QSYS++H TDDN E E  HGSH HEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL
Sbjct: 661 QSYSVLHCTDDNHEIERHHGSHGHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 720

Query: 722 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 781
           SLAHSELSSVFYDK+LLLAWGF+SL+IR VG+ VFI ATVGVLL+METLSAFLHALRLHW
Sbjct: 721 SLAHSELSSVFYDKVLLLAWGFDSLIIRIVGMAVFIFATVGVLLIMETLSAFLHALRLHW 780

Query: 782 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           VEFQNKFYAGDGFKFSPFSFS LR+EDE
Sbjct: 781 VEFQNKFYAGDGFKFSPFSFSLLREEDE 808

BLAST of MC08g0714 vs. ExPASy TrEMBL
Match: A0A6J1KIY1 (V-type proton ATPase subunit a OS=Cucurbita maxima OX=3661 GN=LOC111496148 PE=3 SV=1)

HSP 1 Score: 1433 bits (3710), Expect = 0.0
Identity = 725/808 (89.73%), Postives = 764/808 (94.55%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDLLRSEPMQ  QLIIPNES HRT+SYLGDLGLFQFNDLNASRSPFQRTYA  IKRCGEM
Sbjct: 1   MDLLRSEPMQFVQLIIPNESVHRTLSYLGDLGLFQFNDLNASRSPFQRTYAAQIKRCGEM 60

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           ARKLRFFR+QMARAGLSPS+NS  V   DLDNLEVKLGE EAELLEIN NNEKL+RN+SE
Sbjct: 61  ARKLRFFREQMARAGLSPSSNSQAVADFDLDNLEVKLGEFEAELLEINGNNEKLRRNYSE 120

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKLG 181
           LLEYKLVLQKVGEFF +AQSIAAAQQRELEVQQN EGSIDSPLLLEQEMTTD  KQVKLG
Sbjct: 121 LLEYKLVLQKVGEFFHIAQSIAAAQQRELEVQQNGEGSIDSPLLLEQEMTTDSTKQVKLG 180

Query: 182 YISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGERA 241
           YISGLV REKSMAFERILFRSTRGN++LRQAVVDGSVTDPVSGEKVEKNVFVIF+SGERA
Sbjct: 181 YISGLVLREKSMAFERILFRSTRGNVHLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGERA 240

Query: 242 KNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGHQ 301
           KNKILKI EAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTID+G++HRSKL Q IGHQ
Sbjct: 241 KNKILKISEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDLGQVHRSKLLQMIGHQ 300

Query: 302 YEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQIE 361
           YEQWNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATNQIQ+ + KAT+DSKSQIE
Sbjct: 301 YEQWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATNQIQSVIQKATFDSKSQIE 360

Query: 362 AIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFGD 421
           AIFHVL+TKEAPPTYFRTNKFTS+FQEIVDAYG+AKYQEANPGVYTI+TFPFLFAVMFGD
Sbjct: 361 AIFHVLQTKEAPPTYFRTNKFTSTFQEIVDAYGIAKYQEANPGVYTIVTFPFLFAVMFGD 420

Query: 422 WGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFSV 481
           WGHGICLLLATLY I REKKFSS+KLGDI+EMTF GRYVIMMMALFSIYTGLIYNEFFSV
Sbjct: 421 WGHGICLLLATLYLIIREKKFSSQKLGDIVEMTFAGRYVIMMMALFSIYTGLIYNEFFSV 480

Query: 482 PFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSIL 541
           PFELFG SAYGCR TSCRDATTIGLIKVRDTY FGVDPKWHGTRSELPFLNSLKMKMSIL
Sbjct: 481 PFELFGRSAYGCRHTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSIL 540

Query: 542 LGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 601
           LGVAQMNLGIILSYFNAKFF E INIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL
Sbjct: 541 LGVAQMNLGIILSYFNAKFFEEKINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQADL 600

Query: 602 YHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQG 661
           YH+MIYMFLSPTDDLGEN+LFPGQ+F+Q++LLL+AL AVPWMLLPKPFLL+KQ+ ERHQG
Sbjct: 601 YHVMIYMFLSPTDDLGENELFPGQQFVQVLLLLAALTAVPWMLLPKPFLLRKQNAERHQG 660

Query: 662 QSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 721
           QSYSL+H TDDN ET+  HGS SH +FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL
Sbjct: 661 QSYSLLHGTDDNLETKQHHGSPSHNDFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWAL 720

Query: 722 SLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLHW 781
           SLAHSELSSVFYDK+LLLAWGFNS  IR +G+ VFICATVGVLLVMETLSAFLHALRLHW
Sbjct: 721 SLAHSELSSVFYDKVLLLAWGFNSWSIRIIGIAVFICATVGVLLVMETLSAFLHALRLHW 780

Query: 782 VEFQNKFYAGDGFKFSPFSFSSLRDEDE 809
           VEFQNKFYAGDGFKFSP+SFS LR+EDE
Sbjct: 781 VEFQNKFYAGDGFKFSPYSFSLLREEDE 808

BLAST of MC08g0714 vs. TAIR 10
Match: AT2G21410.1 (vacuolar proton ATPase A2 )

HSP 1 Score: 1221.1 bits (3158), Expect = 0.0e+00
Identity = 595/809 (73.55%), Postives = 704/809 (87.02%), Query Frame = 0

Query: 1   PMDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGE 60
           PMDL+RSEPMQL Q+I+P ESAH T+SYLGDLGL QF DLN+ +SPFQRTYA  IKRCGE
Sbjct: 14  PMDLMRSEPMQLVQVIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAAQIKRCGE 73

Query: 61  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 120
           MARK+RFF++QM++AG++P         +DLD++EVKL ELEAEL+EIN NN+KLQR+++
Sbjct: 74  MARKIRFFKEQMSKAGVTPKETLDRENDIDLDDVEVKLEELEAELVEINANNDKLQRSYN 133

Query: 121 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 180
           EL+EYKLVL+K GEFF  A   A AQQ E+E +Q  E  +++P LL++E + DP KQVKL
Sbjct: 134 ELVEYKLVLEKAGEFFASAHRSATAQQSEIETEQVGEDLLEAP-LLQEEKSVDPTKQVKL 193

Query: 181 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 240
           G+++GLVPREKSM FERILFR+TRGN+++RQ+V++ SV DP SGEK EKNVFV+F+SGER
Sbjct: 194 GFLTGLVPREKSMVFERILFRATRGNIFIRQSVIEESVVDPNSGEKAEKNVFVVFYSGER 253

Query: 241 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 300
           AK+KILKICEAFGANRYPF++DLGKQ QM+TEVSG+LSELKTTI  G   R+ L +TIG 
Sbjct: 254 AKSKILKICEAFGANRYPFSEDLGKQAQMMTEVSGRLSELKTTIGAGLDQRNILLETIGD 313

Query: 301 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQI 360
           ++EQWNL ++KEK+IYHTLNMLS+DVTKKCLVGEGW PV A  +IQ+A+H+A  DS SQ+
Sbjct: 314 KFEQWNLKIRKEKAIYHTLNMLSLDVTKKCLVGEGWSPVFAATEIQDALHRAAVDSNSQV 373

Query: 361 EAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFG 420
            +IF VLRTKE PPT+FRTNKFT++FQEIVDAYGVAKYQEANP V+TI+TFPFLFAVMFG
Sbjct: 374 GSIFQVLRTKEMPPTFFRTNKFTTAFQEIVDAYGVAKYQEANPSVFTIVTFPFLFAVMFG 433

Query: 421 DWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFS 480
           DWGHGICLLLAT+Y I REKK SS+KLGDI+EM FGGRYVI MM+LFSIYTGLIYNEFFS
Sbjct: 434 DWGHGICLLLATMYLILREKKLSSQKLGDIMEMAFGGRYVIFMMSLFSIYTGLIYNEFFS 493

Query: 481 VPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSI 540
           +P+ LF  SAY CRD SC +ATTIGLIK RDTY FGVDP WHGTRSELPFLNSLKMKMSI
Sbjct: 494 IPYPLFASSAYDCRDVSCSEATTIGLIKTRDTYPFGVDPVWHGTRSELPFLNSLKMKMSI 553

Query: 541 LLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQAD 600
           L+GVAQMNLGII+S+FNAKFF   +NIW+QFVPQMIFLN LFGYLS+LII+KWC+GSQAD
Sbjct: 554 LIGVAQMNLGIIMSFFNAKFFKSAVNIWFQFVPQMIFLNCLFGYLSVLIIIKWCTGSQAD 613

Query: 601 LYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQ 660
           LYH+MIYMFLSP DDLGENQLFP QK +QL  L  AL++VPWMLLPKPF+LKKQHE RHQ
Sbjct: 614 LYHVMIYMFLSPMDDLGENQLFPNQKIVQLTFLFLALVSVPWMLLPKPFILKKQHEARHQ 673

Query: 661 GQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWA 720
           G SY+ +  TD++ + E + G H HEEF+FSE+FVHQLIHTIEFVLGAVSNTASYLRLWA
Sbjct: 674 GLSYAQLDETDESLQVETNGGGHGHEEFEFSEIFVHQLIHTIEFVLGAVSNTASYLRLWA 733

Query: 721 LSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRLH 780
           LSLAHSELSSVFY+K+LL+AWGFN++ I  VG++VFI ATVGVLLVMETLSAFLHALRLH
Sbjct: 734 LSLAHSELSSVFYEKVLLMAWGFNNVFIWIVGILVFIFATVGVLLVMETLSAFLHALRLH 793

Query: 781 WVEFQNKFYAGDGFKFSPFSFSSLRDEDE 810
           WVE+QNKFY GDG+KF+PF+F+ + +EDE
Sbjct: 794 WVEYQNKFYEGDGYKFAPFTFTLVGNEDE 821

BLAST of MC08g0714 vs. TAIR 10
Match: AT4G39080.1 (vacuolar proton ATPase A3 )

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 602/810 (74.32%), Postives = 704/810 (86.91%), Query Frame = 0

Query: 1   PMDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGE 60
           PMDL+RSE MQL QLI+P ESAH T+SYLGDLGL QF DLN+ +SPFQRTYA  IKRCGE
Sbjct: 13  PMDLMRSETMQLVQLIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAAQIKRCGE 72

Query: 61  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 120
           MARK+RFFRDQM++AG+           +DLD++EVKLGELEAEL+EIN NN+KLQR+++
Sbjct: 73  MARKIRFFRDQMSKAGVPAKEMQGKENDIDLDDVEVKLGELEAELVEINANNDKLQRSYN 132

Query: 121 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 180
           EL+EYKLVLQK GEFF  A   AA QQRE E QQ  E  ++SP LL++E + D  KQVKL
Sbjct: 133 ELMEYKLVLQKAGEFFSSAHRSAADQQRETESQQAGEDLLESP-LLQEEKSIDSTKQVKL 192

Query: 181 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 240
           G+++GLVPREKSM FERILFR+TRGN+++RQ V++  V DP SGEK EKNVFV+F+SGER
Sbjct: 193 GFLTGLVPREKSMVFERILFRATRGNIFIRQTVIEEPVIDPNSGEKAEKNVFVVFYSGER 252

Query: 241 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 300
           AK+KILKICEAFGANRYPF++DLG+Q QMITEVSG+LSELKTTID G   R+ L QTIG 
Sbjct: 253 AKSKILKICEAFGANRYPFSEDLGRQAQMITEVSGRLSELKTTIDAGLGQRNILLQTIGD 312

Query: 301 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQI 360
           ++E WNL V+KEK+IYHTLNMLS+DVTKKCLV EGW PV A+ +IQ+A+ +A  DS SQ+
Sbjct: 313 KFELWNLKVRKEKAIYHTLNMLSLDVTKKCLVAEGWSPVFASREIQDALQRAAVDSNSQV 372

Query: 361 EAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFG 420
            +IF VLRTKE+PPTYFRTNKFTS+ QEIVDAYGVAKYQEANPGV+TI+TFPFLFAVMFG
Sbjct: 373 GSIFQVLRTKESPPTYFRTNKFTSAIQEIVDAYGVAKYQEANPGVFTIVTFPFLFAVMFG 432

Query: 421 DWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFS 480
           DWGHGIC+LLAT+Y I +EKK +S+KLGDI+EM FGGRYVI+MM+LFSIYTGLIYNEFFS
Sbjct: 433 DWGHGICILLATMYLILKEKKLASQKLGDIMEMAFGGRYVILMMSLFSIYTGLIYNEFFS 492

Query: 481 VPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSI 540
           +PF LF PSAY CRD SC +ATTIGLIKVRDTY FG+DP WHG+RSELPFLNSLKMKMSI
Sbjct: 493 IPFPLFAPSAYDCRDVSCSEATTIGLIKVRDTYPFGLDPVWHGSRSELPFLNSLKMKMSI 552

Query: 541 LLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQAD 600
           LLGV+QMNLGII+SYFNA+FF  ++NIW+QF+PQMIFLNSLFGYLS+LII+KWC+GSQAD
Sbjct: 553 LLGVSQMNLGIIMSYFNARFFKSSVNIWFQFIPQMIFLNSLFGYLSVLIIIKWCTGSQAD 612

Query: 601 LYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQ 660
           LYH+MIYMFLSP D+LGENQLFP QK LQL+LL  AL++VP MLLPKPF+LKKQHE RHQ
Sbjct: 613 LYHVMIYMFLSPMDELGENQLFPHQKTLQLVLLFLALVSVPCMLLPKPFILKKQHEARHQ 672

Query: 661 GQSYSLIHSTDDNDETE-HSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720
           GQ+Y+ +  TD++   E +  GSH HEEF+FSE+FVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 673 GQAYAPLDETDESLHVETNGGGSHGHEEFEFSEIFVHQLIHTIEFVLGAVSNTASYLRLW 732

Query: 721 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 780
           ALSLAHSELSSVFY+K+LLLAWG+N+ +I  VGV+VFI ATVGVLLVMETLSAFLHALRL
Sbjct: 733 ALSLAHSELSSVFYEKVLLLAWGYNNPLILIVGVLVFIFATVGVLLVMETLSAFLHALRL 792

Query: 781 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 810
           HWVEFQNKFY GDG+KF+PF+F    +EDE
Sbjct: 793 HWVEFQNKFYEGDGYKFAPFTFIFTANEDE 821

BLAST of MC08g0714 vs. TAIR 10
Match: AT2G28520.1 (vacuolar proton ATPase A1 )

HSP 1 Score: 1023.8 bits (2646), Expect = 7.3e-299
Identity = 503/805 (62.48%), Postives = 634/805 (78.76%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHIKRCGEM 61
           MDL+RSE M L QLIIP ESAHR+I+YLG+LGL QF DLNA +SPFQRT+A  +KRCGEM
Sbjct: 11  MDLMRSEKMTLVQLIIPVESAHRSITYLGELGLLQFRDLNADKSPFQRTFANQVKRCGEM 70

Query: 62  ARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHSE 121
           +RKLRFF+DQ+ +AGL  S      P + L +LE +L + E E+LE+N N+EKL++ ++E
Sbjct: 71  SRKLRFFKDQIDKAGLRCSPRLEIEPDIALGDLERQLADHEHEVLEMNSNSEKLRQTYNE 130

Query: 122 LLEYKLVLQKVGEFFQLAQSIAAAQQREL-EVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           LLE+K+VL+K   F   + + A  ++ EL E   +  G I++  LLEQEM      Q  L
Sbjct: 131 LLEFKIVLEKASGFLVSSNTHAIGEEIELHESTYSNNGFIETASLLEQEMNPGHSNQSGL 190

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
            +ISG++ ++K + FER+LFR+TRGNM   Q   D  + DP + E VEK VFV+FFSGE+
Sbjct: 191 RFISGIINKDKLLKFERMLFRATRGNMLFNQTTSDEEIMDPSTSEMVEKVVFVVFFSGEQ 250

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           A+ KILKICEAFGAN YP  +D  KQ Q+  EV  +LS+L+ T+D G  HR+    ++G+
Sbjct: 251 ARTKILKICEAFGANCYPVPEDTTKQRQLTREVLSRLSDLEATLDAGTRHRNNALNSVGY 310

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQIQNAMHKATYDSKSQI 361
               W   V++EK++Y TLNML+ DVTKKCLVGEGWCP  A  QI   + +AT+DS SQ+
Sbjct: 311 SLTNWITTVRREKAVYDTLNMLNFDVTKKCLVGEGWCPTFAKTQIHEVLQRATFDSSSQV 370

Query: 362 EAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMFG 421
             IFHV++  E+PPTYFRTNK T++FQEI+DAYGVA+YQEANP VY+++T+PFLFAVMFG
Sbjct: 371 GVIFHVMQAVESPPTYFRTNKLTNAFQEIIDAYGVARYQEANPAVYSVVTYPFLFAVMFG 430

Query: 422 DWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFFS 481
           DWGHG+CLLL  LY + RE+K S++KLG  +EM FGGRYVI++MALFSIY GLIYNEFFS
Sbjct: 431 DWGHGLCLLLGALYLLARERKLSTQKLGSFMEMLFGGRYVILLMALFSIYCGLIYNEFFS 490

Query: 482 VPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMSI 541
           VPF +FG SAY CRDT+C DA T+GLIK RD Y FGVDP W G+R+ELP+LNSLKMKMSI
Sbjct: 491 VPFHIFGGSAYKCRDTTCSDAYTVGLIKYRDPYPFGVDPSWRGSRTELPYLNSLKMKMSI 550

Query: 542 LLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQAD 601
           LLG+AQMNLG+ILS+FNA+FFG +++I YQF+PQMIFLNSLFGYLSLLII+KWC+GSQAD
Sbjct: 551 LLGIAQMNLGLILSFFNARFFGSSLDIRYQFIPQMIFLNSLFGYLSLLIIIKWCTGSQAD 610

Query: 602 LYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERHQ 661
           LYH+MIYMFLSPT++LGEN+LF GQ+ LQ++LLL A IAVPWML PKPF L+K H ER Q
Sbjct: 611 LYHVMIYMFLSPTEELGENELFWGQRPLQIVLLLLAFIAVPWMLFPKPFALRKIHMERFQ 670

Query: 662 GQSYSLIHSTD---DNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLR 721
           G++Y ++ S++   D +      G H  EEF+FSE+FVHQLIH+IEFVLG+VSNTASYLR
Sbjct: 671 GRTYGVLVSSEVDLDVEPDSARGGGHHEEEFNFSEIFVHQLIHSIEFVLGSVSNTASYLR 730

Query: 722 LWALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHAL 781
           LWALSLAHSELS+VFY+K+LLLAWG+ +++IR +GV VF  AT  +LL+METLSAFLHAL
Sbjct: 731 LWALSLAHSELSTVFYEKVLLLAWGYENILIRLIGVAVFAFATAFILLMMETLSAFLHAL 790

Query: 782 RLHWVEFQNKFYAGDGFKFSPFSFS 803
           RLHWVEF  KF+ GDG+KF PFSF+
Sbjct: 791 RLHWVEFMGKFFNGDGYKFKPFSFA 815