MC08g0093 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0093
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProteasome subunit beta
LocationMC08: 592789 .. 596572 (+)
RNA-Seq ExpressionMC08g0093
SyntenyMC08g0093
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTTTTTGAGTGGTTATTTTGTGATATTTAATTTTTGTTATATATTAAATTAGAACGAGAGAGTTGGGTTCAAATTGGGCCAACAAGCCCAGAGGCCCAATACGTTTCCACCGTCTAAGTTGAAAAATGTGGCCCAAGCCCATTAAGCCCGTTGGTTTCCTGGAGGACAAGAAACTCGCCTTCGGCTTTCCCGTAAATATCCTTAATTTCGCAGCATTTTTCCCTCCATTCCGATCATTTCCTTCGACGTTGTGGATCTTTATTTGCTTTCTGTTCGCGTTTTCTTCTCTCGTTGCTCTAGATTCTAGTTAAAGGTAAAGTTGAAGTAGCACGACTTCGCTCTTATGTTTCTTCGATTTCATATGCGGATTCATGAATCATCTCAGATTGGAATCAATGTCTATCATTAGGGTCTTGATATCGACCTGTTTTTGCAAAAAATTGCATGCATTTTGATGATTTTTAGGGTCTGCTTTGATTTATGGGAGAAATTTAGGAGAGGTACTAAACGTCTGATTATACGATTCAGTTTTTGATGGTCGAGGAGCTCCAAGTATCTTTAAATGTCAAATCTATTATGATGGCTAATTAGTGTTTCTCTTGGATGCAGTCGTTCTGATAAATTTTCCTGGCACGGTTGAATCTTATTTTTCTTCGGTGTTCTGAATTAAGTTTTCGTAATTGACTTATGTGGAGTAGAATTAGCTCAGTCGTCGGCATAGCAGGGGGTGGTTCTGGGGATTTTTCTTTTTCTAATTTTTATACTGTCATGTGGATAACTTATTTGTATCACGAGCTCTGTACTTCAGCTCGATTTTTGGTCGTAACAGGAGTTGAAACATGAAGCTTGATACCAGTGGTCTTCAAACGACTGCTCCGCTGTTTGGGCCAAAAATGGAGATTTTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAATGATGTAATTTGTAAAGCATTTATCATACGGCTTCTGGGCTGCGTACTGTTCTGTATTATTGTTTTTTCTGACTTTGATTTTCTCCGTATTTCAGTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCGGCAAAAGGAACAACCACTCTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCCGATTCCCGTGCTAGCATGGGAGGCTATATATGTATGTTTACTTAATTACATTTGTTCTTCAAACAAATTTGAAATTCATTTTCTAGATGTTTTGTGGGTCGAATACTTCAACCTTTTTAGTGATTACGAGGTTAATTTTCTTCCATTATGTGTCCATATATGATATAAATGAGGTGCTATATGCAGCATGGATGAATTTATTCACTATTTCCCGTTTAGCTTTATGCTGACTTGTCTTCCCCTCCCTCCAAGTTTTGTAAGTTATCGTGTTGTTATTTTTGCACCTTTCTTCCTTTTCAACTCAATATTCGTTTTTGAATTTTTTCAGCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAGTTTTGGCATAGAAACCTCGGTGTCAAGGTAATTGGCTGATATGCAATTACTTGTAGTTGTAAGACTATCAACATTTTAGTAAGAATGGGTTACCTTATACTACTATCATTGTGAACTCGATAGGTACGCTTGCTTAATATATGAGGTTAATCTTGATTAATTGCCTTCACTTTTAGAGTTTTCCAGTCTCAACAACTCAACTCAACATGTAAATGTGCAATCAGATATTCATCCTTCCCTAATTGGATTAATTGTGCCCTGCCAATGTTGATATTTTGGATTTTTTTAATCTTGTGATTAGCTGATCATAGTGTTTAACCTGATTTAGTGCCGCCGACATGAACTAGCAAATAAACGCCGAATTTCAGTCGCAGGAGCATCAAAGTTATTGGCTAACATTCTGTACTCCTATCGCGGAATGGGTCTCTCTGTCGGGACTATGATTGCTGGATGGGATGAAACAGTAATTACATATTTATTTATTTTCATTTTCCCACTTTATGGTGATCTATAATTTACCTAATTCTGTGCTTTGTAGGGTCCTGGTCTATATTACGTAGACAGCGAGGGGGGAAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGGTAAGTATGATTCTCAATTATCATGCTGTCTTTGAAAATTTCTCAATTCAGTAATTTTGTAATTGAACCTATCTTGCCAAGATAAATCATGTAGTCTTCCCACATGTCTGTGAAATGATGGCAAAAAACATGATCTTATGTTTTGTTTGTGCAAGGGTGACATGCTGGTTATATATATATCTACATATATATATATATACATATTATATCGTTAGAATCCCAAATACAGTAAATGGAACTAGATTACTATATTGTTATATCAAAGAGATAGTTACAATATCAATAGCCCTTCGAGAGGGCTATCTCTCTCCCAAGAACTCTCTCAAGTTTCTTCACTATAAACTATTCCCAAAGTTATCCTTCTCCTCTCCCTACTACTATTTATAACACCCCTAATTAATACCACAATATCCCCCAATAATATACTAATAATGCTCCCAAACTATCTCTACTAGGACTCTCATATTACCCCGCCCTTCAAAGACAACCATAATACCCCCATATTAATATTCTCAAACTAGTGAGGACTGTCTCTCTCACACACATATATATATTTGAAACATGAAAATTTCTCTGATTTGATATATATTGAAAGTTACACTGAGGTAGTACAAAAGATAGAAACAAAGAGAACTTTTAACTCCCCCACCAAACTAAATATACAATTAATTCCACATCCCTTCTCTACAATACCTCTCCCACAGCCTTATGTAAAAAGAAGCTCAGCAAAAAGCCTCCCAATTAGAATTAATTGAAAGCTATATCTCAACTTGTAATTGTGATTATATTTCTCGGTCTTTCCTGTTGTCGAGCTAACGTACTGCTTTGCGTGATGATGTTGGTACAGACATTTCTTCGATATTCTTTTCTCTTACCCCTCTTCTTTCCCCTCTTTTTGAGACAATTGTCCATCATTTCATCTAATGTGAGTTTATCTATGATTTTATGTATGTTAATTCATGAATACCCTATTGTGTGTGCTACTGAGCAGATATAGGTATGACTTATCTGTTGAAGAAGCTGCTGAGCTGGCTAGACGAGCCATTTATCATGCAACTTTTCGAGATGGAGCAAGTGGTGGAGTTGCCAGCGGTATCCTTCTGTCTAGATATTTATCTCTCTTTGTAATACACACACATTTATGTGCTTGTGCAAATTCCCCTCTTCTCACTCATGATTTGAATGTCACCGATCAGTTTATTATGTGGGACCAAATGGATGGAAGAAGCTCTCTGGCGATGATGTGGGAGAACTTCACTACAACTACTATCCAGTGACACCAAGTACAGTGGATCAGGAAATGGTTGAAGTAACCGGTGCTTGACAATCCCATATAAACACAAGCAGGTCGAGACATTCTCTCTCTGTTCATTTATACTAAGAGGGGAAGAATTGATTCTAAAGAGATGCTGAAGATGTGAACTCTTAGTTTATGTGTGCACTCGCAGATTCTAAAAAAAGGTTGGAGTTGATCTAGGCTTAAAAACTAGTGTTGTTTCAAGGATATTTCTGTCATGGTATTCGAGTTATCGCTGCTTTTGAATATTGTCAACCTTTCCTAAATTTTTAAATCCATTTCTTAATTGGACT

mRNA sequence

GGTTTTTGAGTGGTTATTTTGTGATATTTAATTTTTGTTATATATTAAATTAGAACGAGAGAGTTGGGTTCAAATTGGGCCAACAAGCCCAGAGGCCCAATACGTTTCCACCGTCTAAGTTGAAAAATGTGGCCCAAGCCCATTAAGCCCGTTGGTTTCCTGGAGGACAAGAAACTCGCCTTCGGCTTTCCCGTAAATATCCTTAATTTCGCAGCATTTTTCCCTCCATTCCGATCATTTCCTTCGACGTTGTGGATCTTTATTTGCTTTCTGTTCGCGTTTTCTTCTCTCGTTGCTCTAGATTCTAGTTAAAGGAGTTGAAACATGAAGCTTGATACCAGTGGTCTTCAAACGACTGCTCCGCTGTTTGGGCCAAAAATGGAGATTTTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAATGATTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCGGCAAAAGGAACAACCACTCTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCCGATTCCCGTGCTAGCATGGGAGGCTATATATCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAGTTTTGGCATAGAAACCTCGGTGTCAAGTGCCGCCGACATGAACTAGCAAATAAACGCCGAATTTCAGTCGCAGGAGCATCAAAGTTATTGGCTAACATTCTGTACTCCTATCGCGGAATGGGTCTCTCTGTCGGGACTATGATTGCTGGATGGGATGAAACAGGTCCTGGTCTATATTACGTAGACAGCGAGGGGGGAAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGATATAGGTATGACTTATCTGTTGAAGAAGCTGCTGAGCTGGCTAGACGAGCCATTTATCATGCAACTTTTCGAGATGGAGCAAGTGGTGGAGTTGCCAGCGTTTATTATGTGGGACCAAATGGATGGAAGAAGCTCTCTGGCGATGATGTGGGAGAACTTCACTACAACTACTATCCAGTGACACCAAGTACAGTGGATCAGGAAATGGTTGAAGTAACCGGTGCTTGACAATCCCATATAAACACAAGCAGGTCGAGACATTCTCTCTCTGTTCATTTATACTAAGAGGGGAAGAATTGATTCTAAAGAGATGCTGAAGATGTGAACTCTTAGTTTATGTGTGCACTCGCAGATTCTAAAAAAAGGTTGGAGTTGATCTAGGCTTAAAAACTAGTGTTGTTTCAAGGATATTTCTGTCATGGTATTCGAGTTATCGCTGCTTTTGAATATTGTCAACCTTTCCTAAATTTTTAAATCCATTTCTTAATTGGACT

Coding sequence (CDS)

ATGAAGCTTGATACCAGTGGTCTTCAAACGACTGCTCCGCTGTTTGGGCCAAAAATGGAGATTTTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAATGATTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCGGCAAAAGGAACAACCACTCTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCCGATTCCCGTGCTAGCATGGGAGGCTATATATCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAGTTTTGGCATAGAAACCTCGGTGTCAAGTGCCGCCGACATGAACTAGCAAATAAACGCCGAATTTCAGTCGCAGGAGCATCAAAGTTATTGGCTAACATTCTGTACTCCTATCGCGGAATGGGTCTCTCTGTCGGGACTATGATTGCTGGATGGGATGAAACAGGTCCTGGTCTATATTACGTAGACAGCGAGGGGGGAAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGATATAGGTATGACTTATCTGTTGAAGAAGCTGCTGAGCTGGCTAGACGAGCCATTTATCATGCAACTTTTCGAGATGGAGCAAGTGGTGGAGTTGCCAGCGTTTATTATGTGGGACCAAATGGATGGAAGAAGCTCTCTGGCGATGATGTGGGAGAACTTCACTACAACTACTATCCAGTGACACCAAGTACAGTGGATCAGGAAATGGTTGAAGTAACCGGTGCTTGA

Protein sequence

MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA
Homology
BLAST of MC08g0093 vs. ExPASy Swiss-Prot
Match: O24361 (Proteasome subunit beta type-5 OS=Spinacia oleracea OX=3562 PE=2 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 1.4e-134
Identity = 228/272 (83.82%), Postives = 252/272 (92.65%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGL++TAP+F     + +G    PSF++PN  DFDGFQKEA+QMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLESTAPIFRRSDFVFDGLQMTPSFDLPNPTDFDGFQKEAVQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KCR 
Sbjct: 61  FIFKHGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRL 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISV GASKLLANILY+YRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG R
Sbjct: 121 HELANKRRISVTGASKLLANILYNYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGMR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GY+YD++VEEA+ELARRAIYHAT+RDGASGGV SVY+VGP+GWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYKYDMTVEEASELARRAIYHATYRDGASGGVVSVYHVGPDGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 273
           K++GDDVG+LH+ YYPV P+TV+QEMVEV GA
Sbjct: 241 KVTGDDVGDLHFQYYPVVPATVEQEMVEVVGA 272

BLAST of MC08g0093 vs. ExPASy Swiss-Prot
Match: Q9LIP2 (Proteasome subunit beta type-5-B OS=Arabidopsis thaliana OX=3702 GN=PBE2 PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 4.5e-133
Identity = 229/270 (84.81%), Postives = 252/270 (93.33%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSGL+TT P+  FG   E+L+GFS+APSF++P + DFDGFQK+A++MVKPAKGTTT
Sbjct: 1   MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNG 240
            RFSVGSGSPYAYGVLDSGY++D+SVEEA+ELARR+IYHATFRDGASGGVASVY+VGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQG 240

Query: 241 WKKLSGDDVGELHYNYYPVTPSTVDQEMVE 269
           W KLSGDDVGELHY+YYPV P T +  M E
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPITAEHVMEE 270

BLAST of MC08g0093 vs. ExPASy Swiss-Prot
Match: O23717 (Proteasome subunit beta type-5-A OS=Arabidopsis thaliana OX=3702 GN=PBE1 PE=1 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 3.4e-128
Identity = 222/272 (81.62%), Postives = 247/272 (90.81%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG +T+ P+  FG   ++L+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNG 240
            RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+IYHATFRDGASGGVASVY+VGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEG 240

Query: 241 WKKLSGDDVGELHYNYYPVTPSTVDQEMVEVT 271
           W KLSGDDVGELHY+YYPV P+T +Q M E T
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPATAEQVMEEAT 272

BLAST of MC08g0093 vs. ExPASy Swiss-Prot
Match: O55234 (Proteasome subunit beta type-5 OS=Mus musculus OX=10090 GN=Psmb5 PE=1 SV=3)

HSP 1 Score: 291.2 bits (744), Expect = 1.2e-77
Identity = 146/232 (62.93%), Postives = 178/232 (76.72%), Query Frame = 0

Query: 27  AAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSV 86
           AAPS+ +P        ++  I+M+    GTTTLAF F  GV+VAADSRA+ G YI+SQ+V
Sbjct: 42  AAPSWGVP--------EEPRIEML---HGTTTLAFKFLHGVIVAADSRATAGAYIASQTV 101

Query: 87  KKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYR 146
           KK+IEINPY+LGTMAGGAADC FW R L  +CR +EL NK RISVA ASKLLAN++Y Y+
Sbjct: 102 KKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYK 161

Query: 147 GMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEE 206
           GMGLS+GTMI GWD+ GPGLYYVDSEG R+ GT FSVGSGS YAYGV+D GY YDL VEE
Sbjct: 162 GMGLSMGTMICGWDKRGPGLYYVDSEGNRISGTAFSVGSGSVYAYGVMDRGYSYDLKVEE 221

Query: 207 AAELARRAIYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYNYYPVT 259
           A +LARRAIY AT+RD  SGG  ++Y+V  +GW ++S D+V +LH  Y  V+
Sbjct: 222 AYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHDKYSSVS 262

BLAST of MC08g0093 vs. ExPASy Swiss-Prot
Match: P28074 (Proteasome subunit beta type-5 OS=Homo sapiens OX=9606 GN=PSMB5 PE=1 SV=3)

HSP 1 Score: 290.4 bits (742), Expect = 2.1e-77
Identity = 144/233 (61.80%), Postives = 177/233 (75.97%), Query Frame = 0

Query: 27  AAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSV 86
           AAP + +P        ++  I+M+    GTTTLAF F+ GV+VAADSRA+ G YI+SQ+V
Sbjct: 42  AAPGWGVP--------EEPGIEML---HGTTTLAFKFRHGVIVAADSRATAGAYIASQTV 101

Query: 87  KKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYR 146
           KK+IEINPY+LGTMAGGAADC FW R L  +CR +EL NK RISVA ASKLLAN++Y Y+
Sbjct: 102 KKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYK 161

Query: 147 GMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEE 206
           GMGLS+GTMI GWD+ GPGLYYVDSEG R+ G  FSVGSGS YAYGV+D GY YDL VE+
Sbjct: 162 GMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQ 221

Query: 207 AAELARRAIYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYNYYPVTP 260
           A +LARRAIY AT+RD  SGG  ++Y+V  +GW ++S D+V +LH  Y   TP
Sbjct: 222 AYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP 263

BLAST of MC08g0093 vs. NCBI nr
Match: XP_022131817.1 (proteasome subunit beta type-5 [Momordica charantia] >XP_022131818.1 proteasome subunit beta type-5 [Momordica charantia])

HSP 1 Score: 548 bits (1412), Expect = 9.39e-197
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA
Sbjct: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272

BLAST of MC08g0093 vs. NCBI nr
Match: XP_038883961.1 (proteasome subunit beta type-5 isoform X1 [Benincasa hispida])

HSP 1 Score: 536 bits (1380), Expect = 7.11e-192
Identity = 264/272 (97.06%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKME LEGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTAA 272

BLAST of MC08g0093 vs. NCBI nr
Match: XP_022961789.1 (proteasome subunit beta type-5-like [Cucurbita moschata] >XP_022997234.1 proteasome subunit beta type-5-like [Cucurbita maxima] >XP_022997235.1 proteasome subunit beta type-5-like [Cucurbita maxima])

HSP 1 Score: 535 bits (1379), Expect = 1.01e-191
Identity = 263/272 (96.69%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKME +EGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTAA 272

BLAST of MC08g0093 vs. NCBI nr
Match: XP_008445492.1 (PREDICTED: proteasome subunit beta type-5 [Cucumis melo] >KAA0064739.1 proteasome subunit beta type-5 [Cucumis melo var. makuwa])

HSP 1 Score: 534 bits (1375), Expect = 4.11e-191
Identity = 262/272 (96.32%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAP+FGP+ME LEGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPMFGPRMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMTEVTAA 272

BLAST of MC08g0093 vs. NCBI nr
Match: XP_023002169.1 (proteasome subunit beta type-5-B isoform X1 [Cucurbita maxima])

HSP 1 Score: 533 bits (1372), Expect = 1.05e-190
Identity = 262/268 (97.76%), Postives = 267/268 (99.63%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQT+APLFGPKMEILEGFSAAPSFE+PNS+DFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTSAPLFGPKMEILEGFSAAPSFEVPNSSDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVE 268
           KLSGDDVGELHYNYYPVTP+TVDQEM E
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAE 268

BLAST of MC08g0093 vs. ExPASy TrEMBL
Match: A0A6J1BS30 (Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111004886 PE=3 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 4.55e-197
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA
Sbjct: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272

BLAST of MC08g0093 vs. ExPASy TrEMBL
Match: A0A6J1KAW4 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111492198 PE=3 SV=1)

HSP 1 Score: 535 bits (1379), Expect = 4.89e-192
Identity = 263/272 (96.69%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKME +EGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTAA 272

BLAST of MC08g0093 vs. ExPASy TrEMBL
Match: A0A6J1HF19 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462451 PE=3 SV=1)

HSP 1 Score: 535 bits (1379), Expect = 4.89e-192
Identity = 263/272 (96.69%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKME +EGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTAA 272

BLAST of MC08g0093 vs. ExPASy TrEMBL
Match: A0A5A7VGZ1 (Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00430 PE=3 SV=1)

HSP 1 Score: 534 bits (1375), Expect = 1.99e-191
Identity = 262/272 (96.32%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAP+FGP+ME LEGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPMFGPRMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMTEVTAA 272

BLAST of MC08g0093 vs. ExPASy TrEMBL
Match: A0A1S3BCV3 (Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103488492 PE=3 SV=1)

HSP 1 Score: 534 bits (1375), Expect = 1.99e-191
Identity = 262/272 (96.32%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAP+FGP+ME LEGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPMFGPRMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA 272
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMTEVTAA 272

BLAST of MC08g0093 vs. TAIR 10
Match: AT3G26340.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 475.3 bits (1222), Expect = 3.2e-134
Identity = 229/270 (84.81%), Postives = 252/270 (93.33%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSGL+TT P+  FG   E+L+GFS+APSF++P + DFDGFQK+A++MVKPAKGTTT
Sbjct: 1   MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNG 240
            RFSVGSGSPYAYGVLDSGY++D+SVEEA+ELARR+IYHATFRDGASGGVASVY+VGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQG 240

Query: 241 WKKLSGDDVGELHYNYYPVTPSTVDQEMVE 269
           W KLSGDDVGELHY+YYPV P T +  M E
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPITAEHVMEE 270

BLAST of MC08g0093 vs. TAIR 10
Match: AT1G13060.1 (20S proteasome beta subunit E1 )

HSP 1 Score: 459.1 bits (1180), Expect = 2.4e-129
Identity = 222/272 (81.62%), Postives = 247/272 (90.81%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG +T+ P+  FG   ++L+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNG 240
            RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+IYHATFRDGASGGVASVY+VGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEG 240

Query: 241 WKKLSGDDVGELHYNYYPVTPSTVDQEMVEVT 271
           W KLSGDDVGELHY+YYPV P+T +Q M E T
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPATAEQVMEEAT 272

BLAST of MC08g0093 vs. TAIR 10
Match: AT1G13060.2 (20S proteasome beta subunit E1 )

HSP 1 Score: 445.7 bits (1145), Expect = 2.7e-125
Identity = 222/296 (75.00%), Postives = 247/296 (83.45%), Query Frame = 0

Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG +T+ P+  FG   ++L+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYIS------------------------SQSVKKIIEINP 120
           LAFIFK GVMVAADSRASMGGYIS                        SQSVKKIIEINP
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISVSSLKNSSKNMHPFDIVDFGRNTTSQSVKKIIEINP 120

Query: 121 YMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGT 180
           YMLGTMAGGAADCQFWHRNLG+KCR HELANKRRISV+GASKLLAN+LYSYRGMGLSVGT
Sbjct: 121 YMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGT 180

Query: 181 MIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRA 240
           MIAGWDETGPGLYYVD+EGGRLKG RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+
Sbjct: 181 MIAGWDETGPGLYYVDNEGGRLKGDRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRS 240

Query: 241 IYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMVEVT 271
           IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHY+YYPV P+T +Q M E T
Sbjct: 241 IYHATFRDGASGGVASVYHVGPEGWTKLSGDDVGELHYHYYPVAPATAEQVMEEAT 296

BLAST of MC08g0093 vs. TAIR 10
Match: AT4G31300.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 4.1e-20
Identity = 57/185 (30.81%), Postives = 97/185 (52.43%), Query Frame = 0

Query: 55  GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 114
           GTT +   +  GV++ ADSR S G Y+++++  KI ++   +    +G AAD Q     +
Sbjct: 12  GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71

Query: 115 GVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGG 174
                +H + + +  +V  ++ L+  + Y+ + M L  G ++ GWD+   G  Y    GG
Sbjct: 72  RYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNM-LQTGLIVGGWDKYEGGKIYGIPLGG 131

Query: 175 RLKGTRFSV-GSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYY 234
            +    F++ GSGS Y YG  D  ++ +++ EEA +L  +A+  A  RDGASGGV     
Sbjct: 132 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 191

Query: 235 VGPNG 239
           +   G
Sbjct: 192 INSEG 195

BLAST of MC08g0093 vs. TAIR 10
Match: AT4G31300.3 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 4.1e-20
Identity = 57/185 (30.81%), Postives = 97/185 (52.43%), Query Frame = 0

Query: 55  GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 114
           GTT +   +  GV++ ADSR S G Y+++++  KI ++   +    +G AAD Q     +
Sbjct: 12  GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71

Query: 115 GVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGG 174
                +H + + +  +V  ++ L+  + Y+ + M L  G ++ GWD+   G  Y    GG
Sbjct: 72  RYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNM-LQTGLIVGGWDKYEGGKIYGIPLGG 131

Query: 175 RLKGTRFSV-GSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYY 234
            +    F++ GSGS Y YG  D  ++ +++ EEA +L  +A+  A  RDGASGGV     
Sbjct: 132 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 191

Query: 235 VGPNG 239
           +   G
Sbjct: 192 INSEG 195

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O243611.4e-13483.82Proteasome subunit beta type-5 OS=Spinacia oleracea OX=3562 PE=2 SV=1[more]
Q9LIP24.5e-13384.81Proteasome subunit beta type-5-B OS=Arabidopsis thaliana OX=3702 GN=PBE2 PE=1 SV... [more]
O237173.4e-12881.62Proteasome subunit beta type-5-A OS=Arabidopsis thaliana OX=3702 GN=PBE1 PE=1 SV... [more]
O552341.2e-7762.93Proteasome subunit beta type-5 OS=Mus musculus OX=10090 GN=Psmb5 PE=1 SV=3[more]
P280742.1e-7761.80Proteasome subunit beta type-5 OS=Homo sapiens OX=9606 GN=PSMB5 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
XP_022131817.19.39e-197100.00proteasome subunit beta type-5 [Momordica charantia] >XP_022131818.1 proteasome ... [more]
XP_038883961.17.11e-19297.06proteasome subunit beta type-5 isoform X1 [Benincasa hispida][more]
XP_022961789.11.01e-19196.69proteasome subunit beta type-5-like [Cucurbita moschata] >XP_022997234.1 proteas... [more]
XP_008445492.14.11e-19196.32PREDICTED: proteasome subunit beta type-5 [Cucumis melo] >KAA0064739.1 proteasom... [more]
XP_023002169.11.05e-19097.76proteasome subunit beta type-5-B isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1BS304.55e-197100.00Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111004886 PE=3 SV=1[more]
A0A6J1KAW44.89e-19296.69Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111492198 PE=3 SV=1[more]
A0A6J1HF194.89e-19296.69Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462451 PE=3 SV=1[more]
A0A5A7VGZ11.99e-19196.32Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3BCV31.99e-19196.32Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103488492 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G26340.13.2e-13484.81N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT1G13060.12.4e-12981.6220S proteasome beta subunit E1 [more]
AT1G13060.22.7e-12575.0020S proteasome beta subunit E1 [more]
AT4G31300.14.1e-2030.81N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G31300.34.1e-2030.81N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000243Peptidase T1A, proteasome beta-subunitPRINTSPR00141PROTEASOMEcoord: 195..206
score: 51.89
coord: 63..78
score: 58.24
coord: 184..195
score: 71.78
coord: 220..231
score: 53.98
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 56..263
e-value: 1.5E-74
score: 252.5
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 44..254
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 54..234
e-value: 7.5E-49
score: 165.8
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 29..271
NoneNo IPR availablePANTHERPTHR11599:SF154PROTEASOME SUBUNIT BETAcoord: 29..271
NoneNo IPR availableCDDcd03761proteasome_beta_type_5coord: 56..243
e-value: 6.6283E-132
score: 369.266
IPR016050Proteasome beta-type subunit, conserved sitePROSITEPS00854PROTEASOME_BETA_1coord: 59..106
IPR023333Proteasome B-type subunitPROSITEPS51476PROTEASOME_BETA_2coord: 55..234
score: 55.593914

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0093.1MC08g0093.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010498 proteasomal protein catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019774 proteasome core complex, beta-subunit complex
cellular_component GO:0005839 proteasome core complex
molecular_function GO:0004298 threonine-type endopeptidase activity