MC08g0159 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0159
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRNA pseudouridine synthase 3, mitochondrial
LocationMC08: 1107679 .. 1113232 (-)
RNA-Seq ExpressionMC08g0159
SyntenyMC08g0159
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCTGTCGACCGCCAAAGAAGCGAGTGAGCTTTGAAGTTCCATCTTCTTCCATCATCAGCAAAACTTGGAGATGTTGAGCTCCAGCATCCACACGGTATTCGATTTCGCTAATTCAAACGCCCCACCTCTGAGAATACGAGATTTATAGCTTGAAGCCAATCGGATTCCCACCTCCATTCTCCATTCTATCTACTACACGCTCTCTCTCTCGCTGCTTCTACCGCGCTCTTTCATCTTGGATTCCATTTTCCACTCCCGCTCAGTCTCCTTTCGGAGGCCATAGAAGTCGGGTTCGTTCTTCTTTTAATTCCTGGATTTCTTATTTATTCTCCTTGCTTTTCCCGAATTATATATATTATTCTTTCGACGGGGCGGAAGTGTTTTGGTTGGTATTCTCTCGAACTACGAGCATCTCCAAAGATCATTTATGGGTTTTTTTTTGGTTTTTGTTATTATTATTTTTTATCATCTGATAGCACAATTTATTCCATATATTAGAATCAACTTGGCCTGGTTTACTGATAAACTTGGAATTCATAAATTCTGGTACCGATATTCCACGAGGAATTTGATTCCTGTCGTAATTATACCTGCACATATTTAGGGGTTGTTATTGATCATCTTCGTAGAGAGATAAGAGCTCCTCCATTTCCGTCCCTAACATTCACTTTCTGTCTTACCCAGGACTCATCCATCAATTGTAGGATTAAACAACTAGCTAGTTTTAATAGTCCACTTCTGTACAATTCCAATCTCTTACGCAATTGATTGCATGACCCACACTGTAAAACTGGTCACTGTTTCTTAGTAATGTATATATTAATTGACTTATCTGTCTGTTTTGTCTTTTTGTTTGTTTTTTTTCCTTTCCCGAGTACACAAATGTATGAATGTGGGATTGAACTTCTGACCTCTTGTGCTCATGTTTGCGTTTGTATTGCTTTTGCTCAAAATAAATGTGCTTTACAAATGAACGTTCTAAGCTTCATGAATGCTTTACATATCCAAAAAGAAGTAAAGCCGCGAATCAGGCTAATTAGAATATTTCATTTTGTAATTAGTAAAGCTAGCCATATTGCAACGGCATAGCTATATTTCATTTTGTAATTAGTAAAGCTAGCCATATTGCAACGGCATAGCTATATTTCATTTTGTAATTAGTAAAGCTAGCCATATTGCAACGGCATAGCTAGTTTAGGGGTTCAAAGGTGGCGTTTAATCAAAGCGATAGAATGTAAGTCTCCGAGGTTGAGAATAAGGGAGACACAATTGCGGAGTTGAAACAAACACACTTGCACACAGAGGGGAACGATCAAACAACTAATATAATCAAAAGACATATTGAGCATCTTTCTTCCCAACCAAAACCATTTATTACTTATTTGCATGAGAAACCTTTTGCGTTGGAGGCATTTTCAAAAGGTGCATGGGGGAGAGGTGCATTTCTTTATTCCGTACTTATAATTGCGGTCTGTAGTCATCAGGGGCCTGTTTTGCTTGGTAGAGTATTGGATTATGCAGCAGAGCATACGGCGGCATCTTTTACTCGCAGCCAGATACTACTCCAGAATTTCTCCTCCACCACCTGCATATGCTAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCATTGCCCCCATTTCCTGGTTACCCTTTGCCTCGGAAGAATCATATCGGTATATCTGGGGGGTCAACTCATGTCACTGCCATAAATTGGGTCAAGCACTATTTTGATGAAATTCATGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTAAGTACGAACACTTGCTCTTATTTTAGCTAATTGGTTTTGTCCTTTTGTCCTTTTCATGGTATGCTTATATCTAGTCTTGTAGGCTTCATGTAACTTCTTTCATGTCGAGAATCCCCAGTAAACTTTTTGAGTTGTGAGACAAGTTATAAGTTGTAAATTAGTAGCGGGAACTTAAACTATTCTTGTTTGTTTAAAGAAAATTGAGTTCCTTAGATAAATTTACAAGAGAAAATGTACATCGGACTTGTTTGCTTTTAGAATTTCAGTTTGTGTTTGTGTTATATCTTCGAGGAAAAACTTAAGGGCAACAACTTATCTGGATGGATATTCAAGGATATTTCACAACTTCAACATAACACATGGTTTTTGTCTCTCTTTATCTCTCAAGGCAATAATAAAAGCTCGATTGATGATTATCTAATCAATTCTGAGTTTAAATTTATCTCACCTTTTCCAGATCCAGGTGGAATGTCCACAATCGGGTGGATCAAATGGAAAAGGAAATTTGAAACCTATGAGAAAGGTATTGTTTTTTGAATTAACGGCCAATTCTCTTTTGAAGATTCAAAATGGAATTTCAATGTGGACACGATATTTGGTTTCATTTACTTATTCAAGTCATTCTTTGTTGGTCTGTTAACTTATTACCAGATGATTTTCAGATTAAGAGTAATGAGATTATGGAAACTGGGGCAAGAGTCCATATACCTGTGTCAGTTGCGGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGTACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTTAAGTACAAGGCAAGTACCAAGTTTCACTTGCATTCTTCTCTTTGTTGTTTCCTGCTACTAATGAATCTCCTAGTATTTTACCTATTGGTTCTAGAAGCAGTTGCTATATACTTGGTATGGAAAGATCCATGTAGATGTACTCATCTTCTTACGTACCTCGGTTGAGTTTGATGATTAATTAATTTCCTTCTCTCCATTGCTTCATTTTGCATGGCATGTGATTTCTGTTTTACGCAAGACGTGACTTCATATGATGGTTAATTAATAATTTTGTAAATTTCAGGATTCTGCTATTATTGTGTTAAACAAACCCCCCAAACTGCCTGTCAAGGTTTAATTCTCATTCTCTTTAATTTGCCTATTTCATTTCTGGTGGTTGCCGGTGGGTGGGGCGGTGGGGTGGGAGGTAATAGTAATTGCTCTGATATTTTGAAAAAATTATGGTATTGTCAGGGGAATCTTCCAGTTCATAATAGTATGGATGCATTGGCGGCTGCAGCATTATCTTATGATTATGATGAGGGTCCCAAGTTGGTAAGCTTGTTTATATTTAGGGAACCTATTTATGTAGATTTTTTTTTTCTCCAAGAAACAAAAACTTTTCATTGAGTTTGAGAAGTTGCGTATTTATATAGATATCATAATTGAGAAATCTTTGTATAAAGTATAAAAACAGCTGAGTAATTATGTTAATGTATATTTGATGGATACTTTTGGGAATAGGATGGGTTGATGCTGCGGCCATGCTATATATATATATATATATATATCTTTTTTTTATAATCGGGAGTAAGAGTTTTGTTCCCCTATACTCGAGGGCTTGAGACTTGGAAAACATTAAGGATTTTTGGTATTAAGCTCACCCGAAGGTTCAAACTTGAGACTTATAAGCTGGTATGACCAAGAGACCCAAGCCCTCGCCAACGGAGCCGTCTTTTAGGGATGCCGCGGCGCTACTACTTTGTTATTCATTTGTTTTTACAAATATGGAGACTCAATCTAGTGGAAGCAGATTTTGGTACTGTAGAATAAAAATTATTGCTTTCGGTTGTAGCAATTACGAGGCATTTATCTCTAGACATGGGATAAATTTGGGTGGATAAAGCTCCTCATTGGTTTTCTTCCTTTCATCCAAAAAATTATCCATCCAACTTTAGTGGAGAGGATGTCATTCAACTGAAGAAGTTAGAGAAATTATGTTTATTCCTTGAATAAGGTCGTACAAGCTAGTATATTTTATTCTTTTTCATCTGCAATGGCATTATTGGTTGCCTTGATCTATATCTAATGCTCTCCCTATCTGTTCTTATGGATGCTCATTATTAGGTTCATCGTTTGGATCGAGAGAGCAGCGGGATCCTCTTGATGGGGAGAACAAAAGAAAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCAAAATCTTCATGTAAGGTGCTTAATGCCATAATGCATTTAAACTAAAACAAAAGCAAAGAAGAAGATTAAACTGAGGTTTATCTTCAAGATGCTAATACGTGCTAGCTATGGCTAAAAAATGTTATAGAATTCCTTTACAATTATTGAAGTTTTATAATATGCTCAATGATGTTTTAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTGGTAATTGGTTGTCCAAGGGAAAGAGAAGGCCTAATTTCTGCTCCTCTGTCTAAGGTTCTCTCTCTCTCTGGGATCAATGTTTATGATTCTCACTCAATAGTTGCAGGTGGATTTATATAAAATAATCAGACAACAATATTTACTTGATAAGTTGAAAGTTAGAATACAATCATGTATCAGAATGGTTGATTGATTGACATGTGGTTACTCTGCCTTTAGAAACTTTGAAATCTATCTTCTAGAGCTTGAGAATATAGGTCAAACGAGTCTTTCAGGTATTAGCTCGAGTTGTTCTAACCATGTTATAATTGATTGTAGGTGCTTCTTGACGATGGGAAGACCGAGAGGGTCGTCTTGGCGAGTAAAACATCCTTGGAGGCTTCTCAGGAGGCAATCACTGAGTATCGCGTGCTTGGTCCCACGATAAATGGTTGTTCATGGATTGAACTGTGTCCCCGTACTAGCCGAAAGCATCAGGTACTTGAATCTCGCTCTCTCTCTCATGTGCACAAGCTCATGTATTGGTTTGTATCATCTTTATAATTGATCCTGCGAACCATCAAGGAAAGTGTCAATCTGAATTCATCTGATTCTTTGGAGGCAAATACTCCTTCCTATTCTTAAGTACTTCATTTTGTACACATCTGATACTGTCTTTCAGCTTCGGATCCATTGTGCTGAAGCTCTGGGCACACCTATTGTCGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAGATGCCACGTGTCGATGTTGAGCAGATAAGTGGGAAGCCGTACAAGTTGCGGAGGCCAGAAGGGCTGGACGTGCAAAAGGGGAGTGTTTTATCCAAGGTGCCATTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTTATCGACGTTTTGGTTAATAAGAGGTCGATCAGCCAGCACCCAGTATCTAGCTCGAACTCGGATCTCTTACGTTTTGTAGCGAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTATGAATAACTTTTGAGTTTTCGTAGGAATATTTTGCGTATTCAGCAGGAAATAATCAATGATTGTAATGGGTAAAAGGCCTGCCAGAATCACTAGTTTTTTTGGCTTTTCCAACTTTTTATTATTTATTTGTTATTTTTAATATAAAATATAAACATTCTTGAATCGGTACTGTGAAAATGTATAATTGTCAAATTTAAGTCAATCAGAGCATAGTTCAACA

mRNA sequence

TCTCTGTCGACCGCCAAAGAAGCGAGTGAGCTTTGAAGTTCCATCTTCTTCCATCATCAGCAAAACTTGGAGATGTTGAGCTCCAGCATCCACACGGTATTCGATTTCGCTAATTCAAACGCCCCACCTCTGAGAATACGAGATTTATAGCTTGAAGCCAATCGGATTCCCACCTCCATTCTCCATTCTATCTACTACACGCTCTCTCTCTCGCTGCTTCTACCGCGCTCTTTCATCTTGGATTCCATTTTCCACTCCCGCTCAGTCTCCTTTCGGAGGCCATAGAAGTCGGTCATCAGGGGCCTGTTTTGCTTGGTAGAGTATTGGATTATGCAGCAGAGCATACGGCGGCATCTTTTACTCGCAGCCAGATACTACTCCAGAATTTCTCCTCCACCACCTGCATATGCTAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCATTGCCCCCATTTCCTGGTTACCCTTTGCCTCGGAAGAATCATATCGGTATATCTGGGGGGTCAACTCATGTCACTGCCATAAATTGGGTCAAGCACTATTTTGATGAAATTCATGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTATCCAGGTGGAATGTCCACAATCGGGTGGATCAAATGGAAAAGGAAATTTGAAACCTATGAGAAAGATTAAGAGTAATGAGATTATGGAAACTGGGGCAAGAGTCCATATACCTGTGTCAGTTGCGGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGTACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTTAAGTACAAGGCAAGATCTGCTATTATTGTGTTAAACAAACCCCCCAAACTGCCTGTCAAGGGGAATCTTCCAGTTCATAATAGTATGGATGCATTGGCGGCTGCAGCATTATCTTATGATTATGATGAGGGTCCCAAGTTGGTTCATCGTTTGGATCGAGAGAGCAGCGGGATCCTCTTGATGGGGAGAACAAAAGAAAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCAAAATCTTCATGTAAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTGGTAATTGGTTGTCCAAGGGAAAGAGAAGGCCTAATTTCTGCTCCTCTGTCTAAGGTGCTTCTTGACGATGGGAAGACCGAGAGGGTCGTCTTGGCGAGTAAAACATCCTTGGAGGCTTCTCAGGAGGCAATCACTGAGTATCGCGTGCTTGGTCCCACGATAAATGGTTGTTCATGGATTGAACTGTGTCCCCGTACTAGCCGAAAGCATCAGCTTCGGATCCATTGTGCTGAAGCTCTGGGCACACCTATTGTCGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAGATGCCACGTGTCGATGTTGAGCAGATAAGTGGGAAGCCGTACAAGTTGCGGAGGCCAGAAGGGCTGGACGTGCAAAAGGGGAGTGTTTTATCCAAGGTGCCATTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTTATCGACGTTTTGGTTAATAAGAGGTCGATCAGCCAGCACCCAGTATCTAGCTCGAACTCGGATCTCTTACGTTTTGTAGCGAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTATGAATAACTTTTGAGTTTTCGTAGGAATATTTTGCGTATTCAGCAGGAAATAATCAATGATTGTAATGGGTAAAAGGCCTGCCAGAATCACTAGTTTTTTTGGCTTTTCCAACTTTTTATTATTTATTTGTTATTTTTAATATAAAATATAAACATTCTTGAATCGGTACTGTGAAAATGTATAATTGTCAAATTTAAGTCAATCAGAGCATAGTTCAACA

Coding sequence (CDS)

ATGCAGCAGAGCATACGGCGGCATCTTTTACTCGCAGCCAGATACTACTCCAGAATTTCTCCTCCACCACCTGCATATGCTAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCATTGCCCCCATTTCCTGGTTACCCTTTGCCTCGGAAGAATCATATCGGTATATCTGGGGGGTCAACTCATGTCACTGCCATAAATTGGGTCAAGCACTATTTTGATGAAATTCATGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTATCCAGGTGGAATGTCCACAATCGGGTGGATCAAATGGAAAAGGAAATTTGAAACCTATGAGAAAGATTAAGAGTAATGAGATTATGGAAACTGGGGCAAGAGTCCATATACCTGTGTCAGTTGCGGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGTACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTTAAGTACAAGGCAAGATCTGCTATTATTGTGTTAAACAAACCCCCCAAACTGCCTGTCAAGGGGAATCTTCCAGTTCATAATAGTATGGATGCATTGGCGGCTGCAGCATTATCTTATGATTATGATGAGGGTCCCAAGTTGGTTCATCGTTTGGATCGAGAGAGCAGCGGGATCCTCTTGATGGGGAGAACAAAAGAAAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCAAAATCTTCATGTAAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTGGTAATTGGTTGTCCAAGGGAAAGAGAAGGCCTAATTTCTGCTCCTCTGTCTAAGGTGCTTCTTGACGATGGGAAGACCGAGAGGGTCGTCTTGGCGAGTAAAACATCCTTGGAGGCTTCTCAGGAGGCAATCACTGAGTATCGCGTGCTTGGTCCCACGATAAATGGTTGTTCATGGATTGAACTGTGTCCCCGTACTAGCCGAAAGCATCAGCTTCGGATCCATTGTGCTGAAGCTCTGGGCACACCTATTGTCGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAGATGCCACGTGTCGATGTTGAGCAGATAAGTGGGAAGCCGTACAAGTTGCGGAGGCCAGAAGGGCTGGACGTGCAAAAGGGGAGTGTTTTATCCAAGGTGCCATTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTTATCGACGTTTTGGTTAATAAGAGGTCGATCAGCCAGCACCCAGTATCTAGCTCGAACTCGGATCTCTTACGTTTTGTAGCGAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTATGA

Protein sequence

MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGNLKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV
Homology
BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 1.9e-190
Identity = 327/474 (68.99%), Postives = 390/474 (82.28%), Query Frame = 0

Query: 8   HLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPRKN 67
           +L +  R YSR++PPP +    VIRV+NN+A LG PK+GP PRQL+SLPPFPG+PLP KN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 68  -----HIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVEC--PQSGGSNGKGN 127
                  G SGG  HVTAI+WVK+YF+EI+D  IQ+HF KGL+Q+E    +      +  
Sbjct: 72  AGADGDDGDSGG--HVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDG 131

Query: 128 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 187
             PMRKIK NE+M+ G ++ +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++K 
Sbjct: 132 AIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFK- 191

Query: 188 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 247
            SAIIVLNKPPKLPVKGN+P+HNSMDALAAAALS+  DEGP+LVHRLDRE+SG+L+MGRT
Sbjct: 192 DSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRT 251

Query: 248 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 307
           KES+ +L  +FS+ +   SSCKAW++AC A YQ+YWALVIG P+E+EGLISAPLSKVLLD
Sbjct: 252 KESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLD 311

Query: 308 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 367
           DGKT+RVVLA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGT
Sbjct: 312 DGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGT 371

Query: 368 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 427
           PIVGDYKYGWFVH+ WKQMP+VD+E  +GKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 372 PIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 431

Query: 428 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           +VLPNIAKF+ V+  + +   H       DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 MVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 2.0e-168
Identity = 294/477 (61.64%), Postives = 367/477 (76.94%), Query Frame = 0

Query: 6   RRHLLLAARYYSRISPPPPAYAKP-VIRV-SNNIAQLGSPKEGPKPRQLLSLPPFP---- 65
           RR +  A R+ SR++PP PA A P V+RV  +N+A+LG PK GP+PRQLLSLPPFP    
Sbjct: 5   RRRVGAAVRWLSRLAPPAPAEADPVVVRVDGSNVARLGKPKPGPRPRQLLSLPPFPGGGD 64

Query: 66  GYPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGS--NG 125
           G PLP +     +     VTA++WVKHY  ++   V+Q+HF K L+  EC     S    
Sbjct: 65  GDPLPGRK----AAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRLVYSECSDHEVSVETI 124

Query: 126 KGNLKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVK 185
           K     ++KIK N++ME G R+H+PVSVAE +I +R++TIP+ TL+PN DEI+YL+RLV 
Sbjct: 125 KSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATLHPNKDEIEYLRRLVI 184

Query: 186 YKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLM 245
           +K  SAI+VLNKPPK+P+KGNLPVHNSMD LAAAALSY  +EGPKLVHRLDRESSG+LL 
Sbjct: 185 HK-DSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLF 244

Query: 246 GRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKV 305
           GRTKES   L WLF+++  AK++ + W+ AC A  Q+YWALVIG P+EREG+ISAPLSKV
Sbjct: 245 GRTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKV 304

Query: 306 LLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEA 365
           LLDDGK ERV+LA  + ++ +QEA+T YRV+GPTI+GCSWIEL P T RKHQLR+HCAEA
Sbjct: 305 LLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVHCAEA 364

Query: 366 LGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLH 425
           LGTPIVGDYKYGWFVH+ WKQ P+ D E  +G+PYKLRRPEGL++QKGSVLSKVPLLHLH
Sbjct: 365 LGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEIQKGSVLSKVPLLHLH 424

Query: 426 CRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           CRE+V+PNIAKF+     +   +  P S    +LLRF+A MP+HM+ISWN+MSSYLV
Sbjct: 425 CREMVIPNIAKFLS-SNGEWHENGAPWSKEKPNLLRFIAPMPAHMKISWNIMSSYLV 475

BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 2.8e-69
Identity = 158/402 (39.30%), Postives = 235/402 (58.46%), Query Frame = 0

Query: 72  SGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQS--GGSNGKGNLKPMRKIKS 131
           S  ++  TA+ W+     ++  +++Q  FR   ++ E   S  G    +  LK   ++ +
Sbjct: 84  STDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQRSQLK---RVAA 143

Query: 132 NEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKARSAIIVLNK 191
            E +  G R+++P+SV     + +       +   + +E K++  LV YK   AIIVLNK
Sbjct: 144 KESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQCSDEERKFVCSLVLYK-DPAIIVLNK 203

Query: 192 PPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQW 251
           P  L V+G   +  S+D LAA+ L +D  E P+LVHRLDR+ SG+L++ RT+ +   L  
Sbjct: 204 PHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHS 263

Query: 252 LFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVL 311
           +F   +   +S     +   +  +KY ALVIGCP  + G ISAPL KV++DDGK+ER+ +
Sbjct: 264 IFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITV 323

Query: 312 ASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYG 371
                L ++Q AITEYRV+  + +G +W+EL P T RKHQLR+HCAE LGTPIVGDYKYG
Sbjct: 324 NDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYG 383

Query: 372 WFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKF 431
           W  H+   + P V  E    K  +   P GLD+  G V SK P LHLH +++ LPNI++ 
Sbjct: 384 WQAHKA--REPFVSSENNPTK--QSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQ- 443

Query: 432 IDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSS 472
              L+ K  +S      S+ D L+F A +PSHM++S+NL+ S
Sbjct: 444 ---LLEKMQVSSDS-DISDLDSLKFDAPLPSHMQLSFNLLKS 469

BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 9.9e-43
Identity = 100/278 (35.97%), Postives = 162/278 (58.27%), Query Frame = 0

Query: 74  GSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQ--VECPQSGGSNGKGNLKPMRKIKSNE 133
           G    TAI WV+    ++  S++Q  FR   ++  V   +   ++       +R++ + +
Sbjct: 80  GEQGATAIKWVRRCCPDLPTSLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKD 139

Query: 134 IMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKARSAIIVLNKPP 193
            +  G  + +PV++ E  ++ +         + N +EI +L+ L  YK   AIIV+NKPP
Sbjct: 140 QLMPGDILFLPVNLKESSVAEKTKK------FDNRNEINFLRGLEIYK-DEAIIVVNKPP 199

Query: 194 KLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQWLF 253
            +PV+G + + NS+D L A+    +  E P+LVHRLDR+ SGIL++GR + S + L  +F
Sbjct: 200 GMPVQGGVGIKNSIDVL-ASMFEENSSEAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIF 259

Query: 254 SNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLAS 313
                 K++    D   +   +KY ALVIG PR  +GL+SAPL+K+LL DGK+ER+ + +
Sbjct: 260 ----REKTADALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRA 319

Query: 314 KTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQ 350
            ++  + Q+A+TEYRV+     G +W+EL PRT RKHQ
Sbjct: 320 SSNAASVQDALTEYRVIESCPQGYTWLELFPRTGRKHQ 345

BLAST of MC08g0159 vs. ExPASy Swiss-Prot
Match: Q28C59 (Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 6.5e-26
Identity = 90/284 (31.69%), Postives = 130/284 (45.77%), Query Frame = 0

Query: 165 NADEIKYLQRLVKYKARS--AIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYD---E 224
           NA  I YL+ L     R    ++++NKP  LPV G   V  S+ +L  A   + +    E
Sbjct: 37  NAPTIVYLRELKSQVVREDPELVLVNKPHGLPVHGGPTVERSVASLLPALAKHHFGWKAE 96

Query: 225 GPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALV 284
             KL HRLDR+++G L++ RT E+   +Q                       ++ YWAL 
Sbjct: 97  PLKLCHRLDRDTTGALILARTTEAAERVQQALRE---------------REVHRVYWALC 156

Query: 285 IGCPREREGLISAPLSKV------------------LLDDGKTERVVLASKTSLEASQEA 344
           +G P  REG++  P+ +                   + ++G  ERV +       ++ EA
Sbjct: 157 LGTPSPREGILDIPIMEKETSGPQKHYKMALSPRFRVSEEGAVERVRVP-----RSAHEA 216

Query: 345 ITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPR 404
           +T YR LG   +G S +EL P T  KHQLR+H A  L  PI+GD+KY  +  R   Q P 
Sbjct: 217 VTRYRTLG-AASGASLVELHPITGVKHQLRVHLALGLNCPILGDHKYSHW-GRLAPQKP- 276

Query: 405 VDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN 426
                    P  + R  GL V +   LS    LHLH  +L LP+
Sbjct: 277 ---------PDSVLRALGLTVPQARTLS----LHLHAVQLTLPS 284

BLAST of MC08g0159 vs. NCBI nr
Match: XP_022131827.1 (RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia])

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
            SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT
Sbjct: 181 -SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
           LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of MC08g0159 vs. NCBI nr
Match: XP_022131824.1 (RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP_022131825.1 RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP_022131832.1 RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 456/484 (94.21%), Postives = 459/484 (94.83%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGL+       GG +  G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVN-----PGGMSTIGW 120

Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
           YLQRLVKYK  SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKD-SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240

Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
           SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300

Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
           SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360

Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
           RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420

Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 474
           VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 478

BLAST of MC08g0159 vs. NCBI nr
Match: XP_022997125.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 437/474 (92.19%), Postives = 460/474 (97.05%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLLAARYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKN IG SG STHVTAI WVKHYFDEIHDSVIQSHFRKGL+QV+CPQSGG +G+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
            SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 -SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLA+KTS EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
           LVLPN+AKF++VL +KRSIS H VS+ NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of MC08g0159 vs. NCBI nr
Match: XP_022131826.1 (RNA pseudouridine synthase 3, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 454/484 (93.80%), Postives = 456/484 (94.21%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK          GG +  G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK--------DPGGMSTIGW 120

Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
           YLQRLVKYK  SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKD-SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240

Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
           SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300

Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
           SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360

Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
           RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420

Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 474
           VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475

BLAST of MC08g0159 vs. NCBI nr
Match: XP_022961737.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 893 bits (2308), Expect = 0.0
Identity = 436/474 (91.98%), Postives = 458/474 (96.62%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLLA RYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKN IG SG STHVTAI WVKHYFDEI DSVIQSHFRKGL+QV+CPQSGG +G+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
            SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 -SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLA+KTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
           LVLPN+AKF++VL +KRSIS H VS  NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSYFNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of MC08g0159 vs. ExPASy TrEMBL
Match: A0A6J1BS40 (RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
            SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT
Sbjct: 181 -SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
           LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of MC08g0159 vs. ExPASy TrEMBL
Match: A0A6J1BQS0 (RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 456/484 (94.21%), Postives = 459/484 (94.83%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGL+       GG +  G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVN-----PGGMSTIGW 120

Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
           YLQRLVKYK  SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKD-SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240

Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
           SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300

Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
           SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360

Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
           RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420

Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 474
           VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 478

BLAST of MC08g0159 vs. ExPASy TrEMBL
Match: A0A6J1K8P8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111492130 PE=4 SV=1)

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 437/474 (92.19%), Postives = 460/474 (97.05%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLLAARYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKN IG SG STHVTAI WVKHYFDEIHDSVIQSHFRKGL+QV+CPQSGG +G+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
            SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 -SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLA+KTS EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
           LVLPN+AKF++VL +KRSIS H VS+ NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of MC08g0159 vs. ExPASy TrEMBL
Match: A0A6J1BQJ8 (RNA pseudouridine synthase 3, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 454/484 (93.80%), Postives = 456/484 (94.21%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK          GG +  G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK--------DPGGMSTIGW 120

Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKARSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
           YLQRLVKYK  SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKD-SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240

Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
           SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300

Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
           SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360

Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
           RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420

Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 474
           VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475

BLAST of MC08g0159 vs. ExPASy TrEMBL
Match: A0A6J1HAX8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111462419 PE=4 SV=1)

HSP 1 Score: 893 bits (2308), Expect = 0.0
Identity = 436/474 (91.98%), Postives = 458/474 (96.62%), Query Frame = 0

Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLLA RYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120
           YPLPRKN IG SG STHVTAI WVKHYFDEI DSVIQSHFRKGL+QV+CPQSGG +G+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
            SAI+VLNKPP+LPVKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 -SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLA+KTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 474
           LVLPN+AKF++VL +KRSIS H VS  NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSYFNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of MC08g0159 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 666.8 bits (1719), Expect = 1.3e-191
Identity = 327/474 (68.99%), Postives = 390/474 (82.28%), Query Frame = 0

Query: 8   HLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPRKN 67
           +L +  R YSR++PPP +    VIRV+NN+A LG PK+GP PRQL+SLPPFPG+PLP KN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 68  -----HIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVEC--PQSGGSNGKGN 127
                  G SGG  HVTAI+WVK+YF+EI+D  IQ+HF KGL+Q+E    +      +  
Sbjct: 72  AGADGDDGDSGG--HVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDG 131

Query: 128 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 187
             PMRKIK NE+M+ G ++ +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++K 
Sbjct: 132 AIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFK- 191

Query: 188 RSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 247
            SAIIVLNKPPKLPVKGN+P+HNSMDALAAAALS+  DEGP+LVHRLDRE+SG+L+MGRT
Sbjct: 192 DSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRT 251

Query: 248 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 307
           KES+ +L  +FS+ +   SSCKAW++AC A YQ+YWALVIG P+E+EGLISAPLSKVLLD
Sbjct: 252 KESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLD 311

Query: 308 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 367
           DGKT+RVVLA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGT
Sbjct: 312 DGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGT 371

Query: 368 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 427
           PIVGDYKYGWFVH+ WKQMP+VD+E  +GKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 372 PIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 431

Query: 428 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           +VLPNIAKF+ V+  + +   H       DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 MVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

BLAST of MC08g0159 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 264.2 bits (674), Expect = 2.0e-70
Identity = 158/402 (39.30%), Postives = 235/402 (58.46%), Query Frame = 0

Query: 72  SGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQS--GGSNGKGNLKPMRKIKS 131
           S  ++  TA+ W+     ++  +++Q  FR   ++ E   S  G    +  LK   ++ +
Sbjct: 84  STDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQRSQLK---RVAA 143

Query: 132 NEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKARSAIIVLNK 191
            E +  G R+++P+SV     + +       +   + +E K++  LV YK   AIIVLNK
Sbjct: 144 KESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQCSDEERKFVCSLVLYK-DPAIIVLNK 203

Query: 192 PPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQW 251
           P  L V+G   +  S+D LAA+ L +D  E P+LVHRLDR+ SG+L++ RT+ +   L  
Sbjct: 204 PHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHS 263

Query: 252 LFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVL 311
           +F   +   +S     +   +  +KY ALVIGCP  + G ISAPL KV++DDGK+ER+ +
Sbjct: 264 IFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITV 323

Query: 312 ASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYG 371
                L ++Q AITEYRV+  + +G +W+EL P T RKHQLR+HCAE LGTPIVGDYKYG
Sbjct: 324 NDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYG 383

Query: 372 WFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKF 431
           W  H+   + P V  E    K  +   P GLD+  G V SK P LHLH +++ LPNI++ 
Sbjct: 384 WQAHKA--REPFVSSENNPTK--QSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQ- 443

Query: 432 IDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSS 472
              L+ K  +S      S+ D L+F A +PSHM++S+NL+ S
Sbjct: 444 ---LLEKMQVSSDS-DISDLDSLKFDAPLPSHMQLSFNLLKS 469

BLAST of MC08g0159 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 63.5 bits (153), Expect = 5.1e-10
Identity = 77/380 (20.26%), Postives = 143/380 (37.63%), Query Frame = 0

Query: 63  LPRKNHIG------ISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSN 122
           +PR N+ G      +      +   +W+    + +  + +QS  R GL+ V        N
Sbjct: 61  IPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTV--------N 120

Query: 123 GKGNLKPMRKIKSNEIMETGARVHIPVSVAEMK-ISRRFDTIPSGTLYPNADEIKYLQRL 182
           G+   K    +KS +         +  +++E++ +    + IP   +Y +          
Sbjct: 121 GRVVDKVSHNVKSGD--------EVNCTISELQPLKAEAEDIPLDIVYED---------- 180

Query: 183 VKYKARSAIIVLNKPPKL---PVKGN--------------LP----------------VH 242
                   ++V+NKP  +   P  GN              LP                  
Sbjct: 181 ------KHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETF 240

Query: 243 NSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCK 302
           +  + +  +  SY     P +VHRLD+ ++G+L++ + + S AHL   F           
Sbjct: 241 SDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFK---------- 300

Query: 303 AWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLASKTSLEASQEAI 362
                 +   + Y +L  G P   +G I  P+ +      +     +        ++ A 
Sbjct: 301 -----LHTIERVYVSLTTGVPSPPQGRIEIPIGRD--SSNRIRMAAIPGGVRGGRARHAA 360

Query: 363 TEYRVLGPTINGCS-WIELCPRTSRKHQLRIHCAEALGTPIVGDYKYG----WFVHRTWK 398
           + Y+V+     G S  +E    T R HQ+R H A+ +G P++GD  YG      +    K
Sbjct: 361 SRYKVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYGGTKSMALSLLQK 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5XET61.9e-19068.99RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q0J4D42.0e-16861.64RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q9LT722.8e-6939.30RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q69K079.9e-4335.97RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q28C596.5e-2631.69Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN... [more]
Match NameE-valueIdentityDescription
XP_022131827.10.099.58RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia][more]
XP_022131824.10.094.21RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP... [more]
XP_022997125.10.092.19RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 R... [more]
XP_022131826.10.093.80RNA pseudouridine synthase 3, mitochondrial isoform X2 [Momordica charantia][more]
XP_022961737.10.091.98RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1... [more]
Match NameE-valueIdentityDescription
A0A6J1BS400.099.58RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX... [more]
A0A6J1BQS00.094.21RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Momordica charantia OX... [more]
A0A6J1K8P80.092.19RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1BQJ80.093.80RNA pseudouridine synthase 3, mitochondrial isoform X2 OS=Momordica charantia OX... [more]
A0A6J1HAX80.091.98RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G78910.11.3e-19168.99Pseudouridine synthase family protein [more]
AT3G19440.12.0e-7039.30Pseudouridine synthase family protein [more]
AT1G76050.25.1e-1020.26Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 184..355
e-value: 2.3E-22
score: 79.8
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 184..369
e-value: 3.02719E-42
score: 146.328
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 176..431
e-value: 6.7E-46
score: 158.6
NoneNo IPR availablePANTHERPTHR21600:SF53RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIALcoord: 6..471
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 6..471
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 181..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0159.1MC08g0159.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding