MC08g0217 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0217
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	MC08: 1544688 .. 1545545 (+)
RNA-Seq Expression	MC08g0217
Synteny	MC08g0217

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTCACAATTAGCAGAAAACTCTTCCCTTCTACAACTAACTGTTCAGGCTGCCCCTACGACTGTCCTTTCGATTGCTACACTTTTCCAGACTTCGACTACTCGCCGCCGCCGCCGCCGCCTCACCAAAATCAAGCCCTTCAACCTTTCATCGTCGTCGTAATCGCTCTCTCCGTCGGGTTCTTCGCCGCTTTGAACTTCTACATGGTCGTCGTAAAGTGCCGTGGCCCCCGGAACTCGGGGGCGGCGGCCTCCCAATCCGACGGCGGAGAGGGAGAGTTACTGGACCGAAATCAAGTGGATCATCCAATTTGGTTCATCAACACCGTCGGCCTGCATCAATCCATCATCAATTCCATTGCGGCGTGCAAATTCAAGCAGGGGGAGGGTCTGATCGAAGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCGACAAGGCGAGATGCTTAGATTGCTGCCCAAATGTAGCCATGCCTTTCATATTGGATGTGTAGACACTTGGCTCAGGTCTCACACCACTTGCCCTCTCTGTCGAGCCCTTATACTCACGGATTTGGGTGCGAGGCCCCCCAATTTGGGTTCTGTGAATCAGATCGAGGCTAATTCGGGCGGAAATGAGGAGACCCAGATGGAGAATGGGGAGATTAATGGCGAAGTTGTGGGGGAAAATCAGGCTGTTGTTGCTGTTTCTGGTGGGAGCTTGGAGAACAGGGGAGAAGTTGTTGATGAACAGAGAGAAGTTGTTGTTCAGGTTGGGGAGATTGTTCCAAAAGAGGGGATTACTGAATCTGATCAGAATCATGGAGTTGAAGGAAATGAGATTCTGATGAATAATCAGATGGATTCTTCTTCT

mRNA sequence

ATGGCGCTCACAATTAGCAGAAAACTCTTCCCTTCTACAACTAACTGTTCAGGCTGCCCCTACGACTGTCCTTTCGATTGCTACACTTTTCCAGACTTCGACTACTCGCCGCCGCCGCCGCCGCCTCACCAAAATCAAGCCCTTCAACCTTTCATCGTCGTCGTAATCGCTCTCTCCGTCGGGTTCTTCGCCGCTTTGAACTTCTACATGGTCGTCGTAAAGTGCCGTGGCCCCCGGAACTCGGGGGCGGCGGCCTCCCAATCCGACGGCGGAGAGGGAGAGTTACTGGACCGAAATCAAGTGGATCATCCAATTTGGTTCATCAACACCGTCGGCCTGCATCAATCCATCATCAATTCCATTGCGGCGTGCAAATTCAAGCAGGGGGAGGGTCTGATCGAAGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCGACAAGGCGAGATGCTTAGATTGCTGCCCAAATGTAGCCATGCCTTTCATATTGGATGTGTAGACACTTGGCTCAGGTCTCACACCACTTGCCCTCTCTGTCGAGCCCTTATACTCACGGATTTGGGTGCGAGGCCCCCCAATTTGGGTTCTGTGAATCAGATCGAGGCTAATTCGGGCGGAAATGAGGAGACCCAGATGGAGAATGGGGAGATTAATGGCGAAGTTGTGGGGGAAAATCAGGCTGTTGTTGCTGTTTCTGGTGGGAGCTTGGAGAACAGGGGAGAAGTTGTTGATGAACAGAGAGAAGTTGTTGTTCAGGTTGGGGAGATTGTTCCAAAAGAGGGGATTACTGAATCTGATCAGAATCATGGAGTTGAAGGAAATGAGATTCTGATGAATAATCAGATGGATTCTTCTTCT

Coding sequence (CDS)

ATGGCGCTCACAATTAGCAGAAAACTCTTCCCTTCTACAACTAACTGTTCAGGCTGCCCCTACGACTGTCCTTTCGATTGCTACACTTTTCCAGACTTCGACTACTCGCCGCCGCCGCCGCCGCCTCACCAAAATCAAGCCCTTCAACCTTTCATCGTCGTCGTAATCGCTCTCTCCGTCGGGTTCTTCGCCGCTTTGAACTTCTACATGGTCGTCGTAAAGTGCCGTGGCCCCCGGAACTCGGGGGCGGCGGCCTCCCAATCCGACGGCGGAGAGGGAGAGTTACTGGACCGAAATCAAGTGGATCATCCAATTTGGTTCATCAACACCGTCGGCCTGCATCAATCCATCATCAATTCCATTGCGGCGTGCAAATTCAAGCAGGGGGAGGGTCTGATCGAAGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCGACAAGGCGAGATGCTTAGATTGCTGCCCAAATGTAGCCATGCCTTTCATATTGGATGTGTAGACACTTGGCTCAGGTCTCACACCACTTGCCCTCTCTGTCGAGCCCTTATACTCACGGATTTGGGTGCGAGGCCCCCCAATTTGGGTTCTGTGAATCAGATCGAGGCTAATTCGGGCGGAAATGAGGAGACCCAGATGGAGAATGGGGAGATTAATGGCGAAGTTGTGGGGGAAAATCAGGCTGTTGTTGCTGTTTCTGGTGGGAGCTTGGAGAACAGGGGAGAAGTTGTTGATGAACAGAGAGAAGTTGTTGTTCAGGTTGGGGAGATTGTTCCAAAAGAGGGGATTACTGAATCTGATCAGAATCATGGAGTTGAAGGAAATGAGATTCTGATGAATAATCAGATGGATTCTTCTTCT

Protein sequence

MALTISRKLFPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSVGFFAALNFYMVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSS

Homology

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BLAST of MC08g0217 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 164.1 bits (414), Expect = 2.4e-39
Identity = 107/263 (40.68%), Postives = 129/263 (49.05%), Query Frame = 0


Query: 1   MALTISRKLFPS-------TTNCSG--CPYDCPFDCYTFPD-FDYSPPPPP----PHQNQ 60
           MA    RKLFP+       T +CS   C   CP++CY  PD +  SP  PP    P  + 
Sbjct: 1   MARKKHRKLFPTLASETNKTLDCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSP 60

Query: 61  ALQPFI------------------------VVVIALSVGFFAALNFYMVVVKCRGPRNSG 120
              P I                         V+  L  GFF    F+   V      N G
Sbjct: 61  CPSPSISAVYLPSQDSSSSLDAISIITITGAVLAILLTGFFLVAKFFSDSV---NRVNQG 120

Query: 121 AAASQSDGG----EGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTEC 180
              S ++      E E  DR QVDHPIW I T GL QSIINSI  C +K+G+GLIE T+C
Sbjct: 121 TYQSDNEDNDTVMEEEFQDREQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDC 180

Query: 181 SVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPNLGSVN 222
            VCL+EF + E LRLLPKC+HAFHI C+DTWL SHT CPLCRA I       P   G V 
Sbjct: 181 PVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVE 240

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BLAST of MC08g0217 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.5e-35
Identity = 80/162 (49.38%), Postives = 103/162 (63.58%), Query Frame = 0


Query: 37  PPPPPPHQNQALQPFIVVVIALSVGFFAALNFYMVVVKCRGPRNSGAAASQSDGG----E 96
           PPPPPP +   L P  + V+  S+  F  L F++ +   +  R S A+ +  D      E
Sbjct: 30  PPPPPPRELSLLLPTSICVVG-SIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQEDE 89

Query: 97  GELLDRNQVD-HPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEML 156
            E  +R+  D H +W I TVGLH+S INSI    FK+GEG+I+GTECSVCL+EF + E L
Sbjct: 90  DETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEFEEDESL 149

Query: 157 RLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPN 194
           RLLPKCSHAFH+ C+DTWL SH  CPLCRA +L  L   PP+
Sbjct: 150 RLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVL--LITEPPH 188

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BLAST of MC08g0217 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 2.4e-28
Identity = 87/264 (32.95%), Postives = 120/264 (45.45%), Query Frame = 0


Query: 10  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPP--------PPHQNQALQPFIVVVIALS 69
           +  T +CS   C   CP  CY    F + PPPP                 P ++ +I + 
Sbjct: 11  YEPTRDCSQGLCSMYCPQWCY----FIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVL 70

Query: 70  VGFFAALNFYMVVVKCRGP----------RNSGAAASQSDGGEGELLDRNQV-DHPIWFI 129
              F  +++Y  + K  G            +SG AA     G G      Q   H  W +
Sbjct: 71  ASAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWNV 130

Query: 130 N-TVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVD 189
           +   GL +++IN I  CK+++G+G +  T+CSVCL EF  GE LRLLP+CSHAFH  C+D
Sbjct: 131 SPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCID 190

Query: 190 TWLRSHTTCPLCRA-LILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQ 249
           TWL+SH+ CPLCRA +    +G   P                      G   GE  G+N 
Sbjct: 191 TWLKSHSNCPLCRANITFVTVGLASPE-------------------PEGCAPGETGGDNT 248

Query: 250 AVVAVSGGSLENRGEVVDEQREVV 251
             V V    LEN   + +EQ+E V
Sbjct: 251 HEVVVVMDGLEN---LCEEQQEAV 248

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BLAST of MC08g0217 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.6e-27
Identity = 76/228 (33.33%), Postives = 118/228 (51.75%), Query Frame = 0


Query: 10  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPPP--------PHQNQALQPFIVVVIALS 69
           + S  +CS   C   CP  CY        PPPPP           + +  P ++ +I + 
Sbjct: 11  YDSYNDCSQGICNIYCPQWCYLI-----FPPPPPSFFLDDDSSSSSSSFSPLLIALIGIL 70

Query: 70  VGFFAALNFYMVVVK-C--RGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQS 129
                 +++Y ++ K C      +S    + +  GEG      ++       N  GL++S
Sbjct: 71  TSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRIS-----TNGDGLNES 130

Query: 130 IINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTC 189
           +I SI   K+K G+G ++G++CSVCLSEF + E LRLLPKC+HAFH+ C+DTWL+SH+ C
Sbjct: 131 MIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNC 190

Query: 190 PLCRALILTDLGARPPNLG-SVNQIEANSGGNEETQMENGEINGEVVG 224
           PLCRA + T +     ++G +V+ + AN   +        EIN  + G
Sbjct: 191 PLCRAFV-TGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAG 227

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BLAST of MC08g0217 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 3.7e-24
Identity = 77/247 (31.17%), Postives = 119/247 (48.18%), Query Frame = 0


Query: 10  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPP-------PPHQNQALQPFIVVVIALSV 69
           + S  +CS   C   CP  CY        PPPP           +    P ++ +I +  
Sbjct: 14  YDSYRDCSQGVCSVYCPQWCYVI-----FPPPPSFYLDDEDDSSSSDFSPLLIALIGILA 73

Query: 70  GFFAALNFYMVVVK-CRGPRNSGAAASQS-----------DGGEGELLDRNQVDHPIWFI 129
             F  +++Y ++ K C   R++ ++ S +            G      + N   +P   I
Sbjct: 74  SAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNN--NNNGATNPNQTI 133

Query: 130 ---NTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 189
                 GL +S+I SI   K+++ +G +E ++CSVCLSEF++ E LRLLPKC+HAFH+ C
Sbjct: 134 GGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPC 193

Query: 190 VDTWLRSHTTCPLCRALILTDLGARPPNLGSVNQIEAN---SGGNEET-----QMENGEI 225
           +DTWL+SH+ CPLCRA I+T       +L +   +  N   S G++        +EN   
Sbjct: 194 IDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNLDLENSRS 253

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BLAST of MC08g0217 vs. NCBI nr
Match: XP_022132045.1 (RING-H2 finger protein ATL54-like [Momordica charantia])

HSP 1 Score: 576 bits (1484), Expect = 3.13e-206
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0


Query: 1   MALTISRKLFPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSV 60
           MALTISRKLFPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSV
Sbjct: 52  MALTISRKLFPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSV 111

Query: 61  GFFAALNFYMVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINS 120
           GFFAALNFYMVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINS
Sbjct: 112 GFFAALNFYMVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINS 171

Query: 121 IAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCR 180
           IAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCR
Sbjct: 172 IAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCR 231

Query: 181 ALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRG 240
           ALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRG
Sbjct: 232 ALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRG 291

Query: 241 EVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSS 286
           EVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSS
Sbjct: 292 EVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSS 337

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BLAST of MC08g0217 vs. NCBI nr
Match: XP_038884462.1 (RING-H2 finger protein ATL54-like [Benincasa hispida])

HSP 1 Score: 369 bits (947), Expect = 2.12e-125
Identity = 201/305 (65.90%), Postives = 225/305 (73.77%), Query Frame = 0


Query: 1   MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 60
           MA+TISRKLFP        TTNCSGCPYDCPFDCY  PDF YS PPPPP    HQ Q   
Sbjct: 1   MAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQL-- 60

Query: 61  PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 120
           P +++++ L VGFF A +FY++VVKCR    G  N G  A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTLIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 180
           WFI T GL QS+INSI  CK+K+ EGLIEGTECSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRSHTTCPLCRALILTDLGA----RPPNLGSVNQIEANSGGNEETQMENGEINGEV 240
           VDTWLR+HTTCPLCRA ILTD  A    RPPNLG VNQ EAN G NEETQMEN EINGEV
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEV 240

Query: 241 VGENQAVVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQ 286
           + EN+ VV  S G+ ENRG+VVDEQREV           GI ++D +  VEGNE   NNQ
Sbjct: 241 LVENEGVV--SRGNFENRGDVVDEQREV-----------GIVQTDDSK-VEGNESTRNNQ 289

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BLAST of MC08g0217 vs. NCBI nr
Match: XP_008444871.1 (PREDICTED: RING-H2 finger protein ATL54-like [Cucumis melo] >KAA0065127.1 RING-H2 finger protein ATL54-like [Cucumis melo var. makuwa])

HSP 1 Score: 353 bits (905), Expect = 9.53e-119
Identity = 197/309 (63.75%), Postives = 223/309 (72.17%), Query Frame = 0


Query: 1   MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 60
           MA+T SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLFDHQKQL-- 60

Query: 61  PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 120
           P +++V+ L V FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 180
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRSHTTCPLCRALILTDL------GARPPNLGSVNQIEANSGGNEETQMENGEING 240
           VDTWLR+HTTCPLCRA ILTD         RPPNLG +NQ E N G NE+TQMEN  ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 241 EVVGENQA-VVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILM 286
           E V EN+  VV++S  S ENRG+VVDEQREV     EIV KEG  ESD +  VEGNE   
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQREVE----EIVQKEGNFESDDSK-VEGNESTR 300

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BLAST of MC08g0217 vs. NCBI nr
Match: XP_011649673.1 (RING-H2 finger protein ATL54 [Cucumis sativus] >KGN62717.1 hypothetical protein Csa_022380 [Cucumis sativus])

HSP 1 Score: 349 bits (896), Expect = 2.07e-117
Identity = 191/307 (62.21%), Postives = 221/307 (71.99%), Query Frame = 0


Query: 1   MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 60
           MA+T+SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTLSRKLFPVATGTALPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLSDHQKQL-- 60

Query: 61  PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 120
           P  ++++ L + FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTTIIILILFIAFFVAFTFYIIVVKCRSWYSGSANEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 180
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRSHTTCPLCRALILTDL----GARPPNLGSVNQIEANSGGNEETQMENGEINGEV 240
           VDTWLR+HTTCPLCRA ILTD       RPPN+G +NQ E N G NE+TQMEN   N E 
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTPNSVRPPNIGPLNQNEGNLGLNEDTQMENENTNREA 240

Query: 241 VGENQAV-VAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMN- 286
           V EN+   V++S  S ENRG+ VDEQREV     EIV KEG  ESD +  VEGNE + N 
Sbjct: 241 VRENEGGGVSISSESSENRGDAVDEQREVE----EIVQKEGNFESDDSK-VEGNESMRND 300

[Close]

BLAST of MC08g0217 vs. NCBI nr
Match: XP_022997232.1 (RING-H2 finger protein ATL54-like [Cucurbita maxima])

HSP 1 Score: 335 bits (859), Expect = 5.16e-112
Identity = 182/295 (61.69%), Postives = 213/295 (72.20%), Query Frame = 0


Query: 1   MALTISRKLFPSTT-------NCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIV 60
           MA+ I+RKLFP  T       NCSGCPYDCPFDC   PDF +SPPPP     + L   ++
Sbjct: 1   MAVGITRKLFPVETGTHQQSFNCSGCPYDCPFDCLDLPDFYFSPPPPFGDHQKQLPTIVI 60

Query: 61  VVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPIWFIN 120
           V++AL VGFF   +FY+++VK +    G  N GA ASQ DG +GE L+ NQVDHPIWFI 
Sbjct: 61  VILALFVGFFFTFSFYIIIVKWQAWRSGSGNQGAEASQLDGEDGEFLNENQVDHPIWFIT 120

Query: 121 TVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTW 180
           TVGL QSIINSI  CK+K+GEGLIEGT CSVCL+EF++ EMLRLLPKCSHAFHIGCVDTW
Sbjct: 121 TVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIGCVDTW 180

Query: 181 LRSHTTCPLCRALILTDLGA----RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGEN 240
           LR+HTTCPLCRA ILTD       RPPN G VNQ EAN    EETQMEN E      GE 
Sbjct: 181 LRTHTTCPLCRAHILTDFATSNFPRPPNSGHVNQNEANLAVIEETQMENLE------GEE 240

Query: 241 QAVVAVSGGSLENRGEVVDEQREV-VVQVGEIVPKEGITESDQNHGVEGNEILMN 279
           Q+ V+  GG +ENRG+ VDEQR+V V Q+GEIVPKEG+ ESD +  +E NE +MN
Sbjct: 241 QSAVS-GGGGVENRGDAVDEQRDVGVPQIGEIVPKEGVFESDDSK-MEVNETMMN 287

[Close]

BLAST of MC08g0217 vs. ExPASy TrEMBL
Match: A0A6J1BRC8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111005011 PE=3 SV=1)

HSP 1 Score: 576 bits (1484), Expect = 1.51e-206
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0


Query: 1   MALTISRKLFPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSV 60
           MALTISRKLFPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSV
Sbjct: 52  MALTISRKLFPSTTNCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIVVVIALSV 111

Query: 61  GFFAALNFYMVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINS 120
           GFFAALNFYMVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINS
Sbjct: 112 GFFAALNFYMVVVKCRGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQSIINS 171

Query: 121 IAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCR 180
           IAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCR
Sbjct: 172 IAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCR 231

Query: 181 ALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRG 240
           ALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRG
Sbjct: 232 ALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGEVVGENQAVVAVSGGSLENRG 291

Query: 241 EVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSS 286
           EVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSS
Sbjct: 292 EVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMNNQMDSSS 337

[Close]

BLAST of MC08g0217 vs. ExPASy TrEMBL
Match: A0A5A7VDK2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004620 PE=3 SV=1)

HSP 1 Score: 353 bits (905), Expect = 4.61e-119
Identity = 197/309 (63.75%), Postives = 223/309 (72.17%), Query Frame = 0


Query: 1   MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 60
           MA+T SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLFDHQKQL-- 60

Query: 61  PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 120
           P +++V+ L V FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 180
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRSHTTCPLCRALILTDL------GARPPNLGSVNQIEANSGGNEETQMENGEING 240
           VDTWLR+HTTCPLCRA ILTD         RPPNLG +NQ E N G NE+TQMEN  ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 241 EVVGENQA-VVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILM 286
           E V EN+  VV++S  S ENRG+VVDEQREV     EIV KEG  ESD +  VEGNE   
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQREVE----EIVQKEGNFESDDSK-VEGNESTR 300

[Close]

BLAST of MC08g0217 vs. ExPASy TrEMBL
Match: A0A1S3BAW8 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103488086 PE=3 SV=1)

HSP 1 Score: 353 bits (905), Expect = 4.61e-119
Identity = 197/309 (63.75%), Postives = 223/309 (72.17%), Query Frame = 0


Query: 1   MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 60
           MA+T SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLFDHQKQL-- 60

Query: 61  PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 120
           P +++V+ L V FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 180
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRSHTTCPLCRALILTDL------GARPPNLGSVNQIEANSGGNEETQMENGEING 240
           VDTWLR+HTTCPLCRA ILTD         RPPNLG +NQ E N G NE+TQMEN  ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 241 EVVGENQA-VVAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILM 286
           E V EN+  VV++S  S ENRG+VVDEQREV     EIV KEG  ESD +  VEGNE   
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQREVE----EIVQKEGNFESDDSK-VEGNESTR 300

[Close]

BLAST of MC08g0217 vs. ExPASy TrEMBL
Match: A0A0A0LPJ4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G369190 PE=3 SV=1)

HSP 1 Score: 349 bits (896), Expect = 1.00e-117
Identity = 191/307 (62.21%), Postives = 221/307 (71.99%), Query Frame = 0


Query: 1   MALTISRKLFP-------STTNCSGCPYDCPFDCYTFPDFDYSPPPPPP----HQNQALQ 60
           MA+T+SRKLFP        TTNCS CPYDCP+DCY FPD  +SPPPPPP    HQ Q   
Sbjct: 1   MAVTLSRKLFPVATGTALPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLSDHQKQL-- 60

Query: 61  PFIVVVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPI 120
           P  ++++ L + FF A  FY++VVKCR    G  N GA A QSDGGEGE ++ NQVDHPI
Sbjct: 61  PTTIIILILFIAFFVAFTFYIIVVKCRSWYSGSANEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 180
           WFI T GL QS+INSI  CK+K+ EGLIEGT+CSVCLSEF++ EMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRSHTTCPLCRALILTDL----GARPPNLGSVNQIEANSGGNEETQMENGEINGEV 240
           VDTWLR+HTTCPLCRA ILTD       RPPN+G +NQ E N G NE+TQMEN   N E 
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTPNSVRPPNIGPLNQNEGNLGLNEDTQMENENTNREA 240

Query: 241 VGENQAV-VAVSGGSLENRGEVVDEQREVVVQVGEIVPKEGITESDQNHGVEGNEILMN- 286
           V EN+   V++S  S ENRG+ VDEQREV     EIV KEG  ESD +  VEGNE + N 
Sbjct: 241 VRENEGGGVSISSESSENRGDAVDEQREVE----EIVQKEGNFESDDSK-VEGNESMRND 300

[Close]

BLAST of MC08g0217 vs. ExPASy TrEMBL
Match: A0A6J1K4G1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492196 PE=3 SV=1)

HSP 1 Score: 335 bits (859), Expect = 2.50e-112
Identity = 182/295 (61.69%), Postives = 213/295 (72.20%), Query Frame = 0


Query: 1   MALTISRKLFPSTT-------NCSGCPYDCPFDCYTFPDFDYSPPPPPPHQNQALQPFIV 60
           MA+ I+RKLFP  T       NCSGCPYDCPFDC   PDF +SPPPP     + L   ++
Sbjct: 1   MAVGITRKLFPVETGTHQQSFNCSGCPYDCPFDCLDLPDFYFSPPPPFGDHQKQLPTIVI 60

Query: 61  VVIALSVGFFAALNFYMVVVKCR----GPRNSGAAASQSDGGEGELLDRNQVDHPIWFIN 120
           V++AL VGFF   +FY+++VK +    G  N GA ASQ DG +GE L+ NQVDHPIWFI 
Sbjct: 61  VILALFVGFFFTFSFYIIIVKWQAWRSGSGNQGAEASQLDGEDGEFLNENQVDHPIWFIT 120

Query: 121 TVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTW 180
           TVGL QSIINSI  CK+K+GEGLIEGT CSVCL+EF++ EMLRLLPKCSHAFHIGCVDTW
Sbjct: 121 TVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIGCVDTW 180

Query: 181 LRSHTTCPLCRALILTDLGA----RPPNLGSVNQIEANSGGNEETQMENGEINGEVVGEN 240
           LR+HTTCPLCRA ILTD       RPPN G VNQ EAN    EETQMEN E      GE 
Sbjct: 181 LRTHTTCPLCRAHILTDFATSNFPRPPNSGHVNQNEANLAVIEETQMENLE------GEE 240

Query: 241 QAVVAVSGGSLENRGEVVDEQREV-VVQVGEIVPKEGITESDQNHGVEGNEILMN 279
           Q+ V+  GG +ENRG+ VDEQR+V V Q+GEIVPKEG+ ESD +  +E NE +MN
Sbjct: 241 QSAVS-GGGGVENRGDAVDEQRDVGVPQIGEIVPKEGVFESDDSK-MEVNETMMN 287

[Close]

BLAST of MC08g0217 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 164.1 bits (414), Expect = 1.7e-40
Identity = 107/263 (40.68%), Postives = 129/263 (49.05%), Query Frame = 0


Query: 1   MALTISRKLFPS-------TTNCSG--CPYDCPFDCYTFPD-FDYSPPPPP----PHQNQ 60
           MA    RKLFP+       T +CS   C   CP++CY  PD +  SP  PP    P  + 
Sbjct: 1   MARKKHRKLFPTLASETNKTLDCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSP 60

Query: 61  ALQPFI------------------------VVVIALSVGFFAALNFYMVVVKCRGPRNSG 120
              P I                         V+  L  GFF    F+   V      N G
Sbjct: 61  CPSPSISAVYLPSQDSSSSLDAISIITITGAVLAILLTGFFLVAKFFSDSV---NRVNQG 120

Query: 121 AAASQSDGG----EGELLDRNQVDHPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTEC 180
              S ++      E E  DR QVDHPIW I T GL QSIINSI  C +K+G+GLIE T+C
Sbjct: 121 TYQSDNEDNDTVMEEEFQDREQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDC 180

Query: 181 SVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPNLGSVN 222
            VCL+EF + E LRLLPKC+HAFHI C+DTWL SHT CPLCRA I       P   G V 
Sbjct: 181 PVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVE 240

[Close]

BLAST of MC08g0217 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 150.2 bits (378), Expect = 2.5e-36
Identity = 80/162 (49.38%), Postives = 103/162 (63.58%), Query Frame = 0


Query: 37  PPPPPPHQNQALQPFIVVVIALSVGFFAALNFYMVVVKCRGPRNSGAAASQSDGG----E 96
           PPPPPP +   L P  + V+  S+  F  L F++ +   +  R S A+ +  D      E
Sbjct: 30  PPPPPPRELSLLLPTSICVVG-SIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQEDE 89

Query: 97  GELLDRNQVD-HPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEML 156
            E  +R+  D H +W I TVGLH+S INSI    FK+GEG+I+GTECSVCL+EF + E L
Sbjct: 90  DETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEFEEDESL 149

Query: 157 RLLPKCSHAFHIGCVDTWLRSHTTCPLCRALILTDLGARPPN 194
           RLLPKCSHAFH+ C+DTWL SH  CPLCRA +L  L   PP+
Sbjct: 150 RLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVL--LITEPPH 188

[Close]

BLAST of MC08g0217 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-31
Identity = 63/124 (50.81%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 101 VDHPIWFINTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHA 160
           VDHPIW I T+GL+ ++I+SI  C++ + +G++EGT+CSVCLSEF + E LRLLPKC HA
Sbjct: 180 VDHPIWHIRTIGLNPTVISSIKVCQYSKKDGVVEGTDCSVCLSEFEEEETLRLLPKCKHA 239

Query: 161 FHIGCVDTWLRSHTTCPLCRALILTDLGARPPNLGSVNQIEANSGGNEETQMENGEINGE 220
           FH+ C+DTWLRSHT CPLCRA          P + +   I+ +S G EE  +   E NG+
Sbjct: 240 FHLYCIDTWLRSHTNCPLCRA----------PIVEANTMIDDHSEGLEEISVMIPEENGD 293

Query: 221 VVGE 225
              E
Sbjct: 300 DTDE 293

[Close]

BLAST of MC08g0217 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-28
Identity = 76/228 (33.33%), Postives = 118/228 (51.75%), Query Frame = 0


Query: 10  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPPP--------PHQNQALQPFIVVVIALS 69
           + S  +CS   C   CP  CY        PPPPP           + +  P ++ +I + 
Sbjct: 11  YDSYNDCSQGICNIYCPQWCYLI-----FPPPPPSFFLDDDSSSSSSSFSPLLIALIGIL 70

Query: 70  VGFFAALNFYMVVVK-C--RGPRNSGAAASQSDGGEGELLDRNQVDHPIWFINTVGLHQS 129
                 +++Y ++ K C      +S    + +  GEG      ++       N  GL++S
Sbjct: 71  TSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRIS-----TNGDGLNES 130

Query: 130 IINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGCVDTWLRSHTTC 189
           +I SI   K+K G+G ++G++CSVCLSEF + E LRLLPKC+HAFH+ C+DTWL+SH+ C
Sbjct: 131 MIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNC 190

Query: 190 PLCRALILTDLGARPPNLG-SVNQIEANSGGNEETQMENGEINGEVVG 224
           PLCRA + T +     ++G +V+ + AN   +        EIN  + G
Sbjct: 191 PLCRAFV-TGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAG 227

[Close]

BLAST of MC08g0217 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 113.6 bits (283), Expect = 2.6e-25
Identity = 77/247 (31.17%), Postives = 119/247 (48.18%), Query Frame = 0


Query: 10  FPSTTNCSG--CPYDCPFDCYTFPDFDYSPPPP-------PPHQNQALQPFIVVVIALSV 69
           + S  +CS   C   CP  CY        PPPP           +    P ++ +I +  
Sbjct: 14  YDSYRDCSQGVCSVYCPQWCYVI-----FPPPPSFYLDDEDDSSSSDFSPLLIALIGILA 73

Query: 70  GFFAALNFYMVVVK-CRGPRNSGAAASQS-----------DGGEGELLDRNQVDHPIWFI 129
             F  +++Y ++ K C   R++ ++ S +            G      + N   +P   I
Sbjct: 74  SAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNN--NNNGATNPNQTI 133

Query: 130 ---NTVGLHQSIINSIAACKFKQGEGLIEGTECSVCLSEFRQGEMLRLLPKCSHAFHIGC 189
                 GL +S+I SI   K+++ +G +E ++CSVCLSEF++ E LRLLPKC+HAFH+ C
Sbjct: 134 GGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPC 193

Query: 190 VDTWLRSHTTCPLCRALILTDLGARPPNLGSVNQIEAN---SGGNEET-----QMENGEI 225
           +DTWL+SH+ CPLCRA I+T       +L +   +  N   S G++        +EN   
Sbjct: 194 IDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNLDLENSRS 253

The following BLAST results are available for this feature:

BLAST of MC08g0217 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFY8	2.4e-39	40.68	RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2	[more]
Q9LX93	3.5e-35	49.38	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
Q7XLY8	2.4e-28	32.95	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9LF64	1.6e-27	33.33	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q9SRQ8	3.7e-24	31.17	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]

BLAST of MC08g0217 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132045.1	3.13e-206	100.00	RING-H2 finger protein ATL54-like [Momordica charantia]	[more]
XP_038884462.1	2.12e-125	65.90	RING-H2 finger protein ATL54-like [Benincasa hispida]	[more]
XP_008444871.1	9.53e-119	63.75	PREDICTED: RING-H2 finger protein ATL54-like [Cucumis melo] >KAA0065127.1 RING-H...	[more]
XP_011649673.1	2.07e-117	62.21	RING-H2 finger protein ATL54 [Cucumis sativus] >KGN62717.1 hypothetical protein ...	[more]
XP_022997232.1	5.16e-112	61.69	RING-H2 finger protein ATL54-like [Cucurbita maxima]	[more]

BLAST of MC08g0217 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BRC8	1.51e-206	100.00	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11100501...	[more]
A0A5A7VDK2	4.61e-119	63.75	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3BAW8	4.61e-119	63.75	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103488086 PE=3 ...	[more]
A0A0A0LPJ4	1.00e-117	62.21	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G369190 PE...	[more]
A0A6J1K4G1	2.50e-112	61.69	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492196 P...	[more]

BLAST of MC08g0217 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72220.1	1.7e-40	40.68	RING/U-box superfamily protein	[more]
AT5G10380.1	2.5e-36	49.38	RING/U-box superfamily protein	[more]
AT4G33565.1	1.4e-31	50.81	RING/U-box superfamily protein	[more]
AT5G17600.1	1.1e-28	33.33	RING/U-box superfamily protein	[more]
AT3G03550.1	2.6e-25	31.17	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 138..179 e-value: 1.2E-6 score: 38.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 137..180 e-value: 2.9E-13 score: 49.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 138..180 score: 12.607302
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 113..185 e-value: 6.8E-21 score: 75.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 267..286
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 264..286
None	No IPR available	PANTHER	PTHR45676:SF121	TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED	coord: 12..264
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 137..180 e-value: 1.44583E-18 score: 75.4161
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 132..199
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 12..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0217.1	MC08g0217.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane