MC08g0348 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g0348 vs. NCBI nr
Match: XP_022131479.1 (uncharacterized protein DDB_G0271670 isoform X2 [Momordica charantia])

HSP 1 Score: 809 bits (2090), Expect = 9.54e-293
Identity = 465/516 (90.12%), Postives = 465/516 (90.12%), Query Frame = 0

Query: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS 60
           MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS
Sbjct: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS 60

Query: 61  SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR 120
           SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR
Sbjct: 61  SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR 120

Query: 121 SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT 180
           SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT
Sbjct: 121 SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT 180

Query: 181 SPKV---------------------------------------------------GGKPQ 240
           SPKV                                                   GGKPQ
Sbjct: 181 SPKVEPSYLERSSSAKTSSKVEPSHLERSSSSKSSSKVEPSHLERSSSSKSSSKVGGKPQ 240

Query: 241 QPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELK 300
           QPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELK
Sbjct: 241 QPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELK 300

Query: 301 KIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTE 360
           KIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTE
Sbjct: 301 KIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTE 360

Query: 361 TPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEG 420
           TPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEG
Sbjct: 361 TPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEG 420

Query: 421 IVVLDQKEVEDGENCKEEAEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAMYN 465
           IVVLDQKEVEDGENCKEEAEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAMYN
Sbjct: 421 IVVLDQKEVEDGENCKEEAEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAMYN 480

BLAST of MC08g0348 vs. NCBI nr
Match: XP_022131477.1 (uncharacterized protein DDB_G0271670 isoform X1 [Momordica charantia] >XP_022131478.1 uncharacterized protein DDB_G0271670 isoform X1 [Momordica charantia])

HSP 1 Score: 796 bits (2056), Expect = 5.05e-287
Identity = 465/550 (84.55%), Postives = 465/550 (84.55%), Query Frame = 0

Query: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS 60
           MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS
Sbjct: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS 60

Query: 61  SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR 120
           SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR
Sbjct: 61  SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR 120

Query: 121 SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT 180
           SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT
Sbjct: 121 SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT 180

Query: 181 SPKV-------------------------------------------------------- 240
           SPKV                                                        
Sbjct: 181 SPKVEPSYLERSSSAKTSSKVEPSHLERSSSSKSSSKVEPSHLERSSSAKTSSKVEPSHL 240

Query: 241 -----------------------------GGKPQQPVQNLRSGSNAIAKKSLRRESSNAG 300
                                        GGKPQQPVQNLRSGSNAIAKKSLRRESSNAG
Sbjct: 241 ERSSSSKSSSKVEPSHLERSSSSKSSSKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAG 300

Query: 301 SALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTDHV 360
           SALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTDHV
Sbjct: 301 SALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTDHV 360

Query: 361 EKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVG 420
           EKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVG
Sbjct: 361 EKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVG 420

Query: 421 EEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEVTEKVA 465
           EEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEVTEKVA
Sbjct: 421 EEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEVTEKVA 480

BLAST of MC08g0348 vs. NCBI nr
Match: KAG7029749.1 (hypothetical protein SDJN02_08091, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 419 bits (1076), Expect = 1.66e-139
Identity = 304/502 (60.56%), Postives = 340/502 (67.73%), Query Frame = 0

Query: 1   MATSRAS----FGKERRITAPTSLTIPNY--STKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+ITAP+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKA 120
           SSSS       SSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   PAPIPEGK 
Sbjct: 61  SSSS-------SSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSGGPAPIPEGKP 120

Query: 121 SLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLER 180
           SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ +  SS K G LER
Sbjct: 121 SLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFLER 180

Query: 181 SSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSAL-AEIVPKAKNIAVEQA 240
           S S     KVGGK QQ VQ L+S SN   +KSL+RESSNA SA  AE+VPK KN AVE  
Sbjct: 181 SGSKIY--KVGGKTQQSVQTLKSSSNV--RKSLKRESSNAASASSAEMVPKTKN-AVEHV 240

Query: 241 VHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRT-DHVEKKVDVLDKEEADAKHE 300
             +P  VLG NEE+LKKIECELD  LPDP PELDKE+ T D VEK++             
Sbjct: 241 GQTPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH------------ 300

Query: 301 AEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEEKP 360
              QPIQDT+I      +K   ETP   E + AK  IQE+ NRE+ EG GEEE K+EEKP
Sbjct: 301 -HHQPIQDTKIFKVVGSNKKVEETPGAAETDNAKSSIQEDENREQNEGEGEEEAKEEEKP 360

Query: 361 EIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKEE----- 420
           E I  EKE                +EEKS   P QEG++VL+ KEVEDG++ KEE     
Sbjct: 361 ERIEVEKEEVFGVVASEESRNNKNEEEKSHTDPIQEGVIVLEHKEVEDGQSSKEENHNST 420

Query: 421 ---AEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLEKR 465
              AE TEKVAAA     A AE  A   AK RQG  GRKESPA+YN VIEETASKLLEKR
Sbjct: 421 EKEAEATEKVAAA---ATATAEAAA---AKPRQGGPGRKESPAVYNHVIEETASKLLEKR 467

BLAST of MC08g0348 vs. NCBI nr
Match: XP_023546370.1 (cell wall protein RBR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418 bits (1074), Expect = 3.44e-139
Identity = 301/502 (59.96%), Postives = 336/502 (66.93%), Query Frame = 0

Query: 1   MATSRAS----FGKERRITAPTSLTIPNY--STKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+ITAP+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNVNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKA 120
           SSSS       SSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   P PIPEGK 
Sbjct: 61  SSSS-------SSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSAGPTPIPEGKP 120

Query: 121 SLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLER 180
           SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ T  SS K G LER
Sbjct: 121 SLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTTIVSSTKTGFLER 180

Query: 181 SSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSAL-AEIVPKAKNIAVEQA 240
           S S     KVGGK QQ VQ L+S SN   +KSL+RESSNA SA  AE+VPK KN AVE  
Sbjct: 181 SGSKIY--KVGGKTQQSVQTLKSSSNV--RKSLKRESSNAASASSAEMVPKTKN-AVEHV 240

Query: 241 VHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRT-DHVEKKVDVLDKEEADAKHE 300
             SP  VLG NEE+LKKIECELD  LPDP PELDKE+ T D VEK++             
Sbjct: 241 GQSPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH------------ 300

Query: 301 AEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEEKP 360
              QPIQDT+I      +K   ETP   E + AK  I+E+ NRE+ EG GEEE K+EEKP
Sbjct: 301 -HHQPIQDTKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKP 360

Query: 361 EIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKEE----- 420
           E I  EKE                +EEKS   P Q G++VL+ KEVEDG++ KEE     
Sbjct: 361 ERIEVEKEEVFGVVASEESTNNKNEEEKSHTDPIQVGVIVLEHKEVEDGQSSKEENHNST 420

Query: 421 ---AEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLEKR 465
              AE TEKVAAA     A A       AK RQG  GRKESPA+YN VIEETASKLLEKR
Sbjct: 421 EKEAEATEKVAAA-----AAAAAAEAAAAKPRQGGPGRKESPAVYNHVIEETASKLLEKR 468

BLAST of MC08g0348 vs. NCBI nr
Match: KAG6598809.1 (hypothetical protein SDJN03_08587, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 417 bits (1072), Expect = 6.68e-139
Identity = 303/502 (60.36%), Postives = 340/502 (67.73%), Query Frame = 0

Query: 1   MATSRAS----FGKERRITAPTSLTIPNY--STKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+ITAP+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKA 120
           SSSS       SSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   PAPIPEGK 
Sbjct: 61  SSSS-------SSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSGGPAPIPEGKP 120

Query: 121 SLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLER 180
           SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ +  SS K G LER
Sbjct: 121 SLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFLER 180

Query: 181 SSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSAL-AEIVPKAKNIAVEQA 240
           S S     KVGGK QQ VQ L+S SN   +KSL+RESSNA SA  AE+VPK KN AVE  
Sbjct: 181 SGSKIY--KVGGKTQQSVQTLKSSSNV--RKSLKRESSNAASASSAEMVPKTKN-AVEHV 240

Query: 241 VHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRT-DHVEKKVDVLDKEEADAKHE 300
             +P  VLG NEE+LKKIECELD  LPDP PELDKE+ T D VEK++             
Sbjct: 241 GQTPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH------------ 300

Query: 301 AEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEEKP 360
              QPIQDT+I      +K   ETP   E + AK  I+E+ NRE+ EG GEEE K+EEKP
Sbjct: 301 -HHQPIQDTKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKP 360

Query: 361 EIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKEE----- 420
           E I  EKE                +EEKS   P QEG++VL+ KEVEDG++ KEE     
Sbjct: 361 ERIEVEKEEVFGVVASEESTNNKNEEEKSHTDPIQEGVIVLEHKEVEDGQSSKEENHNST 420

Query: 421 ---AEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLEKR 465
              AE TEKVAAA     A AE  A   AK RQG  GRKESPA+YN VIEETASKLLEKR
Sbjct: 421 EKEAEATEKVAAA---ATAAAEAAA---AKPRQGGPGRKESPAVYNHVIEETASKLLEKR 467

BLAST of MC08g0348 vs. ExPASy TrEMBL
Match: A0A6J1BQC8 (uncharacterized protein DDB_G0271670 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004674 PE=4 SV=1)

HSP 1 Score: 809 bits (2090), Expect = 4.62e-293
Identity = 465/516 (90.12%), Postives = 465/516 (90.12%), Query Frame = 0

Query: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS 60
           MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS
Sbjct: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS 60

Query: 61  SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR 120
           SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR
Sbjct: 61  SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR 120

Query: 121 SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT 180
           SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT
Sbjct: 121 SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT 180

Query: 181 SPKV---------------------------------------------------GGKPQ 240
           SPKV                                                   GGKPQ
Sbjct: 181 SPKVEPSYLERSSSAKTSSKVEPSHLERSSSSKSSSKVEPSHLERSSSSKSSSKVGGKPQ 240

Query: 241 QPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELK 300
           QPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELK
Sbjct: 241 QPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELK 300

Query: 301 KIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTE 360
           KIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTE
Sbjct: 301 KIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTE 360

Query: 361 TPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEG 420
           TPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEG
Sbjct: 361 TPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEG 420

Query: 421 IVVLDQKEVEDGENCKEEAEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAMYN 465
           IVVLDQKEVEDGENCKEEAEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAMYN
Sbjct: 421 IVVLDQKEVEDGENCKEEAEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAMYN 480

BLAST of MC08g0348 vs. ExPASy TrEMBL
Match: A0A6J1BTH3 (uncharacterized protein DDB_G0271670 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004674 PE=4 SV=1)

HSP 1 Score: 796 bits (2056), Expect = 2.44e-287
Identity = 465/550 (84.55%), Postives = 465/550 (84.55%), Query Frame = 0

Query: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS 60
           MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS
Sbjct: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTS 60

Query: 61  SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR 120
           SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR
Sbjct: 61  SKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRR 120

Query: 121 SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT 180
           SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT
Sbjct: 121 SFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKT 180

Query: 181 SPKV-------------------------------------------------------- 240
           SPKV                                                        
Sbjct: 181 SPKVEPSYLERSSSAKTSSKVEPSHLERSSSSKSSSKVEPSHLERSSSAKTSSKVEPSHL 240

Query: 241 -----------------------------GGKPQQPVQNLRSGSNAIAKKSLRRESSNAG 300
                                        GGKPQQPVQNLRSGSNAIAKKSLRRESSNAG
Sbjct: 241 ERSSSSKSSSKVEPSHLERSSSSKSSSKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAG 300

Query: 301 SALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTDHV 360
           SALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTDHV
Sbjct: 301 SALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTDHV 360

Query: 361 EKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVG 420
           EKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVG
Sbjct: 361 EKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVG 420

Query: 421 EEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEVTEKVA 465
           EEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEVTEKVA
Sbjct: 421 EEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEVTEKVA 480

BLAST of MC08g0348 vs. ExPASy TrEMBL
Match: A0A6J1HE16 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462632 PE=4 SV=1)

HSP 1 Score: 412 bits (1060), Expect = 2.10e-137
Identity = 300/502 (59.76%), Postives = 339/502 (67.53%), Query Frame = 0

Query: 1   MATSRAS----FGKERRITAPTSLTIPNY--STKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+ITAP+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKA 120
           SSSS       SSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   PAPIPEGK 
Sbjct: 61  SSSS-------SSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSGGPAPIPEGKP 120

Query: 121 SLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLER 180
           SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ +  SS K G LER
Sbjct: 121 SLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFLER 180

Query: 181 SSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSAL-AEIVPKAKNIAVEQA 240
           S S     KVGGK QQ VQ L+S SN   +KSL+RESSNA SA  AE+VPK KN AVE  
Sbjct: 181 SGSKIY--KVGGKTQQSVQTLKSSSNV--RKSLKRESSNAASASSAEMVPKTKN-AVEHV 240

Query: 241 VHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRT-DHVEKKVDVLDKEEADAKHE 300
             SP  VLG NEE+LKKIECELD  LPDP PELDKE+ T D VEK++             
Sbjct: 241 GQSPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH------------ 300

Query: 301 AEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEEKP 360
              QPIQ+T+I      +K   ETP   E + AK  I+E+ NRE+ EG GEEE K+EEKP
Sbjct: 301 -HHQPIQETKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKP 360

Query: 361 EIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKEE----- 420
           E I  EKE                +EEKS   P QEG++VL+ KE EDG++ KEE     
Sbjct: 361 ERIEVEKEEVFGVVASEESTNNKNEEEKSHTVPIQEGVIVLEHKEAEDGQSSKEENHTST 420

Query: 421 ---AEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLEKR 465
               E TEKVAAA     + AE +A   AK RQG  GRKESPA+YN VIEETASKLLEKR
Sbjct: 421 EKETEATEKVAAA---ATSAAEAVA---AKPRQGGPGRKESPAVYNHVIEETASKLLEKR 467

BLAST of MC08g0348 vs. ExPASy TrEMBL
Match: A0A6J1K599 (microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492415 PE=4 SV=1)

HSP 1 Score: 410 bits (1055), Expect = 1.08e-136
Identity = 297/502 (59.16%), Postives = 332/502 (66.14%), Query Frame = 0

Query: 1   MATSRAS----FGKERRITAPTSLTIPN--YSTKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+IT P+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITTPSSVTIPNPNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKA 120
           S         SSSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   PAPIPEGK 
Sbjct: 61  S--------PSSSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSAGPAPIPEGKP 120

Query: 121 SLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLER 180
           SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ T  SS K G LER
Sbjct: 121 SLNRRRSFDKPPPAMQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTTLVSSTKTGFLER 180

Query: 181 SSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSAL-AEIVPKAKNIAVEQA 240
           S S     KVGGK QQ VQ  +S SN   +KSL+RESSNA SA  AE+VPK KN AVE  
Sbjct: 181 SGSKIY--KVGGKTQQSVQTFKSSSNL--RKSLKRESSNAASASSAEMVPKTKN-AVEHV 240

Query: 241 VHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRT-DHVEKKVDVLDKEEADAKHE 300
             SP  VLGVNEE+LKKIECELD  LPDP PELDKE+ T D VEK++             
Sbjct: 241 GQSPVLVLGVNEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH------------ 300

Query: 301 AEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEEKP 360
              QPIQDT+I      +K   ETP   E + AK  I E+ N+E+ EG GEEE K+EEKP
Sbjct: 301 -HHQPIQDTKIFKVVGSNKKVEETPGAAETDNAKSSIWEDENQEQNEGEGEEEAKEEEKP 360

Query: 361 EIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKEE----- 420
           E I  EKE                +EEKS   P QEG++VL+ KE EDG++ KEE     
Sbjct: 361 ERIEVEKEEVYGVVASEESTNNKNEEEKSHTDPIQEGVIVLEHKEAEDGQSSKEENHNST 420

Query: 421 ---AEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLEKR 465
              AE TEKVAAA           A   AK RQG  GRKESPA+YN VIEETASKLLEKR
Sbjct: 421 EKEAEATEKVAAAAA--------AAAEAAKPRQGGPGRKESPAVYNHVIEETASKLLEKR 464

BLAST of MC08g0348 vs. ExPASy TrEMBL
Match: A0A1S3BBU4 (serine/arginine repetitive matrix protein 1 OS=Cucumis melo OX=3656 GN=LOC103488229 PE=4 SV=1)

HSP 1 Score: 390 bits (1001), Expect = 1.01e-128
Identity = 288/490 (58.78%), Postives = 335/490 (68.37%), Query Frame = 0

Query: 1   MATSRAS----FGKERRITAPTSLTIPNYS-TKRTPKSSPRS--QQTDHKLAYSSSFSSS 60
           MAT+R+      GKERRIT P+S+TIPN + TKRTPKSSPR     TD K+    SFSSS
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVP---SFSSS 60

Query: 61  SSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGK 120
           S S S        S+  SLS+L EK VPNYLKSTASSR+DH      +    PAPIPEGK
Sbjct: 61  SPSPS--------SAPTSLSILPEKPVPNYLKSTASSRNDHNFKPITRSKSGPAPIPEGK 120

Query: 121 ASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPT---FPSSAKPG 180
            SLNRRRSFDKPPPTAPRL+KPFRSPGPR+RATHVPVRSSSFG+KP+ +      S KPG
Sbjct: 121 PSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTTIIGHSTKPG 180

Query: 181 HLERSSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSN-----AGSALAEIVPKA 240
            LERSSS  +  KVGGKPQ P+Q+L++ S+ + KKSLRRESSN     + SA AEIVPK 
Sbjct: 181 FLERSSSKIS--KVGGKPQPPIQSLKTSSSHV-KKSLRRESSNVAASASASASAEIVPKT 240

Query: 241 KNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKE 300
           KN AVE    SPSFVLGVNEE+LKKIECELDPYLPDPMPELDK++R D VEKK   LDK+
Sbjct: 241 KN-AVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQVRIDQVEKKA-ALDKD 300

Query: 301 EADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKP 360
           E D   E  K  + + E+   + ETP   E E  L     +NRE+ EG  EEE K+E+KP
Sbjct: 301 ETDT--EILKVVVTNKEVE--EKETPGTAETEISL-----ANREQNEGE-EEEPKEEDKP 360

Query: 361 EIITQEKEVDKDEEKSEISPTQEGIVVLDQKE------VEDGENCKEEAEVTEKVAAAE- 420
           + I  EKE        E+   +E I   + +E      V DGE+CKEE +      AAE 
Sbjct: 361 KRIGVEKE--------EVLALEESINNKNDQEKSETDSVLDGESCKEENDQNSTPKAAET 420

Query: 421 --KEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLEKRQNKVKALAGAFQ 465
             KE E+  EK A    K RQG+ GRKESPA+YN VIEETASKLLEKR+NKV+ALAGAFQ
Sbjct: 421 TEKEAEST-EKTA----KPRQGAPGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQ 451

BLAST of MC08g0348 vs. TAIR 10
Match: AT5G24880.1 (BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (TAIR:AT5G10660.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 74.3 bits (181), Expect = 2.8e-13
Identity = 153/480 (31.87%), Postives = 222/480 (46.25%), Query Frame = 0

Query: 26  STKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLK 85
           +T  T K+ P +Q        + S + S          T+SS   S     EK +PNYLK
Sbjct: 3   TTSSTTKAKPDNQ--------NLSRTKSLGRKPKQPLSTASSEDGSSDQKPEKPLPNYLK 62

Query: 86  STASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRA 145
            T SSR D G  K +KK        +    L RRRSFD+PP +   L  P  S  PR + 
Sbjct: 63  PTISSRPDAG--KLLKKNNNSL---DDNQKLLRRRSFDRPPSS---LTSPSTSASPRIQK 122

Query: 146 THVPVRSSSFGSKPAPTFPSSAKPGHLERSSS---SKTSPKVGGKPQQ-PVQNLRSGSNA 205
           +   +  S   S+  P  P   KP    RSSS   S+  PK G   +  PV   +SG N+
Sbjct: 123 S---LNVSPSRSRDRPAVPRE-KPVTALRSSSFHGSRNVPKGGNTAKSPPVAPKKSGLNS 182

Query: 206 IAKKSLRRESSNAGSALAEIVPKA-KNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLP 265
            +  S   +S   GS    I   + K IA++ A  S S     ++EE+ K+E        
Sbjct: 183 SSTSS---KSKKEGSENVRIKKASDKEIALDSA--SMSSAQEDHQEEILKVE-------S 242

Query: 266 DPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPI------ 325
           D +   D +I     EK     +++E   K     + +++   S   T   SP+      
Sbjct: 243 DHLQVSDHDIEEPKYEK-----EEKEVQEKVVQANESVEEKAESSGPTPVASPVGKDCNA 302

Query: 326 ---EVEAKLLIQEESNRERTEGVGEEE----------------VKDEEKPE-IITQEKEV 385
              E+E KL+  E+   E+TE + E++                + + E PE + T+ KEV
Sbjct: 303 VVAELEEKLIKNEDDIEEKTEEMKEQDNNQANKSEEEEDVKKKIDENETPEKVDTESKEV 362

Query: 386 DKDEE---KSEISPTQEGIVVLDQKE------VEDGENCKEEAEVTEKVAAAEKEGEAVA 445
           +  EE   + E    +EG   ++++E       ED +  K E E  EKV   ++E E V 
Sbjct: 363 ESVEETTQEKEEEVKEEGKERVEEEEKEKEKVKEDDQKEKVEEEEKEKV-KGDEEKEKVK 422

Query: 446 EKLAVMGAKQRQGSGRKESPAMYNDVIEETASKLLEK-RQNKVKALAGAFQTVIDYESSS 465
           E+ +  G K+    G+KESP+ YNDVI   ASK+ E  R+NKV ALAGAFQTVIDYE+++
Sbjct: 423 EEESAEGKKKEVVKGKKESPSAYNDVI---ASKMQENPRKNKVLALAGAFQTVIDYETAA 441

BLAST of MC08g0348 vs. TAIR 10
Match: AT5G10660.1 (calmodulin-binding protein-related )

HSP 1 Score: 60.8 bits (146), Expect = 3.2e-09
Identity = 139/451 (30.82%), Postives = 196/451 (43.46%), Query Frame = 0

Query: 30  TPKSSPRSQQTDHKLAYSSSFSSSSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTAS 89
           T  S+ ++++ +  L  S S        S+S    ++  S   ++   K +PNYLK T S
Sbjct: 3   TTSSTTKTKRDNKNLTRSKSLGRKPKPVSSSEPEANADGSDRKTI--GKPLPNYLKPTIS 62

Query: 90  SRHDHGSFKTVKKFVAPAPIPEGKASLNRRRSFDKPPP--TAPRLDKPFRSPGPRSRATH 149
           SR D        KF+      E    L RRRSFD PP   T+P      RS        H
Sbjct: 63  SRPD------PVKFLRKNNAVEENQKLLRRRSFDHPPSSLTSPSTSSAHRSLNTSPAHPH 122

Query: 150 VPVRSSSFGSKPAPTFPSSAKPGHLE---RSSSSKTSPKVGGKPQQPVQNLRSGSNAIAK 209
           +  + +    KP     S++  G      R SS+  SP V  +    V+      N+ + 
Sbjct: 123 LRDKPAVPREKPVTGLRSTSFHGSSRGGLRGSSTVKSPPVASRGSSGVKKSGLSGNS-SS 182

Query: 210 KSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMP 269
           KS +  S N        VPK  +        SP      +EEE+ K+E ++         
Sbjct: 183 KSKKEGSGN--------VPKKSSGKEISPDSSPLASAHEDEEEIVKVETDVH-------- 242

Query: 270 ELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQE 329
                  +DH E+      KEE   +     +  ++ E S     T    E +   LI E
Sbjct: 243 ------ISDHGEE-----PKEEDKDQFAQPDESGEEKETSPVAAST----EEQKGELIDE 302

Query: 330 ESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPT----QEGIVVLDQKEVED 389
           + + E+ E   E E  +E   E   + K+   DEE SE   T     E + V + KE E 
Sbjct: 303 DKSTEQIEEPKEPENIEENNSEEEEEVKKKSDDEENSETVATTTDMNEAVNVEESKEEE- 362

Query: 390 GENCKEEAEVTEK----VAAAEKEGEAVA--EKLAVMGAKQRQGSGRKESPAMYNDVIEE 449
               KEEAEV E+     AA E+  E +A  E+L   G K     G+KESP  YNDVI  
Sbjct: 363 ----KEEAEVKEEEGESSAAKEETTETMAQVEELPEEGTKNEVVQGKKESPTAYNDVI-- 405

Query: 450 TASKLLE-KRQNKVKALAGAFQTVIDYESSS 465
            ASK+ E  ++NKV ALAGAFQTVIDYE+++
Sbjct: 423 -ASKMQENSKKNKVLALAGAFQTVIDYETAA 405