MC08g0452 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0452
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	lariat debranching enzyme
Location	MC08: 3626036 .. 3629854 (+)
RNA-Seq Expression	MC08g0452
Synteny	MC08g0452

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATAAGTTAAACTTTAAATATTAATTATTTAAAATTATTTTTAATTATATAAAAAAATATTAAGTAAATATTAAAATAATTATATAAAAATATCTTTTACAAAATTAATTAGGGTTAAATCTCTTTCACTAAAATCAGTGGTTCTAGAATAATTTTGAAATAAGCTATATATTTGTAATATTTTGAACTCACTGTGACGTATGTGTGTTTTTAACTGTATTTTGCTGTTATTTTGGAACCTTTGTTGCTTGCCTTTGGAGAAGAAAAATAGAGGGTTTAAGGGGGCGTATGAAATGAAATGAACTGAAGCATTTCACAAAAAGTTGCTTTCAAATTCAAGAAATTTCCTCGGAACCGCCTCTCCCGCGAAGACGAAGAAGCAGCAGCAGCAGCATGAGGATTGCAGTGGAAGGTTGCATGCACGGCGACCTCGACAATGTCTACAAAACTCTCCAACACATAGAGAAAGTTCAAGGCATTAAAATCGACCTTCTTCTCTGCTGTGGCGACTTTCAGGTACTTCTTTCCTTCACTTGGACTTGCATCGCATTTCCCCTAATCTTTTCTATTTTCAAGTATCTGCACTCCCAAAATCATTTCAATTTTCTGCGCTATTTAGGCTGTTAGAAATGAAAACGATCTGAAGAGCTTAAATGTGCCCCCGAAATATCGGTCCATGAACTCCTTCTGGAAGTATTACTCTGGAGCTGAAGTCGCTCCCTATCCTACTATTTTCATTGGTGGAAATCACGAAGCGTCTAATTATCTGTGGGAATTGTGAGTTACTTGAGCTTAACTTGTGTTATATCTTAACGGGTCTCCATGAGTGCTTCGGTTGTTTGTGTTCTTGGTGTGTAGAGCTATGAAATTTTATTTTGGGGGTAAGAGAGATTCTGAAAAGTGAGTCACAGTTGTGGATCGCTTGCGTATAGCTTTTGATTTTTCGAATGCTGCGCTTAATTGTGAAAAATATCTCGATTGAATAATTGGCAATAGAATTATATGAGGTGCGTGTAAGCTGGTCTGGATACTCACGGATAGTATAAAAAAAAAGTAAAATTAATAATTTGCAATAAAGTTTCCCGACATGCTATTCAGATGTTCAGATACTTTGTCATTCTATTGAACAATTATGTGAGATCTTTGTAAACTAGTCTCTACGCCCTCAATTTTAATTGCTTCTCATGTTTTTTTTTTTTGTTTCCGTTTGATTGTTGTTTTTCTGAGGCTTTGTGTCCTTTTATGCACATGGCACATAAATTATCAGGATCCAATTGTTATTTTAGGTACTATGGAGGGTGGGCTGCGCCTAATATTTACTTCTTGGGCTTTGCTGGTGTAGTCAAGTTTGGAAATATCCGTATTGGTGGGCTGTCTGGGATTTATAAGGCACGCAATTATCATTTAGGTGGGTGAATAATTTTCCATCCGTTATTTATGTAGTCGTGAAACAGAGTGAGTGAGTTATCTGGATTGATTAACATGCAGTCGAGGTTTTCTTTTGTTCCTTTCTTGTGCTATATTTGGTCATGGAATCTGGTATATATTAATGATCTTGAATGAGGTAATAGTTCACAGAACGAGTAGTTGCATTTTCTACAGTAGGTTTCATTTTTTGGGTTGGGTTTTGTTTCTTGAGTGCTTTTGAAGAGACTCTTTCTCAATTTATTATCTGTTGAGGAACTTTTTTATTGGTAAAGCTTTTGGAAGTAGATCCATATGCAGGTAACATACTAGCTCTTCATTAAATTTTTAGAATATAGGAAGTCCTTCCTCCTGGGAGTAAGTTTGCATATTAAAAAAGGGTTGGTATTTTAAATGTATGGTTCCGTATCTTTATCTTTGTGCTGCCCTGTTATTTTTCCGCAATATGTGTTAAGCTGCATGCAGGATAATCTCCTTCACATGCAATGGGCAGGTCACCATGAGAGGCCTCCGTACAATGAGAATACTATAAGATCTGTTTATCACGTCCGTGAATATGATGTTCAGAAGCTTATGCAACTTGAGGAACCCATTGACATTTTTCTTTCCCATGATTGGCCAGTTGGCATCACTGATTATGGAGACTGGGAGCAACTTGTTAGGTCCAAACCCTTTTTTGAGAAGGAGGTAGCTAAAAATATTGTTACTACTTACTCAACAACTTTAAAAGTGCATCCATAGTAATTATCTGCATGATATGAACTGCTGAGACATAGATGTAATTTTTTTTTTTTTTTTAATTGGAGCTTAGATGTAAGGTTTATTTTACTATCATTTTGAGGGCATCCCACCTCTGACATGAACTTCTATGTTATCATTGTCAGATTGAATTGTACATAGTTGATGTGAAAAGTACCTGATCTAATGCTACGTCTACCTATCACTCTTAAATCACATTACCCTTCTAAGATTTTAGTAATTAACTTGATTAGCACTTAGCACAGGACAAAAGTGAAGGTCAGAAGAGGATTTTATTGCTATTAAATTCCATAGAAGTTGTTGAAATTGGTTAATTGATGGGACTGGGACCTGAATTATAACGACTAGAACTTCTTGTAAAGTCAGAGGCAAAGCATTTGGATCCAGAAAGCTAGTATAGATTATGATTCATGCATAGCTTCAGCTTTAAGTTATGAAGAAGGCACAAGTAAATTAAACATATTGTTTTAAAGAACCTTGAGGAATCTGACTTTTATCTAGAATGGTCCCCAGAGGAACTTAAGTAGACTCCAGAAAAAATGTTCCAACATCTTCTGATATGTTTACTTGCTGATTTTGACCTTTGTACCAGTAAAGTTTTCCTGATTATGCTGTTGAGGTTCATGACTTATGTAGTTGAATTTTTAACGCCAGATTCAGGAAAGAAGTCTTGGGAGTAAGGCTGCTGCCGAGTTGCTGGAAAAGTTGAGACCACCTTATTGGTTTTCAGCCCACCTGCACTGTAAGTTTGCTGCTCTTGTGCAACATGGTGAAGGTGGCCCTTTGACAAAATTTCTTGCGCTTGATAAGTGTCTCCCTAGGCACAAATTTTTACAGGTACTTCAATTTTGATTTGAACTCATTAATAGTTCTCCATAAGGTCCTTTTTTGGATTTTGCTAGATGCTGATGATTTCAAGCCTTTAACTGTGTGCTTGTGGTTTTTTAGGTTTTTGAGATTGAAACAGAACCTGGACCTTATGAGATTCATTATGATGAAGAATGGTTGGCAATAACACGAAGGTACAATGCAATCTTCCCATTGACGGCCAGAAATGCAAATTATGGGTGCGTAAGCATTTACAATAACTCTTTATGAAGATTTTATTGGACATTATGACCCCCCATTCTCATTTGTGGGCTATTGACAGTATGAGTTGTAACTCTCCATTTTGTAATATCAGAGGTGTACAACTTCAAATGCAAGATTGTCGACAATGGGTCCATAGCAAGTTGGAAGAAAGAGGAACAAAACCCTTTGAATTCGTTCAGACTGTTCCTTGTTATGATCCAACAAGATCTGTCTCCAGTAGTCCGCTTGCTGGTAATTCAAATAATTTTTTCAATCAAGTTATATCTGTGTAAGTATTCTGATCCAAAAAAAAAATATATCTGTGTAAGTAGAATGAACTTAGATCTTGTGTTCTTTTTCTAGTTCACTGTATTAGTACCAGGGAGGGTGGTTTTCAATTCTTAAGGTATTGATGGATAGACTGTATGACAGGATATCCCCGAAATCCTCAGACTGAGTCTTTGCTGAAGTTTCTCGAACTCCCTTATCTTCTTGATGACATGTCAGTACCTGCTGAACCATCTGGGAGTTCTATTCCTTTCTCAGATAGTGGTAAACTGTTT

mRNA sequence

GAATAAGTTAAACTTTAAATATTAATTATTTAAAATTATTTTTAATTATATAAAAAAATATTAAGTAAATATTAAAATAATTATATAAAAATATCTTTTACAAAATTAATTAGGGTTAAATCTCTTTCACTAAAATCAGTGGTTCTAGAATAATTTTGAAATAAGCTATATATTTGTAATATTTTGAACTCACTGTGACGTATGTGTGTTTTTAACTGTATTTTGCTGTTATTTTGGAACCTTTGTTGCTTGCCTTTGGAGAAGAAAAATAGAGGGTTTAAGGGGGCGTATGAAATGAAATGAACTGAAGCATTTCACAAAAAGTTGCTTTCAAATTCAAGAAATTTCCTCGGAACCGCCTCTCCCGCGAAGACGAAGAAGCAGCAGCAGCAGCATGAGGATTGCAGTGGAAGGTTGCATGCACGGCGACCTCGACAATGTCTACAAAACTCTCCAACACATAGAGAAAGTTCAAGGCATTAAAATCGACCTTCTTCTCTGCTGTGGCGACTTTCAGGCTGTTAGAAATGAAAACGATCTGAAGAGCTTAAATGTGCCCCCGAAATATCGGTCCATGAACTCCTTCTGGAAGTATTACTCTGGAGCTGAAGTCGCTCCCTATCCTACTATTTTCATTGGTGGAAATCACGAAGCGTCTAATTATCTGTGGGAATTGTACTATGGAGGGTGGGCTGCGCCTAATATTTACTTCTTGGGCTTTGCTGGTGTAGTCAAGTTTGGAAATATCCGTATTGGTGGGCTGTCTGGGATTTATAAGGCACGCAATTATCATTTAGGTCACCATGAGAGGCCTCCGTACAATGAGAATACTATAAGATCTGTTTATCACGTCCGTGAATATGATGTTCAGAAGCTTATGCAACTTGAGGAACCCATTGACATTTTTCTTTCCCATGATTGGCCAGTTGGCATCACTGATTATGGAGACTGGGAGCAACTTGTTAGGTCCAAACCCTTTTTTGAGAAGGAGATTCAGGAAAGAAGTCTTGGGAGTAAGGCTGCTGCCGAGTTGCTGGAAAAGTTGAGACCACCTTATTGGTTTTCAGCCCACCTGCACTGTAAGTTTGCTGCTCTTGTGCAACATGGTGAAGGTGGCCCTTTGACAAAATTTCTTGCGCTTGATAAGTGTCTCCCTAGGCACAAATTTTTACAGGTTTTTGAGATTGAAACAGAACCTGGACCTTATGAGATTCATTATGATGAAGAATGGTTGGCAATAACACGAAGGTACAATGCAATCTTCCCATTGACGGCCAGAAATGCAAATTATGGAGGTGTACAACTTCAAATGCAAGATTGTCGACAATGGGTCCATAGCAAGTTGGAAGAAAGAGGAACAAAACCCTTTGAATTCGTTCAGACTGTTCCTTGTTATGATCCAACAAGATCTGTCTCCAGTAGTCCGCTTGCTGGATATCCCCGAAATCCTCAGACTGAGTCTTTGCTGAAGTTTCTCGAACTCCCTTATCTTCTTGATGACATGTCAGTACCTGCTGAACCATCTGGGAGTTCTATTCCTTTCTCAGATAGTGGTAAACTGTTT

Coding sequence (CDS)

ATGAGGATTGCAGTGGAAGGTTGCATGCACGGCGACCTCGACAATGTCTACAAAACTCTCCAACACATAGAGAAAGTTCAAGGCATTAAAATCGACCTTCTTCTCTGCTGTGGCGACTTTCAGGCTGTTAGAAATGAAAACGATCTGAAGAGCTTAAATGTGCCCCCGAAATATCGGTCCATGAACTCCTTCTGGAAGTATTACTCTGGAGCTGAAGTCGCTCCCTATCCTACTATTTTCATTGGTGGAAATCACGAAGCGTCTAATTATCTGTGGGAATTGTACTATGGAGGGTGGGCTGCGCCTAATATTTACTTCTTGGGCTTTGCTGGTGTAGTCAAGTTTGGAAATATCCGTATTGGTGGGCTGTCTGGGATTTATAAGGCACGCAATTATCATTTAGGTCACCATGAGAGGCCTCCGTACAATGAGAATACTATAAGATCTGTTTATCACGTCCGTGAATATGATGTTCAGAAGCTTATGCAACTTGAGGAACCCATTGACATTTTTCTTTCCCATGATTGGCCAGTTGGCATCACTGATTATGGAGACTGGGAGCAACTTGTTAGGTCCAAACCCTTTTTTGAGAAGGAGATTCAGGAAAGAAGTCTTGGGAGTAAGGCTGCTGCCGAGTTGCTGGAAAAGTTGAGACCACCTTATTGGTTTTCAGCCCACCTGCACTGTAAGTTTGCTGCTCTTGTGCAACATGGTGAAGGTGGCCCTTTGACAAAATTTCTTGCGCTTGATAAGTGTCTCCCTAGGCACAAATTTTTACAGGTTTTTGAGATTGAAACAGAACCTGGACCTTATGAGATTCATTATGATGAAGAATGGTTGGCAATAACACGAAGGTACAATGCAATCTTCCCATTGACGGCCAGAAATGCAAATTATGGAGGTGTACAACTTCAAATGCAAGATTGTCGACAATGGGTCCATAGCAAGTTGGAAGAAAGAGGAACAAAACCCTTTGAATTCGTTCAGACTGTTCCTTGTTATGATCCAACAAGATCTGTCTCCAGTAGTCCGCTTGCTGGATATCCCCGAAATCCTCAGACTGAGTCTTTGCTGAAGTTTCTCGAACTCCCTTATCTTCTTGATGACATGTCAGTACCTGCTGAACCATCTGGGAGTTCTATTCCTTTCTCAGATAGTGGTAAACTGTTT

Protein sequence

MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRSMNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRIGGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGITDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEGGPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYGGVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKFLELPYLLDDMSVPAEPSGSSIPFSDSGKLF

Homology

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BLAST of MC08g0452 vs. ExPASy Swiss-Prot
Match: Q94K01 (Lariat debranching enzyme OS=Arabidopsis thaliana OX=3702 GN=DBR1 PE=2 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.1e-167
Identity = 278/396 (70.20%), Postives = 320/396 (80.81%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           M+IA+EGCMHGDLDNVYKT+QH E++   K+DLLLCCGDFQAVRNE D+ SLNVP KYR 
Sbjct: 1   MKIAIEGCMHGDLDNVYKTIQHYEQIHNTKVDLLLCCGDFQAVRNEKDMDSLNVPRKYRE 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           M SFWKYYSG EVAP PTIFIGGNHEASNYLWELYYGGWAA NIYFLGFAGVVKFGN+RI
Sbjct: 61  MKSFWKYYSGQEVAPIPTIFIGGNHEASNYLWELYYGGWAATNIYFLGFAGVVKFGNVRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY  R+Y  GH ERPPYNE+TIRSVYHVR+YDVQKLMQLEEP+DIFLSHDWPVGI
Sbjct: 121 GGLSGIYNERHYRSGHFERPPYNESTIRSVYHVRDYDVQKLMQLEEPLDIFLSHDWPVGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYGD E L+R KP+F +EI+E++LGSK AA LLEKL+P YWFSAHLHCKFAA VQHG  
Sbjct: 181 TDYGDSESLMRQKPYFRQEIEEKTLGSKPAALLLEKLKPQYWFSAHLHCKFAAAVQHGND 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           G +TKFLALDKCLP  KFLQ+ EIE+EPGP+E+ YDEEWLAITR++N+IFPLT R  N  
Sbjct: 241 GSVTKFLALDKCLPGKKFLQIIEIESEPGPFEVLYDEEWLAITRKFNSIFPLTRRYTNVS 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
                +Q+ R+WV  KLEER  KPFEF +TVP Y+P++ V  S +   P+NPQT SLL+ 
Sbjct: 301 TAG-TIQESREWVRKKLEERQFKPFEFARTVPAYNPSQRVFDS-IPEIPQNPQTLSLLEL 360

Query: 361 LELPYLLDD-------MSVPAEPSGSSIPFSDSGKL 390
           L LPYLLD          +PA  + S +P  DS ++
Sbjct: 361 LGLPYLLDSSPVTGERTDIPASLAPSDLPTYDSEEI 394

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BLAST of MC08g0452 vs. ExPASy Swiss-Prot
Match: Q6AU07 (Lariat debranching enzyme OS=Oryza sativa subsp. japonica OX=39947 GN=DBR1 PE=2 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 8.4e-165
Identity = 262/368 (71.20%), Postives = 306/368 (83.15%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           M+IAVEGCMHG+LD VY TL+ +EK +G+KIDLLLCCGDFQAVRNENDL+ LNV P++R 
Sbjct: 1   MKIAVEGCMHGELDKVYDTLRELEKAEGVKIDLLLCCGDFQAVRNENDLQCLNVKPRFRE 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           M SFWKYYSG  VAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI
Sbjct: 61  MKSFWKYYSGQAVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIYK ++YHLGH+ERPPYNENTIRSVYHVR YDV KLM ++EP+DIF+SHDWP+GI
Sbjct: 121 GGLSGIYKQQHYHLGHYERPPYNENTIRSVYHVRHYDVLKLMHVKEPLDIFMSHDWPLGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           T+YG+W++L+R K FFE+E+ +R+LGS+ AA LL KL+PPYWFSAHLHCKF A++QHGEG
Sbjct: 181 TEYGNWQKLIREKRFFEEEVNKRTLGSEPAARLLNKLKPPYWFSAHLHCKFPAVIQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GP TKFLALDKCLPR  FLQV +I + PGP+EI YDEEWLAITR++N +F LT       
Sbjct: 241 GPTTKFLALDKCLPRRGFLQVIDIPSGPGPHEIQYDEEWLAITRKFNNVFSLTRMPFTML 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
             Q+  QD  QWV +KL  RG KP +FVQT   YDP+   S+  +  + RNPQTES L+ 
Sbjct: 301 DEQVDTQDDLQWVRNKLNARGAKPIDFVQTAASYDPSCQASNPSITVHCRNPQTESFLQL 360

Query: 361 LELPYLLD 369
           L LPYLLD
Sbjct: 361 LNLPYLLD 368

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BLAST of MC08g0452 vs. ExPASy Swiss-Prot
Match: Q6P886 (Lariat debranching enzyme OS=Xenopus tropicalis OX=8364 GN=dbr1 PE=2 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 5.6e-108
Identity = 190/368 (51.63%), Postives = 255/368 (69.29%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           M+IAVEGC HG+LD +Y+T+Q +EK +  K+DLLLCCGDFQAVRNE D+K + VP KYR 
Sbjct: 1   MKIAVEGCCHGELDKIYETIQFLEKKENTKVDLLLCCGDFQAVRNEGDMKCMAVPQKYRQ 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           M +F+KYYSG ++AP  TIFIGGNHEASNYL EL YGGW APNIY++G+AGVVK+  +RI
Sbjct: 61  MQTFYKYYSGEKLAPILTIFIGGNHEASNYLQELPYGGWVAPNIYYVGYAGVVKYRGVRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GG+SGI+K+ +Y  GH ERPPY+++T+RS YHVR  +V +L QL+EP+DIF+SHDWP  I
Sbjct: 121 GGISGIFKSHDYRKGHFERPPYSKDTVRSAYHVRNIEVFRLKQLKEPMDIFMSHDWPRSI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQH--- 240
             YG+ +QL++ K FF +E++  +LGS AA+ELL  ++P YWFSAHLH KFAA +QH   
Sbjct: 181 YHYGNKKQLLKKKDFFRQEVENNTLGSPAASELLLHIQPSYWFSAHLHVKFAAFMQHQTN 240

Query: 241 --GEGGPLTKFLALDKCLPRHKFLQVFEIETEPG-PYEIHYDEEWLAITRRYNAIFPLTA 300
             GE    TKFLALDKCLP  +FLQ+ ++E +PG P  + YD EWLA+ +    +  +T+
Sbjct: 241 VEGEIPKATKFLALDKCLPHREFLQIVDVEHDPGKPDCLEYDLEWLAVLKATKDLLNITS 300

Query: 301 RNANY---GGVQLQ-----MQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLA 355
           +  N     G+  +      +  ++ +   L      P  F  T  CYDP  +     +A
Sbjct: 301 KTWNMPENNGLHSRWDFSASEKTKREILDDLGHDIKIPCNFCMTTACYDP-NNPQYKRVA 360

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BLAST of MC08g0452 vs. ExPASy Swiss-Prot
Match: Q6GPB8 (Lariat debranching enzyme A OS=Xenopus laevis OX=8355 GN=dbr1-a PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.6e-107
Identity = 191/367 (52.04%), Postives = 254/367 (69.21%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           M+IAVEGC HG+LD +Y+T+Q +EK +  K+DLLLCCGDFQAVRNE D+K + VP KYR 
Sbjct: 1   MKIAVEGCCHGELDKIYETIQFLEKKENTKVDLLLCCGDFQAVRNEGDMKCMAVPMKYRQ 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           M +F+KYYSG + AP  TIFIGGNHEASNYL EL YGGW APNIY++G+AGVVK+  +RI
Sbjct: 61  MQTFYKYYSGEKKAPILTIFIGGNHEASNYLQELPYGGWVAPNIYYMGYAGVVKYRGVRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GG+SGI+K+ +Y  GH ERPPY+++T+RS YHVR  +V KL QL+EP+DIFLSHDWP  I
Sbjct: 121 GGISGIFKSHDYRKGHFERPPYSKDTVRSAYHVRSIEVFKLKQLKEPMDIFLSHDWPRSI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQH--- 240
             YG+ +QL++ K FF +E+++ +LGS AA+ELL  ++P YWFSAHLH KFAA +QH   
Sbjct: 181 YHYGNKKQLLKKKDFFRQEVEDNTLGSPAASELLLHIQPSYWFSAHLHVKFAAFMQHQNN 240

Query: 241 -GEGGPLTKFLALDKCLPRHKFLQVFEIETEPG-PYEIHYDEEWLAITRRYNAIFPLTAR 300
            GE    TKFLALDKCLP  +FLQ+ ++E +P  P  + YD EWLA+ +    +  +T++
Sbjct: 241 VGEIPKATKFLALDKCLPHREFLQIVDMEHDPSKPECLEYDLEWLAVLKATKDLLNITSK 300

Query: 301 NANY---GGVQLQ-----MQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAG 355
             N     G+  +      ++ ++ V   L      P  F  T  CYDP  +     +  
Sbjct: 301 TWNMPENNGLHARWDFSMSEETKREVLDDLGHDIKIPCNFSVTTACYDP-NNPQYKRMPT 360

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BLAST of MC08g0452 vs. ExPASy Swiss-Prot
Match: Q9UK59 (Lariat debranching enzyme OS=Homo sapiens OX=9606 GN=DBR1 PE=1 SV=2)

HSP 1 Score: 389.4 bits (999), Expect = 4.7e-107
Identity = 197/372 (52.96%), Postives = 249/372 (66.94%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MR+AV GC HG+LD +Y+TL   E+     +DLLLCCGDFQAVRNE DL+ + VPPKYR 
Sbjct: 1   MRVAVAGCCHGELDKIYETLALAERRGPGPVDLLLCCGDFQAVRNEADLRCMAVPPKYRH 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           M +F++YYSG + AP  T+FIGGNHEASN+L EL YGGW APNIY+LG AGVVK+  +RI
Sbjct: 61  MQTFYRYYSGEKKAPVLTLFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GG+SGI+K+ +Y  GH E PPYN +TIRS+YHVR  +V KL QL++PIDIFLSHDWP  I
Sbjct: 121 GGISGIFKSHDYRKGHFECPPYNSSTIRSIYHVRNIEVYKLKQLKQPIDIFLSHDWPRSI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQH--- 240
             YG+ +QL+++K FF +E++  +LGS AA+ELLE L+P YWFSAHLH KFAAL+QH   
Sbjct: 181 YHYGNKKQLLKTKSFFRQEVENNTLGSPAASELLEHLKPTYWFSAHLHVKFAALMQHQAK 240

Query: 241 --GEGGPLTKFLALDKCLPRHKFLQVFEIETEP-GPYEIHYDEEWLAITRRYNAIFPLTA 300
             G+    TKFLALDKCLP   FLQ+ EIE +P  P  + YD EWL I R  + +  +T 
Sbjct: 241 DKGQTARATKFLALDKCLPHRDFLQILEIEHDPSAPDYLEYDIEWLTILRATDDLINVTG 300

Query: 301 RNAN------------YGGVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSS 355
           R  N            Y   +  M++    V  KL      P  F  T  CYDP++  + 
Sbjct: 301 RLWNMPENNGLHARWDYSATEEGMKE----VLEKLNHDLKVPCNFSVTAACYDPSKPQTQ 360

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BLAST of MC08g0452 vs. NCBI nr
Match: XP_022132215.1 (lariat debranching enzyme [Momordica charantia])

HSP 1 Score: 815 bits (2104), Expect = 1.01e-297
Identity = 386/387 (99.74%), Postives = 386/387 (99.74%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI
Sbjct: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG
Sbjct: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
           GVQLQMQDCRQW HSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF
Sbjct: 301 GVQLQMQDCRQWXHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDSG 387
           LELPYLLDDMSVPAEPSGSSIPFSDSG
Sbjct: 361 LELPYLLDDMSVPAEPSGSSIPFSDSG 387

[Close]

BLAST of MC08g0452 vs. NCBI nr
Match: XP_038886565.1 (lariat debranching enzyme isoform X2 [Benincasa hispida])

HSP 1 Score: 739 bits (1909), Expect = 1.84e-268
Identity = 343/390 (87.95%), Postives = 370/390 (94.87%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVY+TLQ++E+VQ IKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY AR+YHLGH+ERPPYNENTIRS+YHVREYDV+KLMQ+EEPIDIFLSHDWP+GI
Sbjct: 121 GGLSGIYNARHYHLGHYERPPYNENTIRSIYHVREYDVRKLMQVEEPIDIFLSHDWPLGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYGDW+QL+R KPFFEKEIQE+SLGSKAAA+LLEKLRPPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGDWKQLIRFKPFFEKEIQEKSLGSKAAAQLLEKLRPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPR KFLQV EIE EPGPYEIHYDEEWLAITRR+N IFPLTA++ANYG
Sbjct: 241 GPLTKFLALDKCLPRRKFLQVIEIEAEPGPYEIHYDEEWLAITRRFNEIFPLTAKSANYG 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
            ++L+M+DCRQWV + L+ERGTKPF+FVQTVPCYDP+RSVS+S LAGYPRNPQTESLLKF
Sbjct: 301 SIKLEMEDCRQWVKTSLKERGTKPFDFVQTVPCYDPSRSVSNSQLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDSGKLF 390
           LELPYLLDDM     PSGSS+P SDS K F
Sbjct: 361 LELPYLLDDMLESDGPSGSSVPLSDSAKTF 390

[Close]

BLAST of MC08g0452 vs. NCBI nr
Match: XP_038886564.1 (lariat debranching enzyme isoform X1 [Benincasa hispida])

HSP 1 Score: 740 bits (1911), Expect = 1.86e-268
Identity = 343/390 (87.95%), Postives = 370/390 (94.87%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVY+TLQ++E+VQ IKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY AR+YHLGH+ERPPYNENTIRS+YHVREYDV+KLMQ+EEPIDIFLSHDWP+GI
Sbjct: 121 GGLSGIYNARHYHLGHYERPPYNENTIRSIYHVREYDVRKLMQVEEPIDIFLSHDWPLGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYGDW+QL+R KPFFEKEIQE+SLGSKAAA+LLEKLRPPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGDWKQLIRFKPFFEKEIQEKSLGSKAAAQLLEKLRPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPR KFLQV EIE EPGPYEIHYDEEWLAITRR+N IFPLTA++ANYG
Sbjct: 241 GPLTKFLALDKCLPRRKFLQVIEIEAEPGPYEIHYDEEWLAITRRFNEIFPLTAKSANYG 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
            ++L+M+DCRQWV + L+ERGTKPF+FVQTVPCYDP+RSVS+S LAGYPRNPQTESLLKF
Sbjct: 301 SIKLEMEDCRQWVKTSLKERGTKPFDFVQTVPCYDPSRSVSNSQLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDSGKLF 390
           LELPYLLDDM     PSGSS+P SDSG  F
Sbjct: 361 LELPYLLDDMLESDGPSGSSVPLSDSGSFF 390

[Close]

BLAST of MC08g0452 vs. NCBI nr
Match: XP_008445281.1 (PREDICTED: lariat debranching enzyme isoform X1 [Cucumis melo])

HSP 1 Score: 740 bits (1910), Expect = 2.65e-268
Identity = 345/390 (88.46%), Postives = 371/390 (95.13%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVY+TLQ++E+VQ IKIDLLLCCGDFQAVRNENDLKSLNV PKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY AR+YHLGHHERPPYNENTIRS+YHVREYDVQKLMQ+EEPIDIFLSHDWP+GI
Sbjct: 121 GGLSGIYNARHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYG+W+QLVR KPFFEKEIQERSLGSKAAAELLEKL+PPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPR +FLQV EIE+EPGPYEIHYDEEWLAITRR+N IFPLTA NANY 
Sbjct: 241 GPLTKFLALDKCLPRRQFLQVIEIESEPGPYEIHYDEEWLAITRRFNEIFPLTAENANYE 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
            ++L+M+DCRQWV ++L+ERGTKPF+FVQTVPCYDP+RSVS+SPLAGYPRNPQTESLLKF
Sbjct: 301 NIKLEMEDCRQWVKTRLKERGTKPFDFVQTVPCYDPSRSVSNSPLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDSGKLF 390
           LELPYLL+DM+    PSGSSIP SDSG  F
Sbjct: 361 LELPYLLEDMTKSDGPSGSSIPSSDSGSFF 390

[Close]

BLAST of MC08g0452 vs. NCBI nr
Match: XP_008445283.1 (PREDICTED: lariat debranching enzyme isoform X2 [Cucumis melo])

HSP 1 Score: 735 bits (1897), Expect = 1.11e-266
Identity = 343/386 (88.86%), Postives = 369/386 (95.60%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVY+TLQ++E+VQ IKIDLLLCCGDFQAVRNENDLKSLNV PKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY AR+YHLGHHERPPYNENTIRS+YHVREYDVQKLMQ+EEPIDIFLSHDWP+GI
Sbjct: 121 GGLSGIYNARHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYG+W+QLVR KPFFEKEIQERSLGSKAAAELLEKL+PPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPR +FLQV EIE+EPGPYEIHYDEEWLAITRR+N IFPLTA NANY 
Sbjct: 241 GPLTKFLALDKCLPRRQFLQVIEIESEPGPYEIHYDEEWLAITRRFNEIFPLTAENANYE 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
            ++L+M+DCRQWV ++L+ERGTKPF+FVQTVPCYDP+RSVS+SPLAGYPRNPQTESLLKF
Sbjct: 301 NIKLEMEDCRQWVKTRLKERGTKPFDFVQTVPCYDPSRSVSNSPLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDS 386
           LELPYLL+DM+    PSGSSIP SDS
Sbjct: 361 LELPYLLEDMTKSDGPSGSSIPSSDS 386

[Close]

BLAST of MC08g0452 vs. ExPASy TrEMBL
Match: A0A6J1BRU1 (lariat debranching enzyme OS=Momordica charantia OX=3673 GN=LOC111005131 PE=3 SV=1)

HSP 1 Score: 815 bits (2104), Expect = 4.87e-298
Identity = 386/387 (99.74%), Postives = 386/387 (99.74%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI
Sbjct: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG
Sbjct: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
           GVQLQMQDCRQW HSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF
Sbjct: 301 GVQLQMQDCRQWXHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDSG 387
           LELPYLLDDMSVPAEPSGSSIPFSDSG
Sbjct: 361 LELPYLLDDMSVPAEPSGSSIPFSDSG 387

[Close]

BLAST of MC08g0452 vs. ExPASy TrEMBL
Match: A0A1S3BD21 (lariat debranching enzyme isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488361 PE=3 SV=1)

HSP 1 Score: 740 bits (1910), Expect = 1.28e-268
Identity = 345/390 (88.46%), Postives = 371/390 (95.13%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVY+TLQ++E+VQ IKIDLLLCCGDFQAVRNENDLKSLNV PKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY AR+YHLGHHERPPYNENTIRS+YHVREYDVQKLMQ+EEPIDIFLSHDWP+GI
Sbjct: 121 GGLSGIYNARHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYG+W+QLVR KPFFEKEIQERSLGSKAAAELLEKL+PPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPR +FLQV EIE+EPGPYEIHYDEEWLAITRR+N IFPLTA NANY 
Sbjct: 241 GPLTKFLALDKCLPRRQFLQVIEIESEPGPYEIHYDEEWLAITRRFNEIFPLTAENANYE 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
            ++L+M+DCRQWV ++L+ERGTKPF+FVQTVPCYDP+RSVS+SPLAGYPRNPQTESLLKF
Sbjct: 301 NIKLEMEDCRQWVKTRLKERGTKPFDFVQTVPCYDPSRSVSNSPLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDSGKLF 390
           LELPYLL+DM+    PSGSSIP SDSG  F
Sbjct: 361 LELPYLLEDMTKSDGPSGSSIPSSDSGSFF 390

[Close]

BLAST of MC08g0452 vs. ExPASy TrEMBL
Match: A0A1S3BBU7 (lariat debranching enzyme isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488361 PE=3 SV=1)

HSP 1 Score: 735 bits (1897), Expect = 5.37e-267
Identity = 343/386 (88.86%), Postives = 369/386 (95.60%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVY+TLQ++E+VQ IKIDLLLCCGDFQAVRNENDLKSLNV PKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY AR+YHLGHHERPPYNENTIRS+YHVREYDVQKLMQ+EEPIDIFLSHDWP+GI
Sbjct: 121 GGLSGIYNARHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYG+W+QLVR KPFFEKEIQERSLGSKAAAELLEKL+PPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPR +FLQV EIE+EPGPYEIHYDEEWLAITRR+N IFPLTA NANY 
Sbjct: 241 GPLTKFLALDKCLPRRQFLQVIEIESEPGPYEIHYDEEWLAITRRFNEIFPLTAENANYE 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
            ++L+M+DCRQWV ++L+ERGTKPF+FVQTVPCYDP+RSVS+SPLAGYPRNPQTESLLKF
Sbjct: 301 NIKLEMEDCRQWVKTRLKERGTKPFDFVQTVPCYDPSRSVSNSPLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDS 386
           LELPYLL+DM+    PSGSSIP SDS
Sbjct: 361 LELPYLLEDMTKSDGPSGSSIPSSDS 386

[Close]

BLAST of MC08g0452 vs. ExPASy TrEMBL
Match: A0A5A7VC81 (Lariat debranching enzyme isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001870 PE=3 SV=1)

HSP 1 Score: 735 bits (1898), Expect = 8.96e-267
Identity = 345/391 (88.24%), Postives = 371/391 (94.88%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVY+TLQ++E+VQ IKIDLLLCCGDFQAVRNENDLKSLNV PKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHL-GHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVG 180
           GGLSGIY AR+YHL GHHERPPYNENTIRS+YHVREYDVQKLMQ+EEPIDIFLSHDWP+G
Sbjct: 121 GGLSGIYNARHYHLAGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLG 180

Query: 181 ITDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGE 240
           ITDYG+W+QLVR KPFFEKEIQERSLGSKAAAELLEKL+PPYWFSAHLHCKFAALVQHGE
Sbjct: 181 ITDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGE 240

Query: 241 GGPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANY 300
           GGPLTKFLALDKCLPR +FLQV EIE+EPGPYEIHYDEEWLAITRR+N IFPLTA NANY
Sbjct: 241 GGPLTKFLALDKCLPRRQFLQVIEIESEPGPYEIHYDEEWLAITRRFNEIFPLTAENANY 300

Query: 301 GGVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLK 360
             ++L+M+DCRQWV ++L+ERGTKPF+FVQTVPCYDP+RSVS+SPLAGYPRNPQTESLLK
Sbjct: 301 ENIKLEMEDCRQWVKTRLKERGTKPFDFVQTVPCYDPSRSVSNSPLAGYPRNPQTESLLK 360

Query: 361 FLELPYLLDDMSVPAEPSGSSIPFSDSGKLF 390
           FLELPYLL+DM+    PSGSSIP SDSG  F
Sbjct: 361 FLELPYLLEDMTKSDGPSGSSIPSSDSGSFF 391

[Close]

BLAST of MC08g0452 vs. ExPASy TrEMBL
Match: A0A0A0LQG6 (DBR1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G382800 PE=3 SV=1)

HSP 1 Score: 733 bits (1891), Expect = 1.01e-265
Identity = 340/390 (87.18%), Postives = 367/390 (94.10%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           MRIAVEGCMHGDLDNVY+TLQ++E+ Q IKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQAQNIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY  R+YHLGHHERPPYNENTIRS+YHVREYDVQKLMQ+EEPIDIFLSHDWP+GI
Sbjct: 121 GGLSGIYNERHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYG+W+QLVR KPFFEKEIQE+SLGSKAAA LLEKL+PPYWFSAHLHCKFAALVQHGEG
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQEKSLGSKAAAVLLEKLKPPYWFSAHLHCKFAALVQHGEG 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           GPLTKFLALDKCLPR +FLQV EIE+EPGPYEIHYDEEWLAIT+R+N IFPLTA+NANYG
Sbjct: 241 GPLTKFLALDKCLPRRQFLQVIEIESEPGPYEIHYDEEWLAITQRFNEIFPLTAKNANYG 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
            ++L+M+DCRQWV S+L+ERGTKPF+F QTVPCYDP RS+S+S LAGYPRNPQTESLLKF
Sbjct: 301 NIKLEMEDCRQWVKSRLKERGTKPFDFAQTVPCYDPARSISNSTLAGYPRNPQTESLLKF 360

Query: 361 LELPYLLDDMSVPAEPSGSSIPFSDSGKLF 390
           LELPYLLDDM+    PSGSSIP S SG  F
Sbjct: 361 LELPYLLDDMTKSDGPSGSSIPSSVSGSFF 390

[Close]

BLAST of MC08g0452 vs. TAIR 10
Match: AT4G31770.1 (debranching enzyme 1 )

HSP 1 Score: 589.3 bits (1518), Expect = 2.2e-168
Identity = 278/396 (70.20%), Postives = 320/396 (80.81%), Query Frame = 0


Query: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60
           M+IA+EGCMHGDLDNVYKT+QH E++   K+DLLLCCGDFQAVRNE D+ SLNVP KYR 
Sbjct: 1   MKIAIEGCMHGDLDNVYKTIQHYEQIHNTKVDLLLCCGDFQAVRNEKDMDSLNVPRKYRE 60

Query: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120
           M SFWKYYSG EVAP PTIFIGGNHEASNYLWELYYGGWAA NIYFLGFAGVVKFGN+RI
Sbjct: 61  MKSFWKYYSGQEVAPIPTIFIGGNHEASNYLWELYYGGWAATNIYFLGFAGVVKFGNVRI 120

Query: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180
           GGLSGIY  R+Y  GH ERPPYNE+TIRSVYHVR+YDVQKLMQLEEP+DIFLSHDWPVGI
Sbjct: 121 GGLSGIYNERHYRSGHFERPPYNESTIRSVYHVRDYDVQKLMQLEEPLDIFLSHDWPVGI 180

Query: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240
           TDYGD E L+R KP+F +EI+E++LGSK AA LLEKL+P YWFSAHLHCKFAA VQHG  
Sbjct: 181 TDYGDSESLMRQKPYFRQEIEEKTLGSKPAALLLEKLKPQYWFSAHLHCKFAAAVQHGND 240

Query: 241 GPLTKFLALDKCLPRHKFLQVFEIETEPGPYEIHYDEEWLAITRRYNAIFPLTARNANYG 300
           G +TKFLALDKCLP  KFLQ+ EIE+EPGP+E+ YDEEWLAITR++N+IFPLT R  N  
Sbjct: 241 GSVTKFLALDKCLPGKKFLQIIEIESEPGPFEVLYDEEWLAITRKFNSIFPLTRRYTNVS 300

Query: 301 GVQLQMQDCRQWVHSKLEERGTKPFEFVQTVPCYDPTRSVSSSPLAGYPRNPQTESLLKF 360
                +Q+ R+WV  KLEER  KPFEF +TVP Y+P++ V  S +   P+NPQT SLL+ 
Sbjct: 301 TAG-TIQESREWVRKKLEERQFKPFEFARTVPAYNPSQRVFDS-IPEIPQNPQTLSLLEL 360

Query: 361 LELPYLLDD-------MSVPAEPSGSSIPFSDSGKL 390
           L LPYLLD          +PA  + S +P  DS ++
Sbjct: 361 LGLPYLLDSSPVTGERTDIPASLAPSDLPTYDSEEI 394

The following BLAST results are available for this feature:

BLAST of MC08g0452 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94K01	3.1e-167	70.20	Lariat debranching enzyme OS=Arabidopsis thaliana OX=3702 GN=DBR1 PE=2 SV=1	[more]
Q6AU07	8.4e-165	71.20	Lariat debranching enzyme OS=Oryza sativa subsp. japonica OX=39947 GN=DBR1 PE=2 ...	[more]
Q6P886	5.6e-108	51.63	Lariat debranching enzyme OS=Xenopus tropicalis OX=8364 GN=dbr1 PE=2 SV=1	[more]
Q6GPB8	1.6e-107	52.04	Lariat debranching enzyme A OS=Xenopus laevis OX=8355 GN=dbr1-a PE=2 SV=1	[more]
Q9UK59	4.7e-107	52.96	Lariat debranching enzyme OS=Homo sapiens OX=9606 GN=DBR1 PE=1 SV=2	[more]

BLAST of MC08g0452 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132215.1	1.01e-297	99.74	lariat debranching enzyme [Momordica charantia]	[more]
XP_038886565.1	1.84e-268	87.95	lariat debranching enzyme isoform X2 [Benincasa hispida]	[more]
XP_038886564.1	1.86e-268	87.95	lariat debranching enzyme isoform X1 [Benincasa hispida]	[more]
XP_008445281.1	2.65e-268	88.46	PREDICTED: lariat debranching enzyme isoform X1 [Cucumis melo]	[more]
XP_008445283.1	1.11e-266	88.86	PREDICTED: lariat debranching enzyme isoform X2 [Cucumis melo]	[more]

BLAST of MC08g0452 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BRU1	4.87e-298	99.74	lariat debranching enzyme OS=Momordica charantia OX=3673 GN=LOC111005131 PE=3 SV...	[more]
A0A1S3BD21	1.28e-268	88.46	lariat debranching enzyme isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488361 PE=...	[more]
A0A1S3BBU7	5.37e-267	88.86	lariat debranching enzyme isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488361 PE=...	[more]
A0A5A7VC81	8.96e-267	88.24	Lariat debranching enzyme isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A0A0LQG6	1.01e-265	87.18	DBR1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G382800 PE=3 S...	[more]

BLAST of MC08g0452 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31770.1	2.2e-168	70.20	debranching enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007708	Lariat debranching enzyme, C-terminal	SMART	SM01124	DBR1_2	coord: 235..367 e-value: 1.2E-31 score: 121.2
IPR007708	Lariat debranching enzyme, C-terminal	PFAM	PF05011	DBR1	coord: 244..364 e-value: 9.0E-27 score: 94.2
IPR029052	Metallo-dependent phosphatase-like	GENE3D	3.60.21.10		coord: 8..244 e-value: 5.9E-7 score: 31.2
IPR029052	Metallo-dependent phosphatase-like	SUPERFAMILY	56300	Metallo-dependent phosphatases	coord: 1..245
IPR004843	Calcineurin-like phosphoesterase domain, ApaH type	PFAM	PF00149	Metallophos	coord: 1..229 e-value: 3.3E-14 score: 53.9
None	No IPR available	PANTHER	PTHR12849	RNA LARIAT DEBRANCHING ENZYME	coord: 1..377
IPR041816	Lariat debranching enzyme, N-terminal metallophosphatase domain	CDD	cd00844	MPP_Dbr1_N	coord: 3..259 e-value: 6.1319E-174 score: 483.677

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0452.1	MC08g0452.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0000398	mRNA splicing, via spliceosome
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0006397	mRNA processing
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008419	RNA lariat debranching enzyme activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds