Homology
BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match:
Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)
HSP 1 Score: 666.8 bits (1719), Expect = 1.9e-190
Identity = 327/474 (68.99%), Postives = 390/474 (82.28%), Query Frame = 0
Query: 8 HLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPRKN 67
+L + R YSR++PPP + VIRV+NN+A LG PK+GP PRQL+SLPPFPG+PLP KN
Sbjct: 12 YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71
Query: 68 -----HIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVEC--PQSGGSNGKGN 127
G SGG HVTAI+WVK+YF+EI+D IQ+HF KGL+Q+E + +
Sbjct: 72 AGADGDDGDSGG--HVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDG 131
Query: 128 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 187
PMRKIK NE+M+ G ++ +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++K
Sbjct: 132 AIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFK- 191
Query: 188 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 247
SAIIVLNKPPKLPVKGN+P+HNSMDALAAAALS+ DEGP+LVHRLDRE+SG+L+MGRT
Sbjct: 192 DSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRT 251
Query: 248 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 307
KES+ +L +FS+ + SSCKAW++AC A YQ+YWALVIG P+E+EGLISAPLSKVLLD
Sbjct: 252 KESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLD 311
Query: 308 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 367
DGKT+RVVLA + EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGT
Sbjct: 312 DGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGT 371
Query: 368 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 427
PIVGDYKYGWFVH+ WKQMP+VD+E +GKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 372 PIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 431
Query: 428 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
+VLPNIAKF+ V+ + + H DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 MVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478
BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match:
Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)
HSP 1 Score: 593.6 bits (1529), Expect = 2.0e-168
Identity = 294/477 (61.64%), Postives = 367/477 (76.94%), Query Frame = 0
Query: 6 RRHLLLAARYYSRISPPPPAYAKP-VIRV-SNNIAQLGSPKEGPKPRQLLSLPPFP---- 65
RR + A R+ SR++PP PA A P V+RV +N+A+LG PK GP+PRQLLSLPPFP
Sbjct: 5 RRRVGAAVRWLSRLAPPAPAEADPVVVRVDGSNVARLGKPKPGPRPRQLLSLPPFPGGGD 64
Query: 66 GYPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGS--NG 125
G PLP + + VTA++WVKHY ++ V+Q+HF K L+ EC S
Sbjct: 65 GDPLPGRK----AAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRLVYSECSDHEVSVETI 124
Query: 126 KGNLKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVK 185
K ++KIK N++ME G R+H+PVSVAE +I +R++TIP+ TL+PN DEI+YL+RLV
Sbjct: 125 KSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATLHPNKDEIEYLRRLVI 184
Query: 186 YKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLM 245
+K SAI+VLNKPPK+P+KGNLPVHNSMD LAAAALSY +EGPKLVHRLDRESSG+LL
Sbjct: 185 HK-DSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLF 244
Query: 246 GRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKV 305
GRTKES L WLF+++ AK++ + W+ AC A Q+YWALVIG P+EREG+ISAPLSKV
Sbjct: 245 GRTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKV 304
Query: 306 LLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEA 365
LLDDGK ERV+LA + ++ +QEA+T YRV+GPTI+GCSWIEL P T RKHQLR+HCAEA
Sbjct: 305 LLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVHCAEA 364
Query: 366 LGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLH 425
LGTPIVGDYKYGWFVH+ WKQ P+ D E +G+PYKLRRPEGL++QKGSVLSKVPLLHLH
Sbjct: 365 LGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEIQKGSVLSKVPLLHLH 424
Query: 426 CRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
CRE+V+PNIAKF+ + + P S +LLRF+A MP+HM+ISWN+MSSYLV
Sbjct: 425 CREMVIPNIAKFLS-SNGEWHENGAPWSKEKPNLLRFIAPMPAHMKISWNIMSSYLV 475
BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match:
Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)
HSP 1 Score: 264.2 bits (674), Expect = 2.8e-69
Identity = 158/402 (39.30%), Postives = 235/402 (58.46%), Query Frame = 0
Query: 72 SGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQS--GGSNGKGNLKPMRKIKS 131
S ++ TA+ W+ ++ +++Q FR ++ E S G + LK ++ +
Sbjct: 84 STDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQRSQLK---RVAA 143
Query: 132 NEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKARSAIIVLNK 191
E + G R+++P+SV + + + + +E K++ LV YK AIIVLNK
Sbjct: 144 KESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQCSDEERKFVCSLVLYK-DPAIIVLNK 203
Query: 192 PPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQW 251
P L V+G + S+D LAA+ L +D E P+LVHRLDR+ SG+L++ RT+ + L
Sbjct: 204 PHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHS 263
Query: 252 LFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVL 311
+F + +S + + +KY ALVIGCP + G ISAPL KV++DDGK+ER+ +
Sbjct: 264 IFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITV 323
Query: 312 ASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYG 371
L ++Q AITEYRV+ + +G +W+EL P T RKHQLR+HCAE LGTPIVGDYKYG
Sbjct: 324 NDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYG 383
Query: 372 WFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKF 431
W H+ + P V E K + P GLD+ G V SK P LHLH +++ LPNI++
Sbjct: 384 WQAHKA--REPFVSSENNPTK--QSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQ- 443
Query: 432 IDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSS 472
L+ K +S S+ D L+F A +PSHM++S+NL+ S
Sbjct: 444 ---LLEKMQVSSDS-DISDLDSLKFDAPLPSHMQLSFNLLKS 469
BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match:
Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)
HSP 1 Score: 176.0 bits (445), Expect = 9.9e-43
Identity = 100/278 (35.97%), Postives = 162/278 (58.27%), Query Frame = 0
Query: 74 GSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQ--VECPQSGGSNGKGNLKPMRKIKSNE 133
G TAI WV+ ++ S++Q FR ++ V + ++ +R++ + +
Sbjct: 80 GEQGATAIKWVRRCCPDLPTSLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKD 139
Query: 134 IMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKARSAIIVLNKPP 193
+ G + +PV++ E ++ + + N +EI +L+ L YK AIIV+NKPP
Sbjct: 140 QLMPGDILFLPVNLKESSVAEKTKK------FDNRNEINFLRGLEIYK-DEAIIVVNKPP 199
Query: 194 KLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQWLF 253
+PV+G + + NS+D L A+ + E P+LVHRLDR+ SGIL++GR + S + L +F
Sbjct: 200 GMPVQGGVGIKNSIDVL-ASMFEENSSEAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIF 259
Query: 254 SNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLAS 313
K++ D + +KY ALVIG PR +GL+SAPL+K+LL DGK+ER+ + +
Sbjct: 260 ----REKTADALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRA 319
Query: 314 KTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQ 350
++ + Q+A+TEYRV+ G +W+EL PRT RKHQ
Sbjct: 320 SSNAASVQDALTEYRVIESCPQGYTWLELFPRTGRKHQ 345
BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match:
Q28C59 (Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN=rpusd4 PE=2 SV=1)
HSP 1 Score: 120.2 bits (300), Expect = 6.5e-26
Identity = 90/284 (31.69%), Postives = 130/284 (45.77%), Query Frame = 0
Query: 165 NADEIKYLQRLVKYKARS--AIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYD---E 224
NA I YL+ L R ++++NKP LPV G V S+ +L A + + E
Sbjct: 37 NAPTIVYLRELKSQVVREDPELVLVNKPHGLPVHGGPTVERSVASLLPALAKHHFGWKAE 96
Query: 225 GPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALV 284
KL HRLDR+++G L++ RT E+ +Q ++ YWAL
Sbjct: 97 PLKLCHRLDRDTTGALILARTTEAAERVQQALRE---------------REVHRVYWALC 156
Query: 285 IGCPREREGLISAPLSKV------------------LLDDGKTERVVLASKTSLEASQEA 344
+G P REG++ P+ + + ++G ERV + ++ EA
Sbjct: 157 LGTPSPREGILDIPIMEKETSGPQKHYKMALSPRFRVSEEGAVERVRVP-----RSAHEA 216
Query: 345 ITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPR 404
+T YR LG +G S +EL P T KHQLR+H A L PI+GD+KY + R Q P
Sbjct: 217 VTRYRTLG-AASGASLVELHPITGVKHQLRVHLALGLNCPILGDHKYSHW-GRLAPQKP- 276
Query: 405 VDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN 426
P + R GL V + LS LHLH +L LP+
Sbjct: 277 ---------PDSVLRALGLTVPQARTLS----LHLHAVQLTLPS 284
BLAST of MC08g0159 vs. NCBI nr
Match:
XP_022131827.1 (RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia])
HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN
Sbjct: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYK
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT
Sbjct: 181 -SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 473
BLAST of MC08g0159 vs. NCBI nr
Match:
XP_022131824.1 (RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP_022131825.1 RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP_022131832.1 RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia])
HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 456/484 (94.21%), Postives = 459/484 (94.83%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGL+ GG + G
Sbjct: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVN-----PGGMSTIGW 120
Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
+K RK IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
Query: 181 YLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
YLQRLVKYK SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKD-SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 474
VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 478
BLAST of MC08g0159 vs. NCBI nr
Match:
XP_022997125.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima])
HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 437/474 (92.19%), Postives = 460/474 (97.05%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
M+QSIRRHLLLAARYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1 MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKN IG SG STHVTAI WVKHYFDEIHDSVIQSHFRKGL+QV+CPQSGG +G+GN
Sbjct: 61 YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120
Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180
Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 -SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240
Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
DGKTERVVLA+KTS EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420
Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
LVLPN+AKF++VL +KRSIS H VS+ NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473
BLAST of MC08g0159 vs. NCBI nr
Match:
XP_022131826.1 (RNA pseudouridine synthase 3, mitochondrial isoform X2 [Momordica charantia])
HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 454/484 (93.80%), Postives = 456/484 (94.21%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK GG + G
Sbjct: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK--------DPGGMSTIGW 120
Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
+K RK IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
Query: 181 YLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
YLQRLVKYK SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKD-SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 474
VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475
BLAST of MC08g0159 vs. NCBI nr
Match:
XP_022961737.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata])
HSP 1 Score: 893 bits (2308), Expect = 0.0
Identity = 436/474 (91.98%), Postives = 458/474 (96.62%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
M+QSIRRHLLLA RYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1 MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKN IG SG STHVTAI WVKHYFDEI DSVIQSHFRKGL+QV+CPQSGG +G+GN
Sbjct: 61 YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120
Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180
Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 -SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240
Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
DGKTERVVLA+KTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420
Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
LVLPN+AKF++VL +KRSIS H VS NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSYFNSDLLRFVARMPSHMRISWNLMSSYLV 473
BLAST of MC08g0159 vs. ExPASy TrEMBL
Match:
A0A6J1BS40 (RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)
HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN
Sbjct: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYK
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT
Sbjct: 181 -SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 473
BLAST of MC08g0159 vs. ExPASy TrEMBL
Match:
A0A6J1BQS0 (RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)
HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 456/484 (94.21%), Postives = 459/484 (94.83%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGL+ GG + G
Sbjct: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVN-----PGGMSTIGW 120
Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
+K RK IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
Query: 181 YLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
YLQRLVKYK SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKD-SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 474
VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 478
BLAST of MC08g0159 vs. ExPASy TrEMBL
Match:
A0A6J1K8P8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111492130 PE=4 SV=1)
HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 437/474 (92.19%), Postives = 460/474 (97.05%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
M+QSIRRHLLLAARYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1 MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKN IG SG STHVTAI WVKHYFDEIHDSVIQSHFRKGL+QV+CPQSGG +G+GN
Sbjct: 61 YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120
Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180
Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 -SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240
Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
DGKTERVVLA+KTS EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420
Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
LVLPN+AKF++VL +KRSIS H VS+ NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473
BLAST of MC08g0159 vs. ExPASy TrEMBL
Match:
A0A6J1BQJ8 (RNA pseudouridine synthase 3, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)
HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 454/484 (93.80%), Postives = 456/484 (94.21%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK GG + G
Sbjct: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK--------DPGGMSTIGW 120
Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
+K RK IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
Query: 181 YLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
YLQRLVKYK SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKD-SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 474
VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475
BLAST of MC08g0159 vs. ExPASy TrEMBL
Match:
A0A6J1HAX8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111462419 PE=4 SV=1)
HSP 1 Score: 893 bits (2308), Expect = 0.0
Identity = 436/474 (91.98%), Postives = 458/474 (96.62%), Query Frame = 0
Query: 1 MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
M+QSIRRHLLLA RYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1 MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60
Query: 61 YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
YPLPRKN IG SG STHVTAI WVKHYFDEI DSVIQSHFRKGL+QV+CPQSGG +G+GN
Sbjct: 61 YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120
Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180
Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 -SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240
Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
DGKTERVVLA+KTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420
Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
LVLPN+AKF++VL +KRSIS H VS NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSYFNSDLLRFVARMPSHMRISWNLMSSYLV 473
BLAST of MC08g0159 vs. TAIR 10
Match:
AT1G78910.1 (Pseudouridine synthase family protein )
HSP 1 Score: 666.8 bits (1719), Expect = 1.3e-191
Identity = 327/474 (68.99%), Postives = 390/474 (82.28%), Query Frame = 0
Query: 8 HLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPRKN 67
+L + R YSR++PPP + VIRV+NN+A LG PK+GP PRQL+SLPPFPG+PLP KN
Sbjct: 12 YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71
Query: 68 -----HIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVEC--PQSGGSNGKGN 127
G SGG HVTAI+WVK+YF+EI+D IQ+HF KGL+Q+E + +
Sbjct: 72 AGADGDDGDSGG--HVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDG 131
Query: 128 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 187
PMRKIK NE+M+ G ++ +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++K
Sbjct: 132 AIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFK- 191
Query: 188 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 247
SAIIVLNKPPKLPVKGN+P+HNSMDALAAAALS+ DEGP+LVHRLDRE+SG+L+MGRT
Sbjct: 192 DSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRT 251
Query: 248 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 307
KES+ +L +FS+ + SSCKAW++AC A YQ+YWALVIG P+E+EGLISAPLSKVLLD
Sbjct: 252 KESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLD 311
Query: 308 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 367
DGKT+RVVLA + EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGT
Sbjct: 312 DGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGT 371
Query: 368 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 427
PIVGDYKYGWFVH+ WKQMP+VD+E +GKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 372 PIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 431
Query: 428 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
+VLPNIAKF+ V+ + + H DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 MVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478
BLAST of MC08g0159 vs. TAIR 10
Match:
AT3G19440.1 (Pseudouridine synthase family protein )
HSP 1 Score: 264.2 bits (674), Expect = 2.0e-70
Identity = 158/402 (39.30%), Postives = 235/402 (58.46%), Query Frame = 0
Query: 72 SGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQS--GGSNGKGNLKPMRKIKS 131
S ++ TA+ W+ ++ +++Q FR ++ E S G + LK ++ +
Sbjct: 84 STDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQRSQLK---RVAA 143
Query: 132 NEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKARSAIIVLNK 191
E + G R+++P+SV + + + + +E K++ LV YK AIIVLNK
Sbjct: 144 KESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQCSDEERKFVCSLVLYK-DPAIIVLNK 203
Query: 192 PPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQW 251
P L V+G + S+D LAA+ L +D E P+LVHRLDR+ SG+L++ RT+ + L
Sbjct: 204 PHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHS 263
Query: 252 LFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVL 311
+F + +S + + +KY ALVIGCP + G ISAPL KV++DDGK+ER+ +
Sbjct: 264 IFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITV 323
Query: 312 ASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYG 371
L ++Q AITEYRV+ + +G +W+EL P T RKHQLR+HCAE LGTPIVGDYKYG
Sbjct: 324 NDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYG 383
Query: 372 WFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKF 431
W H+ + P V E K + P GLD+ G V SK P LHLH +++ LPNI++
Sbjct: 384 WQAHKA--REPFVSSENNPTK--QSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQ- 443
Query: 432 IDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSS 472
L+ K +S S+ D L+F A +PSHM++S+NL+ S
Sbjct: 444 ---LLEKMQVSSDS-DISDLDSLKFDAPLPSHMQLSFNLLKS 469
BLAST of MC08g0159 vs. TAIR 10
Match:
AT1G76050.2 (Pseudouridine synthase family protein )
HSP 1 Score: 63.5 bits (153), Expect = 5.1e-10
Identity = 77/380 (20.26%), Postives = 143/380 (37.63%), Query Frame = 0
Query: 63 LPRKNHIG------ISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSN 122
+PR N+ G + + +W+ + + + +QS R GL+ V N
Sbjct: 61 IPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTV--------N 120
Query: 123 GKGNLKPMRKIKSNEIMETGARVHIPVSVAEMK-ISRRFDTIPSGTLYPNADEIKYLQRL 182
G+ K +KS + + +++E++ + + IP +Y +
Sbjct: 121 GRVVDKVSHNVKSGD--------EVNCTISELQPLKAEAEDIPLDIVYED---------- 180
Query: 183 VKYKARSAIIVLNKPPKL---PVKGN--------------LP----------------VH 242
++V+NKP + P GN LP
Sbjct: 181 ------KHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETF 240
Query: 243 NSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCK 302
+ + + + SY P +VHRLD+ ++G+L++ + + S AHL F
Sbjct: 241 SDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFK---------- 300
Query: 303 AWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLASKTSLEASQEAI 362
+ + Y +L G P +G I P+ + + + ++ A
Sbjct: 301 -----LHTIERVYVSLTTGVPSPPQGRIEIPIGRD--SSNRIRMAAIPGGVRGGRARHAA 360
Query: 363 TEYRVLGPTINGCS-WIELCPRTSRKHQLRIHCAEALGTPIVGDYKYG----WFVHRTWK 398
+ Y+V+ G S +E T R HQ+R H A+ +G P++GD YG + K
Sbjct: 361 SRYKVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYGGTKSMALSLLQK 390
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q5XET6 | 1.9e-190 | 68.99 | RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... | [more] |
Q0J4D4 | 2.0e-168 | 61.64 | RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3... | [more] |
Q9LT72 | 2.8e-69 | 39.30 | RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... | [more] |
Q69K07 | 9.9e-43 | 35.97 | RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3... | [more] |
Q28C59 | 6.5e-26 | 31.69 | Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN... | [more] |
Match Name | E-value | Identity | Description | |
XP_022131827.1 | 0.0 | 99.58 | RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia] | [more] |
XP_022131824.1 | 0.0 | 94.21 | RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP... | [more] |
XP_022997125.1 | 0.0 | 92.19 | RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 R... | [more] |
XP_022131826.1 | 0.0 | 93.80 | RNA pseudouridine synthase 3, mitochondrial isoform X2 [Momordica charantia] | [more] |
XP_022961737.1 | 0.0 | 91.98 | RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BS40 | 0.0 | 99.58 | RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX... | [more] |
A0A6J1BQS0 | 0.0 | 94.21 | RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Momordica charantia OX... | [more] |
A0A6J1K8P8 | 0.0 | 92.19 | RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11... | [more] |
A0A6J1BQJ8 | 0.0 | 93.80 | RNA pseudouridine synthase 3, mitochondrial isoform X2 OS=Momordica charantia OX... | [more] |
A0A6J1HAX8 | 0.0 | 91.98 | RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC... | [more] |