MC08g0073 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0073
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionHXXXD-type acyl-transferase family protein
LocationMC08: 441857 .. 443923 (-)
RNA-Seq ExpressionMC08g0073
SyntenyMC08g0073
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCTTGAAATTGTTTCGAGGGAAACCATTAAACCCTCCTCTCCAACCCCTCCCCATCTCAAAATCCATGCTCTCTCCCTCTTTGATCAGCTCGCTCCCCACATGTTCGTGCCTCTTGTTTTCTTCTTTCGCCACGATAACGACTGCAGTCGTAGTAGTATTGTCGTCATATCGGAATCTCTTAAGCTATCTCTTTCCAAGACTTTGTCTCGGTATTGTCACTGAACCCAAAAGCACCATGCTTAAATTGGTTAGTTGCATGAAGTTTAATAGGAAAATATTTGAGTCATAACTCATCAATTTTTATGAAGAGTCTCTAATGTTGCATTTCTCGCTGCGGTTAGGGAGTCTAGTATTTTCTAATTTAATATGTATTGGGTTTGAACAGGTACTACCCTTTTGCGGGACGGATGAAAGATAAAGAGCGGGTGGAGTGTAACGACGAGGGGGTAACTTTTTTGGAAGCGAGGGTGAGATCAGGGCGGCTATCGGAAGTAGTGGAGAAGCGTAGAAGTGAGATTGTGGAGGTGATGTTTGGCGATGGGTTGCAATGGAAAGAGTCAAAATCGGGTGCTTTGTTGATGGTTCAGATTACGTTATTTGAGTGTGGGGGATTTGCCGTTGGAGTGCTTCTGTCCCATAAGCTGGCGGATATGGCCACCATGGTCAACTTCATCCAAGACTGGGCTTTGATTTCTCGCGATGCCGCTTTGGGAGAACAACTTGTGAATCCACTGTTCGTCGCCGCCGATTTGTTTCCTCTTGGCGATTTGCCGGCCATGTCCGGCGCCGTTATTGAAGACGGAAAAGGCTTCCTATGTCATAGGTCTGTGAGAATGGAAGTTAGATCCTTTAATCTTTTAGCCCATGATTGAGTTAATTAAACCATTATCATAGACTAAGTTTGATTGGGTATGCCTAATTTAAGATTGAATGATAGTTAACCATGATTGGTCAGTCATGGTCACTGTTTTTAATCTAGAGAAAGGAAGGGAAAAAAAAAGAGATTTTAGCGATTGTACTTTTTAAAAAATTTACTTCAAACATTTAAATTTTTAATTTTATTTAATTTTTTTCCCAAACTATTCAAAATATGTATTTCAGTGATTCAACTTTTTAAAAAATATTGTTAGTCATCTTGATCTTTTTTTTTTTTTTTTCATTTTTAAGAACTAGATAATACAGTGTATTGTGATGTTGTACTAACATGAACATATATTAGTCATATATGTTAGAATGAAATTAGAACGGGGTGATAACGATTGAAATAATTTCTTAGAGTTCAAACCGAAAATAAAAAAATAGAAAAATTTAGAAAGTTAGGGTCGATTTTTATTTAGAACAATCAAAATATGATTTAAAACTAAATTTAATAATTTATTTATATTCGAATTTGGCTATAAAAAAAATGCTTAACAGTTTATTGATATTGTTGACAGAATTGTGTTCGAAGGTTCAAAGATCGAAGCGCTCAAAAACTCAGTTTCCGACAAAGTGGAGAATCCAAGCCGCGTGGAAGTTGTGTCCGCATTGATTTACAAAACCCTGATTTCAGCATCCCCTTCCCCTTCCAATTCATTAATGCTGCAGACGCTAAATTTACGCACACGAGTGGCGCCGCCGCTGCCGGAAAATGTAGTCGGAAGTTTGGTGTCGTTCTTCCCGGTGGCGGCCGGCGGGGAGAAAGAGGTGGAATTACACGAGCTGGTGGGGAAAATGAGGGACGAAATGGCAGATTTCTGCAACAAATACGCCAAAAAGTACAGAACAGAGGAGTGGGGCGAGGCGATAAAGAAGCGATTGAAGGAATCGGGAGAAATTCTGACCAAAAATGGTGGAAACCAAGAGGTGTACAGATTCAGCAGTGGGTGCAGATTTCCGATTTATGAAGTGGATTTTGGGTGGGGAGCGGCGGCGTGGGTCACCATGGCGGCGTTCCGGCTGAAGAATACGGTGATGATGTTGGACGCCAAAAGCGGCGGCGGAATTGAGGCCTTGGTGGGATTGGGGCACAACCAAATGGCTGCGTTTCTGCAAAATCAAGAGCTTTTATCCTTTGCTTCC

mRNA sequence

ATGTTGCTTGAAATTGTTTCGAGGGAAACCATTAAACCCTCCTCTCCAACCCCTCCCCATCTCAAAATCCATGCTCTCTCCCTCTTTGATCAGCTCGCTCCCCACATGTTCGTGCCTCTTGTTTTCTTCTTTCGCCACGATAACGACTGCAGTCGTAGTAGTATTGTCGTCATATCGGAATCTCTTAAGCTATCTCTTTCCAAGACTTTGTCTCGGTACTACCCTTTTGCGGGACGGATGAAAGATAAAGAGCGGGTGGAGTGTAACGACGAGGGGGTAACTTTTTTGGAAGCGAGGGTGAGATCAGGGCGGCTATCGGAAGTAGTGGAGAAGCGTAGAAGTGAGATTGTGGAGGTGATGTTTGGCGATGGGTTGCAATGGAAAGAGTCAAAATCGGGTGCTTTGTTGATGGTTCAGATTACGTTATTTGAGTGTGGGGGATTTGCCGTTGGAGTGCTTCTGTCCCATAAGCTGGCGGATATGGCCACCATGGTCAACTTCATCCAAGACTGGGCTTTGATTTCTCGCGATGCCGCTTTGGGAGAACAACTTGTGAATCCACTGTTCGTCGCCGCCGATTTGTTTCCTCTTGGCGATTTGCCGGCCATGTCCGGCGCCGTTATTGAAGACGGAAAAGGCTTCCTATGTCATAGAATTGTGTTCGAAGGTTCAAAGATCGAAGCGCTCAAAAACTCAGTTTCCGACAAAGTGGAGAATCCAAGCCGCGTGGAAGTTGTGTCCGCATTGATTTACAAAACCCTGATTTCAGCATCCCCTTCCCCTTCCAATTCATTAATGCTGCAGACGCTAAATTTACGCACACGAGTGGCGCCGCCGCTGCCGGAAAATGTAGTCGGAAGTTTGGTGTCGTTCTTCCCGGTGGCGGCCGGCGGGGAGAAAGAGGTGGAATTACACGAGCTGGTGGGGAAAATGAGGGACGAAATGGCAGATTTCTGCAACAAATACGCCAAAAAGTACAGAACAGAGGAGTGGGGCGAGGCGATAAAGAAGCGATTGAAGGAATCGGGAGAAATTCTGACCAAAAATGGTGGAAACCAAGAGGTGTACAGATTCAGCAGTGGGTGCAGATTTCCGATTTATGAAGTGGATTTTGGGTGGGGAGCGGCGGCGTGGGTCACCATGGCGGCGTTCCGGCTGAAGAATACGGTGATGATGTTGGACGCCAAAAGCGGCGGCGGAATTGAGGCCTTGGTGGGATTGGGGCACAACCAAATGGCTGCGTTTCTGCAAAATCAAGAGCTTTTATCCTTTGCTTCC

Coding sequence (CDS)

ATGTTGCTTGAAATTGTTTCGAGGGAAACCATTAAACCCTCCTCTCCAACCCCTCCCCATCTCAAAATCCATGCTCTCTCCCTCTTTGATCAGCTCGCTCCCCACATGTTCGTGCCTCTTGTTTTCTTCTTTCGCCACGATAACGACTGCAGTCGTAGTAGTATTGTCGTCATATCGGAATCTCTTAAGCTATCTCTTTCCAAGACTTTGTCTCGGTACTACCCTTTTGCGGGACGGATGAAAGATAAAGAGCGGGTGGAGTGTAACGACGAGGGGGTAACTTTTTTGGAAGCGAGGGTGAGATCAGGGCGGCTATCGGAAGTAGTGGAGAAGCGTAGAAGTGAGATTGTGGAGGTGATGTTTGGCGATGGGTTGCAATGGAAAGAGTCAAAATCGGGTGCTTTGTTGATGGTTCAGATTACGTTATTTGAGTGTGGGGGATTTGCCGTTGGAGTGCTTCTGTCCCATAAGCTGGCGGATATGGCCACCATGGTCAACTTCATCCAAGACTGGGCTTTGATTTCTCGCGATGCCGCTTTGGGAGAACAACTTGTGAATCCACTGTTCGTCGCCGCCGATTTGTTTCCTCTTGGCGATTTGCCGGCCATGTCCGGCGCCGTTATTGAAGACGGAAAAGGCTTCCTATGTCATAGAATTGTGTTCGAAGGTTCAAAGATCGAAGCGCTCAAAAACTCAGTTTCCGACAAAGTGGAGAATCCAAGCCGCGTGGAAGTTGTGTCCGCATTGATTTACAAAACCCTGATTTCAGCATCCCCTTCCCCTTCCAATTCATTAATGCTGCAGACGCTAAATTTACGCACACGAGTGGCGCCGCCGCTGCCGGAAAATGTAGTCGGAAGTTTGGTGTCGTTCTTCCCGGTGGCGGCCGGCGGGGAGAAAGAGGTGGAATTACACGAGCTGGTGGGGAAAATGAGGGACGAAATGGCAGATTTCTGCAACAAATACGCCAAAAAGTACAGAACAGAGGAGTGGGGCGAGGCGATAAAGAAGCGATTGAAGGAATCGGGAGAAATTCTGACCAAAAATGGTGGAAACCAAGAGGTGTACAGATTCAGCAGTGGGTGCAGATTTCCGATTTATGAAGTGGATTTTGGGTGGGGAGCGGCGGCGTGGGTCACCATGGCGGCGTTCCGGCTGAAGAATACGGTGATGATGTTGGACGCCAAAAGCGGCGGCGGAATTGAGGCCTTGGTGGGATTGGGGCACAACCAAATGGCTGCGTTTCTGCAAAATCAAGAGCTTTTATCCTTTGCTTCC

Protein sequence

MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISESLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVMFGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAALGEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVENPSRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQNQELLSFAS
Homology
BLAST of MC08g0073 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.7e-65
Identity = 151/436 (34.63%), Postives = 247/436 (56.65%), Query Frame = 0

Query: 2   LLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFF-----RHDNDCSRSSIV 61
           L+ + ++  IKPSS TP  L+ H LS  DQ   +M++P  FF+     R ++  +   + 
Sbjct: 9   LVSVCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELS 68

Query: 62  VISESLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEI 121
            I+  L+ SLS+TL  YYP+AG++KD   V+CND G  FL  R++   +SE+++   + +
Sbjct: 69  HIAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCS-MSEILDHPHASL 128

Query: 122 VE-VMFGDGLQWKES-KSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALI 181
            E ++    L W  + + G LL+VQ++ F+CGG A+ V  SHK+ D  +++NF+ DW+ +
Sbjct: 129 AESIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSV 188

Query: 182 SRDAALGEQLVNPLFVAADLFPLGDLPAM-SGAVIEDGKGFLCHRIVFEGSKIEALKNSV 241
           +RD      + +P FV   +F      ++ +  ++ D    +  R++F   K++AL+  V
Sbjct: 189 TRDRTTTTLVPSPRFVGDSVFSTQKYGSLITPQILSDLNQCVQKRLIFPTDKLDALRAKV 248

Query: 242 SDK--VENPSRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSF 301
           +++  V+NP+R EVVSAL++K    AS S   S ++  LN+RT + P LP N +G+L S 
Sbjct: 249 AEESGVKNPTRAEVVSALLFKCATKASSSMLPSKLVHFLNIRTMIKPRLPRNAIGNLSSI 308

Query: 302 FPVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLK--ESGEILTKN 361
           F + A   +++EL  LV  +R E+     + A K    E  E I + ++    G++  +N
Sbjct: 309 FSIEATNMQDMELPTLVRNLRKEV-----EVAYKKDQVEQNELILEVVESMREGKLPFEN 368

Query: 362 -GGNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLG 421
             G + VY  S+ C++P Y VDFGWG    V +     KN   + D K+G G+EA V L 
Sbjct: 369 MDGYKNVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVEARVMLH 428

Query: 422 HNQMAAFLQNQELLSF 425
             QM+ F +N+ELL F
Sbjct: 429 KQQMSEFERNEELLEF 438

BLAST of MC08g0073 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.7e-65
Identity = 152/436 (34.86%), Postives = 246/436 (56.42%), Query Frame = 0

Query: 2   LLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFF-----RHDNDCSRSSIV 61
           L+ I  +  IKPSS TP  L+ H LS  DQ   +M++P  FF+     R ++  +   + 
Sbjct: 9   LVSICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELS 68

Query: 62  VISESLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEI 121
            I+  L+ SLS+TL  YYP+AG++KD   V+CND G  FL  R++   +SE+++   + +
Sbjct: 69  HIAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCS-MSEILDHPHASL 128

Query: 122 VE-VMFGDGLQWKES-KSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALI 181
            E ++    L W  + + G LL+VQ++ F+CGG A+ V  SHK+ D  +++NF+ DW+ +
Sbjct: 129 AESIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSV 188

Query: 182 SRDAALGEQLVNPLFVAADLFPLGDLPAM-SGAVIEDGKGFLCHRIVFEGSKIEALKNSV 241
           +RD      + +P FV   +F      ++ +  ++ D    +  R++F   K++AL+  V
Sbjct: 189 TRDHTTTTLVPSPRFVGDSVFSTKKYGSLITPQILSDLNECVQKRLIFPTDKLDALRAKV 248

Query: 242 SDK--VENPSRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSF 301
           +++  V+NP+R EVVSAL++K    AS S   S ++  LN+RT + P LP N +G+L S 
Sbjct: 249 AEESGVKNPTRAEVVSALLFKCATKASSSMLPSKLVHFLNIRTMIKPRLPRNAIGNLSSI 308

Query: 302 FPVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLK--ESGEILTKN 361
           F + A   +++EL  LV  +R E+     + A K    E  E I + ++    G++  +N
Sbjct: 309 FSIEATNMQDMELPTLVRNLRKEV-----EVAYKKDQVEQNELILEVVESMREGKLPFEN 368

Query: 362 -GGNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLG 421
             G + VY  S+ C++P Y VDFGWG    V +     KN   + D K+G G+EA V L 
Sbjct: 369 MDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVEARVMLH 428

Query: 422 HNQMAAFLQNQELLSF 425
             QM+ F +N+ELL F
Sbjct: 429 KQQMSEFERNEELLEF 438

BLAST of MC08g0073 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.2e-65
Identity = 151/436 (34.63%), Postives = 246/436 (56.42%), Query Frame = 0

Query: 2   LLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFF-----RHDNDCSRSSIV 61
           L+ +  +  IKPSS TP  L+ H LS  DQ   +M++P  FF+     R ++  +   + 
Sbjct: 9   LVSVCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELS 68

Query: 62  VISESLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEI 121
            I+  L+ SLS+TL  YYP+AG++KD   V+CND G  FL  R++   +SE+++   + +
Sbjct: 69  HIAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCS-MSEILDHPHASL 128

Query: 122 VE-VMFGDGLQWKES-KSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALI 181
            E ++    L W  + + G LL+VQ++ F+CGG A+ V  SHK+ D  +++NF+ DW+ +
Sbjct: 129 AESIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSV 188

Query: 182 SRDAALGEQLVNPLFVAADLFPLGDLPAM-SGAVIEDGKGFLCHRIVFEGSKIEALKNSV 241
           +RD      + +P FV   +F      ++ +  ++ D    +  R++F   K++AL+  V
Sbjct: 189 TRDHTTTTLVPSPRFVGDSVFSTKTYGSLTTPQILSDLNECVQKRLIFPTDKLDALRAKV 248

Query: 242 SDK--VENPSRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSF 301
           +++  V+NP+R EVVSAL++K    AS S   S ++  LN+RT + P LP N +G+L S 
Sbjct: 249 AEESGVKNPTRAEVVSALLFKCATKASSSMLPSKLVHFLNIRTMIKPRLPRNTIGNLSSI 308

Query: 302 FPVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLK--ESGEILTKN 361
           F + A   +++EL  LV  +R E+     + A K    E  E I + ++    G++  +N
Sbjct: 309 FSIEATNMQDMELPTLVRNLRKEV-----EVAYKKDQVEQNELILEVVESMREGKLPFEN 368

Query: 362 -GGNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLG 421
             G + VY  S+ C++P Y VDFGWG    V +     KN   + D K+G G+EA V L 
Sbjct: 369 MDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVEARVMLH 428

Query: 422 HNQMAAFLQNQELLSF 425
             QM+ F +N+ELL F
Sbjct: 429 KQQMSEFERNEELLEF 438

BLAST of MC08g0073 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 9.2e-56
Identity = 143/427 (33.49%), Postives = 233/427 (54.57%), Query Frame = 0

Query: 2   LLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISES 61
           ++EIVS+  IKPSSPTP  L+ + LS  DQ        L FF+ ++     S    I + 
Sbjct: 5   MVEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSK---IGDL 64

Query: 62  LKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVMF 121
           LK SLSKTL  YY FAGR+ + + ++CNDEGV F+E R+  GR+++++++ +S   +++ 
Sbjct: 65  LKNSLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIH-GRMNDILKRGKSFATDLVL 124

Query: 122 GDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAALG 181
              +      S  LL+VQ++ F+CGG A+G   SHK++D  + V F++DWA  S  +   
Sbjct: 125 PTRIIALHEDS--LLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWA--SSTSLST 184

Query: 182 EQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKN-SVSDKVENP 241
                PL  A  +FP  D   +S   I   +  L  R VF    IE LK+ ++   ++ P
Sbjct: 185 FHKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEYGIQKP 244

Query: 242 SRVEVVSALIYKTLISAS-PSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGGE 301
           SRVEVV+A + +   +   P      ++  +NLR  +A  LP+N +G++ SF+       
Sbjct: 245 SRVEVVTAFLCQCAANCDLPRKKPYAIISAVNLRPYLA--LPQNSIGNIFSFYFCINDEG 304

Query: 302 KEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTK-NGGNQEVYRF 361
            + +   L+ K+R+           K +        + +++E  + L + N  + + Y  
Sbjct: 305 MDNQFSALISKLRNGKQKLLENIISKEKL-----TYESQMQELTKCLDQLNISSLDTYFC 364

Query: 362 SSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQN 421
           SS CRFP+Y++DFGWG    V+     +K+ ++++D+  G GIEAL+ +   +MAAF +N
Sbjct: 365 SSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALITMEEKKMAAFEKN 416

Query: 422 QELLSFA 426
           +EL SFA
Sbjct: 425 EELRSFA 416

BLAST of MC08g0073 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.8e-52
Identity = 137/424 (32.31%), Postives = 227/424 (53.54%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISESL 62
           ++I+S E I+PSSPTP  LK H LS  DQ+     +P++ F+ +  D S       SE+L
Sbjct: 5   MQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLD-SNLDRAQRSENL 64

Query: 63  KLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEK----RRSEIVE 122
           K SLS  L+++YP AGR+     V+CND GV FLEARV S +LSE ++       ++ + 
Sbjct: 65  KRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHS-QLSEAIKNVAIDELNQYLP 124

Query: 123 VMFGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDA 182
                G +    K    L V+I+ FECGG A+GV +SHK+AD  ++  F+  W    ++ 
Sbjct: 125 FQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQEE 184

Query: 183 ALGEQLVNPLF-VAADLF-PLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALK---NSV 242
                +V P F + +  F P+  +PA      E+    +  R VF+  K+EALK    S 
Sbjct: 185 T---DIVQPNFDLGSHHFPPMESIPAPEFLPDEN---IVMKRFVFDKEKLEALKAQLASS 244

Query: 243 SDKVENPSRVEVVSALIYKTLISAS----PSPSNSLMLQTLNLRTRVAPPLPENVVGSLV 302
           + +V+N SRV++V A+I+K  I  +     + +  +  Q +NLR+R+ PP P++ +G++ 
Sbjct: 245 ATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAAQAVNLRSRMNPPFPQSAMGNIA 304

Query: 303 SFFPVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKN 362
           +     A  E++ +  +LVG ++  +A   +++ K+             L++    L   
Sbjct: 305 TM--AYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKE-------------LQKGVTYLDYE 364

Query: 363 GGNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGH 414
              QE++ FSS CR   Y++DFGWG    V      +KN V ++D ++  G+EA + +  
Sbjct: 365 AEPQELFSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAE 405

BLAST of MC08g0073 vs. NCBI nr
Match: XP_022132095.1 (BAHD acyltransferase At5g47980-like [Momordica charantia])

HSP 1 Score: 837 bits (2162), Expect = 9.11e-306
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60
           MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE
Sbjct: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60

Query: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM 120
           SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM
Sbjct: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM 120

Query: 121 FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL 180
           FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL
Sbjct: 121 FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL 180

Query: 181 GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVENP 240
           GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVENP
Sbjct: 181 GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVENP 240

Query: 241 SRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGGEK 300
           SRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGGEK
Sbjct: 241 SRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGGEK 300

Query: 301 EVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGNQEVYRFSS 360
           EVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGNQEVYRFSS
Sbjct: 301 EVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGNQEVYRFSS 360

Query: 361 GCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQNQE 420
           GCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQNQE
Sbjct: 361 GCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQNQE 420

Query: 421 LLSFA 425
           LLSFA
Sbjct: 421 LLSFA 425

BLAST of MC08g0073 vs. NCBI nr
Match: XP_038886275.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 590 bits (1520), Expect = 7.51e-208
Identity = 304/429 (70.86%), Postives = 354/429 (82.52%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFF-RHDNDCS-RSSIVVISE 62
           L+IVS ETIKP SPTPP+L IH LSLFDQLAPHMFVPLVFFF RH  D       V  S+
Sbjct: 9   LQIVSSETIKPYSPTPPNLNIHTLSLFDQLAPHMFVPLVFFFSRHGPDLGINGHDVDRSK 68

Query: 63  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM 122
            L+ SLS TLSRYYPFAGR+KD   V+ NDEGV+F+EA+V    +SE++EK RSEIVE++
Sbjct: 69  LLRRSLSMTLSRYYPFAGRIKDNVSVDSNDEGVSFVEAQVEGVTVSEILEKPRSEIVEML 128

Query: 123 FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL 182
           F DGLQWKESK GALL VQITLFECGG ++GVLLSHKL D+AT+V F+QDWA+I+R+   
Sbjct: 129 FVDGLQWKESKVGALLKVQITLFECGGLSIGVLLSHKLGDLATLVKFVQDWAVITRNGGF 188

Query: 183 GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVENP 242
           GE++VNPLF +ADLFP GDLPAMSGAVIE+G  F C R +FEGSKIE+LKN VS+K+ENP
Sbjct: 189 GEEIVNPLFYSADLFPHGDLPAMSGAVIEEGN-FTCKRFLFEGSKIESLKNRVSEKMENP 248

Query: 243 SRVEVVSALIYKTLISASPSPSN--SLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGG 302
           SRVEVVSALIYK +I+A+ +  N  +L+LQTLNLR RVAPPLPE++VGSLVSFFPV   G
Sbjct: 249 SRVEVVSALIYKAIIAATRNSQNHPTLLLQTLNLRKRVAPPLPESLVGSLVSFFPVGVAG 308

Query: 303 EKEV-ELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGNQEVYR 362
           E EV ELHELVG MR EM +FCNKYAKKYRT+EW + IKKRL ES EIL+KNG NQ +YR
Sbjct: 309 ETEVIELHELVGIMRKEMGEFCNKYAKKYRTKEWHDLIKKRLNESREILSKNGNNQLIYR 368

Query: 363 FSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQ 422
           FSSGC FPIYEVDFGWGAA WVTMAAF++KNTVMMLDAK+GGGIEALV L   +M AF  
Sbjct: 369 FSSGCNFPIYEVDFGWGAAGWVTMAAFKMKNTVMMLDAKNGGGIEALVSLQDKEMTAFQH 428

Query: 423 NQELLSFAS 426
           N+E+L+FAS
Sbjct: 429 NEEILAFAS 436

BLAST of MC08g0073 vs. NCBI nr
Match: XP_008444410.1 (PREDICTED: vinorine synthase [Cucumis melo])

HSP 1 Score: 568 bits (1463), Expect = 4.31e-199
Identity = 296/436 (67.89%), Postives = 348/436 (79.82%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRS-----SIVV 62
           L+IVS + IKPSSPTPP+L IH LSLFDQLAPH FVPLVFFF H     RS     + V 
Sbjct: 9   LQIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLVFFFSHQGP-GRSGTYYHTTVD 68

Query: 63  ISESLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIV 122
             E L+ SLS TLSRYYPFAGR+KDK  ++CNDEGVTF+EA +    +S+++E  RSEIV
Sbjct: 69  KLELLRQSLSTTLSRYYPFAGRIKDKVSIDCNDEGVTFVEAWLEGVTVSKILENPRSEIV 128

Query: 123 EVMFGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRD 182
           E++F DGLQWK+SK G+LL VQITLFECGG ++GVLLSHKL D+AT+V F+QDW LI+R+
Sbjct: 129 EMLFVDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFVQDWGLITRN 188

Query: 183 AAL-GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDK 242
                E+ VNPLF +AD+FP GDLPAMSGAVIE+G  F C R VFEGSKIE+LKN +S+K
Sbjct: 189 NGFCEEETVNPLFFSADVFPHGDLPAMSGAVIEEGN-FTCKRFVFEGSKIESLKNRISEK 248

Query: 243 VENPSRVEVVSALIYKTLISASPSPSNS-----LMLQTLNLRTRVAPPLPENVVGSLVSF 302
           V+NPSRVEVVSALIYK +ISAS + +NS     L+LQTLNLR RV PPLPE +VGSLVSF
Sbjct: 249 VQNPSRVEVVSALIYKAIISASRNNNNSQNQSTLLLQTLNLRKRVVPPLPETLVGSLVSF 308

Query: 303 FPVAAGGEKEV-ELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNG 362
           FPV   GE+EV ELHELVG MR EM +FC++YAKKYRT+EW E IKKRL ES EIL+KNG
Sbjct: 309 FPVGVDGEREVVELHELVGTMRKEMGEFCDRYAKKYRTKEWLELIKKRLNESREILSKNG 368

Query: 363 GNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHN 422
            NQ VYR SSGC FPIYEVDFGWG A W+TM AF++KNTVMMLDAK+GGGIEAL+ L   
Sbjct: 369 NNQLVYRCSSGCNFPIYEVDFGWGVADWITMPAFKMKNTVMMLDAKNGGGIEALISLQDG 428

Query: 423 QMAAFLQNQELLSFAS 426
           +MAAF  N+ELL+FAS
Sbjct: 429 EMAAFQDNEELLAFAS 442

BLAST of MC08g0073 vs. NCBI nr
Match: XP_023538329.1 (acylsugar acyltransferase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 568 bits (1463), Expect = 7.35e-199
Identity = 293/436 (67.20%), Postives = 350/436 (80.28%), Query Frame = 0

Query: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60
           M L+IVSR++IKPSSPTPPHLK HALSLFDQLAPHM+VPLVFFFR  +  S +     S 
Sbjct: 16  MQLQIVSRQSIKPSSPTPPHLKTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCT-SH 75

Query: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSG-RLSEVVEKRRSEIVEV 120
            L+ SLS TLSRYYPFAGR+KDK  V+CNDEGVTFLEAR+     L+E++E  +SEIVE+
Sbjct: 76  FLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLGGNVTLAEILEGPKSEIVEM 135

Query: 121 MFGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAA 180
           +F DGLQWK SK G LL +Q+T F+CGGFAVGVLLSHKL D+AT+V F+ DW  I+RD  
Sbjct: 136 LFVDGLQWKHSKMGPLLAIQVTYFQCGGFAVGVLLSHKLGDLATLVKFVTDWGFITRDTT 195

Query: 181 LGEQ-LVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVE 240
              Q  VNPLF +ADLFPLGDLP MS AVIE+GK   C R VFE SKIEALK S+SDKV+
Sbjct: 196 TSAQHTVNPLFHSADLFPLGDLPPMSSAVIEEGKNLTCKRFVFEASKIEALKRSISDKVK 255

Query: 241 NPSRVEVVSALIYKTLISA----SPSPSN--SLMLQTLNLRTRVAPPLPENVVGSLVSFF 300
           NP+RVEVV+ALIYK +ISA    + +P+N  +L+LQT+NLRTR+ PPLPE+ +G++VSFF
Sbjct: 256 NPTRVEVVTALIYKAIISALRDSNSNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFF 315

Query: 301 PVAAG--GEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNG 360
           PVAA   GE+EVE+HE+VG MR EM +FC+KYAKKYRTEEW E IKKRLKE  EIL+KNG
Sbjct: 316 PVAAAASGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLKEVREILSKNG 375

Query: 361 GNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHN 420
             Q +YR SSGC FP+Y VDFGWGAAAWVTMAAF++KNTVMMLD K+GGGIEAL+ L   
Sbjct: 376 KPQLIYRCSSGCNFPVYGVDFGWGAAAWVTMAAFKMKNTVMMLDDKNGGGIEALLSLDDK 435

Query: 421 QMAAFLQNQELLSFAS 426
           +MAAF  N+ELL++AS
Sbjct: 436 EMAAFQHNEELLAYAS 450

BLAST of MC08g0073 vs. NCBI nr
Match: XP_004143047.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62352.1 hypothetical protein Csa_018554 [Cucumis sativus])

HSP 1 Score: 566 bits (1459), Expect = 1.69e-198
Identity = 294/434 (67.74%), Postives = 346/434 (79.72%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVIS-ES 62
           L+IVS + IKPSSPTPP+L IH LSLFDQLAPH FVPL+FFF H    S +     + E 
Sbjct: 9   LQIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLLFFFSHHGPSSGTYYDHTTVEL 68

Query: 63  LKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVMF 122
           L+ SLS TLSRYYP AGR+KDK  ++CNDEGVTF+EAR+    +S+++E  RSEIVE +F
Sbjct: 69  LRQSLSTTLSRYYPLAGRIKDKVSIDCNDEGVTFVEARLEGVTVSKILENPRSEIVEKLF 128

Query: 123 GDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL- 182
            DGLQWK+SK G+LL VQITLFECGG ++GVLLSHKL D+AT+V FIQDW +I+R+    
Sbjct: 129 VDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFIQDWGVITRNNGFC 188

Query: 183 -GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVEN 242
             E+++NPLF + DLFP GDLPAMSGAVIE+G  F C R VFEGSKIE+LKN +S+KV+N
Sbjct: 189 DEEEIINPLFFSGDLFPHGDLPAMSGAVIEEGN-FTCKRFVFEGSKIESLKNRISEKVQN 248

Query: 243 PSRVEVVSALIYKTLISASPSPSNS------LMLQTLNLRTRVAPPLPENVVGSLVSFFP 302
           PSRVEVVSALIYK +ISAS + +NS      L+LQTLNLR RVAPPLP+ +VGSLVSFFP
Sbjct: 249 PSRVEVVSALIYKAIISASRTNNNSQNHPATLLLQTLNLRKRVAPPLPDTLVGSLVSFFP 308

Query: 303 VAAGGEKEV-ELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGN 362
           V   GE+EV ELHELVG MR EM +FC KYAKKYRT+EW E IKKRL ES EIL+KNG N
Sbjct: 309 VGVDGEREVVELHELVGTMRKEMGEFCEKYAKKYRTKEWLELIKKRLNESREILSKNGNN 368

Query: 363 QEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQM 422
           Q VYR SSGC FPIYEVDFGWGAA W+TM AF++KNTVMMLDAK+G GIEALV L   +M
Sbjct: 369 QLVYRCSSGCNFPIYEVDFGWGAADWITMPAFKMKNTVMMLDAKNGRGIEALVSLQDGEM 428

Query: 423 AAFLQNQELLSFAS 426
           AAF  N+ELL+FAS
Sbjct: 429 AAFQDNEELLAFAS 441

BLAST of MC08g0073 vs. ExPASy TrEMBL
Match: A0A6J1BRH3 (BAHD acyltransferase At5g47980-like OS=Momordica charantia OX=3673 GN=LOC111005052 PE=3 SV=1)

HSP 1 Score: 837 bits (2162), Expect = 4.41e-306
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60
           MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE
Sbjct: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60

Query: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM 120
           SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM
Sbjct: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM 120

Query: 121 FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL 180
           FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL
Sbjct: 121 FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL 180

Query: 181 GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVENP 240
           GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVENP
Sbjct: 181 GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVENP 240

Query: 241 SRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGGEK 300
           SRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGGEK
Sbjct: 241 SRVEVVSALIYKTLISASPSPSNSLMLQTLNLRTRVAPPLPENVVGSLVSFFPVAAGGEK 300

Query: 301 EVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGNQEVYRFSS 360
           EVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGNQEVYRFSS
Sbjct: 301 EVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGNQEVYRFSS 360

Query: 361 GCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQNQE 420
           GCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQNQE
Sbjct: 361 GCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQMAAFLQNQE 420

Query: 421 LLSFA 425
           LLSFA
Sbjct: 421 LLSFA 425

BLAST of MC08g0073 vs. ExPASy TrEMBL
Match: A0A1S3BB28 (vinorine synthase OS=Cucumis melo OX=3656 GN=LOC103487747 PE=3 SV=1)

HSP 1 Score: 568 bits (1463), Expect = 2.09e-199
Identity = 296/436 (67.89%), Postives = 348/436 (79.82%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRS-----SIVV 62
           L+IVS + IKPSSPTPP+L IH LSLFDQLAPH FVPLVFFF H     RS     + V 
Sbjct: 9   LQIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLVFFFSHQGP-GRSGTYYHTTVD 68

Query: 63  ISESLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIV 122
             E L+ SLS TLSRYYPFAGR+KDK  ++CNDEGVTF+EA +    +S+++E  RSEIV
Sbjct: 69  KLELLRQSLSTTLSRYYPFAGRIKDKVSIDCNDEGVTFVEAWLEGVTVSKILENPRSEIV 128

Query: 123 EVMFGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRD 182
           E++F DGLQWK+SK G+LL VQITLFECGG ++GVLLSHKL D+AT+V F+QDW LI+R+
Sbjct: 129 EMLFVDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFVQDWGLITRN 188

Query: 183 AAL-GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDK 242
                E+ VNPLF +AD+FP GDLPAMSGAVIE+G  F C R VFEGSKIE+LKN +S+K
Sbjct: 189 NGFCEEETVNPLFFSADVFPHGDLPAMSGAVIEEGN-FTCKRFVFEGSKIESLKNRISEK 248

Query: 243 VENPSRVEVVSALIYKTLISASPSPSNS-----LMLQTLNLRTRVAPPLPENVVGSLVSF 302
           V+NPSRVEVVSALIYK +ISAS + +NS     L+LQTLNLR RV PPLPE +VGSLVSF
Sbjct: 249 VQNPSRVEVVSALIYKAIISASRNNNNSQNQSTLLLQTLNLRKRVVPPLPETLVGSLVSF 308

Query: 303 FPVAAGGEKEV-ELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNG 362
           FPV   GE+EV ELHELVG MR EM +FC++YAKKYRT+EW E IKKRL ES EIL+KNG
Sbjct: 309 FPVGVDGEREVVELHELVGTMRKEMGEFCDRYAKKYRTKEWLELIKKRLNESREILSKNG 368

Query: 363 GNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHN 422
            NQ VYR SSGC FPIYEVDFGWG A W+TM AF++KNTVMMLDAK+GGGIEAL+ L   
Sbjct: 369 NNQLVYRCSSGCNFPIYEVDFGWGVADWITMPAFKMKNTVMMLDAKNGGGIEALISLQDG 428

Query: 423 QMAAFLQNQELLSFAS 426
           +MAAF  N+ELL+FAS
Sbjct: 429 EMAAFQDNEELLAFAS 442

BLAST of MC08g0073 vs. ExPASy TrEMBL
Match: A0A0A0LNF8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350350 PE=3 SV=1)

HSP 1 Score: 566 bits (1459), Expect = 8.16e-199
Identity = 294/434 (67.74%), Postives = 346/434 (79.72%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVIS-ES 62
           L+IVS + IKPSSPTPP+L IH LSLFDQLAPH FVPL+FFF H    S +     + E 
Sbjct: 9   LQIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLLFFFSHHGPSSGTYYDHTTVEL 68

Query: 63  LKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVMF 122
           L+ SLS TLSRYYP AGR+KDK  ++CNDEGVTF+EAR+    +S+++E  RSEIVE +F
Sbjct: 69  LRQSLSTTLSRYYPLAGRIKDKVSIDCNDEGVTFVEARLEGVTVSKILENPRSEIVEKLF 128

Query: 123 GDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL- 182
            DGLQWK+SK G+LL VQITLFECGG ++GVLLSHKL D+AT+V FIQDW +I+R+    
Sbjct: 129 VDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFIQDWGVITRNNGFC 188

Query: 183 -GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVEN 242
             E+++NPLF + DLFP GDLPAMSGAVIE+G  F C R VFEGSKIE+LKN +S+KV+N
Sbjct: 189 DEEEIINPLFFSGDLFPHGDLPAMSGAVIEEGN-FTCKRFVFEGSKIESLKNRISEKVQN 248

Query: 243 PSRVEVVSALIYKTLISASPSPSNS------LMLQTLNLRTRVAPPLPENVVGSLVSFFP 302
           PSRVEVVSALIYK +ISAS + +NS      L+LQTLNLR RVAPPLP+ +VGSLVSFFP
Sbjct: 249 PSRVEVVSALIYKAIISASRTNNNSQNHPATLLLQTLNLRKRVAPPLPDTLVGSLVSFFP 308

Query: 303 VAAGGEKEV-ELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGN 362
           V   GE+EV ELHELVG MR EM +FC KYAKKYRT+EW E IKKRL ES EIL+KNG N
Sbjct: 309 VGVDGEREVVELHELVGTMRKEMGEFCEKYAKKYRTKEWLELIKKRLNESREILSKNGNN 368

Query: 363 QEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQM 422
           Q VYR SSGC FPIYEVDFGWGAA W+TM AF++KNTVMMLDAK+G GIEALV L   +M
Sbjct: 369 QLVYRCSSGCNFPIYEVDFGWGAADWITMPAFKMKNTVMMLDAKNGRGIEALVSLQDGEM 428

Query: 423 AAFLQNQELLSFAS 426
           AAF  N+ELL+FAS
Sbjct: 429 AAFQDNEELLAFAS 441

BLAST of MC08g0073 vs. ExPASy TrEMBL
Match: A0A5A7V5H2 (Vinorine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001670 PE=3 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 5.87e-196
Identity = 293/436 (67.20%), Postives = 345/436 (79.13%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRS-----SIVV 62
           L+IVS + IKPSSPTPP+L IH LSLFDQLAPH FVPLVFFF H     RS     + V 
Sbjct: 9   LQIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLVFFFSHQGP-GRSGTYYHTTVD 68

Query: 63  ISESLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIV 122
             E L+ SLS TLSRYYPFAGR+KDK  ++CNDEGVTF+EA +    +S+++E  RSEIV
Sbjct: 69  KLELLRQSLSTTLSRYYPFAGRIKDKVSIDCNDEGVTFVEAWLEGVTVSKILENPRSEIV 128

Query: 123 EVMFGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRD 182
           E++F DGLQWK+SK G+LL VQITLFECGG ++GVLLSHKL D+AT+V F+QDW LI+R+
Sbjct: 129 EMLFVDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFVQDWGLITRN 188

Query: 183 AAL-GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDK 242
                E+ VNPLF +AD+FP GDLPAMSGAVIE+G        VFEGSKIE+LKN +S+K
Sbjct: 189 NGFCEEETVNPLFFSADVFPHGDLPAMSGAVIEEGN----FTFVFEGSKIESLKNRISEK 248

Query: 243 VENPSRVEVVSALIYKTLISASPSPSNS-----LMLQTLNLRTRVAPPLPENVVGSLVSF 302
           V+NPSRVEVVSALIYK +ISAS + +NS     L+LQTLNLR RV PPLPE +VGSLVSF
Sbjct: 249 VQNPSRVEVVSALIYKAIISASRNNNNSQNQSTLLLQTLNLRKRVVPPLPETLVGSLVSF 308

Query: 303 FPVAAGGEKEV-ELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNG 362
           FPV   GE+EV ELHELVG MR EM +FC++YAKKYRT+EW E IKKRL ES EIL+KNG
Sbjct: 309 FPVGVDGEREVVELHELVGTMRKEMGEFCDRYAKKYRTKEWLELIKKRLNESREILSKNG 368

Query: 363 GNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHN 422
            NQ VYR SSGC FPIYEVDFGWG A W+TM AF++KNTVMMLDAK+GGGIEAL+ L   
Sbjct: 369 NNQLVYRCSSGCNFPIYEVDFGWGVADWITMPAFKMKNTVMMLDAKNGGGIEALISLQDG 428

Query: 423 QMAAFLQNQELLSFAS 426
           +MAAF  N+ELL+FAS
Sbjct: 429 EMAAFQDNEELLAFAS 439

BLAST of MC08g0073 vs. ExPASy TrEMBL
Match: A0A6J1GI35 (acylsugar acyltransferase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111454400 PE=3 SV=1)

HSP 1 Score: 558 bits (1439), Expect = 1.37e-195
Identity = 288/435 (66.21%), Postives = 347/435 (79.77%), Query Frame = 0

Query: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60
           M L+IVSR++IKPSSPTPPHLK HALSLFDQLAPHM+VPLVFFFR  +  S +     S 
Sbjct: 16  MQLQIVSRQSIKPSSPTPPHLKTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCT-SH 75

Query: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSG-RLSEVVEKRRSEIVEV 120
            L+ SLS TLSRYYPFAGR+KDK  V+CNDEGVTFLEA +     L+E++E  +SEIVE+
Sbjct: 76  FLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEASLGGNVTLAEILEGPKSEIVEM 135

Query: 121 MFGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAA 180
           +F DGLQWK SK G LL +Q+T F+CGGFAVGVLLSHKL D+AT+V F+ DW  I+RD  
Sbjct: 136 LFVDGLQWKHSKMGPLLAIQVTYFQCGGFAVGVLLSHKLGDLATLVKFVTDWGFITRDTT 195

Query: 181 LGEQ-LVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVSDKVE 240
              Q  VNPLF +ADLFPLGDLP MSGAVIE+GK   C R VFE SKIEALK S+SDKV+
Sbjct: 196 NSAQHTVNPLFHSADLFPLGDLPPMSGAVIEEGKNLTCKRFVFEASKIEALKRSISDKVK 255

Query: 241 NPSRVEVVSALIYKTLISA----SPSPSN--SLMLQTLNLRTRVAPPLPENVVGSLVSFF 300
            P+RVEV++ALIYK +ISA    + +P+N  +L+LQT+NLRTR+ PPLPE+ +G++VSFF
Sbjct: 256 IPTRVEVMTALIYKAIISALRDSNSNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFF 315

Query: 301 PVAAG-GEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGG 360
           PVAA  GE+EVE+HE+VG MR EM +FC+KYAKK+RTEEW E IKKRL E  EIL+KNG 
Sbjct: 316 PVAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKFRTEEWVELIKKRLNEVREILSKNGK 375

Query: 361 NQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQ 420
            Q +YR SSGC FP+Y VDFGWGAAAWVTMAAF++KNTVMMLD K+GGGIEAL+ L   +
Sbjct: 376 PQLIYRCSSGCNFPVYGVDFGWGAAAWVTMAAFKMKNTVMMLDDKNGGGIEALISLDDKE 435

Query: 421 MAAFLQNQELLSFAS 426
           MAAF  N+ELL++ S
Sbjct: 436 MAAFQHNEELLAYTS 449

BLAST of MC08g0073 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.0e-73
Identity = 170/437 (38.90%), Postives = 257/437 (58.81%), Query Frame = 0

Query: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60
           M +++VSR+ IKPSSPTP HLK   LSL +QL P +F P+VFF+  +N    +  +   +
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQL---Q 60

Query: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM 120
            LK SLS+TL+ +YP AGR+K    ++CND G  FLEARV S  LS ++ +  S+ ++ +
Sbjct: 61  MLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNS-PLSNLLLEPSSDSLQQL 120

Query: 121 FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL 180
               +   E+++  LL+ Q + FECG  ++GV +SHKLAD  ++  F++ WA IS   ++
Sbjct: 121 IPTSVDSIETRT-RLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSI 180

Query: 181 GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGK----GFLCHRIVFEGSKIEALKNSVSD- 240
            + +  P+F    +FP G+    S A + + +      L  R +F+ S I+AL+   S  
Sbjct: 181 -KTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASSF 240

Query: 241 KVENPSRVEVVSALIYKTLISASPSPSN----SLMLQTLNLRTRVAPPLPENVVGSLVSF 300
           +V  P+RVE VSALI+K+ + A+ + S     S++  +++LR+RV+PP  +N +G+LVS+
Sbjct: 241 EVNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANSVSLRSRVSPPFTKNSIGNLVSY 300

Query: 301 FPV-AAGGEKEVELHELVGKMRDEMADFCNKYAKK-YRTEEWGEAIKKRLKESGEILTKN 360
           F   A  G  + +L  LV K+R     F + +  K        E I    KE+G+++   
Sbjct: 301 FAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDMIA-- 360

Query: 361 GGNQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGH 420
            G+ + Y FSS CRF +YE DFGWG   WV   + R KN V +LD K  GGIEA V L  
Sbjct: 361 SGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVNLNE 420

Query: 421 NQMAAFLQNQELLSFAS 427
            +M  F Q++ELL FAS
Sbjct: 421 QEMNLFEQDRELLQFAS 429

BLAST of MC08g0073 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 219.9 bits (559), Expect = 3.8e-57
Identity = 146/447 (32.66%), Postives = 243/447 (54.36%), Query Frame = 0

Query: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60
           M +E +++E IKPSS TP  L+   LS++D + P ++     F+  D+  S       S 
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQ---SSH 63

Query: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVE----KRRSEI 120
            LK SL++TL+++YP AGR+K    ++CNDEG  F++ARV +  LS+ +     K   ++
Sbjct: 64  KLKTSLAETLTKFYPLAGRIKG-VTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQL 123

Query: 121 VEVMFGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISR 180
           + +   D   ++ + +  LL+V+ T F CGG A+G+ ++HK+AD  ++  FIQ WA ++R
Sbjct: 124 LPLDVIDD-PYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMAR 183

Query: 181 DAALGEQLVNPLFVAADLFP----LGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNS 240
             A G+ + +P+F AA+ +P    L   P    A   +    +  R VF  SK+E L+  
Sbjct: 184 GEA-GDIVADPVFAAANFYPPANELFKFPVDENA---NKISQITKRFVFSASKLEELRTK 243

Query: 241 VS--DKVENPSRVEVVSALIYKTLISASPSPSNS----LMLQTLNLRTRVAPPLPENVVG 300
            +  D V  P RVE V+AL++K  ++A+ S +N+    +++Q  NLR ++   L E+++G
Sbjct: 244 AASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQPSNLRPKIPSLLAESLIG 303

Query: 301 SLV-SFFPVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEI 360
           +++ S   +    ++E+++ + V  +R +  D  N        EE G +      +   +
Sbjct: 304 NIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVI-----KEEGGSSSAMIGSKLANL 363

Query: 361 LTKNGG-----NQEVYRFSSGCRFPIYEVDFGWGAAAWVT-MAAFRLKNTVMMLDAKSGG 420
           +  N         E Y  SS C+ P+YE  FGWG+  W+    A  L+N  M++D+K G 
Sbjct: 364 MLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQ 423

Query: 421 GIEALVGLGHNQMAAFLQNQELLSFAS 427
           GIEA V L    M +  QN ELL+FAS
Sbjct: 424 GIEAFVTLPEENMLSLEQNTELLAFAS 436

BLAST of MC08g0073 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 219.2 bits (557), Expect = 6.5e-57
Identity = 145/434 (33.41%), Postives = 229/434 (52.76%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKI-HALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISES 62
           + + S+E +KPS     H    H LS  DQLAP +F+P +FF+ +  + S       S+ 
Sbjct: 5   ITVTSQELVKPSPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKE---RSDH 64

Query: 63  LKLSLSKTLSRYYPFAGRMKDK-ERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM 122
           +K SLS+ L+ YYP AGR+K+  + V CND GV+F+EA+     +S+++E      +  +
Sbjct: 65  IKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKA-DCNMSQILENPNPNELNKL 124

Query: 123 FGDGLQWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRDAAL 182
                ++ E  S   L VQ+T FECGG A+G+ LSHKL D  + + F+  WA  +R    
Sbjct: 125 --HPFEFHE-VSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQT- 184

Query: 183 GEQLVNPLFVAADLFPLGDLPAMSGAVIEDGKGFLCHRIVFEGSKIEALKNSVS-DKVEN 242
            ++++ P F  A +FP  D+  ++ A     +  +  R VF  S +E+L+   S +K   
Sbjct: 185 -DEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFSGNKKIR 244

Query: 243 PSRVEVVSALIYKTLISASPSPSNSLMLQTL----NLRTRVAPPLPENVVGSLVSF---F 302
            +RVEV+S  I+   ++++     +  + TL    NLR +  P +P+N+ G+++ F    
Sbjct: 245 ATRVEVLSVFIWSRFMASTNHDDKTGKIYTLIHPVNLRRQADPDIPDNMFGNIMRFSVTV 304

Query: 303 PVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTKNGGN 362
           P+    E + E   LV +MR+E+      Y KK + +  G       + SG +     G 
Sbjct: 305 PMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGFV----NGE 364

Query: 363 QEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVMMLDAKSGGGIEALVGLGHNQM 422
              + F+S C+FP+YE DFGWG   WV  A    KN V  +D K G GIEA + L  N M
Sbjct: 365 IVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQNDM 424

Query: 423 AAFLQNQELLSFAS 427
           + F  ++ELL + S
Sbjct: 425 SRFEADEELLRYVS 425

BLAST of MC08g0073 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 216.1 bits (549), Expect = 5.5e-56
Identity = 142/440 (32.27%), Postives = 244/440 (55.45%), Query Frame = 0

Query: 1   MLLEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISE 60
           M +E +S+E IKPSSPTP +L+   LS++D + P ++     F+  ++  S+      S 
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEH---TSH 65

Query: 61  SLKLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVM 120
            LK SLS+TL+++YP AGR+     V+C DEG  F++ARV +  L+E ++    + ++ +
Sbjct: 66  KLKTSLSETLTKFYPLAGRITG-VTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQL 125

Query: 121 FGDGL---QWKESKSGALLMVQITLFECGGFAVGVLLSHKLADMATMVNFIQDWALISRD 180
               +    +  + +  LL+V+ T F CGG A+G+ ++HK+AD A++  FI+ WA  +R 
Sbjct: 126 LPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARG 185

Query: 181 AALGEQLVNPLFVAADLFPLGDLPAMSGAVIEDGK-GFLCHRIVFEGSKIEALKNSVS-- 240
                 + +P+F  A+ +P  +      A  + GK   +  R VFE SK+E L+   +  
Sbjct: 186 ENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAASE 245

Query: 241 DKVENPSRVEVVSALIYKTLISASPSPS--NSLMLQTLNLRTRVAPPLPENVVGSLV-SF 300
           + V+ P+RVE V+ALI+K  +++S + +  + +++Q  NLR+++   L E+ +G+L+ S 
Sbjct: 246 ETVDQPTRVESVTALIWKCFVASSKTTTCDHKVLVQLANLRSKIPSLLQESSIGNLMFSS 305

Query: 301 FPVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKESGEILTK--- 360
             ++ G   EV++ E V  +R +  +       +  + +    I  +L  +  +LT    
Sbjct: 306 VVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKL--ANLMLTNYSR 365

Query: 361 -NGGNQEVYRFSSGCRFPIYEVDFGWGAAAWVT-MAAFRLKNTVMMLDAKSGGGIEALVG 420
            +    E Y  SS C+ P+YE  FGW +  WV    +  L N  M++D+K G GIEA V 
Sbjct: 366 LSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDGQGIEAFVT 425

Query: 421 LGHNQMAAFLQNQELLSFAS 427
           L    M++F QN ELL+FA+
Sbjct: 426 LPEENMSSFEQNPELLAFAT 439

BLAST of MC08g0073 vs. TAIR 10
Match: AT5G47980.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.6e-53
Identity = 148/438 (33.79%), Postives = 233/438 (53.20%), Query Frame = 0

Query: 3   LEIVSRETIKPSSPTPPHLKIHALSLFDQLAPHMFVPLVFFFRHDNDCSRSSIVVISESL 62
           LE++ RE IKPSSP  PH ++  LS+ D       VP++FF+ +  D +  S  ++S  L
Sbjct: 5   LEVIQREVIKPSSPA-PHDRLQ-LSVIDFGIAEACVPMIFFY-NLADLAEKSPDIVSTRL 64

Query: 63  KLSLSKTLSRYYPFAGRMKDKERVECNDEGVTFLEARVRSGRLSEVVEKRRSEIVEVMFG 122
           + SLS+ LSR+YP AG+ K+   + CNDEG  F EAR     LSE +       ++++  
Sbjct: 65  RSSLSQALSRFYPLAGK-KEGVSISCNDEGAVFTEARTNL-LLSEFLRNIDINSLKILIP 124

Query: 123 DGLQWKESKSGALLMVQITLFECG-GFAVGVLLSHKLADMATMVNFIQDWALISRDAALG 182
                +   S  LL VQ T F  G G AVG+ +SH + D A++  F++ WA  +R  +  
Sbjct: 125 TLAPGESLDSRPLLSVQATFFGSGSGLAVGICVSHCICDAASVSTFVRGWAATARGDS-N 184

Query: 183 EQLVNPLFVAADLFPLGDLP---AMSGAVIEDGKGFLCHRIVFEGSKIEALK-NSVSDKV 242
           ++L  P F    + P  D+    +   A+ E  +  + +R VFE  KI  LK  + S  V
Sbjct: 185 DELSTPQFAEVAIHPPADISIHGSPFNALSEVREKCVTNRFVFESDKITKLKIVAASKSV 244

Query: 243 ENPSRVEVVSALIYKTLISASPS----PSNSLMLQTLNLRTRV-APPLPENVVGSLVSFF 302
            +P+RVE V +LI++   +AS +    P  ++M Q+++LR R+    L  + +G+L   F
Sbjct: 245 PSPTRVEAVMSLIWRCARNASHANLIVPRATMMTQSMDLRLRIPTNVLSPDAIGNLQGVF 304

Query: 303 PVAAGGEKEVELHELVGKMRDEMADFCNKYAKKYRTEEWGEAIKKRLKES-GEILTKNGG 362
            +  G   E+E+ E+V + R E  +F     +          + +++       +++   
Sbjct: 305 FLKRGPGSEIEISEVVAEFRKEKEEFNEMIKENVNGGHTNTTLGQKIMSGIANYMSELKP 364

Query: 363 NQEVYRFSSGCRFPIYEVDFGWGAAAWVTMAAFRLKNTVM---MLDAKSGGGIEALVGLG 422
           N + Y  SS CR   YEVDFGWG  AWV +    +++ VM   ++DAK G G+E  VG+ 
Sbjct: 365 NIDTYTMSSWCRKAFYEVDFGWGRPAWVGLGHQDIQDGVMYVLLVDAKDGEGVEVWVGIP 424

Query: 423 HNQMAAFLQNQELLSFAS 427
              MAAF+ +QELLS+AS
Sbjct: 425 EQDMAAFVCDQELLSYAS 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
D2Y3X21.7e-6534.63Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1[more]
Q58VT11.7e-6534.86Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1[more]
A0A2G3D6U12.2e-6534.63Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1[more]
A0A2K8FQU59.2e-5633.49Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
A0A2P1GIW72.8e-5232.31Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022132095.19.11e-306100.00BAHD acyltransferase At5g47980-like [Momordica charantia][more]
XP_038886275.17.51e-20870.86stemmadenine O-acetyltransferase-like [Benincasa hispida][more]
XP_008444410.14.31e-19967.89PREDICTED: vinorine synthase [Cucumis melo][more]
XP_023538329.17.35e-19967.20acylsugar acyltransferase 3-like [Cucurbita pepo subsp. pepo][more]
XP_004143047.11.69e-19867.74stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62352.1 hypothetical prot... [more]
Match NameE-valueIdentityDescription
A0A6J1BRH34.41e-306100.00BAHD acyltransferase At5g47980-like OS=Momordica charantia OX=3673 GN=LOC1110050... [more]
A0A1S3BB282.09e-19967.89vinorine synthase OS=Cucumis melo OX=3656 GN=LOC103487747 PE=3 SV=1[more]
A0A0A0LNF88.16e-19967.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350350 PE=3 SV=1[more]
A0A5A7V5H25.87e-19667.20Vinorine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00... [more]
A0A6J1GI351.37e-19566.21acylsugar acyltransferase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111454400 P... [more]
Match NameE-valueIdentityDescription
AT3G26040.11.0e-7338.90HXXXD-type acyl-transferase family protein [more]
AT3G30280.13.8e-5732.66HXXXD-type acyl-transferase family protein [more]
AT1G24430.16.5e-5733.41HXXXD-type acyl-transferase family protein [more]
AT4G15390.15.5e-5632.27HXXXD-type acyl-transferase family protein [more]
AT5G47980.12.6e-5333.79HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..199
e-value: 4.6E-55
score: 188.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 213..423
e-value: 4.0E-48
score: 165.4
IPR003480TransferasePFAMPF02458Transferasecoord: 3..421
e-value: 1.2E-73
score: 248.2
NoneNo IPR availablePANTHERPTHR31623:SF51N-BENZOYLTRANSFERASE, PUTATIVE-RELATEDcoord: 3..424
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 3..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0073.1MC08g0073.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups