MC08g0502 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g0502 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 632.1 bits (1629), Expect = 6.2e-180
Identity = 305/578 (52.77%), Postives = 399/578 (69.03%), Query Frame = 0

Query: 11  MGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWM---NGPMYY 70
           M K +   + L  LLS  +   ++  + +      Q  RT YHFQP KNWM   NGPM Y
Sbjct: 1   MAKLNRSNIGLSLLLSMFLANFITDLEASSHQDLNQPYRTGYHFQPLKNWMNDPNGPMIY 60

Query: 71  KGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGSASFL 130
           KG+YHLF+QYNP GAV++ ++VW HS S+DL+NW+   PA  P+ P DINGCWSGS + L
Sbjct: 61  KGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTIL 120

Query: 131 PGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPII--SPPDHVLGDRFRD 190
           P  KPVILYTGID +  QVQN+A+P N SDP+L +W+K P NP++  +  + +  DRFRD
Sbjct: 121 PNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRD 180

Query: 191 PTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPDFF 250
           PTTAW G DG WRV +G  T D  G A+LY+S DF  W     PL+    +G WECPDFF
Sbjct: 181 PTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFF 240

Query: 251 PVLLNGTNGVDFSSGFGIGVKHVMKASF--NSHDYYTLGSYAPEEEKFTPDSGSDFGFRG 310
           PV + G++GV+ SS    G+KHV+K S     HDYYT+GSY  E++ + P    D GF  
Sbjct: 241 PVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVP----DLGFVQ 300

Query: 311 TNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSK 370
                R DYGK+YASKTFYD  +KRRILWGWVNES   +DDI+KGWSGLQ+ PRKIWL +
Sbjct: 301 NESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDE 360

Query: 371 TGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAER 430
           +G++L+QWP+EEI+ LR    +   K L   ST++V G +A+Q D EVSF+   LE+A+ 
Sbjct: 361 SGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADV 420

Query: 431 MDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAH------KRYV 490
           ++  W DPQ +CS+ + SV   +GPFGL+VLASND+ E T+V+F I K++       +YV
Sbjct: 421 IEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYV 480

Query: 491 VLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRV 550
           VLMCSDQSRSS  +  DK+T+G+FV IDP H+ ISLRTL+DHS+VES+ G G+TCITSRV
Sbjct: 481 VLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRV 540

Query: 551 YPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQIN 576
           YP LA+ ++A L+ FN GTQSV I +L+ W++  AQIN
Sbjct: 541 YPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of MC08g0502 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 3.6e-172
Identity = 303/544 (55.70%), Postives = 386/544 (70.96%), Query Frame = 0

Query: 46  QALRTSYHFQPPKNWM---NGPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWV 105
           Q  RT +HFQPPKNWM   NGPM YKG+YHLF+Q+NP GAV+   +VWAHS S DLINW 
Sbjct: 49  QPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWG-NIVWAHSTSTDLINWD 108

Query: 106 HLNPALEPTHPFDINGCWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEK 165
              PA+ P+ PFDINGCWSGSA+ LP  KPVILYTGID    QVQN+A P N SDP+L +
Sbjct: 109 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 168

Query: 166 WTKFPNNPIISPP--DHVLGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDF 225
           W K P NP+++P   + +    FRDPTTAW G D  WRV IG +     G A+ Y S+DF
Sbjct: 169 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKI-HRRGLAITYTSKDF 228

Query: 226 IRWNLLRFPLYSSPDSGTWECPDFFPVLLNGTNGVDFSSGFGIG---VKHVMKASFN--S 285
           ++W     PL+    SG WECPDFFPV   G+NGV+ SS FG     +KHV+K S +   
Sbjct: 229 LKWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS-FGEPNEILKHVLKISLDDTK 288

Query: 286 HDYYTLGSYAPEEEKFTPDSGSDFGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWV 345
           HDYYT+G+Y   ++KF PD+    GF+      RYDYGK+YASKTF+DS++ RRILWGW 
Sbjct: 289 HDYYTIGTYDRVKDKFVPDN----GFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWT 348

Query: 346 NESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLIQWPVEEIKALRRNHF-SLNHKELIGR 405
           NES S EDD++KGWSG+Q +PRKIWL ++G+QLIQWPV E++ LR     +L +K L   
Sbjct: 349 NESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSG 408

Query: 406 STMKVPGGSASQVDAEVSFEFPYLEEAERMDTRWVDPQLLCSERNASVNGRVGPFGLLVL 465
           S ++V G +A+Q D EV F+   LE+A+ ++  W DPQL+CS+ N SV   +GPFGL+VL
Sbjct: 409 SRLEVYGVTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVL 468

Query: 466 ASNDLSEQTAVFFHILKAHK---RYVVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKI 525
           AS +L E T+V+F I KA +   +YVVLMCSDQSRSS +   DKTTYG+FVDI+P H+ +
Sbjct: 469 ASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPL 528

Query: 526 SLRTLVDHSVVESFAGEGKTCITSRVYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSK 576
           SLR L+DHSVVESF G+G+ CITSRVYP LA+ K + L+AFN G QSV + +LN W+M+ 
Sbjct: 529 SLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNS 584

BLAST of MC08g0502 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.6e-170
Identity = 286/536 (53.36%), Postives = 378/536 (70.52%), Query Frame = 0

Query: 49  RTSYHFQPPKNWM---NGPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLN 108
           RT+YHFQ PKNWM   NGPM YKG+YHLF+QY P   V++ ++VW HS S DLINW    
Sbjct: 56  RTAYHFQSPKNWMNDPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQP 115

Query: 109 PALEPTHPFDINGCWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTK 168
            AL P+ P+DINGCWSGS + LP NKPVILYTGI++   QVQNLA+P N SDP+L++W K
Sbjct: 116 IALSPSEPYDINGCWSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIK 175

Query: 169 FPNNPIISPP----DHVLGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFI 228
            P NP+++      +++    FRDP+TAW+  DG WRV +G Q     G A+L+ S+DF+
Sbjct: 176 LPQNPLMAGTPTNNNNINASSFRDPSTAWQLSDGKWRVIVGTQQGK-RGLAVLFTSDDFV 235

Query: 229 RWNLLRFPLYSSPDSGTWECPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASF--NSHDYY 288
           +WN    PL+S+  +G WECPDFFPV +  + G D +S  G  VKHV+K S     ++YY
Sbjct: 236 KWNNTGNPLHSTEGNGIWECPDFFPVYVGKSLGAD-TSIIGDDVKHVLKLSLFDTQYEYY 295

Query: 289 TLGSYAPEEEKFTPDSGSDFGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESD 348
           T+G Y  E++ + PD GS      ++LGLRYDYGKFYASK+F+D    RRILWGWVNES 
Sbjct: 296 TIGRYDIEKDIYVPDEGS----IESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESS 355

Query: 349 SREDDIKKGWSGLQAVPRKIWLSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKV 408
            + DDIKKGWSG+QA+PR + L K+G+QL+QWP+ E+  LR N   L  + + G S +++
Sbjct: 356 IQADDIKKGWSGVQAIPRTVVLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEI 415

Query: 409 PGGSASQVDAEVSFEFPYLEEAERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDL 468
              +ASQ D E+SF+ P     E +D+   +PQ+LCS++ AS+ GR GPFGLL LAS  L
Sbjct: 416 SQITASQADVEISFKIPESNYVEELDSTCTNPQILCSQKGASIKGRFGPFGLLTLASMGL 475

Query: 469 SEQTAVFFHILKAHKRYVVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDH 528
            E TAVFF I K   +YVVLMCSDQ+RSS     DK ++G FVD+DP +E +SLR L+DH
Sbjct: 476 EEYTAVFFRIFKGPNKYVVLMCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDH 535

Query: 529 SVVESFAGEGKTCITSRVYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQIN 576
           S+VESF+ +GK+CIT+RVYPT+A++  A+LY FNNGT+ V I+ L+ W+M KAQIN
Sbjct: 536 SIVESFSAKGKSCITARVYPTMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQIN 585

BLAST of MC08g0502 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 581.3 bits (1497), Expect = 1.3e-164
Identity = 290/544 (53.31%), Postives = 369/544 (67.83%), Query Frame = 0

Query: 42  HIPQQALRTSYHFQPPKNWM---NGPMYYKGVYHLFHQYNPNGAVFN-QKMVWAHSISLD 101
           +I  Q  RT YHFQPPKNWM   NGPM YKG+YHLF+Q+N NGAV +  K VW H+ S D
Sbjct: 36  NIKNQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTD 95

Query: 102 LINWVHLNPALEPTHPFDINGCWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSD 161
           LINW+ L+PA+ P+ P DINGCWSGS + LP  KPVILYTG D    QVQNL  P N +D
Sbjct: 96  LINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTD 155

Query: 162 PFLEKWTKFPNNPII--SPPDHVLGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLY 221
           P+L  WTK P NP++  SP +H+    FRDPTTAW G DG WR+  G Q     G A+L+
Sbjct: 156 PYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQ-EGRRGLAILH 215

Query: 222 RSEDFIRWNLLRFPLYSSPDSGTWECPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNS 281
            S+DF+ W     PL+    +G WECPDFFPV    + G+D S   G  VKHV+K S   
Sbjct: 216 TSKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTD 275

Query: 282 --HDYYTLGSYAPEEEKFTPDSGSDFGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWG 341
             +DYYT+G+Y    + + PD     GF       RYDYGKFYASKTFYDS  +RRILWG
Sbjct: 276 TFNDYYTIGTYDEVRDVYVPDK----GFVQDETAPRYDYGKFYASKTFYDSVNQRRILWG 335

Query: 342 WVNESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLIQWPVEEIKALRRNHFSLNHKELIG 401
           WVNES   +D+IKKGW+GLQA+PRK+WL ++G++L+QWPV+EI+ LR       +K L G
Sbjct: 336 WVNESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKG 395

Query: 402 RSTMKVPGGSASQVDAEVSFEFP--YLEEAERMDTRWVDPQLLCSERNASVNGRVGPFGL 461
            S M+V G +A Q D EV F+     LE+A+ ++  W DPQL+CS+RNAS    +GPFGL
Sbjct: 396 GSVMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNAS--SGLGPFGL 455

Query: 462 LVLASNDLSEQTAVFFHILKA---HKRYVVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRH 521
           +VLAS ++ E T+V   I +A    K +VV+MCSDQS SS     DKTTYG+F+DI P +
Sbjct: 456 MVLASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP-Y 515

Query: 522 EKISLRTLVDHSVVESFAGEGKTCITSRVYPTLAVDKDARLYAFNNGTQSVVISSLNVWN 573
           + ISLRTL+D S+VESF G+GKTCITSRVYP LA+ +   L+AFN G+Q+V + SL+ W+
Sbjct: 516 QPISLRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWS 571

BLAST of MC08g0502 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.9e-161
Identity = 280/525 (53.33%), Postives = 357/525 (68.00%), Query Frame = 0

Query: 34  SHGQTNELHIPQQALRTSYHFQPPKNWM---NGPMYYKGVYHLFHQYNPNGAVFNQKMVW 93
           SH  +   H   Q  RT+YHFQP KNW+   NGPM Y G YHLF+QYNP GAV+   +VW
Sbjct: 35  SHHSSKSNH---QPYRTAYHFQPLKNWINDPNGPMRYGGFYHLFYQYNPKGAVWG-NIVW 94

Query: 94  AHSISLDLINWVHLNPALEPTHPFDINGCWSGSASFLPGNKPVILYTGIDSSFNQVQNLA 153
           AHS+S DL+NW  L+ A+ P+ P DI GCWSGSA+ LPG KP ILYTGID + +QVQN+A
Sbjct: 95  AHSVSKDLVNWTPLDHAIHPSQPSDIKGCWSGSATILPGGKPAILYTGIDPNNHQVQNIA 154

Query: 154 IPANFSDPFLEKWTKFPNNPIISP--PDHVLGDRFRDPTTAWRGPDGGWRVAIGGQTSDG 213
           IP N SDP L +W K P NP++ P   + +    FRDPTT+W G DG WRV IG +  D 
Sbjct: 155 IPKNMSDPLLREWKKSPKNPLMEPTIANKINSSSFRDPTTSWLGKDGFWRVLIGSKI-DT 214

Query: 214 GGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPDFFPVLLNG--TNGVDFSSGFGIGVK 273
            G A+LY+S++F+ W   + PL+S+  +G WECPDF+PVL       GVD S      V+
Sbjct: 215 KGMAILYKSKNFVDWVEAKHPLHSAEGTGMWECPDFYPVLDKNLLRTGVDTSRNGDDDVR 274

Query: 274 HVMKASFN--SHDYYTLGSYAPEEEKFTPDSGSDFGFRGTNLGLRYDYGKFYASKTFYDS 333
           HV+K S +   HD+Y +GSY   ++ F P++    GF      LRYDYGK+YASKTF+D 
Sbjct: 275 HVLKVSLDDTKHDHYLIGSYDVVKDVFVPEN----GFEDNGFVLRYDYGKYYASKTFFDD 334

Query: 334 SQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLIQWPVEEIKALRRNHF 393
            + RRIL GWVNES S  DD+KKGWSG+  +PR+IWL ++G+QL+QWPV+EI+ LR N  
Sbjct: 335 GKNRRILLGWVNESSSVADDVKKGWSGIHTIPREIWLHESGKQLVQWPVKEIENLRMNPV 394

Query: 394 SLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAERMDTRWVDPQLLCSERNASVNG 453
           +   K + G   + + G  + Q D E+SFE   L + E +  +W+DPQLLCS++ A V G
Sbjct: 395 NWPTKVIKGGERISITGVDSVQADVEISFEISDLGKVESL-RKWIDPQLLCSQKGAGVKG 454

Query: 454 RVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLMCSDQSRSSFRNRVDKTTYGSFVDI 513
            VGPFGLLV AS  L E TAVFF I K   + +VLMCSDQSRSS     D T+YG+FVD+
Sbjct: 455 GVGPFGLLVFASQGLKEYTAVFFRIFKYQDKNLVLMCSDQSRSSLNKDNDMTSYGTFVDV 514

Query: 514 DPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPTLAVDKDARL 550
           DP HEK+SLRTL+DHSVVESF GEG+ C+T+RVYPTLA+   A L
Sbjct: 515 DPLHEKLSLRTLIDHSVVESFGGEGRACVTARVYPTLAIHDKAML 549

BLAST of MC08g0502 vs. NCBI nr
Match: XP_022131914.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X2 [Momordica charantia])

HSP 1 Score: 1209 bits (3127), Expect = 0.0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 4   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMNG 63
           MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMNG
Sbjct: 1   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMNG 60

Query: 64  PMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGS 123
           PMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGS
Sbjct: 61  PMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGS 120

Query: 124 ASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLGDRF 183
           ASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLGDRF
Sbjct: 121 ASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLGDRF 180

Query: 184 RDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPD 243
           RDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPD
Sbjct: 181 RDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPD 240

Query: 244 FFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFGFRG 303
           FFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFGFRG
Sbjct: 241 FFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFGFRG 300

Query: 304 TNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSK 363
           TNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSK
Sbjct: 301 TNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSK 360

Query: 364 TGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAER 423
           TGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAER
Sbjct: 361 TGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAER 420

Query: 424 MDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLMCSD 483
           MDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLMCSD
Sbjct: 421 MDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLMCSD 480

Query: 484 QSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPTLAV 543
           QSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPTLAV
Sbjct: 481 QSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPTLAV 540

Query: 544 DKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP 579
           DKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP
Sbjct: 541 DKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP 576

BLAST of MC08g0502 vs. NCBI nr
Match: XP_022131913.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X1 [Momordica charantia])

HSP 1 Score: 1203 bits (3113), Expect = 0.0
Identity = 576/579 (99.48%), Postives = 576/579 (99.48%), Query Frame = 0

Query: 4   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMN- 63
           MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMN 
Sbjct: 1   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMND 60

Query: 64  --GPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCW 123
             GPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCW
Sbjct: 61  PNGPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCW 120

Query: 124 SGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLG 183
           SGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLG
Sbjct: 121 SGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLG 180

Query: 184 DRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWE 243
           DRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWE
Sbjct: 181 DRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWE 240

Query: 244 CPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFG 303
           CPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFG
Sbjct: 241 CPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFG 300

Query: 304 FRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW 363
           FRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW
Sbjct: 301 FRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW 360

Query: 364 LSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEE 423
           LSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEE
Sbjct: 361 LSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEE 420

Query: 424 AERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLM 483
           AERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLM
Sbjct: 421 AERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLM 480

Query: 484 CSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPT 543
           CSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPT
Sbjct: 481 CSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPT 540

Query: 544 LAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP 579
           LAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP
Sbjct: 541 LAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP 579

BLAST of MC08g0502 vs. NCBI nr
Match: XP_008444460.1 (PREDICTED: LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo])

HSP 1 Score: 959 bits (2480), Expect = 0.0
Identity = 460/578 (79.58%), Postives = 507/578 (87.72%), Query Frame = 0

Query: 3   EMPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELH-IPQQALRTSYHFQPPKNWM 62
           +MP K L+MGK+  V   ++ L SN +GFVVSH   NE+H  PQQ  RTSYHFQP KNWM
Sbjct: 3   KMPPKRLEMGKYGLVASFILLLASNRMGFVVSHEYANEVHSTPQQVQRTSYHFQPLKNWM 62

Query: 63  N---GPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDING 122
           N   GPM+YKG+YH F+Q+NPNGAVFN KMVWAHSIS DLINWVHLN ALEPT PFDING
Sbjct: 63  NDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDING 122

Query: 123 CWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHV 182
           CWSGS SFLP NKP ILYTGIDSSF QVQNLAIP N+SDPFLEKW K P NPII+PPD  
Sbjct: 123 CWSGSVSFLPKNKPRILYTGIDSSFQQVQNLAIPTNYSDPFLEKWRKSPQNPIIAPPDGA 182

Query: 183 LGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGT 242
             +RFRDPTTAW+GPDG WRVAIGGQTS GG AAMLYRSEDF+RW+  RFPLYSS DSGT
Sbjct: 183 ERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG-AAMLYRSEDFVRWHQFRFPLYSSQDSGT 242

Query: 243 WECPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSD 302
           WECPDF+PV+LNGTNG+ FSSGFG+GVKHVMKASFNS D+YTLGSY PE+EKFT D G  
Sbjct: 243 WECPDFYPVMLNGTNGIGFSSGFGVGVKHVMKASFNSRDHYTLGSYVPEKEKFTSDHGLG 302

Query: 303 FGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRK 362
           F FRG NLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSR+DDI KGWSGLQA+PRK
Sbjct: 303 FDFRGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 362

Query: 363 IWLSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYL 422
           IWLSKTGRQLIQWPV+EIK LRRNHFSL+HKEL GRSTM+V GGSASQVD EVSF  P+L
Sbjct: 363 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFALPHL 422

Query: 423 EEAERMDTRW--VDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRY 482
           EEAE +DTRW  +DPQ+LCS+R+AS+NGRVGPFGLLVLASNDLSE TA++FH+LKAH RY
Sbjct: 423 EEAESVDTRWGVLDPQVLCSKRDASMNGRVGPFGLLVLASNDLSEHTAIYFHVLKAHNRY 482

Query: 483 VVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSR 542
           VVLMCSD+SRSSFR  VDKTTYG+FVDIDPR++KISLRTLVDHS+VESF G+GKTCITSR
Sbjct: 483 VVLMCSDESRSSFRKGVDKTTYGAFVDIDPRYKKISLRTLVDHSIVESFGGKGKTCITSR 542

Query: 543 VYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQI 574
           VYPTLAV+ DARLYAFNNGTQ+VVISSL VWNMS A+I
Sbjct: 543 VYPTLAVNNDARLYAFNNGTQTVVISSLKVWNMSNAEI 579

BLAST of MC08g0502 vs. NCBI nr
Match: XP_023545584.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 954 bits (2467), Expect = 0.0
Identity = 457/574 (79.62%), Postives = 501/574 (87.28%), Query Frame = 0

Query: 4   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMNG 63
           M TKWL+M K   V L  V+L+SN  GFVVSHG  NE+HIPQQ  RTSYHFQP +NWMNG
Sbjct: 1   MATKWLEMVKCGVVALYFVWLISNRFGFVVSHGDINEVHIPQQFQRTSYHFQPLQNWMNG 60

Query: 64  PMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGS 123
           PMYYKG+YHLF+Q+NPNGAVF+ K+VW H++S DLINW HLN ALEPTHPFDINGCWSGS
Sbjct: 61  PMYYKGIYHLFYQHNPNGAVFDSKIVWGHAVSRDLINWAHLNHALEPTHPFDINGCWSGS 120

Query: 124 ASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLGDRF 183
            SFL  NKPVILYTG+DS F QVQNLAIP+NFSDPFLE WTK  +NPII+PP+ +   RF
Sbjct: 121 VSFLHNNKPVILYTGLDSGFQQVQNLAIPSNFSDPFLEIWTKSSHNPIITPPNGIERHRF 180

Query: 184 RDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPD 243
           RDPTTAW GPDGGWR+AIGGQTS GG AAMLYRSEDF+ W+  RFPLYSS DSG WECPD
Sbjct: 181 RDPTTAWPGPDGGWRIAIGGQTSYGG-AAMLYRSEDFVHWHQSRFPLYSSRDSGNWECPD 240

Query: 244 FFPVLLNGTNGVDFSSGFGIG-VKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFGFR 303
           F+PV LNG+NG+D  +GF +G VK+VMKASFNSHDYYTLGSY PE+EKFT DSG  F  R
Sbjct: 241 FYPVKLNGSNGIDSLNGFRVGAVKYVMKASFNSHDYYTLGSYVPEKEKFTSDSGDGFDLR 300

Query: 304 GTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLS 363
           GTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRED+IKKGW+GLQ VPRKIWLS
Sbjct: 301 GTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDNIKKGWAGLQVVPRKIWLS 360

Query: 364 KTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAE 423
           K GRQLIQWPV+E++ LR N FSL +KEL GRSTMKV GGSASQVDAEVSFEFPYLEEAE
Sbjct: 361 KNGRQLIQWPVKEVEKLRTNRFSLYNKELRGRSTMKVLGGSASQVDAEVSFEFPYLEEAE 420

Query: 424 RMDTRW--VDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLM 483
           R+DTRW  +DPQLLCS+R ASVNGRVGPFGLLVLASNDLSE TA+FFH+LKAH RYVVLM
Sbjct: 421 RVDTRWFDLDPQLLCSKRGASVNGRVGPFGLLVLASNDLSEHTAIFFHVLKAHNRYVVLM 480

Query: 484 CSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPT 543
           CSDQSRSSFR  VDKTTYG+FVD+DPRHEKISLRTLVD S+VESF G+GKTCITSRVYPT
Sbjct: 481 CSDQSRSSFREGVDKTTYGAFVDVDPRHEKISLRTLVDRSIVESFGGKGKTCITSRVYPT 540

Query: 544 LAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQI 574
           +AVD DARLYAFNNGTQSVVIS L VW MS AQI
Sbjct: 541 VAVDDDARLYAFNNGTQSVVISRLKVWKMSNAQI 573

BLAST of MC08g0502 vs. NCBI nr
Match: XP_011649529.2 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X2 [Cucumis sativus])

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 457/576 (79.34%), Postives = 505/576 (87.67%), Query Frame = 0

Query: 1   FHEMPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNW 60
           F +MP K L++GK+  V   ++ L  N IGFVVSH   NE+H PQ   RTSYHFQP KNW
Sbjct: 30  FDKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQ-VQRTSYHFQPLKNW 89

Query: 61  MNGPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCW 120
           MNGPM+YKG+YH F+Q+NPNGAVFN KMVWAHSIS DLINWVHLN ALEPT PFDINGCW
Sbjct: 90  MNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCW 149

Query: 121 SGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLG 180
           SGS SFLP NKP ILYTGIDSS  QVQNLAIPAN+SDPFLEKW KF  NPII+PPD +  
Sbjct: 150 SGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLER 209

Query: 181 DRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWE 240
           +RFRDPTTAW+GPDG WRVAIGGQTS GG AAMLYRSEDF+RW+  RFPLYSS DSGTWE
Sbjct: 210 NRFRDPTTAWQGPDGEWRVAIGGQTSYGG-AAMLYRSEDFVRWHQFRFPLYSSQDSGTWE 269

Query: 241 CPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFG 300
           CPDF+PV+LNGTNG+ FSSGFG+GVK+VMKASFNS D+YTLGSY PE+EKFT D G  F 
Sbjct: 270 CPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFD 329

Query: 301 FRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW 360
           F+G NLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSR+DDI KGWSGLQA+PRKIW
Sbjct: 330 FKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIW 389

Query: 361 LSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEE 420
           LSKTGRQLIQWPV+EIK LRRNHFSL+HKEL GRSTM+V GGSASQVD EVSFE PYLEE
Sbjct: 390 LSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEE 449

Query: 421 AERMDTRW--VDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVV 480
           AE +DTRW  +DPQ+LCS+R+ASVNGRVGPFGLLVLASNDLSE TA++FHILKAH RYVV
Sbjct: 450 AESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVV 509

Query: 481 LMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVY 540
           LMCSDQSRSSFR  VDKT +G+FVDIDP ++KISLRTLVDHS+VESF G+GKTCITSRVY
Sbjct: 510 LMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVY 569

Query: 541 PTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQI 574
           PTLAV+ +ARLYAFNNGTQ+VVISSL  WNMS A+I
Sbjct: 570 PTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEI 603

BLAST of MC08g0502 vs. ExPASy TrEMBL
Match: A0A6J1BRK6 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004934 PE=3 SV=1)

HSP 1 Score: 1209 bits (3127), Expect = 0.0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 4   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMNG 63
           MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMNG
Sbjct: 1   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMNG 60

Query: 64  PMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGS 123
           PMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGS
Sbjct: 61  PMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGS 120

Query: 124 ASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLGDRF 183
           ASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLGDRF
Sbjct: 121 ASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLGDRF 180

Query: 184 RDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPD 243
           RDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPD
Sbjct: 181 RDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPD 240

Query: 244 FFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFGFRG 303
           FFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFGFRG
Sbjct: 241 FFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFGFRG 300

Query: 304 TNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSK 363
           TNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSK
Sbjct: 301 TNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSK 360

Query: 364 TGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAER 423
           TGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAER
Sbjct: 361 TGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAER 420

Query: 424 MDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLMCSD 483
           MDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLMCSD
Sbjct: 421 MDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLMCSD 480

Query: 484 QSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPTLAV 543
           QSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPTLAV
Sbjct: 481 QSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPTLAV 540

Query: 544 DKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP 579
           DKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP
Sbjct: 541 DKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP 576

BLAST of MC08g0502 vs. ExPASy TrEMBL
Match: A0A6J1BUT2 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004934 PE=3 SV=1)

HSP 1 Score: 1203 bits (3113), Expect = 0.0
Identity = 576/579 (99.48%), Postives = 576/579 (99.48%), Query Frame = 0

Query: 4   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMN- 63
           MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMN 
Sbjct: 1   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMND 60

Query: 64  --GPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCW 123
             GPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCW
Sbjct: 61  PNGPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCW 120

Query: 124 SGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLG 183
           SGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLG
Sbjct: 121 SGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLG 180

Query: 184 DRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWE 243
           DRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWE
Sbjct: 181 DRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWE 240

Query: 244 CPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFG 303
           CPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFG
Sbjct: 241 CPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFG 300

Query: 304 FRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW 363
           FRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW
Sbjct: 301 FRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW 360

Query: 364 LSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEE 423
           LSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEE
Sbjct: 361 LSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEE 420

Query: 424 AERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLM 483
           AERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLM
Sbjct: 421 AERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLM 480

Query: 484 CSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPT 543
           CSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPT
Sbjct: 481 CSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPT 540

Query: 544 LAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP 579
           LAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP
Sbjct: 541 LAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQINENQP 579

BLAST of MC08g0502 vs. ExPASy TrEMBL
Match: A0A1S3BB78 (LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like OS=Cucumis melo OX=3656 GN=LOC103487780 PE=3 SV=1)

HSP 1 Score: 959 bits (2480), Expect = 0.0
Identity = 460/578 (79.58%), Postives = 507/578 (87.72%), Query Frame = 0

Query: 3   EMPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELH-IPQQALRTSYHFQPPKNWM 62
           +MP K L+MGK+  V   ++ L SN +GFVVSH   NE+H  PQQ  RTSYHFQP KNWM
Sbjct: 3   KMPPKRLEMGKYGLVASFILLLASNRMGFVVSHEYANEVHSTPQQVQRTSYHFQPLKNWM 62

Query: 63  N---GPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDING 122
           N   GPM+YKG+YH F+Q+NPNGAVFN KMVWAHSIS DLINWVHLN ALEPT PFDING
Sbjct: 63  NDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDING 122

Query: 123 CWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHV 182
           CWSGS SFLP NKP ILYTGIDSSF QVQNLAIP N+SDPFLEKW K P NPII+PPD  
Sbjct: 123 CWSGSVSFLPKNKPRILYTGIDSSFQQVQNLAIPTNYSDPFLEKWRKSPQNPIIAPPDGA 182

Query: 183 LGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGT 242
             +RFRDPTTAW+GPDG WRVAIGGQTS GG AAMLYRSEDF+RW+  RFPLYSS DSGT
Sbjct: 183 ERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG-AAMLYRSEDFVRWHQFRFPLYSSQDSGT 242

Query: 243 WECPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSD 302
           WECPDF+PV+LNGTNG+ FSSGFG+GVKHVMKASFNS D+YTLGSY PE+EKFT D G  
Sbjct: 243 WECPDFYPVMLNGTNGIGFSSGFGVGVKHVMKASFNSRDHYTLGSYVPEKEKFTSDHGLG 302

Query: 303 FGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRK 362
           F FRG NLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSR+DDI KGWSGLQA+PRK
Sbjct: 303 FDFRGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 362

Query: 363 IWLSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYL 422
           IWLSKTGRQLIQWPV+EIK LRRNHFSL+HKEL GRSTM+V GGSASQVD EVSF  P+L
Sbjct: 363 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFALPHL 422

Query: 423 EEAERMDTRW--VDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRY 482
           EEAE +DTRW  +DPQ+LCS+R+AS+NGRVGPFGLLVLASNDLSE TA++FH+LKAH RY
Sbjct: 423 EEAESVDTRWGVLDPQVLCSKRDASMNGRVGPFGLLVLASNDLSEHTAIYFHVLKAHNRY 482

Query: 483 VVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSR 542
           VVLMCSD+SRSSFR  VDKTTYG+FVDIDPR++KISLRTLVDHS+VESF G+GKTCITSR
Sbjct: 483 VVLMCSDESRSSFRKGVDKTTYGAFVDIDPRYKKISLRTLVDHSIVESFGGKGKTCITSR 542

Query: 543 VYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQI 574
           VYPTLAV+ DARLYAFNNGTQ+VVISSL VWNMS A+I
Sbjct: 543 VYPTLAVNNDARLYAFNNGTQTVVISSLKVWNMSNAEI 579

BLAST of MC08g0502 vs. ExPASy TrEMBL
Match: A0A6J1HFM6 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462607 PE=3 SV=1)

HSP 1 Score: 954 bits (2465), Expect = 0.0
Identity = 457/574 (79.62%), Postives = 502/574 (87.46%), Query Frame = 0

Query: 4   MPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWMNG 63
           M TKW++M K   V L  V+L+SN  GFVVSHG  NE+HIPQQ  RTSYHFQP +NWMNG
Sbjct: 1   MATKWMEMVKCGLVALYFVWLISNRFGFVVSHGDINEVHIPQQFQRTSYHFQPLQNWMNG 60

Query: 64  PMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGS 123
           PMYYKG+YHLF+Q+NPNGAVF+ K+VW H++S DLINW HLN ALEPTHPFDINGCWSGS
Sbjct: 61  PMYYKGIYHLFYQHNPNGAVFDSKIVWGHAVSRDLINWAHLNHALEPTHPFDINGCWSGS 120

Query: 124 ASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDHVLGDRF 183
            SFL  NKPVILYTG+DSSF QVQNLAIP+NFSDPFLE WTK  +NPII+PP+ +   RF
Sbjct: 121 VSFLHNNKPVILYTGLDSSFQQVQNLAIPSNFSDPFLEIWTKSSHNPIIAPPNGIERHRF 180

Query: 184 RDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPD 243
           RDPTTAW GPDGGWR+ IGGQTS GG AAMLYRSEDF+ W+  RFPLYSS DSG WECPD
Sbjct: 181 RDPTTAWPGPDGGWRIVIGGQTSYGG-AAMLYRSEDFVHWHQSRFPLYSSRDSGNWECPD 240

Query: 244 FFPVLLNGTNGVDFSSGFGIG-VKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGSDFGFR 303
           F+PV LNG+NG+D  +GF +G VK+VMKASFNSHDYYTLGSY PE+EKFT DSG  F  R
Sbjct: 241 FYPVKLNGSNGIDSLNGFRVGAVKYVMKASFNSHDYYTLGSYVPEKEKFTSDSGDGFDLR 300

Query: 304 GTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLS 363
           GTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRED+IKKGW+GLQ VPRKIWLS
Sbjct: 301 GTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDNIKKGWAGLQVVPRKIWLS 360

Query: 364 KTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAE 423
           KTGRQLIQWPV+E++ LR N FSL +KEL GRSTMKV GGSASQVDAEVSFEFPYLEEAE
Sbjct: 361 KTGRQLIQWPVKEVEKLRTNRFSLYNKELRGRSTMKVLGGSASQVDAEVSFEFPYLEEAE 420

Query: 424 RMDTRW--VDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKRYVVLM 483
           R+DTRW  +DPQLLCS+R+ASVNGRVGPFGLLVLASNDLSE TA+FF +LKAH RYVVLM
Sbjct: 421 RVDTRWFDLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFFRVLKAHNRYVVLM 480

Query: 484 CSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRVYPT 543
           CSDQSRSSFR  VDKTTYG+FVD+DPRHEKISLRTLVD S+VESF G+GKTCITSRVYPT
Sbjct: 481 CSDQSRSSFREGVDKTTYGAFVDVDPRHEKISLRTLVDRSIVESFGGKGKTCITSRVYPT 540

Query: 544 LAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQI 574
           LAVD DARLYAFNNGTQSVVISSL VW M  AQI
Sbjct: 541 LAVDDDARLYAFNNGTQSVVISSLKVWKMWNAQI 573

BLAST of MC08g0502 vs. ExPASy TrEMBL
Match: A0A0A0LQX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351670 PE=3 SV=1)

HSP 1 Score: 949 bits (2452), Expect = 0.0
Identity = 457/579 (78.93%), Postives = 505/579 (87.22%), Query Frame = 0

Query: 1   FHEMPTKWLKMGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNW 60
           F +MP K L++GK+  V   ++ L  N IGFVVSH   NE+H PQ   RTSYHFQP KNW
Sbjct: 19  FDKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTPQ-VQRTSYHFQPLKNW 78

Query: 61  MN---GPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDIN 120
           MN   GPM+YKG+YH F+Q+NPNGAVFN KMVWAHSIS DLINWVHLN ALEPT PFDIN
Sbjct: 79  MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN 138

Query: 121 GCWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPIISPPDH 180
           GCWSGS SFLP NKP ILYTGIDSS  QVQNLAIPAN+SDPFLEKW KF  NPII+PPD 
Sbjct: 139 GCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDG 198

Query: 181 VLGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSG 240
           +  +RFRDPTTAW+GPDG WRVAIGGQTS GG AAMLYRSEDF+RW+  RFPLYSS DSG
Sbjct: 199 LERNRFRDPTTAWQGPDGEWRVAIGGQTSYGG-AAMLYRSEDFVRWHQFRFPLYSSQDSG 258

Query: 241 TWECPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASFNSHDYYTLGSYAPEEEKFTPDSGS 300
           TWECPDF+PV+LNGTNG+ FSSGFG+GVK+VMKASFNS D+YTLGSY PE+EKFT D G 
Sbjct: 259 TWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGP 318

Query: 301 DFGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPR 360
            F F+G NLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSR+DDI KGWSGLQA+PR
Sbjct: 319 GFDFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPR 378

Query: 361 KIWLSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPY 420
           KIWLSKTGRQLIQWPV+EIK LRRNHFSL+HKEL GRSTM+V GGSASQVD EVSFE PY
Sbjct: 379 KIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPY 438

Query: 421 LEEAERMDTRW--VDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAHKR 480
           LEEAE +DTRW  +DPQ+LCS+R+ASVNGRVGPFGLLVLASNDLSE TA++FHILKAH R
Sbjct: 439 LEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNR 498

Query: 481 YVVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITS 540
           YVVLMCSDQSRSSFR  VDKT +G+FVDIDP ++KISLRTLVDHS+VESF G+GKTCITS
Sbjct: 499 YVVLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITS 558

Query: 541 RVYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQI 574
           RVYPTLAV+ +ARLYAFNNGTQ+VVISSL  WNMS A+I
Sbjct: 559 RVYPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEI 595

BLAST of MC08g0502 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 632.1 bits (1629), Expect = 4.4e-181
Identity = 305/578 (52.77%), Postives = 399/578 (69.03%), Query Frame = 0

Query: 11  MGKFDFVGLCLVFLLSNGIGFVVSHGQTNELHIPQQALRTSYHFQPPKNWM---NGPMYY 70
           M K +   + L  LLS  +   ++  + +      Q  RT YHFQP KNWM   NGPM Y
Sbjct: 1   MAKLNRSNIGLSLLLSMFLANFITDLEASSHQDLNQPYRTGYHFQPLKNWMNDPNGPMIY 60

Query: 71  KGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSGSASFL 130
           KG+YHLF+QYNP GAV++ ++VW HS S+DL+NW+   PA  P+ P DINGCWSGS + L
Sbjct: 61  KGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTIL 120

Query: 131 PGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPII--SPPDHVLGDRFRD 190
           P  KPVILYTGID +  QVQN+A+P N SDP+L +W+K P NP++  +  + +  DRFRD
Sbjct: 121 PNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRD 180

Query: 191 PTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWECPDFF 250
           PTTAW G DG WRV +G  T D  G A+LY+S DF  W     PL+    +G WECPDFF
Sbjct: 181 PTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFF 240

Query: 251 PVLLNGTNGVDFSSGFGIGVKHVMKASF--NSHDYYTLGSYAPEEEKFTPDSGSDFGFRG 310
           PV + G++GV+ SS    G+KHV+K S     HDYYT+GSY  E++ + P    D GF  
Sbjct: 241 PVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVP----DLGFVQ 300

Query: 311 TNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSK 370
                R DYGK+YASKTFYD  +KRRILWGWVNES   +DDI+KGWSGLQ+ PRKIWL +
Sbjct: 301 NESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDE 360

Query: 371 TGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYLEEAER 430
           +G++L+QWP+EEI+ LR    +   K L   ST++V G +A+Q D EVSF+   LE+A+ 
Sbjct: 361 SGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADV 420

Query: 431 MDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAH------KRYV 490
           ++  W DPQ +CS+ + SV   +GPFGL+VLASND+ E T+V+F I K++       +YV
Sbjct: 421 IEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYV 480

Query: 491 VLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTCITSRV 550
           VLMCSDQSRSS  +  DK+T+G+FV IDP H+ ISLRTL+DHS+VES+ G G+TCITSRV
Sbjct: 481 VLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRV 540

Query: 551 YPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQIN 576
           YP LA+ ++A L+ FN GTQSV I +L+ W++  AQIN
Sbjct: 541 YPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of MC08g0502 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 611.7 bits (1576), Expect = 6.1e-175
Identity = 303/541 (56.01%), Postives = 386/541 (71.35%), Query Frame = 0

Query: 46  QALRTSYHFQPPKNWMNGPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLN 105
           Q  RT +HFQPPKNWMNGPM YKG+YHLF+Q+NP GAV+   +VWAHS S DLINW    
Sbjct: 49  QPYRTGFHFQPPKNWMNGPMIYKGIYHLFYQWNPKGAVWG-NIVWAHSTSTDLINWDPHP 108

Query: 106 PALEPTHPFDINGCWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTK 165
           PA+ P+ PFDINGCWSGSA+ LP  KPVILYTGID    QVQN+A P N SDP+L +W K
Sbjct: 109 PAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKK 168

Query: 166 FPNNPIISPP--DHVLGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRW 225
            P NP+++P   + +    FRDPTTAW G D  WRV IG +     G A+ Y S+DF++W
Sbjct: 169 SPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKI-HRRGLAITYTSKDFLKW 228

Query: 226 NLLRFPLYSSPDSGTWECPDFFPVLLNGTNGVDFSSGFGIG---VKHVMKASFN--SHDY 285
                PL+    SG WECPDFFPV   G+NGV+ SS FG     +KHV+K S +   HDY
Sbjct: 229 EKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS-FGEPNEILKHVLKISLDDTKHDY 288

Query: 286 YTLGSYAPEEEKFTPDSGSDFGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNES 345
           YT+G+Y   ++KF PD+    GF+      RYDYGK+YASKTF+DS++ RRILWGW NES
Sbjct: 289 YTIGTYDRVKDKFVPDN----GFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNES 348

Query: 346 DSREDDIKKGWSGLQAVPRKIWLSKTGRQLIQWPVEEIKALRRNHF-SLNHKELIGRSTM 405
            S EDD++KGWSG+Q +PRKIWL ++G+QLIQWPV E++ LR     +L +K L   S +
Sbjct: 349 SSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRL 408

Query: 406 KVPGGSASQVDAEVSFEFPYLEEAERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASN 465
           +V G +A+Q D EV F+   LE+A+ ++  W DPQL+CS+ N SV   +GPFGL+VLAS 
Sbjct: 409 EVYGVTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASK 468

Query: 466 DLSEQTAVFFHILKAHK---RYVVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLR 525
           +L E T+V+F I KA +   +YVVLMCSDQSRSS +   DKTTYG+FVDI+P H+ +SLR
Sbjct: 469 NLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLR 528

Query: 526 TLVDHSVVESFAGEGKTCITSRVYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQI 576
            L+DHSVVESF G+G+ CITSRVYP LA+ K + L+AFN G QSV + +LN W+M+ AQI
Sbjct: 529 ALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQI 581

BLAST of MC08g0502 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 609.4 bits (1570), Expect = 3.1e-174
Identity = 289/541 (53.42%), Postives = 381/541 (70.43%), Query Frame = 0

Query: 45  QQALRTSYHFQPPKNWMNGPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHL 104
           + A+R  +    P   ++GPM YKG+YHLF+QYNP GAV++ ++VW HS S+DL+NW+  
Sbjct: 23  RHAVRRPFGHPVPNVSVSGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQ 82

Query: 105 NPALEPTHPFDINGCWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWT 164
            PA  P+ P DINGCWSGS + LP  KPVILYTGID +  QVQN+A+P N SDP+L +W+
Sbjct: 83  PPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWS 142

Query: 165 KFPNNPII--SPPDHVLGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIR 224
           K P NP++  +  + +  DRFRDPTTAW G DG WRV +G  T D  G A+LY+S DF  
Sbjct: 143 KPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFN 202

Query: 225 WNLLRFPLYSSPDSGTWECPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASF--NSHDYYT 284
           W     PL+    +G WECPDFFPV + G++GV+ SS    G+KHV+K S     HDYYT
Sbjct: 203 WTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYT 262

Query: 285 LGSYAPEEEKFTPDSGSDFGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDS 344
           +GSY  E++ + P    D GF       R DYGK+YASKTFYD  +KRRILWGWVNES  
Sbjct: 263 IGSYDREKDVYVP----DLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSP 322

Query: 345 REDDIKKGWSGLQAVPRKIWLSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVP 404
            +DDI+KGWSGLQ+ PRKIWL ++G++L+QWP+EEI+ LR    +   K L   ST++V 
Sbjct: 323 AKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVH 382

Query: 405 GGSASQVDAEVSFEFPYLEEAERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLS 464
           G +A+Q D EVSF+   LE+A+ ++  W DPQ +CS+ + SV   +GPFGL+VLASND+ 
Sbjct: 383 GVTAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDME 442

Query: 465 EQTAVFFHILKAH------KRYVVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLR 524
           E T+V+F I K++       +YVVLMCSDQSRSS  +  DK+T+G+FV IDP H+ ISLR
Sbjct: 443 EYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLR 502

Query: 525 TLVDHSVVESFAGEGKTCITSRVYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQI 576
           TL+DHS+VES+ G G+TCITSRVYP LA+ ++A L+ FN GTQSV I +L+ W++  AQI
Sbjct: 503 TLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 559

BLAST of MC08g0502 vs. TAIR 10
Match: AT1G55120.2 (beta-fructofuranosidase 5 )

HSP 1 Score: 606.3 bits (1562), Expect = 2.6e-173
Identity = 286/523 (54.68%), Postives = 373/523 (71.32%), Query Frame = 0

Query: 63  GPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWVHLNPALEPTHPFDINGCWSG 122
           GPM YKG+YHLF+QYNP GAV++ ++VW HS S+DL+NW+   PA  P+ P DINGCWSG
Sbjct: 17  GPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSG 76

Query: 123 SASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEKWTKFPNNPII--SPPDHVLG 182
           S + LP  KPVILYTGID +  QVQN+A+P N SDP+L +W+K P NP++  +  + +  
Sbjct: 77  SVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINP 136

Query: 183 DRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDFIRWNLLRFPLYSSPDSGTWE 242
           DRFRDPTTAW G DG WRV +G  T D  G A+LY+S DF  W     PL+    +G WE
Sbjct: 137 DRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWE 196

Query: 243 CPDFFPVLLNGTNGVDFSSGFGIGVKHVMKASF--NSHDYYTLGSYAPEEEKFTPDSGSD 302
           CPDFFPV + G++GV+ SS    G+KHV+K S     HDYYT+GSY  E++ + P    D
Sbjct: 197 CPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVP----D 256

Query: 303 FGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDDIKKGWSGLQAVPRK 362
            GF       R DYGK+YASKTFYD  +KRRILWGWVNES   +DDI+KGWSGLQ+ PRK
Sbjct: 257 LGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRK 316

Query: 363 IWLSKTGRQLIQWPVEEIKALRRNHFSLNHKELIGRSTMKVPGGSASQVDAEVSFEFPYL 422
           IWL ++G++L+QWP+EEI+ LR    +   K L   ST++V G +A+Q D EVSF+   L
Sbjct: 317 IWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKEL 376

Query: 423 EEAERMDTRWVDPQLLCSERNASVNGRVGPFGLLVLASNDLSEQTAVFFHILKAH----- 482
           E+A+ ++  W DPQ +CS+ + SV   +GPFGL+VLASND+ E T+V+F I K++     
Sbjct: 377 EKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNK 436

Query: 483 -KRYVVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKISLRTLVDHSVVESFAGEGKTC 542
             +YVVLMCSDQSRSS  +  DK+T+G+FV IDP H+ ISLRTL+DHS+VES+ G G+TC
Sbjct: 437 KTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTC 496

Query: 543 ITSRVYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSKAQIN 576
           ITSRVYP LA+ ++A L+ FN GTQSV I +L+ W++  AQIN
Sbjct: 497 ITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 535

BLAST of MC08g0502 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 606.3 bits (1562), Expect = 2.6e-173
Identity = 303/544 (55.70%), Postives = 386/544 (70.96%), Query Frame = 0

Query: 46  QALRTSYHFQPPKNWM---NGPMYYKGVYHLFHQYNPNGAVFNQKMVWAHSISLDLINWV 105
           Q  RT +HFQPPKNWM   NGPM YKG+YHLF+Q+NP GAV+   +VWAHS S DLINW 
Sbjct: 49  QPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWG-NIVWAHSTSTDLINWD 108

Query: 106 HLNPALEPTHPFDINGCWSGSASFLPGNKPVILYTGIDSSFNQVQNLAIPANFSDPFLEK 165
              PA+ P+ PFDINGCWSGSA+ LP  KPVILYTGID    QVQN+A P N SDP+L +
Sbjct: 109 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 168

Query: 166 WTKFPNNPIISPP--DHVLGDRFRDPTTAWRGPDGGWRVAIGGQTSDGGGAAMLYRSEDF 225
           W K P NP+++P   + +    FRDPTTAW G D  WRV IG +     G A+ Y S+DF
Sbjct: 169 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKI-HRRGLAITYTSKDF 228

Query: 226 IRWNLLRFPLYSSPDSGTWECPDFFPVLLNGTNGVDFSSGFGIG---VKHVMKASFN--S 285
           ++W     PL+    SG WECPDFFPV   G+NGV+ SS FG     +KHV+K S +   
Sbjct: 229 LKWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS-FGEPNEILKHVLKISLDDTK 288

Query: 286 HDYYTLGSYAPEEEKFTPDSGSDFGFRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWV 345
           HDYYT+G+Y   ++KF PD+    GF+      RYDYGK+YASKTF+DS++ RRILWGW 
Sbjct: 289 HDYYTIGTYDRVKDKFVPDN----GFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWT 348

Query: 346 NESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLIQWPVEEIKALRRNHF-SLNHKELIGR 405
           NES S EDD++KGWSG+Q +PRKIWL ++G+QLIQWPV E++ LR     +L +K L   
Sbjct: 349 NESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSG 408

Query: 406 STMKVPGGSASQVDAEVSFEFPYLEEAERMDTRWVDPQLLCSERNASVNGRVGPFGLLVL 465
           S ++V G +A+Q D EV F+   LE+A+ ++  W DPQL+CS+ N SV   +GPFGL+VL
Sbjct: 409 SRLEVYGVTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVL 468

Query: 466 ASNDLSEQTAVFFHILKAHK---RYVVLMCSDQSRSSFRNRVDKTTYGSFVDIDPRHEKI 525
           AS +L E T+V+F I KA +   +YVVLMCSDQSRSS +   DKTTYG+FVDI+P H+ +
Sbjct: 469 ASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPL 528

Query: 526 SLRTLVDHSVVESFAGEGKTCITSRVYPTLAVDKDARLYAFNNGTQSVVISSLNVWNMSK 576
           SLR L+DHSVVESF G+G+ CITSRVYP LA+ K + L+AFN G QSV + +LN W+M+ 
Sbjct: 529 SLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNS 584