MC07g_new0325 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g_new0325
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DNA polymerase
Location	MC07: 11785867 .. 11789840 (+)
RNA-Seq Expression	MC07g_new0325
Synteny	MC07g_new0325

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTGCATAATTTTAGTGCACTGACAAGTACTTGTCTCATGAATTGATTATTTTCCACACGACAGGTAATTTATGGCGATACTGATTCAATAATGATTCATAGTGGACTTGATGATATTGGCAAAGCGAAAGCAATTGCAGGGAAAGTGATACAGGAGGTGAGGTTGTGTGGTCTGATTGAATTTTCCAAAGTATTTATCGTAGCAATCACGTTAGAAACTCTCATGATTTAAAATCTAAGCCTTATAAACTTCTCTTATGTTTTAGTATCGAATTATAGCGTTATTGAAAATTGTGGAAGTTTCCGTTCTATTCCTTCTCTTTTACTCTTATTAACTGGCTATTGTATAAGAATGGATAGTAATAAAAAAGAAGGTTCAACACCTTGTGGGTCTTGAAAATTCCCTCGCAAAATACTAGAAAATAGATTTTAGGTCCATCTAATTTATTCGTAATATATTGGAATGCTTGTATGACACAACCCAACAAGTTCCCGTGGCGTAGATCAGACAACAGAAGAATTTTTATCCATGTAAAAAATCTAAATCTTTCCGCTCATGGTCTCTAAGTCTCAATGTGAGGTATTCTTTCATGTTTCTCTTTTTCTAGGTCAATAGAAAATACAAGTGTTTAGAAATTGATCTCGATGGTTTGTACAAGAGAATGCTGCTTCTGAAGAAAAAGAAATATGCAGCTGTAAAGTTGCAGTGCAAGGAAGGAATGCCGTATGAGGTAACTATCATATACTTTCATTTGCTCTGTTAGTTCTCGACTGAAATAAATTGGAGCTAGAGCAGTGTCAAAGTTTACTTCGTTTCATATGTACAGGTTATTGAGCGCAAGGGTCTTGATATGGTTCGCCGTGACTGGTGTTTATTATCAAAGGAATTAGGAGATTTCTGCTTGGGTCAAATATTGTCTGGAGGGTATGTAACGTTGCCACCTTGAGAGTTTTATTTACTATTCTACGGAATTTATTTATTTGGAAACTGCATTAATTTTACTCTAACCACCCCCCACCTTTCCCCATGCTTATAGGTCATGTGATGATGTCATTGAGTCAATACATGACTCTCTTAGGAAGGTAAAAGTTGTACCTTTTCATGACTACTTTGTTGATTGTTCTTTGATAATCAATAAAGATAGAGTTTAGGTTGGTGGTCAGAAAGTTCCTACAATTTAAATTGGGGTGTGTGATAAATTGGCAATGATCCTGAAACAACTCTGAAAAATGTGGACACGTAACAAGTGACACCCCCCTCGTGCGCACACTATGAAATTAAAGAAATAAAAGCACCAACACTACTCAGGAAAGGGCGAAATATAATAGAAAAATGAGCCTTTGGATGTCTGTAATTATTGGGAATTCTTTCGGATTAAATATGCAAACTTTACACAGATACAAGATGATATTAGGAGAGGGCAAGTAGCACTTGAGAAATATATCATCAGGAAGACATTGACTAAGCCACCTGAAGCCTATCCTGATGCCAGAAACCAACCACATGTTCAAGTAAGCAAATGCTTAAACCACTGCAAATTATTTTATCTACCATTACTTTTGTTTTGAAGATGACGTCTGTGTAATGTACAGGTTGCGCAAAGGTTAAAACAAATAGGTTATCTTACGGGCTGTTATGTTGGTGATACGATCCCGTATATAATTTGCTGTGAGCAGGTTTGTGCAAGTGGTTTAGTATTTATTTCCTAGATATGATGCTATAACCGTAGCTGTGTTTATAATATAACTATTTTATGGTGAAATCTAAAAGTTGGAAGAACATTGTTACTTTATTATTTTCCTTTCCATTTTTTTCTTTGCTTTGCTTTTTCGGCCAGGGATCTACTTCTGGTGGTTCTACAGGCATTGCTCAGCGGGCTAGACATCCTGAAGAACTTAAAAGAGAAGGTGGAAAGTGGATGATTGATACTGATTACTACTTGTCACAGCAGGTCTTTTTTCTTCATGTTTTTGCTTTTCCACGAATAATCATGATCTGTTGTTACTGTCATTCTATAATGATTTCATGTTGTTGACATATGAACGAATAGATACACCCTGTGGTCTCTCGTCTATGTGCCTCAATTCAGGGCACAAGCCCAGAATGCTTGGCTCACTGTCTGGGGCTTGATCCATCGAAGGTAAAACATGATCCTGCTTACTGGAGGAAAAAAGAAAAGAAAAGAAAAGAAAAAGTTGGGGTTTCCTCACTTTCTAAATATTACATGCACACTTCTGAGGTACTATACTTAATTATGTTCAGTTCAAGATCAAATCATGTGAAGTTTTCAGCAGCGATGTCTCCTCTTCCCTCGTGTCCTGCGTAACTGATGAGCAAAGGTCATGATTCGAATTGTTAAAACTTCACATAACTTCAAACTAATCCTTGAATTTCCCTTGGAAACTTGTTATAAGTTGCGTGGATTCTAAAATAATAACATTGCTTTTGTTTGACCACCTACAGGTACCTGGACTGTAAACCACTGGTATTGACTTGCCCTAGGTGTTATGGTACTTTCGAAGTTCCTACTATCTTCAGTTCTATATACAAATCAACATATGGAAAGCAAGAAAGACCAGTGGTTGATGAACCTACAAAAAAGTTTTGGAGTAATTTCAAATGTCCAAACTGCAAGGATTTGTTATGGGTCCCTGACGAAGCTAATGCTACGGGTAGGGGTAGAATGACGTGTGGAATGATTTCCAACCAGGTAGGTGCCTATGAGACTACTTTTTCCAAATAACAATTAATCATATTGCTCATTCCAGAGAACATAGTACCAAGAGTTGCTTTTGAATCAGCAAATAGTTTTAAACGCTACAAGATCAGGATAGTTTTAAATTCTGGATCAAATTTGGTATAACCCCATAGAGTGTGCTCTCAAAATTTGACTGACTTGTTATTGTTATGCGTCTCTTGAGAGGACATGTTTCAATTTGGCAATTTTGTAACAATATCCAAATGTAACATGTTACAGGTAAAAATGCAAACAGACAGGTTCATTGCAAAGTATTATCATGGCTTAATGATGGTAATTTACCTCTTAGAAATCCTTTTTTTGGGCTGATTATATAGTTATTGAAGTGGTGTAAATCTAAAAAATCTTTTGAATATTATAGTGCGACGAGGAAACATGCAAATACACCACATGTATTGTCAACCTTCAACGTGCGGGTGACTCCAAAAAAGAGATTCTCTGCCCAAAATATCCTCAGTGTGATGGGCGTCTCGTAAGAACGGTATTACATTATCTACTTCAAAAGGAATATGAATATAGTCCTTTTACATATTTTGATTCTTCCTAGAATTATGGCTCTAATTGTGAATGCCCCTGTATATCAGTATACTAAGGCGGACTTGTGGAAGCAGATTTGTTATTTTTGTCACGTGTTGGATACTGAACGCTGTATGGAAAAGGTTATTTGCTTCTCTATATTCACGTCATATTCTTGAGTTGAGACAATGCAATCGCTGTTAATATCTTGTTGGCTTTGTGCAACAGCTGGAGGTTCATAGAAGGGTAGCTTTAGAAAAAGAAATGGCAAAGATTCGAGCATTGGTAAAGTTGGCTGCATCGACGGTTAATAAGGTTCGATATCGCAGCGCATATGGTTCCATTAAGTTGAAGGATATTGCAGTGAGTTTGCATATGGCACCATGTTAGGCAGGCTAGATATCGAACTCATAACTTCTTGGTTGGAGATATATGTCAATTAACGTTAAGCTATACATACTTTGACAGGTTAGATATCTATTTATAATTACCATTCGTTGCATCGTATATGTTTGTCACGTGGATGAATACTGAAATAGGACATTGGGTGGAATTTTTATTCTTGCCTTCGTCTATTGTGAGTTGTTACATTATATGTAACTTTTGTTATTAATACAAGTATTTAATTTACCAATAATTTCAGCTAGATATGTTTCTACAAATTTGTGGGAAAATGATGTAACATTGGATGCATTTAAG

mRNA sequence

ATGATTCATAGTGGACTTGATGATATTGGCAAAGCGAAAGCAATTGCAGGGAAAGTGATACAGGAGGTCAATAGAAAATACAAGTGTTTAGAAATTGATCTCGATGGTTTGTACAAGAGAATGCTGCTTCTGAAGAAAAAGAAATATGCAGCTGTAAAGTTGCAGTGCAAGGAAGGAATGCCGTATGAGGTTATTGAGCGCAAGGGTCTTGATATGGTTCGCCGTGACTGGTGTTTATTATCAAAGGAATTAGGAGATTTCTGCTTGGGTCAAATATTGTCTGGAGGGTCATGTGATGATGTCATTGAGTCAATACATGACTCTCTTAGGAAGATACAAGATGATATTAGGAGAGGGCAAGTAGCACTTGAGAAATATATCATCAGGAAGACATTGACTAAGCCACCTGAAGCCTATCCTGATGCCAGAAACCAACCACATGTTCAAGTTGCGCAAAGGTTAAAACAAATAGGTTATCTTACGGGCTGTTATGTTGGTGATACGATCCCGTATATAATTTGCTGTGAGCAGGGATCTACTTCTGGTGGTTCTACAGGCATTGCTCAGCGGGCTAGACATCCTGAAGAACTTAAAAGAGAAGGTGGAAAGTGGATGATTGATACTGATTACTACTTGTCACAGCAGATACACCCTGTGGTCTCTCGTCTATGTGCCTCAATTCAGGGCACAAGCCCAGAATGCTTGGCTCACTGTCTGGGGCTTGATCCATCGAAGTTCAAGATCAAATCATGTGAAGTTTTCAGCAGCGATGTCTCCTCTTCCCTCGTGTCCTGCGTAACTGATGAGCAAAGGTACCTGGACTGTAAACCACTGGTATTGACTTGCCCTAGGTGTTATGGTACTTTCGAAGTTCCTACTATCTTCAGTTCTATATACAAATCAACATATGGAAAGCAAGAAAGACCAGTGGTTGATGAACCTACAAAAAAGTTTTGGAGTAATTTCAAATGTCCAAACTGCAAGGATTTGTTATGGGTCCCTGACGAAGCTAATGCTACGGGTAGGGGTAGAATGACGTGTGGAATGATTTCCAACCAGGTAAAAATGCAAACAGACAGGTTCATTGCAAAGTATTATCATGGCTTAATGATGTGCGACGAGGAAACATGCAAATACACCACATGTATTGTCAACCTTCAACGTGCGGGTGACTCCAAAAAAGAGATTCTCTGCCCAAAATATCCTCAGTGTGATGGGCGTCTCGTAAGAACGTATACTAAGGCGGACTTGTGGAAGCAGATTTGTTATTTTTGTCACGTGTTGGATACTGAACGCTGTATGGAAAAGCTGGAGGTTCATAGAAGGGTAGCTTTAGAAAAAGAAATGGCAAAGATTCGAGCATTGGTAAAGTTGGCTGCATCGACGGTTAATAAGGTTCGATATCGCAGCGCATATGGTTCCATTAAGTTGAAGGATATTGCAGTGAGTTTGCATATGGCACCATGTTAG

Coding sequence (CDS)

ATGATTCATAGTGGACTTGATGATATTGGCAAAGCGAAAGCAATTGCAGGGAAAGTGATACAGGAGGTCAATAGAAAATACAAGTGTTTAGAAATTGATCTCGATGGTTTGTACAAGAGAATGCTGCTTCTGAAGAAAAAGAAATATGCAGCTGTAAAGTTGCAGTGCAAGGAAGGAATGCCGTATGAGGTTATTGAGCGCAAGGGTCTTGATATGGTTCGCCGTGACTGGTGTTTATTATCAAAGGAATTAGGAGATTTCTGCTTGGGTCAAATATTGTCTGGAGGGTCATGTGATGATGTCATTGAGTCAATACATGACTCTCTTAGGAAGATACAAGATGATATTAGGAGAGGGCAAGTAGCACTTGAGAAATATATCATCAGGAAGACATTGACTAAGCCACCTGAAGCCTATCCTGATGCCAGAAACCAACCACATGTTCAAGTTGCGCAAAGGTTAAAACAAATAGGTTATCTTACGGGCTGTTATGTTGGTGATACGATCCCGTATATAATTTGCTGTGAGCAGGGATCTACTTCTGGTGGTTCTACAGGCATTGCTCAGCGGGCTAGACATCCTGAAGAACTTAAAAGAGAAGGTGGAAAGTGGATGATTGATACTGATTACTACTTGTCACAGCAGATACACCCTGTGGTCTCTCGTCTATGTGCCTCAATTCAGGGCACAAGCCCAGAATGCTTGGCTCACTGTCTGGGGCTTGATCCATCGAAGTTCAAGATCAAATCATGTGAAGTTTTCAGCAGCGATGTCTCCTCTTCCCTCGTGTCCTGCGTAACTGATGAGCAAAGGTACCTGGACTGTAAACCACTGGTATTGACTTGCCCTAGGTGTTATGGTACTTTCGAAGTTCCTACTATCTTCAGTTCTATATACAAATCAACATATGGAAAGCAAGAAAGACCAGTGGTTGATGAACCTACAAAAAAGTTTTGGAGTAATTTCAAATGTCCAAACTGCAAGGATTTGTTATGGGTCCCTGACGAAGCTAATGCTACGGGTAGGGGTAGAATGACGTGTGGAATGATTTCCAACCAGGTAAAAATGCAAACAGACAGGTTCATTGCAAAGTATTATCATGGCTTAATGATGTGCGACGAGGAAACATGCAAATACACCACATGTATTGTCAACCTTCAACGTGCGGGTGACTCCAAAAAAGAGATTCTCTGCCCAAAATATCCTCAGTGTGATGGGCGTCTCGTAAGAACGTATACTAAGGCGGACTTGTGGAAGCAGATTTGTTATTTTTGTCACGTGTTGGATACTGAACGCTGTATGGAAAAGCTGGAGGTTCATAGAAGGGTAGCTTTAGAAAAAGAAATGGCAAAGATTCGAGCATTGGTAAAGTTGGCTGCATCGACGGTTAATAAGGTTCGATATCGCAGCGCATATGGTTCCATTAAGTTGAAGGATATTGCAGTGAGTTTGCATATGGCACCATGTTAG

Protein sequence

MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGMPYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQVALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGSTSGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLGLDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYKSTYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDRFIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDIAVSLHMAPC

Homology

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BLAST of MC07g_new0325 vs. ExPASy Swiss-Prot
Match: O48653 (DNA polymerase alpha catalytic subunit OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0868300 PE=2 SV=2)

HSP 1 Score: 576.2 bits (1484), Expect = 3.4e-163
Identity = 291/486 (59.88%), Postives = 358/486 (73.66%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIH+GLDDI +AK IAGKVIQEVN+KY+CLEIDLDG+YKRMLLLKKKKYAA+K+   +G 
Sbjct: 1060 MIHTGLDDISRAKGIAGKVIQEVNKKYRCLEIDLDGIYKRMLLLKKKKYAAIKV-ALDGS 1119

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
              E IERKGLDMVRRDW LLSKE+GDFCL QILSGGSCDDVIESIH SL ++Q+ +R GQ
Sbjct: 1120 LRENIERKGLDMVRRDWSLLSKEIGDFCLNQILSGGSCDDVIESIHSSLVQVQEQMRGGQ 1179

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
              LEKYII K+LTK PE YPDA+NQPHVQVA RLKQ GY +GC  GDT+PYIIC +Q S 
Sbjct: 1180 TELEKYIITKSLTKAPEDYPDAKNQPHVQVALRLKQNGY-SGCSAGDTVPYIICSQQDSE 1239

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            S  S GIAQRARHPEELKR   KWMID DYYLSQQIHPVVSRLCASIQGTSP  LA CLG
Sbjct: 1240 STHSGGIAQRARHPEELKRNPDKWMIDIDYYLSQQIHPVVSRLCASIQGTSPARLAECLG 1299

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDE-QRYLDCKPLVLTCPRCYGTFEVPTIFSSIY 300
            LD SKF+ +  E  + D SS L+S + DE +RY  C+PL L+CP C  TF+ P + S I 
Sbjct: 1300 LDSSKFQSRLTESDNQDTSSMLLSVIDDEDERYRGCEPLRLSCPSCSTTFDCPPVSSLII 1359

Query: 301  KSTYGKQERP-VVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQT 360
             S+ G    P   ++ +  FW   +CP C      PD+   T   R++  +++NQ+K Q 
Sbjct: 1360 GSSSGNVSNPNEGNDASINFWRRMRCPRC------PDD---TDESRVSPAVLANQMKRQA 1419

Query: 361  DRFIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLW 420
            D FI  YY GL+MCD+E CKY+T  VNL+  GDS++  +CP YP+C+G LVR YT+ADL+
Sbjct: 1420 DSFINLYYKGLLMCDDEGCKYSTHSVNLRVMGDSERGTICPNYPRCNGHLVRQYTEADLY 1479

Query: 421  KQICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLK 480
            +Q+ YFC+V+D  RC+EKL+   R+  EKE A +   + LA   V K+R R A+G ++LK
Sbjct: 1480 RQLSYFCYVVDATRCLEKLDQKARLPFEKEFAALSQTINLALMEVQKIRDRCAFGWVQLK 1534

Query: 481  DIAVSL 485
            D+A+S+
Sbjct: 1540 DLAISI 1534

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BLAST of MC07g_new0325 vs. ExPASy Swiss-Prot
Match: Q9FHA3 (DNA polymerase alpha catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=POLA PE=3 SV=2)

HSP 1 Score: 572.0 bits (1473), Expect = 6.4e-162
Identity = 275/483 (56.94%), Postives = 353/483 (73.08%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDDI + KAI  KVIQEVN+KY+CL+ID DG+YKRMLLL+KKKYAAVKLQ K+G 
Sbjct: 1051 MIHSGLDDIEEVKAIKSKVIQEVNKKYRCLKIDCDGIYKRMLLLRKKKYAAVKLQFKDGK 1110

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            P E IERKG+DMVRRDW LLSKE+GD CL +IL GGSC+DV+E+IH+ L KI++++R GQ
Sbjct: 1111 PCEDIERKGVDMVRRDWSLLSKEIGDLCLSKILYGGSCEDVVEAIHNELMKIKEEMRNGQ 1170

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQG-S 180
            VALEKY+I KTLTKPP AYPD+++QPHVQVA R++Q GY  G    DT+PYIIC EQG +
Sbjct: 1171 VALEKYVITKTLTKPPAAYPDSKSQPHVQVALRMRQRGYKEGFNAKDTVPYIICYEQGNA 1230

Query: 181  TSGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCL 240
            +S  S GIA+RARHP+E+K EG +W++D DYYL+QQIHPVVSRLCA IQGTSPE LA CL
Sbjct: 1231 SSASSAGIAERARHPDEVKSEGSRWLVDIDYYLAQQIHPVVSRLCAEIQGTSPERLAECL 1290

Query: 241  GLDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIY 300
            GLDPSK++ KS +  SSD S+SL+   +DE+RY  C+PL LTCP C   F  P+I SS+ 
Sbjct: 1291 GLDPSKYRSKSNDATSSDPSTSLLFATSDEERYKSCEPLALTCPSCSTAFNCPSIISSVC 1350

Query: 301  KSTYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTD 360
             S   K   P  +E    FW    CP C+              G ++  MI+NQVK Q D
Sbjct: 1351 ASISKKPATPETEESDSTFWLKLHCPKCQ---------QEDSTGIISPAMIANQVKRQID 1410

Query: 361  RFIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWK 420
             F++ YY G+M+C++E+CK+TT   N +  G+ ++  +CP YP C+G L+R YT+ADL+K
Sbjct: 1411 GFVSMYYKGIMVCEDESCKHTTRSPNFRLLGERERGTVCPNYPNCNGTLLRKYTEADLYK 1470

Query: 421  QICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKD 480
            Q+ YFCH+LDT+  +EK++V  R+ +EK M KIR  VK AA+     R R AYG ++L D
Sbjct: 1471 QLSYFCHILDTQCSLEKMDVGVRIQVEKAMTKIRPAVKSAAAITRSSRDRCAYGWMQLTD 1524

Query: 481  IAV 483
            I +
Sbjct: 1531 IVI 1524

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BLAST of MC07g_new0325 vs. ExPASy Swiss-Prot
Match: P09884 (DNA polymerase alpha catalytic subunit OS=Homo sapiens OX=9606 GN=POLA1 PE=1 SV=2)

HSP 1 Score: 254.6 bits (649), Expect = 2.3e-66
Identity = 167/487 (34.29%), Postives = 258/487 (52.98%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ-CKEG 60
            MI++   ++ +   +  KV  EVN+ YK LEID+DG++K +LLLKKKKYAA+ ++   +G
Sbjct: 1007 MINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDG 1066

Query: 61   MPYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRG 120
                  E KGLD+VRRDWC L+K+ G+F +GQILS  S D ++E+I   L +I +++  G
Sbjct: 1067 NYVTKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNG 1126

Query: 121  QVALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGS 180
             V + ++ I K LTK P+ YPD ++ PHV VA  +   G       GDT+ Y+IC     
Sbjct: 1127 SVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGG-RKVKAGDTVSYVIC----- 1186

Query: 181  TSGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCL 240
              G +   +QRA  PE+L+++     IDT YYL+QQIHPVV+R+C  I G     +A  L
Sbjct: 1187 QDGSNLTASQRAYAPEQLQKQ-DNLTIDTQYYLAQQIHPVVARICEPIDGIDAVLIATWL 1246

Query: 241  GLDPSKFKIKSCEVFSSDVSSSLV---SCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFS 300
            GLDP++F++        + + +L+   + +TDE++Y DC+     CP C GT        
Sbjct: 1247 GLDPTQFRVH--HYHKDEENDALLGGPAQLTDEEKYRDCERFKCPCPTC-GT-------E 1306

Query: 301  SIYKSTYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKM 360
            +IY + +      +  EP+    SN  C        V                +SN++ M
Sbjct: 1307 NIYDNVFDGSGTDM--EPSLYRCSNIDCKASPLTFTV---------------QLSNKLIM 1366

Query: 361  QTDRFIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKAD 420
               RFI KYY G ++C+E TC+  T  + LQ    S+   LCP   +    L   Y+   
Sbjct: 1367 DIRRFIKKYYDGWLICEEPTCRNRTRHLPLQ---FSRTGPLCPACMK--ATLQPEYSDKS 1426

Query: 421  LWKQICYFCHVLDTERCMEKLEV-HRRVALEKEM--AKIRALVKLAASTVNKVRYRSAYG 480
            L+ Q+C++ ++ D E  +EKL   H +  L+K+    K+    +   +T  +   RS Y 
Sbjct: 1427 LYTQLCFYRYIFDAECALEKLTTDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSRSGYS 1454

[Close]

BLAST of MC07g_new0325 vs. ExPASy Swiss-Prot
Match: O89042 (DNA polymerase alpha catalytic subunit (Fragment) OS=Rattus norvegicus OX=10116 GN=Pola1 PE=1 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.1e-64
Identity = 156/460 (33.91%), Postives = 247/460 (53.70%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ-CKEG 60
            MI++   ++ +   +  KV  EVN+ YK LEID+DG++K +LLLKKKKYAA+ ++   +G
Sbjct: 1014 MINTNSTNLEEVFKLGNKVKNEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDG 1073

Query: 61   MPYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRG 120
                  E KGLD+VRRDWC L+K+ G+F +GQILS  S D ++E+I   L +I +++  G
Sbjct: 1074 NYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNG 1133

Query: 121  QVALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGS 180
             V + ++ I K LTK P+ YPD ++ PHV VA  +   G       GDT+ Y+IC     
Sbjct: 1134 SVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGG-RKVKAGDTVSYVIC----- 1193

Query: 181  TSGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCL 240
              G +    QRA  PE+L+++     IDT YYL+QQIHPVV+R+C  I G     +A  L
Sbjct: 1194 QDGSNLPATQRAYAPEQLQKQ-DNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWL 1253

Query: 241  GLDPSKFKIKSCEVFSSDVSSSLV---SCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFS 300
            GLD ++F++   +    + + +L+   + +TDE++Y DC+     CP C GT     I+ 
Sbjct: 1254 GLDSTQFRVH--QYHKDEENDALLGGPAQLTDEEKYKDCEKFKCLCPSC-GT---ENIYD 1313

Query: 301  SIYKSTYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKM 360
            ++++ +       +  EP+    SN  C        V                +SN++ M
Sbjct: 1314 NVFEGS------GMDMEPSLNRCSNIDCKASPATFMV---------------QLSNKLIM 1373

Query: 361  QTDRFIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVR-TYTKA 420
               R I KYY G ++C+E TC+     + L  + +       P  P C   ++R  Y+  
Sbjct: 1374 DIRRCIKKYYDGWLICEEPTCRNRIRRLPLHFSRNG------PLCPACMKAVLRPEYSDK 1433

Query: 421  DLWKQICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRAL 456
             L+ Q+C++ ++ D +  +EKL  H +  L+K+    R L
Sbjct: 1434 SLYTQLCFYRYIFDADCALEKLPEHEKDKLKKQFFTPRVL 1433

[Close]

BLAST of MC07g_new0325 vs. ExPASy Swiss-Prot
Match: Q9DE46 (DNA polymerase alpha catalytic subunit OS=Xenopus laevis OX=8355 GN=pola1 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.8e-63
Identity = 162/492 (32.93%), Postives = 258/492 (52.44%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ-CKEG 60
            MI++  +++ +   +  +V  E+N+ YK LEID+DG++K +LLLKKKKYAA+ ++   +G
Sbjct: 1003 MINTNCNNLEEVFKLGNRVKSEINKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDG 1062

Query: 61   MPYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRG 120
                  E KGLD+VRRDWC L+K+ G++ + QILS    D ++E+I   L +I +++  G
Sbjct: 1063 KYVTKQELKGLDIVRRDWCELAKQAGNYVISQILSDQPRDSIVENIQKKLTEIGENVTNG 1122

Query: 121  QVALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGS 180
             V + +Y I K LTK P+ YPD ++ PHV VA  +   G       GDTI Y+IC     
Sbjct: 1123 TVPITQYEINKALTKDPQDYPDKKSLPHVHVALWINSQGG-RKVKAGDTISYVIC----- 1182

Query: 181  TSGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCL 240
              G +   +QRA   E+L+++     IDT YYLSQQ+HPVV+R+C  I G     +A  L
Sbjct: 1183 QDGSNLSASQRAYAQEQLQKQ-ENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWL 1242

Query: 241  GLDPSKFKIKSCEVFSSDVSSSLV----SCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIF 300
            GLDPS+F  ++   +  D  +  +    S +TDE++Y DC+     CP+C GT       
Sbjct: 1243 GLDPSQF--RAHRHYQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKC-GT------- 1302

Query: 301  SSIYKSTYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVK 360
             +IY + +      +  EP  K  S  +C +   L +V                + N++ 
Sbjct: 1303 ENIYDNVFDGSGLQI--EPGLKRCSKPEC-DASPLDYVI--------------QVHNKLL 1362

Query: 361  MQTDRFIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKA 420
            +   R+I KYY G ++C+E+TC+  T  + L  + +      C K       L   Y + 
Sbjct: 1363 LDIRRYIKKYYSGWLVCEEKTCQNRTRRLPLSFSRNGPICQACSK-----ATLRSEYPEK 1422

Query: 421  DLWKQICYFCHVLDTERCMEKL--EVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYG 480
             L+ Q+C++  + D +  +EK+  E  R    +K   +     K   STV++V  RS Y 
Sbjct: 1423 ALYTQLCFYRFIFDWDYALEKVVSEQERGHLKKKLFQESENQYKKLKSTVDQVLSRSGYS 1455

Query: 481  SIKLKDIAVSLH 486
             + L  +  +L+
Sbjct: 1483 EVNLSKLFQTLN 1455

[Close]

BLAST of MC07g_new0325 vs. NCBI nr
Match: XP_022156119.1 (LOW QUALITY PROTEIN: DNA polymerase alpha catalytic subunit-like [Momordica charantia])

HSP 1 Score: 1012 bits (2617), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM
Sbjct: 743  MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 802

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ
Sbjct: 803  PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 862

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST
Sbjct: 863  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 922

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG
Sbjct: 923  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 982

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK
Sbjct: 983  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 1042

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR
Sbjct: 1043 STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 1102

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ
Sbjct: 1103 FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 1162

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI
Sbjct: 1163 ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 1222

Query: 481  AVSLHMAPC 489
            AVSLHMAPC
Sbjct: 1223 AVSLHMAPC 1231

[Close]

BLAST of MC07g_new0325 vs. NCBI nr
Match: XP_022149463.1 (DNA polymerase alpha catalytic subunit-like [Momordica charantia])

HSP 1 Score: 838 bits (2165), Expect = 4.54e-289
Identity = 409/484 (84.50%), Postives = 441/484 (91.12%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MI+SGLDDI KAKAIA KVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ K+GM
Sbjct: 1068 MIYSGLDDISKAKAIAAKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGM 1127

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMVRRDW LLSKELGDFCL QILSGGSCDDV+ESIHDSLRKIQDD+R+GQ
Sbjct: 1128 PYEVIERKGLDMVRRDWSLLSKELGDFCLSQILSGGSCDDVVESIHDSLRKIQDDMRKGQ 1187

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYII KTLTKPPEAYPDARNQPHVQVAQRLKQ+GY TGC VGDTIPY+ICCEQGST
Sbjct: 1188 VALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYVICCEQGST 1247

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGGSTGIAQRARHP+ELKRE GKWMID DYYLSQQIHPVVSRLCASIQGTSPE LA CLG
Sbjct: 1248 SGGSTGIAQRARHPDELKREDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG 1307

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LD SKF+IKS EV SSDVSSSLV  V+ E+RY  CKPLVLTCP+CY  FEVPTIFSSIYK
Sbjct: 1308 LDSSKFQIKSSEVSSSDVSSSLVFSVSAEERYQGCKPLVLTCPKCYCIFEVPTIFSSIYK 1367

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            STYGKQE P+VDEPT+ FWSN KCP C+DLLWVPDEANA+ RG MT GMISNQVK+QTD+
Sbjct: 1368 STYGKQESPIVDEPTRNFWSNLKCPKCEDLLWVPDEANAS-RGGMTPGMISNQVKIQTDK 1427

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FIAKYYHGLMMCDEETCKY+T  VNL+R GDS++ I CPKYPQCDGRL+RTYT+ADLWKQ
Sbjct: 1428 FIAKYYHGLMMCDEETCKYSTRTVNLRRVGDSQRGIPCPKYPQCDGRLIRTYTEADLWKQ 1487

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            ICYFC VLDTERCMEKLE+H RV LEKEMAKIR LV+LA STV  +R RSAYG +KL+DI
Sbjct: 1488 ICYFCDVLDTERCMEKLEIHTRVTLEKEMAKIRQLVELAVSTVKTIRDRSAYGHLKLEDI 1547

Query: 481  AVSL 484
            AV++
Sbjct: 1548 AVTV 1550

[Close]

BLAST of MC07g_new0325 vs. NCBI nr
Match: XP_022149479.1 (LOW QUALITY PROTEIN: DNA polymerase alpha catalytic subunit-like [Momordica charantia])

HSP 1 Score: 810 bits (2092), Expect = 1.34e-279
Identity = 402/488 (82.38%), Postives = 433/488 (88.73%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDDIG AKAIA KVI EVN+KYKCLEID DGLYKRMLLLKKKKYAAVKLQ K+GM
Sbjct: 934  MIHSGLDDIGNAKAIAAKVIHEVNKKYKCLEIDHDGLYKRMLLLKKKKYAAVKLQLKDGM 993

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMV RDW LLSKELGDFCL QILSGGSCDDVIESIHDSLRKIQDD+R+GQ
Sbjct: 994  PYEVIERKGLDMVHRDWSLLSKELGDFCLSQILSGGSCDDVIESIHDSLRKIQDDMRKGQ 1053

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYII KTLTKPPEAYPDARNQPHVQVAQRLKQ+GY TGC VGDTIPYII CEQGST
Sbjct: 1054 VALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIIFCEQGST 1113

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGG TGIAQRARHP+ELKRE GKWMID DYYLSQQIHPVVSRLCASIQGTSPE LA CLG
Sbjct: 1114 SGGFTGIAQRARHPDELKREDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG 1173

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LD SKF IKS EV +SDVSSSL+  V+ E+RY  CKPLVLTCP+CYG FEVPTIFSSIYK
Sbjct: 1174 LDSSKFLIKSSEVSNSDVSSSLLFSVSAEERYQSCKPLVLTCPKCYGIFEVPTIFSSIYK 1233

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            STYGKQE P+VDEPT KF    KCP C+DLLWVPDEAN  GRG MT  MIS+QVKMQTD+
Sbjct: 1234 STYGKQESPMVDEPTXKF---LKCPKCEDLLWVPDEANE-GRGGMTPEMISSQVKMQTDK 1293

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FIAKYYHGLMMCD+ETCKYTT  VNL+R  DS++ ILCPKYPQCDGRL+RTYT+ADLWKQ
Sbjct: 1294 FIAKYYHGLMMCDKETCKYTTRAVNLRRVSDSQRGILCPKYPQCDGRLIRTYTEADLWKQ 1353

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            ICYF +VLDTE CMEKLE+  RV LEKEMAKIR LV+ AAST+ ++R  +AYG +KL+DI
Sbjct: 1354 ICYFYYVLDTECCMEKLEIRTRVTLEKEMAKIRPLVESAASTIKRIRDLNAYGRVKLEDI 1413

Query: 481  AVSLHMAP 488
            AVSLH+AP
Sbjct: 1414 AVSLHIAP 1417

[Close]

BLAST of MC07g_new0325 vs. NCBI nr
Match: XP_011649321.1 (DNA polymerase alpha catalytic subunit [Cucumis sativus] >KGN61976.1 hypothetical protein Csa_005967 [Cucumis sativus])

HSP 1 Score: 743 bits (1917), Expect = 5.56e-252
Identity = 366/483 (75.78%), Postives = 410/483 (84.89%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDD+GK KAIAGKVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ K+GM
Sbjct: 1074 MIHSGLDDVGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGM 1133

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMVRRDW LLSKELGDFCL QILSGGSC+DV+ESIHDSL KIQ+D+R+GQ
Sbjct: 1134 PYEVIERKGLDMVRRDWSLLSKELGDFCLNQILSGGSCEDVVESIHDSLMKIQEDMRKGQ 1193

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYII KTLTKPPEAYPDARNQPHVQVAQRLKQ+GY TGC VGDTIPYIICCEQ ST
Sbjct: 1194 VALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYTTGCSVGDTIPYIICCEQEST 1253

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGGSTGIAQRARHP+ELK+E GKWMID +YYLSQQIHPVVSRLCASIQGTSPE LA CLG
Sbjct: 1254 SGGSTGIAQRARHPDELKKEDGKWMIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLG 1313

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LD SKF+ +S EV  SD+S+SL+  V DE+RY  C PL  TCP C GTF  P IFSSIYK
Sbjct: 1314 LDSSKFQNRSIEVSRSDISTSLLCSVNDEERYQGCTPLTFTCPSCSGTFNCPPIFSSIYK 1373

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            S  GKQER +VDEPT KFW+N +CP C      PDEANA   GR+T GMI+NQVK Q DR
Sbjct: 1374 SAEGKQER-LVDEPTTKFWNNLRCPKC------PDEANA---GRITPGMIANQVKRQADR 1433

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FI+ YY+GLMMCD+ETCKY T  VNL+  GDS+K  +CP YP C+G LVR YT+ADL+KQ
Sbjct: 1434 FISMYYNGLMMCDDETCKYATRAVNLRVMGDSEKGTICPNYPHCNGHLVRKYTEADLYKQ 1493

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            + YF H+LDTERCMEKLEVH RV LEKEMA IR +V+LAA+T+  +R RSAYG ++L++ 
Sbjct: 1494 LSYFSHILDTERCMEKLEVHARVTLEKEMASIRPVVELAATTIQSIRDRSAYGWVQLQNF 1546

Query: 481  AVS 483
             V+
Sbjct: 1554 VVT 1546

[Close]

BLAST of MC07g_new0325 vs. NCBI nr
Match: KAG6605204.1 (DNA polymerase alpha catalytic subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 736 bits (1900), Expect = 1.97e-249
Identity = 367/482 (76.14%), Postives = 406/482 (84.23%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDDIG+ KAIAGKVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ K+G 
Sbjct: 1075 MIHSGLDDIGQVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGT 1134

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMVRRDW LLSKELGDFCL QILSGGSC+DV ESIHDSL KIQ+D+R+GQ
Sbjct: 1135 PYEVIERKGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVTESIHDSLVKIQEDMRKGQ 1194

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYII KTLTKPPEAYPDARNQPHVQVA RLKQ+GY TGC VGDTIPYIICCEQGST
Sbjct: 1195 VALEKYIITKTLTKPPEAYPDARNQPHVQVALRLKQMGYSTGCSVGDTIPYIICCEQGST 1254

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGGS GIAQRARHP+ELK+E GKWMID DYYLSQQIHPVVSRLCASIQGTSPE LA CLG
Sbjct: 1255 SGGSVGIAQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG 1314

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LD SKF+ KS EV  SDVSSSL+  + DE+RY  C PL LTCP C GTFE P IFSSIYK
Sbjct: 1315 LDSSKFQNKSSEVSRSDVSSSLLCSINDEERYQGCIPLTLTCPSCSGTFECPAIFSSIYK 1374

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            S  GKQE+  VDEPT KFW+N +CP C      PDEA+A   GRMT GMISNQVK Q +R
Sbjct: 1375 SADGKQEK-AVDEPTSKFWNNLRCPKC------PDEASA---GRMTPGMISNQVKRQAER 1434

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FI+ YY+GL+MC++ETCKYTT  VNL+  GDS+K  +CP Y  C+GRL+R YT+ DL+KQ
Sbjct: 1435 FISMYYNGLLMCEDETCKYTTRAVNLRVMGDSEKGTICPNYTHCNGRLIRKYTEVDLYKQ 1494

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            + YF H LDT RCMEKLEVH RV LEKEMAKIR +V+LAAST+  +R RSAYG ++L+D 
Sbjct: 1495 LAYFSHTLDTIRCMEKLEVHARVTLEKEMAKIRPIVELAASTIQSLRDRSAYGWVQLQDF 1546

Query: 481  AV 482
             V
Sbjct: 1555 VV 1546

[Close]

BLAST of MC07g_new0325 vs. ExPASy TrEMBL
Match: A0A6J1DSE0 (DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111023083 PE=3 SV=1)

HSP 1 Score: 1012 bits (2617), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM
Sbjct: 743  MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 802

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ
Sbjct: 803  PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 862

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST
Sbjct: 863  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 922

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG
Sbjct: 923  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 982

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK
Sbjct: 983  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 1042

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR
Sbjct: 1043 STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 1102

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ
Sbjct: 1103 FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 1162

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI
Sbjct: 1163 ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 1222

Query: 481  AVSLHMAPC 489
            AVSLHMAPC
Sbjct: 1223 AVSLHMAPC 1231

[Close]

BLAST of MC07g_new0325 vs. ExPASy TrEMBL
Match: A0A6J1D6V1 (DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017886 PE=3 SV=1)

HSP 1 Score: 838 bits (2165), Expect = 2.20e-289
Identity = 409/484 (84.50%), Postives = 441/484 (91.12%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MI+SGLDDI KAKAIA KVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ K+GM
Sbjct: 1068 MIYSGLDDISKAKAIAAKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGM 1127

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMVRRDW LLSKELGDFCL QILSGGSCDDV+ESIHDSLRKIQDD+R+GQ
Sbjct: 1128 PYEVIERKGLDMVRRDWSLLSKELGDFCLSQILSGGSCDDVVESIHDSLRKIQDDMRKGQ 1187

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYII KTLTKPPEAYPDARNQPHVQVAQRLKQ+GY TGC VGDTIPY+ICCEQGST
Sbjct: 1188 VALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYVICCEQGST 1247

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGGSTGIAQRARHP+ELKRE GKWMID DYYLSQQIHPVVSRLCASIQGTSPE LA CLG
Sbjct: 1248 SGGSTGIAQRARHPDELKREDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG 1307

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LD SKF+IKS EV SSDVSSSLV  V+ E+RY  CKPLVLTCP+CY  FEVPTIFSSIYK
Sbjct: 1308 LDSSKFQIKSSEVSSSDVSSSLVFSVSAEERYQGCKPLVLTCPKCYCIFEVPTIFSSIYK 1367

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            STYGKQE P+VDEPT+ FWSN KCP C+DLLWVPDEANA+ RG MT GMISNQVK+QTD+
Sbjct: 1368 STYGKQESPIVDEPTRNFWSNLKCPKCEDLLWVPDEANAS-RGGMTPGMISNQVKIQTDK 1427

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FIAKYYHGLMMCDEETCKY+T  VNL+R GDS++ I CPKYPQCDGRL+RTYT+ADLWKQ
Sbjct: 1428 FIAKYYHGLMMCDEETCKYSTRTVNLRRVGDSQRGIPCPKYPQCDGRLIRTYTEADLWKQ 1487

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            ICYFC VLDTERCMEKLE+H RV LEKEMAKIR LV+LA STV  +R RSAYG +KL+DI
Sbjct: 1488 ICYFCDVLDTERCMEKLEIHTRVTLEKEMAKIRQLVELAVSTVKTIRDRSAYGHLKLEDI 1547

Query: 481  AVSL 484
            AV++
Sbjct: 1548 AVTV 1550

[Close]

BLAST of MC07g_new0325 vs. ExPASy TrEMBL
Match: A0A6J1D760 (DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017898 PE=3 SV=1)

HSP 1 Score: 810 bits (2092), Expect = 6.50e-280
Identity = 402/488 (82.38%), Postives = 433/488 (88.73%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDDIG AKAIA KVI EVN+KYKCLEID DGLYKRMLLLKKKKYAAVKLQ K+GM
Sbjct: 934  MIHSGLDDIGNAKAIAAKVIHEVNKKYKCLEIDHDGLYKRMLLLKKKKYAAVKLQLKDGM 993

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMV RDW LLSKELGDFCL QILSGGSCDDVIESIHDSLRKIQDD+R+GQ
Sbjct: 994  PYEVIERKGLDMVHRDWSLLSKELGDFCLSQILSGGSCDDVIESIHDSLRKIQDDMRKGQ 1053

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYII KTLTKPPEAYPDARNQPHVQVAQRLKQ+GY TGC VGDTIPYII CEQGST
Sbjct: 1054 VALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYSTGCSVGDTIPYIIFCEQGST 1113

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGG TGIAQRARHP+ELKRE GKWMID DYYLSQQIHPVVSRLCASIQGTSPE LA CLG
Sbjct: 1114 SGGFTGIAQRARHPDELKREDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG 1173

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LD SKF IKS EV +SDVSSSL+  V+ E+RY  CKPLVLTCP+CYG FEVPTIFSSIYK
Sbjct: 1174 LDSSKFLIKSSEVSNSDVSSSLLFSVSAEERYQSCKPLVLTCPKCYGIFEVPTIFSSIYK 1233

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            STYGKQE P+VDEPT KF    KCP C+DLLWVPDEAN  GRG MT  MIS+QVKMQTD+
Sbjct: 1234 STYGKQESPMVDEPTXKF---LKCPKCEDLLWVPDEANE-GRGGMTPEMISSQVKMQTDK 1293

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FIAKYYHGLMMCD+ETCKYTT  VNL+R  DS++ ILCPKYPQCDGRL+RTYT+ADLWKQ
Sbjct: 1294 FIAKYYHGLMMCDKETCKYTTRAVNLRRVSDSQRGILCPKYPQCDGRLIRTYTEADLWKQ 1353

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            ICYF +VLDTE CMEKLE+  RV LEKEMAKIR LV+ AAST+ ++R  +AYG +KL+DI
Sbjct: 1354 ICYFYYVLDTECCMEKLEIRTRVTLEKEMAKIRPLVESAASTIKRIRDLNAYGRVKLEDI 1413

Query: 481  AVSLHMAP 488
            AVSLH+AP
Sbjct: 1414 AVSLHIAP 1417

[Close]

BLAST of MC07g_new0325 vs. ExPASy TrEMBL
Match: A0A0A0LPU1 (DNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_2G278160 PE=3 SV=1)

HSP 1 Score: 743 bits (1917), Expect = 2.69e-252
Identity = 366/483 (75.78%), Postives = 410/483 (84.89%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDD+GK KAIAGKVIQEVN+KYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ K+GM
Sbjct: 1074 MIHSGLDDVGKVKAIAGKVIQEVNKKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGM 1133

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMVRRDW LLSKELGDFCL QILSGGSC+DV+ESIHDSL KIQ+D+R+GQ
Sbjct: 1134 PYEVIERKGLDMVRRDWSLLSKELGDFCLNQILSGGSCEDVVESIHDSLMKIQEDMRKGQ 1193

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            VALEKYII KTLTKPPEAYPDARNQPHVQVAQRLKQ+GY TGC VGDTIPYIICCEQ ST
Sbjct: 1194 VALEKYIITKTLTKPPEAYPDARNQPHVQVAQRLKQMGYTTGCSVGDTIPYIICCEQEST 1253

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGGSTGIAQRARHP+ELK+E GKWMID +YYLSQQIHPVVSRLCASIQGTSPE LA CLG
Sbjct: 1254 SGGSTGIAQRARHPDELKKEDGKWMIDIEYYLSQQIHPVVSRLCASIQGTSPERLADCLG 1313

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LD SKF+ +S EV  SD+S+SL+  V DE+RY  C PL  TCP C GTF  P IFSSIYK
Sbjct: 1314 LDSSKFQNRSIEVSRSDISTSLLCSVNDEERYQGCTPLTFTCPSCSGTFNCPPIFSSIYK 1373

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            S  GKQER +VDEPT KFW+N +CP C      PDEANA   GR+T GMI+NQVK Q DR
Sbjct: 1374 SAEGKQER-LVDEPTTKFWNNLRCPKC------PDEANA---GRITPGMIANQVKRQADR 1433

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FI+ YY+GLMMCD+ETCKY T  VNL+  GDS+K  +CP YP C+G LVR YT+ADL+KQ
Sbjct: 1434 FISMYYNGLMMCDDETCKYATRAVNLRVMGDSEKGTICPNYPHCNGHLVRKYTEADLYKQ 1493

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            + YF H+LDTERCMEKLEVH RV LEKEMA IR +V+LAA+T+  +R RSAYG ++L++ 
Sbjct: 1494 LSYFSHILDTERCMEKLEVHARVTLEKEMASIRPVVELAATTIQSIRDRSAYGWVQLQNF 1546

Query: 481  AVS 483
             V+
Sbjct: 1554 VVT 1546

[Close]

BLAST of MC07g_new0325 vs. ExPASy TrEMBL
Match: A0A6J1G8C4 (DNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111451682 PE=3 SV=1)

HSP 1 Score: 734 bits (1895), Expect = 5.31e-249
Identity = 365/484 (75.41%), Postives = 407/484 (84.09%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDDIG+ KAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQ K+G 
Sbjct: 1075 MIHSGLDDIGQVKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGT 1134

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            PYEVIERKGLDMVRRDW LLSKELGDFCL QILSGGSC+DV ESIHDSL KIQ+D+R+GQ
Sbjct: 1135 PYEVIERKGLDMVRRDWSLLSKELGDFCLSQILSGGSCEDVTESIHDSLVKIQEDMRKGQ 1194

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGST 180
            V LEKYII KTLTKPPEAYPDA+NQPHVQVA RLKQ+GY TGC VGDTIPYIICCEQGST
Sbjct: 1195 VVLEKYIITKTLTKPPEAYPDAKNQPHVQVALRLKQMGYSTGCSVGDTIPYIICCEQGST 1254

Query: 181  SGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCLG 240
            SGGS GIAQRARHP+ELK+E GKWMID DYYLSQQIHPVVSRLCASIQGTSPE LA CLG
Sbjct: 1255 SGGSVGIAQRARHPDELKKEDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSPERLADCLG 1314

Query: 241  LDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIYK 300
            LD SKF+ KS EV  SDVSSSL+  + DE+RY  C PL LTCP C GTFE P IFSSIYK
Sbjct: 1315 LDSSKFQNKSSEVSRSDVSSSLLCSINDEERYQGCIPLTLTCPSCSGTFECPAIFSSIYK 1374

Query: 301  STYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTDR 360
            S  GKQE+  VDEPT KFW+N +CP C      PDEA+A   GRMT GMI+NQVK Q +R
Sbjct: 1375 SADGKQEK-AVDEPTSKFWNNLRCPKC------PDEASA---GRMTPGMIANQVKRQAER 1434

Query: 361  FIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWKQ 420
            FI+ YY+GL+MC++ETCKYTT  VNL+  GDS+K  +CP Y  C+GRL+R YT+ DL+KQ
Sbjct: 1435 FISMYYNGLLMCEDETCKYTTRAVNLRVMGDSEKGTICPNYTHCNGRLIRKYTEVDLYKQ 1494

Query: 421  ICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKDI 480
            + YF H LDT RCMEKLEVH RV LEKEMAKIR +V+LAAST+  +R RSAYG ++L+D 
Sbjct: 1495 LAYFSHTLDTIRCMEKLEVHARVTLEKEMAKIRPIVELAASTIQSLRDRSAYGWVQLQDF 1548

Query: 481  AVSL 484
             V++
Sbjct: 1555 VVTV 1548

[Close]

BLAST of MC07g_new0325 vs. TAIR 10
Match: AT5G67100.1 (DNA-directed DNA polymerases )

HSP 1 Score: 572.0 bits (1473), Expect = 4.6e-163
Identity = 275/483 (56.94%), Postives = 353/483 (73.08%), Query Frame = 0


Query: 1    MIHSGLDDIGKAKAIAGKVIQEVNRKYKCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEGM 60
            MIHSGLDDI + KAI  KVIQEVN+KY+CL+ID DG+YKRMLLL+KKKYAAVKLQ K+G 
Sbjct: 1051 MIHSGLDDIEEVKAIKSKVIQEVNKKYRCLKIDCDGIYKRMLLLRKKKYAAVKLQFKDGK 1110

Query: 61   PYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRGQ 120
            P E IERKG+DMVRRDW LLSKE+GD CL +IL GGSC+DV+E+IH+ L KI++++R GQ
Sbjct: 1111 PCEDIERKGVDMVRRDWSLLSKEIGDLCLSKILYGGSCEDVVEAIHNELMKIKEEMRNGQ 1170

Query: 121  VALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQG-S 180
            VALEKY+I KTLTKPP AYPD+++QPHVQVA R++Q GY  G    DT+PYIIC EQG +
Sbjct: 1171 VALEKYVITKTLTKPPAAYPDSKSQPHVQVALRMRQRGYKEGFNAKDTVPYIICYEQGNA 1230

Query: 181  TSGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAHCL 240
            +S  S GIA+RARHP+E+K EG +W++D DYYL+QQIHPVVSRLCA IQGTSPE LA CL
Sbjct: 1231 SSASSAGIAERARHPDEVKSEGSRWLVDIDYYLAQQIHPVVSRLCAEIQGTSPERLAECL 1290

Query: 241  GLDPSKFKIKSCEVFSSDVSSSLVSCVTDEQRYLDCKPLVLTCPRCYGTFEVPTIFSSIY 300
            GLDPSK++ KS +  SSD S+SL+   +DE+RY  C+PL LTCP C   F  P+I SS+ 
Sbjct: 1291 GLDPSKYRSKSNDATSSDPSTSLLFATSDEERYKSCEPLALTCPSCSTAFNCPSIISSVC 1350

Query: 301  KSTYGKQERPVVDEPTKKFWSNFKCPNCKDLLWVPDEANATGRGRMTCGMISNQVKMQTD 360
             S   K   P  +E    FW    CP C+              G ++  MI+NQVK Q D
Sbjct: 1351 ASISKKPATPETEESDSTFWLKLHCPKCQ---------QEDSTGIISPAMIANQVKRQID 1410

Query: 361  RFIAKYYHGLMMCDEETCKYTTCIVNLQRAGDSKKEILCPKYPQCDGRLVRTYTKADLWK 420
             F++ YY G+M+C++E+CK+TT   N +  G+ ++  +CP YP C+G L+R YT+ADL+K
Sbjct: 1411 GFVSMYYKGIMVCEDESCKHTTRSPNFRLLGERERGTVCPNYPNCNGTLLRKYTEADLYK 1470

Query: 421  QICYFCHVLDTERCMEKLEVHRRVALEKEMAKIRALVKLAASTVNKVRYRSAYGSIKLKD 480
            Q+ YFCH+LDT+  +EK++V  R+ +EK M KIR  VK AA+     R R AYG ++L D
Sbjct: 1471 QLSYFCHILDTQCSLEKMDVGVRIQVEKAMTKIRPAVKSAAAITRSSRDRCAYGWMQLTD 1524

Query: 481  IAV 483
            I +
Sbjct: 1531 IVI 1524

[Close]

BLAST of MC07g_new0325 vs. TAIR 10
Match: AT5G63960.1 (DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases;DNA-directed DNA polymerases )

HSP 1 Score: 78.2 bits (191), Expect = 2.1e-14
Identity = 59/238 (24.79%), Postives = 110/238 (46.22%), Query Frame = 0


Query: 1   MIHSGLDDIGKAKAIAGKVIQEVNRKY-KCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEG 60
           M+  G+ D+  A  +  +  + ++  + K ++++ + +Y   LL+ KK+YA   L     
Sbjct: 758 MVQFGVSDVEAAMTLGREAAEHISGTFIKPIKLEFEKVYFPYLLINKKRYAG--LLWTNP 817

Query: 61  MPYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRG 120
             ++ ++ KG++ VRRD CLL K L    L +IL     D  +    ++++K   D+   
Sbjct: 818 QQFDKMDTKGIETVRRDNCLLVKNLVTESLNKIL----IDRDVPGAAENVKKTISDLLMN 877

Query: 121 QVALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGS 180
           ++ L   +I K LTK  + Y       H ++A+R+++    T   VGD +PY+I      
Sbjct: 878 RIDLSLLVITKGLTKTGDDY--EVKSAHGELAERMRKRDAATAPNVGDRVPYVII----- 937

Query: 181 TSGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAH 238
               + G     R  + +        ID +YYL  QI   + R+   +   + + L H
Sbjct: 938 --KAAKGAKAYERSEDPIYVLQNNIPIDPNYYLENQISKPLLRIFEPVLKNASKELLH 980

[Close]

BLAST of MC07g_new0325 vs. TAIR 10
Match: AT5G63960.2 (DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases;DNA-directed DNA polymerases )

HSP 1 Score: 78.2 bits (191), Expect = 2.1e-14
Identity = 59/238 (24.79%), Postives = 110/238 (46.22%), Query Frame = 0


Query: 1   MIHSGLDDIGKAKAIAGKVIQEVNRKY-KCLEIDLDGLYKRMLLLKKKKYAAVKLQCKEG 60
           M+  G+ D+  A  +  +  + ++  + K ++++ + +Y   LL+ KK+YA   L     
Sbjct: 775 MVQFGVSDVEAAMTLGREAAEHISGTFIKPIKLEFEKVYFPYLLINKKRYAG--LLWTNP 834

Query: 61  MPYEVIERKGLDMVRRDWCLLSKELGDFCLGQILSGGSCDDVIESIHDSLRKIQDDIRRG 120
             ++ ++ KG++ VRRD CLL K L    L +IL     D  +    ++++K   D+   
Sbjct: 835 QQFDKMDTKGIETVRRDNCLLVKNLVTESLNKIL----IDRDVPGAAENVKKTISDLLMN 894

Query: 121 QVALEKYIIRKTLTKPPEAYPDARNQPHVQVAQRLKQIGYLTGCYVGDTIPYIICCEQGS 180
           ++ L   +I K LTK  + Y       H ++A+R+++    T   VGD +PY+I      
Sbjct: 895 RIDLSLLVITKGLTKTGDDY--EVKSAHGELAERMRKRDAATAPNVGDRVPYVII----- 954

Query: 181 TSGGSTGIAQRARHPEELKREGGKWMIDTDYYLSQQIHPVVSRLCASIQGTSPECLAH 238
               + G     R  + +        ID +YYL  QI   + R+   +   + + L H
Sbjct: 955 --KAAKGAKAYERSEDPIYVLQNNIPIDPNYYLENQISKPLLRIFEPVLKNASKELLH 997

The following BLAST results are available for this feature:

BLAST of MC07g_new0325 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48653	3.4e-163	59.88	DNA polymerase alpha catalytic subunit OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FHA3	6.4e-162	56.94	DNA polymerase alpha catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=POLA P...	[more]
P09884	2.3e-66	34.29	DNA polymerase alpha catalytic subunit OS=Homo sapiens OX=9606 GN=POLA1 PE=1 SV=...	[more]
O89042	2.1e-64	33.91	DNA polymerase alpha catalytic subunit (Fragment) OS=Rattus norvegicus OX=10116 ...	[more]
Q9DE46	1.8e-63	32.93	DNA polymerase alpha catalytic subunit OS=Xenopus laevis OX=8355 GN=pola1 PE=1 S...	[more]

BLAST of MC07g_new0325 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156119.1	0.0	100.00	LOW QUALITY PROTEIN: DNA polymerase alpha catalytic subunit-like [Momordica char...	[more]
XP_022149463.1	4.54e-289	84.50	DNA polymerase alpha catalytic subunit-like [Momordica charantia]	[more]
XP_022149479.1	1.34e-279	82.38	LOW QUALITY PROTEIN: DNA polymerase alpha catalytic subunit-like [Momordica char...	[more]
XP_011649321.1	5.56e-252	75.78	DNA polymerase alpha catalytic subunit [Cucumis sativus] >KGN61976.1 hypothetica...	[more]
KAG6605204.1	1.97e-249	76.14	DNA polymerase alpha catalytic subunit, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of MC07g_new0325 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DSE0	0.0	100.00	DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111023083 PE=3 SV=1	[more]
A0A6J1D6V1	2.20e-289	84.50	DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017886 PE=3 SV=1	[more]
A0A6J1D760	6.50e-280	82.38	DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017898 PE=3 SV=1	[more]
A0A0A0LPU1	2.69e-252	75.78	DNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_2G278160 PE=3 SV=1	[more]
A0A6J1G8C4	5.31e-249	75.41	DNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111451682 PE=3 SV=1	[more]

BLAST of MC07g_new0325 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67100.1	4.6e-163	56.94	DNA-directed DNA polymerases	[more]
AT5G63960.1	2.1e-14	24.79	DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases...	[more]
AT5G63960.2	2.1e-14	24.79	DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 20..40
None	No IPR available	PANTHER	PTHR45861	DNA POLYMERASE ALPHA CATALYTIC SUBUNIT	coord: 1..482
None	No IPR available	PANTHER	PTHR45861:SF2	DNA POLYMERASE	coord: 1..482
IPR015088	Zinc finger, DNA-directed DNA polymerase, family B, alpha	PFAM	PF08996	zf-DNA_Pol	coord: 264..480 e-value: 6.2E-36 score: 123.9
IPR042087	DNA polymerase family B, thumb domain	GENE3D	1.10.132.60		coord: 72..253 e-value: 8.2E-60 score: 203.5
IPR038256	DNA polymerase alpha, zinc finger domain superfamily	GENE3D	1.10.3200.20	DNA Polymerase alpha, zinc finger	coord: 260..473 e-value: 1.3E-40 score: 140.8
IPR006134	DNA-directed DNA polymerase, family B, multifunctional domain	PFAM	PF00136	DNA_pol_B	coord: 1..226 e-value: 2.1E-47 score: 161.9
IPR023211	DNA polymerase, palm domain superfamily	GENE3D	3.90.1600.10	Palm domain of DNA polymerase	coord: 1..71 e-value: 9.2E-13 score: 50.5
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 1..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g_new0325.1	MC07g_new0325.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0006273	lagging strand elongation
biological_process	GO:0006272	leading strand elongation
biological_process	GO:0009965	leaf morphogenesis
biological_process	GO:1902975	mitotic DNA replication initiation
biological_process	GO:0006260	DNA replication
biological_process	GO:0006269	DNA replication, synthesis of RNA primer
cellular_component	GO:0005658	alpha DNA polymerase:primase complex
molecular_function	GO:0017076	purine nucleotide binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0019103	pyrimidine nucleotide binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003688	DNA replication origin binding
molecular_function	GO:0003896	DNA primase activity
molecular_function	GO:0003682	chromatin binding
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0000166	nucleotide binding