MC08g0226 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g0226 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 108.2 bits (269), Expect = 1.2e-22
Identity = 68/203 (33.50%), Postives = 98/203 (48.28%), Query Frame = 0

Query: 15  KFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSE------------VE 74
           K LCLHG+  SG +  K++G     +    +LHFP GP  +   ++            + 
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 75  GIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQ 134
           G  +   F WF+       Y ++DE L  I  YM + GPFDGL+GFSQGA + A L  + 
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQML 125

Query: 135 EKGV---ALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMP--AG 194
           + G       + P  KFV+ V G +   P   +  Y+P  T PSLH  G  D L+P    
Sbjct: 126 QPGQPPNPYVQHPPFKFVVFVGGFRAEKPEF-DHFYNPKLTTPSLHIAGTSDTLVPLARS 185

Query: 195 LELLESFVDPLVINHPKGHTVPR 201
            +L+E   +  V+ HP  H VP+
Sbjct: 186 KQLVERCENAHVLLHPGQHIVPQ 207

BLAST of MC08g0226 vs. ExPASy Swiss-Prot
Match: Q18169 (Esterase C25G4.2 OS=Caenorhabditis elegans OX=6239 GN=C25G4.2 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-16
Identity = 68/221 (30.77%), Postives = 103/221 (46.61%), Query Frame = 0

Query: 11  QRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEGIFDPPFF 70
           Q K + LCLHG+R   +  +++ G     V  L +  F +G    A    V+   D    
Sbjct: 4   QPKLRILCLHGYRQCDQSFRQKTGSTRKLVKSLAEFEFVNGVHSVA----VDEHVDSSRA 63

Query: 71  EWFQ-------FSADMTEY-INFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQ 130
            WF         S + TE  + F+E ++ +  ++ +NGPFDGLLGFSQGA +   L    
Sbjct: 64  WWFSNNEAMSFSSRESTEVAVGFEESVAAVVKFIEENGPFDGLLGFSQGASMVHLLIAKA 123

Query: 131 EKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLM--PAGLEL 190
           + G    K+P I+F I  SG   LS              PS+H  G+ D ++  P   ++
Sbjct: 124 QLGE--IKLPGIRFAIFFSGFLSLSSKHDSLTLLRIKEFPSMHVFGDADEIVARPKSEKM 183

Query: 191 LESF-VDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 221
            + F V+PL I H  GH VP + +   KI     E++ + I
Sbjct: 184 ADMFDVEPLRIAHDGGHVVPSMSKHKEKIAGFMREQLDRKI 218

BLAST of MC08g0226 vs. ExPASy Swiss-Prot
Match: Q61YZ4 (Esterase CBG03338 OS=Caenorhabditis briggsae OX=6238 GN=CBG03338 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 6.3e-16
Identity = 64/219 (29.22%), Postives = 100/219 (45.66%), Query Frame = 0

Query: 11  QRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEGIFDPPFF 70
           Q K + LCLHG+R   +  +++ G     V  L D  F +G    A     E        
Sbjct: 4   QPKLRILCLHGYRQCDQSFRQKTGSTRKLVKALADFEFVNGIHSVAVDEHSE-----TSR 63

Query: 71  EWFQFSADMTEY---------INFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGL 130
            W+  +AD   +         + FDE ++ +  ++  NGPFDGLLGFSQGA +   L   
Sbjct: 64  AWWFSNADQMSFSSREPTDVSVGFDESVNAVVKFIEDNGPFDGLLGFSQGASMVHLLIAK 123

Query: 131 QEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLM--PAGLE 190
            + G    K+P I+F I  SG   LS              PS+H  G+ D ++  P   +
Sbjct: 124 AQLGE--IKLPGIRFAIFFSGFLSLSSKHDTLTSLRIKDFPSMHVFGDSDEIVARPKSEK 183

Query: 191 LLESF-VDPLVINHPKGHTVPRLDEKSLKIMESFIEKIS 218
           L + F ++P+ I H  GH VP + +   +I +   E+++
Sbjct: 184 LADQFDMEPIRIAHEGGHLVPSMSKHKERITQFLREQMA 215

BLAST of MC08g0226 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-15
Identity = 66/222 (29.73%), Postives = 98/222 (44.14%), Query Frame = 0

Query: 11  QRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPF----------PSAGRSE 70
           +RK + LCLHGFR +    K + G     + ++ +L F D P           P +G   
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 71  ------VEGIFDPPF----------FEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDG 130
                 V   FD P           F+  Q+    T+   FD+ L++++    + GPFDG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQY---QTQTEGFDKSLTYLKTAFEEKGPFDG 470

Query: 131 LLGFSQGAVLSAALPGLQEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSL 190
           +LGFSQGA ++AA+ G QE+   L      +F ++ SG  F    L E     S  CPSL
Sbjct: 471 ILGFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSL 530

Query: 191 HFLG-----EEDFLMPAGLELLESFVD--PLVINHPKGHTVP 200
           H  G     +   +  A  +L   F D    ++ H  GH +P
Sbjct: 531 HIFGSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

BLAST of MC08g0226 vs. ExPASy Swiss-Prot
Match: O13897 (Uncharacterized hydrolase C22A12.06c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22A12.06c PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-15
Identity = 62/207 (29.95%), Postives = 99/207 (47.83%), Query Frame = 0

Query: 13  KPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFP-SAGRSEVEGI-FD---- 72
           K K LC+HG+  SGE+   ++      + D +D +FP GP      + E+ G  FD    
Sbjct: 6   KSKILCIHGYAESGELFSVKLRALRERMADSVDFYFPTGPIELDKAKDELNGSGFDALST 65

Query: 73  -----PPFFE--WFQFS--ADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAA 132
                P      W++ +  AD T+ +   +   ++  Y+ ++GPFDG+LGFSQG  L+A 
Sbjct: 66  VFSSSPASHRRGWWRINEYAD-TKQLEPTKAFEYLASYIKEHGPFDGILGFSQGTNLAAN 125

Query: 133 LPGLQ--EKGVALTKVPKIKFVIIVSGAKFLSPLLAEKA-YSPSFTCPSLHFLGEEDFLM 192
           L  L    K       P  +F +  SG  +  PLL + A ++     P+LH LG+ D ++
Sbjct: 126 LAALVTIPKYQEYFSQPPFRFALFFSG--YFRPLLMDGAVHATKLDLPTLHLLGKYDTVL 185

Query: 193 --PAGLELLESFVDPLVINHPKGHTVP 200
                  L+ +  D  V+ HP  H +P
Sbjct: 186 STETSTTLVRACKDAQVLFHPAAHQIP 209

BLAST of MC08g0226 vs. NCBI nr
Match: XP_022131301.1 (dihydrofolate reductase-like [Momordica charantia])

HSP 1 Score: 443 bits (1139), Expect = 7.12e-157
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0

Query: 4   MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG 63
           MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG
Sbjct: 1   MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG 60

Query: 64  IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE 123
           IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE
Sbjct: 61  IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE 120

Query: 124 KGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLES 183
           KGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLES
Sbjct: 121 KGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLES 180

Query: 184 FVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           FVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI
Sbjct: 181 FVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 217

BLAST of MC08g0226 vs. NCBI nr
Match: XP_022131300.1 (dihydrofolate reductase-like [Momordica charantia])

HSP 1 Score: 386 bits (991), Expect = 9.35e-134
Identity = 182/219 (83.11%), Postives = 202/219 (92.24%), Query Frame = 0

Query: 2   SAMGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEV 61
           S MGSE +IQRKP+FLCLHGFRTSG+ILKKQVGKWPSSVLD  DLHF D PFP+ G+S+V
Sbjct: 33  SIMGSEAKIQRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDV 92

Query: 62  EGIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGL 121
           EGIFDPP+FEWFQFSADM EY NFDECLSFIEDYMLK+GPFDG LGFSQGA+LSAALPGL
Sbjct: 93  EGIFDPPYFEWFQFSADMMEYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGL 152

Query: 122 QEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELL 181
           QEKG+ALTKVPKIKFV+IVSGAKF SP LAEKAYSPS  CPSLHFLG+EDFLMP GL+LL
Sbjct: 153 QEKGIALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLL 212

Query: 182 ESFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           ES+++PLVINHPKGHTVPRLDEK LK+MESFIE+IS+ +
Sbjct: 213 ESYIEPLVINHPKGHTVPRLDEKGLKVMESFIERISQTL 251

BLAST of MC08g0226 vs. NCBI nr
Match: KAG6598716.1 (hypothetical protein SDJN03_08494, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 365 bits (936), Expect = 6.70e-126
Identity = 169/217 (77.88%), Postives = 196/217 (90.32%), Query Frame = 0

Query: 4   MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG 63
           MG++GEI+RKP+FLCLHGFRTSG ILKKQVGKWP SVL+ LDL F D PFP+ G+S++EG
Sbjct: 1   MGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEG 60

Query: 64  IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE 123
           IFDPP+FEWFQFS + TEY NFDECLSFIEDYMLK+GPFDG LGFSQGA+LSAALPGLQ 
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQA 120

Query: 124 KGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLES 183
           KG+ALTKVPKIKFVIIVSGAKF S  +AEKAYSP   CPSLHFLG+EDFLMP G +LLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLGKEDFLMPHGQKLLES 180

Query: 184 FVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           ++DP++INHPKGHT+PRLD++ L++MESFIEKISKM+
Sbjct: 181 YIDPVIINHPKGHTIPRLDDEGLEVMESFIEKISKML 217

BLAST of MC08g0226 vs. NCBI nr
Match: XP_023545459.1 (dihydrofolate reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 364 bits (934), Expect = 3.51e-125
Identity = 170/220 (77.27%), Postives = 197/220 (89.55%), Query Frame = 0

Query: 1   ISAMGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSE 60
           I  MG++GEI+RKP+FLCLHGFRTSG ILKKQVGKWP SVL+ LDL F D PFP+ G+S+
Sbjct: 25  IPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSD 84

Query: 61  VEGIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPG 120
           +EGIFDPP+FEWFQFS + TEY NFDECLSFIEDYMLK+GPFDG LGFSQGA+LSAALPG
Sbjct: 85  IEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPG 144

Query: 121 LQEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLEL 180
           LQ KG+ALTKVPKIKFVIIVSGAKF S  +AEKAYS +  CPSLHFLG+EDFLMP G +L
Sbjct: 145 LQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFLMPHGQKL 204

Query: 181 LESFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           LES+++P+VINHPKGHT+PRLD+K L++MESFIEKISKM+
Sbjct: 205 LESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKML 244

BLAST of MC08g0226 vs. NCBI nr
Match: XP_022961839.1 (dihydrofolate reductase-like [Cucurbita moschata])

HSP 1 Score: 364 bits (934), Expect = 3.51e-125
Identity = 169/220 (76.82%), Postives = 197/220 (89.55%), Query Frame = 0

Query: 1   ISAMGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSE 60
           I  MG++GEI+RKP+FLCLHGFRTSG ILKKQVGKWP SVL+ LDL F D PFP+ G+S+
Sbjct: 25  IPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSD 84

Query: 61  VEGIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPG 120
           +EGIFDPP+FEWFQFS + TEY NFDECLSFIEDYMLK+GPFDG LGFSQGA+LSAALPG
Sbjct: 85  IEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPG 144

Query: 121 LQEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLEL 180
           LQ KG+ALTKVPKIKFVIIVSGAKF S  +AEKAYSP   CPSLHFLG+EDFLMP G +L
Sbjct: 145 LQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLGKEDFLMPHGQKL 204

Query: 181 LESFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           LES+++P++INHPKGHT+PRLD++ L++MESFIEKISKM+
Sbjct: 205 LESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKISKML 244

BLAST of MC08g0226 vs. ExPASy TrEMBL
Match: A0A6J1BP70 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111004564 PE=4 SV=1)

HSP 1 Score: 443 bits (1139), Expect = 3.45e-157
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0

Query: 4   MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG 63
           MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG
Sbjct: 1   MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG 60

Query: 64  IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE 123
           IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE
Sbjct: 61  IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE 120

Query: 124 KGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLES 183
           KGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLES
Sbjct: 121 KGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLES 180

Query: 184 FVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           FVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI
Sbjct: 181 FVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 217

BLAST of MC08g0226 vs. ExPASy TrEMBL
Match: A0A6J1BPA7 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111004563 PE=4 SV=1)

HSP 1 Score: 386 bits (991), Expect = 4.53e-134
Identity = 182/219 (83.11%), Postives = 202/219 (92.24%), Query Frame = 0

Query: 2   SAMGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEV 61
           S MGSE +IQRKP+FLCLHGFRTSG+ILKKQVGKWPSSVLD  DLHF D PFP+ G+S+V
Sbjct: 33  SIMGSEAKIQRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDV 92

Query: 62  EGIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGL 121
           EGIFDPP+FEWFQFSADM EY NFDECLSFIEDYMLK+GPFDG LGFSQGA+LSAALPGL
Sbjct: 93  EGIFDPPYFEWFQFSADMMEYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGL 152

Query: 122 QEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELL 181
           QEKG+ALTKVPKIKFV+IVSGAKF SP LAEKAYSPS  CPSLHFLG+EDFLMP GL+LL
Sbjct: 153 QEKGIALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLL 212

Query: 182 ESFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           ES+++PLVINHPKGHTVPRLDEK LK+MESFIE+IS+ +
Sbjct: 213 ESYIEPLVINHPKGHTVPRLDEKGLKVMESFIERISQTL 251

BLAST of MC08g0226 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 364 bits (934), Expect = 1.70e-125
Identity = 169/220 (76.82%), Postives = 197/220 (89.55%), Query Frame = 0

Query: 1   ISAMGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSE 60
           I  MG++GEI+RKP+FLCLHGFRTSG ILKKQVGKWP SVL+ LDL F D PFP+ G+S+
Sbjct: 25  IPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSD 84

Query: 61  VEGIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPG 120
           +EGIFDPP+FEWFQFS + TEY NFDECLSFIEDYMLK+GPFDG LGFSQGA+LSAALPG
Sbjct: 85  IEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPG 144

Query: 121 LQEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLEL 180
           LQ KG+ALTKVPKIKFVIIVSGAKF S  +AEKAYSP   CPSLHFLG+EDFLMP G +L
Sbjct: 145 LQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLGKEDFLMPHGQKL 204

Query: 181 LESFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           LES+++P++INHPKGHT+PRLD++ L++MESFIEKISKM+
Sbjct: 205 LESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKISKML 244

BLAST of MC08g0226 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 362 bits (929), Expect = 9.80e-125
Identity = 169/220 (76.82%), Postives = 197/220 (89.55%), Query Frame = 0

Query: 1   ISAMGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSE 60
           I  MG++GEI+RKP+FLCLHGFRTSG ILKKQVGKWP SVL+ LDL F D PFP+ G+S+
Sbjct: 25  IPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSD 84

Query: 61  VEGIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPG 120
           +EGIFDPP+FEWFQFS + TEY NFDECLSFIEDYMLK+GPFDG LGFSQGA+LSAALPG
Sbjct: 85  IEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPG 144

Query: 121 LQEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLEL 180
           LQ KG+ALTKVPKIKFVIIVSGAKF S  +AEKAYS +  CPSLHFLG+EDFLMP G +L
Sbjct: 145 LQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFLMPHGQKL 204

Query: 181 LESFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           LES+++P+VINHPKGHT+PRLD++ L++MESFIEKISKM+
Sbjct: 205 LESYIEPVVINHPKGHTIPRLDDRGLEVMESFIEKISKML 244

BLAST of MC08g0226 vs. ExPASy TrEMBL
Match: A0A5A7VFL2 (Dihydrofolate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004510 PE=4 SV=1)

HSP 1 Score: 348 bits (893), Expect = 1.15e-119
Identity = 162/217 (74.65%), Postives = 190/217 (87.56%), Query Frame = 0

Query: 4   MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG 63
           MGS+ +I  KP+FLCLHGFRTS  ILKKQVGKWP SVLD LDLHF D PFP+ G+S+VEG
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 64  IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE 123
           IFDPP+FEWFQFS + TEY NFDECLSFIE+YM+++GPFDG LGFSQGA+LSAALPG Q 
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 124 KGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLES 183
           KG+ALTKVPKIKFVIIVSGAKF S  +AEKAYS    CPSLHFLGEEDFLMP G +LLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 184 FVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 220
           +++P +I HPKGHT+PRLD+K+L++MESFI++ISKM+
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKML 217

BLAST of MC08g0226 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 302.0 bits (772), Expect = 4.0e-82
Identity = 141/218 (64.68%), Postives = 174/218 (79.82%), Query Frame = 0

Query: 4   MGSEG-EIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVE 63
           MGSEG  I RKP+FLCLHGFRTSGEI+K Q+ KWP SV+D LDL F D PFP  G+S+VE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 64  GIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQ 123
           GIFDPP++EWFQF+ + TEY NF++CL ++ED M+K GPFDGL+GFSQGA+LS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 124 EKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLE 183
            KG+A  KVPKIKFVII+ GAK  S  LAE AYS S    SLHFLGE DFL P G +L+E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

Query: 184 SFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 221
           S+ +P+V++HPKGHTVPRLDEKSL+ + +FI+ +  ++
Sbjct: 181 SYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLV 218

BLAST of MC08g0226 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 8.3e-72
Identity = 127/225 (56.44%), Postives = 167/225 (74.22%), Query Frame = 0

Query: 2   SAMGSEGEIQRK-PKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSE 61
           +AM  E  ++ K P+ LCLHGFRTSG IL+  +GKWP ++L  LDL F D PFP+ G+S+
Sbjct: 17  TAMAPEIHLKPKNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSD 76

Query: 62  VEGIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPG 121
           VE  FDPP++EW+Q +    EY NF+ECL++IEDYM+KNGPFDGLLGFSQGA L+AA+PG
Sbjct: 77  VERFFDPPYYEWYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPG 136

Query: 122 LQEKGVALTKVPKIKFVIIVSGAK-----FLSPLLAEKAYSPSFTCPSLHFLGEEDFLMP 181
           +QE+G ALTKVPK+KF++I+SGAK     F  P  A  A+S    CPSLHF+GE DFL  
Sbjct: 137 MQEQGSALTKVPKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKI 196

Query: 182 AGLELLESFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKMI 221
            G  L+ESFV+P+VI+H  GH +P+LD K+ + M SF + I +M+
Sbjct: 197 EGEVLVESFVEPVVIHHSGGHIIPKLDTKAEETMLSFFQSIRQML 241

BLAST of MC08g0226 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 238.8 bits (608), Expect = 4.1e-63
Identity = 113/166 (68.07%), Postives = 132/166 (79.52%), Query Frame = 0

Query: 4   MGSEG-EIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVE 63
           MGSEG  I RKP+FLCLHGFRTSGEI+K Q+ KWP SV+D LDL F D PFP  G+S+VE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 64  GIFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQ 123
           GIFDPP++EWFQF+ + TEY NF++CL ++ED M+K GPFDGL+GFSQGA+LS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 124 EKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSLHFLG 169
            KG+A  KVPKIKFVII+ GAK  S  LAE AYS S    SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

BLAST of MC08g0226 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 84.7 bits (208), Expect = 9.9e-17
Identity = 66/222 (29.73%), Postives = 98/222 (44.14%), Query Frame = 0

Query: 11  QRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPF----------PSAGRSE 70
           +RK + LCLHGFR +    K + G     + ++ +L F D P           P +G   
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 71  ------VEGIFDPPF----------FEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDG 130
                 V   FD P           F+  Q+    T+   FD+ L++++    + GPFDG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQY---QTQTEGFDKSLTYLKTAFEEKGPFDG 470

Query: 131 LLGFSQGAVLSAALPGLQEKGVALTKVPKIKFVIIVSGAKFLSPLLAEKAYSPSFTCPSL 190
           +LGFSQGA ++AA+ G QE+   L      +F ++ SG  F    L E     S  CPSL
Sbjct: 471 ILGFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSL 530

Query: 191 HFLG-----EEDFLMPAGLELLESFVD--PLVINHPKGHTVP 200
           H  G     +   +  A  +L   F D    ++ H  GH +P
Sbjct: 531 HIFGSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564