MC08g0034 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0034
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic
LocationMC08: 230793 .. 232624 (+)
RNA-Seq ExpressionMC08g0034
SyntenyMC08g0034
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTCCCTCCAACTCCAACTCCGTCTGCCTTCTTCTACCACTCCAACTCTTCAAACTCACCGTCCGATTCGATGCGCCGCCGATCGCCAGGCATTGTTCAACCGTATTGCGCCTGTTTATGATAACGTCCGCTTAATTTCATCTCTCTCTTCCTCCCTTAATACTTCCCTATTTCCCTTTCCCTTCTCCAACTTCTTACCTTTCTTTCTGCTTCCAATTTAGTTGAACGACTTACTCAGTTTGGGTCAGCATCGCATTTGGAAACGAATGGCCGTGTCCTGGAGCGGGTATGTAACGTATCCTTCTTTTCTGTTCCCGGATTGCCTTATCTTATTTATTTTTTAACTTTCCCACATTGCGAACAGTGCTAAACCTGGAGATCGTGTGCTGGATATATGCTGTGGAAGCGGCGATTTGGCGTTCCTCTTATCTGAGAGACTTGGATCCGATGGCAAGGTACCCCCACCGGTTTGTTTTTGTACCAATACTGAAGATAATTGTATCTCATCATTCAATTCTTTGGCACAGGTGACAGGTCTTGATTTCTCAAGGGAGCAGCTGATTATTGCATCCTCTCGTCAACGTTCGCTCTCCAAAGCCTGCTATAAGAACATTGAGTGAGCATTGGTAAACTTGAACAGCAAATATGTTAGCCTGTTCAATTTGGGAAATTCTTTAGTATTCCACTCCATATTTATCTCATACATATCAAACTATGACGATATAGTATTTCTGTAGTAAGAATTACGATTTCTGCTATTTGTAATTGCTTGTTGAATCATCGTCCTAATACTGCTGCTGCCAAAATGAAGGTGGGTGGAAGGTGATGCCCTAAATTTGCCATTTTCTGACGGTTACTTTGATGCCATTACAATGGGCTATGGTCTACGGAATGTGGTGGATAAACGGAAAGCTCTGGAGGAAATGCTTAGAGTACTTAAAGAAGGTCTTTTGTTTTGTTTTGTTTTTTTTGTTTTTTCTCTATTTTGAGAAACGAGAATTGTGCCATTTCACAGGAGAAACACGGTCCTTTTTCTTTCTTTCTTTCTTTCTCTCTCTCTGTTTTTTTTTTTTTTTGGGGAGATAAACCCACTTATGACGTGGAAATCACCACTTTTNTCAGTCCTCTGATTTTCTCTACGTTAATTAGCGCTGGAAGGGACAATGAATATAAGCACTTAAGGCTGAACCGCTGACTCCCAAGAATGATCTGTCTCTTTACTCTTTCTTGATTGATGACATGGTAAAATTCAGTTACAAAATTTGCATACGTATTCTTGATGAAAAAAGTGGTCTTTCCAGGTAGTAAGGTATCAATTCTCGACTTTAACAAGACAACACAACCAACTGCTGCTGCTATTCAGGTACTTTTATGTAAAGATTTGTCTGAAGGCAAAAGTTTTCTCTTGTGTGGCACACATTTCAAATTTAGTGAGTTTCTACTTCTGTTTGTATGTCCATTCTTCTGGACTTCCTCTATGCAAGGAATGGATGATAAACAACATTGTTGTCCCAGTGGCATCTGGTTATGGCCTTGCAGAGGATTATAAATATCTGTCCAAATCCATCAAAGAATTTTTTACAGGTACTCTATCTCTCTTGACAGGGCTGCTGCTGCTATTAGTTATTCTGTAAACAAAAGCCTCTTGCATTTATTTTTTAGTCTATAATTATGGATTTGGAATCATCCTTTAGTATCACCCAGTGACTAAATTTGTTTTTATACAGGAAAGGAATTGGAAGAACTTGCTTTAGAAGTAGGGTTTTCTACATCCATACATTATGAAATTAGTGGAGGTCTCATGGGAAATTTGGTGGCATCTCGT

mRNA sequence

ATGGCTTCCTCCCTCCAACTCCAACTCCGTCTGCCTTCTTCTACCACTCCAACTCTTCAAACTCACCGTCCGATTCGATGCGCCGCCGATCGCCAGGCATTGTTCAACCGTATTGCGCCTGTTTATGATAACTTGAACGACTTACTCAGTTTGGGTCAGCATCGCATTTGGAAACGAATGGCCGTGTCCTGGAGCGGTGCTAAACCTGGAGATCGTGTGCTGGATATATGCTGTGGAAGCGGCGATTTGGCGTTCCTCTTATCTGAGAGACTTGGATCCGATGGCAAGGTGACAGGTCTTGATTTCTCAAGGGAGCAGCTGATTATTGCATCCTCTCGTCAACGTTCGCTCTCCAAAGCCTGCTATAAGAACATTGAGTGGGTGGAAGGTGATGCCCTAAATTTGCCATTTTCTGACGGTTACTTTGATGCCATTACAATGGGCTATGGTCTACGGAATGTGGTGGATAAACGGAAAGCTCTGGAGGAAATGCTTAGAGTACTTAAAGAAGGTAGTAAGGTATCAATTCTCGACTTTAACAAGACAACACAACCAACTGCTGCTGCTATTCAGGAATGGATGATAAACAACATTGTTGTCCCAGTGGCATCTGGTTATGGCCTTGCAGAGGATTATAAATATCTGTCCAAATCCATCAAAGAATTTTTTACAGGAAAGGAATTGGAAGAACTTGCTTTAGAAGTAGGGTTTTCTACATCCATACATTATGAAATTAGTGGAGGTCTCATGGGAAATTTGGTGGCATCTCGT

Coding sequence (CDS)

ATGGCTTCCTCCCTCCAACTCCAACTCCGTCTGCCTTCTTCTACCACTCCAACTCTTCAAACTCACCGTCCGATTCGATGCGCCGCCGATCGCCAGGCATTGTTCAACCGTATTGCGCCTGTTTATGATAACTTGAACGACTTACTCAGTTTGGGTCAGCATCGCATTTGGAAACGAATGGCCGTGTCCTGGAGCGGTGCTAAACCTGGAGATCGTGTGCTGGATATATGCTGTGGAAGCGGCGATTTGGCGTTCCTCTTATCTGAGAGACTTGGATCCGATGGCAAGGTGACAGGTCTTGATTTCTCAAGGGAGCAGCTGATTATTGCATCCTCTCGTCAACGTTCGCTCTCCAAAGCCTGCTATAAGAACATTGAGTGGGTGGAAGGTGATGCCCTAAATTTGCCATTTTCTGACGGTTACTTTGATGCCATTACAATGGGCTATGGTCTACGGAATGTGGTGGATAAACGGAAAGCTCTGGAGGAAATGCTTAGAGTACTTAAAGAAGGTAGTAAGGTATCAATTCTCGACTTTAACAAGACAACACAACCAACTGCTGCTGCTATTCAGGAATGGATGATAAACAACATTGTTGTCCCAGTGGCATCTGGTTATGGCCTTGCAGAGGATTATAAATATCTGTCCAAATCCATCAAAGAATTTTTTACAGGAAAGGAATTGGAAGAACTTGCTTTAGAAGTAGGGTTTTCTACATCCATACATTATGAAATTAGTGGAGGTCTCATGGGAAATTTGGTGGCATCTCGT

Protein sequence

MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR
Homology
BLAST of MC08g0034 vs. ExPASy Swiss-Prot
Match: Q3ED65 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MENG PE=1 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 9.7e-93
Identity = 160/233 (68.67%), Postives = 195/233 (83.69%), Query Frame = 0

Query: 25  IRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLA 84
           ++C+ +R+ LFNRIAPVYDNLNDLLSLGQHRIWK MAVSWSGAK GD VLD+CCGSGDLA
Sbjct: 29  VKCSNERRILFNRIAPVYDNLNDLLSLGQHRIWKNMAVSWSGAKKGDYVLDLCCGSGDLA 88

Query: 85  FLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDA 144
           FLLSE++GS GKV GLDFS EQL +A++RQ   +++CYK IEW+EGDA++LPF D  FDA
Sbjct: 89  FLLSEKVGSTGKVMGLDFSSEQLAVAATRQSLKARSCYKCIEWIEGDAIDLPFDDCEFDA 148

Query: 145 ITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVAS 204
           +TMGYGLRNVVD+ +A++EM RVLK GS+VSILDFNK+ Q     +Q WMI+N+VVPVA+
Sbjct: 149 VTMGYGLRNVVDRLRAMKEMYRVLKPGSRVSILDFNKSNQSVTTFMQGWMIDNVVVPVAT 208

Query: 205 GYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 258
            Y LA++Y+YL  SI  + TG+ELE LALE GFS++ HYEISGG MGNLVA R
Sbjct: 209 VYDLAKEYEYLKYSINGYLTGEELETLALEAGFSSACHYEISGGFMGNLVAMR 261

BLAST of MC08g0034 vs. ExPASy Swiss-Prot
Match: Q8YLP4 (2-phytyl-1,4-naphtoquinone methyltransferase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=menG PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 5.3e-67
Identity = 119/226 (52.65%), Postives = 165/226 (73.01%), Query Frame = 0

Query: 32  QALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLAFLLSERL 91
           +A+F+RIAPVYD LND LSLGQHRIWK MA+ W+GAKPGD  LD+CCGSGDLA  L+ R+
Sbjct: 6   RAIFDRIAPVYDQLNDWLSLGQHRIWKEMAIKWTGAKPGDTCLDLCCGSGDLALRLARRV 65

Query: 92  GSDGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDAITMGYGL 151
           GS G+V+G+DFS    ++ +++QR+ S+    NI WVE + L+LPF D  FDA TMGYGL
Sbjct: 66  GSTGQVSGVDFSAN--LLETAKQRAQSQYPQPNISWVEANVLDLPFKDNQFDAATMGYGL 125

Query: 152 RNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAED 211
           RNV D  ++L+E+ RVLK  +K +ILDF++         Q+W +++IVVP+A   G+ E+
Sbjct: 126 RNVTDIPRSLQELHRVLKPNAKAAILDFHRPNNQQFRTFQQWYLDSIVVPLADRLGVKEE 185

Query: 212 YKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 258
           Y Y+S S+  F  GKE  E+AL+VGF+++ HY I+ G+MG L+ S+
Sbjct: 186 YAYISPSLDRFPIGKEQVEIALKVGFTSATHYPIANGMMGVLIISK 229

BLAST of MC08g0034 vs. ExPASy Swiss-Prot
Match: Q3MD91 (2-phytyl-1,4-naphtoquinone methyltransferase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=menG PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 5.9e-66
Identity = 117/226 (51.77%), Postives = 164/226 (72.57%), Query Frame = 0

Query: 32  QALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLAFLLSERL 91
           +A+F+RIAPVYD LND LSLGQHRIWK MA+ W+GAKPGD  LD+CCGSGDLA  L+ R+
Sbjct: 6   RAIFDRIAPVYDQLNDWLSLGQHRIWKEMAIKWTGAKPGDTCLDLCCGSGDLALRLARRV 65

Query: 92  GSDGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDAITMGYGL 151
           GS G+V G+DFS    ++ +++QR+ ++    +I WVE + L+LPF D  FDA TMGYGL
Sbjct: 66  GSTGQVYGVDFSAN--LLETAKQRAQAQYPQPHISWVEANVLDLPFEDNQFDAATMGYGL 125

Query: 152 RNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAED 211
           RNV D  ++L+E+ RVLK  +K +ILDF++         Q+W +++IVVP+A   G+ E+
Sbjct: 126 RNVTDIPRSLQELRRVLKPNAKAAILDFHRPNNQQFRTFQQWYLDSIVVPLADRLGVKEE 185

Query: 212 YKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 258
           Y Y+S S+  F  GKE  E+AL+VGF+++ HY I+ G+MG L+ S+
Sbjct: 186 YAYISPSLDRFPIGKEQVEIALQVGFTSATHYPIANGMMGVLIISK 229

BLAST of MC08g0034 vs. ExPASy Swiss-Prot
Match: B2IUM7 (2-phytyl-1,4-naphtoquinone methyltransferase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=menG PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.7e-65
Identity = 123/226 (54.42%), Postives = 160/226 (70.80%), Query Frame = 0

Query: 32  QALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLAFLLSERL 91
           Q++FNRIAPVYD LND LSLGQHRIWK MAV WS AK G+  LD+CCGSGDLA  L+ R+
Sbjct: 6   QSIFNRIAPVYDQLNDWLSLGQHRIWKEMAVKWSAAKSGNTALDLCCGSGDLALRLARRV 65

Query: 92  GSDGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDAITMGYGL 151
           G+ G V G+DFS    ++ ++++RS  +     I WVE D LNLPF D  FDA TMGYGL
Sbjct: 66  GATGYVYGVDFSCN--LLETAKERSQKQYPQPAIAWVEADVLNLPFDDNQFDAATMGYGL 125

Query: 152 RNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAED 211
           RNV D  ++L+E+ RVLK G+K +ILDF++ + P   A Q+  +N+ VVPVA+  GL E+
Sbjct: 126 RNVKDIPRSLQELHRVLKPGAKAAILDFHRPSNPQLRAFQQLYLNSFVVPVANYLGLKEE 185

Query: 212 YKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 258
           Y Y+S S+  F  GKE  ELA +VGF+ + HY I+ G+MG LV S+
Sbjct: 186 YAYISPSLDRFPIGKEQIELARQVGFAVATHYPIANGMMGVLVVSK 229

BLAST of MC08g0034 vs. ExPASy Swiss-Prot
Match: P72818 (2-phytyl-1,4-naphtoquinone methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=menG PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.1e-64
Identity = 122/226 (53.98%), Postives = 157/226 (69.47%), Query Frame = 0

Query: 32  QALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLAFLLSERL 91
           Q +F RIAP YD+LN  LS GQH IWK MAV WSG  PGDR+LD+CCGSGDLAF  ++ +
Sbjct: 15  QQIFARIAPQYDDLNTFLSFGQHHIWKAMAVKWSGVSPGDRLLDVCCGSGDLAFQGAKVV 74

Query: 92  GSDGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDAITMGYGL 151
           G+ GKV GLDF  E L IA+ + +  SK  +  ++W++GDAL LPFSD  FD  TMGYGL
Sbjct: 75  GTRGKVVGLDFCAELLAIAAGKHK--SKYAHLPMQWLQGDALALPFSDNEFDGATMGYGL 134

Query: 152 RNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAED 211
           RNV +  +AL E+ RVLK G KV+ILDF++     AA  Q W + N+VVP+A  + L E+
Sbjct: 135 RNVGNIPQALTELQRVLKPGKKVAILDFHQPGNALAANFQRWYLANVVVPMAKQWRLTEE 194

Query: 212 YKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 258
           Y YL  S+  F TG +  + ALEVGF+ ++HY I+ GLMG LVA +
Sbjct: 195 YAYLQPSLDRFPTGPKQVQFALEVGFAKAVHYPIAAGLMGVLVAEK 238

BLAST of MC08g0034 vs. NCBI nr
Match: XP_022131612.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 511 bits (1316), Expect = 1.27e-182
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA
Sbjct: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
           CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN
Sbjct: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240
           KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS
Sbjct: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240

Query: 241 IHYEISGGLMGNLVASR 257
           IHYEISGGLMGNLVASR
Sbjct: 241 IHYEISGGLMGNLVASR 257

BLAST of MC08g0034 vs. NCBI nr
Match: XP_022951506.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 457 bits (1176), Expect = 5.75e-161
Identity = 232/257 (90.27%), Postives = 236/257 (91.83%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSL LQLRLP  T P    HRPI+CAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 21  MASSLPLQLRLPPPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 80

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAK GD VLDICCGSGDLAFLLSER+G DGKVTGLDFS+EQL IASSRQ SLSK 
Sbjct: 81  AVSWSGAKAGDCVLDICCGSGDLAFLLSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKP 140

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
           CY NIEWVEGDALNLPFSDG FDAITMGYGLRNVVDKRKALEEMLRVLK+GSKVSILDFN
Sbjct: 141 CYNNIEWVEGDALNLPFSDGTFDAITMGYGLRNVVDKRKALEEMLRVLKQGSKVSILDFN 200

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240
           KTTQP  AAIQEWMINNIVVPVASGYGLAEDYKYLSKSI EF TGKELEELALEVGFSTS
Sbjct: 201 KTTQPITAAIQEWMINNIVVPVASGYGLAEDYKYLSKSINEFSTGKELEELALEVGFSTS 260

Query: 241 IHYEISGGLMGNLVASR 257
            HYEISGGLMGNLVASR
Sbjct: 261 RHYEISGGLMGNLVASR 277

BLAST of MC08g0034 vs. NCBI nr
Match: XP_023545823.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 456 bits (1172), Expect = 1.13e-160
Identity = 228/257 (88.72%), Postives = 240/257 (93.39%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSLQLQ RLP STTP L  HRPI+CA DRQALF+RIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQFRLPPSTTPALLRHRPIQCATDRQALFDRIAPVYDNLNDLLSLGQHRIWKRM 60

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAK GD VLDICCGSGDLAFLLS+++GS+GKVTGLDFSR+QL IASSRQ SLS+ 
Sbjct: 61  AVSWSGAKAGDCVLDICCGSGDLAFLLSQKVGSNGKVTGLDFSRQQLSIASSRQTSLSRP 120

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
           CYKNIEWVEGDALNLPFSDG  DAITMGYGLRNV+DKRKALEEMLRVLKEGSK+SILDFN
Sbjct: 121 CYKNIEWVEGDALNLPFSDGSVDAITMGYGLRNVLDKRKALEEMLRVLKEGSKLSILDFN 180

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240
           K TQPT AAIQEWMINNIVVP+ASGYGLAEDYKYLSKSI EF TGKELE+LALEVGFSTS
Sbjct: 181 KATQPTTAAIQEWMINNIVVPMASGYGLAEDYKYLSKSISEFSTGKELEKLALEVGFSTS 240

Query: 241 IHYEISGGLMGNLVASR 257
           IHYEISGGLMGNLVASR
Sbjct: 241 IHYEISGGLMGNLVASR 257

BLAST of MC08g0034 vs. NCBI nr
Match: XP_038885206.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 454 bits (1169), Expect = 3.25e-160
Identity = 231/257 (89.88%), Postives = 238/257 (92.61%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSLQLQLRLP STTP L  HRPI+CA+DRQALFNRIAPVYD LNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQLRLPPSTTPPLLRHRPIQCASDRQALFNRIAPVYDTLNDLLSLGQHRIWKRM 60

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAK GD VLD+CCGSGDLAFLLS+++GS GKVTGLDFSREQL IASSRQ SLSK 
Sbjct: 61  AVSWSGAKAGDCVLDLCCGSGDLAFLLSQKIGSVGKVTGLDFSREQLSIASSRQNSLSKP 120

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
            Y NIEWVEGDALNLPF D  FDAITMGYGLRNV+DKRKALEEMLRVLKEGSKVSILDFN
Sbjct: 121 FYNNIEWVEGDALNLPFPDCSFDAITMGYGLRNVMDKRKALEEMLRVLKEGSKVSILDFN 180

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240
           KTTQPT AAIQEWMINNIVVPVASGYGLAEDYKYLSKSI EF TGKELEELALEVGFSTS
Sbjct: 181 KTTQPTTAAIQEWMINNIVVPVASGYGLAEDYKYLSKSINEFSTGKELEELALEVGFSTS 240

Query: 241 IHYEISGGLMGNLVASR 257
           IHYEISGGLMGNLVASR
Sbjct: 241 IHYEISGGLMGNLVASR 257

BLAST of MC08g0034 vs. NCBI nr
Match: XP_023002763.1 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 455 bits (1171), Expect = 3.32e-160
Identity = 230/257 (89.49%), Postives = 236/257 (91.83%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSL LQLRLP  T P    HRPI+CAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 21  MASSLPLQLRLPLPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 80

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAK GD VLDICCGSGDLAFL+SER+G DGKVTGLDFS+EQL IASSRQ SLSK 
Sbjct: 81  AVSWSGAKAGDCVLDICCGSGDLAFLVSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKP 140

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
           CY NIEWVEGDALNLPFSDG FDAITMGYGLRNVVDKRKALEEMLRVLK+GSK+SILDFN
Sbjct: 141 CYNNIEWVEGDALNLPFSDGTFDAITMGYGLRNVVDKRKALEEMLRVLKQGSKISILDFN 200

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240
           KTTQP  AAIQEWMINNIVVPVASGYGLAEDYKYLSKSI EF TGKELEELALEVGFSTS
Sbjct: 201 KTTQPITAAIQEWMINNIVVPVASGYGLAEDYKYLSKSINEFSTGKELEELALEVGFSTS 260

Query: 241 IHYEISGGLMGNLVASR 257
            HYEISGGLMGNLVASR
Sbjct: 261 RHYEISGGLMGNLVASR 277

BLAST of MC08g0034 vs. ExPASy TrEMBL
Match: A0A6J1BRH7 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111004744 PE=3 SV=1)

HSP 1 Score: 511 bits (1316), Expect = 6.16e-183
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA
Sbjct: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
           CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN
Sbjct: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240
           KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS
Sbjct: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240

Query: 241 IHYEISGGLMGNLVASR 257
           IHYEISGGLMGNLVASR
Sbjct: 241 IHYEISGGLMGNLVASR 257

BLAST of MC08g0034 vs. ExPASy TrEMBL
Match: A0A6J1GHS2 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111454302 PE=3 SV=1)

HSP 1 Score: 457 bits (1176), Expect = 2.78e-161
Identity = 232/257 (90.27%), Postives = 236/257 (91.83%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSL LQLRLP  T P    HRPI+CAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 21  MASSLPLQLRLPPPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 80

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAK GD VLDICCGSGDLAFLLSER+G DGKVTGLDFS+EQL IASSRQ SLSK 
Sbjct: 81  AVSWSGAKAGDCVLDICCGSGDLAFLLSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKP 140

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
           CY NIEWVEGDALNLPFSDG FDAITMGYGLRNVVDKRKALEEMLRVLK+GSKVSILDFN
Sbjct: 141 CYNNIEWVEGDALNLPFSDGTFDAITMGYGLRNVVDKRKALEEMLRVLKQGSKVSILDFN 200

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240
           KTTQP  AAIQEWMINNIVVPVASGYGLAEDYKYLSKSI EF TGKELEELALEVGFSTS
Sbjct: 201 KTTQPITAAIQEWMINNIVVPVASGYGLAEDYKYLSKSINEFSTGKELEELALEVGFSTS 260

Query: 241 IHYEISGGLMGNLVASR 257
            HYEISGGLMGNLVASR
Sbjct: 261 RHYEISGGLMGNLVASR 277

BLAST of MC08g0034 vs. ExPASy TrEMBL
Match: A0A6J1KUI9 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496525 PE=3 SV=1)

HSP 1 Score: 455 bits (1171), Expect = 1.61e-160
Identity = 230/257 (89.49%), Postives = 236/257 (91.83%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSL LQLRLP  T P    HRPI+CAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 21  MASSLPLQLRLPLPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 80

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAK GD VLDICCGSGDLAFL+SER+G DGKVTGLDFS+EQL IASSRQ SLSK 
Sbjct: 81  AVSWSGAKAGDCVLDICCGSGDLAFLVSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKP 140

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
           CY NIEWVEGDALNLPFSDG FDAITMGYGLRNVVDKRKALEEMLRVLK+GSK+SILDFN
Sbjct: 141 CYNNIEWVEGDALNLPFSDGTFDAITMGYGLRNVVDKRKALEEMLRVLKQGSKISILDFN 200

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTS 240
           KTTQP  AAIQEWMINNIVVPVASGYGLAEDYKYLSKSI EF TGKELEELALEVGFSTS
Sbjct: 201 KTTQPITAAIQEWMINNIVVPVASGYGLAEDYKYLSKSINEFSTGKELEELALEVGFSTS 260

Query: 241 IHYEISGGLMGNLVASR 257
            HYEISGGLMGNLVASR
Sbjct: 261 RHYEISGGLMGNLVASR 277

BLAST of MC08g0034 vs. ExPASy TrEMBL
Match: A0A6J1GIZ3 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111454302 PE=3 SV=1)

HSP 1 Score: 451 bits (1161), Expect = 6.20e-159
Identity = 232/261 (88.89%), Postives = 236/261 (90.42%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSL LQLRLP  T P    HRPI+CAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 21  MASSLPLQLRLPPPTNPPFLRHRPIQCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 80

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAK GD VLDICCGSGDLAFLLSER+G DGKVTGLDFS+EQL IASSRQ SLSK 
Sbjct: 81  AVSWSGAKAGDCVLDICCGSGDLAFLLSERVGYDGKVTGLDFSQEQLSIASSRQNSLSKP 140

Query: 121 CYKNIE----WVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSI 180
           CY NIE    WVEGDALNLPFSDG FDAITMGYGLRNVVDKRKALEEMLRVLK+GSKVSI
Sbjct: 141 CYNNIEVLLQWVEGDALNLPFSDGTFDAITMGYGLRNVVDKRKALEEMLRVLKQGSKVSI 200

Query: 181 LDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVG 240
           LDFNKTTQP  AAIQEWMINNIVVPVASGYGLAEDYKYLSKSI EF TGKELEELALEVG
Sbjct: 201 LDFNKTTQPITAAIQEWMINNIVVPVASGYGLAEDYKYLSKSINEFSTGKELEELALEVG 260

Query: 241 FSTSIHYEISGGLMGNLVASR 257
           FSTS HYEISGGLMGNLVASR
Sbjct: 261 FSTSRHYEISGGLMGNLVASR 281

BLAST of MC08g0034 vs. ExPASy TrEMBL
Match: A0A6J1BQR3 (2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111004744 PE=3 SV=1)

HSP 1 Score: 448 bits (1153), Expect = 1.81e-158
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0

Query: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60
           MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM
Sbjct: 1   MASSLQLQLRLPSSTTPTLQTHRPIRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRM 60

Query: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120
           AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA
Sbjct: 61  AVSWSGAKPGDRVLDICCGSGDLAFLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKA 120

Query: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180
           CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN
Sbjct: 121 CYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFN 180

Query: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFT 224
           KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFT
Sbjct: 181 KTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKSIKEFFT 224

BLAST of MC08g0034 vs. TAIR 10
Match: AT1G23360.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 6.9e-94
Identity = 160/233 (68.67%), Postives = 195/233 (83.69%), Query Frame = 0

Query: 25  IRCAADRQALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLA 84
           ++C+ +R+ LFNRIAPVYDNLNDLLSLGQHRIWK MAVSWSGAK GD VLD+CCGSGDLA
Sbjct: 29  VKCSNERRILFNRIAPVYDNLNDLLSLGQHRIWKNMAVSWSGAKKGDYVLDLCCGSGDLA 88

Query: 85  FLLSERLGSDGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDA 144
           FLLSE++GS GKV GLDFS EQL +A++RQ   +++CYK IEW+EGDA++LPF D  FDA
Sbjct: 89  FLLSEKVGSTGKVMGLDFSSEQLAVAATRQSLKARSCYKCIEWIEGDAIDLPFDDCEFDA 148

Query: 145 ITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVAS 204
           +TMGYGLRNVVD+ +A++EM RVLK GS+VSILDFNK+ Q     +Q WMI+N+VVPVA+
Sbjct: 149 VTMGYGLRNVVDRLRAMKEMYRVLKPGSRVSILDFNKSNQSVTTFMQGWMIDNVVVPVAT 208

Query: 205 GYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 258
            Y LA++Y+YL  SI  + TG+ELE LALE GFS++ HYEISGG MGNLVA R
Sbjct: 209 VYDLAKEYEYLKYSINGYLTGEELETLALEAGFSSACHYEISGGFMGNLVAMR 261

BLAST of MC08g0034 vs. TAIR 10
Match: AT1G23360.3 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 212.2 bits (539), Expect = 4.8e-55
Identity = 101/159 (63.52%), Postives = 128/159 (80.50%), Query Frame = 0

Query: 99  GLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKR 158
           GLDFS EQL +A++RQ   +++CYK IEW+EGDA++LPF D  FDA+TMGYGLRNVVD+ 
Sbjct: 2   GLDFSSEQLAVAATRQSLKARSCYKCIEWIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRL 61

Query: 159 KALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKS 218
           +A++EM RVLK GS+VSILDFNK+ Q     +Q WMI+N+VVPVA+ Y LA++Y+YL  S
Sbjct: 62  RAMKEMYRVLKPGSRVSILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYS 121

Query: 219 IKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 258
           I  + TG+ELE LALE GFS++ HYEISGG MGNLVA R
Sbjct: 122 INGYLTGEELETLALEAGFSSACHYEISGGFMGNLVAMR 160

BLAST of MC08g0034 vs. TAIR 10
Match: AT1G23360.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 212.2 bits (539), Expect = 4.8e-55
Identity = 101/159 (63.52%), Postives = 128/159 (80.50%), Query Frame = 0

Query: 99  GLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFSDGYFDAITMGYGLRNVVDKR 158
           GLDFS EQL +A++RQ   +++CYK IEW+EGDA++LPF D  FDA+TMGYGLRNVVD+ 
Sbjct: 2   GLDFSSEQLAVAATRQSLKARSCYKCIEWIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRL 61

Query: 159 KALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQEWMINNIVVPVASGYGLAEDYKYLSKS 218
           +A++EM RVLK GS+VSILDFNK+ Q     +Q WMI+N+VVPVA+ Y LA++Y+YL  S
Sbjct: 62  RAMKEMYRVLKPGSRVSILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYS 121

Query: 219 IKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLVASR 258
           I  + TG+ELE LALE GFS++ HYEISGG MGNLVA R
Sbjct: 122 INGYLTGEELETLALEAGFSSACHYEISGGFMGNLVAMR 160

BLAST of MC08g0034 vs. TAIR 10
Match: AT5G57300.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 3.2e-22
Identity = 70/241 (29.05%), Postives = 111/241 (46.06%), Query Frame = 0

Query: 34  LFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLAFLLSERLGS 93
           +F  +A  YD +ND++S G HR+WK   V       G + LD+  G+GD+AF + + + S
Sbjct: 48  VFTNVASSYDIMNDVMSGGLHRLWKERLVGKLSPFAGMKHLDVAGGTGDVAFRIYDAVYS 107

Query: 94  ---------------DGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFS 153
                          + ++   D +   L +   R         K++ WVEGDA  L F 
Sbjct: 108 VKRRALQKVDEASLEETQIYVCDINPNMLNVGKQRAAERGLRDNKSLVWVEGDAEALSFD 167

Query: 154 DGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQE----WM 213
           D   D  T+ +G+RNV    KAL E  RVLK G +   L+ +    P    + +     +
Sbjct: 168 DNSMDGYTIAFGIRNVTHIEKALAEAYRVLKRGGRFLCLELSHVEIPVFKNLYDLYSFQV 227

Query: 214 INNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLV 256
           I N+   +A   G  E Y+YL +S++ F   +    +  + GF   + YE    L+G +V
Sbjct: 228 IPNLGELIA---GDRESYQYLVESVRRFPPQERFASMIADAGFE-KVEYE---NLVGGVV 281

BLAST of MC08g0034 vs. TAIR 10
Match: AT5G57300.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 3.2e-22
Identity = 70/241 (29.05%), Postives = 111/241 (46.06%), Query Frame = 0

Query: 34  LFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWSGAKPGDRVLDICCGSGDLAFLLSERLGS 93
           +F  +A  YD +ND++S G HR+WK   V       G + LD+  G+GD+AF + + + S
Sbjct: 48  VFTNVASSYDIMNDVMSGGLHRLWKERLVGKLSPFAGMKHLDVAGGTGDVAFRIYDAVYS 107

Query: 94  ---------------DGKVTGLDFSREQLIIASSRQRSLSKACYKNIEWVEGDALNLPFS 153
                          + ++   D +   L +   R         K++ WVEGDA  L F 
Sbjct: 108 VKRRALQKVDEASLEETQIYVCDINPNMLNVGKQRAAERGLRDNKSLVWVEGDAEALSFD 167

Query: 154 DGYFDAITMGYGLRNVVDKRKALEEMLRVLKEGSKVSILDFNKTTQPTAAAIQE----WM 213
           D   D  T+ +G+RNV    KAL E  RVLK G +   L+ +    P    + +     +
Sbjct: 168 DNSMDGYTIAFGIRNVTHIEKALAEAYRVLKRGGRFLCLELSHVEIPVFKNLYDLYSFQV 227

Query: 214 INNIVVPVASGYGLAEDYKYLSKSIKEFFTGKELEELALEVGFSTSIHYEISGGLMGNLV 256
           I N+   +A   G  E Y+YL +S++ F   +    +  + GF   + YE    L+G +V
Sbjct: 228 IPNLGELIA---GDRESYQYLVESVRRFPPQERFASMIADAGFE-KVEYE---NLVGGVV 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3ED659.7e-9368.672-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Arabidopsis... [more]
Q8YLP45.3e-6752.652-phytyl-1,4-naphtoquinone methyltransferase OS=Nostoc sp. (strain PCC 7120 / SA... [more]
Q3MD915.9e-6651.772-phytyl-1,4-naphtoquinone methyltransferase OS=Trichormus variabilis (strain AT... [more]
B2IUM71.7e-6554.422-phytyl-1,4-naphtoquinone methyltransferase OS=Nostoc punctiforme (strain ATCC ... [more]
P728181.1e-6453.982-phytyl-1,4-naphtoquinone methyltransferase OS=Synechocystis sp. (strain PCC 68... [more]
Match NameE-valueIdentityDescription
XP_022131612.11.27e-182100.002-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic isoform X1 [Mo... [more]
XP_022951506.15.75e-16190.272-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like isoform X... [more]
XP_023545823.11.13e-16088.722-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic isoform X3 [Cu... [more]
XP_038885206.13.25e-16089.882-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic [Benincasa his... [more]
XP_023002763.13.32e-16089.492-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic-like [Cucurbit... [more]
Match NameE-valueIdentityDescription
A0A6J1BRH76.16e-183100.002-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Momordica c... [more]
A0A6J1GHS22.78e-16190.272-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita m... [more]
A0A6J1KUI91.61e-16089.492-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita m... [more]
A0A6J1GIZ36.20e-15988.892-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Cucurbita m... [more]
A0A6J1BQR31.81e-158100.002-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic OS=Momordica c... [more]
Match NameE-valueIdentityDescription
AT1G23360.16.9e-9468.67S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23360.34.8e-5563.52S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23360.24.8e-5563.52S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G57300.13.2e-2229.05S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G57300.23.2e-2229.05S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004033UbiE/COQ5 methyltransferasePFAMPF01209Ubie_methyltrancoord: 32..250
e-value: 1.0E-55
score: 188.6
IPR004033UbiE/COQ5 methyltransferaseTIGRFAMTIGR01934TIGR01934coord: 33..255
e-value: 1.1E-65
score: 219.1
IPR004033UbiE/COQ5 methyltransferaseHAMAPMF_01813MenG_UbiE_methyltrcoord: 29..257
score: 31.486191
IPR004033UbiE/COQ5 methyltransferasePROSITEPS51608SAM_MT_UBIEcoord: 20..257
score: 53.07975
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 37..257
e-value: 2.5E-57
score: 196.0
NoneNo IPR availablePANTHERPTHR43591:SF692-PHYTYL-1,4-BETA-NAPHTHOQUINONE METHYLTRANSFERASE, CHLOROPLASTICcoord: 14..257
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 14..257
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 72..176
e-value: 1.44085E-14
score: 65.9142
IPR023576UbiE/COQ5 methyltransferase, conserved sitePROSITEPS01183UBIE_1coord: 42..57
IPR0329042-phytyl-1,4-naphtoquinone methyltransferase MenGHAMAPMF_01982MenG_phylloquinone_subfamcoord: 30..257
score: 52.16309
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 28..248

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0034.1MC08g0034.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0042372 phylloquinone biosynthetic process
biological_process GO:0006744 ubiquinone biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0052624 2-phytyl-1,4-naphthoquinone methyltransferase activity
molecular_function GO:0008168 methyltransferase activity