MC08g0345 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0345
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Aminoacyl-tRNA hydrolase
Location	MC08: 2699055 .. 2700576 (+)
RNA-Seq Expression	MC08g0345
Synteny	MC08g0345

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCTCATATTTATTATTTGGAAAACAATGCCAGCTAAGCTTCATTATTTAATTATATTCAATTAAACAAATCTTATTGTACAATATCATAAAATCCTAAAAAAAAAAAAAAAGAAGCTAAAATATGGTGGGCCCAACCTTCAGGCCCAAAAGGCCCATTAGAAATGCTGGACGGACGGCTTCCCTTGAAATCTCAAGGTTCGGTAGCACGGTTTCACATGCCTTTGTGGGTAACGATATCATGACATCTGTAGTTTCAGCATTCTCATTCTCCATCCCCAATTTCTGCAGGCTCTGTTCAAGGATTAGGGCTTCGCCATCTTCTTCACTCCCTCTTCGCTATCGGAACGGAAATTCCAGGTTATTTAGCTCGGTCTCGATGAGTGAATCCGTTGAAAGTAATTCCGTTCCTCCAACGGACGACGGCGGAGCGAAGGCGGAGGAACGAGCCAAGGAGGAGACCGCCGATGTTTTGGTTCAGTACGTGGTGCTCCGGAGAGATCTGATCGACTCGTGGCCGCTCGGAAGCGTCGTAACTCAAGGATGCCATGCTTCAGTCTCCGCCATTTGGTTGAACAAGGACGATTCCCACACTTCTGAGTACTGTAGTCCTGGTAACATCGATTCTATGCACAAGGTAAGTCAAGCTTTTAACTCCATTTTTGTTTTTGATTTTCAAGTAAAGCACCTCGAACAATGTAGTATGGGTTCGTGGCTACTATCTTTGTATGTTGATGTGCCGAATATTTGGTTTCTCGGTGCTCTATTTAGTCGGTCTGACAGTTGAGATATTCTGAGCAATTTGGAAGTTTTATGCTGTGGTCGCTGGTTGATTTATTCAGTGCTGGGAAGTACATTTTATTTGTAGTTTTCGATCTTTCTTCCGTCAGGTGTTTGAACATTTCGGAAGAACAATGGTTGATTGGAAAGCCTATTCTTATTTCTGTTCGCGTTGTTTTCCATGTGAAGAATTATTAAACTGTGTTCTGATTTTCCACCTTAATTCCACAATTGTATCCTTCCAGTATCTACATTATAATCGGAGGTCAAGGAGAGCTCATTTATCTCATATTTCTGCTTGTACATTTTCTTTTAGTTTTATGTGGTCTTCAATCATTTTCATGGTTAAAATGCATTCCTCTTCATTCGCATTTTTGTTAAACGATTGAACTGCCAAACCTCTTGTTTATTGGCAAATTCCTTGCGTATCAGATTAATATCTTGTGCAACTTTGTTGAATTAACTGGCATACTTCCGTTGATCTATTAAGCCATACTGGACTTTTTGATGCCTGAAGGTGGGTTTTAAGAAGCTGATTGTATTTCACTTTTTCCATATGTAGATTACTGTCGAGGTGAAAGGAGAAACCCAGATCGTTAACTTGTCAGAGAAACTCAAAGCAAATTCAATCGCCCATAAACTGTGGATCGAACAACCCGAGAACATTCCAACATGCCTTGCCACAAAGCCCTACCCCAAATCTCTAGTATCAGCATTTTTTAAAAAGCTGAAACTCTGTAAG

mRNA sequence

GTTCTCATATTTATTATTTGGAAAACAATGCCAGCTAAGCTTCATTATTTAATTATATTCAATTAAACAAATCTTATTGTACAATATCATAAAATCCTAAAAAAAAAAAAAAAGAAGCTAAAATATGGTGGGCCCAACCTTCAGGCCCAAAAGGCCCATTAGAAATGCTGGACGGACGGCTTCCCTTGAAATCTCAAGGTTCGGTAGCACGGTTTCACATGCCTTTGTGGGTAACGATATCATGACATCTGTAGTTTCAGCATTCTCATTCTCCATCCCCAATTTCTGCAGGCTCTGTTCAAGGATTAGGGCTTCGCCATCTTCTTCACTCCCTCTTCGCTATCGGAACGGAAATTCCAGGTTATTTAGCTCGGTCTCGATGAGTGAATCCGTTGAAAGTAATTCCGTTCCTCCAACGGACGACGGCGGAGCGAAGGCGGAGGAACGAGCCAAGGAGGAGACCGCCGATGTTTTGGTTCAGTACGTGGTGCTCCGGAGAGATCTGATCGACTCGTGGCCGCTCGGAAGCGTCGTAACTCAAGGATGCCATGCTTCAGTCTCCGCCATTTGGTTGAACAAGGACGATTCCCACACTTCTGAGTACTGTAGTCCTGGTAACATCGATTCTATGCACAAGATTACTGTCGAGGTGAAAGGAGAAACCCAGATCGTTAACTTGTCAGAGAAACTCAAAGCAAATTCAATCGCCCATAAACTGTGGATCGAACAACCCGAGAACATTCCAACATGCCTTGCCACAAAGCCCTACCCCAAATCTCTAGTATCAGCATTTTTTAAAAAGCTGAAACTCTGTAAG

Coding sequence (CDS)

ATGGTGGGCCCAACCTTCAGGCCCAAAAGGCCCATTAGAAATGCTGGACGGACGGCTTCCCTTGAAATCTCAAGGTTCGGTAGCACGGTTTCACATGCCTTTGTGGGTAACGATATCATGACATCTGTAGTTTCAGCATTCTCATTCTCCATCCCCAATTTCTGCAGGCTCTGTTCAAGGATTAGGGCTTCGCCATCTTCTTCACTCCCTCTTCGCTATCGGAACGGAAATTCCAGGTTATTTAGCTCGGTCTCGATGAGTGAATCCGTTGAAAGTAATTCCGTTCCTCCAACGGACGACGGCGGAGCGAAGGCGGAGGAACGAGCCAAGGAGGAGACCGCCGATGTTTTGGTTCAGTACGTGGTGCTCCGGAGAGATCTGATCGACTCGTGGCCGCTCGGAAGCGTCGTAACTCAAGGATGCCATGCTTCAGTCTCCGCCATTTGGTTGAACAAGGACGATTCCCACACTTCTGAGTACTGTAGTCCTGGTAACATCGATTCTATGCACAAGATTACTGTCGAGGTGAAAGGAGAAACCCAGATCGTTAACTTGTCAGAGAAACTCAAAGCAAATTCAATCGCCCATAAACTGTGGATCGAACAACCCGAGAACATTCCAACATGCCTTGCCACAAAGCCCTACCCCAAATCTCTAGTATCAGCATTTTTTAAAAAGCTGAAACTCTGTAAG

Protein sequence

MVGPTFRPKRPIRNAGRTASLEISRFGSTVSHAFVGNDIMTSVVSAFSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK

Homology

[Close]

BLAST of MC08g0345 vs. ExPASy Swiss-Prot
Match: Q3SZ85 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-26
Identity = 58/119 (48.74%), Postives = 81/119 (68.07%), Query Frame = 0


Query: 116 VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKI 175
           +LVQY+VLR+DL     SWP G+VV Q CHA+ +A+ L++D  HT+ Y     ++ M K+
Sbjct: 24  ILVQYLVLRKDLTQPPFSWPAGAVVAQACHAATAALHLHRDHPHTAAYLR--ELERMRKV 83

Query: 176 TVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 232
            +E   ET +  L+E L+  +I HKLW+EQPENIPTC+A +PYPK  VS + KK +L K
Sbjct: 84  VLEAPDETTLKLLAETLQQKNIDHKLWMEQPENIPTCIALRPYPKEEVSQYLKKFRLFK 140

[Close]

BLAST of MC08g0345 vs. ExPASy Swiss-Prot
Match: D3Z4S3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-24
Identity = 55/119 (46.22%), Postives = 78/119 (65.55%), Query Frame = 0


Query: 116 VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKI 175
           +LVQY+VLR+DL     SWP G++V Q CHA+ +A+ L++D  HT+ Y     +  M K+
Sbjct: 24  ILVQYLVLRKDLSQAPFSWPTGALVAQACHAATAALHLHRDHPHTAAYLR--ELGRMRKV 83

Query: 176 TVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 232
            +E   ET +  L+E L+  +I H LW+EQPENI TC+A +PYPK  VS + KK +L K
Sbjct: 84  VLEAADETTLKELAETLQQKNIDHTLWLEQPENIATCIALRPYPKEEVSQYLKKFRLFK 140

[Close]

BLAST of MC08g0345 vs. ExPASy Swiss-Prot
Match: Q6GMV3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.5e-23
Identity = 54/119 (45.38%), Postives = 76/119 (63.87%), Query Frame = 0


Query: 116 VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKI 175
           VLVQY+VLR+DL     SWP G++V Q CHA+ +A+  ++D  HT+ Y     +  M K+
Sbjct: 24  VLVQYLVLRKDLSQAPFSWPAGALVAQACHAATAALHTHRDHPHTAAYLQ--ELGRMRKV 83

Query: 176 TVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 232
            +E   ET +  L+E L+  +I H LW+EQPENI TC+A +PYPK  V  + KK +L K
Sbjct: 84  VLEAPDETTLKELAETLQQKNIDHMLWLEQPENIATCIALRPYPKEEVGQYLKKFRLFK 140

[Close]

BLAST of MC08g0345 vs. NCBI nr
Match: XP_022132277.1 (uncharacterized protein LOC111005172 [Momordica charantia])

HSP 1 Score: 459 bits (1180), Expect = 9.31e-163
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0


Query: 1   MVGPTFRPKRPIRNAGRTASLEISRFGSTVSHAFVGNDIMTSVVSAFSFSIPNFCRLCSR 60
           MVGPTFRPKRPIRNAGRTASLEISRFGSTVSHAFVGNDIMTSVVSAFSFSIPNFCRLCSR
Sbjct: 1   MVGPTFRPKRPIRNAGRTASLEISRFGSTVSHAFVGNDIMTSVVSAFSFSIPNFCRLCSR 60

Query: 61  IRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTDDGGAKAEERAKEETADVLVQY 120
           IRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTDDGGAKAEERAKEETADVLVQY
Sbjct: 61  IRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTDDGGAKAEERAKEETADVLVQY 120

Query: 121 VVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKITVEVKGET 180
           VVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKITVEVKGET
Sbjct: 121 VVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKITVEVKGET 180

Query: 181 QIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 231
           QIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK
Sbjct: 181 QIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 231

[Close]

BLAST of MC08g0345 vs. NCBI nr
Match: XP_023546189.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 299 bits (765), Expect = 5.06e-100
Identity = 160/206 (77.67%), Postives = 171/206 (83.01%), Query Frame = 0


Query: 30  VSHAFVGNDIMTSVVSA--FSFSIPNFCRLCS--RIRASPSSSLPLRYRNGNSRLFSSVS 89
           VS A +  DIM SV+SA  F+FSI    +LCS  R R  PSSSL LR RN NS LFSS S
Sbjct: 2   VSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSAS 61

Query: 90  MSESVESNSVPPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASV 149
           MSES ESNS PP DDG         E+TADVL+QYVVLRRDLIDSWPLGSVVTQGCHASV
Sbjct: 62  MSESAESNSAPPPDDG--------VEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASV 121

Query: 150 SAIWLNKDDSHTSEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPEN 209
           SA+WLNKDD HTSEYCSP NIDSMHK+T+EVKGETQIVNLSEKLKA+SIAHKLWIEQPEN
Sbjct: 122 SAVWLNKDDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEN 181

Query: 210 IPTCLATKPYPKSLVSAFFKKLKLCK 231
           IPTCLATKPYPKS+VSAFFKKLKLCK
Sbjct: 182 IPTCLATKPYPKSVVSAFFKKLKLCK 199

[Close]

BLAST of MC08g0345 vs. NCBI nr
Match: XP_038884124.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Benincasa hispida])

HSP 1 Score: 294 bits (753), Expect = 2.57e-98
Identity = 156/196 (79.59%), Postives = 165/196 (84.18%), Query Frame = 0


Query: 40  MTSVVSA--FSFSIPNFCRLCS--RIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 99
           MT V+SA  F+FSI    RLCS    R SPSSSL LR  N NS  FSS SMS+S E+NSV
Sbjct: 1   MTCVISASRFAFSISKSTRLCSCSSPRVSPSSSLSLRSWNRNSNPFSSASMSQSAETNSV 60

Query: 100 PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 159
           PP DDGGAKAE     E ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD 
Sbjct: 61  PPPDDGGAKAE-----EIADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDP 120

Query: 160 HTSEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 219
           HTS+YCSP NIDSMHK+T+EVKGETQIVNLSEKLKANSI HKLWIEQPENIPTCLATKPY
Sbjct: 121 HTSDYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWIEQPENIPTCLATKPY 180

Query: 220 PKSLVSAFFKKLKLCK 231
           PKS+VS FFKKLKLCK
Sbjct: 181 PKSIVSPFFKKLKLCK 191

[Close]

BLAST of MC08g0345 vs. NCBI nr
Match: XP_022962062.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita moschata])

HSP 1 Score: 288 bits (737), Expect = 6.31e-96
Identity = 153/196 (78.06%), Postives = 164/196 (83.67%), Query Frame = 0


Query: 40  MTSVVSA--FSFSIPNFCRLCS--RIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 99
           M SV+SA  F+FSI    +LCS  R R  PSSSL LR RN NS LFSS SMSES ESNS 
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSA 60

Query: 100 PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 159
           PP DD        + E+TADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNKDD 
Sbjct: 61  PPPDD--------SVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDP 120

Query: 160 HTSEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 219
           HT EYCSP NIDSMHK+T+EVKGETQIVNLSEKL+ANSIAHKLWIEQPENIPTCLATKPY
Sbjct: 121 HTFEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLATKPY 180

Query: 220 PKSLVSAFFKKLKLCK 231
           PKS+VSAFFKKLKLCK
Sbjct: 181 PKSVVSAFFKKLKLCK 188

[Close]

BLAST of MC08g0345 vs. NCBI nr
Match: XP_023002512.1 (putative peptidyl-tRNA hydrolase PTRHD1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 288 bits (736), Expect = 9.27e-96
Identity = 153/194 (78.87%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 40  MTSVVSA--FSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPP 99
           M SV+SA  F+FSI N  RLCS  R    SS+ L   N NS  FSS SMSES ESNSV P
Sbjct: 1   MASVISASRFTFSISNSARLCSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSVLP 60

Query: 100 TDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHT 159
            DDGGAKAE     E ADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD HT
Sbjct: 61  LDDGGAKAE-----EAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHT 120

Query: 160 SEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPK 219
           SEYCSP NIDSMHK+T+EVKGETQIVNLSEKLKANSI HKLW+EQPENIPTCLA+KPYPK
Sbjct: 121 SEYCSPQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPK 180

Query: 220 SLVSAFFKKLKLCK 231
           S+VS FFKKLKLCK
Sbjct: 181 SVVSPFFKKLKLCK 189

[Close]

BLAST of MC08g0345 vs. ExPASy TrEMBL
Match: A0A6J1BTE6 (Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111005172 PE=4 SV=1)

HSP 1 Score: 459 bits (1180), Expect = 4.51e-163
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0


Query: 1   MVGPTFRPKRPIRNAGRTASLEISRFGSTVSHAFVGNDIMTSVVSAFSFSIPNFCRLCSR 60
           MVGPTFRPKRPIRNAGRTASLEISRFGSTVSHAFVGNDIMTSVVSAFSFSIPNFCRLCSR
Sbjct: 1   MVGPTFRPKRPIRNAGRTASLEISRFGSTVSHAFVGNDIMTSVVSAFSFSIPNFCRLCSR 60

Query: 61  IRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTDDGGAKAEERAKEETADVLVQY 120
           IRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTDDGGAKAEERAKEETADVLVQY
Sbjct: 61  IRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTDDGGAKAEERAKEETADVLVQY 120

Query: 121 VVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKITVEVKGET 180
           VVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKITVEVKGET
Sbjct: 121 VVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKITVEVKGET 180

Query: 181 QIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 231
           QIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK
Sbjct: 181 QIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 231

[Close]

BLAST of MC08g0345 vs. ExPASy TrEMBL
Match: A0A6J1HBR1 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1)

HSP 1 Score: 288 bits (737), Expect = 3.06e-96
Identity = 153/196 (78.06%), Postives = 164/196 (83.67%), Query Frame = 0


Query: 40  MTSVVSA--FSFSIPNFCRLCS--RIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 99
           M SV+SA  F+FSI    +LCS  R R  PSSSL LR RN NS LFSS SMSES ESNS 
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSA 60

Query: 100 PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 159
           PP DD        + E+TADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNKDD 
Sbjct: 61  PPPDD--------SVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDP 120

Query: 160 HTSEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 219
           HT EYCSP NIDSMHK+T+EVKGETQIVNLSEKL+ANSIAHKLWIEQPENIPTCLATKPY
Sbjct: 121 HTFEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLATKPY 180

Query: 220 PKSLVSAFFKKLKLCK 231
           PKS+VSAFFKKLKLCK
Sbjct: 181 PKSVVSAFFKKLKLCK 188

[Close]

BLAST of MC08g0345 vs. ExPASy TrEMBL
Match: A0A6J1KJQ6 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1)

HSP 1 Score: 288 bits (736), Expect = 4.49e-96
Identity = 153/194 (78.87%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 40  MTSVVSA--FSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPP 99
           M SV+SA  F+FSI N  RLCS  R    SS+ L   N NS  FSS SMSES ESNSV P
Sbjct: 1   MASVISASRFTFSISNSARLCSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSVLP 60

Query: 100 TDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHT 159
            DDGGAKAE     E ADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD HT
Sbjct: 61  LDDGGAKAE-----EAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHT 120

Query: 160 SEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPK 219
           SEYCSP NIDSMHK+T+EVKGETQIVNLSEKLKANSI HKLW+EQPENIPTCLA+KPYPK
Sbjct: 121 SEYCSPQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPK 180

Query: 220 SLVSAFFKKLKLCK 231
           S+VS FFKKLKLCK
Sbjct: 181 SVVSPFFKKLKLCK 189

[Close]

BLAST of MC08g0345 vs. ExPASy TrEMBL
Match: A0A6J1GJ11 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454312 PE=4 SV=1)

HSP 1 Score: 286 bits (732), Expect = 1.82e-95
Identity = 153/194 (78.87%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 40  MTSVVSA--FSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPP 99
           M SV+SA  F+FSI N  RLC   R    SS+ LR  N NS  FSS SMSES ESNSV P
Sbjct: 1   MASVISASRFTFSISNSLRLCWNARVWLPSSICLRSWNWNSISFSSASMSESGESNSVLP 60

Query: 100 TDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHT 159
            DDGGAKAE     E ADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD HT
Sbjct: 61  LDDGGAKAE-----EAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHT 120

Query: 160 SEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPK 219
           SEYCSP NIDSMHK+T+EVKGETQIVNLSEKLKANSI HKLW+EQPENIPTCLA+KPYPK
Sbjct: 121 SEYCSPQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPK 180

Query: 220 SLVSAFFKKLKLCK 231
           S+VS FFKKLKLCK
Sbjct: 181 SVVSPFFKKLKLCK 189

[Close]

BLAST of MC08g0345 vs. ExPASy TrEMBL
Match: A0A6J1K304 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491924 PE=4 SV=1)

HSP 1 Score: 285 bits (730), Expect = 3.55e-95
Identity = 153/196 (78.06%), Postives = 161/196 (82.14%), Query Frame = 0


Query: 40  MTSVVSA--FSFSIPNFCRLCS--RIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 99
           M SV+SA  F+FSI    +LCS  R R  PSSSL LR RN NS LFSS SMSES ESNS 
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSA 60

Query: 100 PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 159
           PP DD          E+ ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD 
Sbjct: 61  PPPDD--------IVEKAADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDP 120

Query: 160 HTSEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 219
            TSEYCSP NIDSMHK+T+EVKGETQIVNLSEKLK NSIAHKLWIEQPENIPTCLATKPY
Sbjct: 121 QTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPY 180

Query: 220 PKSLVSAFFKKLKLCK 231
           PKS+VSAFFKKLKLCK
Sbjct: 181 PKSVVSAFFKKLKLCK 188

[Close]

BLAST of MC08g0345 vs. TAIR 10
Match: AT5G10700.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 201.8 bits (512), Expect = 5.9e-52
Identity = 112/196 (57.14%), Postives = 137/196 (69.90%), Query Frame = 0


Query: 40  MTSVVSAFSFSIPNFCRLCS----RIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 99
           M + V + +F  P   R  S    RIR   S+      R  + R F++ S S S ++  +
Sbjct: 1   MATAVLSAAFRFPIVTRRLSAPAPRIRFYHST-----IRFAHFRRFTTASPSMSQQTGEI 60

Query: 100 PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 159
                  A  +  + E+  DV+VQYVVLRRDLIDSWPLGSVVTQGCHASV+AIW  KDD 
Sbjct: 61  ------DAVVDASSAEKPDDVVVQYVVLRRDLIDSWPLGSVVTQGCHASVAAIWSFKDDP 120

Query: 160 HTSEYCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 219
            T +YC P +IDSMHK+T+EVKGETQ++NLSEKLK   I+HKLW+EQPENIPTC+ATKPY
Sbjct: 121 VTLQYCDPQHIDSMHKVTLEVKGETQMMNLSEKLKLGGISHKLWMEQPENIPTCIATKPY 180

Query: 220 PKSLVSAFFKKLKLCK 232
           PKS VS+FFKKLKLCK
Sbjct: 181 PKSQVSSFFKKLKLCK 185

The following BLAST results are available for this feature:

BLAST of MC08g0345 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3SZ85	2.4e-26	48.74	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=...	[more]
D3Z4S3	2.3e-24	46.22	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 ...	[more]
Q6GMV3	1.5e-23	45.38	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 S...	[more]

BLAST of MC08g0345 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132277.1	9.31e-163	100.00	uncharacterized protein LOC111005172 [Momordica charantia]	[more]
XP_023546189.1	5.06e-100	77.67	putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo]	[more]
XP_038884124.1	2.57e-98	79.59	putative peptidyl-tRNA hydrolase PTRHD1 [Benincasa hispida]	[more]
XP_022962062.1	6.31e-96	78.06	putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita moschata]	[more]
XP_023002512.1	9.27e-96	78.87	putative peptidyl-tRNA hydrolase PTRHD1 isoform X1 [Cucurbita maxima]	[more]

BLAST of MC08g0345 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTE6	4.51e-163	100.00	Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111005172 PE=4 SV=...	[more]
A0A6J1HBR1	3.06e-96	78.06	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1	[more]
A0A6J1KJQ6	4.49e-96	78.87	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1	[more]
A0A6J1GJ11	1.82e-95	78.87	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454312 PE=4 SV=1	[more]
A0A6J1K304	3.55e-95	78.06	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491924 PE=4 SV=1	[more]

BLAST of MC08g0345 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10700.1	5.9e-52	57.14	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PFAM	PF01981	PTH2	coord: 118..229 e-value: 1.2E-23 score: 83.6
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	GENE3D	3.40.1490.10	Bit1	coord: 117..230 e-value: 1.2E-17 score: 66.0
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 117..230
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 88..109
None	No IPR available	CDD	cd02429	PTH2_like	coord: 117..230 e-value: 3.30138E-66 score: 198.397
IPR042237	Putative peptidyl-tRNA hydrolase PTRHD1	PANTHER	PTHR46194	PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED	coord: 86..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0345.1	MC08g0345.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity