Homology
BLAST of MC08g0468 vs. ExPASy Swiss-Prot
Match:
Q5N870 (Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica OX=39947 GN=DCL3A PE=2 SV=1)
HSP 1 Score: 1566.2 bits (4054), Expect = 0.0e+00
Identity = 844/1640 (51.46%), Postives = 1107/1640 (67.50%), Query Frame = 0
Query: 39 FIPRRYQTEVFEVGMRRNTIAVLETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTV 98
F PRRYQ +V+EV MRRNTIA+L+TGAGKTMIAVMLIKE G ++ + K IIFLAPTV
Sbjct: 30 FTPRRYQLDVYEVAMRRNTIAMLDTGAGKTMIAVMLIKEFGKINRTKNAGKVIIFLAPTV 89
Query: 99 HLVHQQFNVIKAHTDFEVAEYYGAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAF 158
LV QQ VI+ HTDFEV +YYGA GVD W+ W+++ S++ V+VMTPQ+ L ALR AF
Sbjct: 90 QLVTQQCEVIEIHTDFEVEQYYGAKGVDQWTGPRWQEQISKYQVMVMTPQVFLQALRNAF 149
Query: 159 LSLGTVCLMIIDECHRATGNHPYTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCE 218
L L V LMI DECH ATGNHPYT+IMK+FYHKS++KP +FGMTASPVIRKG+SS DCE
Sbjct: 150 LILDMVSLMIFDECHHATGNHPYTRIMKEFYHKSEHKPSVFGMTASPVIRKGISSHLDCE 209
Query: 219 CQIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDA 278
Q +LE+++D++IY + D++E+E VPSAKE+C +Y+ +L ++ L K+DA
Sbjct: 210 GQFCELENLLDAKIYTVSDREEIEFCVPSAKEMCRYYDSKPVCFEDLSEELGVLCSKYDA 269
Query: 279 LLSNSQGAVQVHYKDVDSKLKPLKKRLSSDHLKISYCLDELGLICAYEAIKVLLEN---- 338
L++ Q YKD D K K+RLS KI YCLD++GLICA EA K+ +E
Sbjct: 270 LITELQNKRSDMYKDADDITKESKRRLSKSIAKICYCLDDVGLICASEATKICIERGQEK 329
Query: 339 ------VNITNEEYE-----VFRESSLQYKCFLEEALLVIGESFPLGNENVLNFGLDIWK 398
V+ T+++ + +F E+S + F EEAL +I + G + +LN +
Sbjct: 330 GWLKEVVDATDQQTDANGSRLFAENSALHMKFFEEALHLIDKRLQQGIDMLLNSESGCVE 389
Query: 399 AVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAKVIERFVKKVPKLSHFTVSYV 458
A GYIS KL+EL+Q+F SF SR CLIFV+R I A+VI+R +KK+ L+HFTVS++
Sbjct: 390 AAKTGYISPKLYELIQIFHSFSNSRHARCLIFVDRKITARVIDRMIKKIGHLAHFTVSFL 449
Query: 459 TGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSY 518
TG SSV AL PK+QK+TL+SF GK+NLLF+TDV EEG+HVP CS V+RFDLP+T RSY
Sbjct: 450 TGGRSSVDALTPKMQKDTLDSFRSGKVNLLFTTDVAEEGIHVPECSCVIRFDLPRTTRSY 509
Query: 519 VQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMTDAAMSRDPDACILKPFSIVE 578
VQSRGRARQ +SQYIL++ERGN+KQ D + ++RSE SM A SR+ E
Sbjct: 510 VQSRGRARQEDSQYILMIERGNVKQNDLISAIVRSETSMVKIASSRESGNLSPGFVPNEE 569
Query: 579 TDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPIFQLSYGEGSYECRLTLPPTA 638
+ Y V TTGA VTADSS+ ++Y+YC+KLP DK +SPKP F+ ++ + Y C L LPP+A
Sbjct: 570 INEYHVGTTGAKVTADSSISIVYRYCEKLPQDKCYSPKPTFEFTHHDDGYVCTLALPPSA 629
Query: 639 AFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSR 698
Q +VGP +RN + +KQLVCL+ACKKLH+LGAL+DHL +E+ + N T
Sbjct: 630 VLQILVGPKARNMHKAKQLVCLDACKKLHELGALDDHLCLSVED--PVPEIVSKNKGTG- 689
Query: 699 AGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSCSVVYEIYSGFVLLIESKLDD 758
GTTKRKELHG T I A SG W Q+YK +F C +IYS FVLLI++ L D
Sbjct: 690 IGTTKRKELHGTTRIHAWSGNWVSKKTALKLQSYKMNFVCDQAGQIYSEFVLLIDATLPD 749
Query: 759 DVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGG 818
+V +E++LYL K VK SVS G + LDA+Q+ +AK FQ L FNG+FG+LF SK+
Sbjct: 750 EVATLEIDLYLHDKMVKTSVSSCGLLELDAQQMEQAKLFQGLLFNGLFGKLFTRSKVPNA 809
Query: 819 KREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVEFLKKNSSLD 878
REF+L K+ +W T+++YLLLP T+ S I+W +D+ + V+ +++ S +
Sbjct: 810 PREFILNKEDTFVWNTASVYLLLP---TNPSFDSNVCINWSVIDAAATAVKLMRRIYSEN 869
Query: 879 TESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNT 938
G S N LIH AN +SLKD+VVLA+HTGK+Y+ ++ ++
Sbjct: 870 KRELLGIFDSDQN-------VGDLIHLANKSCKANSLKDMVVLAVHTGKIYTALD-ITEL 929
Query: 939 SAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLLVN-FNDEG 998
S +S FDG SD+ + + FA+YFKKKYGI LR+P QP+L LK SHNPHNLL + F DEG
Sbjct: 930 SGDSAFDGASDKKECKFRTFAEYFKKKYGIVLRHPSQPLLVLKPSHNPHNLLSSKFRDEG 989
Query: 999 ETSDKLQSG-HASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLR 1058
+ + +G K VHMPPEL++ +D+P + +S YL P+LM+R+ESL LASQLR
Sbjct: 990 NVVENMSNGTPVVNKTSNRVHMPPELLIPLDLPVEILRSFYLFPALMYRIESLTLASQLR 1049
Query: 1059 KEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHE 1118
EI Y ++ ISS LI+EA+TTL C E FS+ERLELLGDSVLKYAVSCHLFLKFP K E
Sbjct: 1050 SEIGYSDSN--ISSFLILEAITTLRCSEDFSMERLELLGDSVLKYAVSCHLFLKFPNKDE 1109
Query: 1119 GQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCGVDTVE 1178
GQLSS R +ICNATL++LGI+ +QGY+RD+AFDPRRW+APGQ + RP PC+C V + E
Sbjct: 1110 GQLSSIRCHMICNATLYKLGIERNVQGYVRDAAFDPRRWLAPGQLSIRPSPCECPVKS-E 1169
Query: 1179 VPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLG 1238
V DD +D +VLGK CDKGHRW+ SKTIADCVEA+IGAYY G+ AA+ V+KWLG
Sbjct: 1170 VVTDDIHIIDDKAIVLGKACDKGHRWMCSKTIADCVEAIIGAYYAGGGLRAAMAVLKWLG 1229
Query: 1239 IDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGV 1298
I A++ L+V AI SAS+++ + K+ E LE+K+GY F+VKGLL+EA+TH S QELG
Sbjct: 1230 IGAEIEEDLIVQAILSASVQTYLPKDNVFEMLEAKLGYSFSVKGLLVEALTHPSQQELGA 1289
Query: 1299 NYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQH 1358
YCY+RLEFLGD+VLD+L+T + + +H D + GELTDLRSASVNNENFAQVAV+ N
Sbjct: 1290 KYCYERLEFLGDAVLDILLTRYLFNSHKDTNEGELTDLRSASVNNENFAQVAVKHNFHHF 1349
Query: 1359 LQHCCGLLIGQITEYVKYL-SESLDAGKSLHGN--KGPKVLGDMVESIAGAILIDTELNL 1418
LQH GLL+ QITEYV L S+D + L KGPKVLGD+VESIAGAIL+DT+L+L
Sbjct: 1350 LQHSSGLLLDQITEYVNRLEGSSMDKVELLSDGLPKGPKVLGDIVESIAGAILLDTKLDL 1409
Query: 1419 DEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQD 1478
D VW I++PLL+P VTP+ LEL P RELIE C GYF+ C +G+ + A L +QL++
Sbjct: 1410 DVVWGIFEPLLSPIVTPENLELPPYRELIEWCGKHGYFVGINCRDQGDTVVATLDVQLKE 1469
Query: 1479 VLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVGECHLPGSWLR 1538
VLL+ +G+ + RK A+ A+ LL LE +G+ ++ S K +Q H G + P + L
Sbjct: 1470 VLLVRQGFSKKRKDAKAHASSLLLKDLEEKGLIIPKNAS-KTEQFEKHCGSTN-PFNNLH 1529
Query: 1539 LIAFVAHF-----------------AVIGP-----VSLKKGGPRMTLFELCKKLQWPMPT 1598
+ A ++G V KGGPR+ L+ELCKKLQWPMPT
Sbjct: 1530 VDAMDTQTPKPTKEKNAADSRNISDPLLGKPLHVIVKTSKGGPRIALYELCKKLQWPMPT 1589
Query: 1599 FNTVE---NKSRVPMEFGEGSERRT-GFNSFWSNIILQIPNAGSIECEGDARADKKSSLD 1633
+ + + S V G S++ T +F S I L IPNA I GD RADKKSS D
Sbjct: 1590 MESEKVQPSFSSVCSSPGGSSQKATPQAFAFASTITLHIPNADVISLTGDGRADKKSSQD 1649
BLAST of MC08g0468 vs. ExPASy Swiss-Prot
Match:
Q9LXW7 (Endoribonuclease Dicer homolog 3 OS=Arabidopsis thaliana OX=3702 GN=DCL3 PE=1 SV=2)
HSP 1 Score: 1491.5 bits (3860), Expect = 0.0e+00
Identity = 834/1635 (51.01%), Postives = 1081/1635 (66.12%), Query Frame = 0
Query: 4 LEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAVLET 63
+EE + + KR FS+++ D D Y+ +V+EV RN IAVL T
Sbjct: 10 MEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSN---GSYELKVYEVAKNRNIIAVLGT 69
Query: 64 GAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYYGAM 123
G K+ I LIK +G S K+ IIFLAPTV+LV QQ I+A + +V EY+GA
Sbjct: 70 GIDKSEITKRLIKAMG---SSDTDKRLIIFLAPTVNLVKQQCCEIRALVNLKVEEYFGAK 129
Query: 124 GVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTK 183
GVD W+ + W++E S+HDVLVMTPQILLD LR AFL L VCL+IIDECH TGNHPY K
Sbjct: 130 GVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAK 189
Query: 184 IMKDFYHKSDNKPKIFGMTASPVIRKG-VSSSSDCECQIADLESIMDSQIYAIEDKKEVE 243
+MK+FYH+S +KPKIFG+TAS VIRKG VSS S+ Q+++LE +MDS+I+ E+++ VE
Sbjct: 190 LMKEFYHESTSKPKIFGLTASAVIRKGIVSSPSNYAAQVSELERLMDSKIFNPEEREGVE 249
Query: 244 IYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLK 303
+ + KE I Y P+ S S+ELK K+E KFDA L Q + + ++D+K + +
Sbjct: 250 KFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQ 309
Query: 304 KRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLV 363
KRLS D+ +I +CLD LGLICA+ A +V LE ++ T EE E ++E S+ K FLE+ L
Sbjct: 310 KRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILST 369
Query: 364 IGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVA 423
IG P ++++++ + AV G++S KL EL L +SF +Q CLI VERII A
Sbjct: 370 IGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITA 429
Query: 424 KVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEG 483
KVIERFVKK L++ V Y+T N S A K+Q E + F HGK+NLLF TDVVEEG
Sbjct: 430 KVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEG 489
Query: 484 LHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSM 543
VP+CS +V FDLPKT+ SY QS+ A+Q+NS+ I+ LERGN KQRD L D+MR E
Sbjct: 490 FQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRRE--- 549
Query: 544 TDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKP 603
+ +DP+A LK + + V+ G+ V DS++ + +
Sbjct: 550 ---VLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEE--------------- 609
Query: 604 IFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLL 663
A+ Q++ P SRN L CKKL L+++LL
Sbjct: 610 -------------------AASTQTMSDPPSRNEQ-------LPPCKKLR----LDNNLL 669
Query: 664 PLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFS 723
E + KS S+S AG+ KRKELHG T ALSGTWGE ++GA+FQAYKFDF
Sbjct: 670 Q--SNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFC 729
Query: 724 CSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCF 783
C++ E+YS F LL+ES L +DVG +E++LYL K VKASVS GQ+ L E++ KAK F
Sbjct: 730 CNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYF 789
Query: 784 QELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIH 843
Q+ FFNGMFG+LF+GSK G KREFLLQ DT LW + M+LLLPV+ D +S I
Sbjct: 790 QQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSA--TID 849
Query: 844 WRAVDSCVSVVEFLKKNSSLDTE----SNCGALPSRNNSAETGSKAASLIHFANCVLDVH 903
W A++SC S+VEFLKKNS LD + C + + + +LIHFAN D +
Sbjct: 850 WSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKN 909
Query: 904 SLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYP 963
SL++LVV+AIHTG++YSIVE VS++SA S F+ + A S Y +A+YF KKYGI L +P
Sbjct: 910 SLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVD---ASSGYATYAEYFNKKYGIVLAHP 969
Query: 964 QQPMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASK-KPQMHVHMPPELVVSMDVPKGV 1023
QP+++LKQSH+ HNLLV+FN+E + ++G+ K KP +H H+PPEL+ +DVP+ V
Sbjct: 970 NQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAV 1029
Query: 1024 WKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLE 1083
KS YLLPS+MHRLESLMLASQLR+EI+ ++ ISS+ I+EA+TTL C ESFS+ERLE
Sbjct: 1030 LKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLE 1089
Query: 1084 LLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDP 1143
LLGDSVLKY SCHLFLK+P+K EGQLS QRQ +I N+ LHRL KLQGYIR+ AF+P
Sbjct: 1090 LLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEP 1149
Query: 1144 RRWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCV 1203
RRW APGQ + PVPCKCG+DT EVPLD KF TE+ + +GK CD GHRWVVSK+++DC
Sbjct: 1150 RRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCA 1209
Query: 1204 EALIGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKI 1263
EALIGAYYV+ G+ A+LH++KWLGID D +LVV+AI SLR I KE E+ LE KI
Sbjct: 1210 EALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKI 1269
Query: 1264 GYEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELT 1323
+EF+ K LL EAITH+S +E +Y Y+RLEFLGDSVLD LIT H + T+ PGE+T
Sbjct: 1270 QHEFSAKFLLKEAITHSSLRE---SYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMT 1329
Query: 1324 DLRSASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPK 1383
DLRSA VNNENFAQVAV+ NL HLQ C +L QI +Y+ + + G+S+ +GPK
Sbjct: 1330 DLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPK 1389
Query: 1384 VLGDMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFI 1443
LGD+VESIAGA+LIDT L+LD+VWR+++PLL+P VTPDKL+L P REL ELCDSLGYF
Sbjct: 1390 ALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFF 1449
Query: 1444 KDKCTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGS 1503
+ KC+ G A ++LQL DVLL G+G E+T K A G+AA LL QLE R I SR S
Sbjct: 1450 RVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNI--SRKTS 1509
Query: 1504 KKRKQNP-------NHVGECHLPGSWLRLIAFVAHFAVIGPVSLKKGGPRMTLFELCKKL 1563
Q+ NH L + + V F IGP+++KKGGPR TL E CKK
Sbjct: 1510 LGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPF--IGPINMKKGGPRGTLHEFCKKH 1569
Query: 1564 QWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSS 1623
WPMPTF+T E KSR P EF +G E+RT F+SF S I L+IPN ++ G+AR DKKSS
Sbjct: 1570 LWPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSS 1573
Query: 1624 LDSASLVMLKELQRQ 1626
DSA + +L EL+R+
Sbjct: 1630 FDSAVVELLYELERR 1573
BLAST of MC08g0468 vs. ExPASy Swiss-Prot
Match:
Q7XD96 (Endoribonuclease Dicer homolog 3b OS=Oryza sativa subsp. japonica OX=39947 GN=DCL3B PE=3 SV=2)
HSP 1 Score: 1471.8 bits (3809), Expect = 0.0e+00
Identity = 788/1614 (48.82%), Postives = 1083/1614 (67.10%), Query Frame = 0
Query: 41 PRRYQ-TEVFEVGMRRNTIAVLETGAGKTMIAVMLIKEIGLTMKS-SGSKKYIIFLAPTV 100
PR Q EVFE +R NTIAVL+TG+GKTM+AVML +E +++ ++ ++FLAPTV
Sbjct: 38 PRTSQLVEVFEAALRGNTIAVLDTGSGKTMVAVMLAREHARRVRAGEAPRRIVVFLAPTV 97
Query: 101 HLVHQQFNVIKAHTDFEVAEYYGAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAF 160
HLVHQQF VI+ +TD +V GA V +W W++E ++++VMTPQILLDALR AF
Sbjct: 98 HLVHQQFEVIREYTDLDVMMCSGASRVGEWGADHWKEEVGRNEIVVMTPQILLDALRHAF 157
Query: 161 LSLGTVCLMIIDECHRATGNHPYTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCE 220
L++ V L+I DECHRA G+HPY +IMK+FY S +P +FGMTASPV KG S+ +CE
Sbjct: 158 LTMSAVSLLIFDECHRACGSHPYARIMKEFYFGSQWRPDVFGMTASPVATKGASTLHNCE 217
Query: 221 CQIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDA 280
I+ LE +D++IY +ED+ E+E + P + +Y+ LKSK++ +FD+
Sbjct: 218 AHISQLELTLDAKIYIVEDRNELESFSPPTTIVNKYYDAYMVDFDNLKSKLQIFSDEFDS 277
Query: 281 LLSNSQGAVQVHYKDVDSKLKPLKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVN-I 340
LL Q + +KD D+ L+ +K LS H KI Y L++LG I E +K+ +E+V +
Sbjct: 278 LLVGLQESPSNKFKDTDNILETSRKSLSRYHGKILYSLNDLGPIITSEVVKIHIESVKPL 337
Query: 341 TNEEYEVFRESSL-QYKCFLEEALLVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFE 400
+ E +F ++SL + + +EAL +I E P G ++ + GYISSK+
Sbjct: 338 CDSEDCIFSKASLCLHMSYFKEALSLIEEILPQGYGELMKSESGSEELTKRGYISSKVNT 397
Query: 401 LLQLFESFGVSRQVLCLIFVERIIVAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPK 460
L+ +F+SFG S +VLCLIFV+RII AK +ERF++ + S F++SY+TG ++S AL+P
Sbjct: 398 LINIFKSFGSSNEVLCLIFVDRIITAKAVERFMRGIVNFSCFSISYLTGGSTSKDALSPA 457
Query: 461 VQKETLESFCHGKLNLLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQ 520
VQ+ TL+ F GK+NLLF+TDV EEG+ VPNCS V+RFDLP+T+ SYVQSRGRAR+NNS+
Sbjct: 458 VQRFTLDLFRAGKVNLLFTTDVTEEGVDVPNCSCVIRFDLPRTVCSYVQSRGRARRNNSE 517
Query: 521 YILLLERGNLKQRDQLFDVMRSERSMTDAAMSRDPDACILKPFSIVETDCYIVETTGASV 580
+IL++ERGNL+Q++ +F ++++ + + A+ R P+A + SI Y V++TGA++
Sbjct: 518 FILMIERGNLQQQEHIFRMIQTGYYVKNCALYRHPNA-LSYDLSIQGMYTYQVQSTGATI 577
Query: 581 TADSSVGLIYQYCKKLPGDKYFSPKPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNS 640
TAD V LI +YC+KLP D+YF PKP F+++ +G ++C LTLP AAFQSIVGP S +S
Sbjct: 578 TADCCVNLIRKYCEKLPKDRYFMPKPSFEVTIEDGLFKCTLTLPRNAAFQSIVGPLSSSS 637
Query: 641 NLSKQLVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRT 700
NLSKQLV LEACKKLHQLG LNDHL+PL EE + D S GTTKRKELHG T
Sbjct: 638 NLSKQLVSLEACKKLHQLGELNDHLVPLTEEPMDTDFTTADEKCISGPGTTKRKELHGTT 697
Query: 701 AIGALSGTWGELVEGASFQAYKFDFSCSVVYEIYSGFVLLIESKLDDDVGNIELELYL-R 760
+ ALSGTW E + Y+ DF C E Y+GFVLL+E +LDDDV +++L+L
Sbjct: 698 CVLALSGTWIHDSENITLNTYRIDFLCDQEGENYAGFVLLMEPELDDDVAPSKMDLFLIP 757
Query: 761 SKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKP 820
+K V +V+ G+V L+ +Q+ K K FQE FFNG+FGRLF GS+ +G +R+F+ +K +
Sbjct: 758 NKMVYTTVTPRGKVQLNKKQLGKGKLFQEFFFNGIFGRLFHGSRKSGAQRDFIFKKGHEI 817
Query: 821 LWVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVEFLKKNSSLDTE---SNCGALP 880
W T +MYLLLP+ + DL IHW A++SC VE L + D NC +P
Sbjct: 818 QWNTESMYLLLPLRDSSYIQDDL-SIHWEAIESCAGAVEQLWSSYQGDENVIPVNC--IP 877
Query: 881 SRNNSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGN 940
+ + +IH AN L S+KD VVL++HTG++Y++++++ +T+AE +FD
Sbjct: 878 QKRRGGQ-----EEIIHLANKSLHCSSIKDSVVLSLHTGRIYTVLDLILDTTAEDSFDEM 937
Query: 941 SDRAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLLVNFND--EGETSDKLQS 1000
S + +F DY+ +KYGI +++P+QP+L LKQSHN HNLL + +G T L
Sbjct: 938 CKGKASPFTSFVDYYHQKYGIIIQHPEQPLLLLKQSHNAHNLLFSKLKYLDGSTGKPL-- 997
Query: 1001 GHASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEINYHSNS 1060
+K Q+H +PPEL++ +DV + KS YLLPS++HRL+SLMLASQLR+EI Y+ +
Sbjct: 998 --LMEKEQIHARVPPELLIHLDVTTDILKSFYLLPSVIHRLQSLMLASQLRREIGYNQH- 1057
Query: 1061 LCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQ 1120
I +LI+EA+TTL CCE+FSLERLELLGDSVLKY V C LFL++P KHEGQLS R +
Sbjct: 1058 --IPVTLILEAITTLRCCETFSLERLELLGDSVLKYVVGCDLFLRYPMKHEGQLSDMRSK 1117
Query: 1121 VICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCT 1180
+CNATLH+ GI LQGY+RD+AFDPRRWVAPGQ + RP PC CG++T VP ++
Sbjct: 1118 AVCNATLHKHGIWRSLQGYVRDNAFDPRRWVAPGQISLRPFPCNCGIETAFVPSHRRYIR 1177
Query: 1181 EDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDADLGVSL 1240
+DP +GK CD+GHRW+ SKTI+DCVEAL+GAYYV GI AAL V++W GID + L
Sbjct: 1178 DDPSFFVGKPCDRGHRWMCSKTISDCVEALVGAYYVGGGIAAALWVMRWFGIDIKCDMKL 1237
Query: 1241 VVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYCYQRLEF 1300
+ + +AS + K +IE LE+K+ Y F+VKGLLLEAITH S QELGV+YCYQRLEF
Sbjct: 1238 LQEVKFNASHLCSLSKINDIEELEAKLKYNFSVKGLLLEAITHPSLQELGVDYCYQRLEF 1297
Query: 1301 LGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQHCCGLLI 1360
LGDSVLDLL+T H Y TH+D+DPGELTDLRSA V+NENFAQ VR N+ HLQH G+L+
Sbjct: 1298 LGDSVLDLLLTRHLYATHTDVDPGELTDLRSALVSNENFAQAVVRNNIHSHLQHGSGILL 1357
Query: 1361 GQITEYVKYLSESLDAGKSLHGN---KGPKVLGDMVESIAGAILIDTELNLDEVWRIYKP 1420
QITEYV+ E + K PKVLGD++ESIAGA+ IDT+ N+D VW I++P
Sbjct: 1358 EQITEYVRSNLECQGKESEFLQHTTCKVPKVLGDIMESIAGAVFIDTDFNVDMVWEIFEP 1417
Query: 1421 LLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLIGEGYE 1480
LL+P +TPDKL L P REL+ELC +G F+ KCT KGE + E+ LQL+D LL+ +G++
Sbjct: 1418 LLSPLITPDKLALPPYRELLELCSHIGCFLNSKCTSKGEEVIIEMSLQLRDELLVAQGHD 1477
Query: 1481 RTRKAARGEAAHRLLVQLETRGIYYSRSGSKKR------------------KQNPNHVGE 1540
R +K A+ +AA R+L L+ +G+ + SK + + + + + +
Sbjct: 1478 RNKKRAKAKAASRILADLKQQGLSIKQCLSKAKQLDIVTSDLQFDLTSSGTQLSYSDLND 1537
Query: 1541 CHLPGSWLRLIAFVAHFAVIGPVSLKKGGPRMTLFELCKKLQWPMPTFNTVENKSRVPME 1600
H+ L ++ V V+ P+ ++KGGPR LF+LCK LQWPMP F VE + R P+
Sbjct: 1538 YHI----LEGLSSVKKEVVL-PLKMEKGGPRSALFKLCKILQWPMPEFEFVEQRFRTPIV 1597
Query: 1601 FGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLDSASLVMLKELQ 1624
+ T FNSF S I L IP+A +I +G+ R DKKS+ DSASL+ML +LQ
Sbjct: 1598 MDGAT--TTNFNSFVSTITLHIPDATTITFQGERRTDKKSAQDSASLMMLHKLQ 1628
BLAST of MC08g0468 vs. ExPASy Swiss-Prot
Match:
Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)
HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-225
Identity = 578/1727 (33.47%), Postives = 862/1727 (49.91%), Query Frame = 0
Query: 42 RRYQTEVFEVGMRRNTIAVLETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLV 101
RRYQ +V E +NTIA LETGAGKT+IA++LIK + + S K +FL P V LV
Sbjct: 250 RRYQLDVLEQAKAKNTIAFLETGAGKTLIAILLIKSVHKDLMSQNRKMLSVFLVPKVPLV 309
Query: 102 HQQFNVIKAHTDFEVAEYYGAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSL 161
+QQ VI+ T F+V Y G MG D W + W++E VLVMT QILL+ LR + + +
Sbjct: 310 YQQAEVIRNQTCFQVGHYCGEMGQDFWDSRRWQREFESKQVLVMTAQILLNILRHSIIRM 369
Query: 162 GTVCLMIIDECHRATGNHPYTKIMKDFYHKS--DNKPKIFGMTASPVIRKGVSSSSDCEC 221
T+ L+I+DECH A HPY+ +M +FYH + D +P IFGMTASPV KGVSS DC
Sbjct: 370 ETIDLLILDECHHAVKKHPYSLVMSEFYHTTPKDKRPAIFGMTASPVNLKGVSSQVDCAI 429
Query: 222 QIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAK---SPSMELKSKIEALWFKF 281
+I +LE+ +DS + I+D+KE+E +VP EI + Y+ A S +K I A+
Sbjct: 430 KIRNLETKLDSTVCTIKDRKELEKHVPMPSEIVVEYDKAATMWSLHETIKQMIAAVEEAA 489
Query: 282 DALLSNSQ----GAVQVHYKDVDSKLKPLKKRLSSDHL--------KISYCLDELGLICA 341
A S+ GA KD ++ + +R SD I+Y L ELG CA
Sbjct: 490 QASSRKSKWQFMGARDAGAKDELRQVYGVSERTESDGAANLIHKLRAINYTLAELGQWCA 549
Query: 342 Y---EAIKVLLENVNITNEEYEV-FRESSLQYKCFLEEALLVIG---------------- 401
Y ++ L++ N + +V F+ES L L + L+ G
Sbjct: 550 YKVGQSFLSALQSDERVNFQVDVKFQESYLSEVVSLLQCELLEGAAAEKVAAEVGKPENG 609
Query: 402 ------ESFPLGNENVLNFG--LD--IWKAVDLGYISSKLFELLQLFESFGVSRQVLCLI 461
E L ++ V++ G +D I AV G ++ K+ L++L + + ++
Sbjct: 610 NAHDEMEEGELPDDPVVSGGEHVDEVIGAAVADGKVTPKVQSLIKLLLKYQHTADFRAIV 669
Query: 462 FVERIIVAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLF 521
FVER++ A V+ + ++P LS + + G N+S + ++Q +T+ F G + LL
Sbjct: 670 FVERVVAALVLPKVFAELPSLSFIRCASMIGHNNSQEMKSSQMQ-DTISKFRDGHVTLLV 729
Query: 522 STDVVEEGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFD 581
+T V EEGL + C+ V+RFDL KT+ +Y+QSRGRAR+ S YIL++ERGN+ L +
Sbjct: 730 ATSVAEEGLDIRQCNVVMRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNVSHAAFLRN 789
Query: 582 VMRSERSMTDAAMSRD-----PDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYC 641
SE ++ A+ R D L V Y VE TGA V+ +S+VGL++ YC
Sbjct: 790 ARNSEETLRKEAIERTDLSHLKDTSRLISIDAVPGTVYKVEATGAMVSLNSAVGLVHFYC 849
Query: 642 KKLPGDKYFSPKPIFQLSY-----GEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVC 701
+LPGD+Y +P F + G Y CRL LP A F+ + GP + L++Q VC
Sbjct: 850 SQLPGDRYAILRPEFSMEKHEKPGGHTEYSCRLQLPCNAPFEILEGPVCSSMRLAQQAVC 909
Query: 702 LEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGT 761
L ACKKLH++GA D LLP + + A + + GT + +E + L G
Sbjct: 910 LAACKKLHEMGAFTDMLLPDKGSGQDAEKADQDDEGEPVPGTARHREFYPEGVADVLKGE 969
Query: 762 W----GELVEGAS-FQAYKFDFSC-------SVVYEIYSGFVLLIESKLDDDVGNIELEL 821
W E+ E + F Y ++ C S F +L ++LD +V ++ ++L
Sbjct: 970 WVSSGKEVCESSKLFHLYMYNVRCVDFGSSKDPFLSEVSEFAILFGNELDAEVLSMSMDL 1029
Query: 822 YL-RSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGKREFLLQK 881
Y+ R+ KAS++F G + + Q+ K F + +L
Sbjct: 1030 YVARAMITKASLAFKGSLDITENQLSSLKKFHVRLMS------------------IVLDV 1089
Query: 882 DTKPL---WVTSNMYLLLPVDLTDASSHDLWK-IHWRAVDSCVSVVEF------------ 941
D +P W + YL +PV TD +S + K I+W V+ +
Sbjct: 1090 DVEPSTTPWDPAKAYLFVPV--TDNTSMEPIKGINWELVEKITKTTAWDNPLQRARPDVY 1149
Query: 942 -------------------LKKN-----------------SSLDTESNCGALPSRN---N 1001
L+ N +S D G LP R+
Sbjct: 1150 LGTNERTLGGDRREYGFGKLRHNIVFGQKSHPTYGIRGAVASFDVVRASGLLPVRDAFEK 1209
Query: 1002 SAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSD-R 1061
E L+ C++ L +V A H+GK + + + + SAE++F
Sbjct: 1210 EVEEDLSKGKLMMADGCMV-AEDLIGKIVTAAHSGKRFYVDSICYDMSAETSFPRKEGYL 1269
Query: 1062 AQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLL-VNFNDEGETSDKLQSGHAS 1121
+Y +ADY+K+KYG+ L QQP+++ + NLL F GE+ L +
Sbjct: 1270 GPLEYNTYADYYKQKYGVDLNCKQQPLIKGRGVSYCKNLLSPRFEQSGESETVLDKTY-- 1329
Query: 1122 KKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCIS 1181
+V +PPEL V + + + LPS+M R+ES++LA QL+ I+Y I
Sbjct: 1330 -----YVFLPPELCVVHPLSGSLIRGAQRLPSIMRRVESMLLAVQLKNLISYP-----IP 1389
Query: 1182 SSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICN 1241
+S I+EALT C E+F ER ELLGD+ LK+ VS LFLK+P+KHEGQL+ RQQ++ N
Sbjct: 1390 TSKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVSN 1449
Query: 1242 ATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCG----VDTVEVPLDDKFCT 1301
L++ + LQ YI+ F P RW APG K G D + P+ ++
Sbjct: 1450 MVLYQFALVKGLQSYIQADRFAPSRWSAPGVPPVFDEDTKDGGSSFFDEEQKPVSEENSD 1509
Query: 1302 --EDPKVVLGKCCD--KGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDADL 1361
ED ++ G+ +R + SKT+AD VEALIG YYV G AA H++KW+GI +
Sbjct: 1510 VFEDGEMEDGELEGDLSSYRVLSSKTLADVVEALIGVYYVEGGKIAANHLMKWIGIHVED 1569
Query: 1362 GVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYCYQ 1421
V + + ++ +LK + LE + YEF KGLL+EAITH S GV+ CYQ
Sbjct: 1570 DPDEVDGTLKNVNVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVS-CYQ 1629
Query: 1422 RLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQHCC 1481
RLEF+GD+VLD LIT H + T++ + PG LTDLR+A+VNNENFA+VAV+ L +L+H
Sbjct: 1630 RLEFVGDAVLDHLITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGS 1689
Query: 1482 GLLIGQITEYVKYL-SESLDAGKSLHG---NKGPKVLGDMVESIAGAILIDTELNLDEVW 1541
L QI E+VK + +ES G + G K PKVLGD+VESIAGAI +D+ + W
Sbjct: 1690 SALEKQIREFVKEVQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAW 1749
Query: 1542 RIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLI 1601
++++PLL P VTP+ L + P+REL E C ++ K +R G E+ + D + +
Sbjct: 1750 KVFQPLLQPMVTPETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFI---DGVQV 1809
Query: 1602 GEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVGECHLPGSWLRLIAF 1625
G +K A+ AA L L+ + I + SK++ N + GE
Sbjct: 1810 GVAQNPQKKMAQKLAARNALAALKEKEI----AESKEKHINNGNAGEDQGENE------- 1869
BLAST of MC08g0468 vs. ExPASy Swiss-Prot
Match:
Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)
HSP 1 Score: 733.0 bits (1891), Expect = 7.3e-210
Identity = 555/1739 (31.91%), Postives = 846/1739 (48.65%), Query Frame = 0
Query: 42 RRYQTEVFEVGMRRNTIAVLETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLV 101
R+YQ EV E RNTIA LETGAGKT+IAV+LIK + M K +FL P V LV
Sbjct: 268 RQYQLEVLEQAKSRNTIAFLETGAGKTLIAVLLIKSVCDKMLKENKKMLAVFLVPKVPLV 327
Query: 102 HQQFNVIKAHTDFEVAEYYGAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSL 161
+Q VLVMT QILL+ LR + + +
Sbjct: 328 YQ--------------------------------------VLVMTAQILLNILRHSIIKM 387
Query: 162 GTVCLMIIDECHRATGNHPYTKIMKDFYHKS--DNKPKIFGMTASPVIRKGVSSSSDCEC 221
+ L+I+DECH A HPY+ +M +FYH + + +P +FGMTASPV KGV+S DC
Sbjct: 388 DAIHLLILDECHHAVKKHPYSLVMSEFYHTTPKEKRPAVFGMTASPVNLKGVTSQEDCAI 447
Query: 222 QIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKS------PSMELKSKIEALW 281
+I +LES +DS + I+D+KE+E +VP E+ + Y+ A + +++S +E
Sbjct: 448 KIRNLESKLDSVVCTIKDRKELEKHVPMPLEVVVQYDKAATLWSLHEQIKQMESTVE--- 507
Query: 282 FKFDALLSNSQ-------GAVQVHYKDVDSKLKPLKKRLSSDHL--------KISYCLDE 341
+A LS+S+ GA +D + + +R SD I+Y L E
Sbjct: 508 ---EAALSSSKRTKWQFMGARDAGSRDELRLVYGVSERTESDGAANLIQKLRAINYALGE 567
Query: 342 LGLICAY---EAIKVLLENVNITNEEYEV-FRESSLQ-----YKCFLEEALLVIGESFPL 401
LG CAY ++ L+N N + +V F+ES L+ C L E + E+ +
Sbjct: 568 LGQWCAYKVAQSFLTALQNDERANYQVDVKFQESYLKKVVDLLHCQLTEGAAMKSETSDV 627
Query: 402 GNENVLNFGLD------------------IWKAVDLGYISSKLFELLQLFESFGVSRQVL 461
+N + I AV G ++ ++ L+++ + +
Sbjct: 628 EMQNTEKHNTNDLEEGELPDSHGEHVDEVIGAAVADGKVTPRVQALIKILLKYQHTEDFR 687
Query: 462 CLIFVERIIVAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLN 521
+IFVER++ A V+ + + ++P LS + + G N++ A ++Q +T+ F G++
Sbjct: 688 AIIFVERVVTALVLPKVLAELPSLSFIRCASLIGHNNNQEMRACQMQ-DTISKFRDGRVT 747
Query: 522 LLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQ 581
LL +T V EEGL + C+ V+RFDL KT+ +Y+QSRGRAR+ S YIL+LERGN+
Sbjct: 748 LLVATSVAEEGLDIRQCNVVIRFDLAKTVLAYIQSRGRARKPGSDYILMLERGNISHETF 807
Query: 582 LFDVMRSERSMTDAAMSRD-----PDACILKPFSIVETDCYIVETTGASVTADSSVGLIY 641
L + SE ++ AM R +L P Y VE+TGA V+ +S+VGLI+
Sbjct: 808 LRNARNSEETLRKEAMERTDLSHLDGTSVLSPVDTSPGSMYQVESTGAVVSLNSAVGLIH 867
Query: 642 QYCKKLPGDKYFSPKPIFQLSY-----GEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQ 701
YC +LP D+Y P F + G Y C+L LP A F+ + GP + L++Q
Sbjct: 868 FYCSQLPSDRYSILHPEFIMQKYEKPGGSVEYSCKLQLPCNAPFEKLEGPICSSIRLAQQ 927
Query: 702 LVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGAL 761
VCL ACKKLH++GA D LLP E + +++ GT + +E + L
Sbjct: 928 AVCLAACKKLHEMGAFTDTLLP-DRGSGEGEKTEQNDEGEPLPGTARHREFYPEGVADIL 987
Query: 762 SGTWGELVEGAS-------FQAYKFDFSCSVV-------YEIYSGFVLLIESKLDDDVGN 821
G W ++ G + Y + +C V S F ++ ++LD +V +
Sbjct: 988 RGEW--ILSGRDGYQNSQFIKLYMYSVNCVNVGTSKDPFVTQLSNFAIIFGNELDAEVLS 1047
Query: 822 IELELYL-RSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGKRE 881
++L++ R+ KAS+ F G++ + Q+ K F +
Sbjct: 1048 TTMDLFVARTMITKASLVFRGRIEITESQLVLLKSFHVRLMS------------------ 1107
Query: 882 FLLQKDTKPL---WVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVE--------- 941
+L D P W + YL +PV + + L +I W V++ V+
Sbjct: 1108 IVLDVDVDPSTTPWDPAKAYLFVPVG-AEKCTDPLREIDWTLVNNIVNTDAWNNPLQRAR 1167
Query: 942 ---FLKKN------------------------------------SSLDTESNCGALPSRN 1001
+L N + D G +P+R+
Sbjct: 1168 PDVYLGTNERTLGGDRREYGFGKLRHGTAFGQKAHPTYGIRGAIAEFDIVKASGLVPARD 1227
Query: 1002 NSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSD- 1061
+ + + A+ + L +VV A H+GK + + + N +AE++F
Sbjct: 1228 RGHFSDYQNQGKLFMADSCWNAKDLAGMVVTAAHSGKRFYVDCICYNMNAENSFPRKEGY 1287
Query: 1062 RAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLL---VNFND--EGETSDKLQ 1121
+Y ++ADY+K+KYG+ L Y +QP++R + NLL +D EG+ S+ L
Sbjct: 1288 LGPLEYSSYADYYKQKYGVELIYRKQPLIRARGVSYCKNLLSPRFEHSDAREGDFSENLD 1347
Query: 1122 SGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEINYHSN 1181
+ +V++PPEL + +P + + LPS+M R+ES++LA QL+ I+Y
Sbjct: 1348 KTY-------YVYLPPELCLVHPLPGSLVRGAQRLPSIMRRVESMLLAVQLKDIIDYP-- 1407
Query: 1182 SLCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQ 1241
+ ++ I+EALT C E+ ER ELLGD+ LK+ VS LFLK+P+KHEGQL+ RQ
Sbjct: 1408 ---VPATKILEALTAASCQETLCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQ 1467
Query: 1242 QVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPG--------QRTTRP-------VPCK 1301
Q++ N L++ + LQ YI+ F P RW APG R P C+
Sbjct: 1468 QMVSNMVLYQYALNKTLQSYIQADRFAPSRWAAPGVLPVFDEESREYEPSIFDEESTGCE 1527
Query: 1302 CGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAAL 1361
++ + D+ ED ++ C +R + SKT+AD VEALIG YYV G AA
Sbjct: 1528 LQKESYDDYADN--MQEDGEIEGDSSC---YRVLSSKTLADVVEALIGVYYVAGGKIAAN 1587
Query: 1362 HVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHT 1421
H++KW+GI A+L + + I++ +TL+ +G EF KGLL+EAITH
Sbjct: 1588 HLMKWIGIHAELDPE-EIPPPKPYDIPESIMRSINFDTLKGVLGIEFQNKGLLVEAITHA 1647
Query: 1422 SDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAV 1481
S GV+ CYQRLEF+GD+VLD LIT H + T++D+ PG LTDLR+A+VNNENFA+VAV
Sbjct: 1648 SRPSSGVS-CYQRLEFVGDAVLDHLITRHLFFTYTDLPPGRLTDLRAAAVNNENFARVAV 1707
Query: 1482 RRNLQQHLQHCCGLLIGQITEYVKYLSES-LDAGKSLHG---NKGPKVLGDMVESIAGAI 1541
+ L HL+H L QI E+VK + E L G + G K PKVLGD+VESIAGAI
Sbjct: 1708 KHKLHVHLRHGSSALETQIREFVKDVQEELLKPGFNSFGLGDCKAPKVLGDIVESIAGAI 1767
Query: 1542 LIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHA 1601
+D+ + VW++++PLL P VTP+ L + P+REL E C ++ K +R G I
Sbjct: 1768 FLDSGYDTSVVWKVFQPLLHPMVTPETLPMHPVRELQERCQQQAEGLEYKASRAGNIATV 1827
Query: 1602 ELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVGEC 1625
E+ D + IG +K A+ AA LV L+ + + +KK + GE
Sbjct: 1828 EV---FVDGVQIGVAQNPQKKMAQKLAARNALVVLKEK-----ETATKKEDERD---GE- 1872
BLAST of MC08g0468 vs. NCBI nr
Match:
XP_022132010.1 (endoribonuclease Dicer homolog 3a isoform X1 [Momordica charantia])
HSP 1 Score: 3192 bits (8276), Expect = 0.0
Identity = 1615/1661 (97.23%), Postives = 1620/1661 (97.53%), Query Frame = 0
Query: 1 MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV 60
MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV
Sbjct: 1 MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV 60
Query: 61 LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY 120
LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY
Sbjct: 61 LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY 120
Query: 121 GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP 180
GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP
Sbjct: 121 GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP 180
Query: 181 YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE 240
YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE
Sbjct: 181 YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE 240
Query: 241 VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP 300
VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP
Sbjct: 241 VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP 300
Query: 301 LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL 360
LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL
Sbjct: 301 LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL 360
Query: 361 LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII 420
LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII
Sbjct: 361 LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII 420
Query: 421 VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE 480
VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE
Sbjct: 421 VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE 480
Query: 481 EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER 540
EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER
Sbjct: 481 EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER 540
Query: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP 600
SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP
Sbjct: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP 600
Query: 601 KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH 660
KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH
Sbjct: 601 KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH 660
Query: 661 LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD 720
LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD
Sbjct: 661 LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD 720
Query: 721 FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK 780
FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK
Sbjct: 721 FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK 780
Query: 781 CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK 840
CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK
Sbjct: 781 CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK 840
Query: 841 IHWRAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSL 900
IHWRAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSL
Sbjct: 841 IHWRAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSL 900
Query: 901 KDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQ 960
KDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQ
Sbjct: 901 KDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQ 960
Query: 961 PMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKS 1020
PMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKS
Sbjct: 961 PMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKS 1020
Query: 1021 TYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLG 1080
TYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLG
Sbjct: 1021 TYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLG 1080
Query: 1081 DSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRW 1140
DSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRW
Sbjct: 1081 DSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRW 1140
Query: 1141 VAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEAL 1200
VAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEAL
Sbjct: 1141 VAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEAL 1200
Query: 1201 IGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYE 1260
IGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYE
Sbjct: 1201 IGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYE 1260
Query: 1261 FAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLR 1320
FAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLR
Sbjct: 1261 FAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLR 1320
Query: 1321 SASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLG 1380
SASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLG
Sbjct: 1321 SASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLG 1380
Query: 1381 DMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDK 1440
DMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDK
Sbjct: 1381 DMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDK 1440
Query: 1441 CTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKR 1500
CTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKR
Sbjct: 1441 CTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKR 1500
Query: 1501 KQNPNHVGECHLPGSWLRLI------------AFVAHFA----------------VIGPV 1560
KQNPNHV + S + + + H + VIGPV
Sbjct: 1501 KQNPNHVESALVLPSSINCSTEACDPNLETPDSVMVHSSATNCSTEGYSPGMDIPVIGPV 1560
Query: 1561 SLKKGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIP 1620
SLKKGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIP
Sbjct: 1561 SLKKGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIP 1620
Query: 1621 NAGSIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS 1633
NAGSIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS
Sbjct: 1621 NAGSIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS 1661
BLAST of MC08g0468 vs. NCBI nr
Match:
XP_022132011.1 (endoribonuclease Dicer homolog 3a isoform X2 [Momordica charantia])
HSP 1 Score: 2906 bits (7533), Expect = 0.0
Identity = 1471/1517 (96.97%), Postives = 1476/1517 (97.30%), Query Frame = 0
Query: 145 MTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTKIMKDFYHKSDNKPKIFGMTAS 204
MTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTKIMKDFYHKSDNKPKIFGMTAS
Sbjct: 1 MTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTKIMKDFYHKSDNKPKIFGMTAS 60
Query: 205 PVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKSPSME 264
PVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKSPSME
Sbjct: 61 PVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKSPSME 120
Query: 265 LKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKKRLSSDHLKISYCLDELGLICA 324
LKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKKRLSSDHLKISYCLDELGLICA
Sbjct: 121 LKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKKRLSSDHLKISYCLDELGLICA 180
Query: 325 YEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVIGESFPLGNENVLNFGLDIWKA 384
YEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVIGESFPLGNENVLNFGLDIWKA
Sbjct: 181 YEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVIGESFPLGNENVLNFGLDIWKA 240
Query: 385 VDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAKVIERFVKKVPKLSHFTVSYVT 444
VDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAKVIERFVKKVPKLSHFTVSYVT
Sbjct: 241 VDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAKVIERFVKKVPKLSHFTVSYVT 300
Query: 445 GCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSYV 504
GCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSYV
Sbjct: 301 GCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSYV 360
Query: 505 QSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMTDAAMSRDPDACILKPFSIVET 564
QSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMTDAAMSRDPDACILKPFSIVET
Sbjct: 361 QSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMTDAAMSRDPDACILKPFSIVET 420
Query: 565 DCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPIFQLSYGEGSYECRLTLPPTAA 624
DCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPIFQLSYGEGSYECRLTLPPTAA
Sbjct: 421 DCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPIFQLSYGEGSYECRLTLPPTAA 480
Query: 625 FQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRA 684
FQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRA
Sbjct: 481 FQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRA 540
Query: 685 GTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSCSVVYEIYSGFVLLIESKLDDD 744
GTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSCSVVYEIYSGFVLLIESKLDDD
Sbjct: 541 GTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSCSVVYEIYSGFVLLIESKLDDD 600
Query: 745 VGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGK 804
VGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGK
Sbjct: 601 VGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGK 660
Query: 805 REFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVEFLKKNSSLDT 864
REFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVEFLKKNSSLDT
Sbjct: 661 REFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVEFLKKNSSLDT 720
Query: 865 ESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTS 924
ESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTS
Sbjct: 721 ESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTS 780
Query: 925 AESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLLVNFNDEGET 984
AESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLLVNFNDEGET
Sbjct: 781 AESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLLVNFNDEGET 840
Query: 985 SDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEI 1044
SDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEI
Sbjct: 841 SDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEI 900
Query: 1045 NYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQL 1104
NYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQL
Sbjct: 901 NYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQL 960
Query: 1105 SSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCGVDTVEVPL 1164
SSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCGVDTVEVPL
Sbjct: 961 SSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCGVDTVEVPL 1020
Query: 1165 DDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDA 1224
DDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDA
Sbjct: 1021 DDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDA 1080
Query: 1225 DLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYC 1284
DLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYC
Sbjct: 1081 DLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYC 1140
Query: 1285 YQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQH 1344
YQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQH
Sbjct: 1141 YQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQH 1200
Query: 1345 CCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMVESIAGAILIDTELNLDEVWRI 1404
CCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMVESIAGAILIDTELNLDEVWRI
Sbjct: 1201 CCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMVESIAGAILIDTELNLDEVWRI 1260
Query: 1405 YKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLIGE 1464
YKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLIGE
Sbjct: 1261 YKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLIGE 1320
Query: 1465 GYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVGECHLPGSWLRLI---- 1524
GYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHV + S +
Sbjct: 1321 GYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVESALVLPSSINCSTEAC 1380
Query: 1525 --------AFVAHFA----------------VIGPVSLKKGGPRMTLFELCKKLQWPMPT 1584
+ + H + VIGPVSLKKGGPRMTLFELCKKLQWPMPT
Sbjct: 1381 DPNLETPDSVMVHSSATNCSTEGYSPGMDIPVIGPVSLKKGGPRMTLFELCKKLQWPMPT 1440
Query: 1585 FNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLDSASL 1633
FNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLDSASL
Sbjct: 1441 FNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLDSASL 1500
BLAST of MC08g0468 vs. NCBI nr
Match:
XP_038885573.1 (endoribonuclease Dicer homolog 3 isoform X1 [Benincasa hispida])
HSP 1 Score: 2825 bits (7323), Expect = 0.0
Identity = 1414/1643 (86.06%), Postives = 1507/1643 (91.72%), Query Frame = 0
Query: 1 MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV 60
MH+ EE+NR+GA KR FSD+EPS ASD S+F Q MD+GFIPRRYQ EVFEVGMRRNTIAV
Sbjct: 1 MHEPEEENRDGAHKRKFSDLEPSAASDQSNFNQTMDEGFIPRRYQREVFEVGMRRNTIAV 60
Query: 61 LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY 120
LETG+GKTMIAVMLIKEIG MKSSG KK+IIFLAPTVHLVHQQFNVIK HTDFEVAEYY
Sbjct: 61 LETGSGKTMIAVMLIKEIGKAMKSSGDKKFIIFLAPTVHLVHQQFNVIKVHTDFEVAEYY 120
Query: 121 GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP 180
GAMGVDDW++ CW+KETSE DVLVMTPQILLDALRKA+ LGTVCLMIIDECHR TGNHP
Sbjct: 121 GAMGVDDWNMNCWDKETSERDVLVMTPQILLDALRKAYFRLGTVCLMIIDECHRVTGNHP 180
Query: 181 YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE 240
YTKIMK+FYHKSDNKPKIFGMTASPVIRKGVSSS DCECQIADLESI+DSQIYAIEDKKE
Sbjct: 181 YTKIMKEFYHKSDNKPKIFGMTASPVIRKGVSSSFDCECQIADLESILDSQIYAIEDKKE 240
Query: 241 VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP 300
VEIYVPSAKEICIFYEPAK ++ELK+KIEALW +FDALLSNS+GA+Q HYKDVDSKLK
Sbjct: 241 VEIYVPSAKEICIFYEPAKPQTVELKAKIEALWSEFDALLSNSKGAMQGHYKDVDSKLKA 300
Query: 301 LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL 360
LKKRLS+DHLKI+YCLDELG++CAYEAIKVLLENVN NEE EV+RES LQYK FLEEAL
Sbjct: 301 LKKRLSNDHLKITYCLDELGVMCAYEAIKVLLENVNFPNEEPEVYRESFLQYKRFLEEAL 360
Query: 361 LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII 420
LVIGESFPLGNEN+ NFG D WKA+D GYIS KLFELLQLFESFGVSRQVLCLIFVERII
Sbjct: 361 LVIGESFPLGNENIPNFGFDFWKAIDSGYISPKLFELLQLFESFGVSRQVLCLIFVERII 420
Query: 421 VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE 480
A VIER VKKV LSHFTVSYVTGCN+SVGALAPKVQKETL+ FCHGKLNLLFSTDVVE
Sbjct: 421 AANVIERIVKKVANLSHFTVSYVTGCNASVGALAPKVQKETLKLFCHGKLNLLFSTDVVE 480
Query: 481 EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER 540
EGLHVPNCS VVRFDLPKT+RSYVQSRGRARQNNSQYILLLERGNLKQR QL D++RS+R
Sbjct: 481 EGLHVPNCSFVVRFDLPKTVRSYVQSRGRARQNNSQYILLLERGNLKQRAQLCDIIRSDR 540
Query: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP 600
SMTDAAMSRDPDACILKPFSIVETDCYIVE TGASVTADSSVGLIYQYCKKLPGDKYFSP
Sbjct: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVEATGASVTADSSVGLIYQYCKKLPGDKYFSP 600
Query: 601 KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH 660
KPIFQLSYGEGSYEC+LTLPPTA FQ+IVGPS+RNSNLSKQLVCLEACK+LHQ+G+LNDH
Sbjct: 601 KPIFQLSYGEGSYECQLTLPPTAPFQTIVGPSTRNSNLSKQLVCLEACKRLHQMGSLNDH 660
Query: 661 LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD 720
LLP IEEISEFD LKSN STSRAG+TKRKELHGRTAI ALSGTWGE VEGA+FQAYKFD
Sbjct: 661 LLPSIEEISEFDVGLKSNASTSRAGSTKRKELHGRTAIRALSGTWGEQVEGATFQAYKFD 720
Query: 721 FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK 780
FSCSV+YE+YSGFVLLIESKLDDDVGN ELELYL SKTVKA VSFGG+VHLDAEQI KAK
Sbjct: 721 FSCSVMYELYSGFVLLIESKLDDDVGNFELELYLLSKTVKAFVSFGGEVHLDAEQITKAK 780
Query: 781 CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK 840
CFQELFFNG+FGRLFIGSK TG KR+FLLQKDTKP W++SNMYLLLP++L D S+HDLWK
Sbjct: 781 CFQELFFNGLFGRLFIGSKSTGKKRDFLLQKDTKPFWISSNMYLLLPINLPDDSTHDLWK 840
Query: 841 IHWRAVDSCVSVVEFLKKNSSLDTESNCGA------LPSRNNSAETGSKAASLIHFANCV 900
IHWRA+DSCVSVVEFLKKNSSLD E NCGA PSRNNS ETGSKAASLIHFANCV
Sbjct: 841 IHWRAIDSCVSVVEFLKKNSSLDAERNCGAGATSDSSPSRNNSTETGSKAASLIHFANCV 900
Query: 901 LDVHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGIT 960
LDVHSLKD VVLAIHTG++YSIVEVVSNTSAES FDGNSD+A DYI FADYF K+YGI+
Sbjct: 901 LDVHSLKDRVVLAIHTGRIYSIVEVVSNTSAESPFDGNSDKAPPDYITFADYFNKRYGIS 960
Query: 961 LRYPQQPMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVP 1020
L +P+QP+LRLKQSHNPHNLLVNF DEG TSDKL SG A +KPQMHVHMPPELV+SMDVP
Sbjct: 961 LSFPKQPLLRLKQSHNPHNLLVNFKDEGNTSDKLLSGPAFRKPQMHVHMPPELVLSMDVP 1020
Query: 1021 KGVWKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLE 1080
KGV KS+YLLPSLMHR ESLMLASQLRKEINYHSNSLCISSSLI+EALTTLG CE+FSLE
Sbjct: 1021 KGVLKSSYLLPSLMHRFESLMLASQLRKEINYHSNSLCISSSLILEALTTLGSCENFSLE 1080
Query: 1081 RLELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSA 1140
RLELLGDSVLKYAVSCHLFLKFPEKHEG LSS+RQQVICNATLH LG KC LQGYIRDSA
Sbjct: 1081 RLELLGDSVLKYAVSCHLFLKFPEKHEGHLSSRRQQVICNATLHALGTKCMLQGYIRDSA 1140
Query: 1141 FDPRRWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIA 1200
FDPRRWVAPGQ T RPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIA
Sbjct: 1141 FDPRRWVAPGQGTNRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIA 1200
Query: 1201 DCVEALIGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLE 1260
DC EALIGAYYV+ GI AALHV+KWLGIDADL V+LV DAITSASLRSC L++TEI TLE
Sbjct: 1201 DCAEALIGAYYVDGGIIAALHVMKWLGIDADLDVALVADAITSASLRSCPLEDTEIATLE 1260
Query: 1261 SKIGYEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPG 1320
SKIGY+F+VKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQ HS+IDPG
Sbjct: 1261 SKIGYDFSVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQNHSEIDPG 1320
Query: 1321 ELTDLRSASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNK 1380
ELTDLRSASVNNENFAQVAVRRNLQQHLQHC GLL+ Q+TEYVKYLSES D GKSLHGNK
Sbjct: 1321 ELTDLRSASVNNENFAQVAVRRNLQQHLQHCSGLLMSQVTEYVKYLSESHDTGKSLHGNK 1380
Query: 1381 GPKVLGDMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLG 1440
GPKVLGDMVESIAGAILIDT+LNLDEVW+IYKPLLTPFVTPDKLEL PLRELIELCDSLG
Sbjct: 1381 GPKVLGDMVESIAGAILIDTKLNLDEVWKIYKPLLTPFVTPDKLELPPLRELIELCDSLG 1440
Query: 1441 YFIKDKCTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSR 1500
YFIKDKCTRKGEI HAELRLQLQD LLIGEGYERTRKAARGEAAH LLVQLETRGI YSR
Sbjct: 1441 YFIKDKCTRKGEIFHAELRLQLQDALLIGEGYERTRKAARGEAAHCLLVQLETRGISYSR 1500
Query: 1501 SGSKKRKQNPNHVGECHLPGSWLRLIAFVA----HFAVIGPVSLKKGGPRMTLFELCKKL 1560
SGSKKRKQNP+H+ + S + VIGP++LKKGGPR TLFELCKKL
Sbjct: 1501 SGSKKRKQNPSHIEAAMVLPSSVNCSTEACDPNLETPVIGPINLKKGGPRSTLFELCKKL 1560
Query: 1561 QWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSS 1620
QWPMPTFNTVENKSRV +E GEGSE+R GFNSF SN+ILQIPN G IECEG+ARADKKSS
Sbjct: 1561 QWPMPTFNTVENKSRVQIEIGEGSEKRKGFNSFLSNVILQIPNVGCIECEGEARADKKSS 1620
Query: 1621 LDSASLVMLKELQRQGRVNINDS 1633
LDSASL+ML+EL+++GRV INDS
Sbjct: 1621 LDSASLIMLQELRQRGRVIINDS 1643
BLAST of MC08g0468 vs. NCBI nr
Match:
XP_022132012.1 (endoribonuclease Dicer homolog 3a isoform X3 [Momordica charantia])
HSP 1 Score: 2822 bits (7316), Expect = 0.0
Identity = 1429/1478 (96.68%), Postives = 1436/1478 (97.16%), Query Frame = 0
Query: 184 IMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEI 243
++ DFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEI
Sbjct: 5 VLLDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEI 64
Query: 244 YVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKK 303
YVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKK
Sbjct: 65 YVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKK 124
Query: 304 RLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVI 363
RLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVI
Sbjct: 125 RLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVI 184
Query: 364 GESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAK 423
GESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAK
Sbjct: 185 GESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAK 244
Query: 424 VIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGL 483
VIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGL
Sbjct: 245 VIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGL 304
Query: 484 HVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMT 543
HVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMT
Sbjct: 305 HVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMT 364
Query: 544 DAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPI 603
DAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPI
Sbjct: 365 DAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPI 424
Query: 604 FQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLP 663
FQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLP
Sbjct: 425 FQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLP 484
Query: 664 LIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSC 723
LIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSC
Sbjct: 485 LIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSC 544
Query: 724 SVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQ 783
SVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQ
Sbjct: 545 SVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQ 604
Query: 784 ELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHW 843
ELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHW
Sbjct: 605 ELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHW 664
Query: 844 RAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDL 903
RAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDL
Sbjct: 665 RAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDL 724
Query: 904 VVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPML 963
VVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPML
Sbjct: 725 VVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPML 784
Query: 964 RLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYL 1023
RLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYL
Sbjct: 785 RLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYL 844
Query: 1024 LPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSV 1083
LPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSV
Sbjct: 845 LPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSV 904
Query: 1084 LKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAP 1143
LKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAP
Sbjct: 905 LKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAP 964
Query: 1144 GQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGA 1203
GQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGA
Sbjct: 965 GQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGA 1024
Query: 1204 YYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAV 1263
YYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAV
Sbjct: 1025 YYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAV 1084
Query: 1264 KGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSAS 1323
KGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSAS
Sbjct: 1085 KGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSAS 1144
Query: 1324 VNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMV 1383
VNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMV
Sbjct: 1145 VNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMV 1204
Query: 1384 ESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTR 1443
ESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTR
Sbjct: 1205 ESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTR 1264
Query: 1444 KGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQN 1503
KGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQN
Sbjct: 1265 KGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQN 1324
Query: 1504 PNHVGECHLPGSWLRLI------------AFVAHFA----------------VIGPVSLK 1563
PNHV + S + + + H + VIGPVSLK
Sbjct: 1325 PNHVESALVLPSSINCSTEACDPNLETPDSVMVHSSATNCSTEGYSPGMDIPVIGPVSLK 1384
Query: 1564 KGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAG 1623
KGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAG
Sbjct: 1385 KGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAG 1444
Query: 1624 SIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS 1633
SIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS
Sbjct: 1445 SIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS 1482
BLAST of MC08g0468 vs. NCBI nr
Match:
XP_023547257.1 (endoribonuclease Dicer homolog 3a isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2793 bits (7239), Expect = 0.0
Identity = 1414/1641 (86.17%), Postives = 1498/1641 (91.29%), Query Frame = 0
Query: 1 MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV 60
MH+ EE+N +GARKRNF+DME S ASD ++F +PMD+GFIPRRYQ EVFEVGM+RNTIAV
Sbjct: 1 MHNPEEENCDGARKRNFNDMESSVASDQTNFDKPMDEGFIPRRYQMEVFEVGMKRNTIAV 60
Query: 61 LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY 120
LETGAGKTMIAVMLIKEIG MKSSG KK+IIFLAPTVHLVHQQFNVIK TDFEVAEYY
Sbjct: 61 LETGAGKTMIAVMLIKEIGKAMKSSGDKKFIIFLAPTVHLVHQQFNVIKVQTDFEVAEYY 120
Query: 121 GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP 180
GAMGVDDW++ CWEKETSE DVLVMTPQILLDALRKA+ LGTVCLMI+DECHRATGNHP
Sbjct: 121 GAMGVDDWNMNCWEKETSERDVLVMTPQILLDALRKAYFRLGTVCLMIMDECHRATGNHP 180
Query: 181 YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE 240
YTKIMK+FYHKSDNKPKIFGMTASPVIRKGVSSS DCECQI +LESI+DS+IYAIEDKKE
Sbjct: 181 YTKIMKEFYHKSDNKPKIFGMTASPVIRKGVSSSLDCECQIGELESILDSKIYAIEDKKE 240
Query: 241 VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP 300
VEIYVPSAKE CIFYEPAKS ++ELK+KIEALW KFD LLSNSQ A+QVHYKDVDSKLK
Sbjct: 241 VEIYVPSAKETCIFYEPAKSLTLELKAKIEALWSKFDTLLSNSQVALQVHYKDVDSKLKA 300
Query: 301 LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL 360
LKKRLSSD KISYCLDELG++CAYEAIKVLLENVNI N EYE+ RESSLQYK FLEE L
Sbjct: 301 LKKRLSSDRFKISYCLDELGVLCAYEAIKVLLENVNIPNNEYELSRESSLQYKQFLEEEL 360
Query: 361 LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII 420
VIGESFPLGNEN+ NFG D KAVDLGYIS KLFELLQLFESFGVSRQVLCLIFVERII
Sbjct: 361 RVIGESFPLGNENIPNFGFDSQKAVDLGYISPKLFELLQLFESFGVSRQVLCLIFVERII 420
Query: 421 VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE 480
A VIERFVKKV LSHFTVSYVTGCN+SVGALAPKVQKETLE F HGK+NLLFSTDVVE
Sbjct: 421 AANVIERFVKKVANLSHFTVSYVTGCNTSVGALAPKVQKETLELFRHGKVNLLFSTDVVE 480
Query: 481 EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER 540
EGLHVPNCS VVRFDLPKT+RSYVQSRGRARQ+NSQYILLLERGN+KQR+QLFDV+RS+R
Sbjct: 481 EGLHVPNCSFVVRFDLPKTVRSYVQSRGRARQSNSQYILLLERGNVKQREQLFDVIRSDR 540
Query: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP 600
SMT+AAMSRDPDAC+LKPFSI+ETD YIVE TGASVTADSSVGLI+ YCKKLPGDKYFSP
Sbjct: 541 SMTEAAMSRDPDACMLKPFSIMETDSYIVEATGASVTADSSVGLIHLYCKKLPGDKYFSP 600
Query: 601 KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH 660
KPIFQLSYGEGSYECRL LPPTAAFQ+IVGPS+RNSNLSKQLVCLEACKKLHQ+GALNDH
Sbjct: 601 KPIFQLSYGEGSYECRLILPPTAAFQTIVGPSTRNSNLSKQLVCLEACKKLHQMGALNDH 660
Query: 661 LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD 720
LLP IEEISEFD LK N STS AGTTKRKELHGRTAI ALSGTWGE VEGA+FQAYKFD
Sbjct: 661 LLPSIEEISEFDMGLKCNASTSGAGTTKRKELHGRTAIRALSGTWGEKVEGATFQAYKFD 720
Query: 721 FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK 780
FSCSV+YE +SGFVLL+ESKLDDDVGN ELELYL SK VKASVSF GQVHLDAEQI KAK
Sbjct: 721 FSCSVIYEHFSGFVLLLESKLDDDVGNFELELYLISKMVKASVSFAGQVHLDAEQIMKAK 780
Query: 781 CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK 840
CFQELFFNG+FGRL IGSKLTG KR+FLLQKDTKPLW+TSNMYLLLPVD DASS DLWK
Sbjct: 781 CFQELFFNGLFGRLLIGSKLTGKKRDFLLQKDTKPLWITSNMYLLLPVDSPDASSDDLWK 840
Query: 841 IHWRAVDSCVSVVEFLKKNSSLDTESNCGAL----PSRNNSAETGSKAASLIHFANCVLD 900
IHW A+DSCVSVVEFLKKNSSLD E NCGA+ PSRN SAET S AASLIHFANCVLD
Sbjct: 841 IHWTAIDSCVSVVEFLKKNSSLDAERNCGAISDSSPSRNCSAETESNAASLIHFANCVLD 900
Query: 901 VHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLR 960
VHSLKD VVLAIHTGK+YSIVEVV+NTSAES FDGNSD+A DYI FADYF K+YGITL
Sbjct: 901 VHSLKDRVVLAIHTGKIYSIVEVVNNTSAESPFDGNSDKAPPDYITFADYFNKRYGITLS 960
Query: 961 YPQQPMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKG 1020
YPQQP+LRLKQSHNPHNLLVNF DEG TSDKLQSG A +KPQMHVHMPPELV+SMDVPKG
Sbjct: 961 YPQQPLLRLKQSHNPHNLLVNFKDEGVTSDKLQSGSAVRKPQMHVHMPPELVLSMDVPKG 1020
Query: 1021 VWKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERL 1080
V KS+YLLPSLMHR ESLMLASQLRKEINYHSNSLCISSSLI+EALTTLG CESFS ERL
Sbjct: 1021 VLKSSYLLPSLMHRFESLMLASQLRKEINYHSNSLCISSSLILEALTTLGSCESFSFERL 1080
Query: 1081 ELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFD 1140
ELLGDSVLKYAVSCHLFLKFPEKHEGQLSS+RQQVICNATLH LG KCKLQGYIRDSAFD
Sbjct: 1081 ELLGDSVLKYAVSCHLFLKFPEKHEGQLSSRRQQVICNATLHALGTKCKLQGYIRDSAFD 1140
Query: 1141 PRRWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADC 1200
PRRWVAPG T RPVPCKCGVDT EVPLDDKFC+EDPKVVLGKCCDKGHRW+VSKTIADC
Sbjct: 1141 PRRWVAPGHCTLRPVPCKCGVDTAEVPLDDKFCSEDPKVVLGKCCDKGHRWLVSKTIADC 1200
Query: 1201 VEALIGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESK 1260
EALIGAYYV+ GI AALHVIKWLGIDADL V+LVVDAI SASLRSC LK+TEI TLESK
Sbjct: 1201 AEALIGAYYVDGGIIAALHVIKWLGIDADLDVALVVDAIASASLRSCNLKDTEIATLESK 1260
Query: 1261 IGYEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGEL 1320
IGYEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITW+YYQ +SDIDPGEL
Sbjct: 1261 IGYEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWYYYQKYSDIDPGEL 1320
Query: 1321 TDLRSASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGP 1380
TDLRSASVNNE FAQVAVRRNLQQHLQHC GLLI QITEYVKYLSES DAGKSLHGNKGP
Sbjct: 1321 TDLRSASVNNETFAQVAVRRNLQQHLQHCSGLLISQITEYVKYLSESHDAGKSLHGNKGP 1380
Query: 1381 KVLGDMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYF 1440
KVLGDMVESIAGAILIDT+LNLDEVW+IYKPLLTP VTPDKLEL PLRELIELCDSLGYF
Sbjct: 1381 KVLGDMVESIAGAILIDTKLNLDEVWKIYKPLLTPLVTPDKLELPPLRELIELCDSLGYF 1440
Query: 1441 IKDKCTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSG 1500
IKDKCTRKGEI HAELRLQLQDVLL+GEGYERTRKA+RGEAA RLLVQLETRGI YSRSG
Sbjct: 1441 IKDKCTRKGEIFHAELRLQLQDVLLVGEGYERTRKASRGEAAQRLLVQLETRGISYSRSG 1500
Query: 1501 SKKRKQNPNHVGECHLPGSWLRLIAFV----AHFAVIGPVSLKKGGPRMTLFELCKKLQW 1560
SKKRKQNP+ V + S + + VIGP++LKKGGPR TLFELCKKLQW
Sbjct: 1501 SKKRKQNPSCVEAAMVLSSSINCSTDACDPNSETPVIGPINLKKGGPRSTLFELCKKLQW 1560
Query: 1561 PMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLD 1620
PMPTFNTVENKSRV ++ GEGSERR GFNS+ SNIILQIPNAG IEC+GDARADKKSSLD
Sbjct: 1561 PMPTFNTVENKSRVLIDIGEGSERRKGFNSYLSNIILQIPNAGCIECKGDARADKKSSLD 1620
Query: 1621 SASLVMLKELQRQGRVNINDS 1633
SASLVML+EL+R+GRV INDS
Sbjct: 1621 SASLVMLEELRRRGRVVINDS 1641
BLAST of MC08g0468 vs. ExPASy TrEMBL
Match:
A0A6J1BR97 (endoribonuclease Dicer homolog 3a isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004983 PE=3 SV=1)
HSP 1 Score: 3192 bits (8276), Expect = 0.0
Identity = 1615/1661 (97.23%), Postives = 1620/1661 (97.53%), Query Frame = 0
Query: 1 MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV 60
MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV
Sbjct: 1 MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV 60
Query: 61 LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY 120
LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY
Sbjct: 61 LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY 120
Query: 121 GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP 180
GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP
Sbjct: 121 GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP 180
Query: 181 YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE 240
YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE
Sbjct: 181 YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE 240
Query: 241 VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP 300
VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP
Sbjct: 241 VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP 300
Query: 301 LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL 360
LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL
Sbjct: 301 LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL 360
Query: 361 LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII 420
LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII
Sbjct: 361 LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII 420
Query: 421 VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE 480
VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE
Sbjct: 421 VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE 480
Query: 481 EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER 540
EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER
Sbjct: 481 EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER 540
Query: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP 600
SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP
Sbjct: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP 600
Query: 601 KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH 660
KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH
Sbjct: 601 KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH 660
Query: 661 LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD 720
LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD
Sbjct: 661 LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD 720
Query: 721 FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK 780
FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK
Sbjct: 721 FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK 780
Query: 781 CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK 840
CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK
Sbjct: 781 CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK 840
Query: 841 IHWRAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSL 900
IHWRAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSL
Sbjct: 841 IHWRAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSL 900
Query: 901 KDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQ 960
KDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQ
Sbjct: 901 KDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQ 960
Query: 961 PMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKS 1020
PMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKS
Sbjct: 961 PMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKS 1020
Query: 1021 TYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLG 1080
TYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLG
Sbjct: 1021 TYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLG 1080
Query: 1081 DSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRW 1140
DSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRW
Sbjct: 1081 DSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRW 1140
Query: 1141 VAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEAL 1200
VAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEAL
Sbjct: 1141 VAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEAL 1200
Query: 1201 IGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYE 1260
IGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYE
Sbjct: 1201 IGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYE 1260
Query: 1261 FAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLR 1320
FAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLR
Sbjct: 1261 FAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLR 1320
Query: 1321 SASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLG 1380
SASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLG
Sbjct: 1321 SASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLG 1380
Query: 1381 DMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDK 1440
DMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDK
Sbjct: 1381 DMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDK 1440
Query: 1441 CTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKR 1500
CTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKR
Sbjct: 1441 CTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKR 1500
Query: 1501 KQNPNHVGECHLPGSWLRLI------------AFVAHFA----------------VIGPV 1560
KQNPNHV + S + + + H + VIGPV
Sbjct: 1501 KQNPNHVESALVLPSSINCSTEACDPNLETPDSVMVHSSATNCSTEGYSPGMDIPVIGPV 1560
Query: 1561 SLKKGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIP 1620
SLKKGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIP
Sbjct: 1561 SLKKGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIP 1620
Query: 1621 NAGSIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS 1633
NAGSIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS
Sbjct: 1621 NAGSIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS 1661
BLAST of MC08g0468 vs. ExPASy TrEMBL
Match:
A0A6J1BR19 (endoribonuclease Dicer homolog 3a isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004983 PE=3 SV=1)
HSP 1 Score: 2906 bits (7533), Expect = 0.0
Identity = 1471/1517 (96.97%), Postives = 1476/1517 (97.30%), Query Frame = 0
Query: 145 MTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTKIMKDFYHKSDNKPKIFGMTAS 204
MTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTKIMKDFYHKSDNKPKIFGMTAS
Sbjct: 1 MTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTKIMKDFYHKSDNKPKIFGMTAS 60
Query: 205 PVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKSPSME 264
PVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKSPSME
Sbjct: 61 PVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAKSPSME 120
Query: 265 LKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKKRLSSDHLKISYCLDELGLICA 324
LKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKKRLSSDHLKISYCLDELGLICA
Sbjct: 121 LKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKKRLSSDHLKISYCLDELGLICA 180
Query: 325 YEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVIGESFPLGNENVLNFGLDIWKA 384
YEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVIGESFPLGNENVLNFGLDIWKA
Sbjct: 181 YEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVIGESFPLGNENVLNFGLDIWKA 240
Query: 385 VDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAKVIERFVKKVPKLSHFTVSYVT 444
VDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAKVIERFVKKVPKLSHFTVSYVT
Sbjct: 241 VDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAKVIERFVKKVPKLSHFTVSYVT 300
Query: 445 GCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSYV 504
GCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSYV
Sbjct: 301 GCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGLHVPNCSSVVRFDLPKTIRSYV 360
Query: 505 QSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMTDAAMSRDPDACILKPFSIVET 564
QSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMTDAAMSRDPDACILKPFSIVET
Sbjct: 361 QSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMTDAAMSRDPDACILKPFSIVET 420
Query: 565 DCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPIFQLSYGEGSYECRLTLPPTAA 624
DCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPIFQLSYGEGSYECRLTLPPTAA
Sbjct: 421 DCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPIFQLSYGEGSYECRLTLPPTAA 480
Query: 625 FQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRA 684
FQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRA
Sbjct: 481 FQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRA 540
Query: 685 GTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSCSVVYEIYSGFVLLIESKLDDD 744
GTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSCSVVYEIYSGFVLLIESKLDDD
Sbjct: 541 GTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSCSVVYEIYSGFVLLIESKLDDD 600
Query: 745 VGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGK 804
VGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGK
Sbjct: 601 VGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGK 660
Query: 805 REFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVEFLKKNSSLDT 864
REFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVEFLKKNSSLDT
Sbjct: 661 REFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHWRAVDSCVSVVEFLKKNSSLDT 720
Query: 865 ESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTS 924
ESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTS
Sbjct: 721 ESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTS 780
Query: 925 AESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLLVNFNDEGET 984
AESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLLVNFNDEGET
Sbjct: 781 AESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLLVNFNDEGET 840
Query: 985 SDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEI 1044
SDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEI
Sbjct: 841 SDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEI 900
Query: 1045 NYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQL 1104
NYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQL
Sbjct: 901 NYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQL 960
Query: 1105 SSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCGVDTVEVPL 1164
SSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCGVDTVEVPL
Sbjct: 961 SSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCGVDTVEVPL 1020
Query: 1165 DDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDA 1224
DDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDA
Sbjct: 1021 DDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDA 1080
Query: 1225 DLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYC 1284
DLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYC
Sbjct: 1081 DLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYC 1140
Query: 1285 YQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQH 1344
YQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQH
Sbjct: 1141 YQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQH 1200
Query: 1345 CCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMVESIAGAILIDTELNLDEVWRI 1404
CCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMVESIAGAILIDTELNLDEVWRI
Sbjct: 1201 CCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMVESIAGAILIDTELNLDEVWRI 1260
Query: 1405 YKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLIGE 1464
YKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLIGE
Sbjct: 1261 YKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLIGE 1320
Query: 1465 GYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVGECHLPGSWLRLI---- 1524
GYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHV + S +
Sbjct: 1321 GYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVESALVLPSSINCSTEAC 1380
Query: 1525 --------AFVAHFA----------------VIGPVSLKKGGPRMTLFELCKKLQWPMPT 1584
+ + H + VIGPVSLKKGGPRMTLFELCKKLQWPMPT
Sbjct: 1381 DPNLETPDSVMVHSSATNCSTEGYSPGMDIPVIGPVSLKKGGPRMTLFELCKKLQWPMPT 1440
Query: 1585 FNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLDSASL 1633
FNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLDSASL
Sbjct: 1441 FNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLDSASL 1500
BLAST of MC08g0468 vs. ExPASy TrEMBL
Match:
A0A6J1BSN5 (endoribonuclease Dicer homolog 3a isoform X3 OS=Momordica charantia OX=3673 GN=LOC111004983 PE=4 SV=1)
HSP 1 Score: 2822 bits (7316), Expect = 0.0
Identity = 1429/1478 (96.68%), Postives = 1436/1478 (97.16%), Query Frame = 0
Query: 184 IMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEI 243
++ DFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEI
Sbjct: 5 VLLDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEI 64
Query: 244 YVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKK 303
YVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKK
Sbjct: 65 YVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKK 124
Query: 304 RLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVI 363
RLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVI
Sbjct: 125 RLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVI 184
Query: 364 GESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAK 423
GESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAK
Sbjct: 185 GESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAK 244
Query: 424 VIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGL 483
VIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGL
Sbjct: 245 VIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGL 304
Query: 484 HVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMT 543
HVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMT
Sbjct: 305 HVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMT 364
Query: 544 DAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPI 603
DAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPI
Sbjct: 365 DAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPI 424
Query: 604 FQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLP 663
FQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLP
Sbjct: 425 FQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLP 484
Query: 664 LIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSC 723
LIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSC
Sbjct: 485 LIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSC 544
Query: 724 SVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQ 783
SVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQ
Sbjct: 545 SVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQ 604
Query: 784 ELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHW 843
ELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHW
Sbjct: 605 ELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHW 664
Query: 844 RAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDL 903
RAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDL
Sbjct: 665 RAVDSCVSVVEFLKKNSSLDTESNCGALPSRNNSAETGSKAASLIHFANCVLDVHSLKDL 724
Query: 904 VVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPML 963
VVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPML
Sbjct: 725 VVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQQPML 784
Query: 964 RLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYL 1023
RLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYL
Sbjct: 785 RLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKGVWKSTYL 844
Query: 1024 LPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSV 1083
LPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSV
Sbjct: 845 LPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLELLGDSV 904
Query: 1084 LKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAP 1143
LKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAP
Sbjct: 905 LKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPRRWVAP 964
Query: 1144 GQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGA 1203
GQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGA
Sbjct: 965 GQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVEALIGA 1024
Query: 1204 YYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAV 1263
YYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAV
Sbjct: 1025 YYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAV 1084
Query: 1264 KGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSAS 1323
KGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSAS
Sbjct: 1085 KGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSAS 1144
Query: 1324 VNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMV 1383
VNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMV
Sbjct: 1145 VNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKVLGDMV 1204
Query: 1384 ESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTR 1443
ESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTR
Sbjct: 1205 ESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTR 1264
Query: 1444 KGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQN 1503
KGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQN
Sbjct: 1265 KGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQN 1324
Query: 1504 PNHVGECHLPGSWLRLI------------AFVAHFA----------------VIGPVSLK 1563
PNHV + S + + + H + VIGPVSLK
Sbjct: 1325 PNHVESALVLPSSINCSTEACDPNLETPDSVMVHSSATNCSTEGYSPGMDIPVIGPVSLK 1384
Query: 1564 KGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAG 1623
KGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAG
Sbjct: 1385 KGGPRMTLFELCKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAG 1444
Query: 1624 SIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS 1633
SIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS
Sbjct: 1445 SIECEGDARADKKSSLDSASLVMLKELQRQGRVNINDS 1482
BLAST of MC08g0468 vs. ExPASy TrEMBL
Match:
A0A1S3BDT0 (endoribonuclease Dicer homolog 3 OS=Cucumis melo OX=3656 GN=LOC103488578 PE=3 SV=1)
HSP 1 Score: 2792 bits (7238), Expect = 0.0
Identity = 1405/1647 (85.31%), Postives = 1496/1647 (90.83%), Query Frame = 0
Query: 1 MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV 60
MH+ E++NR+GARKR F D+EPS ASD S+F M +GFIPRRYQ EVFEVGMRRNTIAV
Sbjct: 1 MHEPEQENRDGARKRRFCDLEPSVASDQSNFN--MAEGFIPRRYQMEVFEVGMRRNTIAV 60
Query: 61 LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY 120
LETG+GKTMIAVMLIKEIG MKSSG KK IIFLAPTVHLVHQQFNVIK HTDFEVAEYY
Sbjct: 61 LETGSGKTMIAVMLIKEIGKAMKSSGDKKLIIFLAPTVHLVHQQFNVIKDHTDFEVAEYY 120
Query: 121 GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP 180
GAMGVDDW++ CWEKETSE DVLVMTPQILLDALRKA+ LGTVCLMIIDECHRATGNHP
Sbjct: 121 GAMGVDDWNMNCWEKETSERDVLVMTPQILLDALRKAYFRLGTVCLMIIDECHRATGNHP 180
Query: 181 YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE 240
YTKIMK+FYHKSDNKPKIFGMTASPVIRKGVSSS DCECQIADLESI+DSQ+YAIEDKKE
Sbjct: 181 YTKIMKEFYHKSDNKPKIFGMTASPVIRKGVSSSFDCECQIADLESILDSQVYAIEDKKE 240
Query: 241 VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP 300
VEIYVPSAKEICIFYEPAK + EL++KIEALWFKFDA LSNSQ A+Q HYKDVDSKLK
Sbjct: 241 VEIYVPSAKEICIFYEPAKPQTTELRAKIEALWFKFDASLSNSQEAMQGHYKDVDSKLKA 300
Query: 301 LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL 360
LKKRLSSDHLKI+YCLDELG++CAYEAIKVL EN+++ NEE +V+RES LQYK FLEEAL
Sbjct: 301 LKKRLSSDHLKITYCLDELGIMCAYEAIKVLFENISVPNEESDVYRESFLQYKHFLEEAL 360
Query: 361 LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII 420
LVIGES LGNEN+ N G D+WKAVDLGYIS KLFELLQLFESFGVSRQ+LCLIFVERII
Sbjct: 361 LVIGESLSLGNENIPNLGFDLWKAVDLGYISPKLFELLQLFESFGVSRQLLCLIFVERII 420
Query: 421 VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE 480
A VI+R VKKV LSHFTVSYVTGCN+SVGALAPKVQKETLE FCHGKLNLLFSTDVVE
Sbjct: 421 AANVIKRIVKKVENLSHFTVSYVTGCNASVGALAPKVQKETLELFCHGKLNLLFSTDVVE 480
Query: 481 EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER 540
EGLHVPNCS VVRFDLPKT+RSYVQSRGRARQNNSQYILLLERGNLKQR LFDV+RS+R
Sbjct: 481 EGLHVPNCSFVVRFDLPKTVRSYVQSRGRARQNNSQYILLLERGNLKQRALLFDVIRSDR 540
Query: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP 600
SMTDAAMSRD DAC+LKPFS+VETDCYIVE TGASVTADSSVGLIYQYCKKLPGDKYFSP
Sbjct: 541 SMTDAAMSRDSDACVLKPFSLVETDCYIVEATGASVTADSSVGLIYQYCKKLPGDKYFSP 600
Query: 601 KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH 660
KPIFQLSYGEGSYEC+LTLPPTAAFQ+IVGPS+RNSNLSKQLVCLEACKKLH++GALNDH
Sbjct: 601 KPIFQLSYGEGSYECQLTLPPTAAFQTIVGPSTRNSNLSKQLVCLEACKKLHKMGALNDH 660
Query: 661 LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD 720
LLP +EEISE D LKSN S SRAG+TKRKELHGRTAI ALSGTWGE EGA+FQAYKFD
Sbjct: 661 LLPSVEEISESDMGLKSNASMSRAGSTKRKELHGRTAIRALSGTWGEQDEGATFQAYKFD 720
Query: 721 FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK 780
FSCSV+YEIYSGFVLLIESKLDDDV N ELELYL SK VKASVSFGG+VHLDAEQI KAK
Sbjct: 721 FSCSVIYEIYSGFVLLIESKLDDDVSNFELELYLLSKMVKASVSFGGEVHLDAEQITKAK 780
Query: 781 CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK 840
CFQELFFN +FGRLFIGSK TG KR+FLLQKDT LW++SNMYLLLPVDL D S+HDLWK
Sbjct: 781 CFQELFFNALFGRLFIGSKTTGKKRDFLLQKDTNSLWISSNMYLLLPVDLPDDSTHDLWK 840
Query: 841 IHWRAVDSCVSVVEFLKKNSSLDTESNCGA------LPSRNNSAETGSKAASLIHFANCV 900
IHWRA+DSCVSVVEFLKKNSSLD E NCGA PSRNNSAETGS AA++IHFANCV
Sbjct: 841 IHWRAIDSCVSVVEFLKKNSSLDAERNCGAGAISDSWPSRNNSAETGSNAANVIHFANCV 900
Query: 901 LDVHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGIT 960
LDVHSLKD VVLAIHTG++YSIVEVVSNTSAES FDGNSD+ DYI FADYF K+YGI+
Sbjct: 901 LDVHSLKDRVVLAIHTGRIYSIVEVVSNTSAESPFDGNSDKGPPDYITFADYFNKRYGIS 960
Query: 961 LRYPQQPMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVP 1020
L +P+QP+LRLKQSHNPHNLLVNF DEG TSDKL SG A KKPQMHVHMPPELV+SMDVP
Sbjct: 961 LSFPKQPLLRLKQSHNPHNLLVNFKDEGNTSDKLPSGPAFKKPQMHVHMPPELVLSMDVP 1020
Query: 1021 KGVWKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLE 1080
KGV KS+YLLPSLMHR ESLMLASQLRKEINYHSNSL ISSSLI+EALTTLG CE+FSLE
Sbjct: 1021 KGVLKSSYLLPSLMHRFESLMLASQLRKEINYHSNSLSISSSLILEALTTLGSCENFSLE 1080
Query: 1081 RLELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSA 1140
RLELLGDSVLKYAVSCHLFLKFPEKHEGQLSS+RQQVICNATLH LG KC LQGYIRDSA
Sbjct: 1081 RLELLGDSVLKYAVSCHLFLKFPEKHEGQLSSRRQQVICNATLHALGTKCGLQGYIRDSA 1140
Query: 1141 FDPRRWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIA 1200
FDPRRWVAPGQ T RPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIA
Sbjct: 1141 FDPRRWVAPGQCTNRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIA 1200
Query: 1201 DCVEALIGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLE 1260
DC EALIGAY+V+ GI AALHV+KWLGID DL V+LVVDAITSASLRSC L +TEI TLE
Sbjct: 1201 DCAEALIGAYFVDGGIIAALHVMKWLGIDVDLDVALVVDAITSASLRSCPLGDTEIATLE 1260
Query: 1261 SKIGYEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPG 1320
SKIGY+FAVKGLLLEAITHTSDQE+GVNYCYQRLEFLGDSVLDLLITWHYYQ + DIDPG
Sbjct: 1261 SKIGYDFAVKGLLLEAITHTSDQEIGVNYCYQRLEFLGDSVLDLLITWHYYQKYLDIDPG 1320
Query: 1321 ELTDLRSASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNK 1380
ELTDLRSASVNNENFAQVAVRRNLQ HLQHC GLL+ QITEYVKYLSES D GK LHGNK
Sbjct: 1321 ELTDLRSASVNNENFAQVAVRRNLQHHLQHCSGLLMSQITEYVKYLSESHDTGKPLHGNK 1380
Query: 1381 GPKVLGDMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLG 1440
GPKVLGDMVESIAGAILIDT+LNLDEVW+IYKPLLTPFVTPDKLEL PLRELIELCDSLG
Sbjct: 1381 GPKVLGDMVESIAGAILIDTKLNLDEVWKIYKPLLTPFVTPDKLELPPLRELIELCDSLG 1440
Query: 1441 YFIKDKCTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSR 1500
YFIKDKCTRKGE HAELRLQLQD LLIGEGYERTRKA+RGEAAHRLLVQLETRGI YSR
Sbjct: 1441 YFIKDKCTRKGETFHAELRLQLQDALLIGEGYERTRKASRGEAAHRLLVQLETRGISYSR 1500
Query: 1501 SGSKKRKQNPNHVGECHLPGSWLRLIA--------FVAHFAVIGPVSLKKGGPRMTLFEL 1560
S SKKRKQNP+H GE H S L ++ F A+ AVIGP++LKKGGPR TLFEL
Sbjct: 1501 SASKKRKQNPSHDGEYHFCESQLSILPRQAPSRQYFFAYVAVIGPINLKKGGPRSTLFEL 1560
Query: 1561 CKKLQWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARAD 1620
CKKLQWPMPTFNTVENKSRV +EFGEG E+R GFNSF SNI LQIPNAG +ECEG+ARAD
Sbjct: 1561 CKKLQWPMPTFNTVENKSRVQIEFGEGLEKRKGFNSFLSNITLQIPNAGYVECEGEARAD 1620
Query: 1621 KKSSLDSASLVMLKELQRQGRVNINDS 1633
KKSS DSASLVML+ELQ+QGRV INDS
Sbjct: 1621 KKSSFDSASLVMLQELQQQGRVIINDS 1645
BLAST of MC08g0468 vs. ExPASy TrEMBL
Match:
A0A6J1KK54 (endoribonuclease Dicer homolog 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493921 PE=3 SV=1)
HSP 1 Score: 2779 bits (7203), Expect = 0.0
Identity = 1402/1641 (85.44%), Postives = 1497/1641 (91.22%), Query Frame = 0
Query: 1 MHDLEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAV 60
M++ E+N +GARKR+F+DME S SD ++F +PMD+GFIPRRYQ EVFEVGM+RNTIAV
Sbjct: 1 MYNPVEENCDGARKRSFNDMESSVTSDQTNFDKPMDEGFIPRRYQMEVFEVGMKRNTIAV 60
Query: 61 LETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYY 120
LETGAGKTMIAVMLIKEIG MKSSG KK+IIFLAPTVHLVHQQFNVIK TDFEVAEYY
Sbjct: 61 LETGAGKTMIAVMLIKEIGKAMKSSGDKKFIIFLAPTVHLVHQQFNVIKVQTDFEVAEYY 120
Query: 121 GAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHP 180
GAMGVDDW++ CWEKETSE DVLVMTPQILLDALRKA+ LGTVCLMI+DECHRATGNHP
Sbjct: 121 GAMGVDDWNMNCWEKETSERDVLVMTPQILLDALRKAYFRLGTVCLMIMDECHRATGNHP 180
Query: 181 YTKIMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKE 240
YTKIMK+FYHKSDNKPKIFGMTASPVIRKGVSSS DCECQI +LESI+DS+IYAIEDKKE
Sbjct: 181 YTKIMKEFYHKSDNKPKIFGMTASPVIRKGVSSSLDCECQIGELESILDSKIYAIEDKKE 240
Query: 241 VEIYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKP 300
VEIYVPSAKE CIFYEPAKS ++ELK+KIEALW KFD LLSNSQ A+QVHYKDVDSKLK
Sbjct: 241 VEIYVPSAKETCIFYEPAKSLTLELKAKIEALWSKFDTLLSNSQVALQVHYKDVDSKLKA 300
Query: 301 LKKRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEAL 360
L+KRLSSDH KISYCLDELG++CAYEAIKVLLENVNI N EYE+ RESSLQYK FLEEAL
Sbjct: 301 LRKRLSSDHFKISYCLDELGVLCAYEAIKVLLENVNIPNNEYELSRESSLQYKQFLEEAL 360
Query: 361 LVIGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERII 420
VIGES PLG+EN+ NFG D KAVDLGYIS KLFELLQLFESFGVSRQVLCL+FVERII
Sbjct: 361 RVIGESLPLGHENIPNFGFDFQKAVDLGYISPKLFELLQLFESFGVSRQVLCLVFVERII 420
Query: 421 VAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVE 480
A VIERFV+KV LSHFTVSYVTGCN+SVGALAPKVQKETLE F HGK+NLLFSTDVVE
Sbjct: 421 AANVIERFVRKVANLSHFTVSYVTGCNTSVGALAPKVQKETLELFRHGKVNLLFSTDVVE 480
Query: 481 EGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSER 540
EGLHVPNCS VVRFDLPKT+RSYVQSRGRARQ+NSQYILLLERGN+KQR+QLFDV+RS+R
Sbjct: 481 EGLHVPNCSFVVRFDLPKTVRSYVQSRGRARQSNSQYILLLERGNVKQREQLFDVIRSDR 540
Query: 541 SMTDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSP 600
SMT+AAMSRDPDAC+LKPFSI+ETDCYIVE TGASVTADSSVGLI+QYCKKLPGDKYFSP
Sbjct: 541 SMTEAAMSRDPDACMLKPFSIMETDCYIVEATGASVTADSSVGLIHQYCKKLPGDKYFSP 600
Query: 601 KPIFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDH 660
KPIFQLSYGEGSYECRL LPPTAAFQ+IVGPS+RNSNLSKQLVCLEACKKLHQ+GALNDH
Sbjct: 601 KPIFQLSYGEGSYECRLILPPTAAFQTIVGPSTRNSNLSKQLVCLEACKKLHQMGALNDH 660
Query: 661 LLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFD 720
LLP IEEISEFD L+ N STS AGTTKRKELHGRT I ALSGTWGE VEGA+FQAYKFD
Sbjct: 661 LLPSIEEISEFDMGLRCNASTSGAGTTKRKELHGRTTIRALSGTWGEKVEGATFQAYKFD 720
Query: 721 FSCSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAK 780
FSCSV+YE +SGFVLL+ESKLDDDVGN ELELYL SK VKASVSF GQVH+DAEQI KAK
Sbjct: 721 FSCSVIYEHFSGFVLLLESKLDDDVGNFELELYLISKMVKASVSFAGQVHMDAEQIMKAK 780
Query: 781 CFQELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWK 840
CFQELFFNG+FGRL IGSKLTG KR+FLL KDTKPLW+TSNMYLLLPVD DASS DLWK
Sbjct: 781 CFQELFFNGLFGRLLIGSKLTGKKRDFLLLKDTKPLWITSNMYLLLPVDSPDASSDDLWK 840
Query: 841 IHWRAVDSCVSVVEFLKKNSSLDTESNCGAL----PSRNNSAETGSKAASLIHFANCVLD 900
IHWRA+DSCVSVVEFLKKNSSLD E NCGA+ PSRN SAET S AASLIHFANCVLD
Sbjct: 841 IHWRAIDSCVSVVEFLKKNSSLDAERNCGAISDSSPSRNCSAETESNAASLIHFANCVLD 900
Query: 901 VHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLR 960
VHSLKD VVLAIHTGK+YSIVEVV+NTSAES FDGNSD+A DYI FADYF K+YGITL
Sbjct: 901 VHSLKDRVVLAIHTGKIYSIVEVVNNTSAESPFDGNSDKAPPDYITFADYFNKRYGITLS 960
Query: 961 YPQQPMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASKKPQMHVHMPPELVVSMDVPKG 1020
YPQQP+LRLKQSHNPHNLLVNF DEG TSDKLQSG A +KPQMHVHMPPELV+SMDVPKG
Sbjct: 961 YPQQPLLRLKQSHNPHNLLVNFKDEGVTSDKLQSGSAVRKPQMHVHMPPELVLSMDVPKG 1020
Query: 1021 VWKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERL 1080
V KS+YLLPSLMHR ESLMLASQLRKEINYHSNSLCISSSLI+EALTTLG CESFS ERL
Sbjct: 1021 VLKSSYLLPSLMHRFESLMLASQLRKEINYHSNSLCISSSLILEALTTLGSCESFSFERL 1080
Query: 1081 ELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFD 1140
ELLGDSVLKYAVSCHLFLKFPEKHEGQLSS+RQQVICNATLH LG KCKLQGYIRDSAFD
Sbjct: 1081 ELLGDSVLKYAVSCHLFLKFPEKHEGQLSSRRQQVICNATLHALGTKCKLQGYIRDSAFD 1140
Query: 1141 PRRWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADC 1200
PRRWVAPG T RPVPCKCGVDT EVPLDDKFC+EDPKVVLGKCCDKGHRW+VSKTIADC
Sbjct: 1141 PRRWVAPGHGTLRPVPCKCGVDTAEVPLDDKFCSEDPKVVLGKCCDKGHRWLVSKTIADC 1200
Query: 1201 VEALIGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESK 1260
EALIGAYYV+ GI AALHVIKWLGIDADL V+LVVDAITSASLRSC LK+TEI TLESK
Sbjct: 1201 AEALIGAYYVDGGIIAALHVIKWLGIDADLDVALVVDAITSASLRSCNLKDTEIATLESK 1260
Query: 1261 IGYEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGEL 1320
IGYEF+VKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITW+YYQ + DIDPGEL
Sbjct: 1261 IGYEFSVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWYYYQKYPDIDPGEL 1320
Query: 1321 TDLRSASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGP 1380
TDLRSASVNN+ FAQVAVRRNLQQHLQHC GLLI QITEYVKYLSES DAGKSLHGNKGP
Sbjct: 1321 TDLRSASVNNKTFAQVAVRRNLQQHLQHCSGLLISQITEYVKYLSESHDAGKSLHGNKGP 1380
Query: 1381 KVLGDMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYF 1440
KVLGDMVESIAGAILIDT+LNLDEVW+IYKPLLTP VTPDKLEL PLREL ELCDSLGYF
Sbjct: 1381 KVLGDMVESIAGAILIDTKLNLDEVWKIYKPLLTPLVTPDKLELPPLRELNELCDSLGYF 1440
Query: 1441 IKDKCTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSG 1500
IKDKCTRKGEI HAELRLQLQDVLL+GEGYERTRKA+RGEAA RLLVQLETRGI YSRSG
Sbjct: 1441 IKDKCTRKGEIFHAELRLQLQDVLLVGEGYERTRKASRGEAAQRLLVQLETRGISYSRSG 1500
Query: 1501 SKKRKQNPNHVGECHLPGSWLRLIAFV----AHFAVIGPVSLKKGGPRMTLFELCKKLQW 1560
SKKRKQNP+ V + S + + VIGP++LKKGGPR TLFELCKKLQW
Sbjct: 1501 SKKRKQNPSCVEAAMVLSSSINCSTDACDPNSETPVIGPINLKKGGPRSTLFELCKKLQW 1560
Query: 1561 PMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSLD 1620
PMPTFNTVE+KSRV ++ GEGSERR GFNS+ SNIILQIPNAG IEC+GDARADKKSSLD
Sbjct: 1561 PMPTFNTVEHKSRVLIDIGEGSERRKGFNSYLSNIILQIPNAGCIECKGDARADKKSSLD 1620
Query: 1621 SASLVMLKELQRQGRVNINDS 1633
SASLVML+EL++QGRV INDS
Sbjct: 1621 SASLVMLEELRQQGRVVINDS 1641
BLAST of MC08g0468 vs. TAIR 10
Match:
AT3G43920.2 (dicer-like 3 )
HSP 1 Score: 1491.5 bits (3860), Expect = 0.0e+00
Identity = 834/1635 (51.01%), Postives = 1081/1635 (66.12%), Query Frame = 0
Query: 4 LEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAVLET 63
+EE + + KR FS+++ D D Y+ +V+EV RN IAVL T
Sbjct: 10 MEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSN---GSYELKVYEVAKNRNIIAVLGT 69
Query: 64 GAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYYGAM 123
G K+ I LIK +G S K+ IIFLAPTV+LV QQ I+A + +V EY+GA
Sbjct: 70 GIDKSEITKRLIKAMG---SSDTDKRLIIFLAPTVNLVKQQCCEIRALVNLKVEEYFGAK 129
Query: 124 GVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTK 183
GVD W+ + W++E S+HDVLVMTPQILLD LR AFL L VCL+IIDECH TGNHPY K
Sbjct: 130 GVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAK 189
Query: 184 IMKDFYHKSDNKPKIFGMTASPVIRKG-VSSSSDCECQIADLESIMDSQIYAIEDKKEVE 243
+MK+FYH+S +KPKIFG+TAS VIRKG VSS S+ Q+++LE +MDS+I+ E+++ VE
Sbjct: 190 LMKEFYHESTSKPKIFGLTASAVIRKGIVSSPSNYAAQVSELERLMDSKIFNPEEREGVE 249
Query: 244 IYVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLK 303
+ + KE I Y P+ S S+ELK K+E KFDA L Q + + ++D+K + +
Sbjct: 250 KFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQ 309
Query: 304 KRLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLV 363
KRLS D+ +I +CLD LGLICA+ A +V LE ++ T EE E ++E S+ K FLE+ L
Sbjct: 310 KRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILST 369
Query: 364 IGESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVA 423
IG P ++++++ + AV G++S KL EL L +SF +Q CLI VERII A
Sbjct: 370 IGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITA 429
Query: 424 KVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEG 483
KVIERFVKK L++ V Y+T N S A K+Q E + F HGK+NLLF TDVVEEG
Sbjct: 430 KVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEG 489
Query: 484 LHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSM 543
VP+CS +V FDLPKT+ SY QS+ A+Q+NS+ I+ LERGN KQRD L D+MR E
Sbjct: 490 FQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRRE--- 549
Query: 544 TDAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKP 603
+ +DP+A LK + + V+ G+ V DS++ + +
Sbjct: 550 ---VLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEE--------------- 609
Query: 604 IFQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLL 663
A+ Q++ P SRN L CKKL L+++LL
Sbjct: 610 -------------------AASTQTMSDPPSRNEQ-------LPPCKKLR----LDNNLL 669
Query: 664 PLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFS 723
E + KS S+S AG+ KRKELHG T ALSGTWGE ++GA+FQAYKFDF
Sbjct: 670 Q--SNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFC 729
Query: 724 CSVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCF 783
C++ E+YS F LL+ES L +DVG +E++LYL K VKASVS GQ+ L E++ KAK F
Sbjct: 730 CNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYF 789
Query: 784 QELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIH 843
Q+ FFNGMFG+LF+GSK G KREFLLQ DT LW + M+LLLPV+ D +S I
Sbjct: 790 QQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSA--TID 849
Query: 844 WRAVDSCVSVVEFLKKNSSLDTE----SNCGALPSRNNSAETGSKAASLIHFANCVLDVH 903
W A++SC S+VEFLKKNS LD + C + + + +LIHFAN D +
Sbjct: 850 WSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKN 909
Query: 904 SLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYP 963
SL++LVV+AIHTG++YSIVE VS++SA S F+ + A S Y +A+YF KKYGI L +P
Sbjct: 910 SLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVD---ASSGYATYAEYFNKKYGIVLAHP 969
Query: 964 QQPMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASK-KPQMHVHMPPELVVSMDVPKGV 1023
QP+++LKQSH+ HNLLV+FN+E + ++G+ K KP +H H+PPEL+ +DVP+ V
Sbjct: 970 NQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAV 1029
Query: 1024 WKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLE 1083
KS YLLPS+MHRLESLMLASQLR+EI+ ++ ISS+ I+EA+TTL C ESFS+ERLE
Sbjct: 1030 LKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLE 1089
Query: 1084 LLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDP 1143
LLGDSVLKY SCHLFLK+P+K EGQLS QRQ +I N+ LHRL KLQGYIR+ AF+P
Sbjct: 1090 LLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEP 1149
Query: 1144 RRWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCV 1203
RRW APGQ + PVPCKCG+DT EVPLD KF TE+ + +GK CD GHRWVVSK+++DC
Sbjct: 1150 RRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCA 1209
Query: 1204 EALIGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKI 1263
EALIGAYYV+ G+ A+LH++KWLGID D +LVV+AI SLR I KE E+ LE KI
Sbjct: 1210 EALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKI 1269
Query: 1264 GYEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELT 1323
+EF+ K LL EAITH+S +E +Y Y+RLEFLGDSVLD LIT H + T+ PGE+T
Sbjct: 1270 QHEFSAKFLLKEAITHSSLRE---SYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMT 1329
Query: 1324 DLRSASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPK 1383
DLRSA VNNENFAQVAV+ NL HLQ C +L QI +Y+ + + G+S+ +GPK
Sbjct: 1330 DLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPK 1389
Query: 1384 VLGDMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFI 1443
LGD+VESIAGA+LIDT L+LD+VWR+++PLL+P VTPDKL+L P REL ELCDSLGYF
Sbjct: 1390 ALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFF 1449
Query: 1444 KDKCTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGS 1503
+ KC+ G A ++LQL DVLL G+G E+T K A G+AA LL QLE R I SR S
Sbjct: 1450 RVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNI--SRKTS 1509
Query: 1504 KKRKQNP-------NHVGECHLPGSWLRLIAFVAHFAVIGPVSLKKGGPRMTLFELCKKL 1563
Q+ NH L + + V F IGP+++KKGGPR TL E CKK
Sbjct: 1510 LGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPF--IGPINMKKGGPRGTLHEFCKKH 1569
Query: 1564 QWPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSS 1623
WPMPTF+T E KSR P EF +G E+RT F+SF S I L+IPN ++ G+AR DKKSS
Sbjct: 1570 LWPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSS 1573
Query: 1624 LDSASLVMLKELQRQ 1626
DSA + +L EL+R+
Sbjct: 1630 FDSAVVELLYELERR 1573
BLAST of MC08g0468 vs. TAIR 10
Match:
AT3G43920.3 (dicer-like 3 )
HSP 1 Score: 1481.8 bits (3835), Expect = 0.0e+00
Identity = 829/1634 (50.73%), Postives = 1075/1634 (65.79%), Query Frame = 0
Query: 4 LEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAVLET 63
+EE + + KR FS+++ D D Y+ +V+EV RN IAVL T
Sbjct: 10 MEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSN---GSYELKVYEVAKNRNIIAVLGT 69
Query: 64 GAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYYGAM 123
G K+ I LIK +G S K+ IIFLAPTV+LV QQ I+A + +V EY+GA
Sbjct: 70 GIDKSEITKRLIKAMG---SSDTDKRLIIFLAPTVNLVKQQCCEIRALVNLKVEEYFGAK 129
Query: 124 GVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTK 183
GVD W+ + W++E S+HDVLVMTPQILLD LR AFL L VCL+IIDECH TGNHPY K
Sbjct: 130 GVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAK 189
Query: 184 IMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEI 243
+MK+FYH+S +KPKIFG+TAS VIRK Q+++LE +MDS+I+ E+++ VE
Sbjct: 190 LMKEFYHESTSKPKIFGLTASAVIRK---------AQVSELERLMDSKIFNPEEREGVEK 249
Query: 244 YVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKK 303
+ + KE I Y P+ S S+ELK K+E KFDA L Q + + ++D+K + +K
Sbjct: 250 FATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQK 309
Query: 304 RLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVI 363
RLS D+ +I +CLD LGLICA+ A +V LE ++ T EE E ++E S+ K FLE+ L I
Sbjct: 310 RLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTI 369
Query: 364 GESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAK 423
G P ++++++ + AV G++S KL EL L +SF +Q CLI VERII AK
Sbjct: 370 GVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAK 429
Query: 424 VIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGL 483
VIERFVKK L++ V Y+T N S A K+Q E + F HGK+NLLF TDVVEEG
Sbjct: 430 VIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGF 489
Query: 484 HVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMT 543
VP+CS +V FDLPKT+ SY QS+ A+Q+NS+ I+ LERGN KQRD L D+MR E
Sbjct: 490 QVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRRE---- 549
Query: 544 DAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPI 603
+ +DP+A LK + + V+ G+ V DS++ + +
Sbjct: 550 --VLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEE---------------- 609
Query: 604 FQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLP 663
A+ Q++ P SRN L CKKL L+++LL
Sbjct: 610 ------------------AASTQTMSDPPSRNEQ-------LPPCKKLR----LDNNLLQ 669
Query: 664 LIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSC 723
E + KS S+S AG+ KRKELHG T ALSGTWGE ++GA+FQAYKFDF C
Sbjct: 670 --SNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCC 729
Query: 724 SVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQ 783
++ E+YS F LL+ES L +DVG +E++LYL K VKASVS GQ+ L E++ KAK FQ
Sbjct: 730 NISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQ 789
Query: 784 ELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHW 843
+ FFNGMFG+LF+GSK G KREFLLQ DT LW + M+LLLPV+ D +S I W
Sbjct: 790 QFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSA--TIDW 849
Query: 844 RAVDSCVSVVEFLKKNSSLDTE----SNCGALPSRNNSAETGSKAASLIHFANCVLDVHS 903
A++SC S+VEFLKKNS LD + C + + + +LIHFAN D +S
Sbjct: 850 SAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNS 909
Query: 904 LKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQ 963
L++LVV+AIHTG++YSIVE VS++SA S F+ + A S Y +A+YF KKYGI L +P
Sbjct: 910 LEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVD---ASSGYATYAEYFNKKYGIVLAHPN 969
Query: 964 QPMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASK-KPQMHVHMPPELVVSMDVPKGVW 1023
QP+++LKQSH+ HNLLV+FN+E + ++G+ K KP +H H+PPEL+ +DVP+ V
Sbjct: 970 QPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVL 1029
Query: 1024 KSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLEL 1083
KS YLLPS+MHRLESLMLASQLR+EI+ ++ ISS+ I+EA+TTL C ESFS+ERLEL
Sbjct: 1030 KSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLEL 1089
Query: 1084 LGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPR 1143
LGDSVLKY SCHLFLK+P+K EGQLS QRQ +I N+ LHRL KLQGYIR+ AF+PR
Sbjct: 1090 LGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPR 1149
Query: 1144 RWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVE 1203
RW APGQ + PVPCKCG+DT EVPLD KF TE+ + +GK CD GHRWVVSK+++DC E
Sbjct: 1150 RWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAE 1209
Query: 1204 ALIGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIG 1263
ALIGAYYV+ G+ A+LH++KWLGID D +LVV+AI SLR I KE E+ LE KI
Sbjct: 1210 ALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQ 1269
Query: 1264 YEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTD 1323
+EF+ K LL EAITH+S +E +Y Y+RLEFLGDSVLD LIT H + T+ PGE+TD
Sbjct: 1270 HEFSAKFLLKEAITHSSLRE---SYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTD 1329
Query: 1324 LRSASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKV 1383
LRSA VNNENFAQVAV+ NL HLQ C +L QI +Y+ + + G+S+ +GPK
Sbjct: 1330 LRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKA 1389
Query: 1384 LGDMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIK 1443
LGD+VESIAGA+LIDT L+LD+VWR+++PLL+P VTPDKL+L P REL ELCDSLGYF +
Sbjct: 1390 LGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFR 1449
Query: 1444 DKCTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSK 1503
KC+ G A ++LQL DVLL G+G E+T K A G+AA LL QLE R I SR S
Sbjct: 1450 VKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNI--SRKTSL 1509
Query: 1504 KRKQNP-------NHVGECHLPGSWLRLIAFVAHFAVIGPVSLKKGGPRMTLFELCKKLQ 1563
Q+ NH L + + V F IGP+++KKGGPR TL E CKK
Sbjct: 1510 GDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPF--IGPINMKKGGPRGTLHEFCKKHL 1563
Query: 1564 WPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSL 1623
WPMPTF+T E KSR P EF +G E+RT F+SF S I L+IPN ++ G+AR DKKSS
Sbjct: 1570 WPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSSF 1563
Query: 1624 DSASLVMLKELQRQ 1626
DSA + +L EL+R+
Sbjct: 1630 DSAVVELLYELERR 1563
BLAST of MC08g0468 vs. TAIR 10
Match:
AT3G43920.1 (dicer-like 3 )
HSP 1 Score: 1409.8 bits (3648), Expect = 0.0e+00
Identity = 804/1634 (49.20%), Postives = 1041/1634 (63.71%), Query Frame = 0
Query: 4 LEEQNRNGARKRNFSDMEPSFASDFSDFKQPMDKGFIPRRYQTEVFEVGMRRNTIAVLET 63
+EE + + KR FS+++ D D Y+ +V+EV RN IAVL T
Sbjct: 10 MEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSN---GSYELKVYEVAKNRNIIAVLGT 69
Query: 64 GAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLVHQQFNVIKAHTDFEVAEYYGAM 123
G K+ I LIK +G S K+ IIFLAPTV+L Q I+A + +V EY+GA
Sbjct: 70 GIDKSEITKRLIKAMG---SSDTDKRLIIFLAPTVNL---QCCEIRALVNLKVEEYFGAK 129
Query: 124 GVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSLGTVCLMIIDECHRATGNHPYTK 183
GVD W+ + W++E S+HDVLVMTPQILLD LR AFL L VCL+IIDECH TGNHPY K
Sbjct: 130 GVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAK 189
Query: 184 IMKDFYHKSDNKPKIFGMTASPVIRKGVSSSSDCECQIADLESIMDSQIYAIEDKKEVEI 243
+MK I+ E+++ VE
Sbjct: 190 LMK---------------------------------------------IFNPEEREGVEK 249
Query: 244 YVPSAKEICIFYEPAKSPSMELKSKIEALWFKFDALLSNSQGAVQVHYKDVDSKLKPLKK 303
+ + KE I Y P+ S S+ELK K+E KFDA L Q + + ++D+K + +K
Sbjct: 250 FATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQK 309
Query: 304 RLSSDHLKISYCLDELGLICAYEAIKVLLENVNITNEEYEVFRESSLQYKCFLEEALLVI 363
RLS D+ +I +CLD LGLICA+ A +V LE ++ T EE E ++E S+ K FLE+ L I
Sbjct: 310 RLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTI 369
Query: 364 GESFPLGNENVLNFGLDIWKAVDLGYISSKLFELLQLFESFGVSRQVLCLIFVERIIVAK 423
G P ++++++ + AV G++S KL EL L +SF +Q CLI VERII AK
Sbjct: 370 GVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAK 429
Query: 424 VIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLFSTDVVEEGL 483
VIERFVKK L++ V Y+T N S A K+Q E + F HGK+NLLF TDVVEEG
Sbjct: 430 VIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGF 489
Query: 484 HVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFDVMRSERSMT 543
VP+CS +V FDLPKT+ SY QS+ A+Q+NS+ I+ LERGN KQRD L D+MR E
Sbjct: 490 QVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRRE---- 549
Query: 544 DAAMSRDPDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYCKKLPGDKYFSPKPI 603
+ +DP+A LK + + V+ G+ V DS++ + +
Sbjct: 550 --VLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEE---------------- 609
Query: 604 FQLSYGEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVCLEACKKLHQLGALNDHLLP 663
A+ Q++ P SRN L CKKL L+++LL
Sbjct: 610 ------------------AASTQTMSDPPSRNEQ-------LPPCKKLR----LDNNLLQ 669
Query: 664 LIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGTWGELVEGASFQAYKFDFSC 723
E + KS S+S AG+ KRKELHG T ALSGTWGE ++GA+FQAYKFDF C
Sbjct: 670 --SNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCC 729
Query: 724 SVVYEIYSGFVLLIESKLDDDVGNIELELYLRSKTVKASVSFGGQVHLDAEQIRKAKCFQ 783
++ E+YS F LL+ES L +DVG +E++LYL K VKASVS GQ+ L E++ KAK FQ
Sbjct: 730 NISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQ 789
Query: 784 ELFFNGMFGRLFIGSKLTGGKREFLLQKDTKPLWVTSNMYLLLPVDLTDASSHDLWKIHW 843
+ FFNGMFG+LF+GSK G KREFLLQ DT LW + M+LLLPV+ D +S I W
Sbjct: 790 QFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSA--TIDW 849
Query: 844 RAVDSCVSVVEFLKKNSSLDTE----SNCGALPSRNNSAETGSKAASLIHFANCVLDVHS 903
A++SC S+VEFLKKNS LD + C + + + +LIHFAN D +S
Sbjct: 850 SAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNS 909
Query: 904 LKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSDRAQSDYINFADYFKKKYGITLRYPQ 963
L++LVV+AIHTG++YSIVE VS++SA S F+ + A S Y +A+YF KKYGI L +P
Sbjct: 910 LEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVD---ASSGYATYAEYFNKKYGIVLAHPN 969
Query: 964 QPMLRLKQSHNPHNLLVNFNDEGETSDKLQSGHASK-KPQMHVHMPPELVVSMDVPKGVW 1023
QP+++LKQSH+ HNLLV+FN+E + ++G+ K KP +H H+PPEL+ +DVP+ V
Sbjct: 970 QPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVL 1029
Query: 1024 KSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCISSSLIVEALTTLGCCESFSLERLEL 1083
KS YLLPS+MHRLESLMLASQLR+EI+ ++ ISS+ I+EA+TTL C ESFS+ERLEL
Sbjct: 1030 KSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLEL 1089
Query: 1084 LGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICNATLHRLGIKCKLQGYIRDSAFDPR 1143
LGDSVLKY SCHLFLK+P+K EGQLS QRQ +I N+ LHRL KLQGYIR+ AF+PR
Sbjct: 1090 LGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPR 1149
Query: 1144 RWVAPGQRTTRPVPCKCGVDTVEVPLDDKFCTEDPKVVLGKCCDKGHRWVVSKTIADCVE 1203
RW APGQ + PVPCKCG+DT EVPLD KF TE+ + +GK CD GHRWVVSK+++DC E
Sbjct: 1150 RWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAE 1209
Query: 1204 ALIGAYYVNAGIDAALHVIKWLGIDADLGVSLVVDAITSASLRSCILKETEIETLESKIG 1263
ALIGAYYV+ G+ A+LH++KWLGID D +LVV+AI SLR I KE E+ LE KI
Sbjct: 1210 ALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQ 1269
Query: 1264 YEFAVKGLLLEAITHTSDQELGVNYCYQRLEFLGDSVLDLLITWHYYQTHSDIDPGELTD 1323
+EF+ K LL EAITH+S +E +Y Y+RLEFLGDSVLD LIT H + T+ PGE+TD
Sbjct: 1270 HEFSAKFLLKEAITHSSLRE---SYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTD 1329
Query: 1324 LRSASVNNENFAQVAVRRNLQQHLQHCCGLLIGQITEYVKYLSESLDAGKSLHGNKGPKV 1383
LRSA VNNENFAQVAV+ NL HLQ C +L QI +Y+ + + G+S+ +GPK
Sbjct: 1330 LRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKA 1389
Query: 1384 LGDMVESIAGAILIDTELNLDEVWRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIK 1443
LGD+VESIAGA+LIDT L+LD+VWR+++PLL+P VTPDKL+L P REL ELCDSLGYF +
Sbjct: 1390 LGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFR 1449
Query: 1444 DKCTRKGEIIHAELRLQLQDVLLIGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSK 1503
KC+ G A ++LQL DVLL G+G E+T K A G+AA LL QLE R I SR S
Sbjct: 1450 VKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNI--SRKTSL 1509
Query: 1504 KRKQNP-------NHVGECHLPGSWLRLIAFVAHFAVIGPVSLKKGGPRMTLFELCKKLQ 1563
Q+ NH L + + V F IGP+++KKGGPR TL E CKK
Sbjct: 1510 GDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPF--IGPINMKKGGPRGTLHEFCKKHL 1524
Query: 1564 WPMPTFNTVENKSRVPMEFGEGSERRTGFNSFWSNIILQIPNAGSIECEGDARADKKSSL 1623
WPMPTF+T E KSR P EF +G E+RT F+SF S I L+IPN ++ G+AR DKKSS
Sbjct: 1570 WPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSSF 1524
Query: 1624 DSASLVMLKELQRQ 1626
DSA + +L EL+R+
Sbjct: 1630 DSAVVELLYELERR 1524
BLAST of MC08g0468 vs. TAIR 10
Match:
AT1G01040.1 (dicer-like 1 )
HSP 1 Score: 784.6 bits (2025), Expect = 1.5e-226
Identity = 578/1727 (33.47%), Postives = 862/1727 (49.91%), Query Frame = 0
Query: 42 RRYQTEVFEVGMRRNTIAVLETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLV 101
RRYQ +V E +NTIA LETGAGKT+IA++LIK + + S K +FL P V LV
Sbjct: 250 RRYQLDVLEQAKAKNTIAFLETGAGKTLIAILLIKSVHKDLMSQNRKMLSVFLVPKVPLV 309
Query: 102 HQQFNVIKAHTDFEVAEYYGAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSL 161
+QQ VI+ T F+V Y G MG D W + W++E VLVMT QILL+ LR + + +
Sbjct: 310 YQQAEVIRNQTCFQVGHYCGEMGQDFWDSRRWQREFESKQVLVMTAQILLNILRHSIIRM 369
Query: 162 GTVCLMIIDECHRATGNHPYTKIMKDFYHKS--DNKPKIFGMTASPVIRKGVSSSSDCEC 221
T+ L+I+DECH A HPY+ +M +FYH + D +P IFGMTASPV KGVSS DC
Sbjct: 370 ETIDLLILDECHHAVKKHPYSLVMSEFYHTTPKDKRPAIFGMTASPVNLKGVSSQVDCAI 429
Query: 222 QIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAK---SPSMELKSKIEALWFKF 281
+I +LE+ +DS + I+D+KE+E +VP EI + Y+ A S +K I A+
Sbjct: 430 KIRNLETKLDSTVCTIKDRKELEKHVPMPSEIVVEYDKAATMWSLHETIKQMIAAVEEAA 489
Query: 282 DALLSNSQ----GAVQVHYKDVDSKLKPLKKRLSSDHL--------KISYCLDELGLICA 341
A S+ GA KD ++ + +R SD I+Y L ELG CA
Sbjct: 490 QASSRKSKWQFMGARDAGAKDELRQVYGVSERTESDGAANLIHKLRAINYTLAELGQWCA 549
Query: 342 Y---EAIKVLLENVNITNEEYEV-FRESSLQYKCFLEEALLVIG---------------- 401
Y ++ L++ N + +V F+ES L L + L+ G
Sbjct: 550 YKVGQSFLSALQSDERVNFQVDVKFQESYLSEVVSLLQCELLEGAAAEKVAAEVGKPENG 609
Query: 402 ------ESFPLGNENVLNFG--LD--IWKAVDLGYISSKLFELLQLFESFGVSRQVLCLI 461
E L ++ V++ G +D I AV G ++ K+ L++L + + ++
Sbjct: 610 NAHDEMEEGELPDDPVVSGGEHVDEVIGAAVADGKVTPKVQSLIKLLLKYQHTADFRAIV 669
Query: 462 FVERIIVAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLF 521
FVER++ A V+ + ++P LS + + G N+S + ++Q +T+ F G + LL
Sbjct: 670 FVERVVAALVLPKVFAELPSLSFIRCASMIGHNNSQEMKSSQMQ-DTISKFRDGHVTLLV 729
Query: 522 STDVVEEGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFD 581
+T V EEGL + C+ V+RFDL KT+ +Y+QSRGRAR+ S YIL++ERGN+ L +
Sbjct: 730 ATSVAEEGLDIRQCNVVMRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNVSHAAFLRN 789
Query: 582 VMRSERSMTDAAMSRD-----PDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYC 641
SE ++ A+ R D L V Y VE TGA V+ +S+VGL++ YC
Sbjct: 790 ARNSEETLRKEAIERTDLSHLKDTSRLISIDAVPGTVYKVEATGAMVSLNSAVGLVHFYC 849
Query: 642 KKLPGDKYFSPKPIFQLSY-----GEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVC 701
+LPGD+Y +P F + G Y CRL LP A F+ + GP + L++Q VC
Sbjct: 850 SQLPGDRYAILRPEFSMEKHEKPGGHTEYSCRLQLPCNAPFEILEGPVCSSMRLAQQAVC 909
Query: 702 LEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGT 761
L ACKKLH++GA D LLP + + A + + GT + +E + L G
Sbjct: 910 LAACKKLHEMGAFTDMLLPDKGSGQDAEKADQDDEGEPVPGTARHREFYPEGVADVLKGE 969
Query: 762 W----GELVEGAS-FQAYKFDFSC-------SVVYEIYSGFVLLIESKLDDDVGNIELEL 821
W E+ E + F Y ++ C S F +L ++LD +V ++ ++L
Sbjct: 970 WVSSGKEVCESSKLFHLYMYNVRCVDFGSSKDPFLSEVSEFAILFGNELDAEVLSMSMDL 1029
Query: 822 YL-RSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGKREFLLQK 881
Y+ R+ KAS++F G + + Q+ K F + +L
Sbjct: 1030 YVARAMITKASLAFKGSLDITENQLSSLKKFHVRLMS------------------IVLDV 1089
Query: 882 DTKPL---WVTSNMYLLLPVDLTDASSHDLWK-IHWRAVDSCVSVVEF------------ 941
D +P W + YL +PV TD +S + K I+W V+ +
Sbjct: 1090 DVEPSTTPWDPAKAYLFVPV--TDNTSMEPIKGINWELVEKITKTTAWDNPLQRARPDVY 1149
Query: 942 -------------------LKKN-----------------SSLDTESNCGALPSRN---N 1001
L+ N +S D G LP R+
Sbjct: 1150 LGTNERTLGGDRREYGFGKLRHNIVFGQKSHPTYGIRGAVASFDVVRASGLLPVRDAFEK 1209
Query: 1002 SAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSD-R 1061
E L+ C++ L +V A H+GK + + + + SAE++F
Sbjct: 1210 EVEEDLSKGKLMMADGCMV-AEDLIGKIVTAAHSGKRFYVDSICYDMSAETSFPRKEGYL 1269
Query: 1062 AQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLL-VNFNDEGETSDKLQSGHAS 1121
+Y +ADY+K+KYG+ L QQP+++ + NLL F GE+ L +
Sbjct: 1270 GPLEYNTYADYYKQKYGVDLNCKQQPLIKGRGVSYCKNLLSPRFEQSGESETVLDKTY-- 1329
Query: 1122 KKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCIS 1181
+V +PPEL V + + + LPS+M R+ES++LA QL+ I+Y I
Sbjct: 1330 -----YVFLPPELCVVHPLSGSLIRGAQRLPSIMRRVESMLLAVQLKNLISYP-----IP 1389
Query: 1182 SSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVICN 1241
+S I+EALT C E+F ER ELLGD+ LK+ VS LFLK+P+KHEGQL+ RQQ++ N
Sbjct: 1390 TSKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVSN 1449
Query: 1242 ATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCG----VDTVEVPLDDKFCT 1301
L++ + LQ YI+ F P RW APG K G D + P+ ++
Sbjct: 1450 MVLYQFALVKGLQSYIQADRFAPSRWSAPGVPPVFDEDTKDGGSSFFDEEQKPVSEENSD 1509
Query: 1302 --EDPKVVLGKCCD--KGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDADL 1361
ED ++ G+ +R + SKT+AD VEALIG YYV G AA H++KW+GI +
Sbjct: 1510 VFEDGEMEDGELEGDLSSYRVLSSKTLADVVEALIGVYYVEGGKIAANHLMKWIGIHVED 1569
Query: 1362 GVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYCYQ 1421
V + + ++ +LK + LE + YEF KGLL+EAITH S GV+ CYQ
Sbjct: 1570 DPDEVDGTLKNVNVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVS-CYQ 1629
Query: 1422 RLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQHCC 1481
RLEF+GD+VLD LIT H + T++ + PG LTDLR+A+VNNENFA+VAV+ L +L+H
Sbjct: 1630 RLEFVGDAVLDHLITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGS 1689
Query: 1482 GLLIGQITEYVKYL-SESLDAGKSLHG---NKGPKVLGDMVESIAGAILIDTELNLDEVW 1541
L QI E+VK + +ES G + G K PKVLGD+VESIAGAI +D+ + W
Sbjct: 1690 SALEKQIREFVKEVQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAW 1749
Query: 1542 RIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLLI 1601
++++PLL P VTP+ L + P+REL E C ++ K +R G E+ + D + +
Sbjct: 1750 KVFQPLLQPMVTPETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFI---DGVQV 1809
Query: 1602 GEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVGECHLPGSWLRLIAF 1625
G +K A+ AA L L+ + I + SK++ N + GE
Sbjct: 1810 GVAQNPQKKMAQKLAARNALAALKEKEI----AESKEKHINNGNAGEDQGENE------- 1869
BLAST of MC08g0468 vs. TAIR 10
Match:
AT1G01040.2 (dicer-like 1 )
HSP 1 Score: 780.4 bits (2014), Expect = 2.8e-225
Identity = 578/1728 (33.45%), Postives = 862/1728 (49.88%), Query Frame = 0
Query: 42 RRYQTEVFEVGMRRNTIAVLETGAGKTMIAVMLIKEIGLTMKSSGSKKYIIFLAPTVHLV 101
RRYQ +V E +NTIA LETGAGKT+IA++LIK + + S K +FL P V LV
Sbjct: 250 RRYQLDVLEQAKAKNTIAFLETGAGKTLIAILLIKSVHKDLMSQNRKMLSVFLVPKVPLV 309
Query: 102 HQQFNVIKAHTDFEVAEYYGAMGVDDWSLKCWEKETSEHDVLVMTPQILLDALRKAFLSL 161
+QQ VI+ T F+V Y G MG D W + W++E VLVMT QILL+ LR + + +
Sbjct: 310 YQQAEVIRNQTCFQVGHYCGEMGQDFWDSRRWQREFESKQVLVMTAQILLNILRHSIIRM 369
Query: 162 GTVCLMIIDECHRATGNHPYTKIMKDFYHKS--DNKPKIFGMTASPVIRKGVSSSSDCEC 221
T+ L+I+DECH A HPY+ +M +FYH + D +P IFGMTASPV KGVSS DC
Sbjct: 370 ETIDLLILDECHHAVKKHPYSLVMSEFYHTTPKDKRPAIFGMTASPVNLKGVSSQVDCAI 429
Query: 222 QIADLESIMDSQIYAIEDKKEVEIYVPSAKEICIFYEPAK---SPSMELKSKIEALWFKF 281
+I +LE+ +DS + I+D+KE+E +VP EI + Y+ A S +K I A+
Sbjct: 430 KIRNLETKLDSTVCTIKDRKELEKHVPMPSEIVVEYDKAATMWSLHETIKQMIAAVEEAA 489
Query: 282 DALLSNSQ----GAVQVHYKDVDSKLKPLKKRLSSDHL--------KISYCLDELGLICA 341
A S+ GA KD ++ + +R SD I+Y L ELG CA
Sbjct: 490 QASSRKSKWQFMGARDAGAKDELRQVYGVSERTESDGAANLIHKLRAINYTLAELGQWCA 549
Query: 342 Y---EAIKVLLENVNITNEEYEV-FRESSLQYKCFLEEALLVIG---------------- 401
Y ++ L++ N + +V F+ES L L + L+ G
Sbjct: 550 YKVGQSFLSALQSDERVNFQVDVKFQESYLSEVVSLLQCELLEGAAAEKVAAEVGKPENG 609
Query: 402 ------ESFPLGNENVLNFG--LD--IWKAVDLGYISSKLFELLQLFESFGVSRQVLCLI 461
E L ++ V++ G +D I AV G ++ K+ L++L + + ++
Sbjct: 610 NAHDEMEEGELPDDPVVSGGEHVDEVIGAAVADGKVTPKVQSLIKLLLKYQHTADFRAIV 669
Query: 462 FVERIIVAKVIERFVKKVPKLSHFTVSYVTGCNSSVGALAPKVQKETLESFCHGKLNLLF 521
FVER++ A V+ + ++P LS + + G N+S + ++Q +T+ F G + LL
Sbjct: 670 FVERVVAALVLPKVFAELPSLSFIRCASMIGHNNSQEMKSSQMQ-DTISKFRDGHVTLLV 729
Query: 522 STDVVEEGLHVPNCSSVVRFDLPKTIRSYVQSRGRARQNNSQYILLLERGNLKQRDQLFD 581
+T V EEGL + C+ V+RFDL KT+ +Y+QSRGRAR+ S YIL++ERGN+ L +
Sbjct: 730 ATSVAEEGLDIRQCNVVMRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNVSHAAFLRN 789
Query: 582 VMRSERSMTDAAMSRD-----PDACILKPFSIVETDCYIVETTGASVTADSSVGLIYQYC 641
SE ++ A+ R D L V Y VE TGA V+ +S+VGL++ YC
Sbjct: 790 ARNSEETLRKEAIERTDLSHLKDTSRLISIDAVPGTVYKVEATGAMVSLNSAVGLVHFYC 849
Query: 642 KKLPGDKYFSPKPIFQLSY-----GEGSYECRLTLPPTAAFQSIVGPSSRNSNLSKQLVC 701
+LPGD+Y +P F + G Y CRL LP A F+ + GP + L++Q VC
Sbjct: 850 SQLPGDRYAILRPEFSMEKHEKPGGHTEYSCRLQLPCNAPFEILEGPVCSSMRLAQQAVC 909
Query: 702 LEACKKLHQLGALNDHLLPLIEEISEFDTALKSNVSTSRAGTTKRKELHGRTAIGALSGT 761
L ACKKLH++GA D LLP + + A + + GT + +E + L G
Sbjct: 910 LAACKKLHEMGAFTDMLLPDKGSGQDAEKADQDDEGEPVPGTARHREFYPEGVADVLKGE 969
Query: 762 W----GELVEGAS-FQAYKFDFSC-------SVVYEIYSGFVLLIESKLDDDVG-NIELE 821
W E+ E + F Y ++ C S F +L ++LD +V ++ ++
Sbjct: 970 WVSSGKEVCESSKLFHLYMYNVRCVDFGSSKDPFLSEVSEFAILFGNELDAEVVLSMSMD 1029
Query: 822 LYL-RSKTVKASVSFGGQVHLDAEQIRKAKCFQELFFNGMFGRLFIGSKLTGGKREFLLQ 881
LY+ R+ KAS++F G + + Q+ K F + +L
Sbjct: 1030 LYVARAMITKASLAFKGSLDITENQLSSLKKFHVRLMS------------------IVLD 1089
Query: 882 KDTKPL---WVTSNMYLLLPVDLTDASSHDLWK-IHWRAVDSCVSVVEF----------- 941
D +P W + YL +PV TD +S + K I+W V+ +
Sbjct: 1090 VDVEPSTTPWDPAKAYLFVPV--TDNTSMEPIKGINWELVEKITKTTAWDNPLQRARPDV 1149
Query: 942 --------------------LKKN-----------------SSLDTESNCGALPSRN--- 1001
L+ N +S D G LP R+
Sbjct: 1150 YLGTNERTLGGDRREYGFGKLRHNIVFGQKSHPTYGIRGAVASFDVVRASGLLPVRDAFE 1209
Query: 1002 NSAETGSKAASLIHFANCVLDVHSLKDLVVLAIHTGKMYSIVEVVSNTSAESTFDGNSD- 1061
E L+ C++ L +V A H+GK + + + + SAE++F
Sbjct: 1210 KEVEEDLSKGKLMMADGCMV-AEDLIGKIVTAAHSGKRFYVDSICYDMSAETSFPRKEGY 1269
Query: 1062 RAQSDYINFADYFKKKYGITLRYPQQPMLRLKQSHNPHNLL-VNFNDEGETSDKLQSGHA 1121
+Y +ADY+K+KYG+ L QQP+++ + NLL F GE+ L +
Sbjct: 1270 LGPLEYNTYADYYKQKYGVDLNCKQQPLIKGRGVSYCKNLLSPRFEQSGESETVLDKTY- 1329
Query: 1122 SKKPQMHVHMPPELVVSMDVPKGVWKSTYLLPSLMHRLESLMLASQLRKEINYHSNSLCI 1181
+V +PPEL V + + + LPS+M R+ES++LA QL+ I+Y I
Sbjct: 1330 ------YVFLPPELCVVHPLSGSLIRGAQRLPSIMRRVESMLLAVQLKNLISYP-----I 1389
Query: 1182 SSSLIVEALTTLGCCESFSLERLELLGDSVLKYAVSCHLFLKFPEKHEGQLSSQRQQVIC 1241
+S I+EALT C E+F ER ELLGD+ LK+ VS LFLK+P+KHEGQL+ RQQ++
Sbjct: 1390 PTSKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVS 1449
Query: 1242 NATLHRLGIKCKLQGYIRDSAFDPRRWVAPGQRTTRPVPCKCG----VDTVEVPLDDKFC 1301
N L++ + LQ YI+ F P RW APG K G D + P+ ++
Sbjct: 1450 NMVLYQFALVKGLQSYIQADRFAPSRWSAPGVPPVFDEDTKDGGSSFFDEEQKPVSEENS 1509
Query: 1302 T--EDPKVVLGKCCD--KGHRWVVSKTIADCVEALIGAYYVNAGIDAALHVIKWLGIDAD 1361
ED ++ G+ +R + SKT+AD VEALIG YYV G AA H++KW+GI +
Sbjct: 1510 DVFEDGEMEDGELEGDLSSYRVLSSKTLADVVEALIGVYYVEGGKIAANHLMKWIGIHVE 1569
Query: 1362 LGVSLVVDAITSASLRSCILKETEIETLESKIGYEFAVKGLLLEAITHTSDQELGVNYCY 1421
V + + ++ +LK + LE + YEF KGLL+EAITH S GV+ CY
Sbjct: 1570 DDPDEVDGTLKNVNVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVS-CY 1629
Query: 1422 QRLEFLGDSVLDLLITWHYYQTHSDIDPGELTDLRSASVNNENFAQVAVRRNLQQHLQHC 1481
QRLEF+GD+VLD LIT H + T++ + PG LTDLR+A+VNNENFA+VAV+ L +L+H
Sbjct: 1630 QRLEFVGDAVLDHLITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHG 1689
Query: 1482 CGLLIGQITEYVKYL-SESLDAGKSLHG---NKGPKVLGDMVESIAGAILIDTELNLDEV 1541
L QI E+VK + +ES G + G K PKVLGD+VESIAGAI +D+ +
Sbjct: 1690 SSALEKQIREFVKEVQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAA 1749
Query: 1542 WRIYKPLLTPFVTPDKLELQPLRELIELCDSLGYFIKDKCTRKGEIIHAELRLQLQDVLL 1601
W++++PLL P VTP+ L + P+REL E C ++ K +R G E+ + D +
Sbjct: 1750 WKVFQPLLQPMVTPETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFI---DGVQ 1809
Query: 1602 IGEGYERTRKAARGEAAHRLLVQLETRGIYYSRSGSKKRKQNPNHVGECHLPGSWLRLIA 1625
+G +K A+ AA L L+ + I + SK++ N + GE
Sbjct: 1810 VGVAQNPQKKMAQKLAARNALAALKEKEI----AESKEKHINNGNAGEDQGENE------ 1869
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q5N870 | 0.0e+00 | 51.46 | Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica OX=39947 GN=DC... | [more] |
Q9LXW7 | 0.0e+00 | 51.01 | Endoribonuclease Dicer homolog 3 OS=Arabidopsis thaliana OX=3702 GN=DCL3 PE=1 SV... | [more] |
Q7XD96 | 0.0e+00 | 48.82 | Endoribonuclease Dicer homolog 3b OS=Oryza sativa subsp. japonica OX=39947 GN=DC... | [more] |
Q9SP32 | 2.1e-225 | 33.47 | Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV... | [more] |
Q8LMR2 | 7.3e-210 | 31.91 | Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... | [more] |
Match Name | E-value | Identity | Description | |
XP_022132010.1 | 0.0 | 97.23 | endoribonuclease Dicer homolog 3a isoform X1 [Momordica charantia] | [more] |
XP_022132011.1 | 0.0 | 96.97 | endoribonuclease Dicer homolog 3a isoform X2 [Momordica charantia] | [more] |
XP_038885573.1 | 0.0 | 86.06 | endoribonuclease Dicer homolog 3 isoform X1 [Benincasa hispida] | [more] |
XP_022132012.1 | 0.0 | 96.68 | endoribonuclease Dicer homolog 3a isoform X3 [Momordica charantia] | [more] |
XP_023547257.1 | 0.0 | 86.17 | endoribonuclease Dicer homolog 3a isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BR97 | 0.0 | 97.23 | endoribonuclease Dicer homolog 3a isoform X1 OS=Momordica charantia OX=3673 GN=L... | [more] |
A0A6J1BR19 | 0.0 | 96.97 | endoribonuclease Dicer homolog 3a isoform X2 OS=Momordica charantia OX=3673 GN=L... | [more] |
A0A6J1BSN5 | 0.0 | 96.68 | endoribonuclease Dicer homolog 3a isoform X3 OS=Momordica charantia OX=3673 GN=L... | [more] |
A0A1S3BDT0 | 0.0 | 85.31 | endoribonuclease Dicer homolog 3 OS=Cucumis melo OX=3656 GN=LOC103488578 PE=3 SV... | [more] |
A0A6J1KK54 | 0.0 | 85.44 | endoribonuclease Dicer homolog 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... | [more] |