MC08g0409 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0409
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionATP synthase subunit b
LocationMC08: 3300899 .. 3301540 (+)
RNA-Seq ExpressionMC08g0409
SyntenyMC08g0409
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAACGTGATCATGGCCTCTTCGAAGCCCCTCTCCCCACCTTCCATCTCCACCAAGCCAAAATTCGCTCTCCCCCTCCCCAAAACCCTAAACCAATTACTCCTTAGAAAGCCCCAATTCGCGGCCCTCTCATCCTCCACTCTCAAATCCCTCTCCCTCTTCGCCGCCACATCGCTCTCCTTCGCGCCCCCTTCCCTCGCCGAGGAAATCGAGAAGGCCGCCCTGTTCGACTTCAACCTCACTCTCCCCATCATGATGGCCCAGTTCCTGCTCCTCATGGTCGCCCTCGACAAGATCTACTTCTCCCCTCTCGGCAAGTTCATGGACGACAGAGACGCCGCCATCAAGGAGAAGCTCAACAGCGTCAAGGACACCTCCGGGGAGGTCAAGCAGCTGGACGAGCAGGCCGCCGCCGTCATGAGGGCAGCCAGGGCAGAGATTTCCGCCGCCCTTACTAAGATGAAGAAGGAAACTCAGGCGGAGGTGGAGCAGAAGCTTGCGGAGGGCAGGAAGAGAATTGATGCGGAGTTGCAGGAGGCGTTGGCCAATTTGGAGAATCAGAAGGAGGAGACCATTAAATCCCTCGACGCCCAAATCGCCGCTCTCAGCGACGAGATCGTCAAGAAGGTCCTTCCCGTT

mRNA sequence

ATGGCGAACGTGATCATGGCCTCTTCGAAGCCCCTCTCCCCACCTTCCATCTCCACCAAGCCAAAATTCGCTCTCCCCCTCCCCAAAACCCTAAACCAATTACTCCTTAGAAAGCCCCAATTCGCGGCCCTCTCATCCTCCACTCTCAAATCCCTCTCCCTCTTCGCCGCCACATCGCTCTCCTTCGCGCCCCCTTCCCTCGCCGAGGAAATCGAGAAGGCCGCCCTGTTCGACTTCAACCTCACTCTCCCCATCATGATGGCCCAGTTCCTGCTCCTCATGGTCGCCCTCGACAAGATCTACTTCTCCCCTCTCGGCAAGTTCATGGACGACAGAGACGCCGCCATCAAGGAGAAGCTCAACAGCGTCAAGGACACCTCCGGGGAGGTCAAGCAGCTGGACGAGCAGGCCGCCGCCGTCATGAGGGCAGCCAGGGCAGAGATTTCCGCCGCCCTTACTAAGATGAAGAAGGAAACTCAGGCGGAGGTGGAGCAGAAGCTTGCGGAGGGCAGGAAGAGAATTGATGCGGAGTTGCAGGAGGCGTTGGCCAATTTGGAGAATCAGAAGGAGGAGACCATTAAATCCCTCGACGCCCAAATCGCCGCTCTCAGCGACGAGATCGTCAAGAAGGTCCTTCCCGTT

Coding sequence (CDS)

ATGGCGAACGTGATCATGGCCTCTTCGAAGCCCCTCTCCCCACCTTCCATCTCCACCAAGCCAAAATTCGCTCTCCCCCTCCCCAAAACCCTAAACCAATTACTCCTTAGAAAGCCCCAATTCGCGGCCCTCTCATCCTCCACTCTCAAATCCCTCTCCCTCTTCGCCGCCACATCGCTCTCCTTCGCGCCCCCTTCCCTCGCCGAGGAAATCGAGAAGGCCGCCCTGTTCGACTTCAACCTCACTCTCCCCATCATGATGGCCCAGTTCCTGCTCCTCATGGTCGCCCTCGACAAGATCTACTTCTCCCCTCTCGGCAAGTTCATGGACGACAGAGACGCCGCCATCAAGGAGAAGCTCAACAGCGTCAAGGACACCTCCGGGGAGGTCAAGCAGCTGGACGAGCAGGCCGCCGCCGTCATGAGGGCAGCCAGGGCAGAGATTTCCGCCGCCCTTACTAAGATGAAGAAGGAAACTCAGGCGGAGGTGGAGCAGAAGCTTGCGGAGGGCAGGAAGAGAATTGATGCGGAGTTGCAGGAGGCGTTGGCCAATTTGGAGAATCAGAAGGAGGAGACCATTAAATCCCTCGACGCCCAAATCGCCGCTCTCAGCGACGAGATCGTCAAGAAGGTCCTTCCCGTT

Protein sequence

MANVIMASSKPLSPPSISTKPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAATSLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV
Homology
BLAST of MC08g0409 vs. ExPASy Swiss-Prot
Match: P31853 (ATP synthase subunit b', chloroplastic OS=Spinacia oleracea OX=3562 GN=ATPF2 PE=1 SV=2)

HSP 1 Score: 227.6 bits (579), Expect = 1.3e-58
Identity = 145/221 (65.61%), Postives = 171/221 (77.38%), Query Frame = 0

Query: 1   MANVIMASSKPLSPPSIST----KPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFA 60
           MAN+++ASS    P + +T    KPKF L     L     + P    L+ S LKS ++ A
Sbjct: 1   MANMLVASSSKTLPTTTTTTITPKPKFPLLKTPLLKLSPPQLPPLKHLNLSVLKSAAITA 60

Query: 61  A-TSLSF--APPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRD 120
              +LSF    PSLAEEIEKA+LFDFNLTLPI+MA+FL LM ALDKIY++PLG FMD RD
Sbjct: 61  TPLTLSFLLPYPSLAEEIEKASLFDFNLTLPIIMAEFLFLMFALDKIYYTPLGDFMDKRD 120

Query: 121 AAIKEKLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKR 180
           A+IKE+L+ VKDTS EVKQL+EQA AVMRAARAEISAAL KMKKETQ EVE KLAEGRK+
Sbjct: 121 ASIKEQLSGVKDTSSEVKQLEEQANAVMRAARAEISAALNKMKKETQLEVEAKLAEGRKK 180

Query: 181 IDAELQEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 215
           I+ ELQEAL +LE QKE+TIKSLD+QI+ALSD+IVKKVLPV
Sbjct: 181 IEVELQEALGSLEQQKEDTIKSLDSQISALSDDIVKKVLPV 221

BLAST of MC08g0409 vs. ExPASy Swiss-Prot
Match: A8J785 (ATP synthase subunit b', chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=ATPF2 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 6.5e-26
Identity = 76/196 (38.78%), Postives = 124/196 (63.27%), Query Frame = 0

Query: 18  STKPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAATSLSFAPPSLAEEIEKAALF 77
           + KP  A PL + + +   +KPQ   L+     +LS   A +L   P +     E   +F
Sbjct: 15  AAKPAAARPL-RLVVRASAQKPQ-QQLAQLAKPALSAIVANALMAMPAA----AEAGKIF 74

Query: 78  DFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKLNSVKDTSGEVKQLDEQA 137
           DFNLTLP+M  +FLLLMV L+K +F+P+GK +D+RD  I+ KL SVKD +G+V +L  +A
Sbjct: 75  DFNLTLPVMAGEFLLLMVFLEKTWFTPVGKVLDERDNLIRSKLGSVKDNTGDVDKLVLEA 134

Query: 138 AAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQEALANLENQKEETIKSLD 197
             ++++AR+++SA +   K   Q+E+++   E + +I AE++ ++A LE +    +KSLD
Sbjct: 135 ETILKSARSDVSAMINTKKAAKQSELDKTYNEAKAKITAEVESSIAGLEQESASMLKSLD 194

Query: 198 AQIAALSDEIVKKVLP 214
           AQ+  +S E++K+VLP
Sbjct: 195 AQVDKISAEVLKRVLP 204

BLAST of MC08g0409 vs. ExPASy Swiss-Prot
Match: P48085 (ATP synthase B' chain, cyanelle OS=Cyanophora paradoxa OX=2762 GN=atpF2 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.0e-15
Identity = 55/152 (36.18%), Postives = 86/152 (56.58%), Query Frame = 0

Query: 67  LAEEIEKA----ALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKLNS 126
           LA E E A     LFDF+ TLP+MM Q L+LM+ L+ +++ PL K +D+R   I+   N 
Sbjct: 8   LAIETEAAKPEGGLFDFDATLPVMMVQLLVLMLILNAVFYKPLIKILDERKEYIQSNFNE 67

Query: 127 VKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQEAL 186
            +    +  +L  Q    +  AR   S      + E Q  V +KL E +K+ D+EL  A 
Sbjct: 68  AEKCLAQAAELTTQYETKITDARQNASKLTNTTRSEIQRFVSEKLEEAQKKADSELASAT 127

Query: 187 ANLENQKEETIKSLDAQIAALSDEIVKKVLPV 215
             LE QK+E +KSL++++  LS +I++K+L +
Sbjct: 128 NKLELQKDEALKSLESEVQTLSTKILEKLLGI 159

BLAST of MC08g0409 vs. ExPASy Swiss-Prot
Match: Q05367 (ATP synthase subunit b' OS=Synechococcus sp. (strain PCC 6716) OX=32048 GN=atpF2 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.6e-14
Identity = 47/148 (31.76%), Postives = 87/148 (58.78%), Query Frame = 0

Query: 65  PSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKLNSVK 124
           P L   I +  +FDF+ TLP+M  QFL+L V L+ + + PLG+ +D+RD  I+  L   K
Sbjct: 11  PLLFRPILERLMFDFDATLPLMAVQFLILTVILNALLYKPLGQALDNRDEYIRTNLQQAK 70

Query: 125 DTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQEALAN 184
           +   +  +L  Q    +   R E  A + + + E Q     ++A  ++ + AEL +A A 
Sbjct: 71  ERLQQATELANQYEQELAYTRREAQAIIEEARAEAQKIATAEIAAAQQALQAELMKAQAE 130

Query: 185 LENQKEETIKSLDAQIAALSDEIVKKVL 213
           ++ QK+ T+++L+ Q+++LS++++ K+L
Sbjct: 131 IDQQKQATLQALEGQVSSLSEQLLAKLL 158

BLAST of MC08g0409 vs. ExPASy Swiss-Prot
Match: Q112Z3 (ATP synthase subunit b' OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=atpF2 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 5.7e-14
Identity = 51/147 (34.69%), Postives = 79/147 (53.74%), Query Frame = 0

Query: 66  SLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKLNSVKD 125
           ++ E  EK  LFD N TLP+M  QFLLL   LDKI++ PLGK +D R   I+E     K+
Sbjct: 9   AVEEVAEKGGLFDINATLPLMAIQFLLLAFVLDKIFYKPLGKAIDSRADYIRENQVKAKE 68

Query: 126 TSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQEALANL 185
              + KQL EQ        R +    +   + E +     K+A  +K    + ++A   +
Sbjct: 69  RLAKAKQLAEQYEQEFAQTRQKSQVVIVAAQAEAEKIAATKVAVAQKEAQVKREQAAQEI 128

Query: 186 ENQKEETIKSLDAQIAALSDEIVKKVL 213
           E QKE  ++ L+ Q+ +LS +I++K+L
Sbjct: 129 EKQKEVALEQLEEQVDSLSRQILEKLL 155

BLAST of MC08g0409 vs. NCBI nr
Match: XP_022131427.1 (ATP synthase subunit b', chloroplastic [Momordica charantia])

HSP 1 Score: 374 bits (960), Expect = 8.74e-130
Identity = 214/214 (100.00%), Postives = 214/214 (100.00%), Query Frame = 0

Query: 1   MANVIMASSKPLSPPSISTKPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAATSL 60
           MANVIMASSKPLSPPSISTKPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAATSL
Sbjct: 1   MANVIMASSKPLSPPSISTKPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAATSL 60

Query: 61  SFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKL 120
           SFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKL
Sbjct: 61  SFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKL 120

Query: 121 NSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQE 180
           NSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQE
Sbjct: 121 NSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQE 180

Query: 181 ALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           ALANLENQKEETIKSLDAQIAALSDEIVKKVLPV
Sbjct: 181 ALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214

BLAST of MC08g0409 vs. NCBI nr
Match: XP_038886736.1 (ATP synthase subunit b', chloroplastic [Benincasa hispida])

HSP 1 Score: 323 bits (828), Expect = 1.21e-109
Identity = 193/216 (89.35%), Postives = 201/216 (93.06%), Query Frame = 0

Query: 1   MANVIMASSKPLSPP-SISTK-PKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAAT 60
           MANVIMASSKPLSPP SIS K PKF++P    L    L+KPQ  +LSSSTLKSLSLFAAT
Sbjct: 1   MANVIMASSKPLSPPLSISNKTPKFSIPKTLALIPSFLKKPQSISLSSSTLKSLSLFAAT 60

Query: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKE 120
           SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMD+RD+AIKE
Sbjct: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDERDSAIKE 120

Query: 121 KLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAEL 180
           KLNSVKDTS EVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVE+KLAEGRK+IDAEL
Sbjct: 121 KLNSVKDTSEEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEEKLAEGRKKIDAEL 180

Query: 181 QEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           QEALANLENQK ETIKSLDAQIAALSDEIVKKVLPV
Sbjct: 181 QEALANLENQKVETIKSLDAQIAALSDEIVKKVLPV 216

BLAST of MC08g0409 vs. NCBI nr
Match: XP_008445233.1 (PREDICTED: ATP synthase subunit b', chloroplastic [Cucumis melo])

HSP 1 Score: 317 bits (811), Expect = 4.67e-107
Identity = 190/216 (87.96%), Postives = 198/216 (91.67%), Query Frame = 0

Query: 1   MANVIMASSKPLSPP-SISTK-PKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAAT 60
           MANVIMASSKPLSPP SIS K PKF++P   TL    L+ PQ  +LS+STLKSLSLFAAT
Sbjct: 1   MANVIMASSKPLSPPLSISNKTPKFSIPKTLTLIPQFLKNPQSISLSTSTLKSLSLFAAT 60

Query: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKE 120
           SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMV LDKIYFSPLGKFMD+RDAAIKE
Sbjct: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVGLDKIYFSPLGKFMDERDAAIKE 120

Query: 121 KLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAEL 180
           KL+SVKDTS  VKQLDEQAAAVMRAARAEISAAL KMKKETQAEVE+KLAEGRKRIDAEL
Sbjct: 121 KLSSVKDTSEGVKQLDEQAAAVMRAARAEISAALNKMKKETQAEVEEKLAEGRKRIDAEL 180

Query: 181 QEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           QEALANLENQK ETIKSLDAQIAALSDEIVKKVLPV
Sbjct: 181 QEALANLENQKAETIKSLDAQIAALSDEIVKKVLPV 216

BLAST of MC08g0409 vs. NCBI nr
Match: XP_004138722.1 (ATP synthase subunit b', chloroplastic [Cucumis sativus] >KGN62960.1 hypothetical protein Csa_022265 [Cucumis sativus])

HSP 1 Score: 316 bits (810), Expect = 6.63e-107
Identity = 188/216 (87.04%), Postives = 198/216 (91.67%), Query Frame = 0

Query: 1   MANVIMASSKPLSPP-SISTK-PKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAAT 60
           MANVIMASSKP SPP SIS K PKF++P   TL    L+KPQ  +LS+STLKSLSLFAAT
Sbjct: 1   MANVIMASSKPPSPPLSISNKTPKFSIPKTLTLIPQFLKKPQSISLSTSTLKSLSLFAAT 60

Query: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKE 120
           SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMV LDK+YFSPLGKFMD+RD AIKE
Sbjct: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVGLDKLYFSPLGKFMDERDTAIKE 120

Query: 121 KLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAEL 180
           KLNSVKDTS EVKQLD+QAAAVMRAARAEISAALTKMKKETQAEVE+KLAEGRKRID EL
Sbjct: 121 KLNSVKDTSEEVKQLDDQAAAVMRAARAEISAALTKMKKETQAEVEEKLAEGRKRIDVEL 180

Query: 181 QEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           QEALANLENQK ETIKSLDAQIAALSD+IVKKVLPV
Sbjct: 181 QEALANLENQKAETIKSLDAQIAALSDDIVKKVLPV 216

BLAST of MC08g0409 vs. NCBI nr
Match: KAA0064916.1 (ATP synthase subunit b' [Cucumis melo var. makuwa])

HSP 1 Score: 317 bits (811), Expect = 2.36e-106
Identity = 190/216 (87.96%), Postives = 198/216 (91.67%), Query Frame = 0

Query: 1   MANVIMASSKPLSPP-SISTK-PKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAAT 60
           MANVIMASSKPLSPP SIS K PKF++P   TL    L+ PQ  +LS+STLKSLSLFAAT
Sbjct: 48  MANVIMASSKPLSPPLSISNKTPKFSIPKTLTLIPQFLKNPQSISLSTSTLKSLSLFAAT 107

Query: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKE 120
           SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMV LDKIYFSPLGKFMD+RDAAIKE
Sbjct: 108 SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVGLDKIYFSPLGKFMDERDAAIKE 167

Query: 121 KLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAEL 180
           KL+SVKDTS  VKQLDEQAAAVMRAARAEISAAL KMKKETQAEVE+KLAEGRKRIDAEL
Sbjct: 168 KLSSVKDTSEGVKQLDEQAAAVMRAARAEISAALNKMKKETQAEVEEKLAEGRKRIDAEL 227

Query: 181 QEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           QEALANLENQK ETIKSLDAQIAALSDEIVKKVLPV
Sbjct: 228 QEALANLENQKAETIKSLDAQIAALSDEIVKKVLPV 263

BLAST of MC08g0409 vs. ExPASy TrEMBL
Match: A0A6J1BQZ6 (ATP synthase subunit b', chloroplastic OS=Momordica charantia OX=3673 GN=LOC111004643 PE=3 SV=1)

HSP 1 Score: 374 bits (960), Expect = 4.23e-130
Identity = 214/214 (100.00%), Postives = 214/214 (100.00%), Query Frame = 0

Query: 1   MANVIMASSKPLSPPSISTKPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAATSL 60
           MANVIMASSKPLSPPSISTKPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAATSL
Sbjct: 1   MANVIMASSKPLSPPSISTKPKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAATSL 60

Query: 61  SFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKL 120
           SFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKL
Sbjct: 61  SFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKEKL 120

Query: 121 NSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQE 180
           NSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQE
Sbjct: 121 NSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAELQE 180

Query: 181 ALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           ALANLENQKEETIKSLDAQIAALSDEIVKKVLPV
Sbjct: 181 ALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214

BLAST of MC08g0409 vs. ExPASy TrEMBL
Match: A0A1S3BD20 (ATP synthase subunit b', chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488320 PE=3 SV=1)

HSP 1 Score: 317 bits (811), Expect = 2.26e-107
Identity = 190/216 (87.96%), Postives = 198/216 (91.67%), Query Frame = 0

Query: 1   MANVIMASSKPLSPP-SISTK-PKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAAT 60
           MANVIMASSKPLSPP SIS K PKF++P   TL    L+ PQ  +LS+STLKSLSLFAAT
Sbjct: 1   MANVIMASSKPLSPPLSISNKTPKFSIPKTLTLIPQFLKNPQSISLSTSTLKSLSLFAAT 60

Query: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKE 120
           SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMV LDKIYFSPLGKFMD+RDAAIKE
Sbjct: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVGLDKIYFSPLGKFMDERDAAIKE 120

Query: 121 KLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAEL 180
           KL+SVKDTS  VKQLDEQAAAVMRAARAEISAAL KMKKETQAEVE+KLAEGRKRIDAEL
Sbjct: 121 KLSSVKDTSEGVKQLDEQAAAVMRAARAEISAALNKMKKETQAEVEEKLAEGRKRIDAEL 180

Query: 181 QEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           QEALANLENQK ETIKSLDAQIAALSDEIVKKVLPV
Sbjct: 181 QEALANLENQKAETIKSLDAQIAALSDEIVKKVLPV 216

BLAST of MC08g0409 vs. ExPASy TrEMBL
Match: A0A0A0LM10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G381850 PE=3 SV=1)

HSP 1 Score: 316 bits (810), Expect = 3.21e-107
Identity = 188/216 (87.04%), Postives = 198/216 (91.67%), Query Frame = 0

Query: 1   MANVIMASSKPLSPP-SISTK-PKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAAT 60
           MANVIMASSKP SPP SIS K PKF++P   TL    L+KPQ  +LS+STLKSLSLFAAT
Sbjct: 1   MANVIMASSKPPSPPLSISNKTPKFSIPKTLTLIPQFLKKPQSISLSTSTLKSLSLFAAT 60

Query: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKE 120
           SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMV LDK+YFSPLGKFMD+RD AIKE
Sbjct: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVGLDKLYFSPLGKFMDERDTAIKE 120

Query: 121 KLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAEL 180
           KLNSVKDTS EVKQLD+QAAAVMRAARAEISAALTKMKKETQAEVE+KLAEGRKRID EL
Sbjct: 121 KLNSVKDTSEEVKQLDDQAAAVMRAARAEISAALTKMKKETQAEVEEKLAEGRKRIDVEL 180

Query: 181 QEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           QEALANLENQK ETIKSLDAQIAALSD+IVKKVLPV
Sbjct: 181 QEALANLENQKAETIKSLDAQIAALSDDIVKKVLPV 216

BLAST of MC08g0409 vs. ExPASy TrEMBL
Match: A0A5A7VF07 (ATP synthase subunit b OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002340 PE=3 SV=1)

HSP 1 Score: 317 bits (811), Expect = 1.14e-106
Identity = 190/216 (87.96%), Postives = 198/216 (91.67%), Query Frame = 0

Query: 1   MANVIMASSKPLSPP-SISTK-PKFALPLPKTLNQLLLRKPQFAALSSSTLKSLSLFAAT 60
           MANVIMASSKPLSPP SIS K PKF++P   TL    L+ PQ  +LS+STLKSLSLFAAT
Sbjct: 48  MANVIMASSKPLSPPLSISNKTPKFSIPKTLTLIPQFLKNPQSISLSTSTLKSLSLFAAT 107

Query: 61  SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAAIKE 120
           SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMV LDKIYFSPLGKFMD+RDAAIKE
Sbjct: 108 SLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVGLDKIYFSPLGKFMDERDAAIKE 167

Query: 121 KLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRIDAEL 180
           KL+SVKDTS  VKQLDEQAAAVMRAARAEISAAL KMKKETQAEVE+KLAEGRKRIDAEL
Sbjct: 168 KLSSVKDTSEGVKQLDEQAAAVMRAARAEISAALNKMKKETQAEVEEKLAEGRKRIDAEL 227

Query: 181 QEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           QEALANLENQK ETIKSLDAQIAALSDEIVKKVLPV
Sbjct: 228 QEALANLENQKAETIKSLDAQIAALSDEIVKKVLPV 263

BLAST of MC08g0409 vs. ExPASy TrEMBL
Match: A0A6J1KTQ2 (ATP synthase subunit b', chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496318 PE=3 SV=1)

HSP 1 Score: 315 bits (806), Expect = 1.45e-106
Identity = 191/219 (87.21%), Postives = 202/219 (92.24%), Query Frame = 0

Query: 1   MANVIMASSKPLS-PPSI-STK-PKFALP--LPKTLNQLLLRKPQFAALSSSTLKSLSLF 60
           MANVIMASSKPLS PPSI STK PKF++P  L  TL   +L+ PQF +LS+STLKSLSL 
Sbjct: 1   MANVIMASSKPLSAPPSIASTKTPKFSIPKTLTLTLTPTILKNPQFLSLSTSTLKSLSLI 60

Query: 61  AATSLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRDAA 120
           AATSLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMV LDK+YFSPLGKFMD+RDAA
Sbjct: 61  AATSLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVGLDKLYFSPLGKFMDERDAA 120

Query: 121 IKEKLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKRID 180
           IKEKLNSVKDTS EVKQLDEQAAAVMRAARAEISAAL KMKKETQAEVE+KLAEGRK+ID
Sbjct: 121 IKEKLNSVKDTSEEVKQLDEQAAAVMRAARAEISAALNKMKKETQAEVEEKLAEGRKKID 180

Query: 181 AELQEALANLENQKEETIKSLDAQIAALSDEIVKKVLPV 214
           AELQEALA+LENQK ETIKSLDAQIAALSDEIVKKVLPV
Sbjct: 181 AELQEALASLENQKVETIKSLDAQIAALSDEIVKKVLPV 219

BLAST of MC08g0409 vs. TAIR 10
Match: AT4G32260.1 (ATPase, F0 complex, subunit B/B', bacterial/chloroplast )

HSP 1 Score: 240.4 bits (612), Expect = 1.4e-63
Identity = 149/220 (67.73%), Postives = 178/220 (80.91%), Query Frame = 0

Query: 2   ANVIMASSKPL------SPPSISTKPKFA-LP-LPKTLNQLLLRKPQFAALSSSTLKSLS 61
           AN IMASSKPL        P+    PKFA LP +PK+L      + +  +LSS+T KSL+
Sbjct: 3   ANSIMASSKPLISLSSNQQPNRVQIPKFAKLPQIPKSLTSSTDLRSKALSLSSATAKSLA 62

Query: 62  LFAATSLSFAPPSLAEEIEKAALFDFNLTLPIMMAQFLLLMVALDKIYFSPLGKFMDDRD 121
           L AA    FAPPS+AE +EKA LFDFNLTLPI++ +FL LM ALDK+Y+SPLG FMD RD
Sbjct: 63  LIAA----FAPPSMAEAMEKAQLFDFNLTLPIIVVEFLFLMFALDKVYYSPLGNFMDQRD 122

Query: 122 AAIKEKLNSVKDTSGEVKQLDEQAAAVMRAARAEISAALTKMKKETQAEVEQKLAEGRKR 181
           A+IKEKL SVKDTS EVK+LDEQAAAVMRAARAEI+AAL KMKKETQ EVE+KLAEGRK+
Sbjct: 123 ASIKEKLASVKDTSTEVKELDEQAAAVMRAARAEIAAALNKMKKETQVEVEEKLAEGRKK 182

Query: 182 IDAELQEALANLENQKEETIKSLDAQIAALSDEIVKKVLP 214
           ++ EL+EALA+LE+QKEETIK+LD+QIAALS++IVKKVLP
Sbjct: 183 VEEELKEALASLESQKEETIKALDSQIAALSEDIVKKVLP 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P318531.3e-5865.61ATP synthase subunit b', chloroplastic OS=Spinacia oleracea OX=3562 GN=ATPF2 PE=... [more]
A8J7856.5e-2638.78ATP synthase subunit b', chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=A... [more]
P480853.0e-1536.18ATP synthase B' chain, cyanelle OS=Cyanophora paradoxa OX=2762 GN=atpF2 PE=3 SV=... [more]
Q053672.6e-1431.76ATP synthase subunit b' OS=Synechococcus sp. (strain PCC 6716) OX=32048 GN=atpF2... [more]
Q112Z35.7e-1434.69ATP synthase subunit b' OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN... [more]
Match NameE-valueIdentityDescription
XP_022131427.18.74e-130100.00ATP synthase subunit b', chloroplastic [Momordica charantia][more]
XP_038886736.11.21e-10989.35ATP synthase subunit b', chloroplastic [Benincasa hispida][more]
XP_008445233.14.67e-10787.96PREDICTED: ATP synthase subunit b', chloroplastic [Cucumis melo][more]
XP_004138722.16.63e-10787.04ATP synthase subunit b', chloroplastic [Cucumis sativus] >KGN62960.1 hypothetica... [more]
KAA0064916.12.36e-10687.96ATP synthase subunit b' [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1BQZ64.23e-130100.00ATP synthase subunit b', chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A1S3BD202.26e-10787.96ATP synthase subunit b', chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488320 P... [more]
A0A0A0LM103.21e-10787.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G381850 PE=3 SV=1[more]
A0A5A7VF071.14e-10687.96ATP synthase subunit b OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A6J1KTQ21.45e-10687.21ATP synthase subunit b', chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114963... [more]
Match NameE-valueIdentityDescription
AT4G32260.11.4e-6367.73ATPase, F0 complex, subunit B/B', bacterial/chloroplast [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 116..136
NoneNo IPR availableCOILSCoilCoilcoord: 160..206
NoneNo IPR availablePANTHERPTHR33445:SF2ATP SYNTHASE SUBUNIT B', CHLOROPLASTICcoord: 1..213
NoneNo IPR availablePANTHERPTHR33445ATP SYNTHASE SUBUNIT B', CHLOROPLASTICcoord: 1..213
NoneNo IPR availableCDDcd06503ATP-synt_Fo_bcoord: 82..212
e-value: 2.87086E-16
score: 70.1591
IPR002146ATP synthase, F0 complex, subunit b/b', bacterial/chloroplastPFAMPF00430ATP-synt_Bcoord: 82..212
e-value: 5.0E-14
score: 52.5
IPR002146ATP synthase, F0 complex, subunit b/b', bacterial/chloroplastHAMAPMF_01398ATP_synth_b_bprimecoord: 77..214
score: 10.154982
IPR034679ATP synthase, F0 complex, subunit b'HAMAPMF_01399ATP_synth_bprimecoord: 76..213
score: 20.198795

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0409.1MC08g0409.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015986 ATP synthesis coupled proton transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009536 plastid
cellular_component GO:0045263 proton-transporting ATP synthase complex, coupling factor F(o)
molecular_function GO:0015078 proton transmembrane transporter activity