MC08g0235 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0235
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Protein CutA
Location	MC08: 1720345 .. 1723385 (+)
RNA-Seq Expression	MC08g0235
Synteny	MC08g0235

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTCACTCTCTGCAGCAGAGTCTCGGCTCCACTGCTATCCTCCTCTGCCCTACGACGTCGTTTGCCCCTTGTTGGCGCGTTCTGCGTACTGAGCTTCGGAATTTCCCATATCTTCATCTCCAGAACGGGTTCCGCTCTGTCTCTGCCTTTTGCCCCTCTCTTGAGGTACTTCTTTTCGCTTCCTTGCTTTCTTTTTCTGATTCATTTCTGCTGGGATTGATTCTTGCTGAGATTCTGCCTTAGTTACTAGTTAATTTGTTTAGGTTCTTTATTTGATCTGGAAAAATATGTCAGATCGAAGTTTGGTGGTCAAGGACCAGCTAGGAACGTACATTTTGTAAGAATGGAAGCGACTAGCGCCGCGGTGCCTAGCATTGTTGTTTATGTCACCGTCCCGAATAGGGAAGCAGGTTCTCTACTATCTCTCTGCTCTAAAAGTCCCGCGTTCTTGGTGGTTTTGAATTCATTGATTGGTATTTTGTACATTTGACCAACTACGATCTGATTACAATATATATTTTAGTTGCTATAGTTTTTTTTTTTTCTTTCCGGTTTCCAGTATCTTGTGTACGGCTTGGACTAGATTGTTTGTTCGTCATCCTAGTGATAAGCCTATCAGGATCTGGAAAACATATCTTGTGTGTGGTTTGAAATCGTTATGAGTGGTTATGCAAGTAGCCTCTTCACTGTTAGGCGTTTTTGGAGGAAACTTATATTGGCTACTTATGGAGTTTTGTGTTGATTCGGAGTTATAAATATATCTAGCGCATTGGTGTGCGATGAAAATATGCACATAATGACCGGACTCGATAAGCAAATGATGAATTATGATTCAACAAGTGCTGTAATGTTATTATATTGCATATATTGTTTTTGAACATGTATCATGTATTGGCTTCTTCTGGTGACGATGAATGTCTTGCAATAATTGTGGTGGTTGTTTTTATTTTCCACTCACTTGGATGATTAACGTTCTGAAATAGTAGGCCTTTGAGAAAGTTCAGAGTTATTTTTAAATGTGTATTGCTCCCACTTCAGAAAATATTACAAATTTGATGATTTCTTCAGAAACTTTTTTGGACTGTGAGATGCTCCATCTAACTCTAGTTGTGATGCTTAAAACATATTACTTATTGCAGTTCCATTTTTCCTTTTCTTTTTCTACTTTTATTTTCTTAACTCTTAAGTATGGTGGATTCTTGGAGCTTAAGGCCTTTTGTTTTCTTTTTTTTTTTGTTTAGTTATTTCCGTTCTCAACTTCTTTCTTTCCCTTTTAATTTTGTTGGGACAGAACTAGTCCAATGTTTCAAATTCCGATTTCATTGCATTTCGTTGTGTTTCCTGCTATATCCACATTTCTAAAAAAAAAATTATAACGAAATGAAATATGTAACCCTGTAATGTAAGTGTCTAGCAAGTGTTGGATCAAATGTCTAATGCCCAAACTAAACTGTAATGTGTTTCATAAGATTGAGAGTGGTTAGGCTATGGGTGGAGAGAGGGGGAGAAAAGAAAAGTAACTAATTTTTCAATACCATGGAGTTTACTTCTTTTTAATAGATTTTATATTTCTTATCTTATTCACCCATTATTTCTTCAGGTAAGAAATTGGCTGAAAGCATTGTCAAAGAAAAGCTTGCTGCTTGTGTTAACATAGTACCTGGTATGTACATTTTCTCCACCTCATACTGATTTTCTCGTAAGCCTACAATTTTAAATTTCCATTTAAGTAATATATCAATGTCTCTGGAACATGTGGCAGAATGGATTGCTCCTAGTTTTTGAGAAGATATTATATATACAATATTTATAAGCTATATTTGCAGAACATTTTGATGTTCTCCTTTAAGTTCTCTATCCATATCGATGAAATTGCTTCTGACCATTTAAAATTGAAGCTTCCTGGATCTTCCCTTCCCTGTTATCTAAGTGTGCTGTTTTTCAAGGAAGGAAACTTGTTAGAAATAGTTTATTTGGTCATGTATGAAAGAACAATTACTTGTTTCTTTTGATCAATTAACTCATGAACTGGCTTCTATAAATTTCCAAAACTCTGTTTTAATTTTTAATCGTTTCAATTTCTTACCATTGTATTCCTTTTCTTTTCACCTTCTTTTCGTTCCATTGTAACTGTTAATCTGAATATGCATTCTCAGACTTCTGATATATGAATACTTTATAAATGCAGGTATAGAATCTGTCTACCAGTGGAAGGGAGAGGTGTGGTTCTCATTTTCGCGAATCAATATTTAGTCATAAATATATGATTGCTTGTTTGTCTTTATCAAAAAATAACTTCAATTGTAGGCTTTTCTATTTCTGGTTGACAGATCCAGACAGACCCTGAAGAACTTCTGATTATTAAGACCAGGCAATCACTTCTGGGATCCCTGACAGATCATGTTAAGGCAAATCACCCTTACGAGTAAGCCTAGCATTCCTTCTTTGTCTTTGTTTCTGTTTTTTTGTTTTTCATTTTCAACATTGGGGGGAAAATGGTGTGGAAGGGCAAATAGACTTGTAAATTGTGAACTGTTTAACTTATAAGCTAGACATTTAGTGTCTATTTTATAGTAGTTTTTGTTTTGTTTCTAAAAATCATATTGATTTACAAATATTTTTCTTTGTTTATTAATCTACTTTCAAGAATGTTTTTCAAATCTTAGCCAGAATATATTAAACTAGTAAAAAATAGTTTTAAACTTTTTGTTTTTGATTTTTGAAATATGGTTAAATTTGTTTATAGTATTTTTAAACAGTTGAAAACTAATTAAACAAATGAGTGGCATAAGTAGCAAGAAACCAGAAAAATAAGATCATAATCAAATGGAATCTTAGTTTCTCGTGGAACTCGTTATGGTGTTATCACACTTTCCAGAGAGTATAGGGGAATCAGGGAGTTGTTTTAAAATTCATATTTTGTCCCCTCTAAGGATTTACCTCCGTTTTTAATACAGGGTGCCTGAAGTTATTGCTTTGCGTATAACTGGTGGCAGCCTCGAGTACCTAGATTGGATCAAAAGCAGCACGAAGGAC

mRNA sequence

ATGGCCTTCACTCTCTGCAGCAGAGTCTCGGCTCCACTGCTATCCTCCTCTGCCCTACGACGTCGTTTGCCCCTTGTTGGCGCGTTCTGCGTACTGAGCTTCGGAATTTCCCATATCTTCATCTCCAGAACGGGTTCCGCTCTGTCTCTGCCTTTTGCCCCTCTCTTGAGATCGAAGTTTGGTGGTCAAGGACCAGCTAGGAACGTACATTTTGTAAGAATGGAAGCGACTAGCGCCGCGGTGCCTAGCATTGTTGTTTATGTCACCGTCCCGAATAGGGAAGCAGGTAAGAAATTGGCTGAAAGCATTGTCAAAGAAAAGCTTGCTGCTTGTGTTAACATAGTACCTGGTATAGAATCTGTCTACCAGTGGAAGGGAGAGATCCAGACAGACCCTGAAGAACTTCTGATTATTAAGACCAGGCAATCACTTCTGGGATCCCTGACAGATCATGTTAAGGCAAATCACCCTTACGAGGTGCCTGAAGTTATTGCTTTGCGTATAACTGGTGGCAGCCTCGAGTACCTAGATTGGATCAAAAGCAGCACGAAGGAC

Coding sequence (CDS)

ATGGCCTTCACTCTCTGCAGCAGAGTCTCGGCTCCACTGCTATCCTCCTCTGCCCTACGACGTCGTTTGCCCCTTGTTGGCGCGTTCTGCGTACTGAGCTTCGGAATTTCCCATATCTTCATCTCCAGAACGGGTTCCGCTCTGTCTCTGCCTTTTGCCCCTCTCTTGAGATCGAAGTTTGGTGGTCAAGGACCAGCTAGGAACGTACATTTTGTAAGAATGGAAGCGACTAGCGCCGCGGTGCCTAGCATTGTTGTTTATGTCACCGTCCCGAATAGGGAAGCAGGTAAGAAATTGGCTGAAAGCATTGTCAAAGAAAAGCTTGCTGCTTGTGTTAACATAGTACCTGGTATAGAATCTGTCTACCAGTGGAAGGGAGAGATCCAGACAGACCCTGAAGAACTTCTGATTATTAAGACCAGGCAATCACTTCTGGGATCCCTGACAGATCATGTTAAGGCAAATCACCCTTACGAGGTGCCTGAAGTTATTGCTTTGCGTATAACTGGTGGCAGCCTCGAGTACCTAGATTGGATCAAAAGCAGCACGAAGGAC

Protein sequence

MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKFGGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIESVYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIKSSTKD

Homology

[Close]

BLAST of MC08g0235 vs. ExPASy Swiss-Prot
Match: P93009 (Protein CutA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CUTA PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 8.3e-54
Identity = 118/189 (62.43%), Postives = 145/189 (76.72%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLS-FGISHIFIS---RTGSALSLPFAPLL 60
           MA +L +R+SA + S    RR  P+VGAFCVLS   IS +  S   ++G A S    PLL
Sbjct: 1   MASSLTTRLSAVIGS----RRSFPIVGAFCVLSTLSISSLSSSSPFKSGCAQSFSVVPLL 60

Query: 61  RSKFGGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP 120
           RSKF  +  + +   +RME +S  VPSIVVYVTVPNREAGKKLA SIV+EKLAACVNIVP
Sbjct: 61  RSKFSSKAFSSS---IRMEESSKTVPSIVVYVTVPNREAGKKLANSIVQEKLAACVNIVP 120

Query: 121 GIESVYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYL 180
           GIESVY+W+G++Q+D EELLIIKTRQSLL  LT+HV ANH Y+VPEVIAL ITGGS +YL
Sbjct: 121 GIESVYEWEGKVQSDSEELLIIKTRQSLLEPLTEHVNANHEYDVPEVIALPITGGSDKYL 180

Query: 181 DWIKSSTKD 186
           +W+K+ST++
Sbjct: 181 EWLKNSTRN 182

[Close]

BLAST of MC08g0235 vs. ExPASy Swiss-Prot
Match: Q109R6 (Protein CutA 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CUTA1 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.7e-46
Identity = 99/172 (57.56%), Postives = 119/172 (69.19%), Query Frame = 0


Query: 14  LSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKFGGQGPARNVHFVR 73
           LS   LRRR P+ GA   LS G                        F G     +    R
Sbjct: 28  LSPLLLRRRAPIAGALLFLSLG-----------------------AFAGVRSLSSSASAR 87

Query: 74  MEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIESVYQWKGEIQTDPE 133
           ME+TS  VPSIVVYVTVPN+EAGK+LA SI+ EKLAACVNIVPGIESVY W+G++QTD E
Sbjct: 88  MESTSTTVPSIVVYVTVPNKEAGKRLAGSIISEKLAACVNIVPGIESVYWWEGKVQTDAE 147

Query: 134 ELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIKSSTKD 186
           ELLIIKTR+SLL +LT+HVKANH Y+VPEVIAL I GG+L+YL+W+K+ST++
Sbjct: 148 ELLIIKTRESLLDALTEHVKANHEYDVPEVIALPIKGGNLKYLEWLKNSTRE 176

[Close]

BLAST of MC08g0235 vs. ExPASy Swiss-Prot
Match: Q7SIA8 (Divalent-cation tolerance protein CutA OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=cutA PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 4.8e-25
Identity = 50/99 (50.51%), Postives = 73/99 (73.74%), Query Frame = 0


Query: 85  VVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIESVYQWKGEIQTDPEELLIIKTRQSL 144
           VV +TVP+ E  + +A+++V+E+LAACVNIVPG+ S+Y+W+GE+  D E LL++KT    
Sbjct: 4   VVLITVPSEEVARTIAKALVEERLAACVNIVPGLTSIYRWQGEVVEDQELLLLVKTTTHA 63

Query: 145 LGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIKSST 184
              L + VKA HPY VPE++AL I  G+ EYLDW++ +T
Sbjct: 64  FPKLKERVKALHPYTVPEIVALPIAEGNREYLDWLRENT 102

[Close]

BLAST of MC08g0235 vs. ExPASy Swiss-Prot
Match: O60888 (Protein CutA OS=Homo sapiens OX=9606 GN=CUTA PE=1 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 52/112 (46.43%), Postives = 81/112 (72.32%), Query Frame = 0


Query: 75  EATSAAVPSIV--VYVTVPNREAGKKLAESIVKEKLAACVNIVPGIESVYQWKGEIQTDP 134
           ++ S  VP  V   +VT PN +  K++A ++V+++LAACVN++P I S+Y+WKG+I+ D 
Sbjct: 58  DSGSGYVPGSVSAAFVTCPNEKVAKEIARAVVEKRLAACVNLIPQITSIYEWKGKIEEDS 117

Query: 135 EELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIKSSTK 185
           E L++IKT+ SL+ +LTD V++ HPYEV EVIAL +  G+  YL W++  T+
Sbjct: 118 EVLMMIKTQSSLVPALTDFVRSVHPYEVAEVIALPVEQGNFPYLQWVRQVTE 169

[Close]

BLAST of MC08g0235 vs. ExPASy Swiss-Prot
Match: Q6MGD0 (Protein CutA OS=Rattus norvegicus OX=10116 GN=Cuta PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 2.0e-23
Identity = 64/160 (40.00%), Postives = 96/160 (60.00%), Query Frame = 0


Query: 27  GAFCVLSFGISHIFISRTGSALSLPFAPLLRSKFGGQGPARNVHFVRMEATSAAVPSIV- 86
           GA  +LSF      +      L LP A  L S   G  P++        + S  VP  V 
Sbjct: 14  GATLLLSFLCMPALLPVASRLLLLPRA--LLSMASGSPPSQ----PSPASGSGYVPGSVS 73

Query: 87  -VYVTVPNREAGKKLAESIVKEKLAACVNIVPGIESVYQWKGEIQTDPEELLIIKTRQSL 146
             +VT PN +  K++A ++V+++LAACVN++P I S+Y+WKG+I+ D E L++IKT+ SL
Sbjct: 74  AAFVTCPNEKVAKEIARAVVEKRLAACVNLIPQITSIYEWKGKIEEDSEVLMMIKTQSSL 133

Query: 147 LGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIKSSTK 185
           + +LT+ V++ HPYEV EVIAL +  G+  YL W+   T+
Sbjct: 134 VPALTEFVRSVHPYEVAEVIALPVEQGNPPYLHWVHQVTE 167

[Close]

BLAST of MC08g0235 vs. NCBI nr
Match: XP_022132074.1 (protein CutA, chloroplastic [Momordica charantia])

HSP 1 Score: 359 bits (921), Expect = 8.52e-125
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF
Sbjct: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
           GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK
Sbjct: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. NCBI nr
Match: XP_022997267.1 (protein CutA, chloroplastic [Cucurbita maxima])

HSP 1 Score: 324 bits (830), Expect = 6.36e-111
Identity = 164/185 (88.65%), Postives = 173/185 (93.51%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTLCSRV+ PLLS S LRRRLPLVGAFCVLSFG S++F+SRTGSALSLPFAPLLRS F
Sbjct: 1   MAFTLCSRVATPLLSPSVLRRRLPLVGAFCVLSFGFSNLFVSRTGSALSLPFAPLLRSTF 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
           G QGPARNVH ++ME  SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  GSQGPARNVHLIKMEGNSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQTD EELLIIKTRQSLLG+LTDHVKANHPYEVPEVIAL I GGS+EYL+WIK
Sbjct: 121 VYQWKGEIQTDAEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSVEYLEWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. NCBI nr
Match: XP_023545579.1 (protein CutA, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 323 bits (828), Expect = 1.28e-110
Identity = 163/185 (88.11%), Postives = 173/185 (93.51%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTLCSRV+ PLLS S LRRRLPLVGAFCVLSFG S++F+S+TGSALSLPFAPLLRS F
Sbjct: 1   MAFTLCSRVATPLLSPSVLRRRLPLVGAFCVLSFGFSNLFVSKTGSALSLPFAPLLRSTF 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
           G QGPARNVH ++ME  SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  GSQGPARNVHLIKMEGNSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQTD EELLIIKTRQSLLG+LTDHVKANHPYEVPEVIAL I GGS+EYL+WIK
Sbjct: 121 VYQWKGEIQTDAEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSMEYLEWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. NCBI nr
Match: KAG7029661.1 (Protein CutA, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 322 bits (824), Expect = 5.23e-110
Identity = 164/185 (88.65%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTLCSRV+ PLLSSS LRRRLPLVGAFCVLS G S++F+SRTGSALSLPFAPLLRS F
Sbjct: 1   MAFTLCSRVATPLLSSSVLRRRLPLVGAFCVLSLGFSNLFVSRTGSALSLPFAPLLRSTF 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
           G QGPARNVH ++ME  SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  GSQGPARNVHLIKMEGNSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQTD EELLIIKTRQSLLG+LTDHVKANHPYEVPEVIAL I GGS EYL+WIK
Sbjct: 121 VYQWKGEIQTDAEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSGEYLEWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. NCBI nr
Match: XP_038886215.1 (protein CutA, chloroplastic [Benincasa hispida] >XP_038886216.1 protein CutA, chloroplastic [Benincasa hispida])

HSP 1 Score: 321 bits (823), Expect = 7.42e-110
Identity = 161/185 (87.03%), Postives = 173/185 (93.51%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MA T CSR ++PL+SS  LRRRLPLVGAFCVLSFGIS++F+S+TGSALSLPF PLLRSKF
Sbjct: 1   MALTFCSRATSPLISSFVLRRRLPLVGAFCVLSFGISNLFVSKTGSALSLPFVPLLRSKF 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
             QGPARNVH ++ME +SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  ASQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQTDPEELLIIKTRQSLLG+LTDHVKANHPYEVPEVIAL I GGSLEYL+WIK
Sbjct: 121 VYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSLEYLEWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. ExPASy TrEMBL
Match: A0A6J1BS17 (protein CutA, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005035 PE=3 SV=1)

HSP 1 Score: 359 bits (921), Expect = 4.12e-125
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF
Sbjct: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
           GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK
Sbjct: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. ExPASy TrEMBL
Match: A0A6J1K4I7 (protein CutA, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111492224 PE=3 SV=1)

HSP 1 Score: 324 bits (830), Expect = 3.08e-111
Identity = 164/185 (88.65%), Postives = 173/185 (93.51%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTLCSRV+ PLLS S LRRRLPLVGAFCVLSFG S++F+SRTGSALSLPFAPLLRS F
Sbjct: 1   MAFTLCSRVATPLLSPSVLRRRLPLVGAFCVLSFGFSNLFVSRTGSALSLPFAPLLRSTF 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
           G QGPARNVH ++ME  SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  GSQGPARNVHLIKMEGNSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQTD EELLIIKTRQSLLG+LTDHVKANHPYEVPEVIAL I GGS+EYL+WIK
Sbjct: 121 VYQWKGEIQTDAEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSVEYLEWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. ExPASy TrEMBL
Match: A0A0A0LRU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370360 PE=3 SV=1)

HSP 1 Score: 320 bits (819), Expect = 1.46e-109
Identity = 161/185 (87.03%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTL SR + PL++S ALRRRLPLVGAFCVLSFGIS++F+S+TGSALSLPF PLLRSK 
Sbjct: 1   MAFTLSSRAATPLITSFALRRRLPLVGAFCVLSFGISNLFVSKTGSALSLPFVPLLRSKL 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
            GQGPARNVH ++ME +SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQ+DPEELLIIKTRQSLLG+LTDHVKANHPYEVPEVIAL I GGSLEYL+WIK
Sbjct: 121 VYQWKGEIQSDPEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSLEYLEWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. ExPASy TrEMBL
Match: A0A1S3BBF3 (protein CutA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488107 PE=3 SV=1)

HSP 1 Score: 319 bits (817), Expect = 2.95e-109
Identity = 160/185 (86.49%), Postives = 173/185 (93.51%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTL SR + PL+S  ALRRRLPLVGAFCVLSFGIS++F+S+TGSALS+PF PLLRSK 
Sbjct: 1   MAFTLSSRAATPLISCFALRRRLPLVGAFCVLSFGISNLFVSKTGSALSVPFVPLLRSKL 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
            GQGPARNVH ++ME +SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES
Sbjct: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           +YQWKGEIQTDPEELLIIKTRQSLLG+LTDHVKANHPYEVPEVIAL I GGSLEYL+WIK
Sbjct: 121 IYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSLEYLEWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. ExPASy TrEMBL
Match: A0A6J1HF76 (protein CutA, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111462991 PE=3 SV=1)

HSP 1 Score: 318 bits (816), Expect = 4.19e-109
Identity = 163/185 (88.11%), Postives = 171/185 (92.43%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60
           MAFTLCSRV+ PLLSSS LRRRLPLVGAFCVLS G S++F+SRTGSALSLPFAPLLRS F
Sbjct: 1   MAFTLCSRVATPLLSSSVLRRRLPLVGAFCVLSLGFSNLFVSRTGSALSLPFAPLLRSTF 60

Query: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGIES 120
           G QGPARNVH ++ME  SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIV GIES
Sbjct: 61  GSQGPARNVHLIKMEGNSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVSGIES 120

Query: 121 VYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYLDWIK 180
           VYQWKGEIQTD EELLIIKTRQSLLG+LTDHVKANHPYEVPEVIAL I GGS EYL+WIK
Sbjct: 121 VYQWKGEIQTDAEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSGEYLEWIK 180

Query: 181 SSTKD 185
           SSTKD
Sbjct: 181 SSTKD 185

[Close]

BLAST of MC08g0235 vs. TAIR 10
Match: AT2G33740.2 (Nitrogen regulatory PII-like, alpha/beta )

HSP 1 Score: 211.5 bits (537), Expect = 5.9e-55
Identity = 118/189 (62.43%), Postives = 145/189 (76.72%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLS-FGISHIFIS---RTGSALSLPFAPLL 60
           MA +L +R+SA + S    RR  P+VGAFCVLS   IS +  S   ++G A S    PLL
Sbjct: 1   MASSLTTRLSAVIGS----RRSFPIVGAFCVLSTLSISSLSSSSPFKSGCAQSFSVVPLL 60

Query: 61  RSKFGGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP 120
           RSKF  +  + +   +RME +S  VPSIVVYVTVPNREAGKKLA SIV+EKLAACVNIVP
Sbjct: 61  RSKFSSKAFSSS---IRMEESSKTVPSIVVYVTVPNREAGKKLANSIVQEKLAACVNIVP 120

Query: 121 GIESVYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEVPEVIALRITGGSLEYL 180
           GIESVY+W+G++Q+D EELLIIKTRQSLL  LT+HV ANH Y+VPEVIAL ITGGS +YL
Sbjct: 121 GIESVYEWEGKVQSDSEELLIIKTRQSLLEPLTEHVNANHEYDVPEVIALPITGGSDKYL 180

Query: 181 DWIKSSTKD 186
           +W+K+ST++
Sbjct: 181 EWLKNSTRN 182

[Close]

BLAST of MC08g0235 vs. TAIR 10
Match: AT2G33740.1 (Nitrogen regulatory PII-like, alpha/beta )

HSP 1 Score: 174.1 bits (440), Expect = 1.0e-43
Identity = 101/163 (61.96%), Postives = 121/163 (74.23%), Query Frame = 0


Query: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLS-FGISHIFIS---RTGSALSLPFAPLL 60
           MA +L +R+SA + S    RR  P+VGAFCVLS   IS +  S   ++G A S    PLL
Sbjct: 1   MASSLTTRLSAVIGS----RRSFPIVGAFCVLSTLSISSLSSSSPFKSGCAQSFSVVPLL 60

Query: 61  RSKFGGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP 120
           RSKF  +  + +   +RME +S  VPSIVVYVTVPNREAGKKLA SIV+EKLAACVNIVP
Sbjct: 61  RSKFSSKAFSSS---IRMEESSKTVPSIVVYVTVPNREAGKKLANSIVQEKLAACVNIVP 120

Query: 121 GIESVYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYE 160
           GIESVY+W+G++Q+D EELLIIKTRQSLL  LT+HV ANH YE
Sbjct: 121 GIESVYEWEGKVQSDSEELLIIKTRQSLLEPLTEHVNANHEYE 156

The following BLAST results are available for this feature:

BLAST of MC08g0235 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93009	8.3e-54	62.43	Protein CutA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CUTA PE=1 SV=1	[more]
Q109R6	3.7e-46	57.56	Protein CutA 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CUTA1 ...	[more]
Q7SIA8	4.8e-25	50.51	Divalent-cation tolerance protein CutA OS=Thermus thermophilus (strain ATCC 2763...	[more]
O60888	1.1e-24	46.43	Protein CutA OS=Homo sapiens OX=9606 GN=CUTA PE=1 SV=2	[more]
Q6MGD0	2.0e-23	40.00	Protein CutA OS=Rattus norvegicus OX=10116 GN=Cuta PE=1 SV=2	[more]

BLAST of MC08g0235 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132074.1	8.52e-125	100.00	protein CutA, chloroplastic [Momordica charantia]	[more]
XP_022997267.1	6.36e-111	88.65	protein CutA, chloroplastic [Cucurbita maxima]	[more]
XP_023545579.1	1.28e-110	88.11	protein CutA, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
KAG7029661.1	5.23e-110	88.65	Protein CutA, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038886215.1	7.42e-110	87.03	protein CutA, chloroplastic [Benincasa hispida] >XP_038886216.1 protein CutA, ch...	[more]

BLAST of MC08g0235 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BS17	4.12e-125	100.00	protein CutA, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005035 PE=3 ...	[more]
A0A6J1K4I7	3.08e-111	88.65	protein CutA, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111492224 PE=3 SV=...	[more]
A0A0A0LRU0	1.46e-109	87.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370360 PE=3 SV=1	[more]
A0A1S3BBF3	2.95e-109	86.49	protein CutA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488107 PE=3 SV=1	[more]
A0A6J1HF76	4.19e-109	88.11	protein CutA, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111462991 PE=3 S...	[more]

BLAST of MC08g0235 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G33740.2	5.9e-55	62.43	Nitrogen regulatory PII-like, alpha/beta	[more]
AT2G33740.1	1.0e-43	61.96	Nitrogen regulatory PII-like, alpha/beta	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015867	Nitrogen regulatory protein PII/ATP phosphoribosyltransferase, C-terminal	GENE3D	3.30.70.120		coord: 64..184 e-value: 2.0E-48 score: 164.9
IPR004323	Divalent ion tolerance protein, CutA	PFAM	PF03091	CutA1	coord: 84..181 e-value: 1.4E-37 score: 127.5
IPR004323	Divalent ion tolerance protein, CutA	PANTHER	PTHR23419	DIVALENT CATION TOLERANCE CUTA-RELATED	coord: 10..183
None	No IPR available	PANTHER	PTHR23419:SF8	FI09726P	coord: 10..183
IPR011322	Nitrogen regulatory PII-like, alpha/beta	SUPERFAMILY	54913	GlnB-like	coord: 83..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0235.1	MC08g0235.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010038	response to metal ion
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005507	copper ion binding