MC08g0462 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g0462 vs. ExPASy Swiss-Prot
Match: Q9C5V5 (Glutamate receptor 2.8 OS=Arabidopsis thaliana OX=3702 GN=GLR2.8 PE=2 SV=2)

HSP 1 Score: 825.1 bits (2130), Expect = 8.5e-238
Identity = 451/942 (47.88%), Postives = 607/942 (64.44%), Query Frame = 0

Query: 39  MKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCI 98
           M P +     L +F  C+  LL         QN   ++ + VGVVLD  +   K+ L+ I
Sbjct: 1   MNPKKNNNTFLSYFV-CLFLLLE----VGLGQN--QISEIKVGVVLDLNTTFSKICLTSI 60

Query: 99  DMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANF 158
           +++LS+FY  +P+Y+TR+ LH R+    D V A+AAA+DLI+N++V AI+GP  SMQA F
Sbjct: 61  NLALSDFYKDHPNYRTRLALHVRD-SMKDTVQASAAALDLIQNEQVSAIIGPIDSMQAKF 120

Query: 159 VIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIY 218
           +I+L  K  VP +SF+ATSP L S++S YF R   +DS QV AI+AI ++FGWR VV IY
Sbjct: 121 MIKLANKTQVPTISFSATSPLLTSIKSDYFVRGTIDDSYQVKAIAAIFESFGWRSVVAIY 180

Query: 219 EDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLP 278
            D+E G+GI+PYL DALQDV      RSVI   A  DQI +ELYKL T Q+RVFVVHM  
Sbjct: 181 VDNELGEGIMPYLFDALQDVQVD---RSVIPSEANDDQILKELYKLMTRQTRVFVVHMAS 240

Query: 279 SLAARLFAIADKIGMMSEGFVWILTGATANLLQSM-DSSVLHSMDGALGVKTFVPKSMKL 338
            LA+R+F  A +IGMM EG+VW++T    ++++ +     L+++DG LGV++ VPKS  L
Sbjct: 241 RLASRIFEKATEIGMMEEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKSKGL 300

Query: 339 DEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYL----KNPN 398
           ++F +RWKR F  +NP L D  L +FGLWA+D+  ALAMAVE+   ++F Y      + N
Sbjct: 301 EDFRLRWKRNFKKENPWLRD-DLSIFGLWAYDSTTALAMAVEKTNISSFPYNNASGSSNN 360

Query: 399 LTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFW 458
           +TDL +L VS  G  L + LS  +F GLAG F + + +L+S   E++N  GN    VGFW
Sbjct: 361 MTDLGTLHVSRYGPSLLEALSEIRFNGLAGRFNLIDRQLESPKFEIINFVGNEERIVGFW 420

Query: 459 TPENGL-----TKDWSASGTR--SIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEF 518
           TP NGL      K  S +G R   +IWPG +   P+GWE+PT G ++K+GVP K G+  F
Sbjct: 421 TPSNGLVNVNSNKTTSFTGERFGPLIWPGKSTIVPKGWEIPTNGKKIKVGVPVKKGFFNF 480

Query: 519 VRI----TKNGTDAEGYCTDVFEAALAGLPYAVPFDYFPF---DGAYDELIMKVHDGFFD 578
           V +      N T  +GY  D+FEAAL  LPY+V   Y+ F   D  YD+L+ KV +G  D
Sbjct: 481 VEVITDPITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFESPDDDYDDLVYKVDNGTLD 540

Query: 579 GAVGDITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFF 638
             VGD+TI A RS Y DFTLP+TESGVSM+VP + N     WVFLKP  LDLW+T+ CFF
Sbjct: 541 AVVGDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVTTACFF 600

Query: 639 VFMGFVVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFV 698
           V +GFVVW+ EHR+N DFRGPP HQIGTS WFSF TMVFA RE ++SNLARFVVV+W FV
Sbjct: 601 VLIGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVVVWCFV 660

Query: 699 VFILTQSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGF--KNL 758
           V +LTQSYTA+LTS LTVQ+ QP+  ++ +L+K   +VGYQ G+FV   L   GF    L
Sbjct: 661 VLVLTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKEGFNVSKL 720

Query: 759 RPYASPDELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFP 818
           +P+ S +E H       SNG   AAFDE+ Y++  LS++  KY + +P +K  GFGFAFP
Sbjct: 721 KPFGSSEECHALL----SNGSISAAFDEVAYLRAILSQYCSKYAIVEPTFKTAGFGFAFP 780

Query: 819 MGSPLVADISRQVLNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIA 878
             SPL  D+S+ +LNVT+ ++M  I+ KW  +  + P     + S+RL+L SFWGLFLIA
Sbjct: 781 RNSPLTGDVSKAILNVTQGDEMQHIENKWFMKQNDCPDPKTALSSNRLSLRSFWGLFLIA 840

Query: 879 GSAAVVALLIYFAIFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKS 938
           G A+ +ALLI+  +FLY+  HTL     D+  +S + RKL +L R +D +D+ SHTF+ S
Sbjct: 841 GIASFLALLIFVFLFLYENRHTL----CDDSEDS-IWRKLTSLFRNFDEKDIKSHTFKSS 900

Query: 939 NNINVEAHAMDGASPSSNCPPSPSNYSV--------QDASFE 952
              +V       +SP +   PSPS   +        QD +FE
Sbjct: 901 AVHHV-------SSPMTQYIPSPSTLQIAPRPHSPSQDRAFE 914

BLAST of MC08g0462 vs. ExPASy Swiss-Prot
Match: Q8LGN0 (Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3)

HSP 1 Score: 811.2 bits (2094), Expect = 1.3e-233
Identity = 444/915 (48.52%), Postives = 596/915 (65.14%), Query Frame = 0

Query: 56  VVFLLGTTALTAA-AQNATAMAAVNVGVVLDFESRIGKMGLSCIDMSLSEFYAANPDYKT 115
           V+F+ G   +     QN T    + VGVVLD  +   K+ L+ I++SLS+FY  + DY T
Sbjct: 18  VLFVCGFVLMEGCLGQNQT--TEIKVGVVLDLHTSFSKLCLTSINISLSDFYKYHSDYTT 77

Query: 116 RIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANFVIELGQKAHVPILSFT 175
           R+ +H R+   +DVV A++AA+DLIKN++V AI+GP +SMQA F+I L  K+ VP ++F+
Sbjct: 78  RLAIHIRD-SMEDVVQASSAALDLIKNEQVSAIIGPRTSMQAEFMIRLADKSQVPTITFS 137

Query: 176 ATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIYEDDEFGDGILPYLIDA 235
           AT P L S+ SPYF R   +DS QV AI+AIVK+FGWR VV IY D+EFG+GILP L DA
Sbjct: 138 ATCPLLTSINSPYFVRATLDDSSQVKAIAAIVKSFGWRNVVAIYVDNEFGEGILPLLTDA 197

Query: 236 LQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLPSLAARLFAIADKIGMM 295
           LQDV A V  R +I   A  DQI +ELYKL TMQ+RVFVVHM P+L  R F  A +IGMM
Sbjct: 198 LQDVQAFVVNRCLIPQEANDDQILKELYKLMTMQTRVFVVHMPPTLGFRFFQKAREIGMM 257

Query: 296 SEGFVWILTGATANLLQSMD-SSVLHSMDGALGVKTFVPKSMKLDEFTVRWKRKFLSQNP 355
            EG+VW+LT    NLL+S +  S L +M G LGV++ +PKS KL  F +RW++ F  +  
Sbjct: 258 EEGYVWLLTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSKKLKNFRLRWEKMFPKKG- 317

Query: 356 TLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTY----LKNPNLTDLQSLGVSENGEKL 415
             ND ++++F L A+D+  ALAMAVE+    +  Y        N T+L +LGVS  G  L
Sbjct: 318 --NDEEMNIFALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSL 377

Query: 416 RDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTPENGLTKDWSASGTR 475
              LS  +F GLAG+F + NG+L+S+  +V+N+ G+    +G W P NG+    S + T 
Sbjct: 378 LKALSNVRFNGLAGEFELINGQLESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTS 437

Query: 476 -------SIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRI----TKNGTDAEG 535
                   +IWPG +   P+GW++PT G  L++G+P K G+ EFV        N     G
Sbjct: 438 VLGERLGPVIWPGKSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTG 497

Query: 536 YCTDVFEAALAGLPYAVPFDYFPF---DGAYDELIMKVHDGFFDGAVGDITIVANRSQYV 595
           YC ++FEA L  LPY+V   Y  F   D  YDE++ +V+ G +D  VGD+TIVANRS YV
Sbjct: 498 YCIEIFEAVLKKLPYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYV 557

Query: 596 DFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILEHRINE 655
           DFTLP+TESGVSM+VP + N     WVFL+P +LDLW+T+ CFFVF+GF+VWILEHR+N 
Sbjct: 558 DFTLPYTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNT 617

Query: 656 DFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTASLTSLL 715
           DFRGPP HQIGTS WF+F TM FA RE ++SNLARFVV++W FVV +L QSYTA+LTS  
Sbjct: 618 DFRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFF 677

Query: 716 TVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGF--KNLRPYASPDELHKFFKRG 775
           TV+ LQP++T+  +L+K    +GYQ G+FV  LL+S GF    L+P+ S  E  + F   
Sbjct: 678 TVKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQGFDESQLKPFGSAVECDELF--- 737

Query: 776 SSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQVLNV 835
            SNG   A+FDE+ YIK+ LS+   KYTM +P +K  GFGF FP  SPL  D+SR +LNV
Sbjct: 738 -SNGTITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNV 797

Query: 836 TESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFAIFL 895
           T+ E+M  I+ KW  +  N P    ++ S+ L+LSSFWGLFLIAG A+ +ALLI+ A FL
Sbjct: 798 TQGEEMQHIENKWFKKPNNCPDLNTSLSSNHLSLSSFWGLFLIAGIASFLALLIFVANFL 857

Query: 896 YKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGAS-- 944
           Y+  HTL     D+  NS    KL+ L+R +D +D+ SH F+++   NV +    G+S  
Sbjct: 858 YEHKHTL----FDDSENS-FRGKLKFLVRNFDEKDIKSHMFKENAVHNVSSPITQGSSSP 915

BLAST of MC08g0462 vs. ExPASy Swiss-Prot
Match: O81078 (Glutamate receptor 2.9 OS=Arabidopsis thaliana OX=3702 GN=GLR2.9 PE=2 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 1.4e-232
Identity = 440/944 (46.61%), Postives = 614/944 (65.04%), Query Frame = 0

Query: 55  CVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCIDMSLSEFYAANPDYKT 114
           C   L+G        QN T  + + VGVVLD  +   K+ L+ I M++S+FYA +P+Y T
Sbjct: 13  CGFLLMG----VGLGQNQT--SEIKVGVVLDLNTTFSKICLTSIKMAVSDFYADHPNYLT 72

Query: 115 RIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANFVIELGQKAHVPILSFT 174
           R+ LH R+   +D V A+AAA+DLIK ++V AI+GP +SMQA+F+I+L  K  VP ++F+
Sbjct: 73  RLTLHVRD-SMEDTVQASAAALDLIKTEQVSAIIGPINSMQADFMIKLANKTQVPTITFS 132

Query: 175 ATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIYEDDEFGDGILPYLIDA 234
           ATSP L S++SPYF R   +DS QV AI++I K F WR+VV IY D+EFG+G +P+L DA
Sbjct: 133 ATSPLLTSIKSPYFVRATIDDSSQVRAIASIFKFFRWRRVVAIYVDNEFGEGFMPFLFDA 192

Query: 235 LQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLPSLAARLFAIADKIGMM 294
           LQDV  +   RSVI P A  D+I +EL KL   Q+RVFVVHM  SLA R+F IA  IGMM
Sbjct: 193 LQDVEVK---RSVIPPEAIDDEIQKELRKLMERQARVFVVHMESSLALRVFQIARDIGMM 252

Query: 295 SEGFVWILTGATANLLQSMDSS-VLHSMDGALGVKTFVPKSMKLDEFTVRWKRKFLSQNP 354
            EG+VW++T    ++++ +++   L++++G LGV++ VPKS +L +F +RWKR F  +NP
Sbjct: 253 EEGYVWLMTNGMTHMMRHINNGRSLNTIEGVLGVRSHVPKSKELGDFRLRWKRTFEKENP 312

Query: 355 TLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTY----LKNPNLTDLQSLGVSENGEKL 414
           ++ D  L+VF LWA+D+  ALA AVE+    +  Y      + N TDL ++GVS  G  L
Sbjct: 313 SMRD-DLNVFALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSL 372

Query: 415 RDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTPENGLTKDWSASGTR 474
           +   S  +F GLAG+F + +G+LQS   E++N  GN    +GFWTP +GL  D ++S  +
Sbjct: 373 QKAFSEVRFNGLAGEFKLIDGQLQSPKFEIINFVGNEERIIGFWTPRDGL-MDATSSNKK 432

Query: 475 S---IIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRIT----KNGTDAEGYCTD 534
           +   +IWPG +   P+GWE+P  G +L++GVP K G+ +FV++T     N     GY  +
Sbjct: 433 TLGPVIWPGKSKIVPKGWEIP--GKKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIE 492

Query: 535 VFEAALAGLPYAVPFDYFPFDGA--YDELIMKVHDGFFDGAVGDITIVANRSQYVDFTLP 594
           +FEAAL  LPY V  +Y  F+    Y+ L+ +V+D  +D  VGDITI ANRS Y DFTLP
Sbjct: 493 IFEAALKELPYLVIPEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLP 552

Query: 595 FTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILEHRINEDFRGP 654
           FTESGVSM+VP + N     WVFL+P +L+LW+T+ CFFVF+GFVVW+ EHR+N DFRGP
Sbjct: 553 FTESGVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGP 612

Query: 655 PSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTASLTSLLTVQQL 714
           P +QIGTSLWFSF TMVFA RE ++SNLARFVVV+W FVV +LTQSYTASLTS LTVQ L
Sbjct: 613 PQYQIGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSL 672

Query: 715 QPSITDINELMKRQPWVGYQEGSFVLGLLRSVGF--KNLRPYASPDELHKFFKRGSSNGG 774
           QP++T++N+L+K +  VGYQ G+FV  +L  +GF    L+P+ S  +      +G S  G
Sbjct: 673 QPTVTNVNDLIKNRDCVGYQGGAFVKDILLGLGFHEDQLKPFDSAKDADDLLSKGKSK-G 732

Query: 775 FDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQVLNVTESEK 834
             AAFDE+ Y+K  LS+   KY M +P +K  GFGFAFP  SPL  + SR +LN+T++  
Sbjct: 733 IAAAFDEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNV 792

Query: 835 MNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFAIFLYKEHH 894
             QI+ +W  +  + P     + S+RLNLSSF GLFLIAG+A   +LL++ A+FLY+  H
Sbjct: 793 TQQIEDRWFPKKNDCPDPMTALSSNRLNLSSFLGLFLIAGTAISFSLLVFVALFLYEHRH 852

Query: 895 TLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGASPSSNCPP 954
           TL      + S   + RKL+ L + +D +D+ SHTF+ S   N+       +SP ++  P
Sbjct: 853 TL-----GDDSEDSLWRKLKFLFKIFDEKDMNSHTFKNSAIHNI-------SSPMTHKTP 912

Query: 955 SPSNYSV--------QDASFEFFNGSGDSSPMNRNQTPPLHNQE 975
           SPS   +        Q+  FE    S   S       P +H+++
Sbjct: 913 SPSTVQITPWPQSPSQNREFELRRVSFSPSEERFTTQPIIHHED 929

BLAST of MC08g0462 vs. ExPASy Swiss-Prot
Match: O04660 (Glutamate receptor 2.1 OS=Arabidopsis thaliana OX=3702 GN=GLR2.1 PE=2 SV=2)

HSP 1 Score: 772.3 bits (1993), Expect = 6.6e-222
Identity = 416/883 (47.11%), Postives = 570/883 (64.55%), Query Frame = 0

Query: 39  MKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCI 98
           MK     V  L FF   +VFL+        AQN   +  VNVG+V D  +    M L CI
Sbjct: 1   MKRENNLVLSLLFFV--IVFLM----QVGEAQN--RITNVNVGIVNDIGTAYSNMTLLCI 60

Query: 99  DMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANF 158
           +MSLS+FY+++P+ +TR+V    +  N DVV AAAAA+DLI NK+V+AILGP +SMQA F
Sbjct: 61  NMSLSDFYSSHPETQTRLVTTVVDSKN-DVVTAAAAALDLITNKEVKAILGPWTSMQAQF 120

Query: 159 VIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIY 218
           +IE+GQK+ VPI++++ATSP+LAS+RS YFFR   +DS QV AI  I+K FGWR+V P+Y
Sbjct: 121 MIEMGQKSQVPIVTYSATSPSLASIRSQYFFRATYDDSSQVHAIKEIIKLFGWREVAPVY 180

Query: 219 EDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLP 278
            DD FG+GI+P L D LQ++N R+ YR+VI+P AT D+I  EL ++ T+ +RVFVVH++ 
Sbjct: 181 VDDTFGEGIMPRLTDVLQEINVRIPYRTVISPNATDDEISVELLRMMTLPTRVFVVHLVE 240

Query: 279 SLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKLD 338
            LA+R FA A +IG+M +G+VWILT    ++L  M+ + + +M G LGVKT+VP+S +L+
Sbjct: 241 LLASRFFAKATEIGLMKQGYVWILTNTITDVLSIMNETEIETMQGVLGVKTYVPRSKELE 300

Query: 339 EFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK---NPNLT 398
            F  RW ++F      ++D  L+V+GLWA+DA  ALA+A+E  G +N T++K     N++
Sbjct: 301 NFRSRWTKRF-----PISD--LNVYGLWAYDATTALALAIEEAGTSNLTFVKMDAKRNVS 360

Query: 399 DLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTP 458
           +LQ LGVS+ G KL   LS  +F+GLAGDF   NGELQ +  E+VNVNG  G  +GFW  
Sbjct: 361 ELQGLGVSQYGPKLLQTLSRVRFQGLAGDFQFINGELQPSVFEIVNVNGQGGRTIGFWMK 420

Query: 459 ENGLTKDWS---ASGT---------RSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGY 518
           E GL K+     AS T         R IIWPGDT + P+GWE+PT G RL+IGVP  + +
Sbjct: 421 EYGLFKNVDQKPASKTTFSSWQDRLRPIIWPGDTTSVPKGWEIPTNGKRLQIGVPVNNTF 480

Query: 519 SEFVRITK----NGTDAEGYCTDVFEAALAGLPYAVPFDYFPF-DGAYDELIMKVHDGFF 578
            +FV+ T+    N T   G+  D FEA +  +PY + +D+ PF DG YD L+ +V+ G +
Sbjct: 481 QQFVKATRDPITNSTIFSGFSIDYFEAVIQAIPYDISYDFIPFQDGGYDALVYQVYLGKY 540

Query: 579 DGAVGDITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCF 638
           D  V D TI +NRS YVDF+LP+T SGV +VVP + + +  + +FL PLTL LW+ S   
Sbjct: 541 DAVVADTTISSNRSMYVDFSLPYTPSGVGLVVPVKDSVRRSSTIFLMPLTLALWLISLLS 600

Query: 639 FVFMGFVVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFF 698
           F  +G VVW+LEHR+N DF GP  +Q+ T  WFSF  MVFA RE ++S  AR VV+IW+F
Sbjct: 601 FFIIGLVVWVLEHRVNPDFDGPGQYQLSTIFWFSFSIMVFAPRERVLSFWARVVVIIWYF 660

Query: 699 VVFILTQSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFK--N 758
           +V +LTQSYTASL SLLT Q L P++T+IN L+ +   VGYQ  SF+LG LR  GF   +
Sbjct: 661 LVLVLTQSYTASLASLLTTQHLHPTVTNINSLLAKGESVGYQ-SSFILGRLRDSGFSEAS 720

Query: 759 LRPYASPDELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAF 818
           L  Y SP+       +G + GG  A   E+PY+++FL ++ +KY M    +K DG GF F
Sbjct: 721 LVSYGSPEHCDALLSKGQAEGGVSAVLMEVPYVRIFLGQYCNKYKMVQTPFKVDGLGFVF 780

Query: 819 PMGSPLVADISRQVLNVTESEKMNQIQKKW---LGENC----NSPSSGGNVGSSRLNLSS 878
           P+GSPLVADISR +L V ES K NQ++  W   + E+C     +P    +V   +L   S
Sbjct: 781 PIGSPLVADISRAILKVEESNKANQLENAWFKPIDESCPDPLTNPDPNPSVSFRQLGFDS 840

Query: 879 FWGLFLIAGSAAVVALLIYFAIFLYKEHHTLRRTAADEGSNSP 893
           FW LFL+A     +ALL +   FL +  +        E  N P
Sbjct: 841 FWVLFLVAAIVCTMALLKFVYQFLKENPNQRNLRVLWEKFNEP 866

BLAST of MC08g0462 vs. ExPASy Swiss-Prot
Match: Q9SHV1 (Glutamate receptor 2.2 OS=Arabidopsis thaliana OX=3702 GN=GLR2.2 PE=2 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.8e-216
Identity = 396/856 (46.26%), Postives = 566/856 (66.12%), Query Frame = 0

Query: 57  VFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCIDMSLSEFYAANPDYKTRI 116
           +FL     L ++         VN+GVV D  +    + + CI+MSL++FY++ P ++TR+
Sbjct: 11  LFLFFFFCLESSRGQDNGKTQVNIGVVSDVGTSYPDVAMLCINMSLADFYSSRPQFQTRL 70

Query: 117 VLHTRNVGN--DDVVGAAAAAVDLIKNKKVQAILGPTSSMQANFVIELGQKAHVPILSFT 176
           V+   NVG+  +DVVGAA AA+DLIKNK+V+AILGP +SMQA+F+IE+GQK+ VP++S++
Sbjct: 71  VV---NVGDSKNDVVGAATAAIDLIKNKQVKAILGPWTSMQAHFLIEIGQKSRVPVVSYS 130

Query: 177 ATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIYEDDEFGDGILPYLIDA 236
           ATSP+L SLRSPYFFR    DS QV AI AI+K FGWR+VVP+Y D+ FG+GI+P L D+
Sbjct: 131 ATSPSLTSLRSPYFFRATYEDSSQVHAIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDS 190

Query: 237 LQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLPSLAARLFAIADKIGMM 296
           LQD+N R+ YRSVI   AT   I  EL K+  M +RVF+VHM  SLA+ +F  A ++G+M
Sbjct: 191 LQDINVRIPYRSVIPLNATDQDISVELLKMMNMPTRVFIVHMSSSLASTVFIKAKELGLM 250

Query: 297 SEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKLDEFTVRWKRKFLSQNPT 356
             G+VWILT    + L+S++ + + +M+G LG+KT++PKS  L+ F  RWKR+F    P 
Sbjct: 251 KPGYVWILTNGVMDGLRSINETGIEAMEGVLGIKTYIPKSKDLETFRSRWKRRF----PQ 310

Query: 357 LNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK---NPNLTDLQSLGVSENGEKLRD 416
           +   +L+V+GLWA+DA  ALAMA+E  G  N T+       N+++L  LG+S+ G KL  
Sbjct: 311 M---ELNVYGLWAYDATTALAMAIEDAGINNMTFSNVDTGKNVSELDGLGLSQFGPKLLQ 370

Query: 417 FLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTPENGLTK----DWSASG 476
            +ST +F+GLAGDF   +G+LQ +  E+VN+ G     +GFWT  NGL K    +  + G
Sbjct: 371 TVSTVQFKGLAGDFHFVSGQLQPSVFEIVNMIGTGERSIGFWTEGNGLVKKLDQEPRSIG 430

Query: 477 TRS--------IIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITK----NGTD 536
           T S        IIWPG+  + P+GWE+PT G +L+IGVP++ G+++ V++T+    N T 
Sbjct: 431 TLSTWPDHLKHIIWPGEAVSVPKGWEIPTNGKKLRIGVPKRIGFTDLVKVTRDPITNSTV 490

Query: 537 AEGYCTDVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGDITIV 596
            +G+C D FEA +  +PY V +++FPF+       G +++L+ +V+ G FD  VGD TI+
Sbjct: 491 VKGFCIDFFEAVIQAMPYDVSYEFFPFEKPNGEPAGNHNDLVHQVYLGQFDAVVGDTTIL 550

Query: 597 ANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWI 656
           ANRS +VDFTLPF +SGV ++VP +   K   + FLKPL+++LW+T+  FF  +G  VW 
Sbjct: 551 ANRSSFVDFTLPFMKSGVGLIVPLKDEVKRDKFSFLKPLSIELWLTTLVFFFLVGISVWT 610

Query: 657 LEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYT 716
           LEHR+N DFRGP ++Q  T  WF+F TMVFA RE ++S  AR +VV W+FV+ +LTQSYT
Sbjct: 611 LEHRVNSDFRGPANYQASTIFWFAFSTMVFAPRERVLSFGARSLVVTWYFVLLVLTQSYT 670

Query: 717 ASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGF--KNLRPYASPDEL 776
           ASL SLLT QQL P+IT ++ L+ R   VGYQ  SF+LG L   GF   +L P+ + +E 
Sbjct: 671 ASLASLLTSQQLNPTITSMSSLLHRGETVGYQRTSFILGKLNETGFPQSSLVPFDTAEEC 730

Query: 777 HKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADI 836
            +  K+G  NGG  AAF   PY++LFL ++ + Y M +  +  DGFGF FP+GSPLVAD+
Sbjct: 731 DELLKKGPKNGGVAAAFLGTPYVRLFLGQYCNTYKMVEEPFNVDGFGFVFPIGSPLVADV 790

Query: 837 SRQVLNVTESEKMNQIQKKWL---GENC----NSPSSGGNVGSSRLNLSSFWGLFLIAGS 876
           SR +L V ES K  +++  W     ++C     +P S   V + +L + SFW LFL+   
Sbjct: 791 SRAILKVAESPKAVELEHAWFKKKEQSCPDPVTNPDSNPTVTAIQLGVGSFWFLFLVVFV 850

BLAST of MC08g0462 vs. NCBI nr
Match: XP_022131338.1 (glutamate receptor 2.8-like [Momordica charantia])

HSP 1 Score: 1914 bits (4957), Expect = 0.0
Identity = 961/962 (99.90%), Postives = 962/962 (100.00%), Query Frame = 0

Query: 31  MHRQDPPKMKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRI 90
           MHRQDPPKMKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVV+DFESRI
Sbjct: 1   MHRQDPPKMKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVMDFESRI 60

Query: 91  GKMGLSCIDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGP 150
           GKMGLSCIDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGP
Sbjct: 61  GKMGLSCIDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGP 120

Query: 151 TSSMQANFVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFG 210
           TSSMQANFVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFG
Sbjct: 121 TSSMQANFVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFG 180

Query: 211 WRQVVPIYEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSR 270
           WRQVVPIYEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSR
Sbjct: 181 WRQVVPIYEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSR 240

Query: 271 VFVVHMLPSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTF 330
           VFVVHMLPSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTF
Sbjct: 241 VFVVHMLPSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTF 300

Query: 331 VPKSMKLDEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK 390
           VPKSMKLDEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK
Sbjct: 301 VPKSMKLDEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK 360

Query: 391 NPNLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRV 450
           NPNLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRV
Sbjct: 361 NPNLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRV 420

Query: 451 GFWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRIT 510
           GFWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRIT
Sbjct: 421 GFWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRIT 480

Query: 511 KNGTDAEGYCTDVFEAALAGLPYAVPFDYFPFDGAYDELIMKVHDGFFDGAVGDITIVAN 570
           KNGTDAEGYCTDVFEAALAGLPYAVPFDYFPFDGAYDELIMKVHDGFFDGAVGDITIVAN
Sbjct: 481 KNGTDAEGYCTDVFEAALAGLPYAVPFDYFPFDGAYDELIMKVHDGFFDGAVGDITIVAN 540

Query: 571 RSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILE 630
           RSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILE
Sbjct: 541 RSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILE 600

Query: 631 HRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTAS 690
           HRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTAS
Sbjct: 601 HRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTAS 660

Query: 691 LTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELHKFF 750
           LTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELHKFF
Sbjct: 661 LTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELHKFF 720

Query: 751 KRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQV 810
           KRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQV
Sbjct: 721 KRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQV 780

Query: 811 LNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFA 870
           LNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFA
Sbjct: 781 LNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFA 840

Query: 871 IFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGA 930
           IFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGA
Sbjct: 841 IFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGA 900

Query: 931 SPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLELTSGEEITEIIV 990
           SPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLELTSGEEITEIIV
Sbjct: 901 SPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLELTSGEEITEIIV 960

Query: 991 NN 992
           NN
Sbjct: 961 NN 962

BLAST of MC08g0462 vs. NCBI nr
Match: XP_038884294.1 (glutamate receptor 2.8-like [Benincasa hispida])

HSP 1 Score: 1367 bits (3539), Expect = 0.0
Identity = 700/959 (72.99%), Postives = 793/959 (82.69%), Query Frame = 0

Query: 49  LCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCIDMSLSEFYAA 108
           LCF AGC VF   TTA     QNA+ +  V+VGVVLD ES IGKMGLSCIDMSLS+FY+ 
Sbjct: 9   LCFLAGCAVFFATTTA-----QNASTVP-VDVGVVLDMESWIGKMGLSCIDMSLSDFYSL 68

Query: 109 NPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANFVIELGQKAHV 168
           NP YKTRIVLH R+ G D VVGAAAAAVDLIKN KV AILGPT+SMQANFVIELGQKAHV
Sbjct: 69  NPHYKTRIVLHPRDSGRD-VVGAAAAAVDLIKNNKVHAILGPTTSMQANFVIELGQKAHV 128

Query: 169 PILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIYEDDEFGDGIL 228
           PIL+FTA+SPALASLRSPYFFRL QNDS QV AISA+VK++ WRQVVPIYEDDEFGDG+L
Sbjct: 129 PILTFTASSPALASLRSPYFFRLTQNDSAQVTAISALVKSYNWRQVVPIYEDDEFGDGML 188

Query: 229 PYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLPSLAARLFAIA 288
           PYLID+LQ VNARV YRSVI+P+AT DQI EELYKLTTMQ+RVFVVHM+PSLAARLF  A
Sbjct: 189 PYLIDSLQSVNARVPYRSVIDPSATDDQIEEELYKLTTMQTRVFVVHMVPSLAARLFMKA 248

Query: 289 DKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKLDEFTVRWKRKF 348
           ++IGMM+EG+ WILTG T N+L SMDSSVL++M+GALGVK +VPKS++LD F +RWKRKF
Sbjct: 249 NEIGMMTEGYAWILTGVTTNVLDSMDSSVLNAMEGALGVKAYVPKSLELDSFKIRWKRKF 308

Query: 349 LSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLKNP------NLTDLQSLGV 408
           L +N  LN+PQLDVFGLWAHDAA+ALAMAVE+ G   F Y  NP      NLTDLQ+LGV
Sbjct: 309 LIENHILNEPQLDVFGLWAHDAARALAMAVEKTGDTEFRYKNNPINESKNNLTDLQTLGV 368

Query: 409 SENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTPENGLTKD 468
           SENGEK+R+ L   KF+ L GD+ +  GELQSA+ E+VNVNGN   RVGFW  ENGLTK+
Sbjct: 369 SENGEKIREALWEMKFKALTGDYRIVKGELQSASFEIVNVNGNGEKRVGFWNVENGLTKN 428

Query: 469 WSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITKNGTDAEGYCTD 528
            S SGT+ +IWPGDTAA+P+GWE P  G +L+IGVP K+GYSEFVR+ KNGT AEGYC D
Sbjct: 429 LSQSGTKPVIWPGDTAAEPKGWEWPVAGKKLRIGVPVKEGYSEFVRVWKNGTGAEGYCMD 488

Query: 529 VFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGDITIVANRSQYV 588
           VF+AA+  LPYAVPFDY PF        G+YD+LI+KV++GFFDGAVGDITI+ANRS YV
Sbjct: 489 VFDAAIGMLPYAVPFDYVPFAFPNGSSAGSYDDLIIKVNEGFFDGAVGDITIIANRSNYV 548

Query: 589 DFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILEHRINE 648
           DFTLPFTESGVSMVVPTQ NSKNRAW+FLKPLTLDLWITSFCFFVFMGFVVWILEHR NE
Sbjct: 549 DFTLPFTESGVSMVVPTQGNSKNRAWLFLKPLTLDLWITSFCFFVFMGFVVWILEHRTNE 608

Query: 649 DFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTASLTSLL 708
            FRGPPSHQIGTSLWFSFCTM FAQRE+L+SNLARFVV+IWFFVVFILTQSYTASLTSLL
Sbjct: 609 QFRGPPSHQIGTSLWFSFCTMAFAQRESLVSNLARFVVLIWFFVVFILTQSYTASLTSLL 668

Query: 709 TVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELHKFFKRGSS 768
           TVQQLQP+ITDIN L+K QPWVGYQ+GSFV GLLRSVG + LRPY +PD+LH+ F+ GS+
Sbjct: 669 TVQQLQPTITDINVLLKNQPWVGYQDGSFVFGLLRSVGIEKLRPYDAPDQLHEMFRLGSN 728

Query: 769 NGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQVLNVTE 828
           NGG DAAFDEI Y+KLFL KFPDKY M+DP YK DGFGFAFPMGSPLVAD+SR VLNVTE
Sbjct: 729 NGGIDAAFDEISYVKLFLFKFPDKYIMADPSYKTDGFGFAFPMGSPLVADVSRAVLNVTE 788

Query: 829 SEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFAIFLYK 888
           SEKMNQIQKKW GE CNS  +G  V SSRLNL SFWGLFLI GSAA++AL++YF IFL K
Sbjct: 789 SEKMNQIQKKWFGEQCNSLPTGSKVTSSRLNLDSFWGLFLITGSAAIIALIVYFIIFLCK 848

Query: 889 EHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSN--NINVEAHAMDGASPS 948
           E HTLRRT  +EGSNS    K+RALL+TYD RDLTSHTFRKSN    +     +DG S  
Sbjct: 849 EQHTLRRTV-NEGSNSSFRDKIRALLKTYDQRDLTSHTFRKSNLHQGDKTIRMIDGGSVK 908

Query: 949 SNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLELTSGEEITEIIVNN 992
           ++  PS SNYSV D +FE F+ SG+SSPMN      +    +   L +GEEITEI VNN
Sbjct: 909 AS--PSRSNYSVNDTNFELFSESGNSSPMNHRALEMVVRPTMETALGNGEEITEIHVNN 957

BLAST of MC08g0462 vs. NCBI nr
Match: XP_008464886.2 (PREDICTED: glutamate receptor 2.9-like isoform X1 [Cucumis melo])

HSP 1 Score: 1363 bits (3528), Expect = 0.0
Identity = 702/973 (72.15%), Postives = 794/973 (81.60%), Query Frame = 0

Query: 41  PNRGAVRV---LCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSC 100
           PN  A  +   L F AG   F       TAAAQN +A+  VNVGVVLD ES IGKMGLSC
Sbjct: 4   PNPAAAIIPAALYFLAGLFFFT------TAAAQNESAVP-VNVGVVLDMESWIGKMGLSC 63

Query: 101 IDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQAN 160
           IDMSLSEFY+ NP Y TRIVLH ++ G D VVGAAAAA+DLIKN KV AILGPT+SMQAN
Sbjct: 64  IDMSLSEFYSLNPHYHTRIVLHPKDSGRD-VVGAAAAALDLIKNNKVHAILGPTTSMQAN 123

Query: 161 FVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPI 220
           FVIELGQKAHVPIL+FTA+SPALASLRSPYFFRL QNDS QV AIS +VK++ WRQV+PI
Sbjct: 124 FVIELGQKAHVPILTFTASSPALASLRSPYFFRLTQNDSAQVVAISDLVKSYNWRQVIPI 183

Query: 221 YEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHML 280
           YEDDEFGDG+LPYLIDALQ VNARV YRSVI+P AT DQI EELYKL TMQ RVFVVHM+
Sbjct: 184 YEDDEFGDGMLPYLIDALQSVNARVPYRSVIDPEATDDQIKEELYKLMTMQPRVFVVHMV 243

Query: 281 PSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKL 340
           PSLAARLF +A++IGMMSEG+ WILT  T N+L SMDSSVL+SM+GALGVKT+VP S++L
Sbjct: 244 PSLAARLFMMANEIGMMSEGYAWILTDVTTNVLDSMDSSVLNSMEGALGVKTYVPNSLEL 303

Query: 341 DEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLKNP----- 400
           D F +RWKRKFL +NP   +PQLDVFGLWAHDAA+ALAMAVE+ G   F Y  NP     
Sbjct: 304 DGFKIRWKRKFLIENPIPKEPQLDVFGLWAHDAARALAMAVEKTGETEFKYKNNPINESN 363

Query: 401 -NLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVG 460
            NLTDLQ+LGVSENGEK+RD L  T+F+GL GD+ +  GELQS   E+VNVNGN G RVG
Sbjct: 364 NNLTDLQTLGVSENGEKIRDVLLKTRFKGLTGDYRIVKGELQSENFEIVNVNGNGGKRVG 423

Query: 461 FWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITK 520
           FW PE GLTK+ S SGT+ +IWPGDTA  PRGW  P  G RLKIG P K+GYSEFVR+ K
Sbjct: 424 FWNPEKGLTKNLSQSGTKPVIWPGDTATVPRGWVWPVAGKRLKIGFPAKEGYSEFVRVKK 483

Query: 521 NGTDAEGYCTDVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGD 580
           NGT AEGYCTDVF+A +A LPYAVP+DY PF        G+YD+LIM+V+ G +DGAVGD
Sbjct: 484 NGTGAEGYCTDVFDAVMAMLPYAVPYDYVPFAFPNGSSAGSYDDLIMQVYKGIYDGAVGD 543

Query: 581 ITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGF 640
           ITIVANRS YVDFTLPFTESGVSMVVPTQ  SKN+AW+FLKPLTLDLWITSFCFFVFMGF
Sbjct: 544 ITIVANRSNYVDFTLPFTESGVSMVVPTQGYSKNKAWIFLKPLTLDLWITSFCFFVFMGF 603

Query: 641 VVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILT 700
           VVWILEHRINE+FRGPPSHQIGTSLWFSFCTMVFAQRE+L+SNLARFVVVIWFFVVFILT
Sbjct: 604 VVWILEHRINEEFRGPPSHQIGTSLWFSFCTMVFAQRESLVSNLARFVVVIWFFVVFILT 663

Query: 701 QSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPD 760
           QSYTASLTSLLTVQQLQP+ITD+NEL+K QPWVGYQ+GSFV  LL+SVG KNLRPY +P+
Sbjct: 664 QSYTASLTSLLTVQQLQPTITDVNELLKNQPWVGYQDGSFVFELLKSVGIKNLRPYDTPE 723

Query: 761 ELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVA 820
           +L + FK GSSNGG DAAFDEIPY+KLFL KFPDKY M++P YK DGFGFAFP+GSPLV 
Sbjct: 724 QLDEMFKSGSSNGGIDAAFDEIPYVKLFLFKFPDKYIMAEPNYKTDGFGFAFPIGSPLVG 783

Query: 821 DISRQVLNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVA 880
           D+SR VLNVTESEKMN+IQK W G  CNS SSG  V SSRLNL SFWGLFLIAGSAA++A
Sbjct: 784 DVSRAVLNVTESEKMNRIQKTWFGGQCNSVSSGSKVTSSRLNLGSFWGLFLIAGSAAIIA 843

Query: 881 LLIYFAIFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEA 940
           LL+Y  IF +KE HTLR TA +EGSN+    K+RALL+TYD RDLTSHTF+KSN ++ + 
Sbjct: 844 LLVYGFIFFHKEQHTLRHTA-NEGSNNSFRHKIRALLKTYDERDLTSHTFKKSNLVHGDK 903

Query: 941 --HAMDG----ASPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLE 991
              AMDG    ASP SN PPSPSNYSV D SFEF++ SG++SPMN      + +  +   
Sbjct: 904 TIRAMDGSSISASPRSNYPPSPSNYSVHDTSFEFYSESGNASPMNHQALEMVVSTSMEAS 963

BLAST of MC08g0462 vs. NCBI nr
Match: KAA0038474.1 (glutamate receptor 2.9-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1358 bits (3516), Expect = 0.0
Identity = 701/973 (72.05%), Postives = 793/973 (81.50%), Query Frame = 0

Query: 41  PNRGAVRV---LCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSC 100
           PN  A  +   L F AG   F       TAAAQN +A+  VNVGVVLD ES IGKMGLSC
Sbjct: 4   PNPAAAIIPAALYFLAGLFFFT------TAAAQNESAVP-VNVGVVLDMESWIGKMGLSC 63

Query: 101 IDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQAN 160
           IDMSLSEFY+ NP Y TRIVLH ++ G D VVGAAAAA+DLIKN KV AILGPT+SMQAN
Sbjct: 64  IDMSLSEFYSLNPHYHTRIVLHPKDSGRD-VVGAAAAALDLIKNNKVHAILGPTTSMQAN 123

Query: 161 FVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPI 220
           FVIELGQKAHVPIL+FTA+SPALASLRSPYFFRL QNDS QV AIS +VK++ WRQV+PI
Sbjct: 124 FVIELGQKAHVPILTFTASSPALASLRSPYFFRLTQNDSAQVVAISDLVKSYNWRQVIPI 183

Query: 221 YEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHML 280
           YEDDEFGDG+LPYLIDALQ VNARV YRSVI+P AT DQI EELYKL TMQ RVFVVHM+
Sbjct: 184 YEDDEFGDGMLPYLIDALQSVNARVPYRSVIDPEATDDQIKEELYKLMTMQPRVFVVHMV 243

Query: 281 PSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKL 340
           PSLAARLF +A++IGMMSEG+ WILT  T N+L SMDSSVL+SM+GALGVKT+VP S++L
Sbjct: 244 PSLAARLFMMANEIGMMSEGYAWILTDVTTNVLDSMDSSVLNSMEGALGVKTYVPNSLEL 303

Query: 341 DEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLKNP----- 400
           D F +RWKRKFL +NP   +PQLDVFGLWAHDAA+ALAMAVE+ G   F Y  NP     
Sbjct: 304 DGFKIRWKRKFLIENPIPKEPQLDVFGLWAHDAARALAMAVEKTGETEFKYKNNPINESN 363

Query: 401 -NLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVG 460
            NLTDLQ+LGVSENGEK+RD L  T+F+GL GD+ +  GELQS   E+VNVNGN G RVG
Sbjct: 364 NNLTDLQTLGVSENGEKIRDVLLKTRFKGLTGDYRIVKGELQSENFEIVNVNGNGGKRVG 423

Query: 461 FWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITK 520
           FW PE GLTK+ S SGT+ +IWPGDTA  PRGW  P  G RLKIG P K+GYSEFVR+ K
Sbjct: 424 FWNPEKGLTKNLSQSGTKPVIWPGDTATVPRGWVWPVAGKRLKIGFPVKEGYSEFVRVKK 483

Query: 521 NGTDAEGYCTDVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGD 580
           NGT AEGYCTDVF+A +A LPYAVP+DY PF        G+YD+LIM+V+ G +DGAVGD
Sbjct: 484 NGTGAEGYCTDVFDAVMAMLPYAVPYDYVPFAFPNGSSAGSYDDLIMQVYKGTYDGAVGD 543

Query: 581 ITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGF 640
           ITIVANRS YVDFTLPFTESGVSMVVPTQ  SKN+AW+FLKPLTLDLWITSFCFFVFMGF
Sbjct: 544 ITIVANRSNYVDFTLPFTESGVSMVVPTQGYSKNKAWIFLKPLTLDLWITSFCFFVFMGF 603

Query: 641 VVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILT 700
           VVWILEHRINE+FRGPPSHQIGTSLWFSFCTMVFAQRE+L+SNLARFVVVIWFFVVFILT
Sbjct: 604 VVWILEHRINEEFRGPPSHQIGTSLWFSFCTMVFAQRESLVSNLARFVVVIWFFVVFILT 663

Query: 701 QSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPD 760
           QSYTASLTSLLTVQQLQP+ITD+NEL+K QPWVGYQ+GSFV  LL+SVG KNLRPY +P+
Sbjct: 664 QSYTASLTSLLTVQQLQPTITDVNELLKNQPWVGYQDGSFVFELLKSVGIKNLRPYDTPE 723

Query: 761 ELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVA 820
           +L + FK GSSNGG DAAFDEIPY+KLFL KFPDKY M++P YK DGFGFAFP+GSPLV 
Sbjct: 724 QLDEMFKSGSSNGGIDAAFDEIPYVKLFLFKFPDKYIMTEPNYKTDGFGFAFPIGSPLVG 783

Query: 821 DISRQVLNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVA 880
           D+SR VLNVTESEKMN+IQK W G  CNS SSG  V SSRLNL SFWGLFLIAGSAA++A
Sbjct: 784 DVSRAVLNVTESEKMNRIQKTWFGGQCNSVSSGSKVTSSRLNLGSFWGLFLIAGSAAIIA 843

Query: 881 LLIYFAIFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEA 940
           LL+Y  IF +KE HTLR TA +EGSN+    K+RALL+TYD RDLTSHTF+KSN ++ + 
Sbjct: 844 LLVYGFIFFHKEQHTLRHTA-NEGSNNSFRHKIRALLKTYDERDLTSHTFKKSNLVHGDK 903

Query: 941 --HAMDG----ASPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLE 991
              AMDG    ASP SN PPSPSNYSV D SFEF++ S ++SPMN      + +  +   
Sbjct: 904 TIRAMDGSSISASPRSNYPPSPSNYSVHDTSFEFYSESENASPMNHQALEMVVSTSMEAS 963

BLAST of MC08g0462 vs. NCBI nr
Match: XP_004144281.1 (glutamate receptor 2.7 [Cucumis sativus] >KAE8647217.1 hypothetical protein Csa_018932 [Cucumis sativus])

HSP 1 Score: 1352 bits (3498), Expect = 0.0
Identity = 695/963 (72.17%), Postives = 792/963 (82.24%), Query Frame = 0

Query: 49  LCFFAGCVVFLLGTTALTAAAQNATAMAA-VNVGVVLDFESRIGKMGLSCIDMSLSEFYA 108
           L F AG  +F   TTA   AAQNA++    VNVGVVLD ES IGKMGLSCIDMSLSEFY+
Sbjct: 15  LYFLAG--LFFFSTTA---AAQNASSSTVPVNVGVVLDMESWIGKMGLSCIDMSLSEFYS 74

Query: 109 ANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANFVIELGQKAH 168
            NP Y TRIVLH ++ G D VVGAAAAA+DLIKN KV AILGPT+SMQANFVIELGQKAH
Sbjct: 75  LNPHYHTRIVLHPKDSGRD-VVGAAAAALDLIKNNKVHAILGPTTSMQANFVIELGQKAH 134

Query: 169 VPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIYEDDEFGDGI 228
           VPIL+FTA+SPALASLRSPYFFRL QNDS QV AIS +VK++ WRQVVPIYEDDEFGDG+
Sbjct: 135 VPILTFTASSPALASLRSPYFFRLTQNDSAQVVAISDLVKSYSWRQVVPIYEDDEFGDGM 194

Query: 229 LPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLPSLAARLFAI 288
           LPYLIDALQ VNARV YRSVI+PAAT DQI EELYKL TMQ RVFVVHMLPSLAARLF  
Sbjct: 195 LPYLIDALQSVNARVPYRSVIDPAATDDQIKEELYKLMTMQPRVFVVHMLPSLAARLFMK 254

Query: 289 ADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKLDEFTVRWKRK 348
           A++IGMMSEG+ WILT  T N+L S+DSSVL SM+GALGVKT+VPKS++LD F +RWKRK
Sbjct: 255 ANEIGMMSEGYAWILTDGTTNVLDSLDSSVLKSMEGALGVKTYVPKSLELDSFKIRWKRK 314

Query: 349 FLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLKNP------NLTDLQSLG 408
           FL +NP +N+PQLDVFGLWAHDAA+ALAMAVE+ G   F Y  NP        TDLQ+LG
Sbjct: 315 FLIENPIINEPQLDVFGLWAHDAARALAMAVEKTGEREFKYKNNPINESNNKQTDLQTLG 374

Query: 409 VSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTPENGLTK 468
           VSENGEK+RD L  T+F+GL G++ +  GELQS  LE+VNVN + G RVGFW PE GLTK
Sbjct: 375 VSENGEKIRDVLLKTRFKGLTGNYRIVKGELQSDNLEIVNVNEDGGKRVGFWNPEKGLTK 434

Query: 469 DWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITKNGTDAEGYCT 528
           + S SGT+ +IWPGDT A P+GWE P  G RLKIG P K+GY+EFVR+ +NGT AEGYCT
Sbjct: 435 NLSQSGTKPVIWPGDTTAVPKGWEWPVAGKRLKIGFPVKEGYNEFVRVKENGTGAEGYCT 494

Query: 529 DVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGDITIVANRSQY 588
           DVF+A +A LPYAVP+DY PF        G+YD+LI++V+ G +DGAVGDITIVANRS Y
Sbjct: 495 DVFDAVIAKLPYAVPYDYVPFAFPNGSSAGSYDDLIIQVYKGIYDGAVGDITIVANRSNY 554

Query: 589 VDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILEHRIN 648
           VDFTLPFTESGVSMVVPTQ NSKNRAW+FLKPLTL+LWITSFCFFVFMGFVVWILEHRIN
Sbjct: 555 VDFTLPFTESGVSMVVPTQGNSKNRAWIFLKPLTLNLWITSFCFFVFMGFVVWILEHRIN 614

Query: 649 EDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTASLTSL 708
           E+FRGPPSHQIGTSLWFSFCTMVFAQRE+L+SNLARFVVVIWFFVVFILTQSYTASLTSL
Sbjct: 615 EEFRGPPSHQIGTSLWFSFCTMVFAQRESLVSNLARFVVVIWFFVVFILTQSYTASLTSL 674

Query: 709 LTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELHKFFKRGS 768
           LTVQQLQP+ITD+NEL+K QPWVGYQ+GSFV  LL+SVG KNLRPY +P +L + FK GS
Sbjct: 675 LTVQQLQPTITDVNELLKNQPWVGYQDGSFVFELLKSVGIKNLRPYDTPQQLDEMFKSGS 734

Query: 769 SNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQVLNVT 828
           SNGG DAAFDEIPYIKLFL KFPDKY M++P YK DGFGFAFP+GSPLV D+SR VLNVT
Sbjct: 735 SNGGIDAAFDEIPYIKLFLHKFPDKYIMAEPNYKTDGFGFAFPIGSPLVGDVSRAVLNVT 794

Query: 829 ESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFAIFLY 888
           ESEKMNQIQ  W G+ CNS SSG  V SSRL+L SFWGLFLIAGSAA++ALL+Y  IF +
Sbjct: 795 ESEKMNQIQNTWFGDQCNSLSSGSKVTSSRLSLGSFWGLFLIAGSAAIIALLVYGFIFFH 854

Query: 889 KEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSN--NINVEAHAMDG--- 948
           KE HTL RTA D+GSN+ V  K+RA L+TYD RDLTSHTF+KSN  + +     +DG   
Sbjct: 855 KEQHTLHRTA-DQGSNNTVRDKIRAFLKTYDERDLTSHTFKKSNLGHGDKTNRVIDGGSI 914

Query: 949 -ASPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLELTSGEEITEI 991
            ASP SN PP+PSNYSVQD SF+F++ SG++SPMN      + +  +   L +GEEITEI
Sbjct: 915 SASPGSNYPPNPSNYSVQDTSFDFYSESGNASPMNHQALEMVVSTTMDASLGNGEEITEI 970

BLAST of MC08g0462 vs. ExPASy TrEMBL
Match: A0A6J1BT27 (Glutamate receptor OS=Momordica charantia OX=3673 GN=LOC111004591 PE=3 SV=1)

HSP 1 Score: 1914 bits (4957), Expect = 0.0
Identity = 961/962 (99.90%), Postives = 962/962 (100.00%), Query Frame = 0

Query: 31  MHRQDPPKMKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRI 90
           MHRQDPPKMKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVV+DFESRI
Sbjct: 1   MHRQDPPKMKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVMDFESRI 60

Query: 91  GKMGLSCIDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGP 150
           GKMGLSCIDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGP
Sbjct: 61  GKMGLSCIDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGP 120

Query: 151 TSSMQANFVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFG 210
           TSSMQANFVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFG
Sbjct: 121 TSSMQANFVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFG 180

Query: 211 WRQVVPIYEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSR 270
           WRQVVPIYEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSR
Sbjct: 181 WRQVVPIYEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSR 240

Query: 271 VFVVHMLPSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTF 330
           VFVVHMLPSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTF
Sbjct: 241 VFVVHMLPSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTF 300

Query: 331 VPKSMKLDEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK 390
           VPKSMKLDEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK
Sbjct: 301 VPKSMKLDEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK 360

Query: 391 NPNLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRV 450
           NPNLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRV
Sbjct: 361 NPNLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRV 420

Query: 451 GFWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRIT 510
           GFWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRIT
Sbjct: 421 GFWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRIT 480

Query: 511 KNGTDAEGYCTDVFEAALAGLPYAVPFDYFPFDGAYDELIMKVHDGFFDGAVGDITIVAN 570
           KNGTDAEGYCTDVFEAALAGLPYAVPFDYFPFDGAYDELIMKVHDGFFDGAVGDITIVAN
Sbjct: 481 KNGTDAEGYCTDVFEAALAGLPYAVPFDYFPFDGAYDELIMKVHDGFFDGAVGDITIVAN 540

Query: 571 RSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILE 630
           RSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILE
Sbjct: 541 RSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILE 600

Query: 631 HRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTAS 690
           HRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTAS
Sbjct: 601 HRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTAS 660

Query: 691 LTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELHKFF 750
           LTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELHKFF
Sbjct: 661 LTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELHKFF 720

Query: 751 KRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQV 810
           KRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQV
Sbjct: 721 KRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQV 780

Query: 811 LNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFA 870
           LNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFA
Sbjct: 781 LNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFA 840

Query: 871 IFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGA 930
           IFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGA
Sbjct: 841 IFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGA 900

Query: 931 SPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLELTSGEEITEIIV 990
           SPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLELTSGEEITEIIV
Sbjct: 901 SPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLELTSGEEITEIIV 960

Query: 991 NN 992
           NN
Sbjct: 961 NN 962

BLAST of MC08g0462 vs. ExPASy TrEMBL
Match: A0A1S3CMI1 (Glutamate receptor OS=Cucumis melo OX=3656 GN=LOC103502645 PE=3 SV=1)

HSP 1 Score: 1363 bits (3528), Expect = 0.0
Identity = 702/973 (72.15%), Postives = 794/973 (81.60%), Query Frame = 0

Query: 41  PNRGAVRV---LCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSC 100
           PN  A  +   L F AG   F       TAAAQN +A+  VNVGVVLD ES IGKMGLSC
Sbjct: 4   PNPAAAIIPAALYFLAGLFFFT------TAAAQNESAVP-VNVGVVLDMESWIGKMGLSC 63

Query: 101 IDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQAN 160
           IDMSLSEFY+ NP Y TRIVLH ++ G D VVGAAAAA+DLIKN KV AILGPT+SMQAN
Sbjct: 64  IDMSLSEFYSLNPHYHTRIVLHPKDSGRD-VVGAAAAALDLIKNNKVHAILGPTTSMQAN 123

Query: 161 FVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPI 220
           FVIELGQKAHVPIL+FTA+SPALASLRSPYFFRL QNDS QV AIS +VK++ WRQV+PI
Sbjct: 124 FVIELGQKAHVPILTFTASSPALASLRSPYFFRLTQNDSAQVVAISDLVKSYNWRQVIPI 183

Query: 221 YEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHML 280
           YEDDEFGDG+LPYLIDALQ VNARV YRSVI+P AT DQI EELYKL TMQ RVFVVHM+
Sbjct: 184 YEDDEFGDGMLPYLIDALQSVNARVPYRSVIDPEATDDQIKEELYKLMTMQPRVFVVHMV 243

Query: 281 PSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKL 340
           PSLAARLF +A++IGMMSEG+ WILT  T N+L SMDSSVL+SM+GALGVKT+VP S++L
Sbjct: 244 PSLAARLFMMANEIGMMSEGYAWILTDVTTNVLDSMDSSVLNSMEGALGVKTYVPNSLEL 303

Query: 341 DEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLKNP----- 400
           D F +RWKRKFL +NP   +PQLDVFGLWAHDAA+ALAMAVE+ G   F Y  NP     
Sbjct: 304 DGFKIRWKRKFLIENPIPKEPQLDVFGLWAHDAARALAMAVEKTGETEFKYKNNPINESN 363

Query: 401 -NLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVG 460
            NLTDLQ+LGVSENGEK+RD L  T+F+GL GD+ +  GELQS   E+VNVNGN G RVG
Sbjct: 364 NNLTDLQTLGVSENGEKIRDVLLKTRFKGLTGDYRIVKGELQSENFEIVNVNGNGGKRVG 423

Query: 461 FWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITK 520
           FW PE GLTK+ S SGT+ +IWPGDTA  PRGW  P  G RLKIG P K+GYSEFVR+ K
Sbjct: 424 FWNPEKGLTKNLSQSGTKPVIWPGDTATVPRGWVWPVAGKRLKIGFPAKEGYSEFVRVKK 483

Query: 521 NGTDAEGYCTDVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGD 580
           NGT AEGYCTDVF+A +A LPYAVP+DY PF        G+YD+LIM+V+ G +DGAVGD
Sbjct: 484 NGTGAEGYCTDVFDAVMAMLPYAVPYDYVPFAFPNGSSAGSYDDLIMQVYKGIYDGAVGD 543

Query: 581 ITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGF 640
           ITIVANRS YVDFTLPFTESGVSMVVPTQ  SKN+AW+FLKPLTLDLWITSFCFFVFMGF
Sbjct: 544 ITIVANRSNYVDFTLPFTESGVSMVVPTQGYSKNKAWIFLKPLTLDLWITSFCFFVFMGF 603

Query: 641 VVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILT 700
           VVWILEHRINE+FRGPPSHQIGTSLWFSFCTMVFAQRE+L+SNLARFVVVIWFFVVFILT
Sbjct: 604 VVWILEHRINEEFRGPPSHQIGTSLWFSFCTMVFAQRESLVSNLARFVVVIWFFVVFILT 663

Query: 701 QSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPD 760
           QSYTASLTSLLTVQQLQP+ITD+NEL+K QPWVGYQ+GSFV  LL+SVG KNLRPY +P+
Sbjct: 664 QSYTASLTSLLTVQQLQPTITDVNELLKNQPWVGYQDGSFVFELLKSVGIKNLRPYDTPE 723

Query: 761 ELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVA 820
           +L + FK GSSNGG DAAFDEIPY+KLFL KFPDKY M++P YK DGFGFAFP+GSPLV 
Sbjct: 724 QLDEMFKSGSSNGGIDAAFDEIPYVKLFLFKFPDKYIMAEPNYKTDGFGFAFPIGSPLVG 783

Query: 821 DISRQVLNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVA 880
           D+SR VLNVTESEKMN+IQK W G  CNS SSG  V SSRLNL SFWGLFLIAGSAA++A
Sbjct: 784 DVSRAVLNVTESEKMNRIQKTWFGGQCNSVSSGSKVTSSRLNLGSFWGLFLIAGSAAIIA 843

Query: 881 LLIYFAIFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEA 940
           LL+Y  IF +KE HTLR TA +EGSN+    K+RALL+TYD RDLTSHTF+KSN ++ + 
Sbjct: 844 LLVYGFIFFHKEQHTLRHTA-NEGSNNSFRHKIRALLKTYDERDLTSHTFKKSNLVHGDK 903

Query: 941 --HAMDG----ASPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLE 991
              AMDG    ASP SN PPSPSNYSV D SFEF++ SG++SPMN      + +  +   
Sbjct: 904 TIRAMDGSSISASPRSNYPPSPSNYSVHDTSFEFYSESGNASPMNHQALEMVVSTSMEAS 963

BLAST of MC08g0462 vs. ExPASy TrEMBL
Match: A0A5A7T4U2 (Glutamate receptor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00420 PE=3 SV=1)

HSP 1 Score: 1358 bits (3516), Expect = 0.0
Identity = 701/973 (72.05%), Postives = 793/973 (81.50%), Query Frame = 0

Query: 41  PNRGAVRV---LCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSC 100
           PN  A  +   L F AG   F       TAAAQN +A+  VNVGVVLD ES IGKMGLSC
Sbjct: 4   PNPAAAIIPAALYFLAGLFFFT------TAAAQNESAVP-VNVGVVLDMESWIGKMGLSC 63

Query: 101 IDMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQAN 160
           IDMSLSEFY+ NP Y TRIVLH ++ G D VVGAAAAA+DLIKN KV AILGPT+SMQAN
Sbjct: 64  IDMSLSEFYSLNPHYHTRIVLHPKDSGRD-VVGAAAAALDLIKNNKVHAILGPTTSMQAN 123

Query: 161 FVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPI 220
           FVIELGQKAHVPIL+FTA+SPALASLRSPYFFRL QNDS QV AIS +VK++ WRQV+PI
Sbjct: 124 FVIELGQKAHVPILTFTASSPALASLRSPYFFRLTQNDSAQVVAISDLVKSYNWRQVIPI 183

Query: 221 YEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHML 280
           YEDDEFGDG+LPYLIDALQ VNARV YRSVI+P AT DQI EELYKL TMQ RVFVVHM+
Sbjct: 184 YEDDEFGDGMLPYLIDALQSVNARVPYRSVIDPEATDDQIKEELYKLMTMQPRVFVVHMV 243

Query: 281 PSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKL 340
           PSLAARLF +A++IGMMSEG+ WILT  T N+L SMDSSVL+SM+GALGVKT+VP S++L
Sbjct: 244 PSLAARLFMMANEIGMMSEGYAWILTDVTTNVLDSMDSSVLNSMEGALGVKTYVPNSLEL 303

Query: 341 DEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLKNP----- 400
           D F +RWKRKFL +NP   +PQLDVFGLWAHDAA+ALAMAVE+ G   F Y  NP     
Sbjct: 304 DGFKIRWKRKFLIENPIPKEPQLDVFGLWAHDAARALAMAVEKTGETEFKYKNNPINESN 363

Query: 401 -NLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVG 460
            NLTDLQ+LGVSENGEK+RD L  T+F+GL GD+ +  GELQS   E+VNVNGN G RVG
Sbjct: 364 NNLTDLQTLGVSENGEKIRDVLLKTRFKGLTGDYRIVKGELQSENFEIVNVNGNGGKRVG 423

Query: 461 FWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITK 520
           FW PE GLTK+ S SGT+ +IWPGDTA  PRGW  P  G RLKIG P K+GYSEFVR+ K
Sbjct: 424 FWNPEKGLTKNLSQSGTKPVIWPGDTATVPRGWVWPVAGKRLKIGFPVKEGYSEFVRVKK 483

Query: 521 NGTDAEGYCTDVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGD 580
           NGT AEGYCTDVF+A +A LPYAVP+DY PF        G+YD+LIM+V+ G +DGAVGD
Sbjct: 484 NGTGAEGYCTDVFDAVMAMLPYAVPYDYVPFAFPNGSSAGSYDDLIMQVYKGTYDGAVGD 543

Query: 581 ITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGF 640
           ITIVANRS YVDFTLPFTESGVSMVVPTQ  SKN+AW+FLKPLTLDLWITSFCFFVFMGF
Sbjct: 544 ITIVANRSNYVDFTLPFTESGVSMVVPTQGYSKNKAWIFLKPLTLDLWITSFCFFVFMGF 603

Query: 641 VVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILT 700
           VVWILEHRINE+FRGPPSHQIGTSLWFSFCTMVFAQRE+L+SNLARFVVVIWFFVVFILT
Sbjct: 604 VVWILEHRINEEFRGPPSHQIGTSLWFSFCTMVFAQRESLVSNLARFVVVIWFFVVFILT 663

Query: 701 QSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPD 760
           QSYTASLTSLLTVQQLQP+ITD+NEL+K QPWVGYQ+GSFV  LL+SVG KNLRPY +P+
Sbjct: 664 QSYTASLTSLLTVQQLQPTITDVNELLKNQPWVGYQDGSFVFELLKSVGIKNLRPYDTPE 723

Query: 761 ELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVA 820
           +L + FK GSSNGG DAAFDEIPY+KLFL KFPDKY M++P YK DGFGFAFP+GSPLV 
Sbjct: 724 QLDEMFKSGSSNGGIDAAFDEIPYVKLFLFKFPDKYIMTEPNYKTDGFGFAFPIGSPLVG 783

Query: 821 DISRQVLNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVA 880
           D+SR VLNVTESEKMN+IQK W G  CNS SSG  V SSRLNL SFWGLFLIAGSAA++A
Sbjct: 784 DVSRAVLNVTESEKMNRIQKTWFGGQCNSVSSGSKVTSSRLNLGSFWGLFLIAGSAAIIA 843

Query: 881 LLIYFAIFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEA 940
           LL+Y  IF +KE HTLR TA +EGSN+    K+RALL+TYD RDLTSHTF+KSN ++ + 
Sbjct: 844 LLVYGFIFFHKEQHTLRHTA-NEGSNNSFRHKIRALLKTYDERDLTSHTFKKSNLVHGDK 903

Query: 941 --HAMDG----ASPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQEVGLE 991
              AMDG    ASP SN PPSPSNYSV D SFEF++ S ++SPMN      + +  +   
Sbjct: 904 TIRAMDGSSISASPRSNYPPSPSNYSVHDTSFEFYSESENASPMNHQALEMVVSTSMEAS 963

BLAST of MC08g0462 vs. ExPASy TrEMBL
Match: A0A6J1KPY2 (Glutamate receptor OS=Cucurbita maxima OX=3661 GN=LOC111496545 PE=3 SV=1)

HSP 1 Score: 1252 bits (3240), Expect = 0.0
Identity = 651/931 (69.92%), Postives = 748/931 (80.34%), Query Frame = 0

Query: 39  MKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCI 98
           MKPN+G  R L F AGCV+ +    A    A+N      V+VGVVLD ES +GKMGLSCI
Sbjct: 1   MKPNQGIRRALWFIAGCVLLV----ATAGEARN------VSVGVVLDMESWVGKMGLSCI 60

Query: 99  DMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANF 158
            MSLSEFY ANP Y TRIVLH ++  +D VVGAAAAA+DLIKN KVQAILGPT+SMQ NF
Sbjct: 61  HMSLSEFYQANPHYNTRIVLHPKDSASD-VVGAAAAALDLIKNNKVQAILGPTTSMQTNF 120

Query: 159 VIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIY 218
           VI+L  KAHVPIL+FTA+SP LAS RSPYFFRL Q DS QVAAISA+VK + WRQVV IY
Sbjct: 121 VIKLAHKAHVPILTFTASSPRLASYRSPYFFRLTQTDSAQVAAISALVKAYNWRQVVLIY 180

Query: 219 EDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLP 278
           +DDEFGDG+LPYLIDALQDVNARV YRSVI+P AT DQIGEELYKL TM +RVF+VHM P
Sbjct: 181 QDDEFGDGMLPYLIDALQDVNARVPYRSVIDPTATEDQIGEELYKLMTMPTRVFLVHMQP 240

Query: 279 SLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKLD 338
           SLA RLFA A+KIGMM EG+ WILT   ANLL SM SSVL+SM+GALGVKT+VPKSM+L 
Sbjct: 241 SLAIRLFAKANKIGMMREGYAWILTDVIANLLDSMSSSVLNSMEGALGVKTYVPKSMELA 300

Query: 339 EFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLKNP----NL 398
            F ++WKR+F+ +N  L DP LD+FGLWA+DAA+ALAMA+E+ G  NFT+ +NP    NL
Sbjct: 301 RFKIKWKREFVMENSVLTDPHLDIFGLWAYDAARALAMAIEKTGAKNFTF-ENPKGSENL 360

Query: 399 TDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWT 458
           TDL++LGVS NGEK+ + LS T+F GL G++ + NG+LQSA  E+VNVN N GNRVGFW 
Sbjct: 361 TDLETLGVSRNGEKIVEGLSKTQFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWN 420

Query: 459 PENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITKNGT 518
           PE GL  +        +IWPG T A P+GWE PT G RLKIGVP K+GYSEFVR+  NG 
Sbjct: 421 PEKGLLGN-----NMIVIWPGTTEAVPKGWEFPTAGKRLKIGVPVKEGYSEFVRV--NGK 480

Query: 519 DAEGYCTDVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGDITI 578
           + EGYC DVF+A +  LPYA+PFDY PF        G+Y++LIM+V+ G +DGAVGD+TI
Sbjct: 481 EVEGYCRDVFDAVIEALPYALPFDYIPFALPNGSSAGSYNDLIMQVNRGVYDGAVGDLTI 540

Query: 579 VANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVW 638
           VANRS+YVDFTLPFTESGVSM+VPTQ NSKNRAW+FLKPLTLDLWITSFCFFVFMGFVVW
Sbjct: 541 VANRSKYVDFTLPFTESGVSMIVPTQPNSKNRAWLFLKPLTLDLWITSFCFFVFMGFVVW 600

Query: 639 ILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSY 698
           ILEHRIN+DFRGPP+HQIGTSLW+SFCTMVFAQRETLISNLARFVVVIWFFVVF+LTQSY
Sbjct: 601 ILEHRINQDFRGPPTHQIGTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSY 660

Query: 699 TASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPYASPDELH 758
           TASLTSLLTVQQLQP+IT+INEL+K QPWVGYQ+GSFV GLL SVG KNL+ Y SP+EL 
Sbjct: 661 TASLTSLLTVQQLQPTITNINELLKTQPWVGYQDGSFVWGLLTSVGIKNLKSYGSPEELD 720

Query: 759 KFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADIS 818
           +  + GSSNGG DAAFDE+PY+KLFLS F DKYTM DP YK DGFGFAFP+GSPLVADIS
Sbjct: 721 ELLRLGSSNGGIDAAFDEMPYVKLFLSMFHDKYTMGDPNYKTDGFGFAFPIGSPLVADIS 780

Query: 819 RQVLNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLI 878
           R VLNVTESEKMNQ+Q+KW     NS SS   +  SRLNLSSFWGLFLIAG+AA+ ALLI
Sbjct: 781 RAVLNVTESEKMNQLQRKWFKNEDNSWSSISKITPSRLNLSSFWGLFLIAGTAAITALLI 840

Query: 879 YFAIFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINV---EA 938
           YF IFLYKE HTL   A +E SNS +  K+RALLR YD  DLTSH F+KSN+  +   + 
Sbjct: 841 YFIIFLYKEQHTLSH-AVNEDSNSSIGSKIRALLRIYDKIDLTSHMFKKSNHSQMADNKI 900

Query: 939 HAMDG----ASPSSNCPPSPSNYSVQDASFE 951
           HA+ G    ASPSSN PPSPSNYS  D+  E
Sbjct: 901 HAIYGDSVGASPSSNYPPSPSNYSDHDSDSE 911

BLAST of MC08g0462 vs. ExPASy TrEMBL
Match: A0A1S4E4V8 (Glutamate receptor OS=Cucumis melo OX=3656 GN=LOC103502645 PE=3 SV=1)

HSP 1 Score: 1246 bits (3225), Expect = 0.0
Identity = 627/857 (73.16%), Postives = 711/857 (82.96%), Query Frame = 0

Query: 154 MQANFVIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQ 213
           MQANFVIELGQKAHVPIL+FTA+SPALASLRSPYFFRL QNDS QV AIS +VK++ WRQ
Sbjct: 1   MQANFVIELGQKAHVPILTFTASSPALASLRSPYFFRLTQNDSAQVVAISDLVKSYNWRQ 60

Query: 214 VVPIYEDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFV 273
           V+PIYEDDEFGDG+LPYLIDALQ VNARV YRSVI+P AT DQI EELYKL TMQ RVFV
Sbjct: 61  VIPIYEDDEFGDGMLPYLIDALQSVNARVPYRSVIDPEATDDQIKEELYKLMTMQPRVFV 120

Query: 274 VHMLPSLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPK 333
           VHM+PSLAARLF +A++IGMMSEG+ WILT  T N+L SMDSSVL+SM+GALGVKT+VP 
Sbjct: 121 VHMVPSLAARLFMMANEIGMMSEGYAWILTDVTTNVLDSMDSSVLNSMEGALGVKTYVPN 180

Query: 334 SMKLDEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLKNP- 393
           S++LD F +RWKRKFL +NP   +PQLDVFGLWAHDAA+ALAMAVE+ G   F Y  NP 
Sbjct: 181 SLELDGFKIRWKRKFLIENPIPKEPQLDVFGLWAHDAARALAMAVEKTGETEFKYKNNPI 240

Query: 394 -----NLTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWG 453
                NLTDLQ+LGVSENGEK+RD L  T+F+GL GD+ +  GELQS   E+VNVNGN G
Sbjct: 241 NESNNNLTDLQTLGVSENGEKIRDVLLKTRFKGLTGDYRIVKGELQSENFEIVNVNGNGG 300

Query: 454 NRVGFWTPENGLTKDWSASGTRSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFV 513
            RVGFW PE GLTK+ S SGT+ +IWPGDTA  PRGW  P  G RLKIG P K+GYSEFV
Sbjct: 301 KRVGFWNPEKGLTKNLSQSGTKPVIWPGDTATVPRGWVWPVAGKRLKIGFPAKEGYSEFV 360

Query: 514 RITKNGTDAEGYCTDVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDG 573
           R+ KNGT AEGYCTDVF+A +A LPYAVP+DY PF        G+YD+LIM+V+ G +DG
Sbjct: 361 RVKKNGTGAEGYCTDVFDAVMAMLPYAVPYDYVPFAFPNGSSAGSYDDLIMQVYKGIYDG 420

Query: 574 AVGDITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFV 633
           AVGDITIVANRS YVDFTLPFTESGVSMVVPTQ  SKN+AW+FLKPLTLDLWITSFCFFV
Sbjct: 421 AVGDITIVANRSNYVDFTLPFTESGVSMVVPTQGYSKNKAWIFLKPLTLDLWITSFCFFV 480

Query: 634 FMGFVVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVV 693
           FMGFVVWILEHRINE+FRGPPSHQIGTSLWFSFCTMVFAQRE+L+SNLARFVVVIWFFVV
Sbjct: 481 FMGFVVWILEHRINEEFRGPPSHQIGTSLWFSFCTMVFAQRESLVSNLARFVVVIWFFVV 540

Query: 694 FILTQSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFKNLRPY 753
           FILTQSYTASLTSLLTVQQLQP+ITD+NEL+K QPWVGYQ+GSFV  LL+SVG KNLRPY
Sbjct: 541 FILTQSYTASLTSLLTVQQLQPTITDVNELLKNQPWVGYQDGSFVFELLKSVGIKNLRPY 600

Query: 754 ASPDELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGS 813
            +P++L + FK GSSNGG DAAFDEIPY+KLFL KFPDKY M++P YK DGFGFAFP+GS
Sbjct: 601 DTPEQLDEMFKSGSSNGGIDAAFDEIPYVKLFLFKFPDKYIMAEPNYKTDGFGFAFPIGS 660

Query: 814 PLVADISRQVLNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSA 873
           PLV D+SR VLNVTESEKMN+IQK W G  CNS SSG  V SSRLNL SFWGLFLIAGSA
Sbjct: 661 PLVGDVSRAVLNVTESEKMNRIQKTWFGGQCNSVSSGSKVTSSRLNLGSFWGLFLIAGSA 720

Query: 874 AVVALLIYFAIFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNI 933
           A++ALL+Y  IF +KE HTLR TA +EGSN+    K+RALL+TYD RDLTSHTF+KSN +
Sbjct: 721 AIIALLVYGFIFFHKEQHTLRHTA-NEGSNNSFRHKIRALLKTYDERDLTSHTFKKSNLV 780

Query: 934 NVEA--HAMDG----ASPSSNCPPSPSNYSVQDASFEFFNGSGDSSPMNRNQTPPLHNQE 991
           + +    AMDG    ASP SN PPSPSNYSV D SFEF++ SG++SPMN      + +  
Sbjct: 781 HGDKTIRAMDGSSISASPRSNYPPSPSNYSVHDTSFEFYSESGNASPMNHQALEMVVSTS 840

BLAST of MC08g0462 vs. TAIR 10
Match: AT2G29110.1 (glutamate receptor 2.8 )

HSP 1 Score: 825.1 bits (2130), Expect = 6.1e-239
Identity = 451/942 (47.88%), Postives = 607/942 (64.44%), Query Frame = 0

Query: 39  MKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCI 98
           M P +     L +F  C+  LL         QN   ++ + VGVVLD  +   K+ L+ I
Sbjct: 1   MNPKKNNNTFLSYFV-CLFLLLE----VGLGQN--QISEIKVGVVLDLNTTFSKICLTSI 60

Query: 99  DMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANF 158
           +++LS+FY  +P+Y+TR+ LH R+    D V A+AAA+DLI+N++V AI+GP  SMQA F
Sbjct: 61  NLALSDFYKDHPNYRTRLALHVRD-SMKDTVQASAAALDLIQNEQVSAIIGPIDSMQAKF 120

Query: 159 VIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIY 218
           +I+L  K  VP +SF+ATSP L S++S YF R   +DS QV AI+AI ++FGWR VV IY
Sbjct: 121 MIKLANKTQVPTISFSATSPLLTSIKSDYFVRGTIDDSYQVKAIAAIFESFGWRSVVAIY 180

Query: 219 EDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLP 278
            D+E G+GI+PYL DALQDV      RSVI   A  DQI +ELYKL T Q+RVFVVHM  
Sbjct: 181 VDNELGEGIMPYLFDALQDVQVD---RSVIPSEANDDQILKELYKLMTRQTRVFVVHMAS 240

Query: 279 SLAARLFAIADKIGMMSEGFVWILTGATANLLQSM-DSSVLHSMDGALGVKTFVPKSMKL 338
            LA+R+F  A +IGMM EG+VW++T    ++++ +     L+++DG LGV++ VPKS  L
Sbjct: 241 RLASRIFEKATEIGMMEEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKSKGL 300

Query: 339 DEFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYL----KNPN 398
           ++F +RWKR F  +NP L D  L +FGLWA+D+  ALAMAVE+   ++F Y      + N
Sbjct: 301 EDFRLRWKRNFKKENPWLRD-DLSIFGLWAYDSTTALAMAVEKTNISSFPYNNASGSSNN 360

Query: 399 LTDLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFW 458
           +TDL +L VS  G  L + LS  +F GLAG F + + +L+S   E++N  GN    VGFW
Sbjct: 361 MTDLGTLHVSRYGPSLLEALSEIRFNGLAGRFNLIDRQLESPKFEIINFVGNEERIVGFW 420

Query: 459 TPENGL-----TKDWSASGTR--SIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEF 518
           TP NGL      K  S +G R   +IWPG +   P+GWE+PT G ++K+GVP K G+  F
Sbjct: 421 TPSNGLVNVNSNKTTSFTGERFGPLIWPGKSTIVPKGWEIPTNGKKIKVGVPVKKGFFNF 480

Query: 519 VRI----TKNGTDAEGYCTDVFEAALAGLPYAVPFDYFPF---DGAYDELIMKVHDGFFD 578
           V +      N T  +GY  D+FEAAL  LPY+V   Y+ F   D  YD+L+ KV +G  D
Sbjct: 481 VEVITDPITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFESPDDDYDDLVYKVDNGTLD 540

Query: 579 GAVGDITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFF 638
             VGD+TI A RS Y DFTLP+TESGVSM+VP + N     WVFLKP  LDLW+T+ CFF
Sbjct: 541 AVVGDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVTTACFF 600

Query: 639 VFMGFVVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFV 698
           V +GFVVW+ EHR+N DFRGPP HQIGTS WFSF TMVFA RE ++SNLARFVVV+W FV
Sbjct: 601 VLIGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVVVWCFV 660

Query: 699 VFILTQSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGF--KNL 758
           V +LTQSYTA+LTS LTVQ+ QP+  ++ +L+K   +VGYQ G+FV   L   GF    L
Sbjct: 661 VLVLTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKEGFNVSKL 720

Query: 759 RPYASPDELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFP 818
           +P+ S +E H       SNG   AAFDE+ Y++  LS++  KY + +P +K  GFGFAFP
Sbjct: 721 KPFGSSEECHALL----SNGSISAAFDEVAYLRAILSQYCSKYAIVEPTFKTAGFGFAFP 780

Query: 819 MGSPLVADISRQVLNVTESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIA 878
             SPL  D+S+ +LNVT+ ++M  I+ KW  +  + P     + S+RL+L SFWGLFLIA
Sbjct: 781 RNSPLTGDVSKAILNVTQGDEMQHIENKWFMKQNDCPDPKTALSSNRLSLRSFWGLFLIA 840

Query: 879 GSAAVVALLIYFAIFLYKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKS 938
           G A+ +ALLI+  +FLY+  HTL     D+  +S + RKL +L R +D +D+ SHTF+ S
Sbjct: 841 GIASFLALLIFVFLFLYENRHTL----CDDSEDS-IWRKLTSLFRNFDEKDIKSHTFKSS 900

Query: 939 NNINVEAHAMDGASPSSNCPPSPSNYSV--------QDASFE 952
              +V       +SP +   PSPS   +        QD +FE
Sbjct: 901 AVHHV-------SSPMTQYIPSPSTLQIAPRPHSPSQDRAFE 914

BLAST of MC08g0462 vs. TAIR 10
Match: AT2G29120.1 (glutamate receptor 2.7 )

HSP 1 Score: 811.2 bits (2094), Expect = 9.1e-235
Identity = 444/915 (48.52%), Postives = 596/915 (65.14%), Query Frame = 0

Query: 56  VVFLLGTTALTAA-AQNATAMAAVNVGVVLDFESRIGKMGLSCIDMSLSEFYAANPDYKT 115
           V+F+ G   +     QN T    + VGVVLD  +   K+ L+ I++SLS+FY  + DY T
Sbjct: 18  VLFVCGFVLMEGCLGQNQT--TEIKVGVVLDLHTSFSKLCLTSINISLSDFYKYHSDYTT 77

Query: 116 RIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANFVIELGQKAHVPILSFT 175
           R+ +H R+   +DVV A++AA+DLIKN++V AI+GP +SMQA F+I L  K+ VP ++F+
Sbjct: 78  RLAIHIRD-SMEDVVQASSAALDLIKNEQVSAIIGPRTSMQAEFMIRLADKSQVPTITFS 137

Query: 176 ATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIYEDDEFGDGILPYLIDA 235
           AT P L S+ SPYF R   +DS QV AI+AIVK+FGWR VV IY D+EFG+GILP L DA
Sbjct: 138 ATCPLLTSINSPYFVRATLDDSSQVKAIAAIVKSFGWRNVVAIYVDNEFGEGILPLLTDA 197

Query: 236 LQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLPSLAARLFAIADKIGMM 295
           LQDV A V  R +I   A  DQI +ELYKL TMQ+RVFVVHM P+L  R F  A +IGMM
Sbjct: 198 LQDVQAFVVNRCLIPQEANDDQILKELYKLMTMQTRVFVVHMPPTLGFRFFQKAREIGMM 257

Query: 296 SEGFVWILTGATANLLQSMD-SSVLHSMDGALGVKTFVPKSMKLDEFTVRWKRKFLSQNP 355
            EG+VW+LT    NLL+S +  S L +M G LGV++ +PKS KL  F +RW++ F  +  
Sbjct: 258 EEGYVWLLTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSKKLKNFRLRWEKMFPKKG- 317

Query: 356 TLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTY----LKNPNLTDLQSLGVSENGEKL 415
             ND ++++F L A+D+  ALAMAVE+    +  Y        N T+L +LGVS  G  L
Sbjct: 318 --NDEEMNIFALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSL 377

Query: 416 RDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTPENGLTKDWSASGTR 475
              LS  +F GLAG+F + NG+L+S+  +V+N+ G+    +G W P NG+    S + T 
Sbjct: 378 LKALSNVRFNGLAGEFELINGQLESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTS 437

Query: 476 -------SIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRI----TKNGTDAEG 535
                   +IWPG +   P+GW++PT G  L++G+P K G+ EFV        N     G
Sbjct: 438 VLGERLGPVIWPGKSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTG 497

Query: 536 YCTDVFEAALAGLPYAVPFDYFPF---DGAYDELIMKVHDGFFDGAVGDITIVANRSQYV 595
           YC ++FEA L  LPY+V   Y  F   D  YDE++ +V+ G +D  VGD+TIVANRS YV
Sbjct: 498 YCIEIFEAVLKKLPYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYV 557

Query: 596 DFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILEHRINE 655
           DFTLP+TESGVSM+VP + N     WVFL+P +LDLW+T+ CFFVF+GF+VWILEHR+N 
Sbjct: 558 DFTLPYTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNT 617

Query: 656 DFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTASLTSLL 715
           DFRGPP HQIGTS WF+F TM FA RE ++SNLARFVV++W FVV +L QSYTA+LTS  
Sbjct: 618 DFRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFF 677

Query: 716 TVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGF--KNLRPYASPDELHKFFKRG 775
           TV+ LQP++T+  +L+K    +GYQ G+FV  LL+S GF    L+P+ S  E  + F   
Sbjct: 678 TVKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQGFDESQLKPFGSAVECDELF--- 737

Query: 776 SSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQVLNV 835
            SNG   A+FDE+ YIK+ LS+   KYTM +P +K  GFGF FP  SPL  D+SR +LNV
Sbjct: 738 -SNGTITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNV 797

Query: 836 TESEKMNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFAIFL 895
           T+ E+M  I+ KW  +  N P    ++ S+ L+LSSFWGLFLIAG A+ +ALLI+ A FL
Sbjct: 798 TQGEEMQHIENKWFKKPNNCPDLNTSLSSNHLSLSSFWGLFLIAGIASFLALLIFVANFL 857

Query: 896 YKEHHTLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGAS-- 944
           Y+  HTL     D+  NS    KL+ L+R +D +D+ SH F+++   NV +    G+S  
Sbjct: 858 YEHKHTL----FDDSENS-FRGKLKFLVRNFDEKDIKSHMFKENAVHNVSSPITQGSSSP 915

BLAST of MC08g0462 vs. TAIR 10
Match: AT2G29100.1 (glutamate receptor 2.9 )

HSP 1 Score: 807.7 bits (2085), Expect = 1.0e-233
Identity = 440/944 (46.61%), Postives = 614/944 (65.04%), Query Frame = 0

Query: 55  CVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCIDMSLSEFYAANPDYKT 114
           C   L+G        QN T  + + VGVVLD  +   K+ L+ I M++S+FYA +P+Y T
Sbjct: 13  CGFLLMG----VGLGQNQT--SEIKVGVVLDLNTTFSKICLTSIKMAVSDFYADHPNYLT 72

Query: 115 RIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANFVIELGQKAHVPILSFT 174
           R+ LH R+   +D V A+AAA+DLIK ++V AI+GP +SMQA+F+I+L  K  VP ++F+
Sbjct: 73  RLTLHVRD-SMEDTVQASAAALDLIKTEQVSAIIGPINSMQADFMIKLANKTQVPTITFS 132

Query: 175 ATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIYEDDEFGDGILPYLIDA 234
           ATSP L S++SPYF R   +DS QV AI++I K F WR+VV IY D+EFG+G +P+L DA
Sbjct: 133 ATSPLLTSIKSPYFVRATIDDSSQVRAIASIFKFFRWRRVVAIYVDNEFGEGFMPFLFDA 192

Query: 235 LQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLPSLAARLFAIADKIGMM 294
           LQDV  +   RSVI P A  D+I +EL KL   Q+RVFVVHM  SLA R+F IA  IGMM
Sbjct: 193 LQDVEVK---RSVIPPEAIDDEIQKELRKLMERQARVFVVHMESSLALRVFQIARDIGMM 252

Query: 295 SEGFVWILTGATANLLQSMDSS-VLHSMDGALGVKTFVPKSMKLDEFTVRWKRKFLSQNP 354
            EG+VW++T    ++++ +++   L++++G LGV++ VPKS +L +F +RWKR F  +NP
Sbjct: 253 EEGYVWLMTNGMTHMMRHINNGRSLNTIEGVLGVRSHVPKSKELGDFRLRWKRTFEKENP 312

Query: 355 TLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTY----LKNPNLTDLQSLGVSENGEKL 414
           ++ D  L+VF LWA+D+  ALA AVE+    +  Y      + N TDL ++GVS  G  L
Sbjct: 313 SMRD-DLNVFALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSL 372

Query: 415 RDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTPENGLTKDWSASGTR 474
           +   S  +F GLAG+F + +G+LQS   E++N  GN    +GFWTP +GL  D ++S  +
Sbjct: 373 QKAFSEVRFNGLAGEFKLIDGQLQSPKFEIINFVGNEERIIGFWTPRDGL-MDATSSNKK 432

Query: 475 S---IIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRIT----KNGTDAEGYCTD 534
           +   +IWPG +   P+GWE+P  G +L++GVP K G+ +FV++T     N     GY  +
Sbjct: 433 TLGPVIWPGKSKIVPKGWEIP--GKKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIE 492

Query: 535 VFEAALAGLPYAVPFDYFPFDGA--YDELIMKVHDGFFDGAVGDITIVANRSQYVDFTLP 594
           +FEAAL  LPY V  +Y  F+    Y+ L+ +V+D  +D  VGDITI ANRS Y DFTLP
Sbjct: 493 IFEAALKELPYLVIPEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLP 552

Query: 595 FTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWILEHRINEDFRGP 654
           FTESGVSM+VP + N     WVFL+P +L+LW+T+ CFFVF+GFVVW+ EHR+N DFRGP
Sbjct: 553 FTESGVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGP 612

Query: 655 PSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYTASLTSLLTVQQL 714
           P +QIGTSLWFSF TMVFA RE ++SNLARFVVV+W FVV +LTQSYTASLTS LTVQ L
Sbjct: 613 PQYQIGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSL 672

Query: 715 QPSITDINELMKRQPWVGYQEGSFVLGLLRSVGF--KNLRPYASPDELHKFFKRGSSNGG 774
           QP++T++N+L+K +  VGYQ G+FV  +L  +GF    L+P+ S  +      +G S  G
Sbjct: 673 QPTVTNVNDLIKNRDCVGYQGGAFVKDILLGLGFHEDQLKPFDSAKDADDLLSKGKSK-G 732

Query: 775 FDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADISRQVLNVTESEK 834
             AAFDE+ Y+K  LS+   KY M +P +K  GFGFAFP  SPL  + SR +LN+T++  
Sbjct: 733 IAAAFDEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNV 792

Query: 835 MNQIQKKWLGENCNSPSSGGNVGSSRLNLSSFWGLFLIAGSAAVVALLIYFAIFLYKEHH 894
             QI+ +W  +  + P     + S+RLNLSSF GLFLIAG+A   +LL++ A+FLY+  H
Sbjct: 793 TQQIEDRWFPKKNDCPDPMTALSSNRLNLSSFLGLFLIAGTAISFSLLVFVALFLYEHRH 852

Query: 895 TLRRTAADEGSNSPVLRKLRALLRTYDGRDLTSHTFRKSNNINVEAHAMDGASPSSNCPP 954
           TL      + S   + RKL+ L + +D +D+ SHTF+ S   N+       +SP ++  P
Sbjct: 853 TL-----GDDSEDSLWRKLKFLFKIFDEKDMNSHTFKNSAIHNI-------SSPMTHKTP 912

Query: 955 SPSNYSV--------QDASFEFFNGSGDSSPMNRNQTPPLHNQE 975
           SPS   +        Q+  FE    S   S       P +H+++
Sbjct: 913 SPSTVQITPWPQSPSQNREFELRRVSFSPSEERFTTQPIIHHED 929

BLAST of MC08g0462 vs. TAIR 10
Match: AT5G27100.1 (glutamate receptor 2.1 )

HSP 1 Score: 772.3 bits (1993), Expect = 4.7e-223
Identity = 416/883 (47.11%), Postives = 570/883 (64.55%), Query Frame = 0

Query: 39  MKPNRGAVRVLCFFAGCVVFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCI 98
           MK     V  L FF   +VFL+        AQN   +  VNVG+V D  +    M L CI
Sbjct: 1   MKRENNLVLSLLFFV--IVFLM----QVGEAQN--RITNVNVGIVNDIGTAYSNMTLLCI 60

Query: 99  DMSLSEFYAANPDYKTRIVLHTRNVGNDDVVGAAAAAVDLIKNKKVQAILGPTSSMQANF 158
           +MSLS+FY+++P+ +TR+V    +  N DVV AAAAA+DLI NK+V+AILGP +SMQA F
Sbjct: 61  NMSLSDFYSSHPETQTRLVTTVVDSKN-DVVTAAAAALDLITNKEVKAILGPWTSMQAQF 120

Query: 159 VIELGQKAHVPILSFTATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIY 218
           +IE+GQK+ VPI++++ATSP+LAS+RS YFFR   +DS QV AI  I+K FGWR+V P+Y
Sbjct: 121 MIEMGQKSQVPIVTYSATSPSLASIRSQYFFRATYDDSSQVHAIKEIIKLFGWREVAPVY 180

Query: 219 EDDEFGDGILPYLIDALQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLP 278
            DD FG+GI+P L D LQ++N R+ YR+VI+P AT D+I  EL ++ T+ +RVFVVH++ 
Sbjct: 181 VDDTFGEGIMPRLTDVLQEINVRIPYRTVISPNATDDEISVELLRMMTLPTRVFVVHLVE 240

Query: 279 SLAARLFAIADKIGMMSEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKLD 338
            LA+R FA A +IG+M +G+VWILT    ++L  M+ + + +M G LGVKT+VP+S +L+
Sbjct: 241 LLASRFFAKATEIGLMKQGYVWILTNTITDVLSIMNETEIETMQGVLGVKTYVPRSKELE 300

Query: 339 EFTVRWKRKFLSQNPTLNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK---NPNLT 398
            F  RW ++F      ++D  L+V+GLWA+DA  ALA+A+E  G +N T++K     N++
Sbjct: 301 NFRSRWTKRF-----PISD--LNVYGLWAYDATTALALAIEEAGTSNLTFVKMDAKRNVS 360

Query: 399 DLQSLGVSENGEKLRDFLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTP 458
           +LQ LGVS+ G KL   LS  +F+GLAGDF   NGELQ +  E+VNVNG  G  +GFW  
Sbjct: 361 ELQGLGVSQYGPKLLQTLSRVRFQGLAGDFQFINGELQPSVFEIVNVNGQGGRTIGFWMK 420

Query: 459 ENGLTKDWS---ASGT---------RSIIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGY 518
           E GL K+     AS T         R IIWPGDT + P+GWE+PT G RL+IGVP  + +
Sbjct: 421 EYGLFKNVDQKPASKTTFSSWQDRLRPIIWPGDTTSVPKGWEIPTNGKRLQIGVPVNNTF 480

Query: 519 SEFVRITK----NGTDAEGYCTDVFEAALAGLPYAVPFDYFPF-DGAYDELIMKVHDGFF 578
            +FV+ T+    N T   G+  D FEA +  +PY + +D+ PF DG YD L+ +V+ G +
Sbjct: 481 QQFVKATRDPITNSTIFSGFSIDYFEAVIQAIPYDISYDFIPFQDGGYDALVYQVYLGKY 540

Query: 579 DGAVGDITIVANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCF 638
           D  V D TI +NRS YVDF+LP+T SGV +VVP + + +  + +FL PLTL LW+ S   
Sbjct: 541 DAVVADTTISSNRSMYVDFSLPYTPSGVGLVVPVKDSVRRSSTIFLMPLTLALWLISLLS 600

Query: 639 FVFMGFVVWILEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFF 698
           F  +G VVW+LEHR+N DF GP  +Q+ T  WFSF  MVFA RE ++S  AR VV+IW+F
Sbjct: 601 FFIIGLVVWVLEHRVNPDFDGPGQYQLSTIFWFSFSIMVFAPRERVLSFWARVVVIIWYF 660

Query: 699 VVFILTQSYTASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGFK--N 758
           +V +LTQSYTASL SLLT Q L P++T+IN L+ +   VGYQ  SF+LG LR  GF   +
Sbjct: 661 LVLVLTQSYTASLASLLTTQHLHPTVTNINSLLAKGESVGYQ-SSFILGRLRDSGFSEAS 720

Query: 759 LRPYASPDELHKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAF 818
           L  Y SP+       +G + GG  A   E+PY+++FL ++ +KY M    +K DG GF F
Sbjct: 721 LVSYGSPEHCDALLSKGQAEGGVSAVLMEVPYVRIFLGQYCNKYKMVQTPFKVDGLGFVF 780

Query: 819 PMGSPLVADISRQVLNVTESEKMNQIQKKW---LGENC----NSPSSGGNVGSSRLNLSS 878
           P+GSPLVADISR +L V ES K NQ++  W   + E+C     +P    +V   +L   S
Sbjct: 781 PIGSPLVADISRAILKVEESNKANQLENAWFKPIDESCPDPLTNPDPNPSVSFRQLGFDS 840

Query: 879 FWGLFLIAGSAAVVALLIYFAIFLYKEHHTLRRTAADEGSNSP 893
           FW LFL+A     +ALL +   FL +  +        E  N P
Sbjct: 841 FWVLFLVAAIVCTMALLKFVYQFLKENPNQRNLRVLWEKFNEP 866

BLAST of MC08g0462 vs. TAIR 10
Match: AT2G24720.1 (glutamate receptor 2.2 )

HSP 1 Score: 754.2 bits (1946), Expect = 1.3e-217
Identity = 396/856 (46.26%), Postives = 566/856 (66.12%), Query Frame = 0

Query: 57  VFLLGTTALTAAAQNATAMAAVNVGVVLDFESRIGKMGLSCIDMSLSEFYAANPDYKTRI 116
           +FL     L ++         VN+GVV D  +    + + CI+MSL++FY++ P ++TR+
Sbjct: 11  LFLFFFFCLESSRGQDNGKTQVNIGVVSDVGTSYPDVAMLCINMSLADFYSSRPQFQTRL 70

Query: 117 VLHTRNVGN--DDVVGAAAAAVDLIKNKKVQAILGPTSSMQANFVIELGQKAHVPILSFT 176
           V+   NVG+  +DVVGAA AA+DLIKNK+V+AILGP +SMQA+F+IE+GQK+ VP++S++
Sbjct: 71  VV---NVGDSKNDVVGAATAAIDLIKNKQVKAILGPWTSMQAHFLIEIGQKSRVPVVSYS 130

Query: 177 ATSPALASLRSPYFFRLAQNDSVQVAAISAIVKTFGWRQVVPIYEDDEFGDGILPYLIDA 236
           ATSP+L SLRSPYFFR    DS QV AI AI+K FGWR+VVP+Y D+ FG+GI+P L D+
Sbjct: 131 ATSPSLTSLRSPYFFRATYEDSSQVHAIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDS 190

Query: 237 LQDVNARVTYRSVINPAATGDQIGEELYKLTTMQSRVFVVHMLPSLAARLFAIADKIGMM 296
           LQD+N R+ YRSVI   AT   I  EL K+  M +RVF+VHM  SLA+ +F  A ++G+M
Sbjct: 191 LQDINVRIPYRSVIPLNATDQDISVELLKMMNMPTRVFIVHMSSSLASTVFIKAKELGLM 250

Query: 297 SEGFVWILTGATANLLQSMDSSVLHSMDGALGVKTFVPKSMKLDEFTVRWKRKFLSQNPT 356
             G+VWILT    + L+S++ + + +M+G LG+KT++PKS  L+ F  RWKR+F    P 
Sbjct: 251 KPGYVWILTNGVMDGLRSINETGIEAMEGVLGIKTYIPKSKDLETFRSRWKRRF----PQ 310

Query: 357 LNDPQLDVFGLWAHDAAKALAMAVERIGPANFTYLK---NPNLTDLQSLGVSENGEKLRD 416
           +   +L+V+GLWA+DA  ALAMA+E  G  N T+       N+++L  LG+S+ G KL  
Sbjct: 311 M---ELNVYGLWAYDATTALAMAIEDAGINNMTFSNVDTGKNVSELDGLGLSQFGPKLLQ 370

Query: 417 FLSTTKFRGLAGDFGVANGELQSATLEVVNVNGNWGNRVGFWTPENGLTK----DWSASG 476
            +ST +F+GLAGDF   +G+LQ +  E+VN+ G     +GFWT  NGL K    +  + G
Sbjct: 371 TVSTVQFKGLAGDFHFVSGQLQPSVFEIVNMIGTGERSIGFWTEGNGLVKKLDQEPRSIG 430

Query: 477 TRS--------IIWPGDTAAQPRGWEMPTMGTRLKIGVPRKDGYSEFVRITK----NGTD 536
           T S        IIWPG+  + P+GWE+PT G +L+IGVP++ G+++ V++T+    N T 
Sbjct: 431 TLSTWPDHLKHIIWPGEAVSVPKGWEIPTNGKKLRIGVPKRIGFTDLVKVTRDPITNSTV 490

Query: 537 AEGYCTDVFEAALAGLPYAVPFDYFPFD-------GAYDELIMKVHDGFFDGAVGDITIV 596
            +G+C D FEA +  +PY V +++FPF+       G +++L+ +V+ G FD  VGD TI+
Sbjct: 491 VKGFCIDFFEAVIQAMPYDVSYEFFPFEKPNGEPAGNHNDLVHQVYLGQFDAVVGDTTIL 550

Query: 597 ANRSQYVDFTLPFTESGVSMVVPTQSNSKNRAWVFLKPLTLDLWITSFCFFVFMGFVVWI 656
           ANRS +VDFTLPF +SGV ++VP +   K   + FLKPL+++LW+T+  FF  +G  VW 
Sbjct: 551 ANRSSFVDFTLPFMKSGVGLIVPLKDEVKRDKFSFLKPLSIELWLTTLVFFFLVGISVWT 610

Query: 657 LEHRINEDFRGPPSHQIGTSLWFSFCTMVFAQRETLISNLARFVVVIWFFVVFILTQSYT 716
           LEHR+N DFRGP ++Q  T  WF+F TMVFA RE ++S  AR +VV W+FV+ +LTQSYT
Sbjct: 611 LEHRVNSDFRGPANYQASTIFWFAFSTMVFAPRERVLSFGARSLVVTWYFVLLVLTQSYT 670

Query: 717 ASLTSLLTVQQLQPSITDINELMKRQPWVGYQEGSFVLGLLRSVGF--KNLRPYASPDEL 776
           ASL SLLT QQL P+IT ++ L+ R   VGYQ  SF+LG L   GF   +L P+ + +E 
Sbjct: 671 ASLASLLTSQQLNPTITSMSSLLHRGETVGYQRTSFILGKLNETGFPQSSLVPFDTAEEC 730

Query: 777 HKFFKRGSSNGGFDAAFDEIPYIKLFLSKFPDKYTMSDPIYKADGFGFAFPMGSPLVADI 836
            +  K+G  NGG  AAF   PY++LFL ++ + Y M +  +  DGFGF FP+GSPLVAD+
Sbjct: 731 DELLKKGPKNGGVAAAFLGTPYVRLFLGQYCNTYKMVEEPFNVDGFGFVFPIGSPLVADV 790

Query: 837 SRQVLNVTESEKMNQIQKKWL---GENC----NSPSSGGNVGSSRLNLSSFWGLFLIAGS 876
           SR +L V ES K  +++  W     ++C     +P S   V + +L + SFW LFL+   
Sbjct: 791 SRAILKVAESPKAVELEHAWFKKKEQSCPDPVTNPDSNPTVTAIQLGVGSFWFLFLVVFV 850