MC08g0314 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g0314 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 4.8e-156
Identity = 286/570 (50.18%), Postives = 400/570 (70.18%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME P    E + ++    K +  KGG+ T+PFIIAN A EKVAS GL P+MI+YL R Y 
Sbjct: 1   MENPPNETEAKQIQTNEGKKT--KGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYR 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
              A  + V+F+WSAASNFTP++ AFL+DSYLGRF  I+  S+ SFLGM +LWLTA +PQ
Sbjct: 61  FGVAKGTNVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQ 120

Query: 128 ARPF-CDELS--GHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQL-----LKS 187
            +P  CD  +   HC + +  QL LLYS++AL++IGSG ++  S+AFGADQL      K+
Sbjct: 121 VKPSPCDPTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKN 180

Query: 188 SGTLKNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLY 247
              L+++F   Y + +  VL+  + IVYIQ+H+GW +GFGVP   ML +++ F+ ASPLY
Sbjct: 181 ERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLY 240

Query: 248 VKSMPSSSLCTGLVQVVVASYKKRRMKVPS-TDTYEIYHHNNGSPHLMPSDKLRFLNKAC 307
           V    + SL TGL Q +VA+YKKR++ +P   D+++ Y+H   S    PS KLRFLNKAC
Sbjct: 241 VTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKAC 300

Query: 308 IISNSEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQL 367
           +ISN EEE+ SDG A NPW LCT D+VE LKALI+++P+WSTGI++++  +Q SF +LQ 
Sbjct: 301 LISNREEEIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQA 360

Query: 368 ASMDRHLT---PTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRM 427
            SMDR L+    +F+VPAGSFG+  I++L +W+ILYDR ++PL SKIRG+  RLS K RM
Sbjct: 361 TSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRM 420

Query: 428 GIGILFCTFSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSA 487
           G+G+     ++  +A+VE+ RR  AI +G++++  AVV +SA W +P+Y+  G++E  +A
Sbjct: 421 GLGLFMSFLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTA 480

Query: 488 VGQIEFFYFELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINN 547
           +GQ EFFY E P++MSS+A SLFGLGM+V +L AS ++  V+  +   G ESWVS NIN 
Sbjct: 481 IGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINK 540

Query: 548 GHCDYYYWLLFALVFANFLYFLVCSKSYGP 565
           GH +YYYW+L  + F N +Y+++CS SYGP
Sbjct: 541 GHYNYYYWVLAIMSFINVIYYVICSWSYGP 568

BLAST of MC08g0314 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 534.3 bits (1375), Expect = 1.8e-150
Identity = 284/576 (49.31%), Postives = 389/576 (67.53%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME P    E ++  +  +     KGGL T+PFIIAN   EKVAS GL  +MILYL   Y 
Sbjct: 1   MENPPDQTESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYR 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           +      TV+F+W AA+NF P++ AFL+DSYLGRF  I   S+ S LGM VLWLTA +PQ
Sbjct: 61  LGLVKGQTVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQ 120

Query: 128 ARPF-CDELSG-HCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQL-----LKSS 187
            +P  C   +G +C++ ++ QL LLY+++AL++IGSG ++  S+AFGADQL      K+ 
Sbjct: 121 VKPSPCVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNE 180

Query: 188 GTLKNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYV 247
             L+++F   Y + S  VL+  + IVYIQDH+GW +GFG+P   ML +   F+ ASPLYV
Sbjct: 181 RVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYV 240

Query: 248 KSMPSSSLCTGLVQVVVASYKKRRMKVPS-TDTYEIYHHNNGSPHLMPSDKLRFLNKACI 307
           K   S SL TGL QVV A+Y KR + +P   D+ + Y+    S    PSDKLRFLNKAC 
Sbjct: 241 KRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACA 300

Query: 308 ISNSEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQLA 367
           ISN +E+L SDG A N W LCT DQVE LKAL++++P+WSTGI++++ V+Q SF +LQ  
Sbjct: 301 ISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAK 360

Query: 368 SMDRHLT--PTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGI 427
           SMDR L+   TF++PAGSFG+  I++LI W++LYDR ILPL SKIRG+  R++ K RMG+
Sbjct: 361 SMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGL 420

Query: 428 GILFCTFSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVG 487
           G+     ++  +A VE  RR  AI +G ++D  + VS+SA W +P+Y+  G++E  + +G
Sbjct: 421 GLFISFLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIG 480

Query: 488 QIEFFYFELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGH 547
           Q EFFY E P++MSS+A SLFGLGM+V N+ AS I+  V N SK  G  SW+  NIN GH
Sbjct: 481 QTEFFYTEFPKSMSSIAASLFGLGMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGH 540

Query: 548 CDYYYWLLFALVFANFLYFLVCSKSYGPSKEEASDE 573
            DYYYW+L  L F N +Y++VCS SYGP+ ++  ++
Sbjct: 541 YDYYYWVLAILSFVNVIYYVVCSWSYGPTVDQVRND 575

BLAST of MC08g0314 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.5e-96
Identity = 200/565 (35.40%), Postives = 329/565 (58.23%), Query Frame = 0

Query: 32  GGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIFLWSAASNFTPIIC 91
           GG R + FI+ N  LE++ S GL  + ++YLT+V+ +E   A+ VI +WS  +N TP++ 
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 92  AFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFC----DELSGHCNAPSTPQL 151
           A+++D+Y+GRF  IA  S  + LG+  + LTA+ PQ  P      D LS  C  P+  Q+
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLS--CGGPNKLQI 172

Query: 152 LLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGT----LKNYFSLVYMAQSAGVLVGMS 211
            +L      +++GSG ++  SI FG DQ  + +      + ++F+  YM  +  +++  +
Sbjct: 173 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQT 232

Query: 212 CIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGLVQVVVASYKKR 271
            +VYIQD V W +GF +P   M  + + F +    YV   P  S+ +G+ QV+VA+ KKR
Sbjct: 233 VVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKR 292

Query: 272 RMKVPSTDTYEIYHH-----NNGSPHLMPSDKLRFLNKACIISNSEEELTSDGRASNPWS 331
           ++K+P+ D   + ++     ++    L  S++ R L+KA ++   E +LT +G  ++ W 
Sbjct: 293 KLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWR 352

Query: 332 LCTVDQVEGLKALIRIVPLWSTGI--LVAVGVNQSFYVLQLASMDRHLTPTFEVPAGSFG 391
           LC+V +VE +K LIRIVP+WS GI  L A+    +F V Q   MDR+L P FE+PAGS  
Sbjct: 353 LCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLS 412

Query: 392 VVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVATAIVETNRR 451
           V+ ++++ I++  YDR+ +P + +I G ++ ++   R+G GI+F  FS++   IVE  RR
Sbjct: 413 VISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRR 472

Query: 452 AMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMSSVATSL 511
             +I  G   DP  +  MS FW  P+ I +G+ E F+ +GQIEFF  + P  M S+A SL
Sbjct: 473 IRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSL 532

Query: 512 FGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVFANFLYFL 571
           F L  +  +  +SF++T+V  FS       W++ N+N G  DY+Y+L+  L   N +YF 
Sbjct: 533 FSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFW 592

Query: 572 VCSKSY----GPSKEEASDESNAED 578
            C++ Y    G   E+  ++ +++D
Sbjct: 593 YCARGYRYKVGLPIEDFEEDKSSDD 610

BLAST of MC08g0314 vs. ExPASy Swiss-Prot
Match: Q9LYD5 (Protein NRT1/ PTR FAMILY 1.3 OS=Arabidopsis thaliana OX=3702 GN=NPF1.3 PE=2 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 6.8e-94
Identity = 218/554 (39.35%), Postives = 305/554 (55.05%), Query Frame = 0

Query: 19  DMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIF 78
           D E  L+  +  K G+ T+PFI+A+ ALEK+A  GL P+MIL+LT  YGM +A A+ ++F
Sbjct: 2   DQEALLVGRTLLKRGIPTIPFILASQALEKLAYFGLVPNMILFLTVEYGMGTAEAANILF 61

Query: 79  LWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCDELSGH 138
           LWSAA+NF P++ AF+ADSY GRFP+I  GS +S  GM +LWLT  I   RP CD+L+  
Sbjct: 62  LWSAATNFFPLVGAFIADSYTGRFPLIGFGSSISLTGMVLLWLTTII---RPECDKLTNV 121

Query: 139 CNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK------SSGTLKNYFSLVYM 198
           C   +  + +LLYS +AL AIG+G ++SS +AF ADQL        ++ +L+  F+  Y 
Sbjct: 122 CQPTTLLKSVLLYSFFALTAIGAGGVRSSCLAFAADQLQPNQTSRVTTSSLETLFNWYYF 181

Query: 199 AQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGL 258
           +      +  S +V++Q   GW +GFGV VA M  S   F +ASP YV            
Sbjct: 182 SVMVACFLSQSLLVFVQTTYGWQIGFGVSVAAMALSVALFFAASPYYV------------ 241

Query: 259 VQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEELTSDGR 318
                      R + P+ +                                         
Sbjct: 242 -----------RFQKPTRN----------------------------------------- 301

Query: 319 ASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAV--GVNQSFYVLQLASMDRH-LTPTFE 378
           + NPW LC V QVE LK+LI ++P+WSTGI++++      SF VLQ  +MDRH     FE
Sbjct: 302 SRNPWKLCRVQQVEDLKSLINVIPIWSTGIILSLVTACQVSFIVLQAKTMDRHTFIQGFE 361

Query: 379 VPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVATA 438
           +P GS+G+ L++S ++++ LYD +I+PL+S    +  RL    RM  G +     + A A
Sbjct: 362 IPPGSYGIFLVISFLLFLGLYDLVIVPLLSWALREPFRLGVMVRMWAGYVISVLCISALA 421

Query: 439 IVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAM 498
             E  RR  A         E+   +SA W LP  I  G++E  + + Q EFFY ELP+ M
Sbjct: 422 ATEYARRKTA-------RDESGTKLSAMWLLPYMILGGIAEALNTIAQNEFFYSELPKTM 477

Query: 499 SSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVF 558
           SSVAT+L  L M+  +L +S+I+TIVD    T G  SW++ NI+ GH DYYYWLL  L  
Sbjct: 482 SSVATTLSSLNMAAASLISSWIITIVD--VTTYG--SWITENIDEGHLDYYYWLLVGLSL 477

Query: 559 ANFLYFLVCSKSYG 564
            N LYF+ C KSYG
Sbjct: 542 LNVLYFVWCKKSYG 477

BLAST of MC08g0314 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 2.6e-93
Identity = 199/544 (36.58%), Postives = 318/544 (58.46%), Query Frame = 0

Query: 31  KGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIFLWSAASNFTPII 90
           KGGL T+PFI AN   EK+A  G   +MI YLT    +    A+  +  ++  S+ TP++
Sbjct: 24  KGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLL 83

Query: 91  CAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCDELSGHCNAPSTPQLLLL 150
            AF+ADS+ GRF  I   S++  +GM +L ++A IP  RP   +    C    T QL +L
Sbjct: 84  GAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEVCVVADTAQLSIL 143

Query: 151 YSSYALMAIGSGCLQSSSIAFGADQLLKS--SGTLK--NYFSLVYMAQSAGVLVGMSCIV 210
           Y +  L A+GSG ++   +AFGADQ  +S  + T K  NYF+  Y    A VL+ ++ +V
Sbjct: 144 YVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLV 203

Query: 211 YIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGLVQVVVASYKKRRMK 270
           +IQD+VGW +G G+P   M  S IAF+    LY   +P+ S  T L+QV VA+++KR+++
Sbjct: 204 WIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLR 263

Query: 271 VPSTDTYEIYHHNNGSP-----HLMPSDKLRFLNKACIISNSEEELTSDGRASNPWSLCT 330
           + S  +   ++    +P      L  +  + FL+KA I+  +EE+    G+  N W L T
Sbjct: 264 MVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIV--TEEDNLKPGQIPNHWRLST 323

Query: 331 VDQVEGLKALIRIVPLWSTGILVAVGVNQ--SFYVLQLASMDRHLTPTFEVPAGSFGVVL 390
           V +VE LK++IR+ P+ ++GIL+     Q  +F + Q  +M+RHLT +F++PAGS  V  
Sbjct: 324 VHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFT 383

Query: 391 IVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVATAIVETNRRAMA 450
            V+++  II YDR+ + +  K  G +  ++   RMGIG +    + +    VE  R+++A
Sbjct: 384 TVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVA 443

Query: 451 IKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMSSVATSLFGL 510
           I+ G  D P  +V +S  W +P+Y   G++E F ++G +EFFY + P +M S AT+LF +
Sbjct: 444 IEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWM 503

Query: 511 GMSVGNLAASFIMTIVDNFSKTGGGESWV-STNINNGHCDYYYWLLFALVFANFLYFLVC 563
            +S+GN  ++ ++T+V  FS    G +W+   N+N G  +Y+YWL+  L   N +Y+L C
Sbjct: 504 AISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWC 563

BLAST of MC08g0314 vs. NCBI nr
Match: XP_022131878.1 (protein NRT1/ PTR FAMILY 1.2-like [Momordica charantia])

HSP 1 Score: 1128 bits (2917), Expect = 0.0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG
Sbjct: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ
Sbjct: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120

Query: 128 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF 187
           ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF
Sbjct: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF 180

Query: 188 SLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS 247
           SLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS
Sbjct: 181 SLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS 240

Query: 248 LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL 307
           LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL
Sbjct: 241 LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL 300

Query: 308 TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT 367
           TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT
Sbjct: 301 TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT 360

Query: 368 FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVA 427
           FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVA
Sbjct: 361 FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVA 420

Query: 428 TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR 487
           TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR
Sbjct: 421 TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR 480

Query: 488 AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL 547
           AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL
Sbjct: 481 AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL 540

Query: 548 VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 583
           VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH
Sbjct: 541 VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 576

BLAST of MC08g0314 vs. NCBI nr
Match: XP_038885652.1 (protein NRT1/ PTR FAMILY 1.2 isoform X4 [Benincasa hispida])

HSP 1 Score: 868 bits (2244), Expect = 5.01e-313
Identity = 440/579 (75.99%), Postives = 492/579 (84.97%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME PS AD+DRDMEEPLL ++D KGGLRTLPFIIAN ALEK+ASQGL+PSMILYLT VYG
Sbjct: 1   MEGPSVADDDRDMEEPLLSTADHKGGLRTLPFIIANGALEKLASQGLSPSMILYLTMVYG 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           M+SA AS +IFLWSAA+NFTPIICAFLADSY GRFP+IAAGS+ SFLGMFVLWLTA IPQ
Sbjct: 61  MKSAYASNIIFLWSAATNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQ 120

Query: 128 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSS----GTL 187
           ARPFCDE+SG C+APS  QL LLYSSYA+M+IGSGCLQSS +AFGADQL ++S    G L
Sbjct: 121 ARPFCDEISGRCDAPSRAQLSLLYSSYAIMSIGSGCLQSSYLAFGADQLYRTSKSNSGIL 180

Query: 188 KNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 247
            +YF+L Y++ + G LVGMS +VYIQD +GW MGFGVPVA ML S I F SASPLY+KSM
Sbjct: 181 DSYFNLCYISSAVGSLVGMSGMVYIQDRMGWGMGFGVPVALMLLSMITFFSASPLYLKSM 240

Query: 248 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 307
           PS S C G+VQVVVA+ KKR M+VPS    E YHH NGSP  +PSDKLRF NKACII NS
Sbjct: 241 PSRSWCAGIVQVVVAACKKRHMQVPSVGIPETYHHENGSPCALPSDKLRFFNKACIIRNS 300

Query: 308 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 367
           EEELTSD RASNPW+LCTV+QVE LKAL+RI+PLWSTGILV+  ++QSFY LQ+ASMDRH
Sbjct: 301 EEELTSDRRASNPWTLCTVEQVEDLKALVRIIPLWSTGILVSAALSQSFYTLQVASMDRH 360

Query: 368 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTF 427
           LTP+FEVPAGSFG +L+VSLIIWI LY+R+ILPL SK RGK TRLS KTRMGIGILF T 
Sbjct: 361 LTPSFEVPAGSFGAMLVVSLIIWITLYNRVILPLASKCRGKPTRLSGKTRMGIGILFSTL 420

Query: 428 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 487
           SL  +AIVE+NRRA+AIKEGFSDDP AVV+MSAFWTLPRYI  GM EGF+A+GQIEFFY 
Sbjct: 421 SLAVSAIVESNRRALAIKEGFSDDPNAVVNMSAFWTLPRYILFGMGEGFNAIGQIEFFYN 480

Query: 488 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 547
           ELP+AMSSVATSL GL MSVGNLAASFIMT VDNFSK    +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLLGLNMSVGNLAASFIMTTVDNFSKAAEVKSWVSSNINKGHSDYYYWL 540

Query: 548 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENNIL 582
           LF L+FANFLYFL CSKSYGPSKEEA   SNAED NN  
Sbjct: 541 LFGLLFANFLYFLACSKSYGPSKEEAGGGSNAEDHNNTF 579

BLAST of MC08g0314 vs. NCBI nr
Match: XP_038885651.1 (protein NRT1/ PTR FAMILY 1.2 isoform X3 [Benincasa hispida])

HSP 1 Score: 868 bits (2244), Expect = 1.36e-312
Identity = 440/579 (75.99%), Postives = 492/579 (84.97%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME PS AD+DRDMEEPLL ++D KGGLRTLPFIIAN ALEK+ASQGL+PSMILYLT VYG
Sbjct: 1   MEGPSVADDDRDMEEPLLSTADHKGGLRTLPFIIANGALEKLASQGLSPSMILYLTMVYG 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           M+SA AS +IFLWSAA+NFTPIICAFLADSY GRFP+IAAGS+ SFLGMFVLWLTA IPQ
Sbjct: 61  MKSAYASNIIFLWSAATNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQ 120

Query: 128 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSS----GTL 187
           ARPFCDE+SG C+APS  QL LLYSSYA+M+IGSGCLQSS +AFGADQL ++S    G L
Sbjct: 121 ARPFCDEISGRCDAPSRAQLSLLYSSYAIMSIGSGCLQSSYLAFGADQLYRTSKSNSGIL 180

Query: 188 KNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 247
            +YF+L Y++ + G LVGMS +VYIQD +GW MGFGVPVA ML S I F SASPLY+KSM
Sbjct: 181 DSYFNLCYISSAVGSLVGMSGMVYIQDRMGWGMGFGVPVALMLLSMITFFSASPLYLKSM 240

Query: 248 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 307
           PS S C G+VQVVVA+ KKR M+VPS    E YHH NGSP  +PSDKLRF NKACII NS
Sbjct: 241 PSRSWCAGIVQVVVAACKKRHMQVPSVGIPETYHHENGSPCALPSDKLRFFNKACIIRNS 300

Query: 308 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 367
           EEELTSD RASNPW+LCTV+QVE LKAL+RI+PLWSTGILV+  ++QSFY LQ+ASMDRH
Sbjct: 301 EEELTSDRRASNPWTLCTVEQVEDLKALVRIIPLWSTGILVSAALSQSFYTLQVASMDRH 360

Query: 368 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTF 427
           LTP+FEVPAGSFG +L+VSLIIWI LY+R+ILPL SK RGK TRLS KTRMGIGILF T 
Sbjct: 361 LTPSFEVPAGSFGAMLVVSLIIWITLYNRVILPLASKCRGKPTRLSGKTRMGIGILFSTL 420

Query: 428 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 487
           SL  +AIVE+NRRA+AIKEGFSDDP AVV+MSAFWTLPRYI  GM EGF+A+GQIEFFY 
Sbjct: 421 SLAVSAIVESNRRALAIKEGFSDDPNAVVNMSAFWTLPRYILFGMGEGFNAIGQIEFFYN 480

Query: 488 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 547
           ELP+AMSSVATSL GL MSVGNLAASFIMT VDNFSK    +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLLGLNMSVGNLAASFIMTTVDNFSKAAEVKSWVSSNINKGHSDYYYWL 540

Query: 548 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENNIL 582
           LF L+FANFLYFL CSKSYGPSKEEA   SNAED NN  
Sbjct: 541 LFGLLFANFLYFLACSKSYGPSKEEAGGGSNAEDHNNTF 579

BLAST of MC08g0314 vs. NCBI nr
Match: XP_008445024.1 (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucumis melo])

HSP 1 Score: 862 bits (2227), Expect = 1.55e-310
Identity = 435/581 (74.87%), Postives = 491/581 (84.51%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSD-GKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVY 67
           ME P   DE+R MEEPLL  +D GKGG+RTLPFIIAN ALEK+ASQGL+PSMILYLT+VY
Sbjct: 1   MEDPLVVDENRGMEEPLLSDTDDGKGGVRTLPFIIANGALEKLASQGLSPSMILYLTQVY 60

Query: 68  GMESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIP 127
           GM+SA AS VIFLWSAASNFTPIICAFLADSY GRFP++AAGS+ SFLGMFVLWLT  IP
Sbjct: 61  GMKSAQASNVIFLWSAASNFTPIICAFLADSYFGRFPMVAAGSIFSFLGMFVLWLTVMIP 120

Query: 128 QARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKS----SGT 187
           QARP CDE++GHCNAPS PQLLLLYSSYA+M+IGSGCLQ+S +AFGADQL +     SG 
Sbjct: 121 QARPLCDEINGHCNAPSIPQLLLLYSSYAIMSIGSGCLQASYLAFGADQLYRKNKSKSGI 180

Query: 188 LKNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKS 247
           L NYF++ Y++ + G LVGMSCIVYIQD +GW MGFGVPV  ML ++I FLSAS LY+KS
Sbjct: 181 LDNYFNICYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVVLMLLATITFLSASSLYLKS 240

Query: 248 MPSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISN 307
           MPS S C GLVQVV A+YKKR M++    T  +YHH NGS   +PSDKLRFLNKACII N
Sbjct: 241 MPSKSWCAGLVQVVFAAYKKRHMQIAFVGTSGMYHHENGSLCALPSDKLRFLNKACIIRN 300

Query: 308 SEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDR 367
           SEEELTSDG+ASNPWSLCTV+QVE LKALI+I+PLWSTGILV+  +NQSFYVLQLASMDR
Sbjct: 301 SEEELTSDGKASNPWSLCTVEQVENLKALIKIIPLWSTGILVSASLNQSFYVLQLASMDR 360

Query: 368 HLTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCT 427
           HLT +FEVPAGSFG +L+VSLIIWI LYDRLILPL SK RGK TRLS KTRMG+GIL CT
Sbjct: 361 HLTSSFEVPAGSFGAMLVVSLIIWITLYDRLILPLASKCRGKPTRLSAKTRMGLGILCCT 420

Query: 428 FSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFY 487
            SL  +A+VE  RRA+AI+EGFSDDP AVVSMSAFWTLPRYI  GM+E F+A+GQIEFFY
Sbjct: 421 LSLAVSAVVEGYRRALAIREGFSDDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFY 480

Query: 488 FELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYW 547
            ELP+AMSSVATSL GL  SVGNLAASFIMT VDNFSK  GG+SWVS+NIN+GH DYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYW 540

Query: 548 LLFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 583
           LLF L+FAN LYFL CSKSYGPSKEE+   S AED NN ++
Sbjct: 541 LLFGLLFANLLYFLACSKSYGPSKEESGGRSTAEDYNNAVN 581

BLAST of MC08g0314 vs. NCBI nr
Match: XP_023546647.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 860 bits (2222), Expect = 7.98e-310
Identity = 438/579 (75.65%), Postives = 491/579 (84.80%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME P  ADEDRDMEEPLL +SD KGGLR LPFIIAN ALE++ASQGL+PSMILYLTRVYG
Sbjct: 1   MEGPLAADEDRDMEEPLLSNSDRKGGLRALPFIIANGALERLASQGLSPSMILYLTRVYG 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           M SA AS VIFLWSAASNFTPIICAFLADSY GRF  IAAG ++S LGMFVLWLTA IPQ
Sbjct: 61  MNSANASNVIFLWSAASNFTPIICAFLADSYFGRFRTIAAGCIISCLGMFVLWLTAMIPQ 120

Query: 128 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGTL 187
           ARPFCD     C+ PS  QLL LYSSYALM++GSGCLQSS++AFGADQL +    +SG L
Sbjct: 121 ARPFCD--GAICDTPSIAQLLFLYSSYALMSMGSGCLQSSNLAFGADQLHRRNKSNSGIL 180

Query: 188 KNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 247
             YF L Y++ ++G L+G+SCIVYIQD +GW +GFGVPVA ML S++ FL ASPLY+K M
Sbjct: 181 DRYFDLYYVSSASGSLIGVSCIVYIQDRMGWRVGFGVPVALMLLSTVIFLLASPLYLKPM 240

Query: 248 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 307
           PSSS C GLVQV VA+YKKR M+V ST T E+YHH NGSP  MPSDKLRFLNKACII NS
Sbjct: 241 PSSSWCAGLVQVFVAAYKKRHMQVSSTGTSEMYHHKNGSPLAMPSDKLRFLNKACIIRNS 300

Query: 308 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 367
           E+EL SDGRASNPW LCTV+QVE LK LIRI+ LWSTGILV   ++Q FYVLQ+ASMDRH
Sbjct: 301 EDELASDGRASNPWILCTVEQVEDLKTLIRIMALWSTGILVCAALSQPFYVLQVASMDRH 360

Query: 368 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTF 427
           LTPTFEVPAGSFG V +VSLI+WI+LYDRLILPLVS  RGK TRLS KTRMGIGIL CTF
Sbjct: 361 LTPTFEVPAGSFGAVFVVSLILWIMLYDRLILPLVSSCRGKPTRLSGKTRMGIGILLCTF 420

Query: 428 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 487
           SL  TA+VE+NRRA+AIKEGFSDDP+AVV+MSAFWTLPRYI LGM+E F+ +GQIEFFY+
Sbjct: 421 SLAVTAVVESNRRALAIKEGFSDDPDAVVNMSAFWTLPRYILLGMAEAFNVIGQIEFFYY 480

Query: 488 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 547
           ELP+AMSSVATSLFGL MSVGNLAASFI+TIVDNF+K  G +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLFGLSMSVGNLAASFIVTIVDNFTKAAGVKSWVSSNINQGHSDYYYWL 540

Query: 548 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDE-NNI 581
           LF L+FANFLYFL CSKSYGPSKEEA   SNAED+ NN+
Sbjct: 541 LFGLLFANFLYFLACSKSYGPSKEEARGGSNAEDDLNNV 577

BLAST of MC08g0314 vs. ExPASy TrEMBL
Match: A0A6J1BUQ4 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004912 PE=3 SV=1)

HSP 1 Score: 1128 bits (2917), Expect = 0.0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG
Sbjct: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ
Sbjct: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120

Query: 128 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF 187
           ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF
Sbjct: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF 180

Query: 188 SLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS 247
           SLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS
Sbjct: 181 SLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS 240

Query: 248 LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL 307
           LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL
Sbjct: 241 LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL 300

Query: 308 TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT 367
           TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT
Sbjct: 301 TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT 360

Query: 368 FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVA 427
           FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVA
Sbjct: 361 FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVA 420

Query: 428 TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR 487
           TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR
Sbjct: 421 TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR 480

Query: 488 AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL 547
           AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL
Sbjct: 481 AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL 540

Query: 548 VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 583
           VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH
Sbjct: 541 VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 576

BLAST of MC08g0314 vs. ExPASy TrEMBL
Match: A0A1S3BBQ7 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488189 PE=3 SV=1)

HSP 1 Score: 862 bits (2227), Expect = 7.50e-311
Identity = 435/581 (74.87%), Postives = 491/581 (84.51%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSD-GKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVY 67
           ME P   DE+R MEEPLL  +D GKGG+RTLPFIIAN ALEK+ASQGL+PSMILYLT+VY
Sbjct: 1   MEDPLVVDENRGMEEPLLSDTDDGKGGVRTLPFIIANGALEKLASQGLSPSMILYLTQVY 60

Query: 68  GMESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIP 127
           GM+SA AS VIFLWSAASNFTPIICAFLADSY GRFP++AAGS+ SFLGMFVLWLT  IP
Sbjct: 61  GMKSAQASNVIFLWSAASNFTPIICAFLADSYFGRFPMVAAGSIFSFLGMFVLWLTVMIP 120

Query: 128 QARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKS----SGT 187
           QARP CDE++GHCNAPS PQLLLLYSSYA+M+IGSGCLQ+S +AFGADQL +     SG 
Sbjct: 121 QARPLCDEINGHCNAPSIPQLLLLYSSYAIMSIGSGCLQASYLAFGADQLYRKNKSKSGI 180

Query: 188 LKNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKS 247
           L NYF++ Y++ + G LVGMSCIVYIQD +GW MGFGVPV  ML ++I FLSAS LY+KS
Sbjct: 181 LDNYFNICYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVVLMLLATITFLSASSLYLKS 240

Query: 248 MPSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISN 307
           MPS S C GLVQVV A+YKKR M++    T  +YHH NGS   +PSDKLRFLNKACII N
Sbjct: 241 MPSKSWCAGLVQVVFAAYKKRHMQIAFVGTSGMYHHENGSLCALPSDKLRFLNKACIIRN 300

Query: 308 SEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDR 367
           SEEELTSDG+ASNPWSLCTV+QVE LKALI+I+PLWSTGILV+  +NQSFYVLQLASMDR
Sbjct: 301 SEEELTSDGKASNPWSLCTVEQVENLKALIKIIPLWSTGILVSASLNQSFYVLQLASMDR 360

Query: 368 HLTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCT 427
           HLT +FEVPAGSFG +L+VSLIIWI LYDRLILPL SK RGK TRLS KTRMG+GIL CT
Sbjct: 361 HLTSSFEVPAGSFGAMLVVSLIIWITLYDRLILPLASKCRGKPTRLSAKTRMGLGILCCT 420

Query: 428 FSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFY 487
            SL  +A+VE  RRA+AI+EGFSDDP AVVSMSAFWTLPRYI  GM+E F+A+GQIEFFY
Sbjct: 421 LSLAVSAVVEGYRRALAIREGFSDDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFY 480

Query: 488 FELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYW 547
            ELP+AMSSVATSL GL  SVGNLAASFIMT VDNFSK  GG+SWVS+NIN+GH DYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYW 540

Query: 548 LLFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 583
           LLF L+FAN LYFL CSKSYGPSKEE+   S AED NN ++
Sbjct: 541 LLFGLLFANLLYFLACSKSYGPSKEESGGRSTAEDYNNAVN 581

BLAST of MC08g0314 vs. ExPASy TrEMBL
Match: A0A6J1HCS0 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita moschata OX=3662 GN=LOC111462799 PE=3 SV=1)

HSP 1 Score: 858 bits (2218), Expect = 1.57e-309
Identity = 437/579 (75.47%), Postives = 493/579 (85.15%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME P  ADEDRDMEEPLL +SD KGGLR LPFIIAN ALE++ASQGL+PSMILYLTRVYG
Sbjct: 1   MEGPLAADEDRDMEEPLLSNSDRKGGLRALPFIIANGALERLASQGLSPSMILYLTRVYG 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           M SA AS VIFLWSAASNFTPIICAFLADSY GRF +IAAG ++S LGMFVLWLTA IPQ
Sbjct: 61  MNSANASNVIFLWSAASNFTPIICAFLADSYFGRFRMIAAGCIISCLGMFVLWLTAMIPQ 120

Query: 128 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGTL 187
           ARPFCD     C+ PS  QLLLLYSSYALM++GSGCLQSS++AFGA+QL +    +SG L
Sbjct: 121 ARPFCD--GAICDTPSIAQLLLLYSSYALMSMGSGCLQSSNLAFGANQLHRKNKSNSGIL 180

Query: 188 KNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 247
             YF L Y++ ++G L+G+SCIVYIQD +GW +GFGVPVA ML S++ FL ASPLY+K M
Sbjct: 181 DRYFDLYYVSSASGSLIGVSCIVYIQDRMGWRVGFGVPVALMLLSTVIFLLASPLYLKPM 240

Query: 248 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 307
           PSSS C GLVQV VA+YKKR M+V ST T E+YHH +GSP  MPSDKLRFLNKACII NS
Sbjct: 241 PSSSWCAGLVQVFVAAYKKRHMQVSSTGTSEMYHHKSGSPLAMPSDKLRFLNKACIIRNS 300

Query: 308 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 367
           E+EL SDGRASNPW LCTV+QVE LK LIRI+ LWSTGILV   ++Q FYVLQ+ASMDRH
Sbjct: 301 EDELASDGRASNPWILCTVEQVEDLKTLIRIMALWSTGILVCAALSQPFYVLQVASMDRH 360

Query: 368 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTF 427
           LTPTFEVPAGSFG V +VSLI+WI+LYDRLILPLVS  RGK TRLS KTRMGIGIL CTF
Sbjct: 361 LTPTFEVPAGSFGAVFVVSLILWIMLYDRLILPLVSSCRGKPTRLSGKTRMGIGILLCTF 420

Query: 428 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 487
           SL  TA+VE+NRRA+AIKEGFSDDP+AVV+MSAFWTLPRYI LGM+E F+ +GQIEFFY+
Sbjct: 421 SLAVTAVVESNRRALAIKEGFSDDPDAVVNMSAFWTLPRYILLGMAEAFNVIGQIEFFYY 480

Query: 488 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 547
           ELP+AMSSVATSLFGL MSVGNLAASFI+TIVDNF+K  G +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLFGLSMSVGNLAASFIVTIVDNFTKAAGVKSWVSSNINQGHSDYYYWL 540

Query: 548 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDE-NNI 581
           LF L+FANFLYFL CSKSYGPSKEEA   SNAED+ NN+
Sbjct: 541 LFGLLFANFLYFLACSKSYGPSKEEARGGSNAEDDLNNV 577

BLAST of MC08g0314 vs. ExPASy TrEMBL
Match: A0A6J1KBF9 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita maxima OX=3661 GN=LOC111491785 PE=3 SV=1)

HSP 1 Score: 858 bits (2217), Expect = 2.23e-309
Identity = 438/579 (75.65%), Postives = 489/579 (84.46%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME P  ADEDRDMEEPLL +SD K GLR LPFIIAN ALEK+ASQGL+PSMILYLTRVYG
Sbjct: 1   MEGPLAADEDRDMEEPLLSNSDRKEGLRALPFIIANGALEKLASQGLSPSMILYLTRVYG 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           M SA AS VIFLWSAASNFTPIICAFLADSY GRF +IAAG ++S LGMFVLWLTA IPQ
Sbjct: 61  MNSANASNVIFLWSAASNFTPIICAFLADSYFGRFRMIAAGCIISCLGMFVLWLTAMIPQ 120

Query: 128 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGTL 187
           ARPFCD     C+ PS  QLLLLYSSYALM+IGSGCLQSS++AFGADQL +    +SG L
Sbjct: 121 ARPFCD--GAICDTPSIAQLLLLYSSYALMSIGSGCLQSSNLAFGADQLHRKNKSNSGIL 180

Query: 188 KNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 247
             YF L Y++ ++G L+G+SCIVYIQD +GW +GFGVPVA ML S++ FL ASPLY+K M
Sbjct: 181 DRYFDLYYVSSASGSLIGVSCIVYIQDRMGWRVGFGVPVALMLLSTVIFLLASPLYLKPM 240

Query: 248 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 307
           PSSS C GLVQV VA+YKKR M+V ST T E+YHH NGSP  MPSDKLRFLNKACII NS
Sbjct: 241 PSSSWCAGLVQVFVAAYKKRHMQVSSTGTSEMYHHKNGSPLAMPSDKLRFLNKACIIRNS 300

Query: 308 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 367
           E+EL SDGRASNPW LCTV+QVE LK LIRI+ LWSTGILV   ++Q FYVLQ+ASMDRH
Sbjct: 301 EDELASDGRASNPWILCTVEQVEDLKTLIRIMALWSTGILVCAALSQPFYVLQVASMDRH 360

Query: 368 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTF 427
           LTPTFE+PAGSFG V +VSLI+WI+LYDRLILPLVS  RGK TRLS KTRMGIGIL CTF
Sbjct: 361 LTPTFEIPAGSFGAVFVVSLILWIMLYDRLILPLVSSCRGKPTRLSGKTRMGIGILLCTF 420

Query: 428 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 487
           SL  TA+VE+NRRA+AIKEGFSDDP AVV+MSAFWTLPRYI LGM+E F+ +GQIEFFY+
Sbjct: 421 SLAVTAVVESNRRALAIKEGFSDDPAAVVNMSAFWTLPRYILLGMAEAFNVIGQIEFFYY 480

Query: 488 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 547
           ELP+AMSSVATSLFGL MSVGNL ASFIMT VDNF+K  G +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLFGLSMSVGNLTASFIMTTVDNFTKAAGVKSWVSSNINQGHSDYYYWL 540

Query: 548 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDE-NNI 581
           LF LVFANFLYFL CS+SYGPSKEEA   SNAED+ NN+
Sbjct: 541 LFGLVFANFLYFLACSRSYGPSKEEARGGSNAEDDLNNV 577

BLAST of MC08g0314 vs. ExPASy TrEMBL
Match: A0A1S4DVD3 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo OX=3656 GN=LOC103488191 PE=3 SV=1)

HSP 1 Score: 838 bits (2166), Expect = 1.43e-301
Identity = 424/581 (72.98%), Postives = 490/581 (84.34%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSD-GKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVY 67
           ME     DE+R MEEPLL  +D GKGGLRTLPFIIAN   EKVAS GL+PSMILYLT VY
Sbjct: 1   MEDQLVVDENRGMEEPLLSDTDDGKGGLRTLPFIIANGTFEKVASHGLSPSMILYLTEVY 60

Query: 68  GMESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIP 127
           GM+SA AS VIFLWSAA+NFTPIICAFLADSY GRF +IAAGS+ SFLGMFVLWLTA IP
Sbjct: 61  GMKSAHASNVIFLWSAATNFTPIICAFLADSYFGRFSMIAAGSIFSFLGMFVLWLTAIIP 120

Query: 128 QARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGT 187
           Q RPFCD+++G+C+APSTPQLLLLYSSYA+M+IGSGCLQ+S +AFGADQL K    +SG 
Sbjct: 121 QVRPFCDKINGYCDAPSTPQLLLLYSSYAIMSIGSGCLQASHLAFGADQLYKESKSNSGI 180

Query: 188 LKNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKS 247
           L +YF++ Y++ + G L+GMSCIVYIQD +GW +GFGV VA ML ++I FLSAS LY+KS
Sbjct: 181 LNSYFNICYISIAVGTLIGMSCIVYIQDRMGWGIGFGVSVALMLLATITFLSASSLYLKS 240

Query: 248 MPSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISN 307
           MPS S C GLVQVV A+YKKR M++P   T+E+YHH  GS   +PS+KLRFLNKACII N
Sbjct: 241 MPSKSWCDGLVQVVFATYKKRHMQIPFVGTFEMYHHETGSLCALPSNKLRFLNKACIIRN 300

Query: 308 SEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDR 367
            EEELTSDG+ASNPWSLCTV+QVE LKALIRI+PLWSTGIL +V ++QSFYVLQ+ASMDR
Sbjct: 301 FEEELTSDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILFSVSLSQSFYVLQVASMDR 360

Query: 368 HLTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCT 427
           HLT +FEVPAGSF V+++V +IIWIILY+ LILPL SK RGK T LS KTRMG+GIL CT
Sbjct: 361 HLTSSFEVPAGSFSVLIVVFVIIWIILYNCLILPLASKCRGKPTHLSGKTRMGLGILCCT 420

Query: 428 FSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFY 487
            SL  +AIVE +RRA+AIKEGFS+DP AVVSMSAFWTLPRYI  GM+E F+A+GQIEFFY
Sbjct: 421 LSLAVSAIVEGDRRALAIKEGFSNDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFY 480

Query: 488 FELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYW 547
            ELP+AMSSVATSL GL  SVGNLAASFIMT VDNFSK  GG+SWVS+NIN+GH DYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYW 540

Query: 548 LLFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 583
           LLF L+FANFLYFL CSKSYGPSKEE++  S AED NN ++
Sbjct: 541 LLFGLLFANFLYFLACSKSYGPSKEESAGRSTAEDYNNTVN 581

BLAST of MC08g0314 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 552.7 bits (1423), Expect = 3.4e-157
Identity = 286/570 (50.18%), Postives = 400/570 (70.18%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME P    E + ++    K +  KGG+ T+PFIIAN A EKVAS GL P+MI+YL R Y 
Sbjct: 1   MENPPNETEAKQIQTNEGKKT--KGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYR 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
              A  + V+F+WSAASNFTP++ AFL+DSYLGRF  I+  S+ SFLGM +LWLTA +PQ
Sbjct: 61  FGVAKGTNVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQ 120

Query: 128 ARPF-CDELS--GHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQL-----LKS 187
            +P  CD  +   HC + +  QL LLYS++AL++IGSG ++  S+AFGADQL      K+
Sbjct: 121 VKPSPCDPTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKN 180

Query: 188 SGTLKNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLY 247
              L+++F   Y + +  VL+  + IVYIQ+H+GW +GFGVP   ML +++ F+ ASPLY
Sbjct: 181 ERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLY 240

Query: 248 VKSMPSSSLCTGLVQVVVASYKKRRMKVPS-TDTYEIYHHNNGSPHLMPSDKLRFLNKAC 307
           V    + SL TGL Q +VA+YKKR++ +P   D+++ Y+H   S    PS KLRFLNKAC
Sbjct: 241 VTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKAC 300

Query: 308 IISNSEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQL 367
           +ISN EEE+ SDG A NPW LCT D+VE LKALI+++P+WSTGI++++  +Q SF +LQ 
Sbjct: 301 LISNREEEIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQA 360

Query: 368 ASMDRHLT---PTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRM 427
            SMDR L+    +F+VPAGSFG+  I++L +W+ILYDR ++PL SKIRG+  RLS K RM
Sbjct: 361 TSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRM 420

Query: 428 GIGILFCTFSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSA 487
           G+G+     ++  +A+VE+ RR  AI +G++++  AVV +SA W +P+Y+  G++E  +A
Sbjct: 421 GLGLFMSFLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTA 480

Query: 488 VGQIEFFYFELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINN 547
           +GQ EFFY E P++MSS+A SLFGLGM+V +L AS ++  V+  +   G ESWVS NIN 
Sbjct: 481 IGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINK 540

Query: 548 GHCDYYYWLLFALVFANFLYFLVCSKSYGP 565
           GH +YYYW+L  + F N +Y+++CS SYGP
Sbjct: 541 GHYNYYYWVLAIMSFINVIYYVICSWSYGP 568

BLAST of MC08g0314 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 534.3 bits (1375), Expect = 1.3e-151
Identity = 284/576 (49.31%), Postives = 389/576 (67.53%), Query Frame = 0

Query: 8   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 67
           ME P    E ++  +  +     KGGL T+PFIIAN   EKVAS GL  +MILYL   Y 
Sbjct: 1   MENPPDQTESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYR 60

Query: 68  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 127
           +      TV+F+W AA+NF P++ AFL+DSYLGRF  I   S+ S LGM VLWLTA +PQ
Sbjct: 61  LGLVKGQTVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQ 120

Query: 128 ARPF-CDELSG-HCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQL-----LKSS 187
            +P  C   +G +C++ ++ QL LLY+++AL++IGSG ++  S+AFGADQL      K+ 
Sbjct: 121 VKPSPCVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNE 180

Query: 188 GTLKNYFSLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYV 247
             L+++F   Y + S  VL+  + IVYIQDH+GW +GFG+P   ML +   F+ ASPLYV
Sbjct: 181 RVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYV 240

Query: 248 KSMPSSSLCTGLVQVVVASYKKRRMKVPS-TDTYEIYHHNNGSPHLMPSDKLRFLNKACI 307
           K   S SL TGL QVV A+Y KR + +P   D+ + Y+    S    PSDKLRFLNKAC 
Sbjct: 241 KRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACA 300

Query: 308 ISNSEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQLA 367
           ISN +E+L SDG A N W LCT DQVE LKAL++++P+WSTGI++++ V+Q SF +LQ  
Sbjct: 301 ISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAK 360

Query: 368 SMDRHLT--PTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGI 427
           SMDR L+   TF++PAGSFG+  I++LI W++LYDR ILPL SKIRG+  R++ K RMG+
Sbjct: 361 SMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGL 420

Query: 428 GILFCTFSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVG 487
           G+     ++  +A VE  RR  AI +G ++D  + VS+SA W +P+Y+  G++E  + +G
Sbjct: 421 GLFISFLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIG 480

Query: 488 QIEFFYFELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGH 547
           Q EFFY E P++MSS+A SLFGLGM+V N+ AS I+  V N SK  G  SW+  NIN GH
Sbjct: 481 QTEFFYTEFPKSMSSIAASLFGLGMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGH 540

Query: 548 CDYYYWLLFALVFANFLYFLVCSKSYGPSKEEASDE 573
            DYYYW+L  L F N +Y++VCS SYGP+ ++  ++
Sbjct: 541 YDYYYWVLAILSFVNVIYYVVCSWSYGPTVDQVRND 575

BLAST of MC08g0314 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 355.1 bits (910), Expect = 1.0e-97
Identity = 200/565 (35.40%), Postives = 329/565 (58.23%), Query Frame = 0

Query: 32  GGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIFLWSAASNFTPIIC 91
           GG R + FI+ N  LE++ S GL  + ++YLT+V+ +E   A+ VI +WS  +N TP++ 
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 92  AFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFC----DELSGHCNAPSTPQL 151
           A+++D+Y+GRF  IA  S  + LG+  + LTA+ PQ  P      D LS  C  P+  Q+
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLS--CGGPNKLQI 172

Query: 152 LLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGT----LKNYFSLVYMAQSAGVLVGMS 211
            +L      +++GSG ++  SI FG DQ  + +      + ++F+  YM  +  +++  +
Sbjct: 173 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQT 232

Query: 212 CIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGLVQVVVASYKKR 271
            +VYIQD V W +GF +P   M  + + F +    YV   P  S+ +G+ QV+VA+ KKR
Sbjct: 233 VVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKR 292

Query: 272 RMKVPSTDTYEIYHH-----NNGSPHLMPSDKLRFLNKACIISNSEEELTSDGRASNPWS 331
           ++K+P+ D   + ++     ++    L  S++ R L+KA ++   E +LT +G  ++ W 
Sbjct: 293 KLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWR 352

Query: 332 LCTVDQVEGLKALIRIVPLWSTGI--LVAVGVNQSFYVLQLASMDRHLTPTFEVPAGSFG 391
           LC+V +VE +K LIRIVP+WS GI  L A+    +F V Q   MDR+L P FE+PAGS  
Sbjct: 353 LCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLS 412

Query: 392 VVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVATAIVETNRR 451
           V+ ++++ I++  YDR+ +P + +I G ++ ++   R+G GI+F  FS++   IVE  RR
Sbjct: 413 VISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRR 472

Query: 452 AMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMSSVATSL 511
             +I  G   DP  +  MS FW  P+ I +G+ E F+ +GQIEFF  + P  M S+A SL
Sbjct: 473 IRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSL 532

Query: 512 FGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVFANFLYFL 571
           F L  +  +  +SF++T+V  FS       W++ N+N G  DY+Y+L+  L   N +YF 
Sbjct: 533 FSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFW 592

Query: 572 VCSKSY----GPSKEEASDESNAED 578
            C++ Y    G   E+  ++ +++D
Sbjct: 593 YCARGYRYKVGLPIEDFEEDKSSDD 610

BLAST of MC08g0314 vs. TAIR 10
Match: AT5G11570.1 (Major facilitator superfamily protein )

HSP 1 Score: 346.3 bits (887), Expect = 4.9e-95
Identity = 218/554 (39.35%), Postives = 305/554 (55.05%), Query Frame = 0

Query: 19  DMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIF 78
           D E  L+  +  K G+ T+PFI+A+ ALEK+A  GL P+MIL+LT  YGM +A A+ ++F
Sbjct: 2   DQEALLVGRTLLKRGIPTIPFILASQALEKLAYFGLVPNMILFLTVEYGMGTAEAANILF 61

Query: 79  LWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCDELSGH 138
           LWSAA+NF P++ AF+ADSY GRFP+I  GS +S  GM +LWLT  I   RP CD+L+  
Sbjct: 62  LWSAATNFFPLVGAFIADSYTGRFPLIGFGSSISLTGMVLLWLTTII---RPECDKLTNV 121

Query: 139 CNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK------SSGTLKNYFSLVYM 198
           C   +  + +LLYS +AL AIG+G ++SS +AF ADQL        ++ +L+  F+  Y 
Sbjct: 122 CQPTTLLKSVLLYSFFALTAIGAGGVRSSCLAFAADQLQPNQTSRVTTSSLETLFNWYYF 181

Query: 199 AQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGL 258
           +      +  S +V++Q   GW +GFGV VA M  S   F +ASP YV            
Sbjct: 182 SVMVACFLSQSLLVFVQTTYGWQIGFGVSVAAMALSVALFFAASPYYV------------ 241

Query: 259 VQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEELTSDGR 318
                      R + P+ +                                         
Sbjct: 242 -----------RFQKPTRN----------------------------------------- 301

Query: 319 ASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAV--GVNQSFYVLQLASMDRH-LTPTFE 378
           + NPW LC V QVE LK+LI ++P+WSTGI++++      SF VLQ  +MDRH     FE
Sbjct: 302 SRNPWKLCRVQQVEDLKSLINVIPIWSTGIILSLVTACQVSFIVLQAKTMDRHTFIQGFE 361

Query: 379 VPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVATA 438
           +P GS+G+ L++S ++++ LYD +I+PL+S    +  RL    RM  G +     + A A
Sbjct: 362 IPPGSYGIFLVISFLLFLGLYDLVIVPLLSWALREPFRLGVMVRMWAGYVISVLCISALA 421

Query: 439 IVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAM 498
             E  RR  A         E+   +SA W LP  I  G++E  + + Q EFFY ELP+ M
Sbjct: 422 ATEYARRKTA-------RDESGTKLSAMWLLPYMILGGIAEALNTIAQNEFFYSELPKTM 477

Query: 499 SSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVF 558
           SSVAT+L  L M+  +L +S+I+TIVD    T G  SW++ NI+ GH DYYYWLL  L  
Sbjct: 482 SSVATTLSSLNMAAASLISSWIITIVD--VTTYG--SWITENIDEGHLDYYYWLLVGLSL 477

Query: 559 ANFLYFLVCSKSYG 564
            N LYF+ C KSYG
Sbjct: 542 LNVLYFVWCKKSYG 477

BLAST of MC08g0314 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 1.8e-94
Identity = 199/544 (36.58%), Postives = 318/544 (58.46%), Query Frame = 0

Query: 31  KGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIFLWSAASNFTPII 90
           KGGL T+PFI AN   EK+A  G   +MI YLT    +    A+  +  ++  S+ TP++
Sbjct: 24  KGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLL 83

Query: 91  CAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCDELSGHCNAPSTPQLLLL 150
            AF+ADS+ GRF  I   S++  +GM +L ++A IP  RP   +    C    T QL +L
Sbjct: 84  GAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEVCVVADTAQLSIL 143

Query: 151 YSSYALMAIGSGCLQSSSIAFGADQLLKS--SGTLK--NYFSLVYMAQSAGVLVGMSCIV 210
           Y +  L A+GSG ++   +AFGADQ  +S  + T K  NYF+  Y    A VL+ ++ +V
Sbjct: 144 YVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLV 203

Query: 211 YIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGLVQVVVASYKKRRMK 270
           +IQD+VGW +G G+P   M  S IAF+    LY   +P+ S  T L+QV VA+++KR+++
Sbjct: 204 WIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLR 263

Query: 271 VPSTDTYEIYHHNNGSP-----HLMPSDKLRFLNKACIISNSEEELTSDGRASNPWSLCT 330
           + S  +   ++    +P      L  +  + FL+KA I+  +EE+    G+  N W L T
Sbjct: 264 MVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIV--TEEDNLKPGQIPNHWRLST 323

Query: 331 VDQVEGLKALIRIVPLWSTGILVAVGVNQ--SFYVLQLASMDRHLTPTFEVPAGSFGVVL 390
           V +VE LK++IR+ P+ ++GIL+     Q  +F + Q  +M+RHLT +F++PAGS  V  
Sbjct: 324 VHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFT 383

Query: 391 IVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVATAIVETNRRAMA 450
            V+++  II YDR+ + +  K  G +  ++   RMGIG +    + +    VE  R+++A
Sbjct: 384 TVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVA 443

Query: 451 IKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMSSVATSLFGL 510
           I+ G  D P  +V +S  W +P+Y   G++E F ++G +EFFY + P +M S AT+LF +
Sbjct: 444 IEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWM 503

Query: 511 GMSVGNLAASFIMTIVDNFSKTGGGESWV-STNINNGHCDYYYWLLFALVFANFLYFLVC 563
            +S+GN  ++ ++T+V  FS    G +W+   N+N G  +Y+YWL+  L   N +Y+L C
Sbjct: 504 AISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWC 563