MC08g0471 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0471
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionEndoglucanase
LocationMC08: 3835282 .. 3838111 (+)
RNA-Seq ExpressionMC08g0471
SyntenyMC08g0471
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTAACAAAACATAAAATTGCAATACTCATTAAAGTCCAAGATAAAAATTACCACATTGATAACAATCTAAATAATAGACCAACTTTATTATGTGTAAATTGAAATTTTCACAACCAAATTCATTTTTTAGCCTGTACTTAAATAGACTCCAGAGGAGAATGAATTTCACCATATTTTTAATTAGCAACCAAAAGATTTAAACACAGCCAAAAAGAAAAGAAATGACCAACCTAAAACAGTGGGGCGGCCAGAGTTTTCTCCAATCCCCACATCGTCTTCTCCGAAAATAACCTTAGAATCTGGAACGTCCCATGTTGTCCCCTTTCCAACCAACTTTAACTTCTCCGCCATTCTCAGACCTCAACTCTCCATTAATCTTCAACCGCAAACCTTCCAATTTTGAATCACCCTTCTCCGAACCGCATATACCCAACACCCCAATAACTCCAATTCAATTTCCCTTCCCCCTTTTATTCCCCCCATTCCCCTCATTCCTTCACCACCAATCCACCATCCCTCTTGATCTCGATCTCGATCTTCGCCATGGGAGCCTTGAAGATGAAGCTTTCTACCACCGCCATTGCTGTGGGTTTTCTAGCTCTGTTCCAAACTTTTCTGGTTGATGCCACTGAATTGAATTACAAAGATGCCCTCAGCAAGGCCCTCATTTTCCTCGAAGCTCAGAGGTCCGGCAAGCTCCCTCCCAATCACCGCCCCGCTTGGAGAGGCGACTCCGCCGTCGACGACGGCAAACTCGCCAATGTCTGCTCATTCTTCCATTTTCTCCGCTCTCTCTGTCAACTCAAATTTCTGATTTTAACACGCTTTGAATTTGCAGGTTGACCTTGAGGGAGGGTATTACGACGCCGGCGACAATGTGAAGTACGGACTTCCGATGGCTTTCACGGTTACAACTCTGTCATGGGCGGCCATGACTTACCAAGCGGAGATCGAAGCCGCCGGCGAAATGGAAAATGTTCGAGCCGCAATCAAATGGGGCACCGATTATTTCCTCAAGGCCTGCTCTCGCCGCAATCGTCTCTATGTTCAGGTAACAGGGACATCCCCTGTTTTTGCCCTGACATTGGTTTGAAATGAACAAAACATTGATGAAATTAAATTATTTATTTGATGGTGAAGGTGGGAGACCCGGTGAAGGATCATGAGTGTTGGGTTAGGCCTGAGAATATGAAGACGCCAAGAACTGTGCTGCAGATTGATAACAACACGCCCGGAACCGAAATTGCAGCTGAAACCTCCGCGGCCATGGCGTCTTCTTCCATTGTCTTCAGAAGCGTCAATAAAACCTATGCTCGTAGACTCCTAAACAAAGCAAAATTGGTACTAATTTCATTACTTCACACAAAATTTGCAAGTTCTGCCCTCTGGGATCAGGTAATTTTAACTAATTGGTAATCTTGTTCCCCCCTTCTCTGTTGCAGCTTTTTCAGTTCGCTAAAGCGCACAAAGGAACTTATGATGGAGAGTGCCCCTTCTATTGCTCTTTTTCAGGCTACAATGTATGTAACTCTCTAGTTATACGTCTGTTTTGCACGGCTTTATTTTGATTTCATAATAGCCCTGGTAGAACGAAGATAATTATACTCATCCATATAATATGAATCATGCTCGAACATGAGATTTTGTTTGGATTCAATTTACAAACCCGACACACAGGACGAGTTGTTGTGGGCAGCGACATGGCTATACATCGCAACCAAGAGGCCAGTGTATCTGAAGTACGTGAAAGAAGAAGCCATTACTGGGAGTGTAGCGGAATTCAGCTGGGACCTCAAATATGCAGGCGCCCAAATTCTTCTTTCCAAGGTAAATTTCATCAAATTACAACAACACCGTTTCAAATTCTGAAAAGGAATTGTGGAATTGCAGTTCTATTTCGAGGGAGAGAAGGGATTACAGACGTTGAAGGATCAGGCGGATAGTTACATATGCTCCAATCTTCCAAACAGCCCTTACCACCAAATCTACGTATCCCCAGGAGGAATGATTCACATGAGAGATGGCGCCAACACCCAATACGTTACAGGAACGGCTTTCTTGTTCAGTGCTTACAGCGACATTCTTGCAACCTTTAAACAGACCGTCAAGTGCGCCGACCAGCAGTTCGATTCCACCCAGCTCATGTCTTTCGCTAAGAAACAGGTAATCAAACCCACAAAATCCACCGCCTTAATTAGCGATTAATTGTGTTTGAGATTTCTAATCGGAAACGGGTGCGCGCAGATGGATTATGTGCTGGGAATCAACCCGCTGGGGAGATCTTACATGGTGGGGTTCGGAAACAACCCGCCGGTTCAGGCGCACCACCGCGGCGCCTCCGTGCCGGTTCTGGCGCCGAACGCGGAAGTGAGCTGCCCGATGAGTTTCGTGGAGTGGTTCAACAAGAACACCCCCAACCCCAACGAGCTCACCGGCGCCATTCTCGGCGGCCCCGATAAGAACGACAACTTCGTCGATAAGCGTTGGGATTCGCCGATGACGGAGCCCGTCACTTACACCAACTCCCTCGCCGTCGGAGTCATCGCCAAGCTCGCCGCCCACAAATTTACCTAATCGGAGCCCTAATTATCTAATTCTCTGCATCCTCTGGTTTTTTAGGCGTTTTTGAAATTAACAATTCTCTGCGATGTTCTTCTTCTTCTTCATCTTCTTCCTTCGTGGTCTTGCATTTTTTCGTTCTGTGCAGCCAAAGAAGACGATTGATTGATGCTTTCTGGGAAGTAGAGCCGTAACAGATTTTTCTGTTTGTCACTCATTGTAATATTTATCAATAATTAAAAAAAGGGCTAACTCTATTTTATATTTTCG

mRNA sequence

CCTAACAAAACATAAAATTGCAATACTCATTAAAGTCCAAGATAAAAATTACCACATTGATAACAATCTAAATAATAGACCAACTTTATTATGTGTAAATTGAAATTTTCACAACCAAATTCATTTTTTAGCCTGTACTTAAATAGACTCCAGAGGAGAATGAATTTCACCATATTTTTAATTAGCAACCAAAAGATTTAAACACAGCCAAAAAGAAAAGAAATGACCAACCTAAAACAGTGGGGCGGCCAGAGTTTTCTCCAATCCCCACATCGTCTTCTCCGAAAATAACCTTAGAATCTGGAACGTCCCATGTTGTCCCCTTTCCAACCAACTTTAACTTCTCCGCCATTCTCAGACCTCAACTCTCCATTAATCTTCAACCGCAAACCTTCCAATTTTGAATCACCCTTCTCCGAACCGCATATACCCAACACCCCAATAACTCCAATTCAATTTCCCTTCCCCCTTTTATTCCCCCCATTCCCCTCATTCCTTCACCACCAATCCACCATCCCTCTTGATCTCGATCTCGATCTTCGCCATGGGAGCCTTGAAGATGAAGCTTTCTACCACCGCCATTGCTGTGGGTTTTCTAGCTCTGTTCCAAACTTTTCTGGTTGATGCCACTGAATTGAATTACAAAGATGCCCTCAGCAAGGCCCTCATTTTCCTCGAAGCTCAGAGGTCCGGCAAGCTCCCTCCCAATCACCGCCCCGCTTGGAGAGGCGACTCCGCCGTCGACGACGGCAAACTCGCCAATGTTGACCTTGAGGGAGGGTATTACGACGCCGGCGACAATGTGAAGTACGGACTTCCGATGGCTTTCACGGTTACAACTCTGTCATGGGCGGCCATGACTTACCAAGCGGAGATCGAAGCCGCCGGCGAAATGGAAAATGTTCGAGCCGCAATCAAATGGGGCACCGATTATTTCCTCAAGGCCTGCTCTCGCCGCAATCGTCTCTATGTTCAGGTGGGAGACCCGGTGAAGGATCATGAGTGTTGGGTTAGGCCTGAGAATATGAAGACGCCAAGAACTGTGCTGCAGATTGATAACAACACGCCCGGAACCGAAATTGCAGCTGAAACCTCCGCGGCCATGGCGTCTTCTTCCATTGTCTTCAGAAGCGTCAATAAAACCTATGCTCGTAGACTCCTAAACAAAGCAAAATTGCTTTTTCAGTTCGCTAAAGCGCACAAAGGAACTTATGATGGAGAGTGCCCCTTCTATTGCTCTTTTTCAGGCTACAATGACGAGTTGTTGTGGGCAGCGACATGGCTATACATCGCAACCAAGAGGCCAGTGTATCTGAAGTACGTGAAAGAAGAAGCCATTACTGGGAGTGTAGCGGAATTCAGCTGGGACCTCAAATATGCAGGCGCCCAAATTCTTCTTTCCAAGTTCTATTTCGAGGGAGAGAAGGGATTACAGACGTTGAAGGATCAGGCGGATAGTTACATATGCTCCAATCTTCCAAACAGCCCTTACCACCAAATCTACGTATCCCCAGGAGGAATGATTCACATGAGAGATGGCGCCAACACCCAATACGTTACAGGAACGGCTTTCTTGTTCAGTGCTTACAGCGACATTCTTGCAACCTTTAAACAGACCGTCAAGTGCGCCGACCAGCAGTTCGATTCCACCCAGCTCATGTCTTTCGCTAAGAAACAGATGGATTATGTGCTGGGAATCAACCCGCTGGGGAGATCTTACATGGTGGGGTTCGGAAACAACCCGCCGGTTCAGGCGCACCACCGCGGCGCCTCCGTGCCGGTTCTGGCGCCGAACGCGGAAGTGAGCTGCCCGATGAGTTTCGTGGAGTGGTTCAACAAGAACACCCCCAACCCCAACGAGCTCACCGGCGCCATTCTCGGCGGCCCCGATAAGAACGACAACTTCGTCGATAAGCGTTGGGATTCGCCGATGACGGAGCCCGTCACTTACACCAACTCCCTCGCCGTCGGAGTCATCGCCAAGCTCGCCGCCCACAAATTTACCTAATCGGAGCCCTAATTATCTAATTCTCTGCATCCTCTGGTTTTTTAGGCGTTTTTGAAATTAACAATTCTCTGCGATGTTCTTCTTCTTCTTCATCTTCTTCCTTCGTGGTCTTGCATTTTTTCGTTCTGTGCAGCCAAAGAAGACGATTGATTGATGCTTTCTGGGAAGTAGAGCCGTAACAGATTTTTCTGTTTGTCACTCATTGTAATATTTATCAATAATTAAAAAAAGGGCTAACTCTATTTTATATTTTCG

Coding sequence (CDS)

ATGGGAGCCTTGAAGATGAAGCTTTCTACCACCGCCATTGCTGTGGGTTTTCTAGCTCTGTTCCAAACTTTTCTGGTTGATGCCACTGAATTGAATTACAAAGATGCCCTCAGCAAGGCCCTCATTTTCCTCGAAGCTCAGAGGTCCGGCAAGCTCCCTCCCAATCACCGCCCCGCTTGGAGAGGCGACTCCGCCGTCGACGACGGCAAACTCGCCAATGTTGACCTTGAGGGAGGGTATTACGACGCCGGCGACAATGTGAAGTACGGACTTCCGATGGCTTTCACGGTTACAACTCTGTCATGGGCGGCCATGACTTACCAAGCGGAGATCGAAGCCGCCGGCGAAATGGAAAATGTTCGAGCCGCAATCAAATGGGGCACCGATTATTTCCTCAAGGCCTGCTCTCGCCGCAATCGTCTCTATGTTCAGGTGGGAGACCCGGTGAAGGATCATGAGTGTTGGGTTAGGCCTGAGAATATGAAGACGCCAAGAACTGTGCTGCAGATTGATAACAACACGCCCGGAACCGAAATTGCAGCTGAAACCTCCGCGGCCATGGCGTCTTCTTCCATTGTCTTCAGAAGCGTCAATAAAACCTATGCTCGTAGACTCCTAAACAAAGCAAAATTGCTTTTTCAGTTCGCTAAAGCGCACAAAGGAACTTATGATGGAGAGTGCCCCTTCTATTGCTCTTTTTCAGGCTACAATGACGAGTTGTTGTGGGCAGCGACATGGCTATACATCGCAACCAAGAGGCCAGTGTATCTGAAGTACGTGAAAGAAGAAGCCATTACTGGGAGTGTAGCGGAATTCAGCTGGGACCTCAAATATGCAGGCGCCCAAATTCTTCTTTCCAAGTTCTATTTCGAGGGAGAGAAGGGATTACAGACGTTGAAGGATCAGGCGGATAGTTACATATGCTCCAATCTTCCAAACAGCCCTTACCACCAAATCTACGTATCCCCAGGAGGAATGATTCACATGAGAGATGGCGCCAACACCCAATACGTTACAGGAACGGCTTTCTTGTTCAGTGCTTACAGCGACATTCTTGCAACCTTTAAACAGACCGTCAAGTGCGCCGACCAGCAGTTCGATTCCACCCAGCTCATGTCTTTCGCTAAGAAACAGATGGATTATGTGCTGGGAATCAACCCGCTGGGGAGATCTTACATGGTGGGGTTCGGAAACAACCCGCCGGTTCAGGCGCACCACCGCGGCGCCTCCGTGCCGGTTCTGGCGCCGAACGCGGAAGTGAGCTGCCCGATGAGTTTCGTGGAGTGGTTCAACAAGAACACCCCCAACCCCAACGAGCTCACCGGCGCCATTCTCGGCGGCCCCGATAAGAACGACAACTTCGTCGATAAGCGTTGGGATTCGCCGATGACGGAGCCCGTCACTTACACCAACTCCCTCGCCGTCGGAGTCATCGCCAAGCTCGCCGCCCACAAATTTACCTAA

Protein sequence

MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLAAHKFT
Homology
BLAST of MC08g0471 vs. ExPASy Swiss-Prot
Match: Q8VYG3 (Endoglucanase 16 OS=Arabidopsis thaliana OX=3702 GN=At3g43860 PE=2 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 1.1e-206
Identity = 337/472 (71.40%), Postives = 391/472 (82.84%), Query Frame = 0

Query: 12  AIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKL 71
           ++ +G   +     V+ T +NYKDAL+K+LIFLEAQRSGKLPPN+R  WRGDSA+DDGKL
Sbjct: 14  SMLLGLYGIINIVCVNGTFINYKDALTKSLIFLEAQRSGKLPPNNRVPWRGDSALDDGKL 73

Query: 72  ANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYF 131
            NVDL GGYYDAGDNVKYGLPMAFT+TTL+W+ +TY+ E+ A GE+EN RAAI+WGTDYF
Sbjct: 74  VNVDLSGGYYDAGDNVKYGLPMAFTITTLAWSTITYEKELRATGELENARAAIRWGTDYF 133

Query: 132 LKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSS 191
           LK  SR+NRLYVQVGDP  DH+CW RPENMKTPRTVL+I +  PGTEIAAE +AA A+SS
Sbjct: 134 LKCASRKNRLYVQVGDPNADHQCWARPENMKTPRTVLEISDKVPGTEIAAEAAAAFAASS 193

Query: 192 IVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIAT 251
           IVFR V+  YARRLLNKAKLLF+ AK+HKGTYDGECPFYCS SGYNDEL+WAATWLY AT
Sbjct: 194 IVFRHVDHKYARRLLNKAKLLFKLAKSHKGTYDGECPFYCSNSGYNDELIWAATWLYKAT 253

Query: 252 KRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNL 311
           +  +YL Y+K EAI+  VAEFSWDLKYAGAQIL++K  FEG KGL   K QADS++CSNL
Sbjct: 254 RNHLYLSYLKFEAISAYVAEFSWDLKYAGAQILITKLIFEGHKGLDLYKQQADSFVCSNL 313

Query: 312 PNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQL 371
           P SPYHQ++ +PGGMIH+RDGAN+QYVT TAFLFSAY+DIL    Q + C   QFDST L
Sbjct: 314 PGSPYHQVFTTPGGMIHLRDGANSQYVTATAFLFSAYADILQKHNQKISCGSHQFDSTHL 373

Query: 372 MSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMSFVEWFN 431
           M+FAKKQ+DY+LG NP GRSYMVGFG NPP QAHHRGASVP+   NA +SCP+SFV+W+N
Sbjct: 374 MAFAKKQIDYILGHNPQGRSYMVGFGPNPPKQAHHRGASVPMHEANAPLSCPLSFVKWYN 433

Query: 432 KNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLAA 484
           KN PN NELTGAILGGPD+ D F D RW S  TEP TY NS+AVGV+AKLAA
Sbjct: 434 KNVPNANELTGAILGGPDRQDKFQDLRWTSVYTEPCTYINSIAVGVLAKLAA 485

BLAST of MC08g0471 vs. ExPASy Swiss-Prot
Match: Q6K7G9 (Endoglucanase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0778600 PE=2 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 2.6e-187
Identity = 305/455 (67.03%), Postives = 372/455 (81.76%), Query Frame = 0

Query: 28  ATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNV 87
           A   NYKDAL+K+++FLEAQRSGKLPP +R  WRGDS ++DGKLANVDL GGYYDAGDNV
Sbjct: 38  APSFNYKDALTKSIMFLEAQRSGKLPPTNRIKWRGDSGMEDGKLANVDLTGGYYDAGDNV 97

Query: 88  KYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGD 147
           KYGLP+AFTVTTL+W AM ++ E++AA E+ENV AAI+WGTDYFLKA ++++ L+VQVGD
Sbjct: 98  KYGLPLAFTVTTLAWTAMAFEKELKAARELENVHAAIRWGTDYFLKAATKKDHLWVQVGD 157

Query: 148 PVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLN 207
           P  DH+CWVRPENM TPRT+ QI++ TPG+EIAAET+AAM +SS+VFR  +K Y+RRLLN
Sbjct: 158 PNADHQCWVRPENMPTPRTLYQINDKTPGSEIAAETAAAMTASSMVFRK-DKPYSRRLLN 217

Query: 208 KAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAITG 267
           KAKLLFQFAK H+GTYDGECPFYCS+SGYNDELLWAATWLY+ATKR VY  ++  EAI+ 
Sbjct: 218 KAKLLFQFAKTHQGTYDGECPFYCSYSGYNDELLWAATWLYLATKRQVYADFIGHEAISS 277

Query: 268 SVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMI 327
           SVAEFSWDLK+ GAQ+LL++       GLQ+ K QAD+++C+ LP++P+HQ+ ++PGGMI
Sbjct: 278 SVAEFSWDLKFPGAQVLLAELNMTSSGGLQSFKSQADNFVCAVLPDTPFHQVSITPGGMI 337

Query: 328 HMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINP 387
           H+RDGAN+QYVT TAFLF AYSDIL    Q V C  Q     +L+ FAK+Q+DY+LG NP
Sbjct: 338 HLRDGANSQYVTSTAFLFVAYSDILRRINQPVMCGAQAVQPARLLQFAKQQIDYLLGANP 397

Query: 388 LGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGG 447
            GRSY+VGFG NPP Q HHRGAS PVL P  +V+C MSF EWF  + PNPNELTGAI+GG
Sbjct: 398 RGRSYVVGFGVNPPTQPHHRGASTPVLPPGYQVNCGMSFSEWFTPDRPNPNELTGAIMGG 457

Query: 448 PDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 483
           PD  DNF DKR +S  TEP TY NSL++G +A LA
Sbjct: 458 PDGGDNFSDKRGNSSCTEPCTYINSLSIGPLAALA 491

BLAST of MC08g0471 vs. ExPASy Swiss-Prot
Match: P22503 (Endoglucanase OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 461.5 bits (1186), Expect = 1.2e-128
Identity = 221/455 (48.57%), Postives = 307/455 (67.47%), Query Frame = 0

Query: 32  NYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGL 91
           +Y DAL+KA++F E QRSGKLP + R  WR DSA+ DGKL NV+L GGYYDAGDNVK+G 
Sbjct: 40  DYADALAKAILFFEGQRSGKLPSSQRVKWREDSALSDGKLQNVNLMGGYYDAGDNVKFGW 99

Query: 92  PMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKD 151
           PMAF+ + LSWAA+ Y++EI +  ++  +++AI+WG D+ L+A +    LY QVGD   D
Sbjct: 100 PMAFSTSLLSWAAVEYESEISSVNQLGYLQSAIRWGADFMLRAHTSPTTLYTQVGDGNAD 159

Query: 152 HECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKL 211
           H CW RPE+M TPRTV +ID N+PGTE+AAE +AA++++SIVF+ ++  Y+  LL+ +K 
Sbjct: 160 HNCWERPEDMDTPRTVYKIDANSPGTEVAAEYAAALSAASIVFKKIDAKYSSTLLSHSKS 219

Query: 212 LFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKY-VKEEAITGSVA 271
           LF FA  ++G+Y G CPFYCS+SGY DELLWAA WLY A+    YL Y +  +  + +V+
Sbjct: 220 LFDFADKNRGSYSGSCPFYCSYSGYQDELLWAAAWLYKASGESKYLSYIISNQGWSQTVS 279

Query: 272 EFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMR 331
           EFSWD K+ GAQ LL++ ++ G+K L  +K  A+S+IC+ +P S   QI  +PGG++  R
Sbjct: 280 EFSWDNKFVGAQTLLTEEFYGGKKDLAKIKTDAESFICAVMPGSNSRQIKTTPGGLLFTR 339

Query: 332 DGANTQYVTGTAFLFSAYSDIL-ATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLG 391
           D +N QY T +  +   +S IL       + C    F ++Q+  FAK Q++Y+LG NP+ 
Sbjct: 340 DSSNLQYTTSSTMVLFIFSRILNRNHINGINCGSSHFTASQIRGFAKTQVEYILGKNPMK 399

Query: 392 RSYMVGFGNNPPVQAHHRGASVPVLAPN-AEVSCPMSFVEWFNKNTPNPNELTGAILGGP 451
            SYMVGFG+  P Q HHRG+S+P +  + A+V C     +++N   PNPN   GAI+GGP
Sbjct: 400 MSYMVGFGSKYPKQLHHRGSSIPSIKVHPAKVGCNAGLSDYYNSANPNPNTHVGAIVGGP 459

Query: 452 DKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLAA 484
           D ND F D R D    EP TY N+  V  I+ L A
Sbjct: 460 DSNDRFNDARSDYSHAEPTTYINAAFVASISALLA 494

BLAST of MC08g0471 vs. ExPASy Swiss-Prot
Match: Q9SZ90 (Endoglucanase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g09740 PE=3 SV=2)

HSP 1 Score: 455.7 bits (1171), Expect = 6.7e-127
Identity = 222/454 (48.90%), Postives = 301/454 (66.30%), Query Frame = 0

Query: 31  LNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYG 90
           L+Y DAL+K+++F E QRSGKLP N R  WR DS + DG  ANV+L GGYYDAGDNVK+ 
Sbjct: 22  LDYGDALNKSILFFEGQRSGKLPTNQRVKWRADSGLSDGASANVNLIGGYYDAGDNVKFV 81

Query: 91  LPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVK 150
            PM+FT T LSWAA+ YQ EI    ++  +R+ IKWGT++ L+A +  N LY QVGD   
Sbjct: 82  WPMSFTTTLLSWAALEYQNEITFVNQLGYLRSTIKWGTNFILRAHTSTNMLYTQVGDGNS 141

Query: 151 DHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAK 210
           DH CW RPE+M TPRT+  I +++PG+E A E +AA+A++S+VF+ V+ TY+ +LLN AK
Sbjct: 142 DHSCWERPEDMDTPRTLYSISSSSPGSEAAGEAAAALAAASLVFKLVDSTYSSKLLNNAK 201

Query: 211 LLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYV-KEEAITGSV 270
            LF+FA  ++G+Y   CPFYCS SGY DELLWAA WLY AT    YL YV   +  + ++
Sbjct: 202 SLFEFADKYRGSYQASCPFYCSHSGYQDELLWAAAWLYKATGEKSYLNYVISNKDWSKAI 261

Query: 271 AEFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHM 330
            EFSWD K+AG Q LL+  ++ G   L+  K   +S++C+ +P S   QI  +PGG++ +
Sbjct: 262 NEFSWDNKFAGVQALLASEFYNGANDLEKFKTDVESFVCALMPGSSSQQIKPTPGGILFI 321

Query: 331 RDGANTQYV-TGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPL 390
           RD +N QYV T T  LF     +      +++C   QF  +Q+ +FAK Q+DY+LG NPL
Sbjct: 322 RDSSNLQYVTTATTILFYYSKTLTKAGVGSIQCGSTQFTVSQIRNFAKSQVDYILGNNPL 381

Query: 391 GRSYMVGFGNNPPVQAHHRGASVPVLAPNAE-VSCPMSFVEWFNKNTPNPNELTGAILGG 450
             SYMVGFG   P Q HHRG+S+P +    E + C   F  ++N +TPNPN  TGAI+GG
Sbjct: 382 KMSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGGF-SYYNFDTPNPNVHTGAIVGG 441

Query: 451 PDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 482
           P+ +D + DKR D    EP TY N+  +G +A L
Sbjct: 442 PNSSDQYSDKRTDYSHAEPTTYINAAFIGSVAAL 474

BLAST of MC08g0471 vs. ExPASy Swiss-Prot
Match: Q9SUS0 (Endoglucanase 20 OS=Arabidopsis thaliana OX=3702 GN=At4g23560 PE=2 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 8.7e-127
Identity = 220/454 (48.46%), Postives = 301/454 (66.30%), Query Frame = 0

Query: 31  LNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYG 90
           L Y DAL+K+++F E QRSGKLP N R  WR DSA+ DG LANV+L GGYYDAGDNVK+ 
Sbjct: 22  LEYGDALNKSILFFEGQRSGKLPTNQRVKWRADSALSDGSLANVNLIGGYYDAGDNVKFV 81

Query: 91  LPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVK 150
            PM+FT T LSWAA+ YQ EI +  ++  +R+ IKWGTD+ L+A +  N LY QVGD   
Sbjct: 82  WPMSFTTTLLSWAAIEYQNEISSVNQLGYLRSTIKWGTDFILRAHTSPNMLYTQVGDGNS 141

Query: 151 DHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAK 210
           DH CW RPE+M T RT+  I +++PG+E A E +AA+A++S+VF+SV+ TY+  LLN AK
Sbjct: 142 DHSCWERPEDMDTSRTLYSISSSSPGSEAAGEAAAALAAASLVFKSVDSTYSSTLLNHAK 201

Query: 211 LLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYV-KEEAITGSV 270
            LF+FA  ++G+Y   CPFYCS+SGY DELLWAA WLY AT   +Y+ YV   +  + +V
Sbjct: 202 TLFEFADKYRGSYQASCPFYCSYSGYQDELLWAAAWLYKATGDKIYINYVISNKDWSQAV 261

Query: 271 AEFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHM 330
            EFSWD K+ GAQ LL   ++ G   L   K   +S++C+ +P S   QI  +PGG++ +
Sbjct: 262 NEFSWDNKFVGAQALLVSEFYNGANDLAKFKSDVESFVCAMMPGSSSQQIKPTPGGLLFI 321

Query: 331 RDGANTQYV-TGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPL 390
           RD +N QYV T T  LF     +      +++C   +F  +Q+ +FAK Q+DY+LG NP+
Sbjct: 322 RDSSNLQYVTTATTVLFHYSKTLTKAGVGSIQCGSTKFTVSQIRNFAKSQVDYILGNNPM 381

Query: 391 GRSYMVGFGNNPPVQAHHRGASVPVLAPNAE-VSCPMSFVEWFNKNTPNPNELTGAILGG 450
             SYMVGFG   P Q HHRG+S+P +    E + C   +  ++N +TPNPN   GAI+GG
Sbjct: 382 KMSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGGY-SYYNSDTPNPNVHIGAIVGG 441

Query: 451 PDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 482
           P+ +D + DK+ D    EP TY N+  +G +A L
Sbjct: 442 PNSSDQYSDKKSDYSHAEPTTYINAAFIGPVAAL 474

BLAST of MC08g0471 vs. NCBI nr
Match: XP_022131779.1 (endoglucanase 16 [Momordica charantia])

HSP 1 Score: 991 bits (2562), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW 60
           MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW
Sbjct: 1   MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW 60

Query: 61  RGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV 120
           RGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV
Sbjct: 61  RGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV 120

Query: 121 RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 180
           RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA
Sbjct: 121 RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 180

Query: 181 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 240
           AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL
Sbjct: 181 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 240

Query: 241 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLK 300
           LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLK
Sbjct: 241 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLK 300

Query: 301 DQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVK 360
           DQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVK
Sbjct: 301 DQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVK 360

Query: 361 CADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEV 420
           CADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEV
Sbjct: 361 CADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEV 420

Query: 421 SCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAK 480
           SCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAK
Sbjct: 421 SCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAK 480

Query: 481 LAAHKFT 487
           LAAHKFT
Sbjct: 481 LAAHKFT 487

BLAST of MC08g0471 vs. NCBI nr
Match: XP_038886295.1 (endoglucanase 16 [Benincasa hispida])

HSP 1 Score: 872 bits (2253), Expect = 0.0
Identity = 422/491 (85.95%), Postives = 461/491 (93.89%), Query Frame = 0

Query: 1   MGALKMKLSTTAIAV-GFLALFQTFL----VDATELNYKDALSKALIFLEAQRSGKLPPN 60
           MGA+K KLSTT I +  ++  FQ  +    V+A +LNYKDAL+K+LIFLEAQRSGKLPPN
Sbjct: 1   MGAMKNKLSTTPIILLAWILFFQAIIFLGAVNA-DLNYKDALTKSLIFLEAQRSGKLPPN 60

Query: 61  HRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAG 120
           HRPAWRGDSA+DDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTL+WAA+TY AE+EAAG
Sbjct: 61  HRPAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLAWAALTYPAELEAAG 120

Query: 121 EMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTP 180
           EMEN +AAI+WGTDYFLKA SRRNRLYV+VGDPVKDHECWVRPENM+TPRTVLQID+NTP
Sbjct: 121 EMENTKAAIQWGTDYFLKAASRRNRLYVEVGDPVKDHECWVRPENMRTPRTVLQIDSNTP 180

Query: 181 GTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSG 240
           GTEIAAETSAAMASSS+VFRS N+TYARRLLNKAKLLFQFAK HKGTYDGECPFYCS+SG
Sbjct: 181 GTEIAAETSAAMASSSMVFRSCNRTYARRLLNKAKLLFQFAKNHKGTYDGECPFYCSYSG 240

Query: 241 YNDELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKG 300
           +NDELLWAATWLYIAT++PVYLKY++EE+I+ SVAEFSWDLKYAGAQILLSKFYFEGEK 
Sbjct: 241 FNDELLWAATWLYIATRKPVYLKYIQEESISASVAEFSWDLKYAGAQILLSKFYFEGEKE 300

Query: 301 LQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATF 360
           LQ  K+QADSYICSNLPNSPYHQIY+SPGGM+HMRDGANTQYVTGTAFLFSAYSD+LAT+
Sbjct: 301 LQMYKNQADSYICSNLPNSPYHQIYISPGGMVHMRDGANTQYVTGTAFLFSAYSDLLATY 360

Query: 361 KQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLA 420
           KQTV+CA  QFDSTQLM+FAKKQMDYVLGINPLGRSYMVGFGNNPP QAHHRGASVPVLA
Sbjct: 361 KQTVQCATVQFDSTQLMAFAKKQMDYVLGINPLGRSYMVGFGNNPPTQAHHRGASVPVLA 420

Query: 421 PNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAV 480
           P+  VSCPMSFV+WFNKNTPNPNELTGAILGGPD+ DNFVDKRWDSPMTEPVTYTNSLAV
Sbjct: 421 PDVVVSCPMSFVDWFNKNTPNPNELTGAILGGPDRTDNFVDKRWDSPMTEPVTYTNSLAV 480

Query: 481 GVIAKLAAHKF 486
           GV+AKLAAHKF
Sbjct: 481 GVLAKLAAHKF 490

BLAST of MC08g0471 vs. NCBI nr
Match: XP_023002656.1 (endoglucanase 16-like [Cucurbita maxima])

HSP 1 Score: 839 bits (2167), Expect = 1.62e-304
Identity = 405/490 (82.65%), Postives = 443/490 (90.41%), Query Frame = 0

Query: 1   MGALKMKLSTTAIAVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 60
           MG +   LS  AI VG L  FQ F    V A ELNYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAELNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 120
           PAWRGDSA+DDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY  E+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPTELEAAGEM 120

Query: 121 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 180
           ENV+AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQID+ TPGT
Sbjct: 121 ENVKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGT 180

Query: 181 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 240
           EIAAETSAAMASSSIVFRS N+TYAR LLNKAK L++FAK+HKGTYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSIVFRSSNQTYARLLLNKAKTLYKFAKSHKGTYDGECPFYCSYSGYN 240

Query: 241 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQ 300
           DELLWAATWLYIAT++ VYLKYV EE+I+ SVAEFSWDLKY GAQ+LLSK YFEGEKGL+
Sbjct: 241 DELLWAATWLYIATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSKLYFEGEKGLE 300

Query: 301 TLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQ 360
           T K+QAD+YICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA +KQ
Sbjct: 301 TFKNQADNYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQ 360

Query: 361 TVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN 420
            VKC+DQQFDST LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGAS+PV+  N
Sbjct: 361 NVKCSDQQFDSTHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASMPVMPAN 420

Query: 421 AEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGV 480
           AEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+AVGV
Sbjct: 421 AEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSMAVGV 480

Query: 481 IAKLAAHKFT 487
           +AKLAAHK T
Sbjct: 481 LAKLAAHKIT 490

BLAST of MC08g0471 vs. NCBI nr
Match: XP_023537900.1 (endoglucanase 16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832 bits (2150), Expect = 6.30e-302
Identity = 400/490 (81.63%), Postives = 441/490 (90.00%), Query Frame = 0

Query: 1   MGALKMKLSTTAIAVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 60
           MG +   LS  AI VG L  FQ F    V A E NYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAEFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 120
           PAWRGDSA+DDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY  E+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPTELEAAGEM 120

Query: 121 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 180
           EN++AAI+WGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQI++ TPGT
Sbjct: 121 ENLKAAIRWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQINSETPGT 180

Query: 181 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 240
           EIAAETSAAMASSS+VFRS N+TYAR LLNKAK L++FAKAHKGTYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSMVFRSSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFYCSYSGYN 240

Query: 241 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQ 300
           DELLWAATWLY+AT++ VYLKYV+EE+I+ SVAEFSWDLKY GAQ+LLSK YFEGEKGL+
Sbjct: 241 DELLWAATWLYVATRKSVYLKYVQEESISASVAEFSWDLKYVGAQVLLSKLYFEGEKGLE 300

Query: 301 TLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQ 360
           T K+QADSYICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA +KQ
Sbjct: 301 TFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQ 360

Query: 361 TVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN 420
            VKC+DQQFD   LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGASVPV+  N
Sbjct: 361 NVKCSDQQFDPAHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASVPVMPAN 420

Query: 421 AEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGV 480
           AEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+AVGV
Sbjct: 421 AEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSMAVGV 480

Query: 481 IAKLAAHKFT 487
           +AKLAAHK T
Sbjct: 481 LAKLAAHKIT 490

BLAST of MC08g0471 vs. NCBI nr
Match: XP_022951947.1 (endoglucanase 16-like [Cucurbita moschata])

HSP 1 Score: 832 bits (2148), Expect = 1.27e-301
Identity = 401/490 (81.84%), Postives = 439/490 (89.59%), Query Frame = 0

Query: 1   MGALKMKLSTTAIAVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 60
           MG +   LS  AI VG L  FQ F    V A + NYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 120
           PAWRGDSA+DDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY AE+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEM 120

Query: 121 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 180
           EN++AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQID+ TPGT
Sbjct: 121 ENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGT 180

Query: 181 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 240
           EIAAETSAAMASSSIVFR  N+TYAR LLNKAK L++FAKAHK TYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKATYDGECPFYCSYSGYN 240

Query: 241 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQ 300
           DELLWAATWLY+AT++ VYLKYV EE+I+ SVAEFSWDLKY GAQ+LLSK YFEGEKGL+
Sbjct: 241 DELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSKLYFEGEKGLE 300

Query: 301 TLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQ 360
           T K+QADSYICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA +KQ
Sbjct: 301 TFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQ 360

Query: 361 TVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN 420
            VKC+DQQFD   LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGASVPV+  N
Sbjct: 361 NVKCSDQQFDPAHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASVPVMPAN 420

Query: 421 AEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGV 480
           AEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+AVGV
Sbjct: 421 AEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSMAVGV 480

Query: 481 IAKLAAHKFT 487
           +AKLAAHK T
Sbjct: 481 LAKLAAHKIT 490

BLAST of MC08g0471 vs. ExPASy TrEMBL
Match: A0A6J1BR89 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111004858 PE=3 SV=1)

HSP 1 Score: 991 bits (2562), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW 60
           MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW
Sbjct: 1   MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW 60

Query: 61  RGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV 120
           RGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV
Sbjct: 61  RGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV 120

Query: 121 RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 180
           RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA
Sbjct: 121 RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 180

Query: 181 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 240
           AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL
Sbjct: 181 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 240

Query: 241 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLK 300
           LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLK
Sbjct: 241 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLK 300

Query: 301 DQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVK 360
           DQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVK
Sbjct: 301 DQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVK 360

Query: 361 CADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEV 420
           CADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEV
Sbjct: 361 CADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEV 420

Query: 421 SCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAK 480
           SCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAK
Sbjct: 421 SCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAK 480

Query: 481 LAAHKFT 487
           LAAHKFT
Sbjct: 481 LAAHKFT 487

BLAST of MC08g0471 vs. ExPASy TrEMBL
Match: A0A6J1KLX1 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111496447 PE=3 SV=1)

HSP 1 Score: 839 bits (2167), Expect = 7.85e-305
Identity = 405/490 (82.65%), Postives = 443/490 (90.41%), Query Frame = 0

Query: 1   MGALKMKLSTTAIAVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 60
           MG +   LS  AI VG L  FQ F    V A ELNYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAELNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 120
           PAWRGDSA+DDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY  E+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPTELEAAGEM 120

Query: 121 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 180
           ENV+AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQID+ TPGT
Sbjct: 121 ENVKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGT 180

Query: 181 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 240
           EIAAETSAAMASSSIVFRS N+TYAR LLNKAK L++FAK+HKGTYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSIVFRSSNQTYARLLLNKAKTLYKFAKSHKGTYDGECPFYCSYSGYN 240

Query: 241 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQ 300
           DELLWAATWLYIAT++ VYLKYV EE+I+ SVAEFSWDLKY GAQ+LLSK YFEGEKGL+
Sbjct: 241 DELLWAATWLYIATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSKLYFEGEKGLE 300

Query: 301 TLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQ 360
           T K+QAD+YICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA +KQ
Sbjct: 301 TFKNQADNYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQ 360

Query: 361 TVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN 420
            VKC+DQQFDST LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGAS+PV+  N
Sbjct: 361 NVKCSDQQFDSTHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASMPVMPAN 420

Query: 421 AEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGV 480
           AEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+AVGV
Sbjct: 421 AEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSMAVGV 480

Query: 481 IAKLAAHKFT 487
           +AKLAAHK T
Sbjct: 481 LAKLAAHKIT 490

BLAST of MC08g0471 vs. ExPASy TrEMBL
Match: A0A6J1GJ20 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111454680 PE=3 SV=1)

HSP 1 Score: 832 bits (2148), Expect = 6.15e-302
Identity = 401/490 (81.84%), Postives = 439/490 (89.59%), Query Frame = 0

Query: 1   MGALKMKLSTTAIAVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 60
           MG +   LS  AI VG L  FQ F    V A + NYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 120
           PAWRGDSA+DDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY AE+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEM 120

Query: 121 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 180
           EN++AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQID+ TPGT
Sbjct: 121 ENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGT 180

Query: 181 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 240
           EIAAETSAAMASSSIVFR  N+TYAR LLNKAK L++FAKAHK TYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKATYDGECPFYCSYSGYN 240

Query: 241 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQ 300
           DELLWAATWLY+AT++ VYLKYV EE+I+ SVAEFSWDLKY GAQ+LLSK YFEGEKGL+
Sbjct: 241 DELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSKLYFEGEKGLE 300

Query: 301 TLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQ 360
           T K+QADSYICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA +KQ
Sbjct: 301 TFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQ 360

Query: 361 TVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN 420
            VKC+DQQFD   LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGASVPV+  N
Sbjct: 361 NVKCSDQQFDPAHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASVPVMPAN 420

Query: 421 AEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGV 480
           AEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+AVGV
Sbjct: 421 AEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSMAVGV 480

Query: 481 IAKLAAHKFT 487
           +AKLAAHK T
Sbjct: 481 LAKLAAHKIT 490

BLAST of MC08g0471 vs. ExPASy TrEMBL
Match: A0A1S3BDP2 (Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103488526 PE=3 SV=1)

HSP 1 Score: 830 bits (2143), Expect = 3.83e-301
Identity = 401/485 (82.68%), Postives = 442/485 (91.13%), Query Frame = 0

Query: 7   KLSTTAIA--VGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWR 66
            +STT I   V  L  FQ+ +   V   +LNYKDAL+K+LIFLEAQRSGKLPPNHRPAWR
Sbjct: 8   NVSTTPILLLVYCLVFFQSIVFLRVVNADLNYKDALTKSLIFLEAQRSGKLPPNHRPAWR 67

Query: 67  GDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVR 126
           GDSA+DDGKLANVDL GGYYDAGDNVKYGLPMAFT+TTL+W A+TY  EI+AAGEMENV+
Sbjct: 68  GDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTITTLAWGALTYPEEIKAAGEMENVK 127

Query: 127 AAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAA 186
           AA++WGTDYFLKA SRRNRLYVQVGDPVKDHECWVRPENMKT RTVLQID+NTPGTEIAA
Sbjct: 128 AALQWGTDYFLKAASRRNRLYVQVGDPVKDHECWVRPENMKTERTVLQIDSNTPGTEIAA 187

Query: 187 ETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELL 246
           ETSAAMAS+S+VFRS N+TYARRLLNKAKLL+Q AK HKGTYDGECPFYCS+SGYNDELL
Sbjct: 188 ETSAAMASASMVFRSSNRTYARRLLNKAKLLYQLAKNHKGTYDGECPFYCSYSGYNDELL 247

Query: 247 WAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLKD 306
           WAATWLYIAT+RPVYLKYV+EE+I+ SVAEFSWDLKYAGAQILLSKFYFEGEKGLQ  K+
Sbjct: 248 WAATWLYIATRRPVYLKYVQEESISASVAEFSWDLKYAGAQILLSKFYFEGEKGLQMYKN 307

Query: 307 QADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKC 366
           QADSYICSNLP SP+HQI++SPGGM+H+RDGANTQYV GTAFLFSAYSD+LAT+KQT +C
Sbjct: 308 QADSYICSNLPESPFHQIFISPGGMVHLRDGANTQYVAGTAFLFSAYSDLLATYKQTAQC 367

Query: 367 ADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVS 426
           AD+ FDS QLM FAKKQMDY+LGINPLGRS+MVGFGNNPP QAHHRGASVPVL PN  V+
Sbjct: 368 ADKSFDSKQLMMFAKKQMDYMLGINPLGRSFMVGFGNNPPKQAHHRGASVPVLPPNTVVN 427

Query: 427 CPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 486
           CPMSFV+WFNK+TPNPNELTGAILGGPD+ D F DKR DSPMTEPVTYTNSLAVGV+AKL
Sbjct: 428 CPMSFVDWFNKDTPNPNELTGAILGGPDRTDKFNDKRSDSPMTEPVTYTNSLAVGVLAKL 487

BLAST of MC08g0471 vs. ExPASy TrEMBL
Match: A0A5A7VBS6 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00080 PE=3 SV=1)

HSP 1 Score: 825 bits (2131), Expect = 2.67e-299
Identity = 401/486 (82.51%), Postives = 442/486 (90.95%), Query Frame = 0

Query: 7   KLSTTAIA--VGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWR 66
            +STT I   V  L  FQ+ +   V   +LNYKDAL+K+LIFLEAQRSGKLPPNHRPAWR
Sbjct: 8   NVSTTPILLLVYCLVFFQSIVFLRVVNADLNYKDALTKSLIFLEAQRSGKLPPNHRPAWR 67

Query: 67  GDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVR 126
           GDSA+DDGKLANVDL GGYYDAGDNVKYGLPMAFT+TTL+W A+TY  EI+AAGEMENV+
Sbjct: 68  GDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTITTLAWGALTYPEEIKAAGEMENVK 127

Query: 127 AAIKWGTDYFLKACSRRNRLYVQV-GDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 186
           AA++WGTDYFLKA SRRNRLYVQV GDPVKDHECWVRPENMKT RTVLQID+NTPGTEIA
Sbjct: 128 AALQWGTDYFLKAASRRNRLYVQVVGDPVKDHECWVRPENMKTERTVLQIDSNTPGTEIA 187

Query: 187 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 246
           AETSAAMAS+S+VFRS N+TYARRLLNKAKLL+Q AK HKGTYDGECPFYCS+SGYNDEL
Sbjct: 188 AETSAAMASASMVFRSSNRTYARRLLNKAKLLYQLAKNHKGTYDGECPFYCSYSGYNDEL 247

Query: 247 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLK 306
           LWAATWLYIAT+RPVYLKYV+EE+I+ SVAEFSWDLKYAGAQILLSKFYFEGEKGLQ  K
Sbjct: 248 LWAATWLYIATRRPVYLKYVQEESISASVAEFSWDLKYAGAQILLSKFYFEGEKGLQMYK 307

Query: 307 DQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVK 366
           +QADSYICSNLP SP+HQI++SPGGM+H+RDGANTQYV GTAFLFSAYSD+LAT+KQT +
Sbjct: 308 NQADSYICSNLPESPFHQIFISPGGMVHLRDGANTQYVAGTAFLFSAYSDLLATYKQTAQ 367

Query: 367 CADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEV 426
           CAD+ FDS QLM FAKKQMDY+LGINPLGRS+MVGFGNNPP QAHHRGASVPVL PN  V
Sbjct: 368 CADKSFDSKQLMMFAKKQMDYMLGINPLGRSFMVGFGNNPPKQAHHRGASVPVLPPNTVV 427

Query: 427 SCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAK 486
           +CPMSFV+WFNK+TPNPNELTGAILGGPD+ D F DKR DSPMTEPVTYTNSLAVGV+AK
Sbjct: 428 NCPMSFVDWFNKDTPNPNELTGAILGGPDRTDKFNDKRSDSPMTEPVTYTNSLAVGVLAK 487

BLAST of MC08g0471 vs. TAIR 10
Match: AT3G43860.1 (glycosyl hydrolase 9A4 )

HSP 1 Score: 720.7 bits (1859), Expect = 7.9e-208
Identity = 337/472 (71.40%), Postives = 391/472 (82.84%), Query Frame = 0

Query: 12  AIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKL 71
           ++ +G   +     V+ T +NYKDAL+K+LIFLEAQRSGKLPPN+R  WRGDSA+DDGKL
Sbjct: 14  SMLLGLYGIINIVCVNGTFINYKDALTKSLIFLEAQRSGKLPPNNRVPWRGDSALDDGKL 73

Query: 72  ANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYF 131
            NVDL GGYYDAGDNVKYGLPMAFT+TTL+W+ +TY+ E+ A GE+EN RAAI+WGTDYF
Sbjct: 74  VNVDLSGGYYDAGDNVKYGLPMAFTITTLAWSTITYEKELRATGELENARAAIRWGTDYF 133

Query: 132 LKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSS 191
           LK  SR+NRLYVQVGDP  DH+CW RPENMKTPRTVL+I +  PGTEIAAE +AA A+SS
Sbjct: 134 LKCASRKNRLYVQVGDPNADHQCWARPENMKTPRTVLEISDKVPGTEIAAEAAAAFAASS 193

Query: 192 IVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIAT 251
           IVFR V+  YARRLLNKAKLLF+ AK+HKGTYDGECPFYCS SGYNDEL+WAATWLY AT
Sbjct: 194 IVFRHVDHKYARRLLNKAKLLFKLAKSHKGTYDGECPFYCSNSGYNDELIWAATWLYKAT 253

Query: 252 KRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNL 311
           +  +YL Y+K EAI+  VAEFSWDLKYAGAQIL++K  FEG KGL   K QADS++CSNL
Sbjct: 254 RNHLYLSYLKFEAISAYVAEFSWDLKYAGAQILITKLIFEGHKGLDLYKQQADSFVCSNL 313

Query: 312 PNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQL 371
           P SPYHQ++ +PGGMIH+RDGAN+QYVT TAFLFSAY+DIL    Q + C   QFDST L
Sbjct: 314 PGSPYHQVFTTPGGMIHLRDGANSQYVTATAFLFSAYADILQKHNQKISCGSHQFDSTHL 373

Query: 372 MSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMSFVEWFN 431
           M+FAKKQ+DY+LG NP GRSYMVGFG NPP QAHHRGASVP+   NA +SCP+SFV+W+N
Sbjct: 374 MAFAKKQIDYILGHNPQGRSYMVGFGPNPPKQAHHRGASVPMHEANAPLSCPLSFVKWYN 433

Query: 432 KNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLAA 484
           KN PN NELTGAILGGPD+ D F D RW S  TEP TY NS+AVGV+AKLAA
Sbjct: 434 KNVPNANELTGAILGGPDRQDKFQDLRWTSVYTEPCTYINSIAVGVLAKLAA 485

BLAST of MC08g0471 vs. TAIR 10
Match: AT4G09740.1 (glycosyl hydrolase 9B14 )

HSP 1 Score: 455.7 bits (1171), Expect = 4.7e-128
Identity = 222/454 (48.90%), Postives = 301/454 (66.30%), Query Frame = 0

Query: 31  LNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYG 90
           L+Y DAL+K+++F E QRSGKLP N R  WR DS + DG  ANV+L GGYYDAGDNVK+ 
Sbjct: 22  LDYGDALNKSILFFEGQRSGKLPTNQRVKWRADSGLSDGASANVNLIGGYYDAGDNVKFV 81

Query: 91  LPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVK 150
            PM+FT T LSWAA+ YQ EI    ++  +R+ IKWGT++ L+A +  N LY QVGD   
Sbjct: 82  WPMSFTTTLLSWAALEYQNEITFVNQLGYLRSTIKWGTNFILRAHTSTNMLYTQVGDGNS 141

Query: 151 DHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAK 210
           DH CW RPE+M TPRT+  I +++PG+E A E +AA+A++S+VF+ V+ TY+ +LLN AK
Sbjct: 142 DHSCWERPEDMDTPRTLYSISSSSPGSEAAGEAAAALAAASLVFKLVDSTYSSKLLNNAK 201

Query: 211 LLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYV-KEEAITGSV 270
            LF+FA  ++G+Y   CPFYCS SGY DELLWAA WLY AT    YL YV   +  + ++
Sbjct: 202 SLFEFADKYRGSYQASCPFYCSHSGYQDELLWAAAWLYKATGEKSYLNYVISNKDWSKAI 261

Query: 271 AEFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHM 330
            EFSWD K+AG Q LL+  ++ G   L+  K   +S++C+ +P S   QI  +PGG++ +
Sbjct: 262 NEFSWDNKFAGVQALLASEFYNGANDLEKFKTDVESFVCALMPGSSSQQIKPTPGGILFI 321

Query: 331 RDGANTQYV-TGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPL 390
           RD +N QYV T T  LF     +      +++C   QF  +Q+ +FAK Q+DY+LG NPL
Sbjct: 322 RDSSNLQYVTTATTILFYYSKTLTKAGVGSIQCGSTQFTVSQIRNFAKSQVDYILGNNPL 381

Query: 391 GRSYMVGFGNNPPVQAHHRGASVPVLAPNAE-VSCPMSFVEWFNKNTPNPNELTGAILGG 450
             SYMVGFG   P Q HHRG+S+P +    E + C   F  ++N +TPNPN  TGAI+GG
Sbjct: 382 KMSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGGF-SYYNFDTPNPNVHTGAIVGG 441

Query: 451 PDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 482
           P+ +D + DKR D    EP TY N+  +G +A L
Sbjct: 442 PNSSDQYSDKRTDYSHAEPTTYINAAFIGSVAAL 474

BLAST of MC08g0471 vs. TAIR 10
Match: AT4G23560.1 (glycosyl hydrolase 9B15 )

HSP 1 Score: 455.3 bits (1170), Expect = 6.2e-128
Identity = 220/454 (48.46%), Postives = 301/454 (66.30%), Query Frame = 0

Query: 31  LNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYG 90
           L Y DAL+K+++F E QRSGKLP N R  WR DSA+ DG LANV+L GGYYDAGDNVK+ 
Sbjct: 22  LEYGDALNKSILFFEGQRSGKLPTNQRVKWRADSALSDGSLANVNLIGGYYDAGDNVKFV 81

Query: 91  LPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVK 150
            PM+FT T LSWAA+ YQ EI +  ++  +R+ IKWGTD+ L+A +  N LY QVGD   
Sbjct: 82  WPMSFTTTLLSWAAIEYQNEISSVNQLGYLRSTIKWGTDFILRAHTSPNMLYTQVGDGNS 141

Query: 151 DHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAK 210
           DH CW RPE+M T RT+  I +++PG+E A E +AA+A++S+VF+SV+ TY+  LLN AK
Sbjct: 142 DHSCWERPEDMDTSRTLYSISSSSPGSEAAGEAAAALAAASLVFKSVDSTYSSTLLNHAK 201

Query: 211 LLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYV-KEEAITGSV 270
            LF+FA  ++G+Y   CPFYCS+SGY DELLWAA WLY AT   +Y+ YV   +  + +V
Sbjct: 202 TLFEFADKYRGSYQASCPFYCSYSGYQDELLWAAAWLYKATGDKIYINYVISNKDWSQAV 261

Query: 271 AEFSWDLKYAGAQILLSKFYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHM 330
            EFSWD K+ GAQ LL   ++ G   L   K   +S++C+ +P S   QI  +PGG++ +
Sbjct: 262 NEFSWDNKFVGAQALLVSEFYNGANDLAKFKSDVESFVCAMMPGSSSQQIKPTPGGLLFI 321

Query: 331 RDGANTQYV-TGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPL 390
           RD +N QYV T T  LF     +      +++C   +F  +Q+ +FAK Q+DY+LG NP+
Sbjct: 322 RDSSNLQYVTTATTVLFHYSKTLTKAGVGSIQCGSTKFTVSQIRNFAKSQVDYILGNNPM 381

Query: 391 GRSYMVGFGNNPPVQAHHRGASVPVLAPNAE-VSCPMSFVEWFNKNTPNPNELTGAILGG 450
             SYMVGFG   P Q HHRG+S+P +    E + C   +  ++N +TPNPN   GAI+GG
Sbjct: 382 KMSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGGY-SYYNSDTPNPNVHIGAIVGG 441

Query: 451 PDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 482
           P+ +D + DK+ D    EP TY N+  +G +A L
Sbjct: 442 PNSSDQYSDKKSDYSHAEPTTYINAAFIGPVAAL 474

BLAST of MC08g0471 vs. TAIR 10
Match: AT4G02290.1 (glycosyl hydrolase 9B13 )

HSP 1 Score: 445.3 bits (1144), Expect = 6.4e-125
Identity = 223/462 (48.27%), Postives = 303/462 (65.58%), Query Frame = 0

Query: 32  NYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGL 91
           NYKDAL+K+++F E QRSGKLP N R +WR DS + DG   +VDL GGYYDAGDN+K+G 
Sbjct: 52  NYKDALTKSILFFEGQRSGKLPSNQRMSWRRDSGLSDGSALHVDLVGGYYDAGDNIKFGF 111

Query: 92  PMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKD 151
           PMAFT T LSW+ + +   +++  E++N + AI+W TDY LKA S+ + +YVQVGD  KD
Sbjct: 112 PMAFTTTMLSWSVIEFGGLMKS--ELQNAKIAIRWATDYLLKATSQPDTIYVQVGDANKD 171

Query: 152 HECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKL 211
           H CW RPE+M T R+V ++D N PG+++AAET+AA+A+++IVFR  + +Y++ LL +A  
Sbjct: 172 HSCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAAAAIVFRKSDPSYSKVLLKRAIS 231

Query: 212 LFQFAKAHKGTYDG-----ECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAIT 271
           +F FA  ++GTY        CPFYCS+SGY DELLW A WL  ATK   YL Y+K     
Sbjct: 232 VFAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGAAWLQKATKNIKYLNYIKINGQI 291

Query: 272 GSVAE----FSWDLKYAGAQILLSK-FYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYV 331
              AE    F WD K+AGA+ILL+K F  +  K L   K  AD++ICS +P +P+     
Sbjct: 292 LGAAEYDNTFGWDNKHAGARILLTKAFLVQNVKTLHEYKGHADNFICSVIPGAPFSSTQY 351

Query: 332 SPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDY 391
           +PGG++     AN QYVT T+FL   Y+  L + K  V C    +   +L S AK+Q+DY
Sbjct: 352 TPGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAKTVVHCGGSVYTPGRLRSIAKRQVDY 411

Query: 392 VLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN-AEVSCPMSFVEWFNKNTPNPNEL 451
           +LG NPL  SYMVG+G   P + HHRG+S+P +A + A++ C   F    N  +PNPN L
Sbjct: 412 LLGDNPLRMSYMVGYGPKFPRRIHHRGSSLPCVASHPAKIQCHQGFA-IMNSQSPNPNFL 471

Query: 452 TGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 483
            GA++GGPD++D F D+R D   +EP TY NS  VG +A  A
Sbjct: 472 VGAVVGGPDQHDRFPDERSDYEQSEPATYINSPLVGALAYFA 510

BLAST of MC08g0471 vs. TAIR 10
Match: AT1G02800.1 (cellulase 2 )

HSP 1 Score: 441.4 bits (1134), Expect = 9.3e-124
Identity = 224/462 (48.48%), Postives = 296/462 (64.07%), Query Frame = 0

Query: 32  NYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSAVDDGKLANVDLEGGYYDAGDNVKYGL 91
           NYKDALSK+++F E QRSGKLPPN R  WR +S + DG   NVDL GGYYDAGDN+K+G 
Sbjct: 43  NYKDALSKSILFFEGQRSGKLPPNQRMTWRSNSGLSDGSALNVDLVGGYYDAGDNMKFGF 102

Query: 92  PMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKD 151
           PMAFT T LSW+ + +   +++  E+ N + AI+W TD+ LKA S  + +YVQVGDP  D
Sbjct: 103 PMAFTTTMLSWSLIEFGGLMKS--ELPNAKDAIRWATDFLLKATSHPDTIYVQVGDPNMD 162

Query: 152 HECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKL 211
           H CW RPE+M TPR+V ++D N PG++IA E +AA+A++SIVFR  + +Y+  LL +A  
Sbjct: 163 HACWERPEDMDTPRSVFKVDKNNPGSDIAGEIAAALAAASIVFRKCDPSYSNHLLQRAIT 222

Query: 212 LFQFAKAHKGTYDG-----ECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAIT 271
           +F FA  ++G Y        CPFYCS+SGY DELLW A WL  AT  P YL Y+K     
Sbjct: 223 VFTFADKYRGPYSAGLAPEVCPFYCSYSGYQDELLWGAAWLQKATNNPTYLNYIKANGQI 282

Query: 272 GSVAE----FSWDLKYAGAQILLSK-FYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYV 331
               E    FSWD K+ GA+ILLSK F  +  K L+  K+ ADS+ICS LP +   Q   
Sbjct: 283 LGADEFDNMFSWDNKHVGARILLSKEFLIQKVKSLEEYKEHADSFICSVLPGASSSQ--Y 342

Query: 332 SPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDY 391
           +PGG++     +N QYVT T+FL   Y+  L + +    C        +L S AKKQ+DY
Sbjct: 343 TPGGLLFKMGESNMQYVTSTSFLLLTYAKYLTSARTVAYCGGSVVTPARLRSIAKKQVDY 402

Query: 392 VLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN-AEVSCPMSFVEWFNKNTPNPNEL 451
           +LG NPL  SYMVG+G   P + HHRG+S+P +A +   + C   F   F   +PNPN+L
Sbjct: 403 LLGGNPLKMSYMVGYGLKYPRRIHHRGSSLPSVAVHPTRIQCHDGF-SLFTSQSPNPNDL 462

Query: 452 TGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 483
            GA++GGPD+ND F D+R D   +EP TY N+  VG +A LA
Sbjct: 463 VGAVVGGPDQNDQFPDERSDYGRSEPATYINAPLVGALAYLA 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYG31.1e-20671.40Endoglucanase 16 OS=Arabidopsis thaliana OX=3702 GN=At3g43860 PE=2 SV=1[more]
Q6K7G92.6e-18767.03Endoglucanase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0778600 PE=2 SV... [more]
P225031.2e-12848.57Endoglucanase OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
Q9SZ906.7e-12748.90Endoglucanase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g09740 PE=3 SV=2[more]
Q9SUS08.7e-12748.46Endoglucanase 20 OS=Arabidopsis thaliana OX=3702 GN=At4g23560 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022131779.10.0100.00endoglucanase 16 [Momordica charantia][more]
XP_038886295.10.085.95endoglucanase 16 [Benincasa hispida][more]
XP_023002656.11.62e-30482.65endoglucanase 16-like [Cucurbita maxima][more]
XP_023537900.16.30e-30281.63endoglucanase 16-like [Cucurbita pepo subsp. pepo][more]
XP_022951947.11.27e-30181.84endoglucanase 16-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1BR890.0100.00Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111004858 PE=3 SV=1[more]
A0A6J1KLX17.85e-30582.65Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111496447 PE=3 SV=1[more]
A0A6J1GJ206.15e-30281.84Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111454680 PE=3 SV=1[more]
A0A1S3BDP23.83e-30182.68Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103488526 PE=3 SV=1[more]
A0A5A7VBS62.67e-29982.51Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00080 ... [more]
Match NameE-valueIdentityDescription
AT3G43860.17.9e-20871.40glycosyl hydrolase 9A4 [more]
AT4G09740.14.7e-12848.90glycosyl hydrolase 9B14 [more]
AT4G23560.16.2e-12848.46glycosyl hydrolase 9B15 [more]
AT4G02290.16.4e-12548.27glycosyl hydrolase 9B13 [more]
AT1G02800.19.3e-12448.48cellulase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 30..485
e-value: 1.2E-154
score: 517.6
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 33..477
e-value: 1.1E-136
score: 456.8
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 12..483
NoneNo IPR availablePANTHERPTHR22298:SF104ENDOGLUCANASE 16-RELATEDcoord: 12..483
IPR018221Glycoside hydrolase family 9, His active sitePROSITEPS00592GH9_2coord: 381..407
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 32..483

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0471.1MC08g0471.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030245 cellulose catabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008810 cellulase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds