MC08g0031 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g0031 vs. ExPASy Swiss-Prot
Match: Q9LDE3 (F-box/kelch-repeat protein At1g23390 OS=Arabidopsis thaliana OX=3702 GN=At1g23390 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.0e-47
Identity = 132/382 (34.55%), Postives = 204/382 (53.40%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSAT-- 60
           I GD+LE I+  L L+DL  A +VS +W  A+  SL+RL T+ PWL +++Q ++   T  
Sbjct: 20  IDGDILESILSYLPLLDLDSACQVSKSWNRAVFYSLRRLKTM-PWLFVYNQRNSPPYTMA 79

Query: 61  ARAVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAP 120
             A+AYDP+S  W+E+     P  + S  RSSHS+LLY +S +RF++S D  HL W    
Sbjct: 80  TMAMAYDPKSEAWIELNTASSPVEHVSVARSSHSTLLYALSPARFSFSTDAFHLTWQHVA 139

Query: 121 TPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMP 180
            P +    WR DP+VA VG+ L+I    C     +D     +   ++    WE  C+SMP
Sbjct: 140 PPRV----WRIDPIVAVVGRSLIIAGGVCD--FEEDRFAVELFDIESGDGAWE-RCESMP 199

Query: 181 TQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGE 240
             L +S +STWLSVAV+       MYV+ K SGVT SF+P +RSW+   D+       GE
Sbjct: 200 DFLYESASSTWLSVAVS----SEKMYVTEKRSGVTCSFNPVTRSWTKLLDL-----CPGE 259

Query: 241 SVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEV--------EGEGVCSWRMPAELLEK 300
              YS     S + +I  G+ G+  + + + +WEV        + E + S  MP   LEK
Sbjct: 260 CSLYSRSIGFSVNRLIMAGIIGDEYNPTGIELWEVIDSDESHLKFESIGS--MPETYLEK 319

Query: 301 LKGDSAGY-MPSISVNVMDDFVFIHNPLQ-PREVIVCHLH-LRTCRWGSVCVGVAAFDDA 360
           L+G ++ + + SI +N + D V++H   +   E++   +   + C+W ++    A +  +
Sbjct: 320 LRGINSDWPLTSIVLNAVGDMVYVHGAAENGGEIVAAEIEGGKLCKWRTLPNADATWKKS 379

Query: 361 CLFRSLVFAASTVTLPHLELAF 370
                ++ A S V    L+LAF
Sbjct: 380 HAAERVIVACSNVGFSDLKLAF 382

BLAST of MC08g0031 vs. NCBI nr
Match: XP_022132042.1 (F-box/kelch-repeat protein At1g23390 [Momordica charantia])

HSP 1 Score: 766 bits (1978), Expect = 2.39e-279
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR
Sbjct: 17  IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 76

Query: 61  AVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAPTP 120
           AVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAPTP
Sbjct: 77  AVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAPTP 136

Query: 121 PMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMPTQ 180
           PMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMPTQ
Sbjct: 137 PMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMPTQ 196

Query: 181 LMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGESV 240
           LMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGESV
Sbjct: 197 LMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGESV 256

Query: 241 FYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEVEGEGVCSWRMPAELLEKLKGDSAGYMP 300
           FYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEVEGEGVCSWRMPAELLEKLKGDSAGYMP
Sbjct: 257 FYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEVEGEGVCSWRMPAELLEKLKGDSAGYMP 316

Query: 301 SISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFDDACLFRSLVFAASTV 360
           SISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFDDACLFRSLVFAASTV
Sbjct: 317 SISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFDDACLFRSLVFAASTV 376

Query: 361 TLPHLELAF 369
           TLPHLELAF
Sbjct: 377 TLPHLELAF 385

BLAST of MC08g0031 vs. NCBI nr
Match: XP_022962361.1 (F-box/kelch-repeat protein At1g23390-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 418 bits (1074), Expect = 6.51e-142
Identity = 227/378 (60.05%), Postives = 279/378 (73.81%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGD+LEEI+  + LVDLV AN V+S+WRSAISTSL RLNT+KPWL++HSQ + + +   
Sbjct: 10  IGGDILEEIISHVPLVDLVSANSVASSWRSAISTSLLRLNTVKPWLMLHSQSTRSPSAVT 69

Query: 61  AVAYDPRSHLWMEIRKPPPPAG------YFSPLRSSHSSLLYMISSSRFAYSVDPLHLHW 120
            +AYDPRSHLWME+++P   +       YFS LRSS SSLLYM+S+SRFA+S+DPLHL+W
Sbjct: 70  TLAYDPRSHLWMEVQQPSSSSNSSSSIHYFSLLRSSPSSLLYMLSASRFAFSMDPLHLNW 129

Query: 121 HEAPTPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPC 180
            E   PP+G   WRPDPVVAAVG H+V+  +G    + DD T+PP++IYDTTT TWE  C
Sbjct: 130 -EVAAPPIG---WRPDPVVAAVGNHIVVAGSGAYCGIQDDDTIPPLEIYDTTTGTWEW-C 189

Query: 181 DSMPTQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGG 240
           DSMPT+LMDS ASTWLSVA    A+++ MYVSVK SGVT+SFDPR+++W G Y+VR   G
Sbjct: 190 DSMPTELMDSAASTWLSVA----ADQNKMYVSVKRSGVTYSFDPRTKTWCGPYNVR---G 249

Query: 241 GGGESVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWE--VEGEGVCS-WRMPAELLEKL 300
              ESVF+S I  C G G+I VGL GEAE V SVVMWE  VE EG    W MP +L+EKL
Sbjct: 250 DDVESVFFSVIG-CGGKGMIVVGLRGEAEDVRSVVMWELLVETEGRRDEWEMPFQLVEKL 309

Query: 301 KGDSAGYMPSISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFD-DACLF 360
           KG+S  YMPSI +NVM DFVF+HNPL+ REV+V  +    CRWGSV    AA D D+C F
Sbjct: 310 KGESE-YMPSIHLNVMGDFVFVHNPLEAREVVVYEVKEGACRWGSVTA--AALDRDSCRF 369

Query: 361 RSLVFAASTVTLPHLELA 368
           R+LV + S VTLP+LE A
Sbjct: 370 RNLVVSCSNVTLPYLERA 371

BLAST of MC08g0031 vs. NCBI nr
Match: XP_023547142.1 (F-box/kelch-repeat protein At1g23390-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417 bits (1073), Expect = 2.24e-141
Identity = 226/381 (59.32%), Postives = 279/381 (73.23%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGD+LEEI+  + LVDLV AN V+S+WRSAIS+SL RLNT+KPWL++HSQ + + +   
Sbjct: 33  IGGDILEEIISHVPLVDLVSANSVASSWRSAISSSLLRLNTVKPWLMLHSQSTRSPSAVT 92

Query: 61  AVAYDPRSHLWMEIRKPPPPAG---------YFSPLRSSHSSLLYMISSSRFAYSVDPLH 120
            +AYDPRSHLWME+++P   +          YFS LRSS SSLLYM+S+SRFA+S+DPLH
Sbjct: 93  TLAYDPRSHLWMEVQQPSSSSSSSSSSSSIHYFSLLRSSPSSLLYMLSASRFAFSMDPLH 152

Query: 121 LHWHEAPTPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWE 180
           L+W  A  PP+G   WRPDPVVAAVG H+++  +G    + DD T+PP++IYDTTT TWE
Sbjct: 153 LNWQVA-APPIG---WRPDPVVAAVGNHIIVAGSGACCGIQDDDTIPPLEIYDTTTGTWE 212

Query: 181 CPCDSMPTQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRR 240
             CDSMPT+LMDS ASTWLSVA    AE++ MYVSVK SGVT+SFDPR+++W G Y+VR 
Sbjct: 213 W-CDSMPTELMDSAASTWLSVA----AEQNKMYVSVKRSGVTYSFDPRTKTWCGPYNVR- 272

Query: 241 RGGGGGESVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWE--VEGEGVCS-WRMPAELL 300
             G   ESVF+S I  C G G+I VGL GEAE V SVVMWE  VE EG    W MP +L+
Sbjct: 273 --GDDVESVFFSVIG-CGGKGMIVVGLRGEAEDVRSVVMWELLVETEGRRDEWEMPFQLV 332

Query: 301 EKLKGDSAGYMPSISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFD-DA 360
           EKLKG+S  YMPSI +NVM DFVF+HNP + REV+VC +    CRWGSV    AA D D+
Sbjct: 333 EKLKGESE-YMPSIHLNVMGDFVFVHNPSEAREVVVCEVKEGACRWGSVTA--AALDRDS 392

Query: 361 CLFRSLVFAASTVTLPHLELA 368
           C FR+LV + S VTLP+LE A
Sbjct: 393 CRFRNLVVSCSNVTLPYLERA 397

BLAST of MC08g0031 vs. NCBI nr
Match: XP_022962360.1 (F-box/kelch-repeat protein At1g23390-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 416 bits (1070), Expect = 3.02e-141
Identity = 227/382 (59.42%), Postives = 279/382 (73.04%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGD+LEEI+  + LVDLV AN V+S+WRSAISTSL RLNT+KPWL++HSQ + + +   
Sbjct: 10  IGGDILEEIISHVPLVDLVSANSVASSWRSAISTSLLRLNTVKPWLMLHSQSTRSPSAVT 69

Query: 61  AVAYDPRSHLWMEIRKPPPPAG----------YFSPLRSSHSSLLYMISSSRFAYSVDPL 120
            +AYDPRSHLWME+++P   +           YFS LRSS SSLLYM+S+SRFA+S+DPL
Sbjct: 70  TLAYDPRSHLWMEVQQPSSSSSSSSSSSSSIHYFSLLRSSPSSLLYMLSASRFAFSMDPL 129

Query: 121 HLHWHEAPTPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATW 180
           HL+W E   PP+G   WRPDPVVAAVG H+V+  +G    + DD T+PP++IYDTTT TW
Sbjct: 130 HLNW-EVAAPPIG---WRPDPVVAAVGNHIVVAGSGAYCGIQDDDTIPPLEIYDTTTGTW 189

Query: 181 ECPCDSMPTQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVR 240
           E  CDSMPT+LMDS ASTWLSVA    A+++ MYVSVK SGVT+SFDPR+++W G Y+VR
Sbjct: 190 EW-CDSMPTELMDSAASTWLSVA----ADQNKMYVSVKRSGVTYSFDPRTKTWCGPYNVR 249

Query: 241 RRGGGGGESVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWE--VEGEGVCS-WRMPAEL 300
              G   ESVF+S I  C G G+I VGL GEAE V SVVMWE  VE EG    W MP +L
Sbjct: 250 ---GDDVESVFFSVIG-CGGKGMIVVGLRGEAEDVRSVVMWELLVETEGRRDEWEMPFQL 309

Query: 301 LEKLKGDSAGYMPSISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFD-D 360
           +EKLKG+S  YMPSI +NVM DFVF+HNPL+ REV+V  +    CRWGSV    AA D D
Sbjct: 310 VEKLKGESE-YMPSIHLNVMGDFVFVHNPLEAREVVVYEVKEGACRWGSVTA--AALDRD 369

Query: 361 ACLFRSLVFAASTVTLPHLELA 368
           +C FR+LV + S VTLP+LE A
Sbjct: 370 SCRFRNLVVSCSNVTLPYLERA 375

BLAST of MC08g0031 vs. NCBI nr
Match: XP_022996915.1 (F-box/kelch-repeat protein At1g23390-like [Cucurbita maxima])

HSP 1 Score: 416 bits (1070), Expect = 5.77e-141
Identity = 225/378 (59.52%), Postives = 279/378 (73.81%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGD+LEEI+  + LVDLV AN V+S+WRSAIS+SL RLNT+KPWL++HSQ + + +   
Sbjct: 33  IGGDILEEIISHVPLVDLVSANSVASSWRSAISSSLLRLNTVKPWLMLHSQSTRSPSAVT 92

Query: 61  AVAYDPRSHLWMEIRKP------PPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHW 120
            +AYDPRSHLWME+++P           YFS LRSS SSLLYM+S+SRFA+S+DPLHL+W
Sbjct: 93  TLAYDPRSHLWMEVQQPCSFSNSSSSIHYFSLLRSSPSSLLYMLSASRFAFSMDPLHLNW 152

Query: 121 HEAPTPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPC 180
             A  PP+G   WRPDPVVAAVG H+++  +G    + DD T+PP++IYDTTT TWE  C
Sbjct: 153 QVA-APPIG---WRPDPVVAAVGNHIIVAGSGACCGIQDDDTIPPLEIYDTTTGTWEW-C 212

Query: 181 DSMPTQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGG 240
           +SMPT+LMDS ASTWLSVA    A+++ MYVSVK SGVT+SFDPR+++W G Y+VR   G
Sbjct: 213 NSMPTELMDSAASTWLSVA----ADQNKMYVSVKRSGVTYSFDPRTKTWCGPYNVR---G 272

Query: 241 GGGESVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWE--VEGEGVCS-WRMPAELLEKL 300
              ESVF+S I  C G G+I VGL GEAE V SVVMWE  VE EG    W MP +L+EKL
Sbjct: 273 DDVESVFFSVIG-CGGKGMIVVGLRGEAEDVRSVVMWELLVETEGRRDEWEMPFQLVEKL 332

Query: 301 KGDSAGYMPSISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFD-DACLF 360
           KG+S  YMPSI +NVM DFVF+HNPL+ REV+VC +    CRWGSV    AA D D+C F
Sbjct: 333 KGESE-YMPSIHLNVMGDFVFVHNPLEAREVVVCEVKEGACRWGSVTA--AALDRDSCRF 392

Query: 361 RSLVFAASTVTLPHLELA 368
           R+LV + S VTLP+LE A
Sbjct: 393 RNLVVSCSNVTLPYLERA 394

BLAST of MC08g0031 vs. ExPASy TrEMBL
Match: A0A6J1BSR4 (F-box/kelch-repeat protein At1g23390 OS=Momordica charantia OX=3673 GN=LOC111005008 PE=4 SV=1)

HSP 1 Score: 766 bits (1978), Expect = 1.16e-279
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR
Sbjct: 17  IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 76

Query: 61  AVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAPTP 120
           AVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAPTP
Sbjct: 77  AVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAPTP 136

Query: 121 PMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMPTQ 180
           PMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMPTQ
Sbjct: 137 PMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMPTQ 196

Query: 181 LMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGESV 240
           LMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGESV
Sbjct: 197 LMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGESV 256

Query: 241 FYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEVEGEGVCSWRMPAELLEKLKGDSAGYMP 300
           FYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEVEGEGVCSWRMPAELLEKLKGDSAGYMP
Sbjct: 257 FYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEVEGEGVCSWRMPAELLEKLKGDSAGYMP 316

Query: 301 SISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFDDACLFRSLVFAASTV 360
           SISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFDDACLFRSLVFAASTV
Sbjct: 317 SISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFDDACLFRSLVFAASTV 376

Query: 361 TLPHLELAF 369
           TLPHLELAF
Sbjct: 377 TLPHLELAF 385

BLAST of MC08g0031 vs. ExPASy TrEMBL
Match: A0A6J1HCW8 (F-box/kelch-repeat protein At1g23390-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462829 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 3.15e-142
Identity = 227/378 (60.05%), Postives = 279/378 (73.81%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGD+LEEI+  + LVDLV AN V+S+WRSAISTSL RLNT+KPWL++HSQ + + +   
Sbjct: 10  IGGDILEEIISHVPLVDLVSANSVASSWRSAISTSLLRLNTVKPWLMLHSQSTRSPSAVT 69

Query: 61  AVAYDPRSHLWMEIRKPPPPAG------YFSPLRSSHSSLLYMISSSRFAYSVDPLHLHW 120
            +AYDPRSHLWME+++P   +       YFS LRSS SSLLYM+S+SRFA+S+DPLHL+W
Sbjct: 70  TLAYDPRSHLWMEVQQPSSSSNSSSSIHYFSLLRSSPSSLLYMLSASRFAFSMDPLHLNW 129

Query: 121 HEAPTPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPC 180
            E   PP+G   WRPDPVVAAVG H+V+  +G    + DD T+PP++IYDTTT TWE  C
Sbjct: 130 -EVAAPPIG---WRPDPVVAAVGNHIVVAGSGAYCGIQDDDTIPPLEIYDTTTGTWEW-C 189

Query: 181 DSMPTQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGG 240
           DSMPT+LMDS ASTWLSVA    A+++ MYVSVK SGVT+SFDPR+++W G Y+VR   G
Sbjct: 190 DSMPTELMDSAASTWLSVA----ADQNKMYVSVKRSGVTYSFDPRTKTWCGPYNVR---G 249

Query: 241 GGGESVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWE--VEGEGVCS-WRMPAELLEKL 300
              ESVF+S I  C G G+I VGL GEAE V SVVMWE  VE EG    W MP +L+EKL
Sbjct: 250 DDVESVFFSVIG-CGGKGMIVVGLRGEAEDVRSVVMWELLVETEGRRDEWEMPFQLVEKL 309

Query: 301 KGDSAGYMPSISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFD-DACLF 360
           KG+S  YMPSI +NVM DFVF+HNPL+ REV+V  +    CRWGSV    AA D D+C F
Sbjct: 310 KGESE-YMPSIHLNVMGDFVFVHNPLEAREVVVYEVKEGACRWGSVTA--AALDRDSCRF 369

Query: 361 RSLVFAASTVTLPHLELA 368
           R+LV + S VTLP+LE A
Sbjct: 370 RNLVVSCSNVTLPYLERA 371

BLAST of MC08g0031 vs. ExPASy TrEMBL
Match: A0A6J1HEU1 (F-box/kelch-repeat protein At1g23390-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462829 PE=4 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 1.46e-141
Identity = 227/382 (59.42%), Postives = 279/382 (73.04%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGD+LEEI+  + LVDLV AN V+S+WRSAISTSL RLNT+KPWL++HSQ + + +   
Sbjct: 10  IGGDILEEIISHVPLVDLVSANSVASSWRSAISTSLLRLNTVKPWLMLHSQSTRSPSAVT 69

Query: 61  AVAYDPRSHLWMEIRKPPPPAG----------YFSPLRSSHSSLLYMISSSRFAYSVDPL 120
            +AYDPRSHLWME+++P   +           YFS LRSS SSLLYM+S+SRFA+S+DPL
Sbjct: 70  TLAYDPRSHLWMEVQQPSSSSSSSSSSSSSIHYFSLLRSSPSSLLYMLSASRFAFSMDPL 129

Query: 121 HLHWHEAPTPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATW 180
           HL+W E   PP+G   WRPDPVVAAVG H+V+  +G    + DD T+PP++IYDTTT TW
Sbjct: 130 HLNW-EVAAPPIG---WRPDPVVAAVGNHIVVAGSGAYCGIQDDDTIPPLEIYDTTTGTW 189

Query: 181 ECPCDSMPTQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVR 240
           E  CDSMPT+LMDS ASTWLSVA    A+++ MYVSVK SGVT+SFDPR+++W G Y+VR
Sbjct: 190 EW-CDSMPTELMDSAASTWLSVA----ADQNKMYVSVKRSGVTYSFDPRTKTWCGPYNVR 249

Query: 241 RRGGGGGESVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWE--VEGEGVCS-WRMPAEL 300
              G   ESVF+S I  C G G+I VGL GEAE V SVVMWE  VE EG    W MP +L
Sbjct: 250 ---GDDVESVFFSVIG-CGGKGMIVVGLRGEAEDVRSVVMWELLVETEGRRDEWEMPFQL 309

Query: 301 LEKLKGDSAGYMPSISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFD-D 360
           +EKLKG+S  YMPSI +NVM DFVF+HNPL+ REV+V  +    CRWGSV    AA D D
Sbjct: 310 VEKLKGESE-YMPSIHLNVMGDFVFVHNPLEAREVVVYEVKEGACRWGSVTA--AALDRD 369

Query: 361 ACLFRSLVFAASTVTLPHLELA 368
           +C FR+LV + S VTLP+LE A
Sbjct: 370 SCRFRNLVVSCSNVTLPYLERA 375

BLAST of MC08g0031 vs. ExPASy TrEMBL
Match: A0A6J1K837 (F-box/kelch-repeat protein At1g23390-like OS=Cucurbita maxima OX=3661 GN=LOC111492006 PE=4 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 2.79e-141
Identity = 225/378 (59.52%), Postives = 279/378 (73.81%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           IGGD+LEEI+  + LVDLV AN V+S+WRSAIS+SL RLNT+KPWL++HSQ + + +   
Sbjct: 33  IGGDILEEIISHVPLVDLVSANSVASSWRSAISSSLLRLNTVKPWLMLHSQSTRSPSAVT 92

Query: 61  AVAYDPRSHLWMEIRKP------PPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHW 120
            +AYDPRSHLWME+++P           YFS LRSS SSLLYM+S+SRFA+S+DPLHL+W
Sbjct: 93  TLAYDPRSHLWMEVQQPCSFSNSSSSIHYFSLLRSSPSSLLYMLSASRFAFSMDPLHLNW 152

Query: 121 HEAPTPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPC 180
             A  PP+G   WRPDPVVAAVG H+++  +G    + DD T+PP++IYDTTT TWE  C
Sbjct: 153 QVA-APPIG---WRPDPVVAAVGNHIIVAGSGACCGIQDDDTIPPLEIYDTTTGTWEW-C 212

Query: 181 DSMPTQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGG 240
           +SMPT+LMDS ASTWLSVA    A+++ MYVSVK SGVT+SFDPR+++W G Y+VR   G
Sbjct: 213 NSMPTELMDSAASTWLSVA----ADQNKMYVSVKRSGVTYSFDPRTKTWCGPYNVR---G 272

Query: 241 GGGESVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWE--VEGEGVCS-WRMPAELLEKL 300
              ESVF+S I  C G G+I VGL GEAE V SVVMWE  VE EG    W MP +L+EKL
Sbjct: 273 DDVESVFFSVIG-CGGKGMIVVGLRGEAEDVRSVVMWELLVETEGRRDEWEMPFQLVEKL 332

Query: 301 KGDSAGYMPSISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFD-DACLF 360
           KG+S  YMPSI +NVM DFVF+HNPL+ REV+VC +    CRWGSV    AA D D+C F
Sbjct: 333 KGESE-YMPSIHLNVMGDFVFVHNPLEAREVVVCEVKEGACRWGSVTA--AALDRDSCRF 392

Query: 361 RSLVFAASTVTLPHLELA 368
           R+LV + S VTLP+LE A
Sbjct: 393 RNLVVSCSNVTLPYLERA 394

BLAST of MC08g0031 vs. ExPASy TrEMBL
Match: A0A2N9IQU5 (F-box domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS54412 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 7.56e-96
Identity = 178/375 (47.47%), Postives = 230/375 (61.33%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSATAR 60
           I GD+LE I+  + L+DLV A  VS+ W+ A+ +SLK LN +KPWL++H+Q + N  +  
Sbjct: 15  IHGDILEAILSHVPLIDLVPACHVSTTWKGAVFSSLKHLNPIKPWLIVHAQTNRNPYSTT 74

Query: 61  AVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAPTP 120
             AYDPRSH+W+EI +P     Y S LRSSHSSLLYM S  RF +S DPLHL WH+A   
Sbjct: 75  THAYDPRSHVWVEIDQPS--IQYVSTLRSSHSSLLYMQSPFRFVFSSDPLHLTWHKA--- 134

Query: 121 PMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMPTQ 180
            +    WRPDP+VA VG+ +VI     G+C   D  +  V+ YD  T  W+  CDSMP  
Sbjct: 135 -VAQLVWRPDPIVAMVGQRVVIAG---GACDFGDDPLA-VETYDLKTCKWDT-CDSMPGI 194

Query: 181 LMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGESV 240
           L DS ASTWLSVAV    +E  MYV+ K SGV  SFDP +++W G YD+R      G++V
Sbjct: 195 LKDSAASTWLSVAV----DEERMYVTEKCSGVMHSFDPITKTWRGPYDLRP-----GQNV 254

Query: 241 FYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEVEGE------GVCSWRMPAELLEKLKGD 300
           F S I + +   +I VGL GEAE+V SV +WEV+GE       +    MP ELL K KG 
Sbjct: 255 FSSVIGFVNDR-MIVVGLIGEAENVKSVKLWEVKGEMLEIKEEIEMGEMPKELLVKFKGQ 314

Query: 301 SAGYMPSISVNVMDDFVFIHNPLQPREVIVCHLHLRTCRWGSVCVGVAAFDDACLFRSLV 360
           S   + SI++N M +FVFIHNP  P EVIVC +    C WGSV   V   D + + +SLV
Sbjct: 315 SPR-VSSIAMNSMGNFVFIHNPSDPAEVIVCEVVNGACNWGSV-RNVVVNDGSRIMQSLV 366

Query: 361 FAASTVTLPHLELAF 369
           +  + V L  L  AF
Sbjct: 375 YTCANVGLGDLNQAF 366

BLAST of MC08g0031 vs. TAIR 10
Match: AT1G23390.1 (Kelch repeat-containing F-box family protein )

HSP 1 Score: 190.3 bits (482), Expect = 2.8e-48
Identity = 132/382 (34.55%), Postives = 204/382 (53.40%), Query Frame = 0

Query: 1   IGGDMLEEIVCRLALVDLVWANRVSSAWRSAISTSLKRLNTLKPWLVIHSQYSTNSAT-- 60
           I GD+LE I+  L L+DL  A +VS +W  A+  SL+RL T+ PWL +++Q ++   T  
Sbjct: 20  IDGDILESILSYLPLLDLDSACQVSKSWNRAVFYSLRRLKTM-PWLFVYNQRNSPPYTMA 79

Query: 61  ARAVAYDPRSHLWMEIRKPPPPAGYFSPLRSSHSSLLYMISSSRFAYSVDPLHLHWHEAP 120
             A+AYDP+S  W+E+     P  + S  RSSHS+LLY +S +RF++S D  HL W    
Sbjct: 80  TMAMAYDPKSEAWIELNTASSPVEHVSVARSSHSTLLYALSPARFSFSTDAFHLTWQHVA 139

Query: 121 TPPMGGWQWRPDPVVAAVGKHLVIVAAGCGSCLIDDHTVPPVQIYDTTTATWECPCDSMP 180
            P +    WR DP+VA VG+ L+I    C     +D     +   ++    WE  C+SMP
Sbjct: 140 PPRV----WRIDPIVAVVGRSLIIAGGVCD--FEEDRFAVELFDIESGDGAWE-RCESMP 199

Query: 181 TQLMDSGASTWLSVAVAVAAEEHTMYVSVKSSGVTFSFDPRSRSWSGGYDVRRRGGGGGE 240
             L +S +STWLSVAV+       MYV+ K SGVT SF+P +RSW+   D+       GE
Sbjct: 200 DFLYESASSTWLSVAVS----SEKMYVTEKRSGVTCSFNPVTRSWTKLLDL-----CPGE 259

Query: 241 SVFYSEIAWCSGSGVIAVGLTGEAESVSSVVMWEV--------EGEGVCSWRMPAELLEK 300
              YS     S + +I  G+ G+  + + + +WEV        + E + S  MP   LEK
Sbjct: 260 CSLYSRSIGFSVNRLIMAGIIGDEYNPTGIELWEVIDSDESHLKFESIGS--MPETYLEK 319

Query: 301 LKGDSAGY-MPSISVNVMDDFVFIHNPLQ-PREVIVCHLH-LRTCRWGSVCVGVAAFDDA 360
           L+G ++ + + SI +N + D V++H   +   E++   +   + C+W ++    A +  +
Sbjct: 320 LRGINSDWPLTSIVLNAVGDMVYVHGAAENGGEIVAAEIEGGKLCKWRTLPNADATWKKS 379

Query: 361 CLFRSLVFAASTVTLPHLELAF 370
                ++ A S V    L+LAF
Sbjct: 380 HAAERVIVACSNVGFSDLKLAF 382