MC08g0613 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0613
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionBRI1 kinase inhibitor 1-like
LocationMC08: 5016922 .. 5017746 (-)
RNA-Seq ExpressionMC08g0613
SyntenyMC08g0613
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGGCAAAGAAAGGCAATTGGAGAAGCAACGATTGGCAGCTTCTTCATCACCTCCTTCAGCATCCTCATCTCCCTCTCATGAATTTTCTTTCTCAGTTTCTCTGCAAACTTCCTCCACAGACGAAACCAAAGCTCCGCCTTCCTTCACCATTGATTTGTCTCCAGCGGATGACATCTTCTTCCATGGCCATTTGCTTCCTCTCCACCTCCTCTCTCATCTGCCAGTCCCTCCCCGCTGCTCAACCAACTCCATTGAAAGCTATGCCCTTCCCATTAGAGACCTGCTAAGTGAAAAGGAACCCAAGAAAGAGAGCAGCAACTTCATCAATTGCAAGAGCAACGAACACTATCAAAACAGCAACAGTGATGATACAAGAGAAAAACCCAGGTCCAAGTCTTTCTCTAATTTGCTTGGTTTAAGGTGGAGAAAGGGGTTTGAAGTTGAAGAGCAAGAAACCAAAGTGAAGCAGCTCAAAAGGAAGTTGGGGTTCAATTTGAGCCATGTACTGAAGAGATATGCGAGAATGGTAAGGCCATTTTTACACTTCAAAGGAAGAAGAGATGATATGCAACTCCGCAGACAGTCTTACTCATTTTCAGGTAACTTAAGTTCGAGAAACAAGCAAGAGTTGAGAGGAAGAAGAGGTCAGTTCTCAGCTCCAGTTTCCATGAGAACATCTCCAACTAATAGTGGTATCCTCCTTACAAAACCACTTCCTCCTTCTACTAATAATGACAGCACAATGGAGGAGTTGCAGGCAGCAATTCAAGCAGCGATTGCTCATTGCAAGAATTCAATATTGAAAGAAGAAAGACTCAAATGT

mRNA sequence

GAAGGCAAAGAAAGGCAATTGGAGAAGCAACGATTGGCAGCTTCTTCATCACCTCCTTCAGCATCCTCATCTCCCTCTCATGAATTTTCTTTCTCAGTTTCTCTGCAAACTTCCTCCACAGACGAAACCAAAGCTCCGCCTTCCTTCACCATTGATTTGTCTCCAGCGGATGACATCTTCTTCCATGGCCATTTGCTTCCTCTCCACCTCCTCTCTCATCTGCCAGTCCCTCCCCGCTGCTCAACCAACTCCATTGAAAGCTATGCCCTTCCCATTAGAGACCTGCTAAGTGAAAAGGAACCCAAGAAAGAGAGCAGCAACTTCATCAATTGCAAGAGCAACGAACACTATCAAAACAGCAACAGTGATGATACAAGAGAAAAACCCAGGTCCAAGTCTTTCTCTAATTTGCTTGGTTTAAGGTGGAGAAAGGGGTTTGAAGTTGAAGAGCAAGAAACCAAAGTGAAGCAGCTCAAAAGGAAGTTGGGGTTCAATTTGAGCCATGTACTGAAGAGATATGCGAGAATGGTAAGGCCATTTTTACACTTCAAAGGAAGAAGAGATGATATGCAACTCCGCAGACAGTCTTACTCATTTTCAGGTAACTTAAGTTCGAGAAACAAGCAAGAGTTGAGAGGAAGAAGAGGTCAGTTCTCAGCTCCAGTTTCCATGAGAACATCTCCAACTAATAGTGGTATCCTCCTTACAAAACCACTTCCTCCTTCTACTAATAATGACAGCACAATGGAGGAGTTGCAGGCAGCAATTCAAGCAGCGATTGCTCATTGCAAGAATTCAATATTGAAAGAAGAAAGACTCAAATGT

Coding sequence (CDS)

GAAGGCAAAGAAAGGCAATTGGAGAAGCAACGATTGGCAGCTTCTTCATCACCTCCTTCAGCATCCTCATCTCCCTCTCATGAATTTTCTTTCTCAGTTTCTCTGCAAACTTCCTCCACAGACGAAACCAAAGCTCCGCCTTCCTTCACCATTGATTTGTCTCCAGCGGATGACATCTTCTTCCATGGCCATTTGCTTCCTCTCCACCTCCTCTCTCATCTGCCAGTCCCTCCCCGCTGCTCAACCAACTCCATTGAAAGCTATGCCCTTCCCATTAGAGACCTGCTAAGTGAAAAGGAACCCAAGAAAGAGAGCAGCAACTTCATCAATTGCAAGAGCAACGAACACTATCAAAACAGCAACAGTGATGATACAAGAGAAAAACCCAGGTCCAAGTCTTTCTCTAATTTGCTTGGTTTAAGGTGGAGAAAGGGGTTTGAAGTTGAAGAGCAAGAAACCAAAGTGAAGCAGCTCAAAAGGAAGTTGGGGTTCAATTTGAGCCATGTACTGAAGAGATATGCGAGAATGGTAAGGCCATTTTTACACTTCAAAGGAAGAAGAGATGATATGCAACTCCGCAGACAGTCTTACTCATTTTCAGGTAACTTAAGTTCGAGAAACAAGCAAGAGTTGAGAGGAAGAAGAGGTCAGTTCTCAGCTCCAGTTTCCATGAGAACATCTCCAACTAATAGTGGTATCCTCCTTACAAAACCACTTCCTCCTTCTACTAATAATGACAGCACAATGGAGGAGTTGCAGGCAGCAATTCAAGCAGCGATTGCTCATTGCAAGAATTCAATATTGAAAGAAGAAAGACTCAAATGT

Protein sequence

EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIFFHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNSNSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPFLHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLPPSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Homology
BLAST of MC08g0613 vs. ExPASy Swiss-Prot
Match: Q9FMZ0 (BRI1 kinase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=BKI1 PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.3e-44
Identity = 145/317 (45.74%), Postives = 188/317 (59.31%), Query Frame = 0

Query: 4   ERQLEKQRLAASSSPPSAS-SSPSHEFSFSVSLQTSSTDETKAPPS-------------- 63
           E+Q  KQ   AS SP S++ SSPSH+FSF++SLQ  S+      P+              
Sbjct: 17  EKQNPKQE--ASPSPISSTCSSPSHDFSFTISLQPLSSSSKHISPTLRSPSKTTSSYQQT 76

Query: 64  --FTIDLSPADDIFFHGHLLPLHLLSHLPVPPRCSTNSI-ESYALPIRDLLSEKEPKKES 123
             F +DLSPAD+IFFHGHLLPLHLLSHLPV PR ST S  + + LP++D+L + +P   +
Sbjct: 77  DPFAVDLSPADEIFFHGHLLPLHLLSHLPVSPRTSTGSYNDGFTLPVKDILPD-QPTNNN 136

Query: 124 SNFINCKSN--EHYQNSNSDDTRE-------KPRSKSFSNLLGL-RWRKGFE--VEEQET 183
           +N  N  +N     ++ N++D  E       KP  KSFS L GL +WRKGFE    EQE 
Sbjct: 137 NNTENAITNISTEAKDDNTEDKAEGEIRVKTKP-IKSFS-LFGLSKWRKGFESNEREQEQ 196

Query: 184 KVKQLKRKLGFNLSHVLKRYARMVRPFLHFKGRRDDMQLRRQ--SYSFSGNL-------- 243
           + +++K+ +  +LSH +K+Y RM+      +G       RRQ  SYSFS +L        
Sbjct: 197 QQQKIKKPMSLDLSHAVKKYIRML---FQKRGNGTQFWNRRQTSSYSFSSSLMGPNGNSK 256

Query: 244 ----SSRNKQEL-RGRRGQ-FSAPVSMRTSPTNSGILLTKPL--------PPSTNNDSTM 267
                S NK++L RGRRG+ FSAP SMRTSPTNSG L               S+++DSTM
Sbjct: 257 TMINGSYNKRDLIRGRRGELFSAPASMRTSPTNSGHLRVSTAGLSSSSGSTSSSSSDSTM 316

BLAST of MC08g0613 vs. ExPASy Swiss-Prot
Match: Q67J19 (Probable BRI1 kinase inhibitor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BKI1 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.5e-14
Identity = 72/184 (39.13%), Postives = 99/184 (53.80%), Query Frame = 0

Query: 105 SSNFINCKSNEHYQNSNSDDTREKPR--------SKSFSNLLGLRWRKGFEVEEQETKVK 164
           S N  + K+N+H Q  +S  ++EK R        S  F + LG  WR G    ++E K K
Sbjct: 78  SHNNYSSKANQHRQTGSSSSSKEKDREYKAKSKASSPFFSGLGGSWRSGLS-RDEEVKRK 137

Query: 165 QLKRKLGFNLSHVLKRY-ARMVRPFL-----HFKGRRDDM-QLRRQSYSFSGNLSS---R 224
              +  G ++   +K+Y A MV   L     H  G R+   Q RR+ +SFS +  S    
Sbjct: 138 AKAKTRGLDVGQWVKKYMASMVEHLLASFSRHGGGEREKREQQRRRPHSFSAHGPSALRE 197

Query: 225 NKQELRGRRGQF-SAPVSMRTSPTNSGILLT--KPLPPSTNNDSTMEELQAAIQAAIAHC 268
            ++  R RRGQ  SAP S+R SP NSG L         +++ +STMEELQ+AI+AAIAHC
Sbjct: 198 QRERWRRRRGQLSSAPASLRASPVNSGHLSVGGSVKVSTSSEESTMEELQSAIEAAIAHC 257

BLAST of MC08g0613 vs. ExPASy Swiss-Prot
Match: A2Z225 (Probable BRI1 kinase inhibitor 1 OS=Oryza sativa subsp. indica OX=39946 GN=BKI1 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.3e-13
Identity = 73/186 (39.25%), Postives = 101/186 (54.30%), Query Frame = 0

Query: 105 SSNFINCKSNEHYQ--NSNSDDTREKPR--------SKSFSNLLGLRWRKGFEVEEQETK 164
           S N  + K+N+H Q  +S+S  ++EK R        S  F + LG  WR G    ++E K
Sbjct: 78  SHNNYSSKANQHRQTGSSSSSSSKEKDREYKAKSKASSPFFSGLGGSWRSGLS-RDEEVK 137

Query: 165 VKQLKRKLGFNLSHVLKRY-ARMVRPFL-----HFKGRRDDM-QLRRQSYSFSGNLSS-- 224
            K   +  G ++   +K+Y A MV   L     H  G R+   Q RR+ +SFS +  S  
Sbjct: 138 RKAKAKTRGLDVGQWVKKYMASMVEHLLASFSRHGGGEREKREQQRRRPHSFSAHGPSAL 197

Query: 225 -RNKQELRGRRGQF-SAPVSMRTSPTNSGILLT--KPLPPSTNNDSTMEELQAAIQAAIA 268
              ++  R RRGQ  SAP S+R SP NSG L         +++ +STMEELQ+AI+AAIA
Sbjct: 198 REQRERWRRRRGQLSSAPASLRASPVNSGHLSVGGSVKVSTSSEESTMEELQSAIEAAIA 257

BLAST of MC08g0613 vs. ExPASy Swiss-Prot
Match: Q3E936 (Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 GN=MAKR1 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.3e-07
Identity = 85/346 (24.57%), Postives = 137/346 (39.60%), Query Frame = 0

Query: 7   LEKQRLAASSSPPSASSSP---SHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIFFHG 66
           + +Q     +SPP + SSP   S EF F++S+         +P   +  L PAD++F+ G
Sbjct: 1   MRRQPPRPRNSPPQSHSSPSSSSSEFEFNISI---------SPRKASSSLCPADELFYKG 60

Query: 67  HLLPLHLLSHLPV----------PPRCSTNSIESYALPIRDLLSEKEPKKESSNF----- 126
            LLPL L   L +              S++S        RD          +++F     
Sbjct: 61  QLLPLQLSPRLSLVRTLGSSTSSSDYTSSSSSSVATSAARDSTESNSSTDSTASFPLLHP 120

Query: 127 --INCKSNEHYQNSNSDDT--REKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLK----- 186
             ++C  +    +   D+    + P++KS S    L        ++ +T +         
Sbjct: 121 PPLDCCDSSRPSSVTDDEDFFFKPPKNKSSSGGFSLSRFSSVFKKDPKTNLHHHSSSSST 180

Query: 187 ----------RKLGFNLSHVLKRYARMVRPFLHFKGRRDDMQLRRQ-------------- 246
                     +++      V+++Y + V+P       +    ++ +              
Sbjct: 181 ATTAAAPSSVKRMSSTAKEVIRKYMKKVKPLYEKLSPKQSSNIKTESSSSLKDSGNNIRG 240

Query: 247 --------------------SYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILL 271
                               S SFSGNL    K   RGR    S P SMR+SP +SG+L 
Sbjct: 241 TTTVTTVTAAPTVVSSGGGLSISFSGNLMKYTK---RGRCAA-SCPSSMRSSPNHSGVLT 300

BLAST of MC08g0613 vs. NCBI nr
Match: XP_022144179.1 (BRI1 kinase inhibitor 1-like [Momordica charantia])

HSP 1 Score: 533 bits (1373), Expect = 2.03e-190
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 60
           EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF
Sbjct: 19  EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 78

Query: 61  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 120
           FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS
Sbjct: 79  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 138

Query: 121 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 180
           NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF
Sbjct: 139 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 198

Query: 181 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP 240
           LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP
Sbjct: 199 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP 258

Query: 241 PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
           PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 259 PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 293

BLAST of MC08g0613 vs. NCBI nr
Match: XP_023545418.1 (BRI1 kinase inhibitor 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448 bits (1152), Expect = 1.22e-156
Identity = 236/275 (85.82%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 60
           EGK+RQLEKQ+L ASSSPPS  SSPSHEFSFSVSLQTSSTD+ KAPPSFTIDLSPADDIF
Sbjct: 30  EGKDRQLEKQQLPASSSPPS--SSPSHEFSFSVSLQTSSTDKAKAPPSFTIDLSPADDIF 89

Query: 61  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 120
           FHGHLLPLHLLSHLPVPPRCSTNSIESY LPIRDLLS+KEPKKESS+FINC S+EHYQN 
Sbjct: 90  FHGHLLPLHLLSHLPVPPRCSTNSIESYTLPIRDLLSDKEPKKESSDFINCNSDEHYQN- 149

Query: 121 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 180
           N  DTREK +SKSFS LLGLRWRKG+E EEQE KVKQLKRK GFN SHVLKRYAR++RPF
Sbjct: 150 NRSDTREKAKSKSFS-LLGLRWRKGYEDEEQENKVKQLKRKFGFNFSHVLKRYARIMRPF 209

Query: 181 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP 240
           LHF+GRRDDMQ  RQSYSFSGNLS R K ELRGRRG+FSAPVSMRTSPTNSG+LLT    
Sbjct: 210 LHFRGRRDDMQPHRQSYSFSGNLSLRKKPELRGRRGEFSAPVSMRTSPTNSGVLLTTTTH 269

Query: 241 PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
           P +NNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 270 PPSNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 300

BLAST of MC08g0613 vs. NCBI nr
Match: XP_022962600.1 (BRI1 kinase inhibitor 1-like [Cucurbita moschata])

HSP 1 Score: 445 bits (1144), Expect = 2.09e-155
Identity = 239/277 (86.28%), Postives = 252/277 (90.97%), Query Frame = 0

Query: 1   EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 60
           EGK+RQLEKQ+L ASSSPPS  SSPSHEFSFSVSLQTSSTD+ KAPPSFTIDLSPADDIF
Sbjct: 30  EGKDRQLEKQQLPASSSPPS--SSPSHEFSFSVSLQTSSTDKAKAPPSFTIDLSPADDIF 89

Query: 61  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 120
           FHGHLLPLHLLSHLPVPPRCSTNSIESY+LPIRDLL +KEPKKESS+FINC S+EHYQN 
Sbjct: 90  FHGHLLPLHLLSHLPVPPRCSTNSIESYSLPIRDLLRDKEPKKESSDFINCNSDEHYQN- 149

Query: 121 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 180
           N  DTREK +SKSFS LLGLRWRKG+E EEQE KVKQLKRK GFN SHVLKRYAR+VRPF
Sbjct: 150 NRSDTREKAKSKSFS-LLGLRWRKGYEDEEQENKVKQLKRKFGFNFSHVLKRYARIVRPF 209

Query: 181 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKP-- 240
           LHF+GRRDDMQ  RQSYSFSGNLS R K ELRGRRG+FSAPVSMRTSPTNSG+LLT    
Sbjct: 210 LHFRGRRDDMQPHRQSYSFSGNLSLRKKPELRGRRGEFSAPVSMRTSPTNSGVLLTTTTT 269

Query: 241 LPPSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
           LPPS NNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 270 LPPS-NNDSTMEELQAAIQAAIAHCKNSILKEERLKC 301

BLAST of MC08g0613 vs. NCBI nr
Match: KAG7029345.1 (BRI1 kinase inhibitor 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 445 bits (1144), Expect = 2.09e-155
Identity = 239/277 (86.28%), Postives = 252/277 (90.97%), Query Frame = 0

Query: 1   EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 60
           EGK+RQLEKQ+L ASSSPPS  SSPSHEFSFSVSLQTSSTD+ KAPPSFTIDLSPADDIF
Sbjct: 30  EGKDRQLEKQQLPASSSPPS--SSPSHEFSFSVSLQTSSTDKAKAPPSFTIDLSPADDIF 89

Query: 61  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 120
           FHGHLLPLHLLSHLPVPPRCSTNSIESY+LPIRDLL +KEPKKESS+FINC S+EHYQN 
Sbjct: 90  FHGHLLPLHLLSHLPVPPRCSTNSIESYSLPIRDLLRDKEPKKESSDFINCNSDEHYQN- 149

Query: 121 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 180
           N  DTREK +SKSFS LLGLRWRKG+E EEQE KVKQLKRK GFN SHVLKRYAR+VRPF
Sbjct: 150 NRSDTREKAKSKSFS-LLGLRWRKGYEDEEQENKVKQLKRKFGFNFSHVLKRYARIVRPF 209

Query: 181 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKP-- 240
           LHF+GRRDDMQ  RQSYSFSGNLS R K ELRGRRG+FSAPVSMRTSPTNSG+LLT    
Sbjct: 210 LHFRGRRDDMQPHRQSYSFSGNLSLRKKPELRGRRGEFSAPVSMRTSPTNSGVLLTTTTT 269

Query: 241 LPPSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
           LPPS NNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 270 LPPS-NNDSTMEELQAAIQAAIAHCKNSILKEERLKC 301

BLAST of MC08g0613 vs. NCBI nr
Match: XP_022997305.1 (BRI1 kinase inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 444 bits (1141), Expect = 5.78e-155
Identity = 234/275 (85.09%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 1   EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 60
           EG+ERQLEKQ+L ASSSPP   SSPSHEFSFSVSLQTSS+D+ KAPPSFTIDLSPADDIF
Sbjct: 30  EGEERQLEKQQLPASSSPPP--SSPSHEFSFSVSLQTSSSDKAKAPPSFTIDLSPADDIF 89

Query: 61  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 120
           FHGHLLPLHLLSHLPVPPRCSTNSIESY LPIRDLL++KEPKKESS+FINC S+EHYQN 
Sbjct: 90  FHGHLLPLHLLSHLPVPPRCSTNSIESYTLPIRDLLNDKEPKKESSDFINCNSDEHYQN- 149

Query: 121 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 180
           N  DTREK +SKSFS LLGLRWRKG+E EEQE KVKQLKRK GFN SHVLKRYAR+VRPF
Sbjct: 150 NRSDTREKAKSKSFS-LLGLRWRKGYEDEEQENKVKQLKRKFGFNFSHVLKRYARIVRPF 209

Query: 181 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP 240
           LHF+GRRDDMQ  RQSYSFSGNLS R K ELRGRRG+FSAPVSMRTSPTNSG+LLT    
Sbjct: 210 LHFRGRRDDMQPHRQSYSFSGNLSLRKKPELRGRRGEFSAPVSMRTSPTNSGVLLTTTTL 269

Query: 241 PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
           P +NNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 270 PPSNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 300

BLAST of MC08g0613 vs. ExPASy TrEMBL
Match: A0A6J1CRJ3 (BRI1 kinase inhibitor 1-like OS=Momordica charantia OX=3673 GN=LOC111013931 PE=4 SV=1)

HSP 1 Score: 533 bits (1373), Expect = 9.83e-191
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 60
           EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF
Sbjct: 19  EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 78

Query: 61  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 120
           FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS
Sbjct: 79  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 138

Query: 121 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 180
           NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF
Sbjct: 139 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 198

Query: 181 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP 240
           LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP
Sbjct: 199 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP 258

Query: 241 PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
           PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 259 PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 293

BLAST of MC08g0613 vs. ExPASy TrEMBL
Match: A0A6J1HFJ7 (BRI1 kinase inhibitor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111463002 PE=4 SV=1)

HSP 1 Score: 445 bits (1144), Expect = 1.01e-155
Identity = 239/277 (86.28%), Postives = 252/277 (90.97%), Query Frame = 0

Query: 1   EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 60
           EGK+RQLEKQ+L ASSSPPS  SSPSHEFSFSVSLQTSSTD+ KAPPSFTIDLSPADDIF
Sbjct: 30  EGKDRQLEKQQLPASSSPPS--SSPSHEFSFSVSLQTSSTDKAKAPPSFTIDLSPADDIF 89

Query: 61  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 120
           FHGHLLPLHLLSHLPVPPRCSTNSIESY+LPIRDLL +KEPKKESS+FINC S+EHYQN 
Sbjct: 90  FHGHLLPLHLLSHLPVPPRCSTNSIESYSLPIRDLLRDKEPKKESSDFINCNSDEHYQN- 149

Query: 121 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 180
           N  DTREK +SKSFS LLGLRWRKG+E EEQE KVKQLKRK GFN SHVLKRYAR+VRPF
Sbjct: 150 NRSDTREKAKSKSFS-LLGLRWRKGYEDEEQENKVKQLKRKFGFNFSHVLKRYARIVRPF 209

Query: 181 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKP-- 240
           LHF+GRRDDMQ  RQSYSFSGNLS R K ELRGRRG+FSAPVSMRTSPTNSG+LLT    
Sbjct: 210 LHFRGRRDDMQPHRQSYSFSGNLSLRKKPELRGRRGEFSAPVSMRTSPTNSGVLLTTTTT 269

Query: 241 LPPSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
           LPPS NNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 270 LPPS-NNDSTMEELQAAIQAAIAHCKNSILKEERLKC 301

BLAST of MC08g0613 vs. ExPASy TrEMBL
Match: A0A6J1K982 (BRI1 kinase inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492254 PE=4 SV=1)

HSP 1 Score: 444 bits (1141), Expect = 2.80e-155
Identity = 234/275 (85.09%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 1   EGKERQLEKQRLAASSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIF 60
           EG+ERQLEKQ+L ASSSPP   SSPSHEFSFSVSLQTSS+D+ KAPPSFTIDLSPADDIF
Sbjct: 30  EGEERQLEKQQLPASSSPPP--SSPSHEFSFSVSLQTSSSDKAKAPPSFTIDLSPADDIF 89

Query: 61  FHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQNS 120
           FHGHLLPLHLLSHLPVPPRCSTNSIESY LPIRDLL++KEPKKESS+FINC S+EHYQN 
Sbjct: 90  FHGHLLPLHLLSHLPVPPRCSTNSIESYTLPIRDLLNDKEPKKESSDFINCNSDEHYQN- 149

Query: 121 NSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRPF 180
           N  DTREK +SKSFS LLGLRWRKG+E EEQE KVKQLKRK GFN SHVLKRYAR+VRPF
Sbjct: 150 NRSDTREKAKSKSFS-LLGLRWRKGYEDEEQENKVKQLKRKFGFNFSHVLKRYARIVRPF 209

Query: 181 LHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILLTKPLP 240
           LHF+GRRDDMQ  RQSYSFSGNLS R K ELRGRRG+FSAPVSMRTSPTNSG+LLT    
Sbjct: 210 LHFRGRRDDMQPHRQSYSFSGNLSLRKKPELRGRRGEFSAPVSMRTSPTNSGVLLTTTTL 269

Query: 241 PSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
           P +NNDSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 270 PPSNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 300

BLAST of MC08g0613 vs. ExPASy TrEMBL
Match: A0A0A0LLA3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359970 PE=4 SV=1)

HSP 1 Score: 431 bits (1109), Expect = 1.13e-150
Identity = 236/278 (84.89%), Postives = 252/278 (90.65%), Query Frame = 0

Query: 1   EGKERQLEKQRLAA-SSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDI 60
           E KERQLEKQ+L A SSSP SASSSPSHEFSFSVSLQTSSTD+ K  PSFTIDLSPADDI
Sbjct: 8   EDKERQLEKQQLPANSSSPHSASSSPSHEFSFSVSLQTSSTDKAKTSPSFTIDLSPADDI 67

Query: 61  FFHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQN 120
           FFHGHLLPLHLLSHLPVPPRCSTNS+ESY LPIRDL+SEKEPKKESS+FINCKSNEH QN
Sbjct: 68  FFHGHLLPLHLLSHLPVPPRCSTNSMESYTLPIRDLISEKEPKKESSSFINCKSNEHCQN 127

Query: 121 SNSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRP 180
            N++DTREKP+SKSFS LLGLRWRK  +VEEQE KV+Q KRKLGFNLS+VLKRYARMVRP
Sbjct: 128 -NTNDTREKPKSKSFS-LLGLRWRKRSDVEEQENKVEQEKRKLGFNLSNVLKRYARMVRP 187

Query: 181 FLHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQF-SAPVSMRTSPTNSGILLTKP 240
           FL  +GRRDDMQL RQSYSFSGNL  R K+E RGRRG+F SAPVSMRTSPTNSG+LLT  
Sbjct: 188 FLPLRGRRDDMQLHRQSYSFSGNLRLRKKRESRGRRGEFFSAPVSMRTSPTNSGVLLTTT 247

Query: 241 -LPPSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
            LPPSTN+DSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 248 TLPPSTNSDSTMEELQAAIQAAIAHCKNSILKEERLKC 283

BLAST of MC08g0613 vs. ExPASy TrEMBL
Match: A0A5A7UYB0 (BRI1 kinase inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00140 PE=4 SV=1)

HSP 1 Score: 413 bits (1061), Expect = 2.07e-143
Identity = 230/278 (82.73%), Postives = 246/278 (88.49%), Query Frame = 0

Query: 1   EGKERQLEKQRLAA-SSSPPSASSSPSHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDI 60
           E KERQLEKQ+L A SSSP SASSSPSHEFSFSVSLQTS TD+ K  PSFTIDLSPADDI
Sbjct: 8   EDKERQLEKQQLPANSSSPHSASSSPSHEFSFSVSLQTSFTDKAKTSPSFTIDLSPADDI 67

Query: 61  FFHGHLLPLHLLSHLPVPPRCSTNSIESYALPIRDLLSEKEPKKESSNFINCKSNEHYQN 120
           FFHGHLLPLHLLSHLPVPPRCSTNS+ESY LPIRDL+SEKEPKKESS+FIN K NEH QN
Sbjct: 68  FFHGHLLPLHLLSHLPVPPRCSTNSMESYTLPIRDLISEKEPKKESSSFINRKRNEHCQN 127

Query: 121 SNSDDTREKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLKRKLGFNLSHVLKRYARMVRP 180
            NS+DTREKP+SKSFS LLGLRWRK  +VE+QE KV+Q KRKLGFNLS+VLKRYARMV P
Sbjct: 128 -NSNDTREKPKSKSFS-LLGLRWRKRSDVEDQENKVEQDKRKLGFNLSNVLKRYARMVTP 187

Query: 181 FLHFKGRRDDMQLRRQSYSFSGNLSSRNKQELRGRRGQF-SAPVSMRTSPTNSGILLTKP 240
           FLH +G+RDDMQL RQ  SFSGNL  R K   RGRRG+F SAPVSMRTSPTNSG+LLT P
Sbjct: 188 FLHLRGKRDDMQLHRQYNSFSGNLRLRKK---RGRRGEFFSAPVSMRTSPTNSGVLLTTP 247

Query: 241 -LPPSTNNDSTMEELQAAIQAAIAHCKNSILKEERLKC 275
            LPPSTN+DSTMEELQAAIQAAIAHCKNSILKEERLKC
Sbjct: 248 TLPPSTNSDSTMEELQAAIQAAIAHCKNSILKEERLKC 280

BLAST of MC08g0613 vs. TAIR 10
Match: AT5G42750.1 (BRI1 kinase inhibitor 1 )

HSP 1 Score: 180.6 bits (457), Expect = 1.7e-45
Identity = 145/317 (45.74%), Postives = 188/317 (59.31%), Query Frame = 0

Query: 4   ERQLEKQRLAASSSPPSAS-SSPSHEFSFSVSLQTSSTDETKAPPS-------------- 63
           E+Q  KQ   AS SP S++ SSPSH+FSF++SLQ  S+      P+              
Sbjct: 17  EKQNPKQE--ASPSPISSTCSSPSHDFSFTISLQPLSSSSKHISPTLRSPSKTTSSYQQT 76

Query: 64  --FTIDLSPADDIFFHGHLLPLHLLSHLPVPPRCSTNSI-ESYALPIRDLLSEKEPKKES 123
             F +DLSPAD+IFFHGHLLPLHLLSHLPV PR ST S  + + LP++D+L + +P   +
Sbjct: 77  DPFAVDLSPADEIFFHGHLLPLHLLSHLPVSPRTSTGSYNDGFTLPVKDILPD-QPTNNN 136

Query: 124 SNFINCKSN--EHYQNSNSDDTRE-------KPRSKSFSNLLGL-RWRKGFE--VEEQET 183
           +N  N  +N     ++ N++D  E       KP  KSFS L GL +WRKGFE    EQE 
Sbjct: 137 NNTENAITNISTEAKDDNTEDKAEGEIRVKTKP-IKSFS-LFGLSKWRKGFESNEREQEQ 196

Query: 184 KVKQLKRKLGFNLSHVLKRYARMVRPFLHFKGRRDDMQLRRQ--SYSFSGNL-------- 243
           + +++K+ +  +LSH +K+Y RM+      +G       RRQ  SYSFS +L        
Sbjct: 197 QQQKIKKPMSLDLSHAVKKYIRML---FQKRGNGTQFWNRRQTSSYSFSSSLMGPNGNSK 256

Query: 244 ----SSRNKQEL-RGRRGQ-FSAPVSMRTSPTNSGILLTKPL--------PPSTNNDSTM 267
                S NK++L RGRRG+ FSAP SMRTSPTNSG L               S+++DSTM
Sbjct: 257 TMINGSYNKRDLIRGRRGELFSAPASMRTSPTNSGHLRVSTAGLSSSSGSTSSSSSDSTM 316

BLAST of MC08g0613 vs. TAIR 10
Match: AT5G26230.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 57.8 bits (138), Expect = 1.6e-08
Identity = 85/346 (24.57%), Postives = 137/346 (39.60%), Query Frame = 0

Query: 7   LEKQRLAASSSPPSASSSP---SHEFSFSVSLQTSSTDETKAPPSFTIDLSPADDIFFHG 66
           + +Q     +SPP + SSP   S EF F++S+         +P   +  L PAD++F+ G
Sbjct: 1   MRRQPPRPRNSPPQSHSSPSSSSSEFEFNISI---------SPRKASSSLCPADELFYKG 60

Query: 67  HLLPLHLLSHLPV----------PPRCSTNSIESYALPIRDLLSEKEPKKESSNF----- 126
            LLPL L   L +              S++S        RD          +++F     
Sbjct: 61  QLLPLQLSPRLSLVRTLGSSTSSSDYTSSSSSSVATSAARDSTESNSSTDSTASFPLLHP 120

Query: 127 --INCKSNEHYQNSNSDDT--REKPRSKSFSNLLGLRWRKGFEVEEQETKVKQLK----- 186
             ++C  +    +   D+    + P++KS S    L        ++ +T +         
Sbjct: 121 PPLDCCDSSRPSSVTDDEDFFFKPPKNKSSSGGFSLSRFSSVFKKDPKTNLHHHSSSSST 180

Query: 187 ----------RKLGFNLSHVLKRYARMVRPFLHFKGRRDDMQLRRQ-------------- 246
                     +++      V+++Y + V+P       +    ++ +              
Sbjct: 181 ATTAAAPSSVKRMSSTAKEVIRKYMKKVKPLYEKLSPKQSSNIKTESSSSLKDSGNNIRG 240

Query: 247 --------------------SYSFSGNLSSRNKQELRGRRGQFSAPVSMRTSPTNSGILL 271
                               S SFSGNL    K   RGR    S P SMR+SP +SG+L 
Sbjct: 241 TTTVTTVTAAPTVVSSGGGLSISFSGNLMKYTK---RGRCAA-SCPSSMRSSPNHSGVLT 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMZ02.3e-4445.74BRI1 kinase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=BKI1 PE=1 SV=1[more]
Q67J191.5e-1439.13Probable BRI1 kinase inhibitor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BKI... [more]
A2Z2251.3e-1339.25Probable BRI1 kinase inhibitor 1 OS=Oryza sativa subsp. indica OX=39946 GN=BKI1 ... [more]
Q3E9362.3e-0724.57Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_022144179.12.03e-190100.00BRI1 kinase inhibitor 1-like [Momordica charantia][more]
XP_023545418.11.22e-15685.82BRI1 kinase inhibitor 1-like [Cucurbita pepo subsp. pepo][more]
XP_022962600.12.09e-15586.28BRI1 kinase inhibitor 1-like [Cucurbita moschata][more]
KAG7029345.12.09e-15586.28BRI1 kinase inhibitor 1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022997305.15.78e-15585.09BRI1 kinase inhibitor 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CRJ39.83e-191100.00BRI1 kinase inhibitor 1-like OS=Momordica charantia OX=3673 GN=LOC111013931 PE=4... [more]
A0A6J1HFJ71.01e-15586.28BRI1 kinase inhibitor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111463002 PE=4 ... [more]
A0A6J1K9822.80e-15585.09BRI1 kinase inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492254 PE=4 SV... [more]
A0A0A0LLA31.13e-15084.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359970 PE=4 SV=1[more]
A0A5A7UYB02.07e-14382.73BRI1 kinase inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
Match NameE-valueIdentityDescription
AT5G42750.11.7e-4545.74BRI1 kinase inhibitor 1 [more]
AT5G26230.11.6e-0824.57unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..49
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 220..247
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 203..247
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..131
NoneNo IPR availablePANTHERPTHR33312:SF19BRI1 KINASE INHIBITOR 1coord: 6..274
IPR039620BKI1/Probable membrane-associated kinase regulator 1/3/4PANTHERPTHR33312MEMBRANE-ASSOCIATED KINASE REGULATOR 4-RELATEDcoord: 6..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0613.1MC08g0613.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
cellular_component GO:0005886 plasma membrane
molecular_function GO:0019210 kinase inhibitor activity