MC08g0380 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0380
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAmmonium transporter
LocationMC08: 3030971 .. 3033499 (+)
RNA-Seq ExpressionMC08g0380
SyntenyMC08g0380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGATCATAGTTCCTCTACCAGCGAATCTATTGGAAGACGCAGCGGACCCGGGATGGATGAGCAAGGGCGACAACGCGTGGCAGATGACGGCGGCGACGCTGGTGGGGCTGCAGAGCGTGCCGGGGCTGATAATCCTGTACGGCGGGGCGGTGAAGAAGAAGTGGGCGATCAACTCGGCGTTCATGGCGCTGTACGCGTTCGCGTGCGTGCTGGTGTGCTGGGTCGGGTGGGGGTACCGGCTGTCGTTTGGGGACAAGCTGGTGCCGTTTTGGGGGAAGCTCAACGTGGCGCTGGACCAGAAGTTTCTGCTGGACCCGGCCTTCAGCGGCAAGTTCCCCTCCGCGACCATGGTGTTCTTCCAGTTCGTGTTCGCCGCGATCACTCTGGTCCTCATCGCGGGGGCTCTCCTGGGCCGGATGAATTTCTATGCCTGGATGCTCTTCGTGCCGCTCTGGCTCACCTTCTCCTATACCTTTGTGGCGTTCAGTATTTGGAGCCCTAATGGGTTCTTCTATAAGATGGGGCTCATTGATTATTCCGGTGGCTATGTCATTCATTTGTCCTCTGGAGTTGCCGGCTTCACTGCCGCTTACTGGGTACGCCCTTTGACTCTTCGTCCACTCTTCCAAATTTGATAGCTACTTTATTTTTAAAAAAAATATATTCAAATACGGACGTGAAGATAAGATTTGATCCTTACCATAGTAATTGTTCTTACCACTATTTATCAATTTTTAAGGGGGTGAAGATAAGATTTGGTATTGTTGTTTAATATCTTTGTTTATTGATATCCTTTATGGTCAACTCCCGTGCTGTTTTTTTTTTTTCAAATGAATTATAAAATATAAGCCTAGTGTAAAATTGACAAGGCTTCGAAGACAAATATGACAATAGATTTACTTTAGTTCAAATTTTATAATTTGAGAGATTTGTTTAATGGTTTATTTTGTATATTAGTGATCGAGCTTAATTTTGCAAAAGAGTGATTTTGATTGAAGTGAGTGTTTTTAAAAAGATTTAGACATTGATTTTTTTTATTAAAATTGATGGGCAAACGAAATAATTTAGATAAAAGGTAAAAATTGTTGAGTCACATGTCTAAATCTATGATCTAGAATGATGAAATTAGGAGTGTATAGTAAAAACTGATACATCCTATAGTAAATATTGTTTTTTATTTTCTTGTAAAATTAAAACACTTTTTATTAACTATAACATAAGTAGGAGACAAGAAAAAAAAAACGTAAAATTTTGAGAATTTGTTTTAGAAAACAGAAACCTATATACATATATTTCTCAAAACATAAAAACTAACACATAATGAGAAACATCAAAAGAGACGAAAACTTTGCCATGCTAAAAATAATAGGACACAATAATAAAACTTAGAGGTGCGTATCTTCTAACTCACAATTGTTATAAAATTATTGACAAATGTTTATTTTATTCAAAAAGGGACAATTGTTTATTTTATTAAAAATTTGACGAGATTTTTTTATTTTCTCTCCATTTCTTTCATGCATGCATATCTTTGATATCAATTTTTTTAATAGTGATAAATAAGAAATATCTACTTATCAATAAATATTTAAGTTTGGCATAAAAAACTATCGTTAAAGAAAAATAAACTTGTTTTTAAAAAAGAAAAAAATTTAAAATAATCTTGAAAATACTTAAAAGTTCTTACCATCTCCCTATACGGACTATCATTGAATGTGTATTAGGTTGGCCCACGACTCTCGAAAGATCGGGAGAGCTTCCCACCGAACAACATCCTTCTAATGCTGGCGGGAGCCGGGTTACTATGGATGGGGTGGACCGGGTTCAACGGCGGAGACCCTTATGCGGCCAACATCGACGCCTCCCTCGCGGTACTCAACACCCACGTCTGCACCGCGACCAGCCTGCTGACATGGCTGATCTTCGACATCGTGTTCTTCCGTAAACCCTCGGTGATCGGCGCTGTCCAAGGCATGATCACCGGCCTGGTATGCATCACCCCGGCCGCCGGCCTGGTTCAAGGATGGGCCGCCATAATCATGGGAATGCTATCAGGGTCCATCCCGTGGTTCACCATGATGGTGGTCCACAAGAAATCCCCGGTCCTCCAAAAGGTGGACGACACAATGGCCGTGTTCCACACCCACGCCATCGCGGGCAGCCTTGGCGGCATCCTCAGCGGCATCTTCGCCGAGCCGCGCCTGAACTCCATCTACTACGGCGCGTACAACAAATACGTGGGCCTGTTCTACGGATTCAGCACGGCCCAAGTGGGGCTCGGCTTCCGCCAACTCGGCGTCCAACTCCTCGGCATCATATTTGTGACGTTGATCAATGTCATCGTCACCACCATCATCTGTGTGGCTCTCCAGTACATCGTGCCGCTACGGATGACGGAGGAGGACATGGCCATCGGCGACGAGGCCGCACACGGCGAAGAGGCTTACGCCATCTGGGGGCAGGGGGAGAAGCTTGATAACTCTAAGTATGACATTGAGATGCCTGCAAAGCACAGACACGCAGCTGAA

mRNA sequence

TCGATCATAGTTCCTCTACCAGCGAATCTATTGGAAGACGCAGCGGACCCGGGATGGATGAGCAAGGGCGACAACGCGTGGCAGATGACGGCGGCGACGCTGGTGGGGCTGCAGAGCGTGCCGGGGCTGATAATCCTGTACGGCGGGGCGGTGAAGAAGAAGTGGGCGATCAACTCGGCGTTCATGGCGCTGTACGCGTTCGCGTGCGTGCTGGTGTGCTGGGTCGGGTGGGGGTACCGGCTGTCGTTTGGGGACAAGCTGGTGCCGTTTTGGGGGAAGCTCAACGTGGCGCTGGACCAGAAGTTTCTGCTGGACCCGGCCTTCAGCGGCAAGTTCCCCTCCGCGACCATGGTGTTCTTCCAGTTCGTGTTCGCCGCGATCACTCTGGTCCTCATCGCGGGGGCTCTCCTGGGCCGGATGAATTTCTATGCCTGGATGCTCTTCGTGCCGCTCTGGCTCACCTTCTCCTATACCTTTGTGGCGTTCAGTATTTGGAGCCCTAATGGGTTCTTCTATAAGATGGGGCTCATTGATTATTCCGGTGGCTATGTCATTCATTTGTCCTCTGGAGTTGCCGGCTTCACTGCCGCTTACTGGGTTGGCCCACGACTCTCGAAAGATCGGGAGAGCTTCCCACCGAACAACATCCTTCTAATGCTGGCGGGAGCCGGGTTACTATGGATGGGGTGGACCGGGTTCAACGGCGGAGACCCTTATGCGGCCAACATCGACGCCTCCCTCGCGGTACTCAACACCCACGTCTGCACCGCGACCAGCCTGCTGACATGGCTGATCTTCGACATCGTGTTCTTCCGTAAACCCTCGGTGATCGGCGCTGTCCAAGGCATGATCACCGGCCTGGTATGCATCACCCCGGCCGCCGGCCTGGTTCAAGGATGGGCCGCCATAATCATGGGAATGCTATCAGGGTCCATCCCGTGGTTCACCATGATGGTGGTCCACAAGAAATCCCCGGTCCTCCAAAAGGTGGACGACACAATGGCCGTGTTCCACACCCACGCCATCGCGGGCAGCCTTGGCGGCATCCTCAGCGGCATCTTCGCCGAGCCGCGCCTGAACTCCATCTACTACGGCGCGTACAACAAATACGTGGGCCTGTTCTACGGATTCAGCACGGCCCAAGTGGGGCTCGGCTTCCGCCAACTCGGCGTCCAACTCCTCGGCATCATATTTGTGACGTTGATCAATGTCATCGTCACCACCATCATCTGTGTGGCTCTCCAGTACATCGTGCCGCTACGGATGACGGAGGAGGACATGGCCATCGGCGACGAGGCCGCACACGGCGAAGAGGCTTACGCCATCTGGGGGCAGGGGGAGAAGCTTGATAACTCTAAGTATGACATTGAGATGCCTGCAAAGCACAGACACGCAGCTGAA

Coding sequence (CDS)

TCGATCATAGTTCCTCTACCAGCGAATCTATTGGAAGACGCAGCGGACCCGGGATGGATGAGCAAGGGCGACAACGCGTGGCAGATGACGGCGGCGACGCTGGTGGGGCTGCAGAGCGTGCCGGGGCTGATAATCCTGTACGGCGGGGCGGTGAAGAAGAAGTGGGCGATCAACTCGGCGTTCATGGCGCTGTACGCGTTCGCGTGCGTGCTGGTGTGCTGGGTCGGGTGGGGGTACCGGCTGTCGTTTGGGGACAAGCTGGTGCCGTTTTGGGGGAAGCTCAACGTGGCGCTGGACCAGAAGTTTCTGCTGGACCCGGCCTTCAGCGGCAAGTTCCCCTCCGCGACCATGGTGTTCTTCCAGTTCGTGTTCGCCGCGATCACTCTGGTCCTCATCGCGGGGGCTCTCCTGGGCCGGATGAATTTCTATGCCTGGATGCTCTTCGTGCCGCTCTGGCTCACCTTCTCCTATACCTTTGTGGCGTTCAGTATTTGGAGCCCTAATGGGTTCTTCTATAAGATGGGGCTCATTGATTATTCCGGTGGCTATGTCATTCATTTGTCCTCTGGAGTTGCCGGCTTCACTGCCGCTTACTGGGTTGGCCCACGACTCTCGAAAGATCGGGAGAGCTTCCCACCGAACAACATCCTTCTAATGCTGGCGGGAGCCGGGTTACTATGGATGGGGTGGACCGGGTTCAACGGCGGAGACCCTTATGCGGCCAACATCGACGCCTCCCTCGCGGTACTCAACACCCACGTCTGCACCGCGACCAGCCTGCTGACATGGCTGATCTTCGACATCGTGTTCTTCCGTAAACCCTCGGTGATCGGCGCTGTCCAAGGCATGATCACCGGCCTGGTATGCATCACCCCGGCCGCCGGCCTGGTTCAAGGATGGGCCGCCATAATCATGGGAATGCTATCAGGGTCCATCCCGTGGTTCACCATGATGGTGGTCCACAAGAAATCCCCGGTCCTCCAAAAGGTGGACGACACAATGGCCGTGTTCCACACCCACGCCATCGCGGGCAGCCTTGGCGGCATCCTCAGCGGCATCTTCGCCGAGCCGCGCCTGAACTCCATCTACTACGGCGCGTACAACAAATACGTGGGCCTGTTCTACGGATTCAGCACGGCCCAAGTGGGGCTCGGCTTCCGCCAACTCGGCGTCCAACTCCTCGGCATCATATTTGTGACGTTGATCAATGTCATCGTCACCACCATCATCTGTGTGGCTCTCCAGTACATCGTGCCGCTACGGATGACGGAGGAGGACATGGCCATCGGCGACGAGGCCGCACACGGCGAAGAGGCTTACGCCATCTGGGGGCAGGGGGAGAAGCTTGATAACTCTAAGTATGACATTGAGATGCCTGCAAAGCACAGACACGCAGCTGAA

Protein sequence

SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE
Homology
BLAST of MC08g0380 vs. ExPASy Swiss-Prot
Match: A0A072VHJ1 (Ammonium transporter 2 member 5 OS=Medicago truncatula OX=3880 GN=AMT2-5 PE=2 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 1.6e-210
Identity = 347/449 (77.28%), Postives = 396/449 (88.20%), Query Frame = 0

Query: 4   VPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMA 63
           VP P+NLL   + P W+SK DNAWQ+ AATLVG+QSVPGLIILYGGAVKKKWA+NSAFM+
Sbjct: 4   VPFPSNLLPSPSSPEWLSKADNAWQLMAATLVGMQSVPGLIILYGGAVKKKWAVNSAFMS 63

Query: 64  LYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFV 123
           LYAFACV  CWV W YR+SFGD L PFWGK  +ALD+K+L   AF G FP+ATM++FQ V
Sbjct: 64  LYAFACVFFCWVFWAYRMSFGDTLFPFWGKPALALDEKYLFKQAFLGAFPNATMIYFQCV 123

Query: 124 FAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGY 183
           FAAITL+LIAGA+LGRMNFYAWM+FVPLWLTFSYTF AFSIWS NGF  KMG+IDYSGGY
Sbjct: 124 FAAITLILIAGAVLGRMNFYAWMMFVPLWLTFSYTFTAFSIWSTNGFLAKMGIIDYSGGY 183

Query: 184 VIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANI 243
           VIHLSSGVAGFTAAYWVGPRL+KDRE FPPNN+LLMLAGAGLLWMGWTGFNGGDPY+  +
Sbjct: 184 VIHLSSGVAGFTAAYWVGPRLNKDRERFPPNNLLLMLAGAGLLWMGWTGFNGGDPYSVGL 243

Query: 244 DASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAI 303
           DASLAVLNTH CTATSLLTW+  D++FFRKPSVIGAVQGMITGLVCITPAAG+VQGWAA+
Sbjct: 244 DASLAVLNTHACTATSLLTWVFLDVIFFRKPSVIGAVQGMITGLVCITPAAGVVQGWAAL 303

Query: 304 IMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIY 363
           IMG+ SGSIPWFTMMV+HK+S +LQKVDDTMAV HTHAIAGSLGGIL+G+FAEP+LNS++
Sbjct: 304 IMGLFSGSIPWFTMMVIHKRSKLLQKVDDTMAVLHTHAIAGSLGGILTGLFAEPKLNSLF 363

Query: 364 YGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLRMT 423
           YG YNKYVGLFYG        G RQ+G+QLLGI+FV  +NV+ T++IC+ ++  VPLRM+
Sbjct: 364 YGVYNKYVGLFYGIQMNMASTGRRQIGIQLLGILFVIFVNVVSTSLICLFIRLFVPLRMS 423

Query: 424 EEDMAIGDEAAHGEEAYAIWGQGEKLDNS 453
           EEDM IGDEAAHGEEAYAIWGQG+K +NS
Sbjct: 424 EEDMEIGDEAAHGEEAYAIWGQGDKHENS 452

BLAST of MC08g0380 vs. ExPASy Swiss-Prot
Match: Q84KJ6 (Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-1 PE=2 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.2e-170
Identity = 299/463 (64.58%), Postives = 358/463 (77.32%), Query Frame = 0

Query: 15  ADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCW 74
           A P W++KGDNAWQM +ATLVG+QSVPGL+ILYG  VKKKWA+NSAFMALYAFA V +CW
Sbjct: 18  AVPEWLNKGDNAWQMISATLVGMQSVPGLVILYGSIVKKKWAVNSAFMALYAFAAVWLCW 77

Query: 75  VGWGYRLSFGDKLVPFWGKLNVALDQKFLL-----------------------DPAFSGK 134
           V WGY +SFG KL+PFWGK   AL Q FLL                        P  +  
Sbjct: 78  VTWGYNMSFGHKLLPFWGKARPALGQSFLLAQAVLPQTTQFYKGGGGADAVVETPWVNPL 137

Query: 135 FPSATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF 194
           +P ATMV+FQ VFAAITL+L+AG+LLGRMN  AWMLFVPLWLTFSYT  AFS+W   GF 
Sbjct: 138 YPMATMVYFQCVFAAITLILLAGSLLGRMNIKAWMLFVPLWLTFSYTVGAFSLWG-GGFL 197

Query: 195 YKMGLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWT 254
           +  G++DYSGGYVIHLSSGVAGFTAAYWVGPR +KDRE FPPNN+LLML GAG+LWMGW 
Sbjct: 198 FHWGVMDYSGGYVIHLSSGVAGFTAAYWVGPRSTKDRERFPPNNVLLMLTGAGILWMGWA 257

Query: 255 GFNGGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCIT 314
           GFNGGDPY+ANID+SLAVLNT++C ATSLL W   D++FF+KPSVIGAVQGMITGLVCIT
Sbjct: 258 GFNGGDPYSANIDSSLAVLNTNICAATSLLVWTCLDVIFFKKPSVIGAVQGMITGLVCIT 317

Query: 315 PAAGLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILS 374
           P AGLVQGWAAI+MG+LSGSIPWFTMMVVHK+S +LQ+VDDT+ VFHTHA+AG LGG  +
Sbjct: 318 PGAGLVQGWAAIVMGILSGSIPWFTMMVVHKRSRLLQQVDDTLGVFHTHAVAGFLGGATT 377

Query: 375 GIFAEPRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIIC 434
           G+FAEP L S++    N     + G        G  Q   Q+ G +F+   NV+VT+++C
Sbjct: 378 GLFAEPVLCSLFLPVTNSRGAFYPGRG------GGLQFVRQVAGALFIICWNVVVTSLVC 437

Query: 435 VALQYIVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKY 455
           +A++ +VPLRM EE++AIGD+A HGEEAYA+WG GEK D++K+
Sbjct: 438 LAVRAVVPLRMPEEELAIGDDAVHGEEAYALWGDGEKYDSTKH 473

BLAST of MC08g0380 vs. ExPASy Swiss-Prot
Match: G7L1W7 (Ammonium transporter 2 member 4 OS=Medicago truncatula OX=3880 GN=AMT2-4 PE=2 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 2.3e-169
Identity = 291/453 (64.24%), Postives = 364/453 (80.35%), Query Frame = 0

Query: 6   LPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALY 65
           LP+NLL D A P WM+KGDNAWQ+TAAT+VGLQS+PGL+ILYG  VKK WAINSAFMA Y
Sbjct: 3   LPSNLLPDEASPEWMNKGDNAWQLTAATMVGLQSIPGLVILYGSLVKKTWAINSAFMAFY 62

Query: 66  AFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFVFA 125
           AFA VL+CWV W Y++SFG+K+V F GK NVALD+KFLL  AF G FP+ATMVF+Q VFA
Sbjct: 63  AFASVLLCWVSWAYQMSFGEKMVFFLGKPNVALDEKFLLGKAFLGNFPNATMVFYQGVFA 122

Query: 126 AITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGYVI 185
            +TL+LIAGALLGRMN  AWMLFVPLW+TFSYT VAFSIW P+G+  K G+ID++GGYVI
Sbjct: 123 GLTLILIAGALLGRMNIRAWMLFVPLWVTFSYTVVAFSIWCPDGWLAKRGVIDFAGGYVI 182

Query: 186 HLSSGVAGFTAAYWVGPRLSKDRESFP--PNNILLMLAGAGLLWMGWTGFNGGDPYAANI 245
           HLS+GVAGFTAAYWVGPR  KDRE+FP   NN++++LAGAGLLWMGW+GFNGG P+ A+ 
Sbjct: 183 HLSAGVAGFTAAYWVGPRADKDRETFPAATNNMIMVLAGAGLLWMGWSGFNGGAPFVAST 242

Query: 246 DASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAI 305
            ASLA+LNTHVCTA S+  W++ D  +F KP+V GAVQGMITGLVCITPAAG+VQGWAAI
Sbjct: 243 IASLAILNTHVCTAASITVWVMLDTFYFGKPTVFGAVQGMITGLVCITPAAGVVQGWAAI 302

Query: 306 IMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIY 365
           +MG +SGSIPW+TMMV+H K   L+K+DD MAVFHTHAIAG+LGGIL+G FA P+L  ++
Sbjct: 303 LMGFISGSIPWYTMMVLHNKVNFLKKIDDPMAVFHTHAIAGALGGILTGFFAVPKLCRLF 362

Query: 366 YGA--YNKYVGLFYGF-STAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 425
           Y    + KY+GL YG  +      G +Q+ +Q+  I+FV   NV++T++IC+ ++ IVPL
Sbjct: 363 YMVPDWEKYIGLAYGLQNKGATQAGLKQMVIQIEAIVFVICYNVLMTSLICLIVRVIVPL 422

Query: 426 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSK 454
           R+  + + +GD+A HGE+A+A+  +  K  N K
Sbjct: 423 RLNGDALQMGDKAIHGEDAFALHSEATKFVNIK 455

BLAST of MC08g0380 vs. ExPASy Swiss-Prot
Match: G7LAA8 (Ammonium transporter 2 member 3 OS=Medicago truncatula OX=3880 GN=AMT2-3 PE=2 SV=2)

HSP 1 Score: 589.7 bits (1519), Expect = 2.8e-167
Identity = 289/445 (64.94%), Postives = 350/445 (78.65%), Query Frame = 0

Query: 6   LPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALY 65
           LP +LL + A P W +K DNAWQ+TAATLVGLQ+VPGL+ILYG  VKKKWA+NSAFMALY
Sbjct: 12  LPESLLPNDASPEWNNKADNAWQLTAATLVGLQTVPGLVILYGSMVKKKWAVNSAFMALY 71

Query: 66  AFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKF-PSATMVFFQFVF 125
           AFA VLVCWV W + ++FG KL+PF GK N AL QKFLL  A +  + P A  VF+QF F
Sbjct: 72  AFAAVLVCWVLWAHHMAFGTKLLPFVGKPNFALSQKFLLSKASTNYYLPMADFVFYQFAF 131

Query: 126 AAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGYV 185
           AAITLVL+ G+LLGRMNFYAWMLFVPLWLT SYT  AF+IW  NGF  +  +IDY+GG+V
Sbjct: 132 AAITLVLLGGSLLGRMNFYAWMLFVPLWLTLSYTVGAFTIWG-NGFL-EGKIIDYAGGFV 191

Query: 186 IHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANID 245
           IHLSSGVAGFTAAYWVGPR S DR++FPPNNI+ ML GAG LWMGWTGFNGG P+     
Sbjct: 192 IHLSSGVAGFTAAYWVGPRTSNDRQNFPPNNIIHMLGGAGFLWMGWTGFNGGAPFQVGEI 251

Query: 246 ASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAII 305
            SLA+ NTH+CTATS+L W+  D+  ++K S+IG+VQGM+TGLVCITP AGLV  WAAI+
Sbjct: 252 TSLAIFNTHLCTATSILVWISLDMAVYKKGSLIGSVQGMMTGLVCITPGAGLVDPWAAIL 311

Query: 306 MGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIYY 365
           MG LSGSIPW+TMMV+HKKSP  Q VDDT+ VFHTHA+AG LGGILSG+FA+P+L  I Y
Sbjct: 312 MGALSGSIPWYTMMVLHKKSPFFQSVDDTLGVFHTHAVAGILGGILSGVFAKPKLLRILY 371

Query: 366 GAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLRMTE 425
           G Y    GL Y +    +G G +Q+  QLLG +F+T+ NV++T++IC+ L   V LRM E
Sbjct: 372 GPYGS--GLLYSYFDDNIGQGIKQMWYQLLGAVFITIWNVVITSLICILLNRFVNLRMQE 431

Query: 426 EDMAIGDEAAHGEEAYAIWGQGEKL 450
           ED+ +GD+AAHGEEAY +WG GE++
Sbjct: 432 EDLEVGDDAAHGEEAYVLWGDGERM 452

BLAST of MC08g0380 vs. ExPASy Swiss-Prot
Match: Q69T29 (Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-3 PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 2.8e-167
Identity = 288/459 (62.75%), Postives = 355/459 (77.34%), Query Frame = 0

Query: 15  ADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCW 74
           + P W++KGDNAWQMT+ATLVGLQS+PGL+ILYG  VKKKWAINSAFMALYAFA V +CW
Sbjct: 15  SSPDWLNKGDNAWQMTSATLVGLQSMPGLVILYGSIVKKKWAINSAFMALYAFAAVWICW 74

Query: 75  VGWGYRLSFGDKLVPFWGKLNVALDQKFLL------------------DPAFSGKFPSAT 134
           V W Y +SFGD+L+PFWGK   AL Q FL+                   P     +P AT
Sbjct: 75  VVWAYNMSFGDRLLPFWGKARPALGQSFLVAQSELTATAIRYHNGSAEAPMLKPLYPVAT 134

Query: 135 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 194
           MV+FQ +FA+IT++++AG+LLGRMN  AWM FVPLW+TFSYT  AFS+W   GF ++ G+
Sbjct: 135 MVYFQCMFASITIIILAGSLLGRMNIKAWMAFVPLWITFSYTVCAFSLWG-GGFLFQWGV 194

Query: 195 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 254
           IDYSGGYVIHLSSG+AG TAAYWVGPR + DRE FPPNNILL+LAGAGLLW+GWTGFNGG
Sbjct: 195 IDYSGGYVIHLSSGIAGLTAAYWVGPRSASDRERFPPNNILLVLAGAGLLWLGWTGFNGG 254

Query: 255 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 314
           DPY+ANID+S+AVLNTH+C +TSLL W I D+ FF KPSVIGAVQGMITGLVCITP AGL
Sbjct: 255 DPYSANIDSSMAVLNTHICASTSLLVWTILDVFFFGKPSVIGAVQGMITGLVCITPGAGL 314

Query: 315 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 374
           VQGWAAI+MG+LSGSIPW+TMMV+HKK   +Q++DDT+ VFHTHA+AG LGG  +G+FAE
Sbjct: 315 VQGWAAIVMGILSGSIPWYTMMVLHKKWSFMQRIDDTLGVFHTHAVAGFLGGATTGLFAE 374

Query: 375 PRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 434
           P L S++    +   G FYG      G G  Q G Q+ G +FVT  N+++T+IICV +  
Sbjct: 375 PILCSLFLSIPDS-KGAFYG------GPGGSQFGKQIAGALFVTAWNIVITSIICVIISL 434

Query: 435 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYD 456
           I+PLR+ ++++ IGD+A HGEEAYAIW +GE  D + ++
Sbjct: 435 ILPLRIADQELLIGDDAVHGEEAYAIWAEGELNDMTHHN 465

BLAST of MC08g0380 vs. NCBI nr
Match: XP_022131630.1 (ammonium transporter 3 member 1 [Momordica charantia])

HSP 1 Score: 933 bits (2411), Expect = 0.0
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 5   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 64

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF
Sbjct: 65  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 124

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS
Sbjct: 125 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 184

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 185 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 244

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW
Sbjct: 245 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 304

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 305 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 364

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
           SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL
Sbjct: 365 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 424

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE 467
           RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE
Sbjct: 425 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE 471

BLAST of MC08g0380 vs. NCBI nr
Match: XP_022962592.1 (ammonium transporter 3 member 1-like [Cucurbita moschata] >KAG6598591.1 Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029524.1 Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 875 bits (2260), Expect = 0.0
Identity = 433/467 (92.72%), Postives = 455/467 (97.43%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           S+IVPLP+NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFGDKL+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
            +YYGAYNKYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHA 465
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE+P  AKHRHA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHA 468

BLAST of MC08g0380 vs. NCBI nr
Match: XP_023546976.1 (ammonium transporter 3 member 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 433/467 (92.72%), Postives = 454/467 (97.22%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           S+IVPLP NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPPNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFGDKL+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
            +YYGAYNKYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHA 465
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE+P  AKHRHA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHA 468

BLAST of MC08g0380 vs. NCBI nr
Match: XP_022996665.1 (ammonium transporter 3 member 1-like [Cucurbita maxima])

HSP 1 Score: 872 bits (2253), Expect = 0.0
Identity = 432/466 (92.70%), Postives = 453/466 (97.21%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           S+IVPLP+NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFGD+L+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDRLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
            +YYGAYNKYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPA--KHRH 464
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D SKYDIE+PA  KHRH
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDTSKYDIEVPAVAKHRH 467

BLAST of MC08g0380 vs. NCBI nr
Match: XP_004138867.1 (ammonium transporter 3 member 1 [Cucumis sativus] >KAE8652192.1 hypothetical protein Csa_021807 [Cucumis sativus])

HSP 1 Score: 866 bits (2237), Expect = 1.61e-315
Identity = 428/468 (91.45%), Postives = 450/468 (96.15%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           +IIVPLPANL+ D  DPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 3   NIIVPLPANLVTDVGDPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 62

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFG+KL PFWGKLNVALDQ++LL P F+GKFP+ATMVFF
Sbjct: 63  FMALYAFACVLVCWVGWGYRLSFGEKLAPFWGKLNVALDQEYLLAPGFAGKFPTATMVFF 122

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSP GFF KMGLIDYS
Sbjct: 123 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPGGFFAKMGLIDYS 182

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 183 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 242

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 243 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 302

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAV HTHAIAGSLGGILSGIFAEP LN
Sbjct: 303 AAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPHLN 362

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
           +I+YG YNKY+GLFYGF+T QV LGFRQLGVQLLGIIFVTL+N+IVT++ICV LQYIVPL
Sbjct: 363 NIFYGNYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIIFVTLLNIIVTSLICVLLQYIVPL 422

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHAA 466
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE P  AKHRHAA
Sbjct: 423 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEAPVSAKHRHAA 470

BLAST of MC08g0380 vs. ExPASy TrEMBL
Match: A0A6J1BQ83 (Ammonium transporter OS=Momordica charantia OX=3673 GN=LOC111004759 PE=3 SV=1)

HSP 1 Score: 933 bits (2411), Expect = 0.0
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 5   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 64

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF
Sbjct: 65  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 124

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS
Sbjct: 125 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 184

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 185 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 244

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW
Sbjct: 245 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 304

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 305 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 364

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
           SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL
Sbjct: 365 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 424

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE 467
           RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE
Sbjct: 425 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE 471

BLAST of MC08g0380 vs. ExPASy TrEMBL
Match: A0A6J1HD34 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111462995 PE=3 SV=1)

HSP 1 Score: 875 bits (2260), Expect = 0.0
Identity = 433/467 (92.72%), Postives = 455/467 (97.43%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           S+IVPLP+NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFGDKL+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
            +YYGAYNKYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHA 465
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE+P  AKHRHA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHA 468

BLAST of MC08g0380 vs. ExPASy TrEMBL
Match: A0A6J1K7F6 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111491842 PE=3 SV=1)

HSP 1 Score: 872 bits (2253), Expect = 0.0
Identity = 432/466 (92.70%), Postives = 453/466 (97.21%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           S+IVPLP+NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFGD+L+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDRLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
            +YYGAYNKYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPA--KHRH 464
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D SKYDIE+PA  KHRH
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDTSKYDIEVPAVAKHRH 467

BLAST of MC08g0380 vs. ExPASy TrEMBL
Match: A0A1S3BCV9 (Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103488277 PE=3 SV=1)

HSP 1 Score: 862 bits (2226), Expect = 2.26e-314
Identity = 426/469 (90.83%), Postives = 450/469 (95.95%), Query Frame = 0

Query: 1   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 60
           +IIVPLPAN++ D  DPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 3   NIIVPLPANVVPDIGDPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 62

Query: 61  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 120
           FMALYAFACVLVCWVGWGYRLSFG+KLVPFWGKLNVALDQ++LL   F+GKFP+ATMVFF
Sbjct: 63  FMALYAFACVLVCWVGWGYRLSFGEKLVPFWGKLNVALDQEYLLQQGFAGKFPAATMVFF 122

Query: 121 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 180
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSP GFF KMGLIDYS
Sbjct: 123 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPEGFFAKMGLIDYS 182

Query: 181 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 240
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 183 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 242

Query: 241 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 300
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 243 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 302

Query: 301 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 360
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAV HTHAIAGSLGGILSGIFAEP LN
Sbjct: 303 AAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPHLN 362

Query: 361 SIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 420
           +I+YG YN+Y+GLFYGF+T QV LGFRQLGVQLLGIIFVTL+NVIVT++ICV LQYIVPL
Sbjct: 363 NIFYGNYNQYIGLFYGFNTKQVRLGFRQLGVQLLGIIFVTLLNVIVTSLICVLLQYIVPL 422

Query: 421 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP---AKHRHAA 466
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYD+E P   AKHRHAA
Sbjct: 423 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDVEAPIAAAKHRHAA 471

BLAST of MC08g0380 vs. ExPASy TrEMBL
Match: M5Y958 (Ammonium transporter OS=Prunus persica OX=3760 GN=AMT PE=2 SV=1)

HSP 1 Score: 767 bits (1980), Expect = 7.34e-277
Identity = 374/467 (80.09%), Postives = 421/467 (90.15%), Query Frame = 0

Query: 5   PLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMAL 64
           PLP+NLL D A+P WM+K DNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWA+NSAFMAL
Sbjct: 8   PLPSNLLPDPANPDWMNKADNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAVNSAFMAL 67

Query: 65  YAFACVLVCWVGWGYRLSFGDKL---VPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQ 124
           YAFACVLVCWVGWGY +SFG+ L   VPFWGK +VAL+QK+LL+ AFSGKFP+ATMV+FQ
Sbjct: 68  YAFACVLVCWVGWGYHMSFGETLIKGVPFWGKADVALNQKYLLEQAFSGKFPNATMVYFQ 127

Query: 125 FVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSG 184
           FVFAAITL+LIAGALLGRMNFYAWMLFVPLWLTFSYT  AFSIWSP GF +K GLIDYSG
Sbjct: 128 FVFAAITLILIAGALLGRMNFYAWMLFVPLWLTFSYTIGAFSIWSPKGFLFKKGLIDYSG 187

Query: 185 GYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAA 244
           G+VIHLSSGVAGFTAAYWVGPR +KDRE FPPNNILLML GAGLLWMGWTGFNGGDPY  
Sbjct: 188 GFVIHLSSGVAGFTAAYWVGPRSNKDRERFPPNNILLMLTGAGLLWMGWTGFNGGDPYVV 247

Query: 245 NIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWA 304
           ++DASLAVLNTHVCTATSLLTWL+ D++FFRKPSVIGAVQGMITGLVCITPAAG+VQGWA
Sbjct: 248 SVDASLAVLNTHVCTATSLLTWLLLDLIFFRKPSVIGAVQGMITGLVCITPAAGVVQGWA 307

Query: 305 AIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNS 364
           AI+MG+ SGSIPWFTMMVVHKKS +LQKVDDTMAV HTHAIAG+LGGIL+G+FA PRL  
Sbjct: 308 AILMGLFSGSIPWFTMMVVHKKSALLQKVDDTMAVLHTHAIAGALGGILTGLFAHPRLCF 367

Query: 365 IYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLR 424
           ++  +Y +YVGLFYGF    V  GFRQLG+QLLGI +V ++NV+VT++ICV +Q+IVPLR
Sbjct: 368 LFTTSYGQYVGLFYGFKMEVVHTGFRQLGIQLLGIFYVVILNVVVTSLICVLIQFIVPLR 427

Query: 425 MTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKY--DIEMPAKHRHAA 466
           M++ DM IGDEAAHGEEAYAIWGQGEKL+NS+Y  DIE  AK+RHAA
Sbjct: 428 MSDADMEIGDEAAHGEEAYAIWGQGEKLENSRYGYDIESSAKNRHAA 474

BLAST of MC08g0380 vs. TAIR 10
Match: AT2G38290.1 (ammonium transporter 2 )

HSP 1 Score: 555.4 bits (1430), Expect = 4.2e-158
Identity = 278/445 (62.47%), Postives = 345/445 (77.53%), Query Frame = 0

Query: 17  PGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVG 76
           P W++KGDNAWQ+TAATLVGLQS+PGL+ILY   VKKKWA+NSAFMALYAFA VL+CWV 
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 77  WGYRLSFGDKLVPFWGKLNVALDQKFLLDPA-------FSGKFPSATMVFFQFVFAAITL 136
             Y+++FG++L+PFWGK   A DQ +L   A        +  FP AT+V+FQF FAAIT 
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNVAAPYFPMATLVYFQFTFAAITT 132

Query: 137 VLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGYVIHLSS 196
           +L+AG++LGRMN  AWM FVPLWL FSYT  A+SIW   GF Y+ G+IDYSGGYVIHLSS
Sbjct: 133 ILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWG-GGFLYQWGVIDYSGGYVIHLSS 192

Query: 197 GVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANIDASLAV 256
           GVAGF AAYWVGPR   DRE FPPNN+LLMLAGAGLLWMGW+GFNGG PYAAN+ +S+AV
Sbjct: 193 GVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYAANLTSSIAV 252

Query: 257 LNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAIIMGMLS 316
           LNT++  ATSLL W   D++FF KPSVIGA+QGM+TGL  +TP AGL+Q WAAII+G++S
Sbjct: 253 LNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTWAAIIIGVVS 312

Query: 317 GSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIYYGAYNK 376
           G+ PW +MM++HKKS +LQKVDDT+AVF+THA+AG LGGI++G+FA P L  +       
Sbjct: 313 GTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDL-CVLVLPLPA 372

Query: 377 YVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLRMTEEDMAI 436
             G FYG      G G +QL  QL G  F+ + NV+ TTII +A++  +PLRM EE++ I
Sbjct: 373 TRGAFYG------GNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEELGI 432

Query: 437 GDEAAHGEEAYAIWGQGEKLDNSKY 455
           GD+AAHGEEAYA+WG GEK D +++
Sbjct: 433 GDDAAHGEEAYALWGDGEKFDATRH 449

BLAST of MC08g0380 vs. TAIR 10
Match: AT2G38290.2 (ammonium transporter 2 )

HSP 1 Score: 428.7 bits (1101), Expect = 6.0e-120
Identity = 215/340 (63.24%), Postives = 267/340 (78.53%), Query Frame = 0

Query: 115 ATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKM 174
           AT+V+FQF FAAIT +L+AG++LGRMN  AWM FVPLWL FSYT  A+SIW   GF Y+ 
Sbjct: 2   ATLVYFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWG-GGFLYQW 61

Query: 175 GLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFN 234
           G+IDYSGGYVIHLSSGVAGF AAYWVGPR   DRE FPPNN+LLMLAGAGLLWMGW+GFN
Sbjct: 62  GVIDYSGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFN 121

Query: 235 GGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAA 294
           GG PYAAN+ +S+AVLNT++  ATSLL W   D++FF KPSVIGA+QGM+TGL  +TP A
Sbjct: 122 GGAPYAANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGA 181

Query: 295 GLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIF 354
           GL+Q WAAII+G++SG+ PW +MM++HKKS +LQKVDDT+AVF+THA+AG LGGI++G+F
Sbjct: 182 GLIQTWAAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLF 241

Query: 355 AEPRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVAL 414
           A P L  +         G FYG      G G +QL  QL G  F+ + NV+ TTII +A+
Sbjct: 242 AHPDL-CVLVLPLPATRGAFYG------GNGGKQLLKQLAGAAFIAVWNVVSTTIILLAI 301

Query: 415 QYIVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKY 455
           +  +PLRM EE++ IGD+AAHGEEAYA+WG GEK D +++
Sbjct: 302 RVFIPLRMAEEELGIGDDAAHGEEAYALWGDGEKFDATRH 333

BLAST of MC08g0380 vs. TAIR 10
Match: AT3G24290.1 (ammonium transporter 1;5 )

HSP 1 Score: 112.8 bits (281), Expect = 7.2e-25
Identity = 121/446 (27.13%), Postives = 202/446 (45.29%), Query Frame = 0

Query: 24  DNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 83
           DN + + +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 84  GDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFY 143
           G+    F G+ N  L Q F   P  +  +   +   +Q+ FA     + +G++  R  F 
Sbjct: 106 GESSDGFIGRHNFGL-QNF---PTLTSDY---SFFLYQWAFAIAAAGITSGSIAERTKFV 165

Query: 144 AWMLFVPLWLTFSYTFVAFSIWSPNGF---------FYKMGLIDYSGGYVIHLSSGVAGF 203
           A++++      F Y  V+   WSP+G+          +  G ID++G  V+H+  G+AG 
Sbjct: 166 AYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSEDRLFGTGAIDFAGSGVVHMVGGIAGL 225

Query: 204 TAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD------PY--AA 263
             A   GPR+ +  +     +   ++  L++ G  LLW GW GFN G       PY   +
Sbjct: 226 WGALIEGPRIGRFPDGGHAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILIPYNSGS 285

Query: 264 NIDASLAVLNTHVCT----ATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLV 323
           N      +  T V T     T+ LT L    +     +V     G++ G   IT    +V
Sbjct: 286 NYGQWSGIGRTAVTTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGCSVV 345

Query: 324 QGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFA-E 383
             WAAI+ G ++  +     ++   K   L K DD +     H   G+ G I  G+FA E
Sbjct: 346 DPWAAIVCGFVASLV-----LIGCNKLAELLKYDDPLEAAQLHGGCGAWGLIFVGLFAKE 405

Query: 384 PRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGII-FVTLINVIVTTIICVALQ 441
             +N +Y  +  ++ GLF       +G G + LG QL+ II  V  ++  + T+  + L+
Sbjct: 406 KYINEVYGASPGRHYGLF-------MGGGGKLLGAQLVQIIVIVGWVSATMGTLFFI-LK 465

BLAST of MC08g0380 vs. TAIR 10
Match: AT3G24300.1 (ammonium transporter 1;3 )

HSP 1 Score: 110.2 bits (274), Expect = 4.7e-24
Identity = 118/451 (26.16%), Postives = 197/451 (43.68%), Query Frame = 0

Query: 24  DNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 83
           DN + + +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 84  GDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT----MVFFQFVFAAITLVLIAGALLGR 143
           G     F G+ N AL             FP+ T       +Q+ FA     + +G++  R
Sbjct: 106 GGSSEGFIGRHNFALRD-----------FPTPTADYSFFLYQWAFAIAAAGITSGSIAER 165

Query: 144 MNFYAWMLFVPLWLTFSYTFVAFSIWSPNGF----------FYKMGLIDYSGGYVIHLSS 203
             F A++++      F Y  V+   WSP+G+           +  G ID++G  V+H+  
Sbjct: 166 TQFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSADDRLFSTGAIDFAGSGVVHMVG 225

Query: 204 GVAGFTAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD------P 263
           G+AG   A   GPR  +  +     +   ++  L++ G  LLW GW GFN G       P
Sbjct: 226 GIAGLWGALIEGPRRGRFEKGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILVP 285

Query: 264 YAANID------ASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITP 323
           Y +  +           +NT +   T+ LT L    +     +V     G++ G   IT 
Sbjct: 286 YNSGSNYGQWSGIGRTAVNTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITA 345

Query: 324 AAGLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSG 383
              +V+ WAAI+ G ++       +++   K   L + DD +     H   G+ G I  G
Sbjct: 346 GCSVVEPWAAIVCGFMAS-----VVLIGCNKLAELVQYDDPLEAAQLHGGCGAWGLIFVG 405

Query: 384 IFA-EPRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGI-IFVTLINVIVTTII 441
           +FA E  LN +Y     +  GLF       +G G + LG QL+ I + V  ++  + T+ 
Sbjct: 406 LFAKEKYLNEVYGATPGRPYGLF-------MGGGGKLLGAQLVQILVIVGWVSATMGTLF 465

BLAST of MC08g0380 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 102.4 bits (254), Expect = 9.8e-22
Identity = 120/454 (26.43%), Postives = 197/454 (43.39%), Query Frame = 0

Query: 24  DNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 83
           DN + + +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 42  DNTYLLFSAYLVFSMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 101

Query: 84  GDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFY 143
           G     F GK    L       P  S  + +     +Q+ FA     + +G++  R  F 
Sbjct: 102 GSPSNGFIGKHYFGLKD----IPTASADYSN---FLYQWAFAIAAAGITSGSIAERTQFV 161

Query: 144 AWMLFVPLWLTFSYTFVAFSIWSPNGF----------FYKMGLIDYSGGYVIHLSSGVAG 203
           A++++      F Y  V+   WS +G+           +  G ID++G  V+H+  G+AG
Sbjct: 162 AYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLFSTGAIDFAGSGVVHMVGGIAG 221

Query: 204 FTAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD----------- 263
              A   GPRL +        +   ++  L++ G  LLW GW GFN G            
Sbjct: 222 LWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFNKILVTYETG 281

Query: 264 PYAANIDA-SLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 323
            Y     A     + T +   T+ LT L    +     +V     G++ G   IT    +
Sbjct: 282 TYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITGGCSV 341

Query: 324 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 383
           V+ WAAII G ++  +    ++  +K +  L K DD +     H   G+ G I + +FA+
Sbjct: 342 VEPWAAIICGFVAALV----LLGCNKLAEKL-KYDDPLEAAQLHGGCGAWGLIFTALFAQ 401

Query: 384 PR-LNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVT-LINVIVTTIICVAL 443
            + LN IY     +  GLF       +G G + LG QL+ II +T  ++  + T+  + L
Sbjct: 402 EKYLNQIYGNKPGRPHGLF-------MGGGGKLLGAQLIQIIVITGWVSATMGTLFFI-L 461

Query: 444 QYIVPLRMTEED-MAIGDEAAHGEEAYAIWGQGE 448
           + +  LR++ ED MA  D   HG  AY  +   E
Sbjct: 462 KKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDE 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A072VHJ11.6e-21077.28Ammonium transporter 2 member 5 OS=Medicago truncatula OX=3880 GN=AMT2-5 PE=2 SV... [more]
Q84KJ61.2e-17064.58Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
G7L1W72.3e-16964.24Ammonium transporter 2 member 4 OS=Medicago truncatula OX=3880 GN=AMT2-4 PE=2 SV... [more]
G7LAA82.8e-16764.94Ammonium transporter 2 member 3 OS=Medicago truncatula OX=3880 GN=AMT2-3 PE=2 SV... [more]
Q69T292.8e-16762.75Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
Match NameE-valueIdentityDescription
XP_022131630.10.0100.00ammonium transporter 3 member 1 [Momordica charantia][more]
XP_022962592.10.092.72ammonium transporter 3 member 1-like [Cucurbita moschata] >KAG6598591.1 Ammonium... [more]
XP_023546976.10.092.72ammonium transporter 3 member 1-like [Cucurbita pepo subsp. pepo][more]
XP_022996665.10.092.70ammonium transporter 3 member 1-like [Cucurbita maxima][more]
XP_004138867.11.61e-31591.45ammonium transporter 3 member 1 [Cucumis sativus] >KAE8652192.1 hypothetical pro... [more]
Match NameE-valueIdentityDescription
A0A6J1BQ830.0100.00Ammonium transporter OS=Momordica charantia OX=3673 GN=LOC111004759 PE=3 SV=1[more]
A0A6J1HD340.092.72Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111462995 PE=3 SV=1[more]
A0A6J1K7F60.092.70Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111491842 PE=3 SV=1[more]
A0A1S3BCV92.26e-31490.83Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103488277 PE=3 SV=1[more]
M5Y9587.34e-27780.09Ammonium transporter OS=Prunus persica OX=3760 GN=AMT PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38290.14.2e-15862.47ammonium transporter 2 [more]
AT2G38290.26.0e-12063.24ammonium transporter 2 [more]
AT3G24290.17.2e-2527.13ammonium transporter 1;5 [more]
AT3G24300.14.7e-2426.16ammonium transporter 1;3 [more]
AT4G13510.19.8e-2226.43ammonium transporter 1;1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002229Blood group Rhesus C/E/D polypeptidePRINTSPR00342RHESUSRHDcoord: 248..270
score: 27.54
coord: 217..235
score: 41.52
coord: 121..137
score: 25.49
IPR001905Ammonium transporterTIGRFAMTIGR00836TIGR00836coord: 24..440
e-value: 1.5E-113
score: 377.8
IPR029020Ammonium/urea transporterGENE3D1.10.3430.10Ammonium transporter AmtB like domainscoord: 10..444
e-value: 4.4E-124
score: 416.5
IPR024041Ammonium transporter AmtB-like domainPFAMPF00909Ammonium_transpcoord: 26..440
e-value: 1.2E-83
score: 281.1
NoneNo IPR availablePANTHERPTHR43029:SF7AMMONIUM TRANSPORTERcoord: 4..462
NoneNo IPR availablePANTHERPTHR43029AMMONIUM TRANSPORTER MEP2coord: 4..462
NoneNo IPR availableSUPERFAMILY111352Ammonium transportercoord: 19..422
IPR018047Ammonium transporter, conserved sitePROSITEPS01219AMMONIUM_TRANSPcoord: 178..203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0380.1MC08g0380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072488 ammonium transmembrane transport
biological_process GO:0019740 nitrogen utilization
biological_process GO:0015696 ammonium transport
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008519 ammonium transmembrane transporter activity