MC08g0610 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0610
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	calmodulin-like
Location	MC08: 4996653 .. 4997054 (+)
RNA-Seq Expression	MC08g0610
Synteny	MC08g0610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCATGTGAATGAAGCTGAAGCTAGTGAAATGATCCAAAAGTATGACAAAAATGGCGACTCTATCATCACCAAGGAAGAGATCGTCGACAAAGCTGTCAATCATAAGGCTAATGTCGTACCAAAAGCTCAAAAAGTCCCCGCTAGAGTGGCGAATGAAAAGAAGGCACCGAAGATAAAGTTCAGTCGGAGCCAAATCAAGGAGATTTTCATGGAGCACGATATCGATGGTGATGGTTGCCTTAGTGTAAGTGAGCTGATAAAGGCTTTTAGTTTTCTAGGTTCAGTCATTCCACTTTACAAAGCTCACTACGGTCTAGCTTTTGCTGATGATGATGGAGATGGCCTTATCAGCGAGGAAGAACTTGACAAACTCGTTGATTACGCCGAGAAAGTATGTAAT

mRNA sequence

TCTCATGTGAATGAAGCTGAAGCTAGTGAAATGATCCAAAAGTATGACAAAAATGGCGACTCTATCATCACCAAGGAAGAGATCGTCGACAAAGCTGTCAATCATAAGGCTAATGTCGTACCAAAAGCTCAAAAAGTCCCCGCTAGAGTGGCGAATGAAAAGAAGGCACCGAAGATAAAGTTCAGTCGGAGCCAAATCAAGGAGATTTTCATGGAGCACGATATCGATGGTGATGGTTGCCTTAGTGTAAGTGAGCTGATAAAGGCTTTTAGTTTTCTAGGTTCAGTCATTCCACTTTACAAAGCTCACTACGGTCTAGCTTTTGCTGATGATGATGGAGATGGCCTTATCAGCGAGGAAGAACTTGACAAACTCGTTGATTACGCCGAGAAAGTATGTAAT

Coding sequence (CDS)

TCTCATGTGAATGAAGCTGAAGCTAGTGAAATGATCCAAAAGTATGACAAAAATGGCGACTCTATCATCACCAAGGAAGAGATCGTCGACAAAGCTGTCAATCATAAGGCTAATGTCGTACCAAAAGCTCAAAAAGTCCCCGCTAGAGTGGCGAATGAAAAGAAGGCACCGAAGATAAAGTTCAGTCGGAGCCAAATCAAGGAGATTTTCATGGAGCACGATATCGATGGTGATGGTTGCCTTAGTGTAAGTGAGCTGATAAAGGCTTTTAGTTTTCTAGGTTCAGTCATTCCACTTTACAAAGCTCACTACGGTCTAGCTTTTGCTGATGATGATGGAGATGGCCTTATCAGCGAGGAAGAACTTGACAAACTCGTTGATTACGCCGAGAAAGTATGTAAT

Protein sequence

SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNHKANVVPKAQKVPARVANEKKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLISEEELDKLVDYAEKVCN

Homology

[Close]

BLAST of MC08g0610 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.4e-04
Identity = 36/141 (25.53%), Postives = 66/141 (46.81%), Query Frame = 0


Query: 7   EASEMIQKYDKNGDSIITKEEI---------------VDKAVNH---KANVVPKAQKVPA 66
           E  E+ + +D+NGD  +T+ E+               +D  +      +N + +  +  A
Sbjct: 64  ELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGLIEFSEFVA 123

Query: 67  RVANEKKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAF 126
            VA E    +  +S  QI+ +F   D DG+G ++ +EL  + + LG  + + +    +  
Sbjct: 124 LVAPELLYDRAPYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKLGHALTVKELTGMIKE 183

Query: 127 ADDDGDGLISEEELDKLVDYA 130
           AD DGDG IS +E  + +  A
Sbjct: 184 ADTDGDGRISFQEFSRAITAA 204

[Close]

BLAST of MC08g0610 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 7.5e-04
Identity = 26/67 (38.81%), Postives = 38/67 (56.72%), Query Frame = 0


Query: 65  QIKEIFMEHDIDGDGCLSVSEL-IKAFSFLGSVIPLYKAHYGLAFADDDGDGLISEEELD 124
           + + +F   D DGDG +S +EL +   + LG  +P  +    +A AD DGDGL+ EEE  
Sbjct: 6   EFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFV 65

Query: 125 KLVDYAE 131
           +LV   E
Sbjct: 66  RLVTEME 72

[Close]

BLAST of MC08g0610 vs. NCBI nr
Match: XP_022144156.1 (probable calcium-binding protein CML15 [Momordica charantia])

HSP 1 Score: 254 bits (649), Expect = 1.34e-83
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNHKANVVPKAQKVPARVANEKKAPKIK 60
           SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNHKANVVPKAQKVPARVANEKKAPKIK
Sbjct: 13  SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNHKANVVPKAQKVPARVANEKKAPKIK 72

Query: 61  FSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLISEE 120
           FSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLISEE
Sbjct: 73  FSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLISEE 132

Query: 121 ELDKLVDYAEK 131
           ELDKLVDYAEK
Sbjct: 133 ELDKLVDYAEK 143

[Close]

BLAST of MC08g0610 vs. NCBI nr
Match: XP_023002888.1 (calmodulin-like [Cucurbita maxima])

HSP 1 Score: 171 bits (432), Expect = 1.61e-51
Identity = 96/145 (66.21%), Postives = 109/145 (75.17%), Query Frame = 0


Query: 1   SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNH--------------KANVVPKAQKV 60
           +H  EAEA E IQ YDKN DS ++KEE+   A++H              K +V PKAQKV
Sbjct: 13  AHGAEAEAQEFIQNYDKNADSSLSKEEL-SAAIHHNQAKNPGSHKPEAKKKDVAPKAQKV 72

Query: 61  PARVANEKKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGL 120
           PA+ A EK A  +K SRSQIKEIFMEHDIDGDG LSVSEL KAFSF GS+IPLYKAHYGL
Sbjct: 73  PAKAAEEKGAS-MKLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGL 132

Query: 121 AFADDDGDGLISEEELDKLVDYAEK 131
           A+AD DGDGLISEEEL+KLVDYAE+
Sbjct: 133 AYADADGDGLISEEELEKLVDYAER 155

[Close]

BLAST of MC08g0610 vs. NCBI nr
Match: XP_022951879.1 (calmodulin-like [Cucurbita moschata] >XP_023537873.1 calmodulin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 170 bits (431), Expect = 2.29e-51
Identity = 95/145 (65.52%), Postives = 110/145 (75.86%), Query Frame = 0


Query: 1   SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNH--------------KANVVPKAQKV 60
           +H  EAEA E++Q YDKN DS ++KEE+   A++H              K +V PKAQKV
Sbjct: 13  AHGAEAEAQELMQNYDKNADSSLSKEEL-SAAIHHNQAKNPGSHNPEAKKKDVAPKAQKV 72

Query: 61  PARVANEKKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGL 120
           PA+ A EK A  +K SRSQIKEIFMEHDIDGDG LSVSEL KAFSF GS+IPLYKAHYGL
Sbjct: 73  PAKAAEEKGAS-MKLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGL 132

Query: 121 AFADDDGDGLISEEELDKLVDYAEK 131
           A+AD DGDGLISEEEL+KLVDYAE+
Sbjct: 133 AYADADGDGLISEEELEKLVDYAER 155

[Close]

BLAST of MC08g0610 vs. NCBI nr
Match: XP_023002889.1 (calmodulin-A-like [Cucurbita maxima])

HSP 1 Score: 152 bits (384), Expect = 2.64e-44
Identity = 81/138 (58.70%), Postives = 107/138 (77.54%), Query Frame = 0


Query: 2   HVNEAEASEMIQKYDKNGDSIITKEEIV-------DKAVNHKANVVPKAQKVPARVANEK 61
           +++EA+A+ +I+KYDKNGDSI+TK+E+         +   H  +VV K +K PA+V  E 
Sbjct: 14  YMSEADANNLIKKYDKNGDSILTKDELTAMILEQASQQQGHPKDVVCKPKKNPAKVP-ED 73

Query: 62  KAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGD 121
           K   ++ SRS+I++IF+EHDIDGDG L+VSEL KAFSF+GS++PLYKAHYGL +AD DGD
Sbjct: 74  KGLHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKAFSFIGSMLPLYKAHYGLNYADADGD 133

Query: 122 GLISEEELDKLVDYAEKV 132
           G ISEEEL KLVDYAE+V
Sbjct: 134 GRISEEELGKLVDYAERV 150

[Close]

BLAST of MC08g0610 vs. NCBI nr
Match: XP_022951880.1 (calmodulin-like [Cucurbita moschata])

HSP 1 Score: 151 bits (381), Expect = 7.53e-44
Identity = 81/138 (58.70%), Postives = 111/138 (80.43%), Query Frame = 0


Query: 2   HVNEAEASEMIQKYDKNGDSIITKEE----IVDKAVNHKA---NVVPKAQKVPARVANEK 61
           +++EA+A+ +I+KYDKNGDSI+TK+E    I+++A   +    ++V K +K PA+V  E 
Sbjct: 14  YMSEADANNLIKKYDKNGDSILTKDELTAMILEQASQQQGPPKDIVCKPKKKPAKVP-ED 73

Query: 62  KAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGD 121
           K   ++ SRS+I++IF+EHDIDGDG L+VSEL KAFSF+GS++PLYKAHYGL +AD DGD
Sbjct: 74  KGLHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKAFSFIGSMLPLYKAHYGLNYADADGD 133

Query: 122 GLISEEELDKLVDYAEKV 132
           G ISEEEL+KLVDYAE+V
Sbjct: 134 GRISEEELEKLVDYAERV 150

[Close]

BLAST of MC08g0610 vs. ExPASy TrEMBL
Match: A0A6J1CSH1 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111013915 PE=4 SV=1)

HSP 1 Score: 254 bits (649), Expect = 6.49e-84
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNHKANVVPKAQKVPARVANEKKAPKIK 60
           SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNHKANVVPKAQKVPARVANEKKAPKIK
Sbjct: 13  SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNHKANVVPKAQKVPARVANEKKAPKIK 72

Query: 61  FSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLISEE 120
           FSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLISEE
Sbjct: 73  FSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLISEE 132

Query: 121 ELDKLVDYAEK 131
           ELDKLVDYAEK
Sbjct: 133 ELDKLVDYAEK 143

[Close]

BLAST of MC08g0610 vs. ExPASy TrEMBL
Match: A0A6J1KUW2 (calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1)

HSP 1 Score: 171 bits (432), Expect = 7.81e-52
Identity = 96/145 (66.21%), Postives = 109/145 (75.17%), Query Frame = 0


Query: 1   SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNH--------------KANVVPKAQKV 60
           +H  EAEA E IQ YDKN DS ++KEE+   A++H              K +V PKAQKV
Sbjct: 13  AHGAEAEAQEFIQNYDKNADSSLSKEEL-SAAIHHNQAKNPGSHKPEAKKKDVAPKAQKV 72

Query: 61  PARVANEKKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGL 120
           PA+ A EK A  +K SRSQIKEIFMEHDIDGDG LSVSEL KAFSF GS+IPLYKAHYGL
Sbjct: 73  PAKAAEEKGAS-MKLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGL 132

Query: 121 AFADDDGDGLISEEELDKLVDYAEK 131
           A+AD DGDGLISEEEL+KLVDYAE+
Sbjct: 133 AYADADGDGLISEEELEKLVDYAER 155

[Close]

BLAST of MC08g0610 vs. ExPASy TrEMBL
Match: A0A6J1GK16 (calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454616 PE=4 SV=1)

HSP 1 Score: 170 bits (431), Expect = 1.11e-51
Identity = 95/145 (65.52%), Postives = 110/145 (75.86%), Query Frame = 0


Query: 1   SHVNEAEASEMIQKYDKNGDSIITKEEIVDKAVNH--------------KANVVPKAQKV 60
           +H  EAEA E++Q YDKN DS ++KEE+   A++H              K +V PKAQKV
Sbjct: 13  AHGAEAEAQELMQNYDKNADSSLSKEEL-SAAIHHNQAKNPGSHNPEAKKKDVAPKAQKV 72

Query: 61  PARVANEKKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGL 120
           PA+ A EK A  +K SRSQIKEIFMEHDIDGDG LSVSEL KAFSF GS+IPLYKAHYGL
Sbjct: 73  PAKAAEEKGAS-MKLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGL 132

Query: 121 AFADDDGDGLISEEELDKLVDYAEK 131
           A+AD DGDGLISEEEL+KLVDYAE+
Sbjct: 133 AYADADGDGLISEEELEKLVDYAER 155

[Close]

BLAST of MC08g0610 vs. ExPASy TrEMBL
Match: A0A6J1KRR4 (calmodulin-A-like OS=Cucurbita maxima OX=3661 GN=LOC111496627 PE=4 SV=1)

HSP 1 Score: 152 bits (384), Expect = 1.28e-44
Identity = 81/138 (58.70%), Postives = 107/138 (77.54%), Query Frame = 0


Query: 2   HVNEAEASEMIQKYDKNGDSIITKEEIV-------DKAVNHKANVVPKAQKVPARVANEK 61
           +++EA+A+ +I+KYDKNGDSI+TK+E+         +   H  +VV K +K PA+V  E 
Sbjct: 14  YMSEADANNLIKKYDKNGDSILTKDELTAMILEQASQQQGHPKDVVCKPKKNPAKVP-ED 73

Query: 62  KAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGD 121
           K   ++ SRS+I++IF+EHDIDGDG L+VSEL KAFSF+GS++PLYKAHYGL +AD DGD
Sbjct: 74  KGLHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKAFSFIGSMLPLYKAHYGLNYADADGD 133

Query: 122 GLISEEELDKLVDYAEKV 132
           G ISEEEL KLVDYAE+V
Sbjct: 134 GRISEEELGKLVDYAERV 150

[Close]

BLAST of MC08g0610 vs. ExPASy TrEMBL
Match: A0A6J1GK54 (calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454617 PE=4 SV=1)

HSP 1 Score: 151 bits (381), Expect = 3.64e-44
Identity = 81/138 (58.70%), Postives = 111/138 (80.43%), Query Frame = 0


Query: 2   HVNEAEASEMIQKYDKNGDSIITKEE----IVDKAVNHKA---NVVPKAQKVPARVANEK 61
           +++EA+A+ +I+KYDKNGDSI+TK+E    I+++A   +    ++V K +K PA+V  E 
Sbjct: 14  YMSEADANNLIKKYDKNGDSILTKDELTAMILEQASQQQGPPKDIVCKPKKKPAKVP-ED 73

Query: 62  KAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGD 121
           K   ++ SRS+I++IF+EHDIDGDG L+VSEL KAFSF+GS++PLYKAHYGL +AD DGD
Sbjct: 74  KGLHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKAFSFIGSMLPLYKAHYGLNYADADGD 133

Query: 122 GLISEEELDKLVDYAEKV 132
           G ISEEEL+KLVDYAE+V
Sbjct: 134 GRISEEELEKLVDYAERV 150

The following BLAST results are available for this feature:

BLAST of MC08g0610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9AWK2	3.4e-04	25.53	Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYJ9	7.5e-04	38.81	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of MC08g0610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144156.1	1.34e-83	100.00	probable calcium-binding protein CML15 [Momordica charantia]	[more]
XP_023002888.1	1.61e-51	66.21	calmodulin-like [Cucurbita maxima]	[more]
XP_022951879.1	2.29e-51	65.52	calmodulin-like [Cucurbita moschata] >XP_023537873.1 calmodulin-like [Cucurbita ...	[more]
XP_023002889.1	2.64e-44	58.70	calmodulin-A-like [Cucurbita maxima]	[more]
XP_022951880.1	7.53e-44	58.70	calmodulin-like [Cucurbita moschata]	[more]

BLAST of MC08g0610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CSH1	6.49e-84	100.00	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1KUW2	7.81e-52	66.21	calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1	[more]
A0A6J1GK16	1.11e-51	65.52	calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454616 PE=4 SV=1	[more]
A0A6J1KRR4	1.28e-44	58.70	calmodulin-A-like OS=Cucurbita maxima OX=3661 GN=LOC111496627 PE=4 SV=1	[more]
A0A6J1GK54	3.64e-44	58.70	calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454617 PE=4 SV=1	[more]

BLAST of MC08g0610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 7..35 e-value: 1.4 score: 16.5 coord: 65..93 e-value: 0.04 score: 23.0
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 109..126 e-value: 0.0031 score: 17.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 13..90 e-value: 8.2E-8 score: 32.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 3..38 score: 10.747526
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 61..96 score: 13.286007
None	No IPR available	GENE3D	1.10.238.10		coord: 7..130 e-value: 6.4E-15 score: 57.5
None	No IPR available	PANTHER	PTHR10891:SF911	SUBFAMILY NOT NAMED	coord: 55..131
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 55..131
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 74..86
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 110..122
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 3..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0610.1	MC08g0610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding